CN103027004A - Long-distance transportation method of sepia esculenta adults - Google Patents
Long-distance transportation method of sepia esculenta adults Download PDFInfo
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- CN103027004A CN103027004A CN2013100079324A CN201310007932A CN103027004A CN 103027004 A CN103027004 A CN 103027004A CN 2013100079324 A CN2013100079324 A CN 2013100079324A CN 201310007932 A CN201310007932 A CN 201310007932A CN 103027004 A CN103027004 A CN 103027004A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a long-distance transportation method of sepia esculenta adults, and belongs to the field of aquatic product transportation. The long-distance transportation method comprises the steps of two-step oxygenation and two-step anesthetization, wherein the transportation time is not more than 20h; the two-step oxygenation comprises the following steps: oxygenating water for transporting sepia esculenta in a water reservoir with a nano-airstone until saturation, and oxygenating a transportation bag after the sepia esculenta adults are placed into the transportation bag; and the two-step anesthetization comprises the following steps: adding 30-60mg/L of MS-222 anesthetic to a sepia esculenta culture pond without disturbing the sepia esculenta adults, and instantly weighing and packing for transportation when the sepia esculenta adults are anesthetized until the sepia esculenta adults stop swinging the lateral fins and lose the sense of balance; and adding 5-8mg/L of MS-222 anesthetic to the water for transportation, so that the sepia esculenta adults maintain a two-stage anesthetic transportation state, namely, the sepia esculenta adults slightly lose the touch sense, fold the wrists, swing the fins naturally, and changes the body color slightly. With adoption of the long-distance transportation method of the sepia esculenta adults, the survival rate of the sepia esculenta adults during long-distance transportation is significantly increased, and the technical problem about the long-distance transportation of the sepia esculenta adults is solved.
Description
Technical field
The invention belongs to the aquatic product transportation field, relate to particularly a kind of golden cuttlefish adult long-distance transport method.
Background technology
Under field conditions (factors), when facing a danger, ink-jet is cephalopodous a kind of escape behavior, near the seawater of the prepared Chinese ink of ejection can making becomes black, thereby hidden own, and by the current escape of quick ejection, prepared Chinese ink also contains toxin in addition, and harmful animal is also had certain attacking role.In breeding process, find equally, during fight between parent purchase, fall pond operation and individuality siphonopods also as easy as rolling off a log generation ink-jet behavior, especially long-distance transport front pack, weigh and transportation in the ink-jet phenomenon even more serious.The ink-jet behavior of siphonopods in transportation not only damages physique, also ruins easily water quality, causes transportation survival rate lower, limited to a certain extent the development of its industry.
Aspect siphonopods research, abroad focus mostly in basic research such as Resources of Cephalopods investigation and biological propertyes, China is being in rank first aspect the siphonopods aquaculture.At present, the principal item of domestic siphonopods exploitation cultivation is golden cuttlefish, Sepiella maindroni, octopus (true octopus, short octopus and long octopus) etc.In these kinds, the octopus class adopts the low-temperature transport survival rate very high because without inner casing, health is soft, and ink ejection amount is little, does not have the transportation technology bottleneck.And golden cuttlefish and Sepiella maindroni have hard inner casing, and be not low temperature resistant, and irritant reaction is responsive to external world, and as easy as rolling off a log ink-jet and collision in transportation are finally ruined because of water quality or somatic damage death.Both compare, and the golden cuttlefish individuality is larger, is 4～5 times of Sepiella maindroni, therefore ink ejection amount is larger, and golden cuttlefish has tailpin, also easier damage and cause tailpin and inner casing exposes of health tail muscles after the collision, so golden cuttlefish long-distance transport difficulty is larger.At present golden cuttlefish is mainly take short-distance transport as main, and method is mainly used for reference fish plastic sack oxygenation transportation, but the method golden cuttlefish 4～5 hours ink-jet rate up to more than 60%, transportation survival rate is extremely low, only is 40%.Therefore golden cuttlefish long-distance transport technology has limited the popularization cultivation of golden cuttlefish to a certain extent, is the bottleneck of its Industry Promotion.
