CN103013647A - Method for extracting soybean oil by using aqueous enzymatic method - Google Patents
Method for extracting soybean oil by using aqueous enzymatic method Download PDFInfo
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- CN103013647A CN103013647A CN2012105780975A CN201210578097A CN103013647A CN 103013647 A CN103013647 A CN 103013647A CN 2012105780975 A CN2012105780975 A CN 2012105780975A CN 201210578097 A CN201210578097 A CN 201210578097A CN 103013647 A CN103013647 A CN 103013647A
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Abstract
The invention relates to a method for extracting soybean oil by using an aqueous enzymatic method, belonging to the vegetable oil extraction technology. The method comprises the following steps of: (1), after crushing soybean, carrying out vacuum extruding and puffing pre-treatment to obtain a puffed material, and freezing the puffed material after crushing; (2), mixing the frozen puffed material with water to obtain a mixed solution; (3), carrying out enzymolysis by adding protex-6L alkaline protease into the mixed solution to obtain an enzymolysis solution; and (4), after deactivating the enzymolysis solution, centrifugally separating to obtain free oil, emulsion, hydrolysate and residues, and separating and standing the free oil to obtain the soybean oil. The method disclosed by the invention has the characteristics of being simple in process, safe for operation, little in emulsion, high in yield of the free oil and the like.
Description
Technical field
The invention belongs to Vegetable oil lipoprotein and extract processing technology, relate generally to a kind of method of aqueous enzymatic extraction soybean oil.
Background technology
Aqueous enzymatic method is put forward oil (EAEP) technology and is carried oil tech as a kind of emerging " green, environmental protection ", and it can return when extracting grease efficiently
Receive other value components in the oil plant, be called " a kind of complete utilization technology of oil resource " by grease scientific circles, aqueous enzymatic method is carried oil tech and is compared with traditional technology, has significant advantage at aspects such as energy consumption, environment and safety and sanitation.
In the aqueous enzymatic extraction soybean oil process, because the emulsification of albumen and grease is so that the free oil yield is lower, therefore, demulsification directly affects the soybean oil yield behind enzymolysis, but breakdown of emulsion efficient is not very high at present.
Summary of the invention
Technical problem to be solved by this invention is to overcome above-mentioned the deficiencies in the prior art, and a kind of method of aqueous enzymatic extraction soybean oil is provided, and reaches the purpose that reduces milk sap, improves the free oil yield.
Technical problem to be solved by this invention is achieved through the following technical solutions:
(1) soybean is pulverized the expanded pre-treatment of laggard row vacuum extrusion and obtains expanded material, will carry out freezing treatment behind the expanded crushing material, and described freezing temp is-30--20 ℃, freezing time is 10-12h; (2) expanded material and water after freezing are mixed to get mixed solution, described amount of water is 6 times of quality of material; (3) adding the protex-6L Sumizyme MP in the mixed solution carries out enzymolysis and obtains enzymolysis solution; (4) enzymolysis solution is gone out centrifugation behind the enzyme obtains free oil, milk sap, hydrolyzed solution and residue, namely gets soybean oil after free oil is separated, leaves standstill.
The expanded nib of described vacuum extrusion aperture is 18-22mm, and screw speed is 80-120 r/min, and extrusion temperature is 80 ℃-90 ℃, and material water ratio is 10%-15%, and vacuum tightness is 0.06-0.08MPa.
Described enzyme digestion reaction enzyme concentration is 1% of quality of material, and hydrolysis temperature is 50-55 ℃, and enzymolysis time is 3-3.5 h, and enzymolysis pH is 9.0-9.5.
The present invention adopts vacuum puffed pre-treatment that the aqueous enzymatic method raw material is carried out pre-treatment to be increased its cytoclasis degree, reduces protein denaturation, and the raw material after expanded is carried out the content that freezing treatment can reduce milk sap behind the enzymolysis greatly, and then the yield of raising free oil, it is simple that present method has technique, operational safety, gained milk sap is few, free oil yield high.
Description of drawings
Accompanying drawing 1 is overall process route figure of the present invention.
Specific embodiments
Below in conjunction with accompanying drawing the specific embodiment of the invention is described in detail.
A kind of method of aqueous enzymatic extraction soybean oil, the method may further comprise the steps: (1) soybean is pulverized the expanded pre-treatment of laggard row vacuum extrusion and obtains expanded material, to carry out freezing treatment behind the expanded crushing material, described freezing temp is-30--20 ℃, freezing time is 10-12h; (2) expanded material and water after freezing are mixed to get mixed solution, described amount of water is 6 times of quality of material; (3) adding the protex-6L Sumizyme MP in the mixed solution carries out enzymolysis and obtains enzymolysis solution; (4) enzymolysis solution is gone out centrifugation behind the enzyme obtains free oil, milk sap, hydrolyzed solution and residue, namely gets soybean oil after free oil is separated, leaves standstill.
