CN105167875A - Small animal operating table - Google Patents
Small animal operating table Download PDFInfo
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- CN105167875A CN105167875A CN201510583093.XA CN201510583093A CN105167875A CN 105167875 A CN105167875 A CN 105167875A CN 201510583093 A CN201510583093 A CN 201510583093A CN 105167875 A CN105167875 A CN 105167875A
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- operating
- meiofauna
- cavity
- waste collection
- operation panel
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Abstract
The invention discloses a small animal operating table which comprises a cavity with multiple functional cavity bodies and multiple working plates covering the cavity. Each functional cavity body comprises a heating medium cavity body, a refrigerant cavity body and a waste liquid collecting cavity body; each working plate comprises a heating operating plate body arranging on the heating medium cavity body in a covering mode, a refrigerating operating plate body arranging on the refrigerant cavity body in a covering mode and an operation operating plate body arranging on the waste liquid collecting cavity body in a covering mode. According to the small animal operating table, scattered laboratory equipment is integrated into a whole, and not only is the space saved, but also the animal operation quality is greatly improved.
Description
Technical field
The present invention relates to a kind of biomedicine experiment instrument, particularly relate to a kind of meiofauna operating-table.
Background technology
At present, the effective means of whole end-stage liver disease such as treatment hepatitis gravis, liver failure etc. is still liver transplantation, but supplies liver source anxiety, somewhat expensive and there is the problems such as immunological rejection all seriously to limit liver transplantation in clinical extensive use.
Bioartificial liver's technology emerging is in recent years expected to solve an above-mentioned difficult problem, can realize the reparation of liver function, substitute and rebuild.But bioartificial liver's technology also has many difficult problems in implementation process, as the seed cell source deficiency in people liver source, limited amount, cell function can not effectively maintain in vitro, the most important function of detoxification of liver can not have efficient recovery etc.For above problem, research has in recent years had more considerable breakthrough.Such as, utilizing different culture environment, timbering material etc. to reappear as far as possible makes cell function promote and long term maintenance in the hepatocellular living environment of body; Use the seed cell of hepatocyte replacement as bioartificial liver's process of animal origin, and along with a series of research how obtaining the primary animal liver cell extracting method that quantity is large and vigor is high; And the continuous improvement of animal experiment device.It is more and more feasible that these all make bioartificial liver be generalized to clinical target.
But the method for the animal of each laboratory extraction at present primary hepatocyte varies, and the equipment of use is also not quite similar.Particularly when carrying out operation digestion to animal and extracting hepatocyte, because extraction step is loaded down with trivial details and have many special extraction conditions to limit, make to complete in same place to extracting great-hearted hepatocellular whole process from anesthetized animal, and need to toss about in bed between different devices.Which not only adds the contaminated probability of the cell extracted, vigor declines even dead due to long-time ischemia also to make cell in leaching process.In addition, the work efficiency of operator is also difficult to further raising.
Existing experimental facilities is not specifically designed to the complete set equipment that animal (mice) primary hepatocyte extracts, but the equipment of various necessity spreads out, and is unfavorable for the operation carrying out linking up and at substantial time and manpower.
Further, because different action needs transfers to different operating equipment places, make operating time prolongation, the increase of cell contamination probability, also make the Cell viability extracted reduce.
Summary of the invention
The object of the invention is the convenience implementing operation for improving minitype animal experiment, ensureing surgical quality, a kind of meiofauna operating-table with comprehensive function is provided.
For reaching above technical purpose, the technical solution used in the present invention is as follows:
A kind of meiofauna operating-table, it comprises the cavity that is provided with multiple functional chamber and covers the multiple work plate bodys on described cavity; Described functional chamber comprises heat medium cavity, coolant chamber and waste collection chamber; Described work plate body comprises the hot operation panel covered above described heat medium cavity, the operation technique plate covering the cold operation panel above described coolant chamber and cover above described waste collection chamber.
Preferably, described heat medium cavity inside is provided with electroluminescent electro-heat equipment.Preferably, described electroluminescent electro-heat equipment is thermostatic heater.
Selectively, described coolant intracavity portion is provided with electroluminescent refrigerating plant.
More preferably, described waste collection intracavity portion is provided with liquid-collecting box.
For ease of dismounting with replace, described hot operation panel and removably covering respectively between cold operation panel and described cavity.