Anaesthetic can make aquatic livestock quiet, and then the stress reaction when reducing operations such as adopting ovum, semen collection, blood sampling, so that operate smooth.In addition, in transportation, anaesthetic can reduce aquatic livestock metabolism, reduce the injury to animal bodies, and then improve the transportation survival rate.Therefore, anaesthetic obtains certain application in aquaculture.Fish is more with anaesthetic at present, comprise nearly 30 kinds of MS-222, sulfuric acid quinaldine, eugenol, 2-Phenoxyethanol etc., MS-222(chemistry alkylsulfonate coordination benzocaine by name wherein) has aquatic livestock and enter the advantages such as fast and recovery time of fiber crops is short, that the our times various countries are widely used in safe, reliable, the most effective anaesthetic in the aquatic livestock, and approval process FDA(food and drug administration) is used for aquatic livestock anesthesia transportation.
Aspect the siphonopods transportation, Japanology squid live body long-distance transport method, mainly contain three kinds: 1) remove ink sac; 2) use ethene and the seawater that adds special oxide, and water temperature is controlled at about 5 ℃; 3) with-2 ℃ seawater " hypothermic anesthesia ".Although adopt these methods to transport for long-distance, shorter through the survival of the squid after the long-distance transport, only be 12h～1 week, so these methods are unwell to the transportation of siphonopods parent.At home, the principle of the employings such as Wu Changwen anesthesia has been invented a kind of siphonopods parent live transport pre-treating method and special agent (application number: 201010607873.0), opened up a new road for siphonopods transports.But because the siphonopods kind is more, there is interspecific difference in different cultivars, anesthesia kind selection, concentration, narcosis etc. are also not the same, and the zeolite granular of selecting in this invention in addition only absorbs ammonia nitrogen and prepared Chinese ink, but can't remove the toxin that is dissolved into after the ink-jet in the water.
That golden cuttlefish has is individual large, ink ejection amount is large, oxygen demand is large, stress reaction is fierce and inner casing tool tailpin very easily causes the characteristics such as somatic damage, therefore control not ink-jet in golden cuttlefish operation and the transportation, improve the transportation water-soluble oxygen, keeping the transportation peace and quiet is the keys that improve its long-distance transport survival rate.Have no at present the report of golden cuttlefish adult long-distance transport method both at home and abroad.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of golden cuttlefish adult long-distance transport method, selects best narcosis that golden cuttlefish is operated and transports, to reduce the generation of ink-jet behavior; Improve the transportation water-soluble oxygen; Keep transportation quiet, with the reduction stress reaction, and then solve low this technical bottleneck of golden cuttlefish long-distance transport survival rate.
The present invention finishes by following operating technology:
A kind of golden cuttlefish adult long-distance transport method comprises the step that two step oxygenations and two steps anaesthetize, and haulage time is no more than 20h;
Described two step oxygenations: to before the water extraction that is used for the transportation golden cuttlefish at tank with the oxygenation of nanometer gas stone to saturated, after golden cuttlefish is put into shipping bags again in the shipping bags oxygenation;
Described two steps anesthesia: in the situation that does not alarm golden cuttlefish, the MS-222 anaesthetic that adds 30～60mg/L in the golden cuttlefish culturing pool is anaesthetized to 4 phases of narcosis when golden cuttlefish, and namely wrist scatters, lateral fin stops swinging, and the sense of equilibrium weighs immediately in when forfeiture and packs transportation; The MS-222 anaesthetic that in the transportation water, adds 5～8mg/L, so that golden cuttlefish is kept 2 phases of narcosis, i.e. sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
Further, the MS-222 anaesthetic that in the golden cuttlefish culturing pool, adds 30～40mg/L.
Further, described shipping bags is double-deck black polyethylene plastic bag.
Further, the water temperature of the transportation water in the described shipping bags is 16～18 ℃, and the oxygen that is filled with in the shipping bags is 2:1～4:1 with the volume ratio of transportation water.
Further, before the golden cuttlefish culturing pool is added anaesthetic, at first the temperature of breeding water is dropped to 16 ~ 18 ℃.
Further, described cool-down method is for adding the cooling of ice cube or ice chest, and through 2～3 step by step cooling processing, each temperature difference must not be above 3～5 ℃.
The beneficial effect that the present invention is compared with the prior art:
1) clear and definite first golden cuttlefish anesthesia level has been determined suitable anesthetic concentration and mode of operation by stages, and the narcosis of narcotic concentration and golden cuttlefish during transportation.
