CN102961998B - Rotary shaker for microorganism and molecular biological experiments - Google Patents
Rotary shaker for microorganism and molecular biological experiments Download PDFInfo
- Publication number
- CN102961998B CN102961998B CN201210484067.8A CN201210484067A CN102961998B CN 102961998 B CN102961998 B CN 102961998B CN 201210484067 A CN201210484067 A CN 201210484067A CN 102961998 B CN102961998 B CN 102961998B
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- case
- rotary
- retort
- air
- ring cavity
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Abstract
The invention discloses a rotary shaker for microorganism and molecular biological experiments. The rotary shaker is characterized by comprising an L-type constant temperature tank body, a reaction pot, a pair of support seats, and a drive device for driving the reaction pot to rotate, wherein two ends of the reaction pot are connected with ring cavities; the pair of support seats supports the reaction pot; the L-type tank body is divided into two parts; a vertical tank body at the rear part is a cold air box; a horizontal tank body at the front part is a hot air box; a cold (hot) air outlet with a valve is arranged in one side of the cold (hot) air box, and connected with a tee joint through an air duct outside the box; the support seats are arranged on the hot air box, one support seat is fixed and the other support seat is relatively moved and located; the support seats are respectively connected with a driving rotary terminal and a driven rotary terminal through bearings; the two rotary terminals are of structures in which the middle parts are thick, two ends are thin, and the centers are hollow; the opposite ends of the two rotary terminals are respectively inserted into the ring cavities connected to two ends of the reaction pot to be coincided and sealed; and one of the reversed ends of the two rotary terminals is a constant-temperature air inlet, and the constant-temperature air inlet is connected with a third outlet of the tee joint through the air duct.
Description
Technical field
The present invention relates to a kind of device for clinical medicine and Basic medicine experiment, particularly in a kind of amplification of the bacterium for Experiment on Microbiology, molecular biosciences experiment, make reactant be in all the time under flow regime and well-bound multi-function device.
Background technology
In recent years, along with the development and progress of medical domain, molecular biology and the application of Microbiology Experiment technology in research work are more extensive, the secret that researcher is explored life science has had abundanter means, innovative idea is more and more many, experimental technique is ripe day by day, and required instrument kind is tending towards variation.For example, in molecular biology experiment (such as westernblot and co-immunoprecipitation etc.) and Experiment on Microbiology (plasmid extraction), in order to impel bacterium amplification, make between reactant, there is more sufficient combination, experimenter often need to utilize a kind of special device " shaking table ", makes reaction system be in all the time flow regime.The shaking table sold in the market comprises two kinds of dull and stereotyped rotary and Spring Pendulum dynamic formulas, and they do not possess under constant temperature, cryogenic conditions and use.Therefore have to shaking table to be placed in refrigerator (refrigerator), this has taken limited space resources in case, cabinet on the one hand, affect constant temperature effect because shaking table external cord easily causes cabinet door to be not closed completely on the other hand, can not meet the needs of vast researcher to some special experiment.
Summary of the invention
For the existing problem of existing shaking table, the object of this invention is to provide a kind of economical and practical type rotary shaker can high low temperature dual-purpose, constant temperature variable-ratio.
For reaching above object, the present invention takes following technical scheme to be achieved:
A kind of rotary shaker for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there is the transmission device that connects the retort of ring cavity, a pair of supporting base that supports this retort, the rotation of driving retort at " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is hot gas case, is provided with refrigerating plant in cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; In hot gas case, be provided with heating combined equipment, hot gas case one side is provided with a heat outlet with valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above hot gas case, and one of them is fixed, and another is moved and locate heating the relatively-stationary supporting base in case surface by slide rail, on fixed mounting base, connects active rotation terminal by bearing; On another relative supporting base, connect passive rotary terminal by bearing, active and passive rotary terminal is thin ends and thick middle structure, center is hollow, and peg graft respectively retort two ends and being connected in ring cavity of being communicated with in tank in two rotary terminals one end in opposite directions, and the sealing that coincide; One end that two rotary terminals are opposing, one is constant temperature gas feed, and another is constant temperature gas vent, and wherein, constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.
In such scheme, described constant temperature gas vent is directly communicated with airborne release, or is recycled in hot and cold case by the pipeline mode identical with gas handling system, recycles.
Described transmission device comprises, active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near variable speed electric motors, particularly fixed mounting base on case and is connected with one by driving-belt.
Described retort by insulation material make can folding two and half cylindrical shells, half center, cylindrical shell two ends is provided with half ring cavity, one of the half ring cavity composition at two ends and the ring cavity that is connected of connection in tank when two and half cylindrical shells are tightly connected relatively.
Two and half cylindrical shells of described retort, axially arrange an air-tight bottle on one of them half cylindrical shell inner tube wall, on another half cylindrical shell inner tube wall, multiple test tubes are axially set, and air-tight bottle or test tube are connected with inner tank wall by deck.
