CN102972278A - Method for cultivating brassica chinensis to bloom indoors with LED light source - Google Patents
Method for cultivating brassica chinensis to bloom indoors with LED light source Download PDFInfo
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- CN102972278A CN102972278A CN2012105884211A CN201210588421A CN102972278A CN 102972278 A CN102972278 A CN 102972278A CN 2012105884211 A CN2012105884211 A CN 2012105884211A CN 201210588421 A CN201210588421 A CN 201210588421A CN 102972278 A CN102972278 A CN 102972278A
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Abstract
The invention discloses a method for cultivating brassica chinensis to bloom indoors with an LED light source and belongs to the field of biotechnology. The method comprises the following steps: after germination for 2-3 days, seeds of the brassica chinensis sprout white buds and then are sown in a nutrition pot which is 25 cm in height and 12 cm in width, wherein vermiculite and grass carbon as the substrate at the ratio of 1V:1V are loaded in the nutrition pot; and after 7-8 days, seed leaves are flattened, and the brassica chinensis seedlings are cultivated under a light-emitting diode (LED) with monochrome red light and blue-red combination light at the ratio of 2:7 for 90 days, and a light source with monochrome red light and blue-red combination light at the ratio of 2:7 is most suitable for brassica chinensis to bloom. The method has the advantages of simplicity of operation, short blooming time and excellent fertility of plants, energy conservation and environmental protection, and the like, and is applied to the field of indoor quick breeding, industrialized production of breeds and the like.
Description
Technical field
The present invention relates to a kind of method of using the little green vegetables of the indoor cultivation of led light source to bloom, belong to biological technical field.
Background technology
Green vegetables are one of vegetables with green leaves of the main cultivation of south China and happiness food.But present little green vegetables Breeding Situation allows of no optimist, and the means of breeding are backward, yield potential is low, resistance and that quality is undesirable, germplasm is degenerated is fast.Carry out the cultivation of blooming of little green vegetables at the controlled indoor employing led light source of environment, can break the season limit of little green vegetables breeding work, can make little green vegetables whenever blooming in a year, be conducive to crossbreeding and the reproductive study of little green vegetables.
Photaesthesia is because in the plant a series of light receptor is arranged in light quality, light intensity, light to reaching the photoperiod.Photomorphogenesis be the differentiation that relies on the photocontrol cell, 26S Proteasome Structure and Function change, finally accumulate building up of tissue and organ, also claim the photocontrol growth course.It is the reaction of a low energy, and light promotes series reaction in the cell as ambient signal deexcitation light receptor, finally shows as the variation of morphosis.Along with the progress of science and technology and the fast development of agricultural and biological industry, plant has broken through the restriction of simple dependence sunshine to the utilization of efficient light, and artificial light source replaces or the deficiency of replenishing lamp has become the important means of environment control growth and development of plants.Artificial light source in agricultural and biological field use mainly contains fluorescent lamp, incandescent lamp, sodium vapor lamp and dysprosium lamp etc. for a long time, wherein high-pressure mercury lamp and dysprosium lamp are luminous efficiency and the higher light source of efficient light combined coefficient, but power consumption is large, emission spectrum can not be well matches with the photosynthesis absorption spectrum of plant, and it is low to be used as light filling efficient; And produce a lot of thermal radiation, and can not approach irradiation to plant, not high to plant growth light stimulus efficient, it is very high to be used as the artificial light source cost.Therefore, introduce that energy-conservation, good luminous performance, quantity of heat production are low, the new type light source of long service life, to improve utilization rate of electrical in the plant factor and space availability ratio, reduction power consumption cost is significant.
Light emitting diode (Light Emitting Diodes, LED) as the 4th generation the novel illumination light source, have that energy-saving and environmental protecting, photoelectric transformation efficiency are high, the life-span is long, heating is low, cooling load is little, light quantity and light quality can regulate, be easy to disperse or make up many important feature that are different from other electric light sources such as control.Because these significant features, the plant that LED is fit to be applied in the controllable environment is cultivated or cultivation, such as Plant Tissue Breeding, facilities horticulture and factorial seedling growth and the ecological life support system of space flight etc.Compare with artificial light sources such as incandescent lamp, fluorescent lamps, the advantage of LED is: (1) uses low, the efficient energy-saving of supply voltage; (2) can send the narrower monochromatic light of light wave; (3) cold light source; (4) can within the extremely short time, send pulsed light; (5) volume is little, and stability is strong, and the response time is fast; (6) environmentally safe; (7) compact conformation and life-span are long.In the situation of same lighting effect, LED power consumption is 1/8th of incandescent lamp, is 1/2nd of fluorescent lamp, to energy savings, to reduce greenhouse effect very favourable.Simultaneously, LED can closely shine plant as cold light source, will greatly improve the utilization ratio in space.Compare with the artificial light source that high-pressure mercury lamp, high-pressure sodium lamp, metal halide lamp etc. contain mercury metal with fluorescent lamp, LED is the total solids illuminator, and is not mercurous, shock-resistant, is difficult for fragmentation, and the discarded object recoverable is a kind of free of contamination green light source.In addition, can reach more than 50, the 000h service life of LED, is the decades of times of ordinary light source.Therefore, the plant that the light source that utilizes the feature development of LED to go out is applicable in the controllable environment is cultivated or cultivation.