2) the present invention adopts the step of two steps anesthesia, namely to carrying out the high concentration anesthesia procedure before the golden cuttlefish transportation, low concentration anesthesia in the transportation, not only controlled the generation of ink-jet behavior when the pond, weigh and packing, avoid the physique damage, also reduced the transportation consumption rate, controlled the ink-jet behavior in the transportation, ensured transportation water quality, and then significantly improved golden cuttlefish long-distance transport survival rate, solved golden cuttlefish adult long-distance transport technical barrier.
3) the Promethean two step oxygenation steps that adopted of the present invention, namely in bulk container, adopt nanometer gas stone will transport water and fill pure oxygen to saturated, then join and carry out again the oxygenation sealing in the plastic sack, 7mg/L when dissolved oxygen in water is directly sealed the plastic sack oxygenation by prior art is increased to 15mg/L, has significantly improved the dissolved oxygen amount of transportation water.
4) the present invention transports the double-deck white polyethylene plastic sack that generally adopts with the oxygenation of aquatic livestock plastic sack and changes double-deck black polyethylene plastic bag into, played shaded effect in the transportation, thereby make golden cuttlefish calm, with minimizing collision behavior, and then reduced shipping damage.
5) adopt the present invention to transport the golden cuttlefish adult, it is longer to cultivate the time-to-live after the transportation, until natural death does not affect parent mating breeding.
Description of drawings
The MS-222 anaesthetic of Fig. 1 variable concentrations is to the final narcosis of golden cuttlefish
Embodiment
Be described in detail technical method of the present invention below by embodiment:
(1) according to golden cuttlefish anesthesia and recovery behavior by stages, then determines suitable golden cuttlefish anesthesia procedure concentration and anesthesia transportation concentration
Result of the test: 1) according to the behavioural characteristic under the final narcosis anesthesia level is divided into six periods, sees Table 1.
Table 1 anesthesia level by stages and behavioural characteristic
| Anesthesia level by stages | |
| 0 phase | Wrist closes up, and fin swings normal, the nature that moves about, and body colour is normal |
| 1 phase | Sense of touch is slightly lost, and wrist closes up, and fin swings normal, the nature that moves about, and body colour is normal |
| 2 phases | Sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes |
| 3 phases | Lateral fin swings to be accelerated, and it is rapid to move about, and wrist closes up, and body colour reddens |
| 4 phases | Wrist scatters, and lateral fin stops swinging, sense of equilibrium's |
| 5 phases | The ink-jet behavior occurs |
| 6 phases | Sign of life stops, individual death |
2) according to the recovery feature recovery process is divided into four periods, sees Table 2.
Table 2 recovery process by stages and behavioural characteristic
| The recovery process by stages | |
| 1 phase | Swim in the water surface, lateral fin is motionless, and wrist opens |
| 2 phases | Swim in the water surface, lateral fin begins to swing |
| 3 phases | Swim in the water surface, wrist closes up gradually |
| 4 phases | Beginning in lower water layer move about |
According to golden cuttlefish anesthesia and recovery behavior state feature, determine that through experimental verification repeatedly the suitable operation narcosis of MS-222 anesthesia golden cuttlefish was 4 phases, namely wrist scatters, and lateral fin stops swinging, sense of equilibrium's forfeiture; As shown in Figure 1, anaesthetic concentration is 30～60mg/L in the breeding water body, and golden cuttlefish all can reach 4 phases of narcosis, and the used time is 3～5min; Suitable anesthesia transportation concentration 5～8mg/L, narcosis was 2 phases, i.e. and sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
Embodiment 2:
In June, 2012, we are transported to prosperous Yongfeng, Tianjin aquaculture Co., Ltd to 250 golden cuttlefish adults by Golden Beach, Qingdao aquatic products development corporation, Ltd..The transportation water is cooled to 18 ℃ with ice cube by 24 ℃, fills pure oxygen to saturated with nanometer gas stone behind the MS-222 of adding 7mg/L; Add ice cube or ice chest cooling in the golden cuttlefish culturing pool that will stop eating 1 day, through 2 step by step cooling processing, each temperature difference must not surpass 3 ℃, cools the temperature to 18 ℃; Then add the MS-222 that final concentration is 30mg/L, golden cuttlefish enters 4 phases of narcosis behind the 5min, and namely wrist scatters, lateral fin stops swinging, sense of equilibrium's forfeiture is dragged for golden cuttlefish to washbowl immediately gently, slowly pulls out with hand after weighing and puts into the black plastic bag that 10L transportation water is housed, put 1 of golden cuttlefish adult for every bag, squeeze out the air in the bag, be filled with about 30L oxygen, rubber band is put into bubble chamber after tightening sack, every case is put 2 bags, then adds 2 freezing mineral water bottles.Namely put into thermal car after with adhesive tape bubble chamber being obturaged and transport, compartment temperature keeps 17～19 ℃ in the transportation.Arrive behind the Tianjin and plastic sack to be put into 20 ℃ seawater transition preparing after 15 minutes, then open bag golden cuttlefish is poured in the fresh seawater.11 hours to Hangu, Tianjin transportation result shows from the Qingdao land transportation, and golden cuttlefish transportation ink-jet rate only is 2%, and survival rate is up to 96%, thereby efficiently solves golden cuttlefish long-distance transport technical barrier.
