CN111387091A - Long-distance transportation method of adult Sepiella maindroni - Google Patents
Long-distance transportation method of adult Sepiella maindroni Download PDFInfo
- Publication number
- CN111387091A CN111387091A CN202010447395.5A CN202010447395A CN111387091A CN 111387091 A CN111387091 A CN 111387091A CN 202010447395 A CN202010447395 A CN 202010447395A CN 111387091 A CN111387091 A CN 111387091A
- Authority
- CN
- China
- Prior art keywords
- adult
- transport
- cuttlefish
- seawater
- putting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241001663378 Sepiella maindroni Species 0.000 title claims description 8
- 239000013535 sea water Substances 0.000 claims description 21
- 241000238371 Sepiidae Species 0.000 claims description 19
- 241000238366 Cephalopoda Species 0.000 claims description 12
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 4
- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 claims description 3
- 229960003760 florfenicol Drugs 0.000 claims description 3
- 210000001835 viscera Anatomy 0.000 claims description 3
- 241001663395 Sepiella Species 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 230000009182 swimming Effects 0.000 claims description 2
- 206010002091 Anaesthesia Diseases 0.000 abstract description 8
- 230000037005 anaesthesia Effects 0.000 abstract description 8
- 230000036284 oxygen consumption Effects 0.000 abstract description 2
- 241000222722 Leishmania <genus> Species 0.000 abstract 1
- 241000238370 Sepia Species 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 6
- 238000011084 recovery Methods 0.000 description 5
- 230000017448 oviposition Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/02—Receptacles specially adapted for transporting live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a long-distance transportation method of a sepia leishmania adult, belonging to the field of aquatic product transportation. The method of the invention adopts high-concentration anesthesia operation and low-concentration anesthesia transportation, thoroughly solves the ink-jet phenomenon in the transportation process, effectively avoids operation damage and reduces the oxygen consumption rate in transportation.
Description
Technical Field
The invention belongs to the field of aquatic product transportation, and particularly relates to a long-distance transportation method of a cuttlefish adult Leishae.
Background
Under natural conditions, when a danger is met, ink jet is an escape behavior of cephalopods, the sprayed ink can enable nearby seawater to become black, so that the ink conceals the self and escapes by means of fast sprayed water flow, and in addition, the ink contains toxin and has a certain attack effect on enemies. In the culture process, the cephalopods are also easy to jet ink when the parents purchase, the pond pouring operation and the fighting between individuals, and particularly the ink jet phenomenon is more serious in the processes of bagging, weighing and transporting before long-distance transportation. The ink-jet behavior of the cephalopods in the transportation process not only damages physique, but also easily destroys water quality, once ink is jetted in the transportation process, the death rate is almost 100 percent, and the ink-jet phenomenon in the transportation process limits the development of the cephalopod industry to a certain extent.
In the aspect of cephalopod transportation, most of the prior methods refer to fish transportation, namely, the plastic bags are subjected to oxygen charging transportation after cooling, but the ink jet rate in 4-5 hours is up to more than 80%, only individual plastic bag parts without ink jet survive, and the transportation survival rate is extremely low. Wu Heiguang and the like invent a cephalopodium parent living body transportation pretreatment method and a special reagent (application number: 201010607873.0) by adopting the principle of anesthesia, open up a new path for cephalopodium transportation, but different varieties have species difference, if some varieties have developed ink sacs, large ink jet amount, different anesthesia type selection, concentration and anesthesia states, and the like are not consistent, and in addition, the selected zeolite particles only absorb ammonia nitrogen and ink but cannot eliminate toxins dissolved in water after ink jet. Although the method can be used for long-distance transportation, the operation is complicated, the technical requirement is high, the damage to the cuttlefish is large, the survival time of the cuttlefish after long-distance transportation is short, and the transported parent cannot be bred, so that the method is not suitable for transportation of the cephalopod parent. Therefore, the long-distance transportation method for cephalopods, particularly for sepiales and gun-shaped cephalopods, is simple to operate, small in damage to organisms and high in transportation survival rate.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a long-distance transportation method of a Sepiella maindroni adult.
