CN103005165A - Preparation method of lactobionic acid microelement complex and application of complex as animal feed additive - Google Patents
Preparation method of lactobionic acid microelement complex and application of complex as animal feed additive Download PDFInfo
- Publication number
- CN103005165A CN103005165A CN201310006335XA CN201310006335A CN103005165A CN 103005165 A CN103005165 A CN 103005165A CN 201310006335X A CN201310006335X A CN 201310006335XA CN 201310006335 A CN201310006335 A CN 201310006335A CN 103005165 A CN103005165 A CN 103005165A
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- China
- Prior art keywords
- lactobionic acid
- feed
- counted
- zinc
- per ton
- Prior art date
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- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 title claims abstract description 264
- 229940099563 lactobionic acid Drugs 0.000 title claims abstract description 258
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- 235000019730 animal feed additive Nutrition 0.000 title description 6
- 241001465754 Metazoa Species 0.000 claims abstract description 57
- 238000006243 chemical reaction Methods 0.000 claims abstract description 52
- 239000011573 trace mineral Substances 0.000 claims abstract description 43
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 43
- 241000287828 Gallus gallus Species 0.000 claims abstract description 39
- 150000001875 compounds Chemical class 0.000 claims abstract description 33
- 230000012010 growth Effects 0.000 claims abstract description 30
- 238000003756 stirring Methods 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 241000272814 Anser sp. Species 0.000 claims abstract description 19
- 244000144972 livestock Species 0.000 claims abstract description 13
- 238000001816 cooling Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000003960 organic solvent Substances 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
- 239000012670 alkaline solution Substances 0.000 claims abstract description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 95
- 239000011701 zinc Substances 0.000 claims description 56
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 54
- 229910052725 zinc Inorganic materials 0.000 claims description 54
- 239000010949 copper Substances 0.000 claims description 51
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 50
- 229910052802 copper Inorganic materials 0.000 claims description 50
- 229910052759 nickel Inorganic materials 0.000 claims description 47
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 44
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 43
- 239000010941 cobalt Substances 0.000 claims description 43
- 229910017052 cobalt Inorganic materials 0.000 claims description 43
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 43
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 37
- 239000011651 chromium Substances 0.000 claims description 37
- 229910052804 chromium Inorganic materials 0.000 claims description 36
- 239000011572 manganese Substances 0.000 claims description 35
- 229910052748 manganese Inorganic materials 0.000 claims description 34
- 229910052732 germanium Inorganic materials 0.000 claims description 32
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 claims description 32
- 241000272525 Anas platyrhynchos Species 0.000 claims description 22
- 241000282849 Ruminantia Species 0.000 claims description 21
- 238000002425 crystallisation Methods 0.000 claims description 19
- 230000008025 crystallization Effects 0.000 claims description 19
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 15
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 claims description 14
- 241000252233 Cyprinus carpio Species 0.000 claims description 10
- 244000309466 calf Species 0.000 claims description 10
- -1 manganese element compound Chemical class 0.000 claims description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 10
- 244000144987 brood Species 0.000 claims description 9
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 8
- 230000036039 immunity Effects 0.000 claims description 7
- 230000001850 reproductive effect Effects 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 230000011132 hemopoiesis Effects 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000011592 zinc chloride Substances 0.000 claims description 5
- 235000005074 zinc chloride Nutrition 0.000 claims description 5
- 239000005749 Copper compound Substances 0.000 claims description 4
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims description 4
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 4
- 150000001880 copper compounds Chemical class 0.000 claims description 4
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 4
- YBMRDBCBODYGJE-UHFFFAOYSA-N germanium oxide Inorganic materials O=[Ge]=O YBMRDBCBODYGJE-UHFFFAOYSA-N 0.000 claims description 4
- QWTDNUCVQCZILF-UHFFFAOYSA-N isopentane Chemical compound CCC(C)C QWTDNUCVQCZILF-UHFFFAOYSA-N 0.000 claims description 4
- 229940099596 manganese sulfate Drugs 0.000 claims description 4
- 239000011702 manganese sulphate Substances 0.000 claims description 4
- 235000007079 manganese sulphate Nutrition 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 229910000008 nickel(II) carbonate Inorganic materials 0.000 claims description 4
- ZULUUIKRFGGGTL-UHFFFAOYSA-L nickel(ii) carbonate Chemical compound [Ni+2].[O-]C([O-])=O ZULUUIKRFGGGTL-UHFFFAOYSA-L 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 claims description 3
- 235000002867 manganese chloride Nutrition 0.000 claims description 3
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 claims description 3
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- 229910021556 Chromium(III) chloride Inorganic materials 0.000 claims description 2
- 229910021503 Cobalt(II) hydroxide Inorganic materials 0.000 claims description 2
- 239000004277 Ferrous carbonate Substances 0.000 claims description 2
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 2
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 claims description 2
- YPWFISCTZQNZAU-UHFFFAOYSA-N Thiane Chemical compound C1CCSCC1 YPWFISCTZQNZAU-UHFFFAOYSA-N 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 229960000359 chromic chloride Drugs 0.000 claims description 2
- GRWVQDDAKZFPFI-UHFFFAOYSA-H chromium(III) sulfate Chemical compound [Cr+3].[Cr+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O GRWVQDDAKZFPFI-UHFFFAOYSA-H 0.000 claims description 2
- VQWFNAGFNGABOH-UHFFFAOYSA-K chromium(iii) hydroxide Chemical compound [OH-].[OH-].[OH-].[Cr+3] VQWFNAGFNGABOH-UHFFFAOYSA-K 0.000 claims description 2
- 229910021446 cobalt carbonate Inorganic materials 0.000 claims description 2
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000428 cobalt oxide Inorganic materials 0.000 claims description 2
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 claims description 2
- ZOTKGJBKKKVBJZ-UHFFFAOYSA-L cobalt(2+);carbonate Chemical compound [Co+2].[O-]C([O-])=O ZOTKGJBKKKVBJZ-UHFFFAOYSA-L 0.000 claims description 2
- HIYNGBUQYVBFLA-UHFFFAOYSA-D cobalt(2+);dicarbonate;hexahydroxide Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Co+2].[Co+2].[Co+2].[Co+2].[Co+2].[O-]C([O-])=O.[O-]C([O-])=O HIYNGBUQYVBFLA-UHFFFAOYSA-D 0.000 claims description 2
- 229910000001 cobalt(II) carbonate Inorganic materials 0.000 claims description 2
- ASKVAEGIVYSGNY-UHFFFAOYSA-L cobalt(ii) hydroxide Chemical compound [OH-].[OH-].[Co+2] ASKVAEGIVYSGNY-UHFFFAOYSA-L 0.000 claims description 2
- IVMYJDGYRUAWML-UHFFFAOYSA-N cobalt(ii) oxide Chemical compound [Co]=O IVMYJDGYRUAWML-UHFFFAOYSA-N 0.000 claims description 2
- 229940116318 copper carbonate Drugs 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- GEZOTWYUIKXWOA-UHFFFAOYSA-L copper;carbonate Chemical compound [Cu+2].[O-]C([O-])=O GEZOTWYUIKXWOA-UHFFFAOYSA-L 0.000 claims description 2
- AEJIMXVJZFYIHN-UHFFFAOYSA-N copper;dihydrate Chemical compound O.O.[Cu] AEJIMXVJZFYIHN-UHFFFAOYSA-N 0.000 claims description 2
- 229960004643 cupric oxide Drugs 0.000 claims description 2
- AFABGHUZZDYHJO-UHFFFAOYSA-N dimethyl butane Natural products CCCC(C)C AFABGHUZZDYHJO-UHFFFAOYSA-N 0.000 claims description 2
- FNIHDXPFFIOGKL-UHFFFAOYSA-N disodium;dioxido(oxo)germane Chemical compound [Na+].[Na+].[O-][Ge]([O-])=O FNIHDXPFFIOGKL-UHFFFAOYSA-N 0.000 claims description 2
- 235000019268 ferrous carbonate Nutrition 0.000 claims description 2
- RAQDACVRFCEPDA-UHFFFAOYSA-L ferrous carbonate Chemical compound [Fe+2].[O-]C([O-])=O RAQDACVRFCEPDA-UHFFFAOYSA-L 0.000 claims description 2
- 229960004652 ferrous carbonate Drugs 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000015 iron(II) carbonate Inorganic materials 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 239000011565 manganese chloride Substances 0.000 claims description 2