Summary of the invention
The purpose of this invention is to provide a kind of scheme of using the little green vegetables of the indoor cultivation of led light source to bloom, for the scientific research of a variety of Chinese cabbage provides energy-conservation, efficient platform.
Technical scheme
A kind of method of using the little green vegetables of the indoor cultivation of led light source to bloom comprises following content:
1. the preparation of seedling: select little green vegetables seed and will select purity, cleanliness good, germination rate is high, full seed.For promoting seed sprouting, also need with 30 ℃ clean clear water seed to be eluriated 2-3 time first, soaked seed 3-4 hour.After seed soaking finishes seed is eluriated 2-3 time again, drained excessive moisture, trying to get to the heart of a matter at the plastics seedling spreads 2 layers of old newspaper or blotting paper is sowed vernalization.Seed after showing money or valuables one carries unintentionally in vernalization 2-3 days, and live to enter to be equipped with matrix be that (1V: in nutritive cube 1V) (high 25 cm * wide 12 cm), cotyledon flattens vermiculite+peat composed of rotten mosses after 7-8 days.
2. light treatment: the little green vegetables seedling that cotyledon flattens is positioned under the led light source and cultivated 90 days.
3. the condition of light treatment:
1. light source is monochromatic ruddiness and Lan Hong group 2:7;
2. light intensity is 150 μ molm
-2S
-1
3. the photoperiod is 12 hours;
4. cultivation temperature 22-24 ℃.
4. control method:
1. described light intensity is to control to the distance of influences of plant crown by quantity and the lamp of adjusting lamp;
2. the described photoperiod adopts a timing means to control.
5. little green vegetables seed is the little green vegetables of heat resistanceheat resistant type.
Beneficial effect
The invention provides a kind of method of using the little green vegetables of the indoor cultivation of led light source to bloom.Culture technique of the present invention has the following advantages: 1) simple to operate, effective; 2) plant blossom is fast, many, and fertility is good; 3) save energy environment protection.The inventive method and result provide reliable technical scheme for the indoor breeding of little green vegetables, also provide good platform for the relevant Olericulture research of little green vegetables.
Description of drawings
Fig. 1, chlorophyll content is significantly higher than the fluorescent lamp processing under monochromatic ruddiness and the Lan Hong combined light.
Fig. 2, soluble protein is apparently higher than control treatment under the blue red combined light.
Fig. 3, ascorbic acid is significantly higher than control treatment under monochromatic ruddiness and the Lan Hong combined light.
Embodiment
Below by embodiment the present invention is further described in detail.
Method therefor is conventional method if no special instructions among the following embodiment, and described percentage composition is volumn concentration if no special instructions.
1. enforcement material:
1. little green vegetables seed: heat resistanceheat resistant 605(produces with planting).
2. light source: light emitting diode (LED).
2. implementation method:
1. the preparation of seedling: little green vegetables seed after showing money or valuables one carries unintentionally in vernalization 2-3 days, and live to enter to be equipped with matrix be that (1V: in nutritive cube 1V) (high 25 cm * wide 12 cm), cotyledon flattens vermiculite+peat composed of rotten mosses after 7-8 days.
2. light treatment: the little green vegetables seedling that cotyledon flattens is positioned over to be cultured under the LED and blooms.
3. condition control: light source is monochromatic ruddiness and Lan Hong combination 2:7, and light intensity is 150 μ molm
-2S
-1, the photoperiod is 12 hours, temperature 22-24 ℃, incubation time is 90 days.
4. control method: provide light source by LED light lamp, control light intensity by quantity and the lamp of adjusting lamp to the distance of influences of plant crown, a timing means is controlled the photoperiod.
3. treatment effect:
1. different light medium is on the bloom impact of process of little green vegetables
The number number under ruddiness and Lan Hong combination 2:7 of blooming of little green vegetables is maximum, is significantly higher than the fluorescent lamp contrast.Lan Hong combination and monochromatic ruddiness are conducive to bloom (table 1) of little green vegetables.
