CN102972203A - Inoculation operation method for reducing contamination rate of cultivating bottle - Google Patents
Inoculation operation method for reducing contamination rate of cultivating bottle Download PDFInfo
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- CN102972203A CN102972203A CN2012103166851A CN201210316685A CN102972203A CN 102972203 A CN102972203 A CN 102972203A CN 2012103166851 A CN2012103166851 A CN 2012103166851A CN 201210316685 A CN201210316685 A CN 201210316685A CN 102972203 A CN102972203 A CN 102972203A
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- shower nozzle
- alcohol
- liquid spawn
- conveyance conduit
- culture bottle
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Abstract
The invention relates to the field of bioengineering, in particular to a strain inoculation operation method. The inoculation operation method for reducing the contamination rate of a cultivating bottle comprises the following steps: 1) strain spraying, 2 cleaning and degerming, 3) waste water treatment. By the adoption of the technique, the inoculation operation method for reducing the contamination rate of the cultivating bottle is simple in structure, low in constructing cost, and capable of effectively cleaning and degerming a liquid strain inoculation device, reducing the contamination rate of liquid strains, and improving rate of survival of strains.
Description
Technical field
The present invention relates to a kind of bioengineering field, particularly a kind of bacterial classification inoculation method of operating.
Background technology
Edible mushroom is one of food that is of high nutritive value of liking of people, and the edible mushroom artificial cultivation technique has very large development in recent years, and the growing and cultivation technology of edible mushroom is also gradually from the manually plantation in past to automation, batch production development.And in the planting process of edible mushroom, the inoculating process of edible fungus species directly plays vital impact to the growth of edible mushroom.
At present; the bacterial classification of edible mushroom mainly be divided into solid spawn and and liquid spawn; wherein; the relative solid spawn of liquid spawn has short, fast, the advantage such as biological transformation ratio is high, bacterial classification purity height of bacterium of incubation time; and the scale of being more convenient for, continuously production are the trend of mushroom industry batch production Development Technology innovation.But, after using the liquid spawn conveyance conduit to carry a period of time, become easily infected by bacterium on the shower nozzle of liquid spawn conveyance conduit, and the solution of liquid spawn contains abundant nutrient component, in case bacterial adsorption is after on the shower nozzle, can carry out Fast-propagation by the solution of being infected with the liquid spawn on shower nozzle, the liquid spawn of exporting out from the liquid spawn conveyance conduit is directly polluted, and after with this liquid spawn culture bottle being inoculated, except culture bottle having been caused two degree pollutions, also can affect the quality of postvaccinal mycelia, reduce the output of edible mushroom.
Summary of the invention
The object of the invention is to, a kind of inoculation method of operating that reduces the culture bottle pollution rate is provided, solve above technical problem.
Technical problem solved by the invention can realize by the following technical solutions:
Reduce the inoculation method of operating of culture bottle pollution rate, it is characterized in that, comprise the steps:
1) bacterial classification sprays: the seeding tank that liquid spawn is housed is inflated, make the liquid spawn seeding tank of gushing out, liquid spawn is from exporting the shower nozzle that the liquid spawn conveyance conduit is connected to the tank connected liquid spawn conveyance conduit of seed, and described shower nozzle is sprayed to liquid spawn in the culture bottle.
2) clean degerming: liquid spawn is injected sterile water to described liquid spawn conveyance conduit after spraying and finishing, and described shower nozzle is washed degerming, and described shower nozzle is carried out alcohol disinfecting.
3) wastewater treatment: the sterile water that sprays in the described shower nozzle is sprayed to be used to the workbench of putting culture bottle, and the waste water after spraying is collected discharging.
After bacterial classification sprays, the solution of meeting residual liquid bacterial classification on the described shower nozzle, described solution contains abundant nutriment, easily by airborne bacteria adherence on shower nozzle, and carry out Fast-propagation by residual solution, liquid spawn to follow-up sprinkling is caused germ contamination, and the present invention is after each the sprinkling, utilize sterile water to wash described shower nozzle and liquid spawn conveyance conduit, both prevented that described liquid spawn conveyance conduit and shower nozzle from stopping up, the bacterium on the shower nozzle can have been washed away cleaning again, reduced bacterium to the pollution of liquid spawn, pollute thereby avoid culture bottle to be subject to two degree, thereby guarantee that bacterial classification can be quick after entering culture bottle, healthy growth.