- IPJKJLXEVHOKSE-UHFFFAOYSA-L manganese dihydroxide Chemical compound [OH-].[OH-].[Mn+2] IPJKJLXEVHOKSE-UHFFFAOYSA-L 0.000 claims description 2
- PPNAOCWZXJOHFK-UHFFFAOYSA-N manganese(2+);oxygen(2-) Chemical compound [O-2].[Mn+2] PPNAOCWZXJOHFK-UHFFFAOYSA-N 0.000 claims description 2
- VASIZKWUTCETSD-UHFFFAOYSA-N manganese(II) oxide Inorganic materials [Mn]=O VASIZKWUTCETSD-UHFFFAOYSA-N 0.000 claims description 2
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 2
- 229910000480 nickel oxide Inorganic materials 0.000 claims description 2
- BFDHFSHZJLFAMC-UHFFFAOYSA-L nickel(ii) hydroxide Chemical compound [OH-].[OH-].[Ni+2] BFDHFSHZJLFAMC-UHFFFAOYSA-L 0.000 claims description 2
- PVADDRMAFCOOPC-UHFFFAOYSA-N oxogermanium Chemical compound [Ge]=O PVADDRMAFCOOPC-UHFFFAOYSA-N 0.000 claims description 2
- GNRSAWUEBMWBQH-UHFFFAOYSA-N oxonickel Chemical compound [Ni]=O GNRSAWUEBMWBQH-UHFFFAOYSA-N 0.000 claims description 2
- RGSFGYAAUTVSQA-UHFFFAOYSA-N pentamethylene Natural products C1CCCC1 RGSFGYAAUTVSQA-UHFFFAOYSA-N 0.000 claims description 2
- 125000004817 pentamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims description 2
- UOURRHZRLGCVDA-UHFFFAOYSA-D pentazinc;dicarbonate;hexahydroxide Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Zn+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2].[O-]C([O-])=O.[O-]C([O-])=O UOURRHZRLGCVDA-UHFFFAOYSA-D 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- LITQZINTSYBKIU-UHFFFAOYSA-F tetracopper;hexahydroxide;sulfate Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Cu+2].[Cu+2].[Cu+2].[Cu+2].[O-]S([O-])(=O)=O LITQZINTSYBKIU-UHFFFAOYSA-F 0.000 claims description 2
- 229960001939 zinc chloride Drugs 0.000 claims description 2
- UGZADUVQMDAIAO-UHFFFAOYSA-L zinc hydroxide Chemical compound [OH-].[OH-].[Zn+2] UGZADUVQMDAIAO-UHFFFAOYSA-L 0.000 claims description 2
- 229910021511 zinc hydroxide Inorganic materials 0.000 claims description 2
- 229940007718 zinc hydroxide Drugs 0.000 claims description 2
- 239000011787 zinc oxide Substances 0.000 claims description 2
- 229960001296 zinc oxide Drugs 0.000 claims description 2
- NDKWCCLKSWNDBG-UHFFFAOYSA-N zinc;dioxido(dioxo)chromium Chemical compound [Zn+2].[O-][Cr]([O-])(=O)=O NDKWCCLKSWNDBG-UHFFFAOYSA-N 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 17
- 241000282887 Suidae Species 0.000 abstract description 11
- 238000001035 drying Methods 0.000 abstract description 6
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- 241000272517 Anseriformes Species 0.000 abstract 1
- 239000003929 acidic solution Substances 0.000 abstract 1
- 239000003674 animal food additive Substances 0.000 abstract 1
- 239000011343 solid material Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 63
- 239000000047 product Substances 0.000 description 53
- 230000002354 daily effect Effects 0.000 description 35
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 239000002994 raw material Substances 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 11
- 229910052742 iron Inorganic materials 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 230000007812 deficiency Effects 0.000 description 8
- 238000004451 qualitative analysis Methods 0.000 description 8
- 238000011084 recovery Methods 0.000 description 8
- 238000010792 warming Methods 0.000 description 8
- 102000001554 Hemoglobins Human genes 0.000 description 7
- 108010054147 Hemoglobins Proteins 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- 208000005156 Dehydration Diseases 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 230000018044 dehydration Effects 0.000 description 6
- 238000006297 dehydration reaction Methods 0.000 description 6
- 235000019197 fats Nutrition 0.000 description 6
- 235000021050 feed intake Nutrition 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 206010022971 Iron Deficiencies Diseases 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 5
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000005352 clarification Methods 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 238000004886 process control Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 210000001541 thymus gland Anatomy 0.000 description 4
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- 241000784728 Lycaena virgaureae Species 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- 208000007502 anemia Diseases 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
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Abstract
The invention discloses a preparation method of a lactobionic acid microelement complex, which comprises the following steps of: adding an inorganic trace element compound and lactobionic acid into water, raising the temperature and then fully stirring for reacting; adding an acidic solution or adding an alkaline solution for fully controlling the pH value of a reaction system; after the reaction is ended, cooling, slowly adding the reaction system into an organic solvent for crystallizing; and then carrying out solid-liquid separation, and drying the separated solid material to obtain the lactobionic acid microelement complex. The lactobionic acid microelement complex prepared by adopting the preparation method can be used as a feed additive to be applied to pigs, ducks, broilers, gooses, aquatic livestock, cud chewers, pets or special economic animals. The lactobionic acid microelement complex has the advantages of simple process, less energy consumption, low cost, environment friendliness, no pollution and the like, is low in adding quantity, remarkable in growth promoting effect, and less in side effects of animals during application, and can be used for a long time.
Description
Technical field
The invention belongs to preparation and the application of animal feed additive, relate in particular to the preparation method of a kind of lactobionic acid trace element complex and as the application of animal feed additive.
Background technology
Lactobionic acid is that the C-1 hydroxyl of glucityl in the lactose molecule is oxidized to the product that obtains behind the carboxyl.Lactobionic acid approval by FDA and be used as curing agent and be used for adding the pudding pre-composition or be used for cosmetics as wetting agent in the U.S..In the structure of lactobionic acid with eight hydroxys, thereby have powerful antioxidation, can avoid cell membrane to be subject to Oxidative demage, improve the reproductive performance of animal and prolong the boar time limit.Lactobionic acid has the effect of accelerating cell metabolism equally; Promote skin corium matrix and collagen to generate, and have the effectiveness of accelerating epithelium formation and accelerating wound healing; Make the red hair of animal skins bright.In addition, lactobionic acid can also the selective stimulating Bifidobacterium activity of propagation, stimulate mineral absorption.
Trace copper can effective participation the important physiology courses such as the pigmentation of formation, keratinization and tissue of cellular oxidation, bone and connective tissue of body and the formation of spinal cord myelin, and keep normal cardiac function to have important function to body.Copper also is the essential component of some enzyme systems, and especially the metalloenzyme relevant with cellular oxidation participated in organism metabolism directly.Absorption and the function of copper and iron are closely related, can keep the eubolism of ferro element, are conducive to the synthetic and erythrocytic maturation of hemoglobin; Can also participate in the breeding activity of body by promoting hypothalamus secretion luteinising hormone-releasing hormo.
Trace elements iron is one of essential trace element of animal, is the pith of hemoglobin.Iron is present in the red blood cell of muscle oxygen gas-supplying, or the composition of many enzymes and immune system compound, and animal absorbs required most of iron from food, and is carefully controlling iron content.Iron can participate in transportation and the storage of oxygen.Iron can also promote to grow; Increase is to the resistance of disease; Regulate tissue respiration, prevent fatigue; Consist of ferroheme, the anaemia that prevention and treatment cause because of iron deficiency; Make the red hair of animal skins bright.
Trace element zinc is one of necessary trace element of animal; likened to animal " biological element " always; since the people such as Todd in 1934 confirm first that by rat test zinc is one of necessary trace element of Animal nutrition; people find gradually; zinc is 200 various metals enzymes in the animal body; the part of hormone and insulin; can promote growing and regeneration of body; keep the eubolism of body; promote appetite; maintenance organ and property function normal accelerates Wound healing, the protection skin health; strengthen immunologic mechanism, have great nutritive value at aspects such as improving resistance.
