CN103013435B - 一种利用棉粕制备蛋白胶的方法 - Google Patents
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- CN103013435B CN103013435B CN201210521655.4A CN201210521655A CN103013435B CN 103013435 B CN103013435 B CN 103013435B CN 201210521655 A CN201210521655 A CN 201210521655A CN 103013435 B CN103013435 B CN 103013435B
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 44
- 102000009027 Albumins Human genes 0.000 title claims abstract description 22
- 108010088751 Albumins Proteins 0.000 title claims abstract description 22
- 239000003292 glue Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 102000004882 Lipase Human genes 0.000 claims abstract description 8
- 108090001060 Lipase Proteins 0.000 claims abstract description 8
- 239000004367 Lipase Substances 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 235000019421 lipase Nutrition 0.000 claims abstract description 8
- 235000011121 sodium hydroxide Nutrition 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 229940088598 enzyme Drugs 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 238000005516 engineering process Methods 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241001529246 Platymiscium Species 0.000 description 1
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229930000755 gossypol Natural products 0.000 description 1
- 229950005277 gossypol Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000011120 plywood Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000020978 protein processing Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- 239000002023 wood Substances 0.000 description 1
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Abstract
一种利用棉粕制备蛋白胶的方法，将棉粕粉碎过200目筛，加水，棉粕与水的质量比为1:4，添加纤维素酶、脂肪酶进行酶解，待灭酶活后加入烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶。该方法得到的蛋白胶更加细腻，粘度更高。
Description
技术领域
本发明涉及一种利用棉粕制备蛋白胶的方法，属植物提取领域。
技术背景
棉粕是将棉籽压榨并将残油分离后得到的，棉粕中含有40%以上的粗蛋白质及20%左右的粗脂肪和粗纤维。棉粕通常用于饲料，但是棉粕中粗蛋白质的组成不均衡，而且通常含有游离棉酚对动物的毒性较大，因此饲料并不是棉粕最理想的用途。本发明将棉粕中的蛋白加工成蛋白胶，可用于人造板的粘合剂。
传统工艺中，直接采用蛋白碱变性处理棉粕，致使棉粕中的纤维素、脂肪等物质对蛋白胶黏剂的粘度造成影响，传统工艺通常采用将棉粕粉碎过200目筛以上的方式以减少纤维素、脂肪对蛋白胶粘度的影响，但是机械粉碎能耗大、效率低。本发明对传统工艺进行改进，得到的蛋白胶更加细腻，粘度更高。
发明内容
本发明的目的是提供一种利用棉粕制备蛋白胶的方法，该方法得到的蛋白胶更加细腻，粘度更高。
为实现以上目的，本发明的一种利用棉粕制备蛋白胶的方法，是将棉粕粉碎过200目筛，加水，棉粕与水的质量比为1:4，添加棉粕干重0.06%-0.08%的纤维素酶、棉粕干重0.03%-0.05%的脂肪酶，按常规工艺进行酶解。待灭酶活后加入棉粕干重4%-6%的烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶。
本发明对传统工艺进行改进，首先用纤维素酶及脂肪酶将棉粕中的纤维素与脂肪充分分解，再结合蛋白碱变性处理，得到的蛋白胶更加细腻，粘度更高。
实施例
实施例1：
将棉粕粉碎过200目筛，加水，棉粕与水的质量比为1:4，添加棉粕干重0.06%的纤维素酶、棉粕干重0.03%的脂肪酶进行酶解。待灭酶活后加入棉粕干重6%的烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶。
实施例2：
将棉粕粉碎过200目筛，加水，棉粕与水的质量比为1:4，添加棉粕干重0.07%的纤维素酶、棉粕干重0.04%的脂肪酶进行酶解。待灭酶活后加入棉粕干重5%的烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶。
实施例3：
将棉粕粉碎过200目筛，加水，棉粕与水的质量比为1:4，添加棉粕干重0.08%的纤维素酶、棉粕干重0.05%的脂肪酶进行酶解。待灭酶活后加入棉粕干重4%的烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶。
分别测定上述3个实施例制备的蛋白胶的粘度，同时将上述3种蛋白胶用于制备三合板，测定板材的抗拉强度，测的结果如表1所示。
表1.蛋白胶的粘度及所制板材的抗拉强度
| 抗拉强度(MPa) | 粘度(PaS) | |
| 实施例1 | 2.548 | 78.23 |
| 实施例2 | 2.632 | 78.65 |
| 实施例3 | 2.671 | 78.99 |
Claims (1)
1.一种利用棉粕制备蛋白胶的方法，其特征在于：将棉粕粉碎过200目筛， 加水，棉粕与水的质量比为1︰4，添加纤维素酶、脂肪酶进行酶解，待灭酶活后加入烧碱，50℃下恒温水浴加热30min，搅拌混合均匀制成蛋白胶；所述的纤维素 酶添加量为棉粕干重的0.06%-0.08%，所述的脂肪酶添加量为棉粕干重的0.03%-0. 05%；所述的烧碱的添加量为棉粕干重的4%-6%。
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| CN103709972B (zh) * | 2013-12-10 | 2015-07-22 | 福建农林大学 | 一种室温固化的耐候性植物蛋白胶黏剂及其制备方法 |
| CN112920771B (zh) * | 2021-01-28 | 2022-03-25 | 山东农业大学 | 一种自交联棉籽蛋白胶黏剂及其制备方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295902A (zh) * | 2011-06-24 | 2011-12-28 | 浙江农林大学 | 油茶粕蛋白胶粘剂的生产方法 |
| CN102304334A (zh) * | 2011-08-08 | 2012-01-04 | 北京林业大学 | 一种改性麻疯树粕胶粘剂及其制备方法 |
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| CN102295902A (zh) * | 2011-06-24 | 2011-12-28 | 浙江农林大学 | 油茶粕蛋白胶粘剂的生产方法 |
| CN102304334A (zh) * | 2011-08-08 | 2012-01-04 | 北京林业大学 | 一种改性麻疯树粕胶粘剂及其制备方法 |
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Inventor after: Liu Kaihua Inventor before: Su Jianli |
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Effective date of registration: 20170818 Address after: 710404 Jixian Industrial Park, Zhouzhi County, Shaanxi, Xi'an Patentee after: Zhouzhi County Tianyi biological science and Technology Co.,Ltd. Address before: 266061 Shandong, Laoshan, Hongkong East Road, No. Pioneering Park, biological park, room 248, No. 239 Patentee before: QINGDAO WINCHANCE TECHNOLOGY Co.,Ltd. |
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Denomination of invention: A Method of Preparing Protein Glue from Cotton Meal Effective date of registration: 20221110 Granted publication date: 20151021 Pledgee: Xi'an Caijin Financing Guarantee Co.,Ltd. Pledgor: Zhouzhi County Tianyi biological science and Technology Co.,Ltd. Registration number: Y2022610000743 |
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