The expanded nib of described vacuum extrusion aperture is 18-22mm, and screw speed is 80-120 r/min, and extrusion temperature is 80 ℃-90 ℃, and material water ratio is 10%-15%, and vacuum tightness is 0.06-0.08MPa.
Described enzyme digestion reaction enzyme concentration is 1% of quality of material, and hydrolysis temperature is 50-55 ℃, and enzymolysis time is 3-3.5 h, and enzymolysis pH is 9.0-9.5.
Embodiment 1
Soybean is pulverized the rear moisture content of regulating reach 14%, it is 90 ℃ in the vacuum extrusion temperature, vacuum tightness is 0.07 MPa, screw speed 100 r/min nib apertures are to carry out the expanded pre-treatment of vacuum extrusion under the 18mm condition to obtain expanded material, with expanded material freezing 12h under-25 ℃, take by weighing expanded material and the 1200ml water of 200g after freezing and be mixed to get mixed solution, in mixed solution, add the protex-6L Sumizyme MP of 2ml at 55 ℃, obtain enzymolysis solution behind the enzymolysis 3.5h under the pH9.2 condition, with the enzymolysis solution centrifugation behind the enzyme of going out, obtain free oil, milk sap, hydrolyzed solution and residue, free oil is separated, namely get soybean oil after leaving standstill, the free oil yield is 62%.
Embodiment 2
Soybean is pulverized the rear moisture content of regulating reach 12%, it is 90 ℃ in the vacuum extrusion temperature, vacuum tightness is 0.07 MPa, screw speed 100 r/min nib apertures are to carry out the expanded pre-treatment of vacuum extrusion under the 18mm condition to obtain expanded material, with expanded material freezing 10h under-20 ℃, take by weighing expanded material and the 1200ml water of 200g after freezing and be mixed to get mixed solution, in mixed solution, add the protex-6L Sumizyme MP of 2ml at 50 ℃, obtain enzymolysis solution behind the enzymolysis 3.0h under the pH9.0 condition, with the enzymolysis solution centrifugation behind the enzyme of going out, obtain free oil, milk sap, hydrolyzed solution and residue, free oil is separated, namely get soybean oil after leaving standstill, the free oil yield is 58%.
Embodiment 3
Soybean is pulverized the rear moisture content of regulating reach 12%, it is 90 ℃ in the vacuum extrusion temperature, vacuum tightness is 0.07 MPa, screw speed 100 r/min nib apertures are to carry out the expanded pre-treatment of vacuum extrusion under the 18mm condition to obtain expanded material, with expanded material freezing 12h under-30 ℃, take by weighing expanded material and the 1200ml water of 200g after freezing and be mixed to get mixed solution, in mixed solution, add the protex-6L Sumizyme MP of 2ml at 60 ℃, obtain enzymolysis solution behind the enzymolysis 3.0h under the pH9.5 condition, with the enzymolysis solution centrifugation behind the enzyme of going out, obtain free oil, milk sap, hydrolyzed solution and residue, free oil is separated, namely get soybean oil after leaving standstill, the free oil yield is 63%.
Claims (3)
1. the method for an aqueous enzymatic extraction soybean oil, it is characterized in that, the method may further comprise the steps: (1) soybean is pulverized the expanded pre-treatment of laggard row vacuum extrusion and obtains expanded material, freezing treatment will be carried out behind the expanded crushing material, described freezing temp is-30--20 ℃, and freezing time is 10-12h; (2) expanded material and water after freezing are mixed to get mixed solution, described amount of water is 6 times of quality of material; (3) adding the protex-6L Sumizyme MP in the mixed solution carries out enzymolysis and obtains enzymolysis solution; (4) enzymolysis solution is gone out centrifugation behind the enzyme obtains free oil, milk sap, hydrolyzed solution and residue, namely gets soybean oil after free oil is separated, leaves standstill.
2. the method for a kind of aqueous enzymatic extraction soybean oil according to claim 1, it is characterized in that the expanded nib of described vacuum extrusion aperture is 18-22mm, screw speed is 80-120 r/min, extrusion temperature is 80 ℃-90 ℃, material water ratio is 10%-15%, and vacuum tightness is 0.06-0.08MPa.