The Major Nutrient physiological action of trace element manganese is as the enzyme activation factor or part in carbohydrate, lipid, protein and cholesterol metabolic.Manganese can participate in the transmission of nervus centralis media and the energy supply of axoneure, to keep the requisite material of brain eubolism function, the phenomenon such as food ration decline, decreased growth, bone are unusual if the animal manganese deficiency can cause, incoordination, slow in reacting and breeding function are unusual.In addition, thyroid synthesizing must have the enzymatic that activates manganese just can finish.Manganese ion also has much relations with chroma of hair, and the chroma of hair light then may contain the manganese abundance, and the intensely dark pool of hair then may manganese deficiency.
Trace element chromium is one of essential trace element of animal, brings into play special role in the glycometabolism of human body and fat metabolism.Animal is extremely low to the absorption rate of inorganic chromium, less than 1%; And animal can reach 10%～25% to the utilization rate of Organic Chromium.The physiological function of chromium is to cooperate with other material of controlling metabolism to work.Reactive compound glycine chromium can strengthen the biological action of insulin, can by the activation phosphoglucomutase accelerate the utilization of glucose in the animal body, and impel conversion of glucose be fat.Chromium can suppress the biosynthesis of cholesterol, reduces serum total cholesterol and triacylglycerol content and increasing high density lipoprotein cholesterol level, improves the lean meat percentage of letting animals feed.Chromium content in nucleoprotein is higher, and research finds that it can promote the synthetic of RNA, and chromium also affects amino acid transhipment in vivo, promotes protein metabolism and grows.In addition, chromium can also be anti-oxidant, improves immunologic function.
Trace element cobalt and Cobastab
12After entering stomach, with " intrinsic factor " combination of parietal cell secretion, to prevent Cobastab
12Destroyed by enteric microorganism, thereby impel its absorption.Cobalt can also the stimulating animal body in the hemopoietic system of marrow, help animal to make red blood cell, distend the blood vessels and rubefaction, prevent anaemia.Thyroxinic synthesizing may also need cobalt, the impact that cobalt energy antagonism iodine deficiency produces.
Microelement nickel is the essential biological element of animal.Nickel participates in the activity of serum calmness and enzyme and ribonucleic acid, can activate the activity of peptase, all includes nickel on the structural stability of hormonal action and large biological molecule and in the general metabolic processes.Nickel has the effect that stimulates hematopoiesis function, can stimulate red blood cell, ferroheme and leucocyte to increase, and domestic animal lacks nickel and can cause deformity and fade.
Trace element germanium has multiple biologically active, particularly synthesizing first the organic germanium compounds with extensive pharmacologically active from Japanese scholars shallow well one man of virtue and ability in 1967---since the Ge-132, the biologically active of germanium compound has caused countries in the world scholars' very big interest.Studies show that germanium can strengthen the body metabolic function by improving iodine and trilute, TA content, and then promotes the reproductive performance of animal; Meanwhile, germanium makes the quantity of red blood cell in the blood (RBC) and hemoglobin (HB) increase the on the other hand formation of stimulating platelet cellulation (being megacaryocyte), thereby hematopoietic stimulation function on the one hand.Studies show that in a large number organic germanium is to have to promote growth of animal, anticancer, anti-oxidant, reducing blood lipid, removing free radical, improve the BA such as immunity.
As from the foregoing, some specific physiologically active and productive life in the animal body can be kept and improve to elemental copper, iron, zinc, manganese, chromium, cobalt, nickel, germanium, plays vital effect for the life of animal.Their intake deficiency can cause the unusual or diseases of animal physiological to some extent.
About how making lactobionic acid and above-mentioned various trace element organically combine the formation complex, mention in the prior art (referring to the CN1917775A Chinese patent literature), yet, mainly provide in the prior art by the method for preparing calcium lactobionate behind the lactose enzymolysis, and calcium lactobionate is used for the application that the laying hen material is strengthened the eggshell effect.The preparation method of existing Lactobionate (being lactobionic acid trace element complex) is consuming time very long, reactions steps is loaded down with trivial details, cost and energy consumption are very high, output is lower, and the lactose enzymolysis that relates to, the steps such as activated-charcoal column of deionized water balance are had relatively high expectations to technological operation, are unfavorable for large-scale industrial production and application.Therefore how to improve the preparation technology of existing lactobionic acid trace element complex, how to improve its using value, to those skilled in the art, significant.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, provide that a kind of technical process is simple, the preparation method of less energy consumption, low, the environment friendly and pollution-free lactobionic acid trace element of cost complex, also provide low, the short long-acting fruit of a kind of addition obviously, the animal side reaction is few, can use for a long time, safety is without the application of the micro-complex of drug-fast lactobionic acid as animal feed additive.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is a kind of preparation method of lactobionic acid trace element complex, may further comprise the steps: inorganic microelement compound and lactobionic acid are added to the water together, abundant stirring reaction after heating up, add acid solution or add the omnidistance pH value of controlling reaction system of alkaline solution, cooling after reaction finishes, reaction system is slowly joined crystallization in the organic solvent, then carry out Separation of Solid and Liquid, solid matter after the separation namely obtains lactobionic acid trace element complex after super-dry, the recyclable organic solvent that obtains in the liquid after the Separation of Solid and Liquid, and the organic solvent that obtains after reclaiming can be reused.
The preparation method of above-mentioned lactobionic acid trace element complex, described inorganic microelement compound preferably includes copper compound, ferro element compound, zinc element compound, manganese element compound, chromium element compound, cobalt element compound, nickel element compound or Ge element compound.
Described copper compound preferably includes at least a in copper chloride, copper sulphate, cupric oxide, Kocide SD, basic copper chloride, basic copper sulfate, the basic copper carbonate.
Described ferro element compound preferably includes at least a in frerrous chloride, ferrous sulfate, the ferrous carbonate.
Described zinc element compound preferably includes at least a in zinc chloride, zinc sulfate, zinc hydroxide, zinc oxide, basic zinc sulfate, basic zinc carbonate, the basic zinc chloride.
Described manganese element compound preferably includes at least a in manganous chloride, manganese sulfate, manganous hydroxide, manganous oxide, the alkali formula manganese sulfate.
Described chromium element compound preferably includes at least a in chromium trichloride, chromium sulfate, chromium hydroxide, the zinc tetroxychromate.
Described cobalt element compound preferably includes at least a in cobalt oxide, cobalt hydroxide, cobalt chloride, cobaltous sulfate, cobalt carbonate, the basic cobaltous carbonate.
Described nickel element compound preferably includes at least a in nickel oxide, nickel hydroxide, nickel chloride, nickelous sulfate, nickelous carbonate, the basic nickel carbonate.
Described Ge element compound preferably includes at least a in germanium oxide, germanous chloride, calcium germinate, germanic acid copper, sodium metagermanate, two sodium germanates, four sodium germanates.
Above-mentioned various inorganic microelement compounds can be for without crystallization water form or include the hydrate forms of the crystallization water.
The preparation method of above-mentioned lactobionic acid trace element complex, in the described inorganic microelement compound molar ratio of metallic element and described lactobionic acid be preferably 1: 0.8～3.5.
The preparation method of above-mentioned lactobionic acid trace element complex, the preferred whole process of pH value of described reaction system is controlled at 4～9.
The preparation method of above-mentioned lactobionic acid trace element complex, the reaction temperature in the described stirring reaction process preferably is controlled at 35 ℃～100 ℃, and the reaction time is preferably 0.5h～3h.
The preparation method of above-mentioned lactobionic acid trace element complex, described organic solvent is preferably at least a in isopropyl ether, isopentane, pentane, benzinum, hexane, thiacyclohexane, the pentamethylene.
The invention described above preparation method directly reacts lactobionic acid and minor metallic element compound, after reaction is finished, cooled reaction system is poured in the organic solvent, forms water-organic solvent system; Owing to contain the lactobionic acid of lactobionic acid trace element complex and trace in the reacted reaction system, the dissolubility difference of these solutes in water-organic solvent system is larger, so rapid crystallization of lactobionic acid trace element complex meeting, the lactobionic acid of trace then remains in aqueous phase, this has just reached the purpose of rapid separation, impurity removal, greatly improve process efficiency, reduced preparation cost.