Embodiment 3:
In July, 2012, we are transported to Xin Hai prosperous nursery in Xiapu, Fujian to 150 golden cuttlefish adults by Huanghai Sea aquatic products research institute Huang Island golden cuttlefish Experimental Base.The transportation water adds the MS-222 of 8mg/L with ice block cooling to 16 ℃, then fills pure oxygen to saturated with nanometer gas stone; Add ice cube or ice chest cooling in the golden cuttlefish culturing pool that will stop eating 1 day, through 3 step by step cooling processing, each temperature difference is no more than 5 ℃, cools the temperature to 16 ℃; Then add the MS-222 that final concentration is 60mg/L, golden cuttlefish enters 4 phases of narcosis behind the 3min, and namely wrist scatters, lateral fin stops swinging, sense of equilibrium's forfeiture is dragged for golden cuttlefish to washbowl immediately gently, slowly pulls out with hand after weighing and puts into the black plastic bag that 10L transportation water is housed, put 1 of golden cuttlefish adult for every bag, squeeze out the air in the bag, be filled with about 30L oxygen, rubber band is put into bubble chamber after tightening sack, every case is put 2 bags, then adds 2 freezing mineral water bottles.Namely put into thermal car after with adhesive tape bubble chamber being obturaged and transport, compartment temperature keeps about 16 ℃ in the transportation.Arrive behind the Fujian and plastic sack to be put into 20 ℃ seawater transition preparing after 15 minutes, then open bag golden cuttlefish is poured in the fresh seawater.15 hours to Xiapu, Fujian transportation result shows from the Qingdao land transportation, and transportation ink-jet rate only is 3%, and survival rate is up to 94%, and this golden cuttlefish long-distance transport technology is further verified.
Claims (6)
1. a golden cuttlefish adult long-distance transport method is characterized in that it comprises the step of two step oxygenations and the anesthesia of two steps, and haulage time is no more than 20h;
Described two step oxygenations: to before the water extraction that is used for the transportation golden cuttlefish at tank with the oxygenation of nanometer gas stone to saturated, secondly after golden cuttlefish is put into shipping bags again in the shipping bags oxygenation;
Described two steps anesthesia: in the situation that does not alarm golden cuttlefish, the MS-222 anaesthetic that adds 30～60mg/L in the golden cuttlefish culturing pool is anaesthetized to 4 phases of narcosis when golden cuttlefish, and namely wrist scatters, lateral fin stops swinging, and the sense of equilibrium weighs immediately in when forfeiture and packs; The MS-222 anaesthetic that in the transportation water, adds 5～8mg/L, so that golden cuttlefish is kept anesthesia 2 phases of travel position, i.e. sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
2. a kind of golden cuttlefish adult long-distance transport method according to claim 1 is characterized in that adding the MS-222 anaesthetic of 30～40mg/L in the golden cuttlefish culturing pool.
3. a kind of golden cuttlefish adult long-distance transport method according to claim 1 is characterized in that described shipping bags is double-deck black polyethylene plastic bag.
