CN103006347A - Fixing device for suckling mouse brain extraction - Google Patents
Fixing device for suckling mouse brain extraction Download PDFInfo
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- CN103006347A CN103006347A CN2012105485955A CN201210548595A CN103006347A CN 103006347 A CN103006347 A CN 103006347A CN 2012105485955 A CN2012105485955 A CN 2012105485955A CN 201210548595 A CN201210548595 A CN 201210548595A CN 103006347 A CN103006347 A CN 103006347A
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- fixing device
- flat dish
- neonatal rat
- extraction
- brain
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Abstract
The invention discloses a fixing device for suckling mouse brain extraction. The fixing device comprises a black base flat dish, wherein four brackets which are uniformly distributed are arranged at the center of the base flat dish, an operating flat dish is supported by the four brackets together, a rough soft plastic thick film covers the bottom of the operating flat dish, two upright columns made from soft plastics are arranged on a plane of the bottom of the operating flat dish, a clamping groove exists in the middle of the two upright columns, and holes communicated with the base flat dish are formed in the clamping groove. The fixing device for the suckling mouse brain extraction has the important significance that the quality for suckling mouse brain tissue extraction can be effectively improved, the time for extraction is shortened, the pollution is reduced, the depletion rate of fine forceps is reduced, the workload for researchers is reduced, the fixing device is reusable, the cost for experimental consumables is reduced, and the like. Shown by document retrieval carried out by the applicant, similar devices of the same type do not exist yet.
Description
Technical field
The invention belongs to the fundamental biological knowledge experimental provision, relate to a kind of equipment of preclinical medicine animal brain cell culture midbrain tissue extraction, be specifically related to a kind of neonatal rat and get the brain fixture.
Background technology
Animal cell culture, the particularly brain cell of the cerebral tissue such as cortex, Hippocampus (neuron, glial cell and vascular endothelial cell) are cultivated and are widely used in nervous system and the developmental biology research.Utilize to cultivate successful cell and make various nervous system various diseases (brain injury, epilepsy and degeneration etc.) model, become the pathophysiological mechanism of studying above-mentioned disease and guiding clinical treatment important tool it
In the neonatal rat body bark fetching layer, Hippocampus etc. to be organized into last cell culture success, molding be a complexity, time-consuming flow process, it is meticulous, rapid that each step requires, the step of particularly getting cerebral tissue is more crucial.What of later stage cell quantity are this step not only determine, and affect greatly the activity of brain cell and survival rate etc.At present, the concrete steps of getting brain are: neonatal rat is positioned in a dry glass ware or the plastics plate, in microscopically or perusal, extracts rapidly required cerebral tissue according to the region of anatomy with meticulous tweezers.
At present, have no on the market the cost performance height, easy to use, more effective assurance is extracted the neonatal rat of brain tissue cell quality and quantity and is got the brain fixture, and in the extraction cerebral tissue process, there are following shortcoming fixing neonatal rat in the glass dish of using at present or plastics plate:
1) glass dish or plastics plate are generally transparent, aberration contrast extreme difference, and when generally getting brain, the plate bottom lining black scraps of paper or select the microscope of black base use extremely inconveniently, repeat to change the scraps of paper, and are uneconomical;
2) glass dish or plastics plate bottom light are sliding, and neonatal rat is fixing not good, particularly can't be upright, prolong the operating time greatly, and it is active to affect cerebral tissue, and cost performance is low;
3) glass dish or plastics plate bottom light are sliding, and neonatal rat is fixing not good, and the meticulous tweezers of normal operation are fixed, and make it keep a certain position.At first neonatal rat is relatively more fragile, and meticulous tweezers destroy more tissue performance fixation, poor effect easily; Secondly, meticulous tweezers are very expensive, and its tip is very meticulous, and the plate bottom is hard, is very easy to damage in fixation procedure the tip of tweezers, and the proportion of goods damageds are high, cause great waste;
4) extract brain cell purity for anti-Hemostatic Oral Liquid impact, generally select dry glass ware or plastics plate, but in the neonatal rat situation how, its blood with it can be cumulative, the suckling mouse brain that causes the later stage to be extracted is organized influenced larger, activity decreased, poor effect.
The literature search of doing through the applicant, up to now till, yet there are no the report of similar similar installation.
Summary of the invention
Defective or deficiency for above-mentioned prior art exists the object of the invention is to, and provide a kind of neonatal rat to get the brain fixture, to finish better, more rapidly the quality and quantity that extracts cerebral tissue and improve brain tissue cell.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of neonatal rat is got the brain fixture, it is characterized in that, the base plate that comprises a black, central authorities at the base plate are provided with 4 equally distributed supports, 4 supports support an operation plate jointly, and operation plate bottom is coated with coarse flexible plastic thick film, operates the column that has 2 flexible plastics to make on the plane of plate bottom, in the middle of two columns a draw-in groove is arranged, the hole that is connected with the base plate is arranged in the draw-in groove.