As a total technical conceive, the present invention also provides the application as feed addictive in pig, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal of lactobionic acid trace element complex that a kind of above-mentioned preparation method makes.Special economic animal is mainly the wild animal of taming successfully but extensively not using aborning among the present invention, such as donkey, bear, masked civet etc.; The animal that pet is mainly under the existence conditions can be domestic, play the company effect, and feeding for the non-economy purpose it.
In the above-mentioned application, described lactobionic acid trace element complex is preferably lactobionic acid copper, lactobionic acid ferrous iron, lactobionic acid zinc, lactobionic acid manganese, lactobionic acid chromium, lactobionic acid cobalt, lactobionic acid nickel or lactobionic acid germanium.
In the above-mentioned application, preferred:
The addition of described lactobionic acid copper in pig feed per ton counted 2ppm～200ppm with copper; The addition of described lactobionic acid copper in the feed of duck per ton, broiler chicken, ruminant or goose counted 0.1ppm～35ppm with copper; Described lactobionic acid copper addition in aquatic animal feed per ton is counted 0.1ppm～25ppm with copper; Described lactobionic acid copper addition in pet per ton or special economic animal feed is counted 0.1ppm～200ppm with copper;
The addition of described lactobionic acid ferrous iron in pig per ton, duck, broiler chicken, goose, ruminant or aquatic livestock counted 20ppm～750ppm with ferrous element; The addition of described lactobionic acid ferrous iron in feed for pet per ton or special economic animal feed counted 10ppm～1250ppm with ferrous element;
The addition of described lactobionic acid zinc in pig feed per ton counted 10ppm～200ppm with the zinc element; The addition of described lactobionic acid zinc in duck per ton, broiler chicken, goose or ruminant feed counted 10ppm～150ppm with the zinc element; The addition of described lactobionic acid zinc in aquatic animal feed per ton counted 5ppm～200ppm with the zinc element; The addition of described lactobionic acid zinc in feed for pet per ton or special economic animal feed counted 5ppm～250ppm with the zinc element;
The addition of described lactobionic acid manganese in pig per ton, duck, broiler chicken, goose, ruminant, pet or special economic animal feed counted 5ppm～150ppm with manganese element; Described lactobionic acid manganese addition in aquatic animal feed per ton is counted 5ppm～100ppm with manganese element;
The addition of described lactobionic acid chromium in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～0.2ppm with the chromium element;
The addition of described lactobionic acid cobalt in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～2ppm with cobalt element;
The addition of described lactobionic acid nickel in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～10ppm with nickel element;
The addition of described lactobionic acid germanium in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～10ppm with Ge element.
Further preferred as above-mentioned application,
The application of described lactobionic acid copper specifically refers to the application as the feed addictive that promotes Russian carp growth in aquatic animal feed of lactobionic acid copper, and the addition of described lactobionic acid copper in Russian carp feed per ton counted 50ppm～200ppm with copper;
The application of described lactobionic acid ferrous iron specifically refers to the application as the feed addictive that promotes the hematopoiesis of growth pig in pig feed of lactobionic acid ferrous iron, and the addition of described lactobionic acid ferrous iron in pig feed per ton counted 100ppm～750ppm with ferrous element;
The application of described lactobionic acid zinc specifically refers to the application as the feed addictive that improves the brood sow reproductive performance in pig feed of lactobionic acid zinc, and the addition of described lactobionic acid zinc in brood sow feed per ton counted 100ppm～200ppm with the zinc element;
The application of described lactobionic acid manganese specifically refers to the application as the feed addictive that improves duckling immunity in the duck feed of lactobionic acid manganese, and the addition of described lactobionic acid manganese in duckling feed per ton counted 50ppm～150ppm with manganese element;
The application of described lactobionic acid chromium specifically refers to the application as the feed addictive that promotes fattening pig fattening in pig feed of lactobionic acid chromium, and the addition of described lactobionic acid chromium in pig feed per ton counted 0.1ppm～0.2ppm with inferior chromium element;
The application of described lactobionic acid cobalt specifically refers to the application as the feed addictive that promotes the calf growth in ruminant feed of lactobionic acid cobalt, and the addition of described lactobionic acid cobalt in calf feed per ton counted 0.2ppm～2ppm with cobalt element;
The application of described lactobionic acid nickel specifically refer to lactobionic acid nickel in the chicken feed as promoting growth of meat chicken, improve the application of the feed addictive of broiler carcass quality that the addition of described lactobionic acid nickel in broiler fodder per ton counted 3ppm～10ppm with nickel element;
The application of described lactobionic acid germanium specifically refers to the application as the feed addictive that promotes the weanling pig growth in pig feed of lactobionic acid germanium, and described lactobionic acid germanium addition in weanling pig feed per ton is counted 3ppm～10ppm with Ge element.
Compared with prior art, the invention has the advantages that:
(1) simple, the less energy consumption of preparation process of the present invention, cost are low, environment friendly and pollution-free, and the quality of the lactobionic acid trace element complex that makes is better;
(2) the lactobionic acid trace element complex that makes of the present invention can with other reaction of composition generation antagonism and redox reaction in the feed, can guarantee the stable, high-quality, efficient of feed; And the lactobionic acid that the present invention makes trace element complex can enter in the body in modes such as pinocytosis, active transport, stimulate little to stomach, promote intestines peristalsis, make body absorb simultaneously and better the elements such as lactobionic acid and copper, iron, zinc, manganese, chromium, cobalt, nickel, germanium;
(3) the lactobionic acid trace element complex that makes of the present invention is in the application as animal feed additive, have low, the short long-acting fruit of addition obviously, the animal side reaction is few, can use for a long time, safety is carried a little without the resistance to the action of a drug etc., can obviously improve the utilization rate of trace element, animal wastes are shaped better, the excrement stink significantly reduces, and copper, iron, zinc, manganese, chromium, cobalt, nickel, Ge element content reduce greatly in the animal wastes, the pollution to environment can be effectively reduced, the environmental protection pressure of plant can be reduced.
The specific embodiment
Below in conjunction with concrete preferred embodiment the present invention is described further, but does not limit protection scope of the present invention because of following specific embodiment.Below the raw material that relates among each embodiment be commercially available feed grade or analyze pure.
Embodiment 1: the preparation of lactobionic acid chromic compound.
In reactor, add 2t water, open and stir, again six hydrated chromium trichlorides and the 1254kg lactobionic acid of 266kg are knocked down respectively (the lactobionic acid residue: the mol ratio of chromium is about 3: 1) in the reactor, be warming up to 80 ℃, abundant stirring reaction 3h, the sodium hydroxide solution that constantly adds mass concentration 5% between the stage of reaction is about 8 with whole process control pH value; Cooling after reaction finishes, again reaction system is slowly joined in the isopropyl ether pond of at the uniform velocity stirring, should control well adding speed with prevent crystallization too fast cause the caking or crystal formation bad, add and stop after complete stirring, leave standstill to upper strata liquid and clarify, the collection supernatant is delivered to the solvent recovery workshop and is carried out the isopropyl ether recovery, and it is reusable to reclaim the isopropyl ether that obtains; Enter expansion drying after wet feed is centrifugal, obtain lactobionic acid chromium 1.09t.