The invention is realized by adopting the following technical scheme:
a long-distance transportation method of adult Sepiella maindroni comprises the following steps:
1) fishing out the cuttlefish adults stopped eating for 1-2 days from the culture pond with water, then putting the cuttlefish adults into a magnesium chloride solution prepared from natural seawater and having a mass ratio of 0.75-1%, anaesthetizing for 8-10 min, and fishing out and weighing the cuttlefish adults after the cuttlefish adults slow swimming;
2) after weighing, the adult Sepiella maindroni faces upwards, the front end of the outer sleeve cavity is squeezed towards the middle along two sides by hands, viscera are exposed, and the transparent part of the ink bag opening is tied or clamped by a ligation device;
3) putting the adult Leishi squid into transport bags, filling seawater containing magnesium chloride with the mass ratio of 0.04-0.06%, carrying out oxygen charging and transport in the transport bags, putting 1 adult Leishi squid in each transport bag, putting 2 transport bags in each box, and then adding ice blocks for cooling; the temperature of the seawater in the transport bag is 15-17 ℃, and the volume ratio of the oxygen filled in the transport bag to the transport seawater is 2: 1-4: 1;
4) after the cuttlefish arrives at a transportation destination, loosening a ligation device at an ink bag opening, putting the cuttlefish adult Leishae into seawater at the temperature of 18-20 ℃ for resuscitation, and if an ink jet phenomenon is found, replacing fresh seawater;
5) after the sepiella reiliana adult recovers for 2-3 hours, 8-12 ppm of florfenicol is used for medicated bath for 1 hour to prevent infection.
Compared with the prior art, the invention has the beneficial effects that:
1. the method of the invention thoroughly solves the ink jet phenomenon in the transportation process and obviously improves the transportation survival rate.
2. The high-concentration anesthesia operation and the low-concentration anesthesia transportation are adopted in the anesthesia method, so that the operation damage is effectively avoided, and the oxygen consumption rate of transportation is reduced.
Detailed Description
The technical solution of the present invention is further explained by the following examples, but the scope of the present invention is not limited in any way by the examples.
Example 1
1) Taking the adult (average weight is 800g) of the cuttlefish from the culture pond with water for 1-2 days, putting the adult into a magnesium chloride solution prepared from natural seawater and having a mass ratio of 0.75%, anesthetizing for 8-10 min, taking out and weighing after the adult of the cuttlefish slows down;
2) after weighing, the adult Sepiella maindroni is pressed towards the middle along two sides by hand at the front end of the outer sleeve cavity with the belly facing upwards to expose viscera, and the mouth part (transparent part) of the ink sac is tied in a triple knot or multiple knots by using an arc needle (model 1/23 x8) of a medical suture needle with a thread of a ligation device, or the mouth part (transparent part) of the ink sac is clamped by using a clip needle.
3. Putting the adult Leishi into seawater containing 0.05% of magnesium chloride, carrying out oxygen-charging transportation by plastic bags, putting 1 adult Leishi in each bag, putting 2 bags in each box, and then adding a small amount of ice blocks for cooling. The seawater temperature of the transport water is 15-17 ℃, the volume ratio of oxygen filled in the transport bag to the transport seawater is 2: 1-4: 1.
4. the live fish transport vehicle with the constant-temperature refrigeration function is adopted for transporting, the transport time is 10-14 h, the ink-jet phenomenon is not found, and the transport survival rate is 100%. After the cuttlefish is transported to the destination, the thin line is cut off by small scissors, or the clip is taken off, and then the cuttlefish is put into seawater for resuscitation. The temperature difference between the recovery seawater and the transportation seawater is not more than 3 ℃, and if an ink jet phenomenon is found in the recovery process, the fresh seawater is immediately replaced. 4-5 hours after recovery, the wound infection is prevented by using 10ppm florfenicol for medicated bath for 1 hour. The ink jet rate is 60-80% during the recovery period, and the survival rate after 24h of recovery is 100%.