Among the present invention, the base plate is made by rustless steel; Support is made by rustless steel; The operation plate is made by the good tempered glass of light transmission; Thick film and two columns that operation plate bottom covers are made by flexible plastic.
Neonatal rat of the present invention is got the brain fixture, when extracting cerebral tissue, can effectively neonatal rat be fixed in the middle of the groove of two columns, in the time of rapid high efficiency extraction cerebral tissue, be discharged into rapidly in the base plate with oxygen containing DMEM or PBS flushing liquor, reduce unnecessary blood contamination, have the quality of extracting cerebral tissue, shorten extraction time, reduce and pollute, reduce the coefficient of losses of meticulous tweezers, alleviate research worker workload and Reusability, and it is all significant to reduce experiment consumptive material expense etc.Through the literature search that the applicant does, yet there are no similar similar installation.
Description of drawings
Fig. 1 is that neonatal rat of the present invention is got brain fixture structure sketch map;
Fig. 2 is the metamorphosis picture of the neuron different time points of cultivation, wherein
The present invention is described in further detail below in conjunction with drawings and Examples.
The specific embodiment
Referring to accompanying drawing 1, present embodiment provides a kind of neonatal rat and gets the brain fixture, comprise a base plate 1, central authorities at base plate 1 are provided with 4 supports 2, these 4 support 2 upper supports have an operation plate 3, and operation plate 3 bottoms are coated with coarse flexible plastic thick film 4, are fixed with two columns 5 on the plane of operation plate 3 bottoms, in the middle of two columns 5 draw-in groove 6 is arranged, the hole 7 that is connected with base plate 1 is arranged in the draw-in groove 6.
Black is made and made to base plate 1 by rustless steel; Support 2 is made by rustless steel; Operation plate 3 is made by the good tempered glass of light transmission; The material of thick film 4 and column 5 is selected flexible plastic.
During use, under cryogenic conditions, the straight torso of neonatal rat on thick film 4, is fixed on the head of neonatal rat in the draw-in groove 6 of two columns 5, available oxygen containing DMEM or PBS liquid wash neonatal rat head, waste liquid is discharged in the base plate 1 along hole 7.Simultaneously, base plate 1 not only for the operation plate 3 black background is provided, and the ice sheet that freezes in it also can be the operation plate 3 low temperature environment is provided, the protection cerebral tissue; Thick film 4 can be protected the meticulous tweezers that use in the operating process.
Adopt neonatal rat of the present invention to get the brain fixture, fix, when peeling off suckling mouse brain, cultured cells is referring to Fig. 2; Table 1 is to use neonatal rat of the present invention to get the brain fixture, is instructing neonatal rat to fix, get brain, is peeling off the relative analysis data that reach and do not use this invention with the cultured cell situation.Results suggest can shorten greatly gets brain, splitting time, and the cultured cells quality is better.
Table 1: use the contrast of the present invention and untapped indices
Subscript a represents P＜0.05.
Claims (5)
1. a neonatal rat is got the brain fixture, the base plate (1) that comprises a black, central authorities at base plate (1) are provided with 4 equally distributed supports (2), 4 supports (2) support an operation plate (3) jointly, the bottom of operation plate (3) is coated with coarse flexible plastic thick film (4), on the plane of operation plate (3) bottom two columns (5) are arranged, in the middle of two columns (5) draw-in groove (6) is arranged, the hole (7) that is connected with base plate (1) is arranged in the draw-in groove (6).
2. neonatal rat as claimed in claim 1 is got the brain fixture, it is characterized in that, described base plate (1) is made by rustless steel.
3. neonatal rat as claimed in claim 1 is got the brain fixture, it is characterized in that, described support (2) is made by rustless steel.
4. neonatal rat as claimed in claim 1 is got the brain fixture, it is characterized in that, described operation plate (3) is made by the good tempered glass of light transmission.