TLC point plate method is adopted in the product purity qualitative analysis that present embodiment makes.A small amount of raw milk saccharic acid and product lactobionic acid trace element complex are used respectively the methyl alcohol dissolve complete; A small amount of iodine grain is put into the wide-mouth bottle with cover that silica gel is housed, shake up for subsequent use; With pencil on the TLC plate from horizontal line of bottom 1.5cm place picture, dip respectively a small amount of lactobionic acid solution and present embodiment product point sample on horizontal line with the capillary microcap, be labeled as respectively raw material point and product point (more than 2 point of sample horizontal interval 2cm).After methanol solvate is evaporated completely on the TLC plate, tiltedly puts into the high solvent of about 1cm (methyl alcohol: chromatography cylinder light petroleum ether=1: 15), and by sidewall, cover the chromatography cylinder loam cake is housed.After the race plate is finished, take out, dry up solvent on the TLC plate, put into the wide-mouth bottle for preparing in advance, close the lid, cover the TLC plate fully with silica gel.Take out after placing a period of time, can find out and all only have a large yellow point in the TLC plate above raw material point and product point, and 2 yellow points are not in the same horizontal line, this shows: the purity of raw material and product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.(same under the detection method)
Analyze after testing, in the product lactobionic acid chromium that present embodiment makes, chromium element mass fraction content is 4.5%, and the mass fraction content of lactobionic acid is 93.4%, and product lactobionic acid chromium molecular formula is Cr[C
12H
21O
12]
3, yield is that 95%(is in chromium), purity is 97.9%; Micro-constituent content analysis is Atomic absorption flame method mensuration in the product, the lactobionic acid content analysis is measured (list of references: MURAKAMI H with the original position fluorescent quantitation, KAWANO J, YOSHIZUMI H, et al.Screening of lactobionic acid producing microorganisms[J] .J Appl Glycosci, 2002,49 (4): 469-477).(same under the detection method)
Embodiment 2: the preparation of lactobionic acid copper complex.
In reactor, add 1.8t water, open and stir, again 250kg Salzburg vitriol and 745kg lactobionic acid are knocked down respectively (the lactobionic acid residue: the mol ratio of copper is about 2: 1) in the reactor, be warming up to 85 ℃, abundant stirring reaction 1.5h, the sodium hydroxide solution that constantly adds mass concentration 5% between the stage of reaction is about 7.0 with whole process control pH value; Cooling after reaction finishes slowly joins reaction system in the cyclohexane pond of constantly at the uniform velocity stirring again, and the adding speed of should controlling well is bad to prevent too fast caking or the crystal formation of causing of crystallization.Add and stop after complete stirring, leave standstill to the clarification of upper strata liquid, collect supernatant and deliver to the solvent recovery workshop and carry out cyclohexane and reclaim, it is reusable to reclaim the cyclohexane that obtains.Enter expansion drying after wet feed is centrifugal, obtain lactobionic acid copper 757kg.
Analyze after testing, copper mass fraction content in the present embodiment product lactobionic acid copper is 7.7%, the mass fraction content of lactobionic acid is 86.9%, and the mass fraction content of the crystallization water is: the 4.3%(product 120 ℃ of lower dehydrations to constant weight), product lactobionic acid copper molecule formula is Cu[C
12H
21O
12]
22H
2O, yield are that 93%(is in copper), purity is 98.9%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 3: the preparation of lactobionic acid ferrous coordination compound.
In reactor, add 1.8t water, open and stir, again 170kg Feromax and 760kg lactobionic acid are knocked down respectively (the lactobionic acid residue: ferrous mol ratio is about 2: 1) in the reactor, be warming up to 35 ℃, abundant stirring reaction 0.8h, the sodium hydroxide solution that constantly adds mass concentration 1% between the stage of reaction is about 5.5 with whole process control pH value; Cooling after reaction finishes slowly joins reaction system in the hexane pond of constantly at the uniform velocity stirring, and prevents that too fast caking or the crystal formation of causing of crystallization is bad.Stop after adding stirring, leave standstill to the clarification of upper strata liquid, the collection supernatant is delivered to the solvent recovery workshop and is carried out Recycling hexane, and it is reusable to reclaim the hexane that obtains.Enter expansion drying after wet feed is centrifugal, obtain the ferrous 734kg of lactobionic acid.
Analyze after testing, in the product lactobionic acid ferrous iron that present embodiment makes, ferrous element mass fraction content is 6.7%, and the mass fraction content of lactobionic acid is 85.9%, the mass fraction content of the crystallization water be the 4.3%(product 120 ℃ of lower dehydrations to constant weight), the ferrous molecular formula of product lactobionic acid is Fe[C
12H
21O
12]
22H
2O, yield are that 91%(is in ferrous iron), purity is 96.9%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 4: the preparation of lactobionic acid Zn complex.
In reactor, add 1.8t water, open and stir, knock down respectively 136kg zinc chloride and 730kg lactobionic acid in the reactor again, be warming up to 100 ℃ (the lactobionic acid residue: the mol ratio of zinc is about 2: 1), abundant stirring reaction 2.0h, the sodium hydroxide solution that constantly adds mass concentration 5% between the stage of reaction is about 7.0 with whole process control pH value; Cooling after reaction finishes slowly joins reaction system in the isopropyl ether pond of constantly at the uniform velocity stirring again, prevents that too fast caking or the crystal formation of causing of crystallization is bad.Stop after adding stirring, leave standstill to the clarification of upper strata liquid, the collection supernatant is delivered to the solvent recovery workshop and is carried out the isopropyl ether recovery, and it is reusable to reclaim the isopropyl ether that obtains.Enter expansion drying after wet feed is centrifugal, obtain lactobionic acid zinc 793kg.
Analyze after testing, the zinc element mass fraction content in the product lactobionic acid zinc is 7.8%, and the mass fraction content of lactobionic acid is 85.6%, the mass fraction content of the crystallization water be the 4.3%(product 120 ℃ of lower dehydrations to constant weight), product lactobionic acid zinc molecular formula is Zn[C
12H
21O
12]
22H
2O, yield are that 97%(is in zinc), purity is 97.7%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 5: the preparation of lactobionic acid manganese complex.
In reactor, add 1.5t water, open and stir, again 198kg four hydration manganese chlorides and 730kg lactobionic acid are knocked down respectively (the lactobionic acid residue: the mol ratio of manganese is about 2: 1) in the reactor, be warming up to 100 ℃, abundant stirring reaction 2.0h, the omnidistance control of the sodium hydroxide solution pH value that constantly adds mass concentration 5% between the stage of reaction is about 7.0; Cooling after reaction finishes slowly joins reaction system in the isopropyl ether pond of constantly at the uniform velocity stirring again, prevents that too fast caking or the crystal formation of causing of crystallization is bad.Stop after adding stirring, leave standstill to the clarification of upper strata liquid, the collection supernatant is delivered to the solvent recovery workshop and is carried out the isopropyl ether recovery, and it is reusable to reclaim the isopropyl ether that obtains.Enter expansion drying after wet feed is centrifugal, obtain lactobionic acid manganese 773kg.
Analyze after testing, manganese element mass fraction content in the product lactobionic acid manganese is 6.7%, the mass fraction content of lactobionic acid is 86.9%, and the mass fraction content of the crystallization water is: the 4.4%(product 120 ℃ of lower dehydrations to constant weight), product lactobionic acid manganese molecular formula is Mn[C
12H
21O
12]
22H
2O, yield are that 96%(is in manganese), purity is 98.0%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 6: the preparation of lactobionic acid cobalt complex.
28.1g Cobalt monosulfate heptahydrate and 28.6g lactobionic acid are added (the lactobionic acid residue: the mol ratio of cobalt is about 2: 1) in the 60ml water together, be warming up to 85 ℃, abundant stirring reaction 1.0h, the omnidistance control of potassium hydroxide solution pH value with mass concentration 5% is about 4.5, cooling slowly joined crystallization in the benzinum with reaction system again after reaction finished.Filter after leaving standstill 15min, thing is put into 60 ℃ of dry 2h of baking oven in the filter, obtains 76.1g lactobionic acid cobalt.Benzinum in the filtrate is reclaimed, and it is reusable to reclaim the benzinum that obtains.
Analyze after testing, in the product lactobionic acid cobalt that present embodiment makes, cobalt element mass fraction content is 6.7%, and the mass fraction content of lactobionic acid is 82.8%, the mass fraction content of the crystallization water be the 8.3%(product 120 ℃ of lower dehydrations to constant weight), product lactobionic acid cobalt molecular formula is Co[C
12H
21O
12]
24H
2O, yield are that 90%(is in cobalt), purity is 97.8%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 7: the preparation of lactobionic acid nickel complex.
26.3g six hydration nickel sulfate and 36.0g lactobionic acid are added (the lactobionic acid residue: the mol ratio of nickel is about 2: 1) in the 60ml water together, be warming up to 85 ℃, abundant stirring reaction 1.0h, the omnidistance control of sodium hydroxide solution pH value with mass concentration 5% is about 4.0, cooling slowly joined crystallization in the isopropyl ether with reaction system again after reaction finished.Filter after leaving standstill 15min, thing is put into 60 ℃ of dry 2h of baking oven in the filter, obtains 68.8g lactobionic acid nickel.Isopropyl ether in the filtrate is reclaimed, and it is reusable to reclaim the isopropyl ether that obtains.