It is a notched ring-type that described deck is made cross section by elastomeric material, relatively at the bottom of the ring of breach, is fixed in inner tank wall, and this breach spacing is less than the diameter of air-tight bottle or test tube.
Before described hot gas case, leave louvre and airstrainer for gas exchange.
Advantage of the present invention is, multi-functional, can under high (low) temperature, constant temperature, Variable Velocity Condition, use simultaneously, and good constant-temperature effect, simple to operate, meet the needs of researcher to some special experiment.
Brief description of the drawings
Fig. 1 is rotary shaker overall structure schematic diagram of the present invention.
Fig. 2 is the structural representation of retort in Fig. 1.
Fig. 3 be in Fig. 2 A-A to partial sectional view.
In Fig. 1 to Fig. 3: 1, cold air case; 2, cold air outlet; 3, constant temperature gas feed; 4, heat outlet; 5, driving-belt; 6, hot gas case; 7, variable speed electric motors, particularly; 8, retort; 9, heater; 10, supporting base; 11, constant temperature gas vent; 12, passive rotary terminal; 13, refrigerating plant; 14, active rotation terminal; 15, threeway; 16, half ring cavity; 17, air-tight bottle; 18, deck; 19, half cylindrical shell; 20, test tube; 21.Inner tank wall.
Detailed description of the invention
With reference to figure 1, for a rotary shaker for microorganism and molecular biosciences experiment, comprise " L " type thermostatic box, one by insulation material make can folding retort 8, support this retort a pair of supporting base 10, drive the cold heat outlet of transmission device, thermostatic box of retort rotation and connect the pipeline (not shown in FIG.) of cold heat outlet.
" L " molding box body is divided into two parts, the upright casing in rear portion is cold air case 1, the casing of anterior horizontal is hot gas case 6, in cold air case 1, be provided with refrigerating plant 13(compressor, evaporation and condenser pipe, temperature sensor etc.), cold air case one side is provided with cold air outlet 2, valve (not shown in FIG.) can be set on it, be connected with a threeway 15 by the outer wireway of case.Heating combined equipment 9(heater element, fan, temperature sensor etc. are housed) in hot gas case 6, leave louvre and airstrainer (not shown in FIG.) for gas exchange above, hot gas case one side (with cold air outlet 2 homonymies) has a heat outlet 4, on it, valve can be set, by the outer wireway of case with threeway 15 be connected.
The a pair of supporting base 10 that supports retort 8 is arranged on above hot gas case 6, one of them is fixed on hot gas case, another supporting base, by slide rail (not shown in FIG.), can move and locate relative to fixed mounting base along directions X on hot gas case surface, to facilitate installation, dismounting retort 8.On fixed mounting base, connect active rotation terminal 14 by bearing; On another relative supporting base, connect passive rotary terminal by bearing.Active and passive rotary terminal 14,12 is thin ends and thick middle structure, and center is hollow, and peg graft (with being communicated with in tank) in the connection ring cavity at retort 8 two ends in two rotary terminals one end in opposite directions, and the sealing (with reference to figure 2) that coincide; The opposing one end of two rotary terminals is respectively constant temperature gas feed 3, constant temperature gas vent 11, and wherein, constant temperature gas feed 3 is connected with the 3rd mouth of threeway 15 by wireway.Constant temperature gas vent 11 is directly communicated with airborne release, also can be recycled in hot and cold case by the mode identical with gas handling system (hot and cold gas import and valve are set on cold and hot gas tank, also connect constant temperature gas vent 11 by another threeway and conduit), recycle.
Active rotation terminal 14 be provided with annular groove by driving-belt 5(as belt) be connected with an output wheel that is arranged near the variable speed electric motors, particularly 7 of (fixed mounting base) on hot gas case.Variable speed electric motors, particularly 7 can regulate its velocity of rotation by speed-regulating switch, indirectly to control retort 8 rotary speeies.
With reference to figure 2, retort 8 is relatively tightly connected and is formed (removable) by two and half cylindrical shells 19, and when 16, two half cylindrical shells of half ring cavity that half center, cylindrical shell two ends arranges are tightly connected relatively, half ring cavity 16 at two ends forms one and connects ring cavity (with being communicated with in tank).On one of them half cylindrical shell inner tube wall, an air-tight bottle 17 is axially set, on another half cylindrical shell inner tube wall, multiple test tubes 20 is axially set.Air-tight bottle 17, test tube 20 balance mutually, reduces retort rotational resistance, increases the service life.Air-tight bottle 17, test tube 20 can be connected with inner tank wall 21 by deck 18 is convenient.
With reference to figure 3, deck 18 is made (as hard plastic rubber) by elastomeric material, its cross section is a notched ring-type, relatively at the bottom of the ring of breach, be fixed in inner tank wall 21, breach size should be less than the diameter of air-tight bottle or test tube, in the time that air-tight bottle or test tube have just embedded deck from indentation, there, this breach pressurized opens; After air-tight bottle or test tube enter in ring-type deck, indentation, there retraction can not be deviate from air-tight bottle or test tube.