5. neonatal rat as claimed in claim 1 is got the brain fixture, it is characterized in that, thick film (4) and column (5) that described operation plate bottom covers are made by flexible plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210548595.5A CN103006347B (en) | 2012-12-17 | 2012-12-17 | Fixing device for suckling mouse brain extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210548595.5A CN103006347B (en) | 2012-12-17 | 2012-12-17 | Fixing device for suckling mouse brain extraction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103006347A true CN103006347A (en) | 2013-04-03 |
| CN103006347B CN103006347B (en) | 2015-06-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210548595.5A Expired - Fee Related CN103006347B (en) | 2012-12-17 | 2012-12-17 | Fixing device for suckling mouse brain extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103006347B (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001128998A (en) * | 1999-11-08 | 2001-05-15 | Fujihira Industry Co Ltd | Fixing device for craniotomy of domestic animal |
| JP2003190191A (en) * | 2001-12-28 | 2003-07-08 | Oriental Yeast Co Ltd | Animal fixation implement |
| CN2707211Y (en) * | 2004-05-20 | 2005-07-06 | 中国人民解放军第三军医大学 | Brain fixing device for lucid small sized animal without muscle relaxation inducing condition |
| CN2808086Y (en) * | 2005-02-07 | 2006-08-23 | 首都医科大学北京神经科学研究所 | Fixing apparatus suitable for sober animal cephalosome operation |
| CN201055438Y (en) * | 2007-06-28 | 2008-05-07 | 北京生命科学研究所 | Animal head fixing device with two-dimension regulation function |
| JP2008200007A (en) * | 2007-02-22 | 2008-09-04 | Institute Of Physical & Chemical Research | Device of animal experiment |
| CN201260706Y (en) * | 2008-09-10 | 2009-06-24 | 深圳大学 | Multifunctional animal dissecting table for experiment |
| CN201328892Y (en) * | 2009-01-05 | 2009-10-21 | 中国人民解放军第四军医大学 | Rat fixture unit for medicine experiment |
| CN201692092U (en) * | 2010-03-29 | 2011-01-05 | 刘辉 | Mouse fixer |
| CN201847793U (en) * | 2010-06-01 | 2011-06-01 | 浙江省医学科学院 | Soft material rat fixing device |
| CN201977959U (en) * | 2011-03-04 | 2011-09-21 | 苏州大学 | Mice fixing device for irradiation experiment |
| CN202010216U (en) * | 2011-03-16 | 2011-10-19 | 佳木斯大学 | Handheld cavy blood collecting fixer |
| CN202458771U (en) * | 2012-02-27 | 2012-10-03 | 河南农业大学 | Mouse experimental fixing device |
-
2012
- 2012-12-17 CN CN201210548595.5A patent/CN103006347B/en not_active Expired - Fee Related
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001128998A (en) * | 1999-11-08 | 2001-05-15 | Fujihira Industry Co Ltd | Fixing device for craniotomy of domestic animal |
| JP2003190191A (en) * | 2001-12-28 | 2003-07-08 | Oriental Yeast Co Ltd | Animal fixation implement |
| CN2707211Y (en) * | 2004-05-20 | 2005-07-06 | 中国人民解放军第三军医大学 | Brain fixing device for lucid small sized animal without muscle relaxation inducing condition |
| CN2808086Y (en) * | 2005-02-07 | 2006-08-23 | 首都医科大学北京神经科学研究所 | Fixing apparatus suitable for sober animal cephalosome operation |
| JP2008200007A (en) * | 2007-02-22 | 2008-09-04 | Institute Of Physical & Chemical Research | Device of animal experiment |
| CN201055438Y (en) * | 2007-06-28 | 2008-05-07 | 北京生命科学研究所 | Animal head fixing device with two-dimension regulation function |
| CN201260706Y (en) * | 2008-09-10 | 2009-06-24 | 深圳大学 | Multifunctional animal dissecting table for experiment |
| CN201328892Y (en) * | 2009-01-05 | 2009-10-21 | 中国人民解放军第四军医大学 | Rat fixture unit for medicine experiment |
| CN201692092U (en) * | 2010-03-29 | 2011-01-05 | 刘辉 | Mouse fixer |
| CN201847793U (en) * | 2010-06-01 | 2011-06-01 | 浙江省医学科学院 | Soft material rat fixing device |
| CN201977959U (en) * | 2011-03-04 | 2011-09-21 | 苏州大学 | Mice fixing device for irradiation experiment |
| CN202010216U (en) * | 2011-03-16 | 2011-10-19 | 佳木斯大学 | Handheld cavy blood collecting fixer |
| CN202458771U (en) * | 2012-02-27 | 2012-10-03 | 河南农业大学 | Mouse experimental fixing device |
Non-Patent Citations (2)
| Title |
|---|
| 刘保新等: "《介绍一种简易的大鼠固定装置》", 《中国比较医学杂志》 * |
| 刘文忠等: "《一种简易的大鼠固定装置》", 《实验动物科学与管理》 * |
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| Publication number | Publication date |
|---|---|
| CN103006347B (en) | 2015-06-03 |
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Granted publication date: 20150603 Termination date: 20171217 |