5. Transferring the recovered adults for more than 24 hours into a parent culture pond, putting branches into the parent culture pond as an egg laying attachment base, controlling the illumination intensity at 400 lx-800 lx, the water temperature at 25-26 ℃, the salinity at 28-30 and the density at 3-4/m2The water changing rate is 500-600%, and the bait is live shrimp. 180 egg-laying sheaths are averagely produced in the breeding period, and the egg-laying sheaths do not show the untransferred parentsThe difference is significant.
Claims (1)
1. A long-distance transportation method of adult Sepiella maindroni is characterized by comprising the following steps:
1) fishing out the cuttlefish adults stopped eating for 1-2 days from the culture pond with water, then putting the cuttlefish adults into a magnesium chloride solution prepared from natural seawater and having a mass ratio of 0.75-1%, anaesthetizing for 8-10 min, and fishing out and weighing the cuttlefish adults after the cuttlefish adults slow swimming;
2) after weighing, the adult Sepiella maindroni faces upwards, the front end of the outer sleeve cavity is squeezed towards the middle along two sides by hands, viscera are exposed, and the transparent part of the ink bag opening is tied or clamped by a ligation device;
3) putting the adult Leishi squid into transport bags, filling seawater containing magnesium chloride with the mass ratio of 0.04-0.06%, carrying out oxygen charging and transport in the transport bags, putting 1 adult Leishi squid in each transport bag, putting 2 transport bags in each box, and then adding ice blocks for cooling; the temperature of the seawater in the transport bag is 15-17 ℃, and the volume ratio of the oxygen filled in the transport bag to the transport seawater is 2: 1-4: 1;
4) after the cuttlefish arrives at a transportation destination, loosening a ligation device at an ink bag opening, putting the cuttlefish adult Leishae into seawater at the temperature of 18-20 ℃ for resuscitation, and if an ink jet phenomenon is found, replacing fresh seawater;
5) after the sepiella reiliana adult recovers for 2-3 hours, 8-12 ppm of florfenicol is used for medicated bath for 1 hour to prevent infection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010447395.5A CN111387091A (en) | 2020-05-25 | 2020-05-25 | Long-distance transportation method of adult Sepiella maindroni |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010447395.5A CN111387091A (en) | 2020-05-25 | 2020-05-25 | Long-distance transportation method of adult Sepiella maindroni |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111387091A true CN111387091A (en) | 2020-07-10 |
Family
ID=71411924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010447395.5A Pending CN111387091A (en) | 2020-05-25 | 2020-05-25 | Long-distance transportation method of adult Sepiella maindroni |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111387091A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113749020A (en) * | 2021-10-12 | 2021-12-07 | 中国水产科学研究院黄海水产研究所 | Method for breeding parent of sepia ducreyi |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005211075A (en) * | 2005-03-08 | 2005-08-11 | Ichibansha:Kk | Method for treating live cuttlefish, device for treating live cuttlefish and squid ink-discharged live cuttlefish |
| JP2009072116A (en) * | 2007-09-20 | 2009-04-09 | Kagoshima Prefecture | Tool for preventing leakage and ejection of ink from ink sac of cuttlefish, method for preventing leak and discharge of ink from ink sac of cuttlefish, and cuttlefish prevented from leakage and discharge of ink |
| CN101703026A (en) * | 2009-11-17 | 2010-05-12 | 浙江海洋学院 | Squid living body transportation method and special ink control chute |
| CN201640203U (en) * | 2009-11-17 | 2010-11-24 | 浙江海洋学院 | Squid pre-shipment treatment plant |
| CN102113480A (en) * | 2010-12-15 | 2011-07-06 | 浙江海洋学院 | Pretreatment method and special reagent for living body transportation of parents of cephalopods |
| CN103027004A (en) * | 2013-01-09 | 2013-04-10 | 中国海洋大学 | Long-distance transportation method of sepia esculenta adults |
| CN105052810A (en) * | 2015-09-09 | 2015-11-18 | 岭南师范学院 | Kisslip cuttlefish fry long-distance alive-keeping transport method |
