CN109619287B - Application of purine - Google Patents

Application of purine Download PDF

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CN109619287B
CN109619287B CN201910100327.9A CN201910100327A CN109619287B CN 109619287 B CN109619287 B CN 109619287B CN 201910100327 A CN201910100327 A CN 201910100327A CN 109619287 B CN109619287 B CN 109619287B
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束刚
游慧
尹聪
王丽娜
王松波
高萍
朱晓彤
江青艳
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South China Agricultural University
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
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    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A61K31/52—Purines, e.g. adenine
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    • A61K31/52—Purines, e.g. adenine
    • A61K31/522—Purines, e.g. adenine having oxo groups directly attached to the heterocyclic ring, e.g. hypoxanthine, guanine, acyclovir
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract

The invention relates to the technical field of biotechnology, and provides application of purine, which comprises the following steps: by allowing the organism to ingest the purine to promote growth of the organism or promote the organism to increase muscle content or improve metabolism of the organism and maintain body temperature or promote feeding of the organism or promote organ development of the organism or promote the organism to lower blood sugar, the organism includes livestock, aquatic organisms and human beings.

Description

Application of purine
Technical Field
The invention relates to the technical field of biotechnology, in particular to application of purine.
Background
Purine is a substance existing in the body, mainly exists in the form of purine nucleotide, plays an important role in energy supply, metabolic regulation, composition of coenzyme and the like, but generally, only the form of purine nucleotide is concerned, and actually exists in the form of purine in the organism.
In the prior art, purine nucleotides are studied more, but purine is still studied less intensively.
A Chinese patent with publication No. CN1589896A, its preparing process and its application in preparing the medicines for treating SARS and its complication disclose that hypoxanthine is added to said medicine composition to promote the metabolism of heart and brain tissue, participate in the growth, division and regeneration of brain tissue neurons, improve cerebral blood circulation and cerebral metabolism function, and can be used to treat the dysfunction caused by myocardial and cerebral diseases.
In the prior art, the research on purine is limited to the application of cardiovascular and cerebrovascular diseases, is very weak, and still needs further research to develop the application of purine.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the application of purine, which has the advantages of promoting the growth of organisms, increasing the muscle content, improving the metabolism, maintaining the body temperature, promoting the food intake, promoting the development of organs and reducing the blood sugar.
In order to achieve the purpose, the invention provides the following technical scheme:
use of a purine for promoting the growth of an organism or for promoting an organism to increase muscle content or for increasing the metabolism of an organism and maintaining body temperature or for promoting an organism to feed or for promoting the development of an organism organ or for promoting an organism to lower blood glucose by having an organism ingest said purine.
The invention is further configured to: the organisms include livestock, including, for example, chickens, ducks, geese, pigs, cattle, sheep, horses, dogs, cats, rats, etc.
The invention is further configured to: the organisms include aquatic organisms including, for example, fish, shrimp, crab, and the like.
The invention is further configured to: the purine includes one or more of hypoxanthine, xanthine, adenine and guanine.
The invention is further configured to: the purine is used for preparing feed.
The invention is further configured to: the mass content of purine in the feed is 0.01% -2%.
In conclusion, the invention has the following beneficial effects:
in the present invention, the mode of biological purine intake includes injection, abdominal irrigation, solid or liquid feeding, etc.
In the invention, the experimental organisms 'mouse, hen and piglet' are taken as examples to feed containing purine and feed containing purine nucleotide respectively, the feed containing purine is taken as an experimental group, the feed containing purine nucleotide is taken as a control group, and the weight increment, muscle increment, fat increment, muscle rate, pectoral muscle rate, 24h oxygen consumption, 24h respiratory entropy, body temperature reduction value in 4 ℃ environment and the change of purine content in serum of the experimental group and the control group are compared to verify the application effect of purine.
In the present invention, purine may be added in feed, drinking water, injection, oral liquid, medicine, etc.
In the present invention, the content of purine in feed, drinking water, injection, oral liquid, drug, and other substances may be 0.01%, 0.03%, 0.05%, 0.08%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.3%, 1.5%, 1.8%, 2%, and the like.
The purine can effectively improve the content of biological muscle, promote the growth of organisms, improve the metabolic rate of the organisms, reduce the blood sugar of the organisms, keep the body temperature of the organisms more constant and promote the development of biological organs.
Detailed Description
The present invention will be described in further detail with reference to examples.