3. the method for a kind of aqueous enzymatic extraction soybean oil according to claim 1 is characterized in that described enzyme digestion reaction enzyme concentration is 1% of quality of material, and hydrolysis temperature is 50-55 ℃, and enzymolysis time is 3-3.5 h, and enzymolysis pH is 9.0-9.5.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255585A (en) * | 2015-11-12 | 2016-01-20 | 东北农业大学 | Preparation method of xanthoceras sorbifolia bunge oil and xanthoceras sorbifolia bunge protein peptide |
| CN106039319A (en) * | 2016-06-06 | 2016-10-26 | 东北农业大学 | Method for preparing water-insoluble drug supported fat emulsion from soybean emulsion with aqueous enzymatic method |
| CN107245368A (en) * | 2017-06-13 | 2017-10-13 | 广西仙珠食品有限公司 | A kind of method for extracting soybean grease |
| CN107384574A (en) * | 2017-06-13 | 2017-11-24 | 广西仙珠食品有限公司 | A kind of method for extracting soybean grease |
| CN108641801A (en) * | 2018-04-11 | 2018-10-12 | 安徽德运粮油有限公司 | A method of enzymolysis and extraction soybean oil is handled using vacuum flashing twice |
| CN108913328A (en) * | 2018-08-04 | 2018-11-30 | 望江县振兴植物油厂（普通合伙） | A kind of preparation process of nutrient blend oil |
| CN109232705A (en) * | 2018-08-30 | 2019-01-18 | 佛山市欧若拉生物科技有限公司 | A kind of processing technology of soyasaponin extract |
| CN113122366A (en) * | 2019-12-31 | 2021-07-16 | 丰益(上海)生物技术研发中心有限公司 | Peanut processing method and peanut oil |
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| CN101602979A (en) * | 2009-06-09 | 2009-12-16 | 东北农业大学 | A kind of extracting method of soybean oil |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255585A (en) * | 2015-11-12 | 2016-01-20 | 东北农业大学 | Preparation method of xanthoceras sorbifolia bunge oil and xanthoceras sorbifolia bunge protein peptide |
| CN106039319A (en) * | 2016-06-06 | 2016-10-26 | 东北农业大学 | Method for preparing water-insoluble drug supported fat emulsion from soybean emulsion with aqueous enzymatic method |
| CN107245368A (en) * | 2017-06-13 | 2017-10-13 | 广西仙珠食品有限公司 | A kind of method for extracting soybean grease |
| CN107384574A (en) * | 2017-06-13 | 2017-11-24 | 广西仙珠食品有限公司 | A kind of method for extracting soybean grease |
| CN108641801A (en) * | 2018-04-11 | 2018-10-12 | 安徽德运粮油有限公司 | A method of enzymolysis and extraction soybean oil is handled using vacuum flashing twice |
| CN108913328A (en) * | 2018-08-04 | 2018-11-30 | 望江县振兴植物油厂（普通合伙） | A kind of preparation process of nutrient blend oil |
| CN109232705A (en) * | 2018-08-30 | 2019-01-18 | 佛山市欧若拉生物科技有限公司 | A kind of processing technology of soyasaponin extract |
| CN113122366A (en) * | 2019-12-31 | 2021-07-16 | 丰益(上海)生物技术研发中心有限公司 | Peanut processing method and peanut oil |
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| CN103013647B (en) | 2014-10-29 |
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Effective date of registration: 20180110 Address after: 257000 Kenli Economic Development Zone, east of Huafeng Road, Kenli Economic Development Zone, Dongying City, Shandong Province, north of Honghong River Patentee after: Shandong Wonderful Industrial Group Co., Ltd. Address before: 150030 Xiangfang City, Harbin Province Wood Street, No. 59, No. Patentee before: Northeast Agricultural University |
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Effective date of registration: 20191122 Address after: 257000 east of Huafeng road and north of Yihong River, Kenli District, Dongying City, Shandong Province Co-patentee after: Shandong Wan De Fu Biotechnology Co., Ltd. Patentee after: Shandong Wonderful Industrial Group Co., Ltd. Address before: 257000 Kenli Economic Development Zone, east of Huafeng Road, Kenli Economic Development Zone, Dongying City, Shandong Province, north of Honghong River Patentee before: Shandong Wonderful Industrial Group Co., Ltd. |
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Effective date of registration: 20200217 Address after: 257000 east of Huafeng road and north of Yihong River, Kenli District, Dongying City, Shandong Province Patentee after: Shandong Wan De Fu Biotechnology Co., Ltd. Address before: 257000 east of Huafeng road and north of Yihong River, Kenli District, Dongying City, Shandong Province Co-patentee before: Shandong Wan De Fu Biotechnology Co., Ltd. Patentee before: Shandong Wonderful Industrial Group Co., Ltd. |
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