CN112753557A - Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively - Google Patents

Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively Download PDF

Info

Publication number
CN112753557A
CN112753557A CN202110096090.9A CN202110096090A CN112753557A CN 112753557 A CN112753557 A CN 112753557A CN 202110096090 A CN202110096090 A CN 202110096090A CN 112753557 A CN112753557 A CN 112753557A
Authority
CN
China
Prior art keywords
gastrodia elata
seeds
culture
bacteria
filter paper
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110096090.9A
Other languages
Chinese (zh)
Inventor
高尚
曹小勇
秦公伟
袁洪超
赵垚
王斌
张娜
张孟琪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shaanxi Jinhuifang Traditional Chinese Medicine Technology Co ltd
Shaanxi University of Technology
Original Assignee
Shaanxi Jinhuifang Traditional Chinese Medicine Technology Co ltd
Shaanxi University of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shaanxi Jinhuifang Traditional Chinese Medicine Technology Co ltd, Shaanxi University of Technology filed Critical Shaanxi Jinhuifang Traditional Chinese Medicine Technology Co ltd
Priority to CN202110096090.9A priority Critical patent/CN112753557A/en
Publication of CN112753557A publication Critical patent/CN112753557A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A01G31/02—Special apparatus therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/40—Cultivation of spawn
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/60—Cultivation rooms; Equipment therefor
    • A01G18/64—Cultivation containers; Lids therefor
    • A01G18/68—Cultivation bottles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to the field of gastrodia elata cultivation, in particular to a device and a method capable of enabling gastrodia elata seeds to germinate uniformly and massively. Culturing liquid germinants: inoculating the germinating bacteria into a liquid culture medium of 1/2MS for culture, and after the germinating bacteria grow to a certain extent, fully shaking the germinating bacteria to break hyphae of the germinating bacteria; culturing gastrodia elata seeds: pouring the liquid germination bacteria into a culture bottle filled with sterile filter paper or sponge, after the germination bacteria grow over the whole filter paper, sprinkling the gastrodia elata seeds on the filter paper, and culturing the culture bottle at 25 ℃ in the dark for 20 days to obtain a large amount of gastrodia elata protocorms. The method is simple, and rhizoma Gastrodiae protocorm is easy to obtain.