In the following examples, the feed supplemented with purine or purine nucleotide was obtained by mixing purine or purine nucleotide with a common feed, which was corn grain.
Example 1
In this example, C57BL/6J mice (purchased from Guangdong provincial animal center for medical experiments) were used.
After being fed for one week, 16 female mice with five weeks of age are divided into two groups according to the weight, wherein each group is divided into 8 mice, the two groups are divided into an experimental combination control group, the control group is fed with a feed containing hypoxanthine nucleotide and the weight content of the feed is 0.01%, and the experimental group is fed with a feed containing hypoxanthine and the weight content of the feed is 0.01%.
The breeding period is 6 weeks, and the breeding is carried out at 25 deg.C in 12h/12h light/dark cycle environment, and the people can eat and drink water freely.
And recording the mean weight gain, the mean daily feed intake, the mean muscle weight gain, the mean fat weight gain, the mean 24h oxygen consumption and the mean 24h respiratory entropy of the mice of the experimental group and the control group after experimental feeding for 6 weeks.
And (3) placing the experimental group and the control group which are raised for 6 weeks in the experiment into an environment of 4 ℃ for cold stimulation, and recording the average value of the body temperature reduction values of each experimental group and the control group in the environment of 4 ℃.
After the cold stimulation experiment is completed, the experimental group and the control group are sacrificed, and the weight mean value of the liver and the weight mean value of the spleen of the mice in the experimental group and the control group are recorded; and measuring the mean value of the hypoxanthine content in the serum of the mice of the experimental group and the control group.
Example 2
The difference from example 1 is that:
the experimental group was fed with a feed containing hypoxanthine in an amount of 0.08% by weight.
The control group was fed with a feed containing inosinic acid in an amount of 0.08% by weight.
Example 3
The difference from example 1 is that:
the experimental group was fed with a feed containing hypoxanthine in an amount of 1.4% by weight.
The control group was fed with a feed containing inosinic acid in an amount of 1.4% by weight.
Example 4
The difference from example 1 is that:
the experimental group was fed with 2% by weight feed containing hypoxanthine.
The control group was fed with 2% by weight of a feed containing inosinic acid.
Example 5
The difference from example 1 is that:
the experimental group was fed with a feed containing xanthine and having a weight content of 0.01%.
The control group was fed with a feed containing 0.01% by weight of xanthine nucleotide. And measuring the mean value of the hypoxanthine content in the serum of the mice in the experimental group and the control group.
Example 6
The differences from example 5 are:
the experimental group was fed with a feed containing xanthine and having a weight content of 0.08%.
The control group was fed with a feed containing 0.08% by weight of xanthine nucleotide.
Example 7
The difference from example 5 is that:
the experimental group was fed with a feed containing 1.4% by weight of xanthine.
The control group was fed with a feed containing 1.4% by weight of xanthine nucleotide.
Example 8
The differences from example 5 are:
the experimental groups were fed with 2% by weight feed containing xanthine.
The control group was fed with 2% by weight of feed containing xanthine nucleotide.
Example 9
The difference from example 1 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.01% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.01% by weight. And measuring the mean value of the hypoxanthine content in the serum of the mice in the experimental group and the control group.
Example 10
The difference from example 9 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.08% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.08% by weight.
Example 13
The difference from example 1 is that:
the experimental group was fed with a feed containing guanine in an amount of 0.01% by weight.
The control group was fed with a feed containing guanine nucleotide at a weight content of 0.01%. And measuring the mean value of the hypoxanthine content in the serum of the mice in the experimental group and the control group.
Example 14
The difference from example 13 is that:
the experimental group was fed with a feed containing guanine in an amount of 0.08% by weight.
The control group was fed with a feed containing guanine nucleotide in an amount of 0.08% by weight.
Example 15
The difference from example 13 is that:
the experimental group was fed with 1.4% by weight of feed containing guanine.
The control group was fed with 1.4% by weight of feed containing guanine nucleotide.
Example 16
The difference from example 13 is that:
the experimental groups were fed with 2% by weight of feed containing guanine.
The control group was fed with a feed containing 2% by weight of guanine nucleotide.
Specific experimental data for examples 1-16 are shown in tables 1-4.
Table 1 shows experimental data for examples 1-4.
TABLE 1
Figure GDA0003907919680000061
Table 2 shows experimental data for examples 5 to 8.
TABLE 2
Figure GDA0003907919680000062
Figure GDA0003907919680000071
Table 3 shows the experimental data for examples 9 to 12.
TABLE 3
Figure GDA0003907919680000072
Table 4 shows experimental data for examples 13 to 16.
TABLE 4
Figure GDA0003907919680000081
As can be seen from tables 1 to 4, when the mice were fed with the feed supplemented with the purines such as hypoxanthine, xanthine, guanine and adenine, the average daily food intake and the average weight gain of the mice were significantly improved as compared with the case where the mice were fed with the purine nucleotides such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, which proved that the addition of the purine to the feed significantly promoted the feeding of the mice and further increased the weight of the mice to promote the growth of the mice more rapidly;
when the mice are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the muscle weight mean value of the mice is higher and the fat weight mean value of the mice is lower than that when the mice are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, the muscle increment of the mice is proved to be increased and the fat increment is proved to be reduced because the initial physiological states of a plurality of mice are close to each other, and the feed added with the purine is proved to be obviously beneficial to increasing the muscle content of the mice and enabling the mice to be stronger;
when the mice are fed with purine feeds such as hypoxanthine, xanthine, guanine and adenine, the feed consumption of the mice for 24 hours is higher than that of the mice fed with purine nucleotides such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the metabolic rate of the mice is proved to be accelerated;
when the mice are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the average value of the 24-hour respiratory entropy of the mice is closer to 1 than when the mice are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, so that the mice are proved to decompose a large amount of sugar when consuming energy, and the blood sugar of the mice is favorably reduced;
when the mice are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the mean value of the 4 ℃ body temperature drop values of the mice is reduced compared with the feed added with the purine nucleotides such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, so that the mice are proved to have better capability of adjusting the body temperature and better respond to cold stimulation;
when the mice are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the liver weight average value and the spleen weight average value of the mice are higher than those of the mice fed with the feed added with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the visceral organs of the mice grow faster and better as the initial physiological states of a plurality of mice are close to each other;
when a feed containing a purine such as hypoxanthine, xanthine, guanine and adenine is fed to a mouse, the mean purine concentration in the serum of the mouse is higher than that when a feed containing a purine nucleotide such as inosinic acid, xanthosine, guanine nucleotide and adenine nucleotide is fed to a mouse, and it is proved that the main reason for the effect is that the concentration of purine in the mouse is adjusted by the ingestion of purine from the feed, and the effects of promoting growth, increasing muscle content, lowering blood sugar, promoting metabolism, maintaining body temperature more stably, promoting organ growth, and the like are exerted.
In the prior art, mice are often used as substitutes for human experiments, so that the effects of promoting metabolism, reducing blood sugar and the like can be deduced through the experiments of the mice after the human beings take purine.
Example 17
In this example, the piglet breed is "Changbai" and a weaned piglet (purchased from Zhaoqing Dazhong pig farm) is used.
160 monitored 30-day-old 'growing-white' weaned piglets are purchased, weighed and numbered one by one, and randomly divided into 2 large groups according to a weight similarity principle, each large group comprises 4 repeated groups, and each repeated group comprises 20 piglets as a column so as to ensure that the average weight difference of each group is not significant.
One group is a control group, the control group is fed with feed containing hypoxanthine nucleotide and with weight content of 0.01%, the other group is a test group, and the test group is fed with feed containing hypoxanthine and with weight content of 0.01%.
The test period was 28 days during which drinking water was taken ad libitum. The feeding time is 8, 11, 14 and 17 points every day, and piglets in abnormal state are eliminated in time.
And recording the average value of the weight gain, the average value of daily feed intake, the average value of muscle weight gain, the average value of fat weight gain, the average value of 24h oxygen consumption and the average value of 24h respiratory entropy of piglets of the experimental group and the control group after being fed for 28 days.
And (3) putting the experimental group and the control group which are raised for 6 weeks into an environment at 4 ℃ for cold stimulation, and recording the average value of the body temperature reduction values of the experimental group and the control group in the environment at 4 ℃.
After the cold stimulation experiment is completed, performing cervical bloodletting to slaughter each experimental group and the control group, and recording the piglet liver weight average value and the spleen weight average value of the experimental group and the control group; and measuring the mean value of the hypoxanthine content in the blood of the piglets of the experimental group and the control group.
Example 18
The differences from example 17 are:
the experimental group was fed with a feed containing hypoxanthine in an amount of 0.08% by weight.
The control group was fed with a feed containing inosinic acid in an amount of 0.08% by weight.
Example 19
The differences from example 17 are:
the experimental group was fed with a feed containing hypoxanthine in an amount of 1.4% by weight.
The control group was fed with a feed containing inosinic acid at a weight content of 1.4%.
Example 20
The difference from example 17 is that:
the experimental group was fed with 2% by weight feed containing hypoxanthine.
The control group was fed with 2% by weight of a feed containing inosinic acid.
Example 21
The differences from example 17 are:
the experimental group was fed with a feed containing xanthine in an amount of 0.01% by weight.
The control group was fed with a feed containing 0.01% by weight of xanthine nucleotide. And measuring the mean value of the hypoxanthine content in the blood of the piglets of the experimental group and the control group.
Example 22
The difference from example 21 is that:
the experimental group was fed with a feed containing xanthine and having a weight content of 0.08%.
The control group was fed with a feed containing 0.08% by weight of xanthine nucleotide.
Example 23
The difference from example 21 is that:
the experimental group was fed with a feed containing 1.4% by weight of xanthine.
The control group was fed with a feed containing 1.4% by weight of xanthine nucleotide.
Example 24
The difference from example 21 is that:
the experimental groups were fed with 2% by weight feed containing xanthine.
The control group was fed with 2% by weight of feed containing xanthine nucleotide.
Example 25
The difference from example 17 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.01% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.01% by weight. And measuring the mean value of the hypoxanthine content in the blood of the piglets of the experimental group and the control group.
Example 26
The difference from example 25 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.08% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.08% by weight.
Example 27
The differences from example 25 are:
the experimental group was fed with a feed containing adenine in an amount of 1.4% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 1.4% by weight.
Example 28
The differences from example 25 are:
the experimental group was fed with a feed containing adenine in an amount of 2% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 2% by weight.
Example 29
The differences from example 17 are:
the experimental group was fed with a feed containing guanine in an amount of 0.01% by weight.
The control group was fed with a feed containing guanine nucleotide at a weight content of 0.01%. And measuring the mean value of the hypoxanthine content in the blood of the piglets of the experimental group and the control group.
Example 30
The difference from example 29 is that:
the experimental group was fed with a feed containing guanine in an amount of 0.08% by weight.
The control group was fed with a feed containing guanine nucleotide in an amount of 0.08% by weight.
Example 31
The difference from example 29 is that:
the experimental group was fed with 1.4% by weight feed containing guanine.
The control group was fed with 1.4% by weight of feed containing guanine nucleotide.
Example 32
The differences from example 29 are:
the experimental groups were fed with 2% by weight of feed containing guanine.
The control group was fed with a feed containing 2% by weight of guanine nucleotide. Specific experimental data for examples 17-32 are shown in tables 5-8
Table 5 shows experimental data for examples 17 to 20.
TABLE 5
Figure GDA0003907919680000131
Figure GDA0003907919680000141
Table 6 shows experimental data for examples 21 to 24.
TABLE 6
Figure GDA0003907919680000142
Table 7 shows the experimental data of examples 25 to 28.
TABLE 7
Figure GDA0003907919680000143
Figure GDA0003907919680000151
Table 8 shows the experimental data of examples 29 to 32.
TABLE 8
Figure GDA0003907919680000152
According to tables 5-8, when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the average daily feed intake and the average weight gain of the piglets are obviously improved compared with the condition that the piglets are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the effect of promoting the piglets to feed and then increasing the weight of the piglets and promoting the piglets to grow is obviously achieved when the purine is added into the feed;
when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the lean meat percentage of the piglets is increased compared with the condition that the piglets are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, the muscle increment of the piglets is increased and the fat increment is reduced as the initial physiological states of a plurality of piglets are close to each other, and the fact that the addition of the purine in the feed is obviously beneficial to increasing the muscle content of the piglets and enables the piglets to be stronger is proved;
when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the piglet feed has higher 24-hour feeding amount compared with the piglets fed with the feed added with the purine nucleotides such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the piglet feed has higher metabolism speed;
when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the average value of the 24-hour respiratory entropy of the piglets is closer to 1 than when the piglets are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, so that the piglets are proved to decompose a large amount of sugar when consuming energy, and the reduction of the blood sugar of the piglets is facilitated;
when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the average value of the 4 ℃ body temperature drop values of the piglets is reduced compared with the condition that the piglets are fed with the purine nucleotide such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, so that the piglets have better body temperature adjusting capability and better cope with cold stimulation;
when the piglets are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the weight average value of the livers and the weight average value of the spleens of the piglets are higher than that when the piglets are fed with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the initial physiological states of a plurality of piglets are close to each other, so that the internal organs of the piglets grow faster and better;
when a piglet is fed with a feed containing a purine such as inosine, xanthine, guanine and adenine, the average value of purine concentration in the serum of the piglet is higher than that when a piglet is fed with a feed containing a purine nucleotide such as inosinic acid, xanthosine, guanine nucleotide and adenine nucleotide, and the main reason for the effect is that the concentration of purine in the piglet is adjusted by taking purine from the feed, and the effects of promoting growth, increasing muscle content, reducing blood sugar, promoting metabolism, keeping body temperature more stably, promoting organ growth and the like are achieved.
Example 33
The hen breed in this example was yellow-feathered broilers (available from Sansanxian industries, ltd., guangzhou city).
After 20 hens of 30 days old are purchased and fed for one week, the hens are averagely divided into 2 groups according to the weight, each group contains 10 hens, one group is a control group, the other group is an experimental group, the control group is fed with feed containing hypoxanthine nucleotide and the weight content of the feed is 0.01%, and the experimental group is fed with feed containing hypoxanthine and the weight content of the feed is 0.01%; the breeding period is 3 weeks, the breeding is carried out at 26 deg.C in the whole day under light, and the people can eat and drink water freely.
And recording the weight gain mean value, daily feed intake mean value, muscle weight gain mean value, fat weight gain mean value, 24h oxygen consumption mean value and 24h respiratory entropy mean value of the hens of the experimental group and the control group after being fed for 3 weeks.
And (3) placing the experimental group and the control group which are raised for 3 weeks into an environment at 4 ℃ for cold stimulation, and recording the average value of the body temperature reduction values of the experimental group and the control group in the environment at 4 ℃.
After the cold stimulation experiment is completed, slaughtering each experimental group and each control group by bleeding the neck, and recording the liver weight average value and the spleen weight average value of each experimental group and each control group of hens; and measuring the mean value of the hypoxanthine content in the blood of the hens of each experimental group and the control group.
Example 34
The difference from example 33 is that:
the experimental group was fed with a feed containing hypoxanthine in an amount of 0.08% by weight.
The control group was fed with a feed containing inosinic acid in an amount of 0.08% by weight.
Example 35
The difference from example 33 is that:
the experimental group was fed with a feed containing hypoxanthine in an amount of 1.4% by weight.
The control group was fed with a feed containing inosinic acid at a weight content of 1.4%.
Example 36
The difference from example 33 is that:
the experimental group was fed with 2% by weight feed containing hypoxanthine.
The control group was fed with 2% by weight of a feed containing inosinic acid.
Example 37
The difference from example 33 is that:
the experimental group was fed with a feed containing xanthine and having a weight content of 0.01%.
The control group was fed with a feed containing 0.01% by weight of xanthine nucleotide. The mean values of the hypoxanthine contents in the blood of the hens in each experimental group and the control group were measured.
Example 38
The differences from example 37 are:
the experimental group was fed with a feed containing xanthine and having a weight content of 0.08%.
The control group was fed with a feed containing 0.08% by weight of xanthine nucleotide.
Example 39
The differences from example 37 are:
the experimental group was fed with a feed containing 1.4% by weight of xanthine.
The control group was fed with a feed containing 1.4% by weight of xanthine nucleotide.
Example 40
The difference from example 37 is that:
the experimental groups were fed with 2% by weight feed containing xanthine.
The control group was fed with 2% by weight of feed containing xanthine nucleotide.
Example 41
The difference from example 33 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.01% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.01% by weight. The mean values of the hypoxanthine contents in the blood of the hens in each experimental group and the control group were measured.
Example 42
The difference from example 41 is that:
the experimental group was fed with a feed containing adenine in an amount of 0.08% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 0.08% by weight.
Example 43
The difference from example 41 is that:
the experimental group was fed with a feed containing adenine in an amount of 1.4% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 1.4% by weight.
Example 44
The difference from example 41 is that:
the experimental group was fed with a feed containing adenine in an amount of 2% by weight.
The control group was fed with a feed containing adenine nucleotide in an amount of 2% by weight.
Example 45
The differences from example 33 are:
the experimental group was fed with a feed containing guanine and having a weight content of 0.01%.
The control group was fed with a feed containing guanine nucleotide at a weight content of 0.01%. And measuring the mean value of the hypoxanthine content in the blood of the hens of each experimental group and the control group.
Example 46
The difference from example 45 is that:
the experimental group was fed with a feed containing guanine in an amount of 0.08% by weight.
The control group was fed with a feed containing guanine nucleotide in an amount of 0.08% by weight.
Example 47
The difference from example 45 is that:
the experimental group was fed with 1.4% by weight of feed containing guanine.
The control group was fed with 1.4% by weight of feed containing guanine nucleotide.
Example 48
The difference from example 45 is that:
the experimental groups were fed with 2% by weight feed containing guanine.
The control group was fed with a feed containing 2% by weight of guanine nucleotide. Specific experimental data for examples 33-48 are shown in tables 9-12
Table 9 shows experimental data for examples 33 to 36.
TABLE 9
Figure GDA0003907919680000201
Table 10 shows the experimental data of examples 37 to 40.
Watch 10
Figure GDA0003907919680000202
Figure GDA0003907919680000211
Table 11 shows the experimental data of examples 41 to 44.
TABLE 11
Figure GDA0003907919680000212
Table 12 shows the experimental data of examples 45 to 48.
TABLE 12
Figure GDA0003907919680000213
Figure GDA0003907919680000221
As can be seen from tables 9 to 12, when the hens were fed with the feed containing the purine such as inosine, xanthine, guanine and adenine, the average daily food intake and the average weight gain of the hens were significantly improved as compared with the case where the hens were fed with the feed containing the purine such as inosinic acid, xanthine nucleotide, guanine nucleotide and adenine nucleotide, as can be seen from tables 1 to 4, and it was confirmed that the effect of promoting the hens to take food and thus increasing their weights and promoting their growth was significantly exerted when the feed containing the purine;
when the feed is fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the breast muscle rate of the hens is increased compared with the feed added with the purine nucleotide such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, the initial physiological states of a plurality of hens are close to each other, so that the muscle increment of the hens is increased and the fat increment is reduced, and the feed added with the purine is obviously beneficial to increasing the muscle content of the hens and enables the hens to be stronger;
when the feed such as hypoxanthine, xanthine, guanine and adenine is fed to the hen, the feed consumption of the hen for 24 hours is higher than that when the feed such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide is fed to the hen, and the metabolism speed of the hen is proved to be accelerated;
when the feed such as hypoxanthine, xanthine, guanine and adenine is fed to the hen, the average value of the 24-hour respiratory entropy of the hen is closer to 1 than when the feed such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide is fed to the hen, and therefore the feed proves that a large amount of sugar is decomposed when the hen consumes energy, and the blood sugar of the hen is favorably reduced;
when the feed containing the hypoxanthine, the xanthine, the guanine, the adenine and other purine nucleotides is fed to the hen, compared with the feed containing the hypoxanthine nucleotide, the xanthine nucleotide, the guanine nucleotide, the adenine nucleotide and other purine nucleotides, the average value of the 4 ℃ body temperature reduction value of the hen is reduced, and the feed proves that the hen has better capability of regulating the body temperature and better responds to cold stimulation;
when the hens are fed with the feed added with the purine such as hypoxanthine, xanthine, guanine and adenine, the liver weight mean value and the spleen weight mean value of the hens are higher than those of the hens fed with the feed added with the purine such as hypoxanthine nucleotide, xanthine nucleotide, guanine nucleotide and adenine nucleotide, and the internal organs of the hens grow faster and better as the initial physiological states of a plurality of hens are close to each other;
when a hen is fed with a feed containing a purine such as inosine, xanthine, guanine and adenine, the mean value of purine concentration in the serum of the hen is higher than when a hen is fed with a feed containing a purine nucleotide such as inosinic acid, xanthosine, guanine nucleotide and adenine nucleotide, and it is demonstrated that the main reason for the effect is that the concentration of purine in the hen is adjusted by ingesting purine from the feed, and the effect of promoting growth, increasing muscle content, lowering blood sugar, promoting metabolism, maintaining body temperature more stably, promoting organ growth and the like is exerted.
The embodiments of the present invention are all preferred embodiments of the present invention, and the scope of the present invention is not limited thereby, so: equivalent changes made according to the structure, shape and principle of the invention shall be covered by the protection scope of the invention.

Claims (1)

1. The application of purine is characterized in that: the purine is added into the feed, comprises one or more of hypoxanthine, xanthine, adenine and guanine, the mass content of the adenine in the feed is 0.01% -0.08%, and the mass content of the hypoxanthine, xanthine and guanine in the feed is 0.01% -2%.
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