Analyze after testing, nickel element mass fraction content in the product lactobionic acid nickel that present embodiment makes is 7.1%, the mass fraction content of lactobionic acid is 86.5%, the mass fraction content of the crystallization water be the 4.3%(product 120 ℃ of lower dehydrations to constant weight), product lactobionic acid nickel molecular formula is Ni[C
12H
21O
12]
22H
2O, yield are that 85%(is in nickel), purity is 97.9%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 8: the preparation of lactobionic acid Complex of Ge.
7.3g germanous chloride and 29.0g lactobionic acid are added (the lactobionic acid residue: the mol ratio of germanium is about 2: 1) in the 50ml water together, be warming up to 85 ℃, abundant stirring reaction 0.5h, the omnidistance control of potassium hydroxide solution pH value with mass concentration 5% is about 9.0, cooling slowly joined crystallization in the benzinum with reaction system again after reaction finished.Filter after leaving standstill 15min, thing is put into 60 ℃ of dry 2h of baking oven in the filter, obtains 64.5g lactobionic acid germanium.Benzinum in the filtrate is reclaimed, and it is reusable to reclaim the benzinum that obtains.
Analyze after testing, the Ge element mass fraction content in the present embodiment product lactobionic acid germanium is 9.0%, and the mass fraction content of lactobionic acid is 89.0%, and product lactobionic acid germanium molecular formula is Ge[C
12H
21O
12]
2, yield is that 82%(is in germanium), purity is 98.0%.The product purity qualitative analysis: the purity of product is very high, the range estimation free from admixture, and the product that reacts after finishing is not raw material.
Embodiment 9: the application that conduct improves the feed addictive of duckling immunity in the duck feed of lactobionic acid manganese.
The feeding experiment that the lactobionic acid manganese of preparation among the embodiment 5 is used for duckling.Select 1 age in days without 80 of the Beijing ducklings of duck virus hepatitis vaccine inoculation, be divided at random 4 groups, 20 every group, raise on the Fen Quan plane, every group one circle.24h constant light photograph, free choice feeding and drinking-water.Experimental period 30d, off-test is slaughtered duckling take group as unit, collects blood, thymus gland, spleen and analysis.Wherein, control group is duckling manganese deficiency basal diet; Test one group and add lactobionic acid manganese in duckling manganese deficiency daily ration, lactobionic acid manganese addition is counted 50ppm with manganese element in the daily ration per ton; Test two groups and in duckling manganese deficiency daily ration, add lactobionic acid manganese, lactobionic acid manganese addition is counted 150ppm with manganese element in the daily ration per ton, test three groups and add manganese sulfate monohydrate in duckling manganese deficiency daily ration, the manganese sulfate monohydrate addition is counted 150ppm with manganese element in the daily ration per ton.Result of the test sees Table 1.
Table 1: lactobionic acid manganese is on the impact of duckling immune parameter
As can be seen from Table 1, the clear to some extent difference of spending all of thymus gland, spleen and serum HI between control group and test group.Thymus gland and spleen weight average that test group is compared control group increase to some extent, and serum HI spends clearly decline.Test one group and test between two groups, along with the increase of manganese addition, the weight of thymus gland and spleen is in rising trend, and the clear degree of serum HI is on a declining curve; And be all and add 150ppm manganese, the data of testing two groups are better than three groups of tests.This shows, add manganese and can strengthen the immunity of duckling.Lactobionic acid manganese will be significantly better than manganese sulfate monohydrate to the effect that improves duckling immunity; And along with the raising of lactobionic acid manganese addition, the effect that improves duckling immunity is more obvious.
Embodiment 10: lactobionic acid nickel in the chicken feed as promoting growth of meat chicken, improving the application of the feed addictive of broiler carcass quality.
The feeding experiment that the lactobionic acid nickel of preparation among the embodiment 7 is used for broiler chicken.Select 60 1 age in days meat chicks from the hatching of same hatchery (Ross * Ross).Chicken places ground cage (1.5m * 3m), make bedding and padding with sawdust, young bird daily ration to 20 age in days of feeding.After when 21 age in days, weighing, with 60 cock mothers half and half, be divided at random 1 control group and 2 processed group, every group of 2 repetitions, each repeats 10 chickens.Experimental period was 4 weeks.Wherein, control group is fed broiler chicken and is lacked the nickel daily ration, process 1 group of lactobionic acid nickel that in the scarce nickel daily ration of broiler chicken, adds present embodiment, lactobionic acid nickel addition is counted 3ppm with nickel element in the daily ration per ton, process 2 groups and add lactobionic acid nickel in the scarce nickel daily ration of broiler chicken, lactobionic acid nickel addition is counted 10ppm with nickel element in the daily ration per ton.Record weekly 1 feed intake and BW, butcher broiler chicken when 48 age in days, remove feather, ketoboidies is being weighed after extracing internal organ and abdomen fat piece behind-20 ℃ of lower freezing diels.Trunk after the processing is pulverized post analysis water, protein, fat and ash content twice by 31.75ram mill mangler, and all data are averaged as shown in table 2 below.
Table 2: lactobionic acid nickel is on the impact of growth of meat chicken performance and carcass composition
As can be seen from Table 2: growth performance differs greatly between processed group and control group; Carcass composition is variant.Although each processed group is little with control group difference on the trunk moisture content index, but differ greatly in weightening finish, feed intake, feedstuff-meat ratio, abdomen fat, protein, fat, ash content index, raising along with lactobionic acid nickel addition, its feed intake and weightening finish are improved trend, feedstuff-meat ratio has downward trend, fat content in abdomen fat content and the carcass composition has downward trend, and the protein content in the carcass composition and ash content (mineral matter element) content is on the rise.This shows that the lactobionic acid nickel of the present invention's preparation has good growth-promoting effect to broiler chicken, has improved the quality (improved protein and mineral matter element, reduced fat content) of trunk.
Embodiment 11: the application of the feed addictive that lactobionic acid germanium is grown as the promotion weanling pig in pig feed.
The feeding experiment that the lactobionic acid germanium of preparation among the embodiment 8 is used for weanling pig.Select 108 of the healthy anosis weanling pigs that kind consistent (Du * long * large), birth age in days approach, be divided at random control group and two test group, every group of 6 repetitions, each repeats 6 pigs, as feed addictive, control group is that pig is with lacking the germanium daily ration to test group with lactobionic acid germanium.Wherein, test one group of pig per ton and count 3ppm with lactobionic acid germanium addition in the scarce germanium daily ration with Ge element, test two groups of pigs per ton and count 10ppm with lactobionic acid germanium addition in the scarce germanium daily ration with Ge element, feeding time was 2 weeks, and effect is as shown in table 3 below:
Table 3: lactobionic acid germanium is on the impact of Growth Performance of Weaning Piglets
The data of above-mentioned table 3 show: speed of growth significant difference between test group and control group; Although each test group is not remarkable with control group difference on the feed intake index, significant difference on weightening finish and feedstuff-meat ratio index, along with the raising of lactobionic acid germanium addition, its weightening finish is improved trend, and feedstuff-meat ratio has downward trend.This shows that lactobionic acid germanium has good growth-promoting effect to weanling pig.
Embodiment 12: the conduct in pig feed of lactobionic acid ferrous iron promotes the application of the feed addictive of growth pig hematopoiesis.
With the ferrous feeding experiment that is used for the growth pig of the lactobionic acid of preparation among the embodiment 3.Select 144 of healthy anosis growth pigs about kind consistent (Du * long * large), 28 ages in days, be divided at random control group and three test group, every group of 6 repetitions, each repeats 6 pigs, as feed addictive, control group only feeds pigs and uses the iron deficiency daily ration test group with the lactobionic acid ferrous iron.Wherein, test one group at pig interpolation lactobionic acid ferrous iron in the iron deficiency daily ration, addition in the feed per ton is counted 100ppm with ferrous element, test two groups at pig interpolation lactobionic acid ferrous iron in the iron deficiency daily ration, addition in the feed per ton is counted 200ppm with ferrous element, tests three groups at pig interpolation lactobionic acid ferrous iron in the iron deficiency daily ration, and the addition in the feed per ton is counted 750ppm with ferrous element, feeding time was 3 weeks, body weight before and after record feed intake and the experiment.And take vena cava anterior to get blood in feed the 7th day, the 14th day and the 21st day to the growth pig respectively, and after anti-freezing is processed, Measuring hemoglobin content.Experimental result is as shown in table 4 below.