4. a kind of golden cuttlefish adult long-distance transport method according to claim 1 is characterized in that the water temperature of the transportation water in the described shipping bags is 16～18 ℃, and the oxygen that is filled with in the shipping bags is 2:1～4:1 with the volume ratio of transportation water.
5. a kind of golden cuttlefish adult long-distance transport method according to claim 1 is characterized in that at first the temperature of breeding water being dropped to 16～18 ℃ before the golden cuttlefish culturing pool is added anaesthetic.
6. a kind of golden cuttlefish adult long-distance transport method according to claim 5 is characterized in that described cool-down method is for adding the cooling of ice cube or ice chest, through 2～3 step by step cooling processing, 3～5 ℃ of each temperature difference.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103798171A (en) * | 2014-02-22 | 2014-05-21 | 上海海洋大学 | No-water transportation method of aquatic product |
| CN103843711A (en) * | 2014-04-03 | 2014-06-11 | 山东东方海洋科技股份有限公司 | Conveying method for trout |
| CN104145860A (en) * | 2014-08-01 | 2014-11-19 | 中国水产科学研究院长江水产研究所 | Rhinogobio ventralis parent fish transporting method |
| CN104521810A (en) * | 2014-12-04 | 2015-04-22 | 中国科学院南海海洋研究所 | Anesthesia and transport method for sea horses |
| CN104996339A (en) * | 2015-07-24 | 2015-10-28 | 浙江师范大学 | Pre-treatment mixed reagent for transportation of symphysodon aequfasciatuslive bodies |
| CN105052810A (en) * | 2015-09-09 | 2015-11-18 | 岭南师范学院 | Kisslip cuttlefish fry long-distance alive-keeping transport method |
| CN105210943A (en) * | 2015-10-08 | 2016-01-06 | 山东省海洋生物研究院 | A kind of spot trout live fish hypothermic anesthesia transportation resources |
| CN105325322A (en) * | 2014-08-12 | 2016-02-17 | 中国科学院水生生物研究所 | Method improving Mongolia culter fry transportation survival rate |
| CN111387091A (en) * | 2020-05-25 | 2020-07-10 | 中国水产科学研究院黄海水产研究所 | Long-distance transportation method of adult Sepiella maindroni |
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| CN103798171A (en) * | 2014-02-22 | 2014-05-21 | 上海海洋大学 | No-water transportation method of aquatic product |
| CN103843711A (en) * | 2014-04-03 | 2014-06-11 | 山东东方海洋科技股份有限公司 | Conveying method for trout |
| CN103843711B (en) * | 2014-04-03 | 2015-12-30 | 山东东方海洋科技股份有限公司 | The transportation resources of a kind of salmon trout |
| CN104145860A (en) * | 2014-08-01 | 2014-11-19 | 中国水产科学研究院长江水产研究所 | Rhinogobio ventralis parent fish transporting method |
| CN104145860B (en) * | 2014-08-01 | 2016-04-06 | 中国水产科学研究院长江水产研究所 | A kind of long Qi Wen Minnow parent population transportation resources |
| CN105325322A (en) * | 2014-08-12 | 2016-02-17 | 中国科学院水生生物研究所 | Method improving Mongolia culter fry transportation survival rate |
| CN104521810A (en) * | 2014-12-04 | 2015-04-22 | 中国科学院南海海洋研究所 | Anesthesia and transport method for sea horses |
| CN104996339A (en) * | 2015-07-24 | 2015-10-28 | 浙江师范大学 | Pre-treatment mixed reagent for transportation of symphysodon aequfasciatuslive bodies |
| CN105052810A (en) * | 2015-09-09 | 2015-11-18 | 岭南师范学院 | Kisslip cuttlefish fry long-distance alive-keeping transport method |
| CN105210943A (en) * | 2015-10-08 | 2016-01-06 | 山东省海洋生物研究院 | A kind of spot trout live fish hypothermic anesthesia transportation resources |
| CN105210943B (en) * | 2015-10-08 | 2018-07-24 | 山东省海洋生物研究院 | A kind of spot trout live fish hypothermic anesthesia transportation resources |
| CN111387091A (en) * | 2020-05-25 | 2020-07-10 | 中国水产科学研究院黄海水产研究所 | Long-distance transportation method of adult Sepiella maindroni |
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