CN203002284U - Rotary table for experiments on micro-organisms and molecular organisms - Google Patents

Rotary table for experiments on micro-organisms and molecular organisms Download PDF

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Publication number
CN203002284U
CN203002284U CN 201220631244 CN201220631244U CN203002284U CN 203002284 U CN203002284 U CN 203002284U CN 201220631244 CN201220631244 CN 201220631244 CN 201220631244 U CN201220631244 U CN 201220631244U CN 203002284 U CN203002284 U CN 203002284U
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CN
China
Prior art keywords
case
gas
retort
box
experiment
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Withdrawn - After Issue
Application number
CN 201220631244
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Chinese (zh)
Inventor
赵海康
赵鸽
王建榜
张学策
孙小龙
方伟
张研春
颜林枫
南海燕
夏毅
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Second Affiliated Hospital of Xian Medical University
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Second Affiliated Hospital of Xian Medical University
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Priority to CN 201220631244 priority Critical patent/CN203002284U/en
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Publication of CN203002284U publication Critical patent/CN203002284U/en
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Abstract

The utility model discloses a rotary table for experiments on micro-organisms and molecular organisms. The rotary table is characterized by comprising an L-shaped thermostat box, a reaction tank with connecting ring cavities at both ends, a pair of bearing seats for supporting the reaction tank, and a drive device for driving the reaction tank to rotate, wherein the L-shaped thermostat box is divided into two parts, the rear vertical box body is a cool-gas box, the front horizontal box body is a hot-gas box, and a cool-gas (hot-gas) outlet with a valve is formed in one side of the cool-gas (hot-gas) box, and connected with a three-way via a gas guide tube outside the box; the bearing seats are arranged on the hot-gas box, one of the bearing seats is fixed, the other one is moved and located relative to the fixed bearing seat, and an active rotating terminal and a passive rotating terminal are connected to the bearing seats via bearings respectively; the two rotating terminals are of a structure with a thick middle and two thin ends respectively, the centres of the rotating terminals are hollow, and the facing ends of the two rotating terminals are inserted in the connecting ring cavities at the both ends of the reaction tank respectively and then are fitted and sealed; and the departing ends of the two rotating terminals are constant-temperature gas inlets, and connected with the third port of the three-way via gas guide tubes.