The operation principle of rotary shaker of the present invention is, in 8 two half cylindrical shells of retort, install reaction bulb and test tube, two and half cylindrical shells 19 are tightly connected relatively, make a supporting base move and locate relative to fixed mounting base along directions X on hot gas case surface by slide rail, pegged graft in the connection ring cavity at retort 8 two ends in two rotary terminals one end in opposite directions; Need to select to open cold (heat) gas outlet valve according to hot and cold experiment, cold (heat) gas in cold (heat) gas tank is poured in retort by threeway and constant temperature gas feed 3, make reaction bulb and test tube in tank maintain constant temperature; Start variable speed electric motors, particularly 7, make retort by certain speed rotation, under constant temperature, make reaction system be in all the time flow regime, between reactant, have more sufficient combination.Cold air case can be the constant temperature cold air that provides 4 DEG C in retort 8, carries out organizing in immune precipitation pvdf membrane and primary antibodie association reaction in lysate and antibody association reaction or western blot experiment.Hot gas case can be in retort 8 37 DEG C of constant temperature hot gas is provided, and carries out the sealing of pvdf membrane in plasmid amplified reaction in bacterium in molecular cloning or western blot test.And the rotating speed of retort 8 can be controlled to per minute 50 ~ 100 by variable speed electric motors, particularly 7 and turn, to meet the needs of different experiments.
Claims (3)
1. the rotary shaker for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there is the transmission device that connects the retort of ring cavity, a pair of supporting base that supports this retort, the rotation of driving retort at " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is hot gas case, is provided with refrigerating plant in cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; In hot gas case, be provided with heating combined equipment, hot gas case one side is provided with a heat outlet with valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above hot gas case, and one of them is fixed, and another is moved and locate heating the relatively-stationary supporting base in case surface by slide rail, on fixed mounting base, connects active rotation terminal by bearing; On another relative supporting base, connect passive rotary terminal by bearing, active and passive rotary terminal is thin ends and thick middle structure, center is hollow, and the sealing that coincide in the connection ring cavity at retort two ends of pegging graft respectively of two rotary terminals one end in opposite directions, connects ring cavity and be communicated with tank is interior; One end that two rotary terminals are opposing, one is constant temperature gas feed, and another is constant temperature gas vent, and wherein, constant temperature gas feed is connected with the 3rd mouth of threeway by wireway;
Described retort by insulation material make can folding two and half cylindrical shells, half center, cylindrical shell two ends is provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively, the ring cavity that is connected of one of the half ring cavity composition at two ends and connection in tank;
Two and half cylindrical shells of described retort, axially arrange an air-tight bottle on one of them half cylindrical shell inner tube wall, on another half cylindrical shell inner tube wall, multiple test tubes are axially set, and air-tight bottle or test tube are connected with inner tank wall by deck.
2. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1, it is characterized in that, described transmission device comprises, active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near variable speed electric motors, particularly fixed mounting base on case and is connected with one by driving-belt.
3. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1, it is characterized in that, it is a notched ring-type that described deck is made cross section by elastomeric material, relatively at the bottom of the ring of breach, is fixed in inner tank wall, and this breach spacing is less than the diameter of air-tight bottle or test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210484067.8A CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210484067.8A CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102961998A CN102961998A (en) | 2013-03-13 |
| CN102961998B true CN102961998B (en) | 2014-10-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210484067.8A Expired - Fee Related CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
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| CN (1) | CN102961998B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141746A (en) * | 2019-06-18 | 2019-08-20 | 河南省中医院（河南中医药大学第二附属医院） | Breathe internal medicine with helping inhaling ware |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87211803U (en) * | 1987-08-10 | 1988-09-28 | 任克宁 | Speed-adjustable rotary culture device |
| RU2150097C1 (en) * | 1997-03-17 | 2000-05-27 | Извозчиков Илья Борисович | Gear to treat and impregnate histological samples |
| WO2000060362A1 (en) * | 1999-04-08 | 2000-10-12 | Chung Chang Young | Apparatus for fast preparation and analysis of nucleic acids |
| CN1131303C (en) * | 1999-11-16 | 2003-12-17 | 中国科学院力学研究所 | Three-dimensional discal rotary hollow on-line cell cultivator with air and liquid supply |
| CN201676659U (en) * | 2010-05-24 | 2010-12-22 | 杭州奥盛仪器有限公司 | Constant temperature blending instrument |
| CN203002284U (en) * | 2012-11-23 | 2013-06-19 | 西安医学院第二附属医院 | Rotary table for experiments on micro-organisms and molecular organisms |
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2012
- 2012-11-23 CN CN201210484067.8A patent/CN102961998B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN102961998A (en) | 2013-03-13 |
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Granted publication date: 20141022 Termination date: 20161123 |