Description

Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively
Technical Field
The invention relates to the field of gastrodia elata cultivation, in particular to a device and a method capable of enabling gastrodia elata seeds to germinate uniformly and massively.
Background
The gastrodia elata is a highly degenerated plant, has no root and green leaves, cannot absorb a large amount of moisture, inorganic and organic nutrients from soil, and cannot produce nutrients by photosynthesis through sunlight, the gastrodia elata seeds are fine, are capsules and have no endosperm, so that the gastrodia elata seeds are difficult to germinate in a natural state and a sterile culture medium, so that the nutrient sources of the seeds in the germination and vegetative growth stages are provided by fungi, but the germination of the gastrodia elata seeds is unstable, and the batch production technology of the gastrodia elata protocorms is not overcome. There are 5 kinds of germination bacteria symbiotic with gastrodia elata, namely osmundacola, orchiocola, dendrobe, dendrobii, anoectochilus, and Trametes lactinea Berkpat. The germination effect of each bacterium is different, and experiments prove that the dendrobe lentinus edodes is an ideal germinating bacterium. Armillaria mellea also has a plurality of strains, some strains can form good symbiotic relationship with the gastrodia elata, and some strains are not easy to form symbiotic relationship with the gastrodia elata. The life cycle of gastrodia elata includes the whole growth process from the sowing of seeds of the previous generation to the maturation of seeds of the next generation, i.e., from seeds → protocorm → rice hemp → white hemp → arrow hemp → seeds, which is called the first generation life history of gastrodia elata.
Disclosure of Invention
The purpose of the invention is as follows: in order to provide a device and a method which can ensure that the gastrodia elata seeds germinate uniformly and massively and have better effect, the specific purpose is seen in a plurality of substantial technical effects of the specific implementation part.
In order to achieve the purpose, the invention adopts the following technical scheme:
the first scheme is as follows:
a device capable of enabling gastrodia elata seeds to germinate uniformly and massively is characterized by comprising a first part 1 and a second part 4, wherein the first part 1 and the second part 4 can be connected through a threaded part 2, the second part 4 comprises a bottom 5, a sponge 6 is arranged in the second part 4, and the threaded part 2 is in threaded connection with a butt joint part I21 and a butt joint part II 22; the first part 1 and the second part 4 form a culture container and also comprise a spray head 9 which can be matched with the culture container, the spray head 9 is in a cone-like structure, the spray head 9 comprises a plurality of spray nozzles, the spray head 9 is connected with a pipeline 7, and the pipeline 7 is connected with a strain solution 13 and a hot water container 14 through a tee joint 12;
the pipe 7 comprises a pipe wrapping portion 18, the pipe wrapping portion 18 being wrapped around a rotating roller 17, the rotating roller 17 being located on the power shaft 16 of the power portion 15.
The further technical scheme of the invention is that the power part 15 is a positive and negative rotating motor.
A method for germinating rhizoma Gastrodiae uniformly and massively is characterized in that the device comprises the following steps,
and (3) preservation of gastrodia elata seeds: taking mature uncracked gastrodia elata fruits, cleaning the surfaces of the fruits, disinfecting the fruits for 30s by using 75% alcohol in an aseptic operation table, then treating the fruits for 15min by using 10% 84 disinfectant, cleaning the surfaces of the fruits by using sterile water, cutting the fruits by using a sterile scalpel in a sterile culture dish, and placing sterile seeds into an empty sterile culture bottle for storage;
preparation of sterile filter paper or sponge: placing filter paper in a culture flask, and sterilizing in a high temperature high pressure steam sterilizing pot for 20 min;
culturing liquid germinants: inoculating the germinating bacteria into a liquid culture medium of 1/2MS for culture, and after the germinating bacteria grow to a certain extent, fully shaking the germinating bacteria to break hyphae of the germinating bacteria;
culturing gastrodia elata seeds: pouring the liquid germination bacteria into a culture bottle filled with sterile filter paper or sponge, after the germination bacteria grow over the whole filter paper, sprinkling the gastrodia elata seeds on the filter paper, and culturing the culture bottle at 25 ℃ in the dark for 20 days to obtain a large amount of gastrodia elata protocorms.
The invention further adopts the technical scheme that the first part 1 and the second part 4 are combined, then hot water is downwards sprayed through the spray head for disinfection, and then the hot water is poured out; aseptic seed from shower nozzle and culture container's clearance on the downward sponge that falls, the shower nozzle is put by pipeline suitable downwards this moment, and the seed falls well back, and the shower nozzle is pulled up by the pipeline afterwards, realizes that shower nozzle outer wall and culture container's oral area agree with fixedly, realizes sealing.
Compared with the prior art, the invention adopting the technical scheme has the following beneficial effects: the method is simple, and rhizoma Gastrodiae protocorm is easy to obtain.
Drawings
To further illustrate the present invention, further description is provided below with reference to the accompanying drawings:
FIG. 1 is a schematic view of the inventive structure in perspective with the spout facing downward;
FIG. 2 is a schematic top perspective view of the inventive structure;
FIG. 3 is a piping connection diagram of the invention;
FIG. 4 is a butt-joint view of the first and second portions;
FIG. 5 is a schematic view of the overall structure of the middle transfer structure;
FIG. 6 is a perspective view of the inventive structure;
wherein: 1. a first portion; 2. a threaded portion; 3. rhizoma Gastrodiae protocorm; 4. a second portion; 5. a bottom; 6. a sponge; 7. a pipeline; 8. an upper opening; 9. a spray head; 10. an edge portion; 11. a middle transfer structure; 12. a tee joint; 13. a strain solution; 14. a hot water tank; 15. a power section; 16. a power shaft; 17. a rotating roller; 18. a pipe winding portion; 21. a first butt joint part; 22. and a second butting part.
Detailed Description
The present invention will be further illustrated with reference to the accompanying drawings and specific embodiments, which are to be understood as merely illustrative of the invention and not as limiting the scope of the invention. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The patent provides a plurality of parallel schemes, and different expressions belong to an improved scheme based on a basic scheme or a parallel scheme. Each solution has its own unique features.
The method is explained first:
the method for enabling the gastrodia elata seeds to germinate uniformly and massively can be used for enabling the gastrodia elata seeds to germinate uniformly, and the culture medium and the concentration of the culture medium which can be used in the method are wide, so long as the proper nutrition can be provided for the germination bacteria.
The method for enabling the gastrodia elata seeds to germinate uniformly and massively uses 75% alcohol for 30s and 10% 84% disinfectant for 15min to sterilize the gastrodia elata fruits, and the gastrodia elata fruits are washed with sterile water for 2-4 times.
The method for enabling the gastrodia elata seeds to germinate uniformly and in large quantity uses the filter paper as a carrier for growth of the germination bacteria and the gastrodia elata seeds, and the germination bacteria can be enabled to invade the seeds more easily.
The method for enabling the gastrodia elata seeds to germinate uniformly and massively uses a culture bottle made of glass or transparent plastic as a culture bottle, and can be used for observing the growth of hyphae and the germination process of the gastrodia elata seeds in detail.
The method for germinating the gastrodia elata seeds uniformly and massively has the advantages that the operation is simple, and the gastrodia elata protocorm is easy to obtain.
The method comprises the following specific implementation steps: 1, preservation of gastrodia elata seeds: taking mature uncracked rhizoma Gastrodiae fruit, cleaning the fruit surface, sterilizing with 75% alcohol for 30s in sterile operating platform, treating with 10% 84 disinfectant for 15min, cleaning with sterile water, cutting the fruit with sterile scalpel in sterile culture dish, and storing sterile seeds in empty sterile culture bottle.
Preparation of sterile filter paper: placing filter paper in culture flask, and sterilizing in high temperature high pressure steam sterilizing pot for 20 min.
Culturing liquid germinants: inoculating the germinating bacteria into a liquid culture medium of 1/2MS for culture, and after the germinating bacteria grow to a certain extent, fully shaking the germinating bacteria to break hyphae of the germinating bacteria.
Culturing gastrodia elata seeds: pouring the liquid germination bacteria into a culture bottle filled with sterile filter paper, after the germination bacteria grow over the filter paper, sprinkling the gastrodia elata seeds on the filter paper, and culturing the culture bottle at 25 ℃ in the dark for 20 days to obtain a large amount of gastrodia elata protocorms.
Example 1: during small-scale production, filter paper can be placed in a culture bottle, bacterial liquid is poured, seeds are scattered after germinating bacteria grow on the whole filter paper, and a large amount of gastrodia elata protocorms can be obtained after culturing for 20 days at 25 ℃ in a dark environment.
Example 2: during large-scale production, clean sponge can be placed on a culture shelf, a layer of filter paper or a screen is laid on the sponge, bacteria liquid is poured, after the germination bacteria grow, seeds are spread on the filter paper or the screen, and a large amount of gastrodia elata protocorms can be obtained after the gastrodia elata protocorms are cultured for 20 days at 25 ℃ in a dark environment.
The following description is made with reference to the apparatus:
combining the first part 1 and the second part 4, then spraying hot water downwards through the spray head, sterilizing, and then pouring out the hot water; aseptic seed from shower nozzle and culture container's clearance on the downward sponge that falls, the shower nozzle is put by pipeline suitable downwards this moment, and the seed falls well back, and the shower nozzle is pulled up by the pipeline afterwards, realizes that shower nozzle outer wall and culture container's oral area agree with fixedly, realizes sealing.
The spray head can conveniently spray water or hypha, and the spray head can also be matched with the mouth of the bottle after being pulled upwards.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are intended to illustrate the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and the invention is to be limited to the embodiments described above.