For ease of fixing various container, described hot operation panel and cold operation panel offer in culture dish holddown groove, fluid infusion bottle fixing hole and centrifuge tube fixing hole one of at least.
Automatically discharge for ease of waste liquid, the difference in height between described operation technique plate and cavity bottom is successively decreased from the top edge of this operation technique plate to lower limb.Further, described operation technique plate is offering osculum near its lower edge; Described waste collection chamber is corresponding with described osculum to be arranged.
For ease of heating described operation technique plate, described heat medium cavity and waste collection chamber vicinity, described operation technique plate covers whole described waste collection chamber and the described heat medium cavity of part.
Preferably, described operation technique plate is provided with the clip of fixing animal head and/or extremity.
Compared with prior art, the present invention has following advantage:
(1) meiofauna operating-table of the present invention, integrates scattered laboratory equlpment, reduces the volume of equipment, and the equipment of being convenient to is placed in superclean bench and carries out aseptic operation operation;
(2) meiofauna operating-table of the present invention, makes operation technique can complete in a place, has not only saved the operating time, it also avoid because needing to come and go the pollution caused between different devices;
(3) the hot operation panel in meiofauna operating-table of the present invention and cold operation panel not only can be dismantled, change, and the various holddown groove that it is offered and fixing hole can also customize;
(4) operating-table in meiofauna operating-table of the present invention is provided with the weathering had a down dip, and can automatically drain the waste liquid produced in operation process, is convenient to the lasting enforcement of performing the operation;
(5) liquid-collecting box of meiofauna operating-table of the present invention can take out and clean from waste collection chamber, facilitates the complete equipment cleaning of operation and maintenance;
(6) meiofauna operating-table of the present invention, not only may be used for carrying out hepatocyte extraction to mice as the above-mentioned, can also be applied in the operation technique of other meiofaunas (as rat, rabbit, the frog, Cavia porcellus etc.), therefore, this equipment has broad application prospects.
Accompanying drawing explanation
Fig. 1 is that the master of meiofauna operating-table of the present invention looks perspective view.
Fig. 2 is the stereochemical structure decomposing schematic representation of meiofauna operating-table of the present invention.
Fig. 3 is that perspective view is looked on the right side of meiofauna operating-table of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
With reference to figure 1 and Fig. 2, meiofauna operating-table of the present invention comprises the cavity 1 that is provided with multiple functional chamber and covers the work plate body 2 on this cavity 1.
As shown in Figure 2, described cavity 1 comprises heat medium cavity 11, is arranged in the coolant chamber 12 of heat medium cavity 11 lower left corner part and is arranged in the waste collection chamber 13 of this heat medium cavity 11 lower right corner part.
Further, described heat medium cavity 11 inside is provided with by heating bar 1111 and the thermostatic heater 111 that forms with the attemperating unit 1112 that this heating bar 1111 is electrically connected.Thus, the function class of this heat medium cavity 11, like water-bath, can provide treatment temperature higher than room temperature for zooblast, tissue or organ; Selectively, this heat medium cavity 11 also can arrange the thermostatic heater such as electroluminescent electro-heat equipment of other types according to actual needs.
Described coolant chamber 12 is cavity structure, for placing trash ice, can provide the temperature of about 0 DEG C thus for zooblast, tissue or organ; Selectively, this coolant chamber 12 also can arrange electroluminescent refrigerating plant according to actual needs for providing the treatment temperature lower than room temperature.
The lower end in described waste collection chamber 13 forms opening (non-label), to be inserted by liquid-collecting box 131 in this waste collection chamber 13 by this opening, or takes out from waste collection chamber 13.The side of described liquid-collecting box 131 can arrange handle, so that liquid-collecting box 131 described in push-and-pull.
As shown in Figure 1, described work plate body 2 comprises the hot operation panel 21 covered above described heat medium cavity 11, the operation technique plate 23 covering the cold operation panel 22 above described coolant chamber 12 and cover above described waste collection chamber 13.
Removably cover between described hot operation panel 21 and described heat medium cavity 11, this hot operation panel 21 offers in culture dish holddown groove a, fluid infusion bottle fixing hole b and centrifuge tube fixing hole c one of at least.Described culture dish holddown groove a, fluid infusion bottle fixing hole b and the quantity of centrifuge tube fixing hole c, respective size and position can need to arrange according to animal surgery, or often kind of described holddown groove (hole) is all provided with several at ad-hoc location, operator need to select according to animal surgery.