Table 4: the lactobionic acid ferrous iron is on the impact of growth PINPROL content (g/100ml)
The data of above-mentioned table 4 show: the content difference of hemoglobin is remarkable between test group and control group; And significant difference between test group, and along with the raising of the ferrous addition of lactobionic acid, its content of hemoglobin is improved trend.This shows can well promote the to grow hematopoiesis of pig of lactobionic acid ferrous iron, the blows that prevents from growing (the regulation HC 8g/100ml of Shanghai animal doctor's herding association in 1979 is critical anaemia point).
Embodiment 13: the application of the feed addictive that lactobionic acid chromium is fattened as the promotion fattening pig in pig feed.
The feeding experiment that the lactobionic acid chromium of preparation among the embodiment 1 is used for fattening pig.Select 90 fattening pigs that age in days is close, weight differential is little (ternary hybrid pig) as test pig, according to body weight, principle that sex ratio is identical, be divided at random two groups of control group and one group of test, tests, every group of 3 repetitions, each repeats 10 fattening pigs, and the nursing time is 35 days.Wherein control group is fed and is lacked the chromium daily ration, tests and adds lactobionic acid chromium in one group of scarce chromium daily ration per ton, and addition is counted 0.1ppm with the chromium element; Test and add lactobionic acid chromium in two groups of scarce chromium daily rations per ton, addition is counted 0.2ppm with the chromium element.Result of the test sees Table 5.
Table 5: lactobionic acid chromium is on the impact of Growth of Pigs performance
As can be seen from Table 5, average daily gain, the feedstuff-meat ratio index at duration of test test group and control group reaches significant difference; Significant difference between test group.The result of above-mentioned table 5 shows: the speed of growth significant difference of fattening pig between test group and control group; Significant difference on feed intake, weightening finish and the feedstuff-meat ratio index, along with the raising of lactobionic acid chromium addition, its weightening finish is improved trend, feedstuff-meat ratio has downward trend, and showing lactobionic acid chromium has good fattening effect to fattening pig.
Embodiment 14: the application that conduct improves the feed addictive of brood sow reproductive performance in pig feed of lactobionic acid zinc.
The feeding experiment that the lactobionic acid zinc of preparation among the embodiment 4 is used for brood sow.Select 60 of the Du Luoke sows of same parity, be divided into three groups (one, two group of control group and test), every group 20, breed with 2～3 Duroc boars for every group, requirement is finished breeding within an oestrus, wherein control group is tested and is added lactobionic acid zinc in one group of scarce zinc daily ration per ton for lacking the zinc daily ration, and addition is counted 100ppm with the zinc element; Test and add lactobionic acid zinc in two groups of scarce zinc daily rations per ton, addition is counted 200ppm with the zinc element.Statistics is nascent, the young number of work and the litter weight of 20 ages in days, 40 ages in days, and result of the test sees Table 6.
Table 6: lactobionic acid zinc is on the impact of brood sow production performance
As can be seen from Table 6, for the nascent young number of work, control group and test group reach significant difference, but on the not impact of nascent litter weight; Along with the variation of feeding time, the young number of the work of its 20 age in days also changes thereupon, and litter weight begins to show larger difference, and survival rate has the difference of conspicuousness; During 40 age in days, the performance of the young number of living is similar to 20 ages in days, and incubation rate also reaches significant difference.Above application result shows: add the test group of lactobionic acid zinc, the young number of the work of brood sow, the survival rate in later stage, incubation rate all have the raising of conspicuousness than control group, and litter weight also is greatly improved than control group.This shows lactobionic acid zinc the reproductive performance of brood sow is improved a lot.
Embodiment 15: the application of the feed addictive that the lactobionic acid cobalt is grown as the promotion calf in ruminant feed.
The feeding experiment that the lactobionic acid cobalt of preparation among the embodiment 6 is used for calf.40 calves (80～90 age in days) of selecting the hybridization of same black-and-white flower breeding oxen and primiparity Qin Chuan cow to give birth to are the test ox, are divided at random 4 groups, 10 every group.Wherein control group is tested and is added the lactobionic acid cobalt in one group of scarce cobalt daily ration per ton for lacking the cobalt daily ration, and addition is counted 0.2ppm with cobalt element; Test and add the lactobionic acid cobalt in two groups of scarce cobalt daily rations per ton, addition is counted 2ppm with cobalt element.Experimental period, is 37d altogether, and wherein preliminary trial period 7d is just trying phase 30d.Result of the test sees Table 7.
Table 7: the lactobionic acid cobalt is on the impact of calf growth performance
As can be seen from Table 7, under the condition that initial body weight is more or less the same, in the average daily gain of duration of test test group and control group significant difference is arranged, and significant difference between test group.The result of above-mentioned table 7 shows: the speed of growth significant difference of calf between test group and control group; And along with the raising of lactobionic acid cobalt addition, its daily gain is improved trend, and showing the lactobionic acid cobalt has good growth-promoting effect to calf.
Embodiment 16: the application of lactobionic acid copper in Russian carp
The feeding experiment that the lactobionic acid copper of preparation among the embodiment 2 is used for Russian carp.Selecting healthy, even, initial body weight is Russian carp 900 tails of (140.4 ± 2.0) g, is equally divided into 3 groups, and each processes 3 repetitions, and each repeats 100 tails, and each pond is 1 and repeats cement pit specification 6m * 9m.Fingerling is bought the same day back and is divided into groups immediately, after the grouping with the anti-bacteria and virus sterilization of splashing; Dead fish is selected the fish substitute for subsequent use that specification approaches in the 1st week, progressively finishes testing material to the replacement of former feed in the 3d after the grouping; Duration of test is thrown something and fed 4 times under normal circumstances every day, and starts aerator according to weather condition in good time; The every two weeks of duration of test changes weekly water 1/3 with chlorinated product sterilization 1 time, and dissolved oxygen keeps more than the 6mg/L, experimental period 70d.Wherein control group is tested and is added lactobionic acid copper in one group of scarce copper daily ration per ton for lacking the copper daily ration, and addition is counted 2ppm with copper; Test and add lactobionic acid copper in two groups of scarce copper daily rations per ton, addition is counted 25ppm with copper.Crude protein 35.00% in its scarce copper daily ration, crude fat 5.40%, available phosphorus 0.60%.Result of the test sees the following form 8.
Table 8: lactobionic acid copper is on the growth performance impact of Russian carp
As can be seen from Table 8: test group and control group do not have significant change taking the photograph the bait amount, but on weightening finish, the relative weight gain rate, feed coefficient and the death rate significant difference are arranged all, and significant difference between test group.Above-mentioned table 8 result shows: the growth of Russian carp and survival difference on effect are remarkable between test group and control group; And along with the raising of lactobionic acid copper addition, the relative weight gain rate is improved trend, and feed coefficient and the death rate all have a declining tendency, and showing lactobionic acid copper has good growth result to Russian carp.
Claims (10)
1. the preparation method of lactobionic acid trace element complex, may further comprise the steps: inorganic microelement compound and lactobionic acid are added to the water together, abundant stirring reaction after heating up, add acid solution or add the omnidistance pH value of controlling reaction system of alkaline solution, cooling after reaction finishes, reaction system is slowly joined crystallization in the organic solvent, then carry out Separation of Solid and Liquid, the solid matter after the separation namely obtains lactobionic acid trace element complex after super-dry.