In described liquid-spawn inoculation process, implementation step 1 repeatedly) at least after half an hour, carry out one time step 2), off time between twice step 1) in succession implementing is for implementing a step 2) operating time, carry out liquid spawn when inoculating at every turn, all need seeding tank such as is inflated at the preparation, this is just so that can cause off time in twice liquid-spawn inoculation implementation process, and described step 2) within described off time, implement, and with step 2) the enforcement time be controlled at after for the first time liquid-spawn inoculation is implemented implement to the second liquid bacterial classification inoculation before between, the availability of equipment can be taken full advantage of, the liquid-spawn inoculation whole efficiency can be improved again.
Step 2) in, described liquid spawn conveyance conduit injects before the sterile water, first described seeding tank and liquid spawn conveyance conduit is disconnected, and stops described sterile water to flow in the described seeding tank.
Be provided with a magnetic valve between described seeding tank and the described liquid spawn conveyance conduit, described magnetic valve is the bacterial classification magnetic valve, the control end of described bacterial classification magnetic valve connects a microprocessor system, by the described bacterial classification magnetic valve of opening and closing, realize described seeding tank and liquid spawn conveyance conduit connected sum are disconnected.
Step 2) in, the flow control of described sterile water is between 0.5 metre per second (m/s) to 3 metre per second (m/s), in order to described liquid spawn conveyance conduit and shower nozzle are formed scouring effect, carry the sterile water conveyance conduit of sterile water between described seeding tank and described shower nozzle, described sterile water conveyance conduit is communicated with described liquid spawn conveyance conduit, the junction of described sterile water conveyance conduit and described liquid spawn conveyance conduit is provided with another magnetic valve, described magnetic valve is the water delivery magnetic valve, the control end of described water delivery magnetic valve connects described microprocessor system, so as by described microprocessor system to implementation step 2) equipment plan as a whole control.
Step 2) in, described alcohol disinfecting adopts the alcohol flusher to implement, described alcohol flusher comprises an alcohol storage tank, at least one alcohol carrier pipe, at least one alcohol shower nozzle, described alcohol storage tank connects described shower nozzle by described alcohol carrier pipe, described shower nozzle is provided with a magnetic valve, described magnetic valve is that alcohol sprays valve, the control end that described alcohol sprays valve connects described microprocessor system, when described microprocessor system opens described alcohol and sprays valve, alcohol in the described alcohol holding vessel is delivered to described toasting by described alcohol carrier pipe with alcohol and sprays the valve place under positive pressure, and sprays alcohol.
Described alcohol shower nozzle is towards described shower nozzle setting, and described alcohol shower nozzle sprays alcohol to described shower nozzle, and bacterium residual on the shower nozzle is removed extremely.Described alcohol disinfecting both can carry out before described shower nozzle is washed away degerming by described sterile water, also can carry out after described shower nozzle is washed away degerming by described sterile water.Preferred described shower nozzle carries out described alcohol disinfecting before being washed away degerming by described sterile water.
In the step 3), described workbench comprises that one has the conveyer belt that transmits culture bottle, described conveyer belt be positioned at described shower nozzle under, the sterile water of ejection washes away described conveyer belt in the described shower nozzle, remove liquid spawn residual on the described conveyer belt and the bacterium that grows, the waste water on the described workbench adopts waste water collection device to collect.
Described waste water collection device comprises that one is used for the wastewater collection container of access waste water, one discharge of wastewater pipeline, described discharge of wastewater pipeline is communicated with described wastewater collection container, described wastewater collection container is positioned at the below of described workbench, waste water on the described workbench all drips in described wastewater collection container, and by described discharge of wastewater pipeline the waste water of collecting is transferred out.
Beneficial effect: after adopting above-mentioned technology, the present invention is simple in structure, and implementation cost is cheap, can effectively clean degerming to liquid-spawn inoculation equipment, lowers the pollution rate of liquid spawn, improves the survival rate of bacterial classification.
Description of drawings
Fig. 1 is a kind of operational flowchart of the present invention.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, further set forth the present invention below in conjunction with concrete diagram.
With reference to Fig. 1, reduce the inoculation method of operating of culture bottle pollution rate, comprise the steps:
1) bacterial classification sprays: the seeding tank that liquid spawn is housed is inflated, make the liquid spawn seeding tank of gushing out, liquid spawn is from exporting the shower nozzle that the liquid spawn conveyance conduit is connected to the tank connected liquid spawn conveyance conduit of seed, and shower nozzle is sprayed to liquid spawn in the culture bottle.
2) clean degerming: liquid spawn is injected sterile water to the liquid spawn conveyance conduit after spraying and finishing, and shower nozzle is washed degerming, and shower nozzle is carried out alcohol disinfecting.
3) wastewater treatment: the sterile water that sprays in the shower nozzle is sprayed to be used to the workbench of putting culture bottle, and the waste water after spraying is collected discharging.