Similarly, also be removably cover between described cold operation panel 22 and described coolant chamber 12, and this cold operation panel 22 also offers in culture dish holddown groove a, fluid infusion bottle fixing hole b and centrifuge tube fixing hole c one of at least, described culture dish holddown groove a, fluid infusion bottle fixing hole b are consistent with the arrangement of centrifuge tube fixing hole c with the culture dish holddown groove a on described hot operation panel 21, fluid infusion bottle fixing hole b with the arrangement of centrifuge tube fixing hole c.
With reference to figure 1 and Fig. 3, preferably cover to be fixedly connected with mode between described operation technique plate 13 with described waste collection chamber 13, this operation technique plate 13 is provided with multiple clip 231 for fixing animal head and extremity, and be provided with meniscate osculum 232 at this operation technique plate 13 near its lower edge, this osculum 232 is just to waste collection chamber 13 or the liquid-collecting box 131 of below.
Preferably, waste liquid for ease of operation process generation can be drained automatically does not affect operation technique, difference in height between this operation technique plate 23 and bottom of described cavity 1 successively decreases to form weathering from the top edge of this operation technique plate 23 to lower limb, forms the weathering (as shown in Figure 3) with angle [alpha] between that is bottom this operation technique plate 23 and described cavity 1.
Further, continue with reference to figure 2, for keeping the vigor of each cell of animal, tissue or organ in operation process, need to carry out suitable heated at constant temperature to described operation technique plate 23, therefore, described heat medium cavity 11 and waste collection chamber 13 are disposed adjacent, and, by the position that the first half that the scope of described heat medium cavity 11 extends to described operation technique plate 23 can cover, therefore, described waste collection chamber 13 is covered by the latter half of described operation technique plate 23.Thus, described operation technique plate 23 has covered the heat medium cavity 11 in whole described waste collection chamber 13 and part; When described heat medium cavity is full of the hot water of constant temperature, hot water not only can to described hot operation panel 21 transferring heat, simultaneously also to described operation technique plate transferring heat.
Further, for avoiding producing exchange heat between described heat medium cavity 11 and coolant chamber 12, thus respective temperature cannot be maintained for a long time, the chamber wall in described heat medium cavity 11 and coolant chamber 12 is respectively equipped with thermal insulation layer; Or, isolated with other functional chamber (as waste collection chamber) between described heat medium cavity 11 and coolant chamber 12.
Below to get the general collagenase circumfusion method of mouse primary hepatocytes at present to the using method of this meiofauna operating-table of explaining in detail.
1, the preparation of operating-table and other articles for use:
Infusion liquid before configuring is loaded in 100ml vial (or fluid infusion bottle), with masking foil sealing and autoclaving; Bottle stopper and silica gel tube autoclaving together with operating theater instruments.Rear infusion liquid filtration sterilization loads 50ml centrifuge tube.50ml centrifuge tube is loaded after stop buffer aseptic process.Described meiofauna operating-table is put into superclean bench and is carried out disinfection by ultraviolet light.
Animal (mice) prepares properly, then pour into described heat medium cavity 11 with water and heat, trash ice being put into described coolant chamber 12; Now, sterilized front infusion liquid and rear infusion liquid are all put on described hot operation panel 21 and are carried out preheating, and wherein, described front infusion liquid puts into described fluid infusion bottle fixing hole b, and described rear infusion liquid puts into described centrifuge tube fixing hole C.The centrifuge tube fixing hole C that described stop buffer is put on described cold operation panel 22 carries out pre-cooling.
2, animal surgery:
Be fixed on described operation technique plate 23 on described clip 231 by mice by after mouse anesthesia, the success of operation technique portal catheterization also fixes remaining needle; Utilize front infusion liquid vial described in a termination of silica gel tube, another termination remaining needle, middle peristaltic pump of crossing is for perfusion.
Pour into separating mouse liver successfully, be placed into 6cm sterile petri dish (culture dish is placed in the culture dish holddown groove a of hot operation panel 21), import the described rear infusion liquid of preheating, remaining needle described in the termination recycling another silica gel tube, the other end is dipped in the rear infusion liquid of described culture dish, and middle peristaltic pump of crossing is for perfusion.