Claims (4)

1. A device capable of enabling gastrodia elata seeds to germinate uniformly and massively, and is characterized by comprising a first portion (1) and a second portion (4), wherein the first portion (1) and the second portion (4) can be connected through a threaded portion (2), the second portion (4) comprises a bottom (5), a sponge (6) is arranged in the second portion (4), and the threaded portion (2) is in threaded connection with a butt-joint portion I (21) and a butt-joint portion II (22); the first part (1) and the second part (4) form a culture container, and further comprise a spray head (9) which can be matched with the culture container, the spray head (9) is of a cone-like structure, the spray head (9) comprises a plurality of spray nozzles, the spray head (9) is connected with a pipeline (7), and the pipeline (7) is connected with a strain solution (13) and a hot water container (14) through a tee joint (12);
the pipeline (7) comprises a pipeline winding part (18), the pipeline winding part (18) is wound on a rotating roller (17), and the rotating roller (17) is positioned on a power shaft (16) of the power part (15).
2. The apparatus for uniform and mass germination of gastrodia elata seeds as claimed in claim 1, wherein the power part (15) is a counter-rotating motor.
3. A method for germinating rhizoma Gastrodiae uniformly and in large quantity, which comprises the following steps with the device of claim 1-2,
and (3) preservation of gastrodia elata seeds: taking mature uncracked gastrodia elata fruits, cleaning the surfaces of the fruits, disinfecting the fruits for 30s by using 75% alcohol in an aseptic operation table, then treating the fruits for 15min by using 10% 84 disinfectant, cleaning the surfaces of the fruits by using sterile water, cutting the fruits by using a sterile scalpel in a sterile culture dish, and placing sterile seeds into an empty sterile culture bottle for storage;
preparation of sterile filter paper or sponge: placing filter paper in a culture flask, and sterilizing in a high temperature high pressure steam sterilizing pot for 20 min;
culturing liquid germinants: inoculating the germinating bacteria into a liquid culture medium of 1/2MS for culture, and after the germinating bacteria grow to a certain extent, fully shaking the germinating bacteria to break hyphae of the germinating bacteria;
culturing gastrodia elata seeds: pouring the liquid germination bacteria into a culture bottle filled with sterile filter paper or sponge, after the germination bacteria grow over the whole filter paper, sprinkling the gastrodia elata seeds on the filter paper, and culturing the culture bottle at 25 ℃ in the dark for 20 days to obtain a large amount of gastrodia elata protocorms.
4. A method for uniform and mass germination of seeds of gastrodia elata as claimed in claim 3, wherein the first part (1) and the second part (4) are combined and then sterilized by spraying hot water downwardly through a nozzle, and then pouring out the hot water; aseptic seed from shower nozzle and culture container's clearance on the downward sponge that falls, the shower nozzle is put by pipeline suitable downwards this moment, and the seed falls well back, and the shower nozzle is pulled up by the pipeline afterwards, realizes that shower nozzle outer wall and culture container's oral area agree with fixedly, realizes sealing.
CN202110096090.9A 2021-01-25 2021-01-25 Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively Pending CN112753557A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110096090.9A CN112753557A (en) 2021-01-25 2021-01-25 Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110096090.9A CN112753557A (en) 2021-01-25 2021-01-25 Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively

Publications (1)

Publication Number Publication Date
CN112753557A true CN112753557A (en) 2021-05-07

Family

ID=75707069

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110096090.9A Pending CN112753557A (en) 2021-01-25 2021-01-25 Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively

Country Status (1)

Country Link
CN (1) CN112753557A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568064A (en) * 2022-03-01 2022-06-03 广西南亚热带农业科学研究所 Pineapple seed breathable water-retention breeding method
CN117223542A (en) * 2023-11-15 2023-12-15 山东金必来生物科技有限公司 Arbuscular mycorrhizal fungus mycelium collector and collection method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568064A (en) * 2022-03-01 2022-06-03 广西南亚热带农业科学研究所 Pineapple seed breathable water-retention breeding method
CN117223542A (en) * 2023-11-15 2023-12-15 山东金必来生物科技有限公司 Arbuscular mycorrhizal fungus mycelium collector and collection method
CN117223542B (en) * 2023-11-15 2024-02-23 山东金必来生物科技有限公司 Arbuscular mycorrhizal fungus mycelium collector and collection method

Similar Documents

Publication Publication Date Title
CN105145352B (en) A kind of bletilla seed height of seedling imitates group culturation rapid propagating technology
CN101258835B (en) Fast reproducing method for high quality seedling of dendrobium officinale
CN102668878B (en) Method for cultivating flammulina velutipes by using aquilaria-sinensis-chip edible mushroom culture medium
CN103004469B (en) Tall gastrodia tuber culture method, tall gastrodia tuber liquor and tall gastrodia tuber production process by using small-caliber container
CN101773052A (en) Preparation and industrial culture of aweto strain
CN105638467B (en) A kind of method that roxburgh anoectochilus terminal bud is cultivated with culture bag
CN112753557A (en) Device and method capable of enabling gastrodia elata seeds to germinate uniformly and massively
CN105638477A (en) Rapid propagation method for dendrobium hancockii seeds
CN101116424B (en) Highly effective lily bulblet inducement culture method
CN105993865A (en) Cultivation method for quercus variabilis aseptic seedling
CN105961197A (en) High efficiency regeneration method of Moringa oleifera
CN105191803B (en) A kind of candidum tissue culturing bag seedling production method
CN101695280B (en) Tissue culture and rapid propagation method of raspberries
CN106900555A (en) Amomum viosum test tube plant division culture medium and once-seedling forming tissue culture plant division quick-breeding method
CN111919749B (en) Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof
CN117546744A (en) Planting method of gastrodia elata
CN101622922B (en) Symbiotic germination method of Chinese cymbidium hybrid seeds and bacteria
CN105309301B (en) Dendrobium Chrysotoxum Lindl seedling commercial production method
CN214546322U (en) Device capable of enabling gastrodia elata seeds to germinate uniformly and in large quantity
CN105820970A (en) Bacillus megaterium strain and application thereof
CN104813940B (en) A kind of quick breeding method for tissue culture of Chinese asystasiella herb
CN107581068B (en) A kind of method removing single-shaft orchid endophyte and the application in vanilla tissue-culturing quick-propagation
CN105746350A (en) Rapid propagation method for corm tissue culture of Nervilia fordii
CN113598046B (en) Efficient tissue culture breeding method for ficus microcarpa
CN104026011B (en) The substratum of the tissue-culturing quick-propagation of silver leaf climbing fig and propagation method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination