CN102973659A - Small complex prescription of traditional Chinese medicine for preventing and treating glucocorticoids-induced osteoporosis, and preparation method thereof - Google Patents
Small complex prescription of traditional Chinese medicine for preventing and treating glucocorticoids-induced osteoporosis, and preparation method thereof Download PDFInfo
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- CN102973659A CN102973659A CN2011102598603A CN201110259860A CN102973659A CN 102973659 A CN102973659 A CN 102973659A CN 2011102598603 A CN2011102598603 A CN 2011102598603A CN 201110259860 A CN201110259860 A CN 201110259860A CN 102973659 A CN102973659 A CN 102973659A
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Abstract
The invention provides a small complex prescription of a traditional Chinese medicine for preventing and treating glucocorticoids-induced osteoporosis, and a preparation method thereof. The complex prescription of the traditional Chinese medicine is composed of epimedium brevicornum maxim and ligustrum lucidum ait. Animal experiments demonstrate that the small complex prescription of the traditional Chinese medicine can effectively reduce the content of serum tartrate-resistant acid phosphatase and alkaline phosphatase, increase serum osteocalcin and the calcium content of bone tissues, and effectively improve pathological changes of the bone tissues, in a hormone-intervened wistar osteoporosis model. The small complex prescription of the traditional Chinese medicine is based on neutrally supplementing yin and yang in kidney with tonifying kidney and strengthening bones as treatment principles, can effectively prevent and alleviate adverse effects of osteoporosis caused by taking glucocorticoids for a long time, provide effective preparations for preventing and treating osteoporosis to other varieties of patients suffering from osteoporosis, and has good development and application prospects.
Description
Technical field:
The invention belongs to medical technical field, be specifically related to a kind of tcm small compound prescription of preventing and treating glucocorticoid-induced osteoporosis and preparation method thereof.
Background technology:
Glucocorticoid (glucocorticoid, GC) is important and effective immunosuppressant and antiphlogistic effects are widely used in the fields such as anti-transplantation immunity repulsion, autoimmune disease, infection and oncotherapy owing to it.But, accept for a long time the patient that GC treats, surpass 50% bone loss can occur, it is that secondary osteoporosis also is the topmost reason of iatrogenic osteoporosis, its incidence rate is about 30%-50%, and GC dosage is larger, the course for the treatment of is longer, and the incidence rate of spontaneous fracture is just higher.Therefore, effectively prevent and treat this iatrogenic osteopathia, the major issue that sufficient modern medicine needs to be resolved hurrily has become multi-disciplinary, research one of a most active problem.For glucocorticoid-induced osteoporosis (glucocorticoid-induced osteoporosis, GIOP) control, doctor trained in Western medicine does not have ideal medicament at present, the treatment of primary osteoporosis is often used for reference in clinical application, the most scholar advocates to unite the sharp vitamin D of use calcium preparation, but system evaluation thinks that late result (after 36 months) is not remarkable, and has little significance to forming osteoporotic patient.This is because negative calcium balance is one of pathogenesis of GIOP.
In view of modern medicine does not have the medicine of good control GIOP, many doctors family turns to TCM Therapy to eye.Theory of Chinese medical science thinks that kidney storing essence master bone is given birth to marrow.Kidney essense is enriched then medullization and is given birth to actively, and bone gets it and supports then firm strong: if deficiency of kidney-essence then medullization give birth to weary source, bone loses it and supports and the skeleton fragility will occur, lumbar and back pain, the symptoms such as shin knees soreness.Chinese medicine thinks that hormone is the product of exogenous " pure sun ", " pathogenic heat ", cause kidney to lose the envelope Tibetan after acting in a large number for a long time human body, the yin and yang imbalance of kidney, the kidney essense consume, deficiency of kidney-essence is difficult to enrich skeleton, bone loses to moisten and supports and the formation of formation osteoporosis, belongs to the traditional Chinese medical science " atrophic debility of bones of suffering from a deficiency of the kidney ", " rheumatism involving the bone " category.Say as " Plain Questions flaccidity opinion ": " the kidney being in charge of bone and marrow ... heat in the kidney, failure of the spinal column to keep in upright position then, bone is withered and marrow subtracts, and sends out to be the atrophic debility of bones ".Therefore, the pathogenesis of GIOP is that the Tibetan is sealed in the kidney mistake, deficiency of kidney-essence, and the empty mistake of bone is foster, controls the method for suitable kidney tonifying, essence replenishing bone strengthening.
Chinese medicine is only controlled from general " kidney governing bones " opinion primary disease at present, does not illustrate its pathogenesis particularity, and its method for the treatment of and primary osteoporosis are without significant difference, so curative effect is not rational.GIOP is the osteoporosis of secondary on protopathy disease basis, belong to complicated disease, when clinical application, often to consider the treatment of protopathy, again according to the different syndromes of its hormon consumption performance in period, identification of etiology according to differenciation of symptoms and signs is asked machine, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs alleviates the hormone untoward reaction to reach, and increases the purpose of curative effect.Certainly need note abiding by the law defence treatment, because primary disease is great chronic disease, the medicine produce effects needs by quantitative change to qualitative change, in case firmly believe pathogenesis, the random side's of change medicine.Therefore, method for the treatment of and the medicine sought for GIOP have important practical significance.The effective formula and drug of the Chinese medicine prevention GIOP Chinese pathology mechanism that theory of Chinese medical science is probed into GIOP of must combining closely, and modern medicine furthers investigate the pathogenetic understanding of GIOP is for further clinical research provides experimental basis.
Summary of the invention:
The technical problem to be solved in the present invention is to overcome above-mentioned weak point, and the Chinese and western medicine theory of combining closely is to the understanding of the pathomechanism of GIOP, the effective formula and drug of research control GIOP.
1. the object of the invention provides a kind of tcm small compound prescription of preventing and treating glucocorticoid-induced osteoporosis and preparation method thereof.
2. pharmaceutical composition of the present invention (tcm small compound prescription) is comprised of following weight proportion Chinese medicine: Herba Epimedii 12-30g, Fructus Ligustri Lucidi 6-20g.
3. the used crude drug source of the present invention meets 2010 editions pertinent regulations of the Pharmacopoeia of the People's Republic of China.
Herba Epimedii: the dry aerial parts of Berberidaceae plant Herba Epimedii Epimedium brevicornum Maxim.
Fructus Ligustri Lucidi: the dry mature fruit of Oleaceae plants Fructus Ligustri Lucidi Ligustrum lucidum Ait..
4. another object of the present invention provides a kind of preparation method of the compound Chinese medicinal preparation for preventing and treating GIOP, and the main method of the party comprises the following steps:
(1) decocting: take by weighing dry medical material according to the prescription proportioning, add 6-12 times of water gaging according to the medical material gross weight, soak 30-90min, decoct 2-3 time, each 30min-1hr.Collecting decoction also filters, concentrates;
(2) precipitate with ethanol: above-mentioned decocting liquid is let cool to room temperature, add ethanol and make and contain alcohol amount and reach 50-70%, cold preservation is spent the night; Get the supernatant Recycled ethanol;
(3) the gained medicinal liquid is evaporated to every milliliter and contains the 1-1.5g crude drug, or to dry extract, it is for subsequent use to be ground into fine powder;
(4) with extract powder and pharmaceutic adjuvant in 1: the ratio of 1-3 is mixed granule processed, granulate, dry granule; With the encapsulated capsule that gets of granule; Adding 0.5% just fatty acid magnesium tabletting gets tablet; To be evaporated to the concentrated solution of every milliliter of 1-1.5g crude drug, fill gets oral liquid.
5. a further object of the invention has provided the application of above-mentioned Chinese medicine preparation in preparation control glucocorticoid-induced osteoporosis medicine.
The inventor has carried out pharmacodynamic experiment research to preparation of the present invention, observes glucocorticoid dexamethasone in treatment OVA and causes the change of bone metabolism related biochemical indicator of Rat Asthma Model and Herba Epimedii Fructus Ligustri Lucidi to its influence.The result shows, compare with Normal group, glucocorticoid is intervened Rat Asthma Model and is existed remarkable reduction, serum Tartrate resistant acid phosphatase (TRAP) and the alkali phosphatase (ALP) of serum osteocalcin (BGP), osseous tissue calcium ion level significantly to raise, osteoporosis sample pathological change appears in femur, and the hyperfunction abnormal bone metabolism of bone resorption the bone formation inhibition appearred and in Rat Asthma Model behind the prompting glucocorticoid therapeutic intervention.After giving the treatment of Herba Epimedii Fructus Ligustri Lucidi; serum BGP and osseous tissue calcium ion level obviously raise; TRAP and ALP level obviously reduce, and effectively improve bone pathology variation etc., and prompting Chinese medicine causes unusually playing a protective role of bone metabolism to the hormone therapeutic intervention.In addition, depend on because of the performance of hormonal action and to transcribe, lung tissue of rats has been carried out the detection of transcription factor chip, found that, hormone causes osteoporotic relevant transcription factor to it, mainly comprise sex hormone receptor (androgen receptor AR, estrogen receptor ER, progesterone receptor PR), vit D3 receptor (VDR), Apoptosis is (such as Bcl-2, P53, c-Myb etc.) protein expression all has the effect of remarkable downward modulation, the impact of prompting transcriptional level may be that hormone causes osteoporotic important step, and after the intervention of Herba Epimedii Fructus Ligustri Lucidi, These parameters all is significantly improved effect.
Advantage of the present invention
(1) Chinese medicine preparation of the present invention is for the characteristics of GIOP pathogenesis it " deficiency of kidney-essence ", selecting the medicine of negative and positive in the flat the kidney invigorating is object of study to the Herba Epimedii Fructus Ligustri Lucidi, provides the basis for developing flat nourishing YIN sun medicine to the new drug of Herba Epimedii Fructus Ligustri Lucidi osteoporosis.
(2) letter of the composition medicine of preparation of the present invention is and smart, and consumption is moderate, and curative effect is sure, taking convenience.
Specific implementation method
Example 1
Herba Epimedii 15kg, Fructus Ligustri Lucidi 15kg
With Herba Epimedii 15kg, Fructus Ligustri Lucidi 15kg mixes, and adds 240kg water soaking 30min, decocts 2 times, each 1hr.Merge 2 decocting liquids and be concentrated into the extractum of relative density 1.15-1.20.Adding 95% ethanol makes it contain alcohol amount to reach 60%.Hold over night is got the supernatant decompression recycling ethanol, and to be concentrated into relative density be 1.3 extractum, and drying under reduced pressure is ground into fine powder.Extract powder 2.4kg is mixed according to 1: 1 ratio with dextrin 2.4kg, make soft material, cross the sieve series granule, 70 ℃ of constant pressure and dry 4h are by every bag 0.5g.
Example 2
Herba Epimedii 12kg, Fructus Ligustri Lucidi 8kg
With Herba Epimedii 12kg, Fructus Ligustri Lucidi 8kg mixes, and adds 160kg water soaking 30min, decocts 2 times, each 1hr.Merge 2 decocting liquids and be concentrated into the extractum of relative density 1.15-1.20.Adding 95% ethanol makes it contain alcohol amount to reach 60%.Hold over night is got the supernatant decompression recycling ethanol, and to be concentrated into relative density be 1.3 extractum, and drying under reduced pressure is ground into fine powder.Extract powder 1.5kg is mixed according to 1: 1 ratio with dextrin 1.5kg, make soft material, cross the sieve series granule, 70 ℃ of constant pressure and dry 4h, granulate, encapsulated, every dress 0.5g.
Example 3
Herba Epimedii 12kg, Fructus Ligustri Lucidi 8kg
With Herba Epimedii 12kg, Fructus Ligustri Lucidi 8kg mixes, and adds 160kg water soaking 30min, decocts 2 times, each 1hr.Merge 2 decocting liquids and be concentrated into the clear paste of relative density 1.15-1.20.Adding 95% ethanol makes it contain alcohol amount to reach 60%.Hold over night is got the supernatant decompression recycling ethanol, and to be concentrated into relative density be 1.3 extractum, and drying under reduced pressure is ground into fine powder.Extract powder 1.5kg is mixed according to 1: 1 ratio with dextrin 1.5kg, make soft material, cross the sieve series granule, 70 ℃ of constant pressure and dry 4h add 0.5% magnesium stearate mixing, with tabletting behind the sieve granule on the 14th, and every heavy 0.4g.
Example 4
Herba Epimedii 30kg, Fructus Ligustri Lucidi 20kg
With Herba Epimedii 30kg, Fructus Ligustri Lucidi 20kg mixes, and adds 400kg water soaking 30min, decocts 2 times, each 1hr.Merge 2 decocting liquids and be concentrated into the clear paste of relative density 1.15-1.20.Adding 95% ethanol 100kg makes it contain alcohol amount to reach 60%.Hold over night is got the supernatant decompression recycling ethanol, and is concentrated into 50000ml, filter, and fill, every 10ml, sterilization namely gets oral liquid.
Claims (3)
1. a Chinese medicine preparation that is used for the control glucocorticoid-induced osteoporosis is characterized in that said preparation contains the Chinese medicine composition of following weight proportion: Herba Epimedii 12-30g, Fructus Ligustri Lucidi 6-20g.
2. a preparation method of preventing and treating as claimed in claim 1 the Chinese medicine preparation of glucocorticoid-induced osteoporosis is characterized in that the method comprises the following steps:
(1) decocting: add 6-12 times of water gaging according to the medical material gross weight, soak 30-90min, decoct 2-3 time, each 30min-1hr, collecting decoction also filters, concentrates;
(2) precipitate with ethanol: above-mentioned decocting liquid is let cool to room temperature, add ethanol and make and contain alcohol amount and reach 50-70%, cold preservation is spent the night; Get the supernatant Recycled ethanol;
(3) the gained medicinal liquid is evaporated to every milliliter and contains the 1-1.5g crude drug, or to dry extract, it is for subsequent use to be ground into fine powder;
(4) extract powder and pharmaceutic adjuvant are prepared tablet, capsule, granule or oral liquid.
3. the Chinese medicine composition application in preparation protect against osteoporosis medicine is characterized in that this Chinese medicine composition is the preparation that the raw material of Chinese medicine by following weight proportion becomes to be grouped into: Herba Epimedii 12-30g, Fructus Ligustri Lucidi 6-20g.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103768158A (en) * | 2014-01-26 | 2014-05-07 | 首都医科大学 | Pharmaceutical composition for preventing and treating osteoporosis and preparation method thereof |
| CN105748603A (en) * | 2016-04-07 | 2016-07-13 | 杨柳 | Traditional Chinese medicine preparation for preventing and treating osteoporosis and preparation method of traditional Chinese medicine preparation |
| CN110051731A (en) * | 2019-05-15 | 2019-07-26 | 首都医科大学 | A kind of Chinese medicine composition of anti-aging and preparation method thereof |
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| US20030170292A1 (en) * | 2001-11-09 | 2003-09-11 | National University Of Singapore | Methods for preparing an estrogenic preparation and isolated estrogenic compounds from a plant and uses thereof |
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Patent Citations (4)
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| US20030170292A1 (en) * | 2001-11-09 | 2003-09-11 | National University Of Singapore | Methods for preparing an estrogenic preparation and isolated estrogenic compounds from a plant and uses thereof |
| JP2003313137A (en) * | 2002-04-19 | 2003-11-06 | Bamen Kenichi | Composition for treating osteoporosis |
| CN1709423A (en) * | 2005-06-06 | 2005-12-21 | 广东医学院 | Medicinal formulation of glucocorticoid and Chinese medicine for preventing and treating asthma and COPD |
| JP2010535815A (en) * | 2007-08-08 | 2010-11-25 | バイオノボ・インコーポレーテッド | Estrogenic extract of oleaceae and its use |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103768158A (en) * | 2014-01-26 | 2014-05-07 | 首都医科大学 | Pharmaceutical composition for preventing and treating osteoporosis and preparation method thereof |
| CN105748603A (en) * | 2016-04-07 | 2016-07-13 | 杨柳 | Traditional Chinese medicine preparation for preventing and treating osteoporosis and preparation method of traditional Chinese medicine preparation |
| CN105748603B (en) * | 2016-04-07 | 2019-06-28 | 黑龙江中医药大学 | A kind of Chinese materia medica preparation and preparation method thereof of anti-curing osteoporosis |
| CN110051731A (en) * | 2019-05-15 | 2019-07-26 | 首都医科大学 | A kind of Chinese medicine composition of anti-aging and preparation method thereof |
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Application publication date: 20130320 |