After having poured into, import the stop buffer cessation reaction of described pre-cooling; Then described culture dish is transferred on the culture dish holddown groove a of described cold operation panel 22.Tear liver tunicle release hepatocyte, cell suspension in culture dish is filled in the 50ml centrifuge tube (pre-cooling in described cold operation panel 22) of pre-cooling, filters twice.After this carry out low-temperature centrifugation and cell is resuspended all carries out on described cold operation panel 22, the waste liquid produced in process can directly be poured in described waste collection chamber 13 or liquid-collecting box 131.
In sum, scattered laboratory equlpment integrates by meiofauna operating-table of the present invention, not only saves space, and greatly improves animal surgery quality.
Above-described embodiment is the present invention's preferably embodiment; but be not merely restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be all included within protection scope of the present invention.
Claims (11)
1. a meiofauna operating-table, is characterized in that: it comprises the cavity that is provided with multiple functional chamber and covers the multiple work plate bodys on described cavity; Described functional chamber comprises heat medium cavity, coolant chamber and waste collection chamber; Described work plate body comprises the hot operation panel covered above described heat medium cavity, the operation technique plate covering the cold operation panel above described coolant chamber and cover above described waste collection chamber.
2. meiofauna operating-table as claimed in claim 1, is characterized in that: described heat medium cavity inside is provided with electroluminescent electro-heat equipment.
3. meiofauna operating-table as claimed in claim 2, is characterized in that: described electroluminescent electro-heat equipment is thermostatic heater.
4. meiofauna operating-table as claimed in claim 1, is characterized in that: described coolant intracavity portion is provided with electroluminescent refrigerating plant.
5. meiofauna operating-table as claimed in claim 1, is characterized in that: described waste collection intracavity portion is provided with liquid-collecting box.
6. meiofauna operating-table as claimed in claim 1, is characterized in that: described hot operation panel and removably covering respectively between cold operation panel and described cavity.
7. meiofauna operating-table as claimed in claim 6, is characterized in that: described hot operation panel and cold operation panel all offer in culture dish holddown groove, fluid infusion bottle fixing hole and centrifuge tube fixing hole one of at least.
8. meiofauna operating-table as claimed in claim 1, is characterized in that: the difference in height between described operation technique plate and cavity bottom is successively decreased from the top edge of this operation technique plate to lower limb.
9. meiofauna operating-table as claimed in claim 8, is characterized in that: described operation technique plate is offering osculum near its lower edge; Described waste collection chamber is corresponding with described osculum to be arranged.
10. the meiofauna operating-table as described in claim 1 or 9, is characterized in that: described heat medium cavity and waste collection chamber vicinity, described operation technique plate covers whole described waste collection chamber and the described heat medium cavity of part.
11. meiofauna operating-tables as claimed in claim 1, is characterized in that: described operation technique plate is provided with the clip of fixing animal head and/or extremity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510583093.XA CN105167875B (en) | 2015-09-14 | 2015-09-14 | Meiofauna operating-table |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510583093.XA CN105167875B (en) | 2015-09-14 | 2015-09-14 | Meiofauna operating-table |
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| Publication Number | Publication Date |
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| CN105167875A true CN105167875A (en) | 2015-12-23 |
| CN105167875B CN105167875B (en) | 2017-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510583093.XA Active CN105167875B (en) | 2015-09-14 | 2015-09-14 | Meiofauna operating-table |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106859805A (en) * | 2017-02-23 | 2017-06-20 | 中国人民解放军第二军医大学 | Portable integrated animal experiment operating table |
| CN108514462A (en) * | 2018-06-11 | 2018-09-11 | 新乡医学院 | A kind of mouse perfusion fixing device and its perfusion fixing means |
| CN111304075A (en) * | 2020-03-09 | 2020-06-19 | 复旦大学附属中山医院 | Adult mouse myocardium separator |
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| CN2080393U (en) * | 1990-12-30 | 1991-07-10 | 泰山医学院 | Controller for medical constant temp. mattress capable of controlling temp. |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106859805A (en) * | 2017-02-23 | 2017-06-20 | 中国人民解放军第二军医大学 | Portable integrated animal experiment operating table |
| CN108514462A (en) * | 2018-06-11 | 2018-09-11 | 新乡医学院 | A kind of mouse perfusion fixing device and its perfusion fixing means |
| CN111304075A (en) * | 2020-03-09 | 2020-06-19 | 复旦大学附属中山医院 | Adult mouse myocardium separator |
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| Publication number | Publication date |
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| CN105167875B (en) | 2017-04-05 |
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