After bacterial classification sprays, the solution of meeting residual liquid bacterial classification on the shower nozzle, solution contains abundant nutriment, easily by airborne bacteria adherence on shower nozzle, and carry out Fast-propagation by residual solution, liquid spawn to follow-up sprinkling is caused germ contamination, and the present invention is after each the sprinkling, utilize sterile water to carry out Flushing nozzle and liquid spawn conveyance conduit, both prevented that liquid spawn conveyance conduit and shower nozzle from stopping up, bacterium on the shower nozzle can be washed away cleaning again, reduce bacterium to the pollution of liquid spawn.
Embodiment 1:
As shown in Figure 1, during liquid-spawn inoculation, first the seeding tank that liquid spawn is housed is inflated, make the liquid spawn seeding tank of gushing out, liquid spawn is from exporting the shower nozzle that the liquid spawn conveyance conduit is connected to the tank connected liquid spawn conveyance conduit of seed, shower nozzle is sprayed to liquid spawn in the culture bottle, just can finish liquid-spawn inoculation one time.
Subsequently by microprocessor system closing bacterial classification magnetic valve, thereby disconnect seeding tank and liquid spawn conveyance conduit, first by the alcohol flusher shower nozzle is carried out alcohol disinfecting, utilize the characteristic of alcohol to kill the most of bacterium that is attached on the shower nozzle, then utilize water pump with in the sterile water filling liquid bacterial classification conveyance conduit, with the flow control of sterile water in the 0.5-1.5 metre per second (m/s), utilize the scouring force of sterile water that the residue on bacterial classification conveyance conduit and the shower nozzle is rinsed well, the bacterium that can will grow on shower nozzle is washed dilution, can reduce again alcohol to the impact of liquid spawn, effectively sterilizing.
Sterile water finish to the liquid spawn conveyance conduit wash away cleaning after, sterile water is at the driving-belt that sprays the zero load on the workbench under the shower nozzle effect, liquid spawn liquid residual on the conveyer belt is carried out flush clean, and the sewage after cleaning is dripping under Action of Gravity Field in the wastewater collection container, and transfers out by the discharge of wastewater pipeline.After the flushing of finishing the driving-belt of zero load, conveyer belt is transported to shower nozzle with culture bottle and issues, and the liquid spawn in the seeding tank is replenished complete, and under gas pressure, beginning the second liquid-spawn inoculation of taking turns.
Embodiment 2
In the liquid-spawn inoculation process, implement two liquid-spawn inoculations after, just the liquid spawn conveyance conduit is carried out a sterile water and washes away.
During the liquid spawn sowing, first seeding tank is inflated, gas atmosphere is controlled at 0.1Mpa, makes in the liquid spawn influent bacterial classification conveyance conduit in the seeding tank, and by shower nozzle liquid spawn is sprayed in the culture bottle.
Finish continuously above-mentioned steps after half an hour, by microprocessor system closing bacterial classification magnetic valve, thereby disconnect seeding tank and liquid spawn conveyance conduit, then utilize water pump that the liquid spawn carrier pipe is carried sterile water, and with the flow control of sterile water at the 1.5-3 metre per second (m/s), the effect of washing away to reach high pressure, the bacterium heap that is attached on the shower nozzle is carried out potent washing away, the sterile water that then will wash away from shower nozzle sprays the conveyer belt to zero load, residue on the conveyer belt is cleaned degerming, waste water after the cleaning degerming then drips in the wastewater collection container, collects discharging.At shower nozzle sterile water is all sprayed behind conveyer belt, the alcohol flusher sprays alcohol to shower nozzle, and shower nozzle is carried out two degree sterilization degerming, thoroughly removes the bacterium on the shower nozzle.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and the specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (10)
1. reduce the inoculation method of operating of culture bottle pollution rate, it is characterized in that, comprise the steps:
1) bacterial classification sprays: the seeding tank that liquid spawn is housed is inflated, make the liquid spawn seeding tank of gushing out, liquid spawn is from exporting the shower nozzle that the liquid spawn conveyance conduit is connected to the tank connected liquid spawn conveyance conduit of seed, and described shower nozzle is sprayed to liquid spawn in the culture bottle;
2) clean degerming: liquid spawn is injected sterile water to described liquid spawn conveyance conduit after spraying and finishing, and described shower nozzle is washed degerming, and described shower nozzle is carried out alcohol disinfecting;
3) wastewater treatment: the sterile water that sprays in the described shower nozzle is sprayed to be used to the workbench of putting culture bottle, and the waste water after spraying is collected discharging.