-
2020
- 2020-05-25 CN CN202010447395.5A patent/CN111387091A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005211075A (en) * | 2005-03-08 | 2005-08-11 | Ichibansha:Kk | Method for treating live cuttlefish, device for treating live cuttlefish and squid ink-discharged live cuttlefish |
| JP2009072116A (en) * | 2007-09-20 | 2009-04-09 | Kagoshima Prefecture | Tool for preventing leakage and ejection of ink from ink sac of cuttlefish, method for preventing leak and discharge of ink from ink sac of cuttlefish, and cuttlefish prevented from leakage and discharge of ink |
| CN101703026A (en) * | 2009-11-17 | 2010-05-12 | 浙江海洋学院 | Squid living body transportation method and special ink control chute |
| CN201640203U (en) * | 2009-11-17 | 2010-11-24 | 浙江海洋学院 | Squid pre-shipment treatment plant |
| CN102113480A (en) * | 2010-12-15 | 2011-07-06 | 浙江海洋学院 | Pretreatment method and special reagent for living body transportation of parents of cephalopods |
| CN103027004A (en) * | 2013-01-09 | 2013-04-10 | 中国海洋大学 | Long-distance transportation method of sepia esculenta adults |
| CN105052810A (en) * | 2015-09-09 | 2015-11-18 | 岭南师范学院 | Kisslip cuttlefish fry long-distance alive-keeping transport method |
Non-Patent Citations (2)
| Title |
|---|
| 任淑仙 等: "《动物的类群》", 28 February 1983, 人民教育出版社 * |
| 陈令明 等: "金乌贼亲本长短途运输的注意要点", 《水产养殖》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113749020A (en) * | 2021-10-12 | 2021-12-07 | 中国水产科学研究院黄海水产研究所 | Method for breeding parent of sepia ducreyi |
| CN113749020B (en) * | 2021-10-12 | 2022-05-13 | 中国水产科学研究院黄海水产研究所 | Method for breeding parent of sepia duchensis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103027004B (en) | Long-distance transportation method of sepia esculenta adults | |
| Booke et al. | Sodium bicarbonate, an inexpensive fish anesthetic for field use | |
| CN105210943B (en) | A kind of spot trout live fish hypothermic anesthesia transportation resources | |
| KR102060915B1 (en) | Seawater domestication and inland seawater aquaculture method of atlantic salmon | |
| CN105191833A (en) | Method for keeping large number of freshwater fishes alive without water | |
| CN104381166B (en) | Application of fishery electrochemical water in transporting fish larvae of tilapia mossambica | |
| CN104521810B (en) | A kind of hippocampus anesthesia and transportation resources | |
| CN111387091A (en) | Long-distance transportation method of adult Sepiella maindroni | |
| CN108419723A (en) | A kind of carbon dioxide narcosis method of seawater grouper | |
| Braid et al. | Incidence of cannibalism among striped bass fry in an intensive culture system | |
| KR100814511B1 (en) | The method for making salted fish and shellfish using salt produced with deep sea water | |
| CN102960784A (en) | Machining method of frozen cuttlefish | |
| CN106879538A (en) | A kind of cultural method of the giant salamander | |
| CN205357783U (en) | Live body aquatic livestock conveyer | |
| AU2008213166A1 (en) | Waterless storage and transport of live aquatic animals | |
| Smith et al. | Shipping studies with juvenile and adult Malaysian prawns Macrobrachium rosenbergii (De Man) | |
| CN108207762A (en) | A kind of low power consuming does not increase nitrogen content method while increasing oxygen content of water | |
| CN113287549A (en) | Sea-lowering domestication cultivation method for freshwater aquaculture silver salmon seeds | |
| JP2000175595A (en) | Transportation of live fish | |
| CN108633790B (en) | Anesthesia transportation method for seriolala quinqueradiata seedlings | |
| CN104396815A (en) | Application of fishery electrochemical water in adult grass carp or crucian transportation | |
| Suwetja et al. | Handling Technique Development of Live Carp, Cyprinus carpio, In Cold Dry Styrofoam Box | |
| CN108432666A (en) | Lai Shi intends cuttlefish fertilized eggs long-distance transport method | |
| KR101287148B1 (en) | Method of preserving decapsulated cyst and storage medium for preserving decapsulated cyst | |
| JPH11341934A (en) | Transportation and maintenance of fish and shellfish and prolongation of hatching period of embryonic egg |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200710 |