2. different light medium is on the impact of little green vegetables Photosynthetic Pigment
The chlorophyll a of little green vegetables blade, chlorophyll b, chlorophyll total amount and carotenoid content are maximum under Lan Hong combination 2:7, contrast apparently higher than fluorescent lamp.Blue red combined light is conducive to the accumulation (Fig. 1) of little green vegetables photosynthetic pigment.
3. different light medium is on the impact of little green vegetables leaf soluble protein content
The soluble protein content of little green vegetables is maximum under Lan Hong combination 2:7 light, is monochromatic ruddiness secondly, and the fluorescent lamp contrast is lower minimum.Lan Hong combination and monochromatic ruddiness are conducive to the accumulation (Fig. 2) of little green vegetables leaf soluble protein content.
4. different light medium is on the impact of little green vegetables blade ascorbic acid (Vc)
The ascorbic acid content of little green vegetables is maximum under Lan Hong combination 2:7 and monochromatic ruddiness.Lan Hong combination and monochromatic ruddiness are conducive to the accumulation (Fig. 3) of little green vegetables blade ascorbic acid.
Table 1
Claims (4)
1. method of using the little green vegetables of the indoor cultivation of led light source to bloom comprises following content:
1. the preparation of seedling: little green vegetables seed after showing money or valuables one carries unintentionally in vernalization 2-3 days, and live to enter to be equipped with matrix be vermiculite+peat composed of rotten mosses (1V: in nutritive cube 1V) (high 25 cm * wide 12 cm), be cultured to cotyledon and flatten;
2. light treatment: the little green vegetables seedling that cotyledon flattens is positioned under the led light source and cultivated 90 days.
2. method according to claim 1 is characterized in that, the condition of described light treatment:
1. light source is monochromatic ruddiness and Lan Hong combined light 2:7;
2. light intensity is 150 μ molm
-2S
-1
3. the photoperiod is 12 hours;
4. cultivation temperature 22-24 ℃.
According to claim 1 with method claimed in claim 2, it is characterized in that:
1. described light intensity is to control to the distance of influences of plant crown by quantity and the lamp of adjusting lamp;
2. the described photoperiod adopts a timing means to control.
4. method according to claim 1 is characterized in that, described little green vegetables are the heat resistanceheat resistant type.
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| CN2012105884211A CN102972278B (en) | 2012-12-31 | 2012-12-31 | Method for cultivating brassica chinensis to bloom indoors with LED light source |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222420A (en) * | 2013-04-28 | 2013-07-31 | 南京农业大学 | Small vegetable indoor cultivation technique based on LED energy saving light source |
| CN103960111A (en) * | 2014-05-05 | 2014-08-06 | 安徽科技学院 | Indoor production technology for water planted sonchus oleraceus |
| CN105766338A (en) * | 2016-03-30 | 2016-07-20 | 安徽科技学院 | Seedling culture method of Abelmoschus esculentus |
| CN105766587A (en) * | 2016-03-30 | 2016-07-20 | 安徽科技学院 | Seedling breeding method of abelmoschus esculentus |
| CN106171395A (en) * | 2016-07-08 | 2016-12-07 | 周智 | A kind of method utilizing artificial light type plant factor plantation Plantula Brassicae chinensis |
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| CN101889531A (en) * | 2010-06-28 | 2010-11-24 | 南京农业大学 | Method for controlling indoor seedling raising of upland cotton |
| CN102388800A (en) * | 2011-07-26 | 2012-03-28 | 南京农业大学 | Light source control method for tissue culture of brassica napus |
| CN102415300A (en) * | 2011-08-02 | 2012-04-18 | 北京林业大学 | Application of LED (Light Emitting Diode) in plant cultivation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222420A (en) * | 2013-04-28 | 2013-07-31 | 南京农业大学 | Small vegetable indoor cultivation technique based on LED energy saving light source |
| CN103222420B (en) * | 2013-04-28 | 2014-11-26 | 南京农业大学 | Small vegetable indoor cultivation technique based on LED energy saving light source |
| CN103960111A (en) * | 2014-05-05 | 2014-08-06 | 安徽科技学院 | Indoor production technology for water planted sonchus oleraceus |
| CN105766338A (en) * | 2016-03-30 | 2016-07-20 | 安徽科技学院 | Seedling culture method of Abelmoschus esculentus |
| CN105766587A (en) * | 2016-03-30 | 2016-07-20 | 安徽科技学院 | Seedling breeding method of abelmoschus esculentus |
| CN106171395A (en) * | 2016-07-08 | 2016-12-07 | 周智 | A kind of method utilizing artificial light type plant factor plantation Plantula Brassicae chinensis |
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