2. the inoculation method of operating of reduction culture bottle pollution rate according to claim 1, it is characterized in that: in described liquid-spawn inoculation process, implementation step 1 repeatedly) at least after half an hour, carry out one time step 2).
3. the inoculation method of operating of reduction culture bottle pollution rate according to claim 2 is characterized in that: the off time between twice step 1) in succession implementing is for implementing a step 2) operating time.
4. the inoculation method of operating of reduction culture bottle pollution rate according to claim 1, it is characterized in that: step 2) in, described liquid spawn conveyance conduit injects before the sterile water, first described seeding tank and liquid spawn conveyance conduit is disconnected, and stops described sterile water to flow in the described seeding tank.
5. the inoculation method of operating of reduction culture bottle pollution rate according to claim 4, it is characterized in that: be provided with a magnetic valve between described seeding tank and the described liquid spawn conveyance conduit, described magnetic valve is the bacterial classification magnetic valve, the control end of described bacterial classification magnetic valve connects a microprocessor system, by the described bacterial classification magnetic valve of opening and closing, realize described seeding tank and liquid spawn conveyance conduit connected sum are disconnected.
6. reduce according to claim 1 or 5 the inoculation method of operating of culture bottle pollution rate, it is characterized in that: step 2) in, the flow control of described sterile water is between 0.5 metre per second (m/s) to 3 metre per second (m/s).
7. the inoculation method of operating of reduction culture bottle pollution rate according to claim 1, it is characterized in that: carry the sterile water conveyance conduit of sterile water between described seeding tank and described shower nozzle, described sterile water conveyance conduit is communicated with described liquid spawn conveyance conduit, the junction of described sterile water conveyance conduit and described liquid spawn conveyance conduit is provided with another magnetic valve, described magnetic valve is the water delivery magnetic valve, and the control end of described water delivery magnetic valve connects described microprocessor system.
8. the inoculation method of operating of reduction culture bottle pollution rate according to claim 1, it is characterized in that: step 2) in, described alcohol disinfecting adopts the alcohol flusher to implement, described alcohol flusher comprises an alcohol storage tank, at least one alcohol carrier pipe, at least one alcohol shower nozzle, described alcohol storage tank connects described shower nozzle by described alcohol carrier pipe, described shower nozzle is provided with a magnetic valve, described magnetic valve is that alcohol sprays valve, and the control end that described alcohol sprays valve connects described microprocessor system;
When described microprocessor system opened described alcohol and sprays valve, the alcohol in the described alcohol holding vessel was delivered to described toasting by described alcohol carrier pipe with alcohol and sprays the valve place under positive pressure, and sprays alcohol.
9. the inoculation method of operating of reduction culture bottle pollution rate according to claim 8 is characterized in that: described alcohol shower nozzle is towards described shower nozzle setting, and described alcohol shower nozzle sprays alcohol to described shower nozzle, and bacterium residual on the shower nozzle is removed extremely;
Described alcohol disinfecting carried out before described shower nozzle is washed away degerming by described sterile water.
10. according to the inoculation method of operating of the reduction culture bottle pollution rate of claim described 1, it is characterized in that: in the step 3), described workbench comprises that one has the conveyer belt that transmits culture bottle, described conveyer belt be positioned at described shower nozzle under, the sterile water of ejection washes away described conveyer belt in the described shower nozzle, remove liquid spawn residual on the described conveyer belt and the bacterium that grows, the waste water on the described workbench adopts waste water collection device to collect;
Described waste water collection device comprises that one is used for the wastewater collection container of access waste water, one discharge of wastewater pipeline, described discharge of wastewater pipeline is communicated with described wastewater collection container, described wastewater collection container is positioned at the below of described workbench, waste water on the described workbench all drips in described wastewater collection container, and by described discharge of wastewater pipeline the waste water of collecting is transferred out.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012103166851A CN102972203A (en) | 2012-08-30 | 2012-08-30 | Inoculation operation method for reducing contamination rate of cultivating bottle |
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| CN2012103166851A CN102972203A (en) | 2012-08-30 | 2012-08-30 | Inoculation operation method for reducing contamination rate of cultivating bottle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535182A (en) * | 2013-08-14 | 2014-01-29 | 上海雪榕生物科技股份有限公司 | Intermittent disinfection method during operating process of liquid culture vaccination machine and vaccination machine with intermittent disinfection method used |
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| CN103535182B (en) * | 2013-08-14 | 2015-11-18 | 上海雪榕生物科技股份有限公司 | The inoculation device of the intermittent sterilization method in liquid strain inoculation machine running and employing the method |
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Application publication date: 20130320 |