2. the preparation method of lactobionic acid according to claim 1 trace element complex, it is characterized in that: described inorganic microelement compound comprises copper compound, ferro element compound, zinc element compound, manganese element compound, chromium element compound, cobalt element compound, nickel element compound or Ge element compound;
Described copper compound comprises at least a in copper chloride, copper sulphate, cupric oxide, Kocide SD, basic copper chloride, basic copper sulfate, the basic copper carbonate;
Described ferro element compound comprises at least a in frerrous chloride, ferrous sulfate, the ferrous carbonate;
Described zinc element compound comprises at least a in zinc chloride, zinc sulfate, zinc hydroxide, zinc oxide, basic zinc sulfate, basic zinc carbonate, the basic zinc chloride;
Described manganese element compound comprises at least a in manganous chloride, manganese sulfate, manganous hydroxide, manganous oxide, the alkali formula manganese sulfate;
Described chromium element compound comprises at least a in chromium trichloride, chromium sulfate, chromium hydroxide, the zinc tetroxychromate;
Described cobalt element compound comprises at least a in cobalt oxide, cobalt hydroxide, cobalt chloride, cobaltous sulfate, cobalt carbonate, the basic cobaltous carbonate;
Described nickel element compound comprises at least a in nickel oxide, nickel hydroxide, nickel chloride, nickelous sulfate, nickelous carbonate, the basic nickel carbonate;
Described Ge element compound comprises at least a in germanium oxide, germanous chloride, calcium germinate, germanic acid copper, sodium metagermanate, two sodium germanates, four sodium germanates.
3. the preparation method of lactobionic acid according to claim 2 trace element complex, it is characterized in that: the molar ratio of metallic element and described lactobionic acid is 1: 0.8～3.5 in the described inorganic microelement compound.
4. according to claim 1, the preparation method of 2 or 3 described lactobionic acids trace element complexs, it is characterized in that: the pH value whole process of described reaction system is controlled at 4～9.
5. according to claim 1, the preparation method of 2 or 3 described lactobionic acids trace element complexs, it is characterized in that: the reaction temperature in the described stirring reaction process is controlled at 35 ℃～100 ℃, and the reaction time is 0.5h～3h.
6. according to claim 1, the preparation method of 2 or 3 described lactobionic acids trace element complexs, it is characterized in that: described organic solvent is at least a in isopropyl ether, isopentane, pentane, benzinum, hexane, thiacyclohexane, the pentamethylene.
7. one kind such as the application as feed addictive in pig, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal of the preparation method makes as described in each in the claim 1～6 lactobionic acid trace element complex.
8. application according to claim 7 is characterized in that, described lactobionic acid trace element complex is lactobionic acid copper, lactobionic acid ferrous iron, lactobionic acid zinc, lactobionic acid manganese, lactobionic acid chromium, lactobionic acid cobalt, lactobionic acid nickel or lactobionic acid germanium.
9. application according to claim 8 is characterized in that:
The addition of described lactobionic acid copper in pig feed per ton counted 2ppm～200ppm with copper; The addition of described lactobionic acid copper in the feed of duck per ton, broiler chicken, ruminant or goose counted 0.1ppm～35ppm with copper; Described lactobionic acid copper addition in aquatic animal feed per ton is counted 0.1ppm～25ppm with copper; Described lactobionic acid copper addition in pet per ton or special economic animal feed is counted 0.1ppm～200ppm with copper;
The addition of described lactobionic acid ferrous iron in pig per ton, duck, broiler chicken, goose, ruminant or aquatic livestock counted 20ppm～750ppm with ferrous element; The addition of described lactobionic acid ferrous iron in feed for pet per ton or special economic animal feed counted 10ppm～1250ppm with ferrous element;
The addition of described lactobionic acid zinc in pig feed per ton counted 10ppm～200ppm with the zinc element; The addition of described lactobionic acid zinc in duck per ton, broiler chicken, goose or ruminant feed counted 10ppm～150ppm with the zinc element; The addition of described lactobionic acid zinc in aquatic animal feed per ton counted 5ppm～200ppm with the zinc element; The addition of described lactobionic acid zinc in feed for pet per ton or special economic animal feed counted 5ppm～250ppm with the zinc element;
The addition of described lactobionic acid manganese in pig per ton, duck, broiler chicken, goose, ruminant, pet or special economic animal feed counted 5ppm～150ppm with manganese element; Described lactobionic acid manganese addition in aquatic animal feed per ton is counted 5ppm～100ppm with manganese element;
The addition of described lactobionic acid chromium in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～0.2ppm with the chromium element;
The addition of described lactobionic acid cobalt in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～2ppm with cobalt element;
The addition of described lactobionic acid nickel in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～10ppm with nickel element;
The addition of described lactobionic acid germanium in pig per ton, duck, broiler chicken, goose, aquatic livestock, ruminant, pet or special economic animal feed counted 0.01ppm～10ppm with Ge element.
10. application according to claim 9 is characterized in that:
The application of described lactobionic acid copper specifically refers to the application as the feed addictive that promotes Russian carp growth in aquatic animal feed of lactobionic acid copper, and the addition of described lactobionic acid copper in Russian carp feed per ton counted 2ppm～25ppm with copper;
The application of described lactobionic acid ferrous iron specifically refers to the application as the feed addictive that promotes the hematopoiesis of growth pig in pig feed of lactobionic acid ferrous iron, and the addition of described lactobionic acid ferrous iron in pig feed per ton counted 100ppm～750ppm with ferrous element;
The application of described lactobionic acid zinc specifically refers to the application as the feed addictive that improves the brood sow reproductive performance in pig feed of lactobionic acid zinc, and the addition of described lactobionic acid zinc in brood sow feed per ton counted 100ppm～200ppm with the zinc element;
The application of described lactobionic acid manganese specifically refers to the application as the feed addictive that improves duckling immunity in the duck feed of lactobionic acid manganese, and the addition of described lactobionic acid manganese in duckling feed per ton counted 50ppm～150ppm with manganese element;
The application of described lactobionic acid chromium specifically refers to the application as the feed addictive that promotes fattening pig fattening in pig feed of lactobionic acid chromium, and the addition of described lactobionic acid chromium in pig feed per ton counted 0.1ppm～0.2ppm with inferior chromium element;
The application of described lactobionic acid cobalt specifically refers to the application as the feed addictive that promotes the calf growth in ruminant feed of lactobionic acid cobalt, and the addition of described lactobionic acid cobalt in calf feed per ton counted 0.2ppm～2ppm with cobalt element;
The application of described lactobionic acid nickel specifically refer to lactobionic acid nickel in the chicken feed as promoting growth of meat chicken, improve the application of the feed addictive of broiler carcass quality that the addition of described lactobionic acid nickel in broiler fodder per ton counted 3ppm～10ppm with nickel element;
The application of described lactobionic acid germanium specifically refers to the application as the feed addictive that promotes the weanling pig growth in pig feed of lactobionic acid germanium, and described lactobionic acid germanium addition in weanling pig feed per ton is counted 3ppm～10ppm with Ge element.
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| CN105600817A (en) * | 2016-01-22 | 2016-05-25 | 广州先至饲料添加剂有限公司 | Zinc sulfate and calcium composition and application thereof in preparing feed additive |
| CN107032410A (en) * | 2017-04-24 | 2017-08-11 | 长沙兴嘉生物工程股份有限公司 | The preparation method and applications of alkali formula manganese sulfate |
| CN111248353A (en) * | 2020-03-17 | 2020-06-09 | 南宁市泽威尔饲料有限责任公司 | Micro-emulsion type liquid organic trace element feed additive and preparation method thereof |
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| CN1526317A (en) * | 2003-02-28 | 2004-09-08 | ����ʳƷ���Ź�˾ | Lactin acid mineral compound and its application at food field of mineral subtance nutrition intensification |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105600817A (en) * | 2016-01-22 | 2016-05-25 | 广州先至饲料添加剂有限公司 | Zinc sulfate and calcium composition and application thereof in preparing feed additive |
| CN107032410A (en) * | 2017-04-24 | 2017-08-11 | 长沙兴嘉生物工程股份有限公司 | The preparation method and applications of alkali formula manganese sulfate |
| CN107032410B (en) * | 2017-04-24 | 2019-04-05 | 长沙兴嘉生物工程股份有限公司 | The preparation method and applications of alkali formula manganese sulfate |
| CN111248353A (en) * | 2020-03-17 | 2020-06-09 | 南宁市泽威尔饲料有限责任公司 | Micro-emulsion type liquid organic trace element feed additive and preparation method thereof |
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