Description

A kind of rotary shaker for microorganism and molecular biosciences experiment
Technical field
The utility model relates to a kind of device for clinical medicine and Basic medicine experiment, particularly makes reactant be in all the time under flow regime and well-bound multi-function device in a kind of bacterium amplification for Experiment on Microbiology, molecular biosciences experiment.
Background technology
In recent years, development and progress along with medical domain, molecular biology and the application of Microbiology Experiment technology in research work are more extensive, the secret that researcher is explored life science has had more abundant means, innovative idea is more and more many, experimental technique is ripe day by day, and required instrument kind is tending towards variation.In molecular biology experiment (such as westernblot and co-immunoprecipitation etc.) and Experiment on Microbiology (for example plasmid extraction), in order to impel bacterium amplification, to make more sufficient combination is arranged between reactant, the experimenter often need to utilize a kind of device " shaking table " of special use, makes reaction system be in all the time flow regime.The shaking table of selling in the market comprises two kinds of dull and stereotyped rotary and Spring Pendulum dynamic formulas, and they do not possess under constant temperature, cryogenic conditions uses.Therefore have to shaking table is placed in refrigerator (refrigerator), this has taken limited space resources in case, cabinet on the one hand, affect the constant temperature effect because the shaking table external cord easily causes cabinet door to be not closed completely on the other hand, can not satisfy vast researcher to the needs of some special experiment.
Summary of the invention
For the existing problem of existing shaking table, the purpose of this utility model is to provide a kind of can high low temperature dual-purpose, the economical and practical type rotary shaker constant temperature variable-ratio.
For reaching above purpose, the utility model takes following technical scheme to be achieved:
A kind of rotary shaker for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there are retort, a pair of supporting base that supports this retort that connects ring cavity, the transmission device that drives the retort rotation in " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is the hot gas case, is provided with refrigerating plant in cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; Be provided with heating combined equipment in the hot gas case, hot gas case one side is provided with one with the heat outlet of valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above the hot gas case, and one of them is fixed, and another moves and locate heating the relatively-stationary supporting base in case surface by slide rail, connects the active rotation terminal by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base, main, passive rotary terminal is thin ends and thick middle structure, the center is hollow, and what two a rotary terminals end was in opposite directions pegged graft respectively and is communicated with in retort two ends and tank is connected in ring cavity, and the sealing that coincide; The end that two rotary terminals are opposing, one is the constant temperature gas feed, and another is the constant temperature gas vent, and wherein, the constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.
In such scheme, described constant temperature gas vent directly is communicated with airborne release, or is recycled in hot and cold case by the pipeline mode identical with gas handling system, carries out recycling.
Described transmission device comprises, the active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near the variable speed electric motors, particularly fixed mounting base on case and is connected with one by driving-belt.
But described retort is made two and half cylindrical shells of folding by insulation material, and half center, cylindrical shell two ends is provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively half ring cavity at two ends form one with tank in connection be connected ring cavity.
Two and half cylindrical shells of described retort axially arrange an air-tight bottle on one of them half cylindrical shell inner tube wall, on another half cylindrical shell inner tube wall, a plurality of test tubes are set axially, and air-tight bottle or test tube are connected with inner tank wall by deck.
It is a notched ring-type that described deck is made the cross section by elastomeric material, relatively is fixed on inner tank wall at the bottom of the ring of breach, and this breach spacing is less than the diameter of air-tight bottle or test tube.
Louvre and the airstrainer for the gas exchange left in described hot gas case front.
The utility model has the advantages that, multi-functional, can use under high (low) temperature, constant temperature, Variable Velocity Condition simultaneously, and good constant-temperature effect, simple to operate, satisfy researcher to the needs of some special experiment.
Description of drawings
Fig. 1 is the utility model rotary shaker overall structure schematic diagram.
Fig. 2 is half structural representation when in Fig. 1, retort is separated.
Fig. 3 is second half structural representation when in Fig. 1, retort is separated.
Fig. 4 be in Fig. 2 or Fig. 3 A-A to partial sectional view.
In Fig. 1 to Fig. 4: 1, cold air case; 2, cold air outlet; 3, constant temperature gas feed; 4, heat outlet; 5, driving-belt; 6, hot gas case; 7, variable speed electric motors, particularly; 8, retort; 9, heater; 10, supporting base; 11, constant temperature gas vent; 12, passive rotary terminal; 13, refrigerating plant; 14, active rotation terminal; 15, threeway; 16, half ring cavity; 17, air-tight bottle; 18, deck; 19, half cylindrical shell; 20, test tube; 21.Inner tank wall.
The specific embodiment
With reference to figure 1, a kind of rotary shaker for the experiment of microorganism and molecular biosciences, comprise " L " type thermostatic box, one by insulation material make can folding retort 8, a pair of supporting base 10 that supports this retort, the cold and hot gas outlet of transmission device, thermostatic box that drives the retort rotation and the pipeline (not shown in FIG.) that connects cold and hot gas outlet.
" L " molding box body is divided into two parts, the upright casing in rear portion is cold air case 1, the casing of anterior horizontal is hot gas case 6, be provided with refrigerating plant 13(compressor, evaporation and condenser pipe, temperature sensor etc. in cold air case 1), cold air case one side is provided with cold air outlet 2, valve (not shown in FIG.) can be set on it, be connected with a threeway 15 by the outer wireway of case.Heating combined equipment 9(heater element, fan, temperature sensor etc. are housed) in hot gas case 6, louvre and the airstrainer (not shown in FIG.) for the gas exchange left in the front, hot gas case one side (with cold air outlet 2 homonymies) has a heat outlet 4, on it, valve can be set, by the outer wireway of case with threeway 15 be connected.
The a pair of supporting base 10 that supports retort 8 is arranged on above hot gas case 6, one of them is fixed on the hot gas case, another supporting base can move and locate relative to fixed mounting base along directions X on hot gas case surface by slide rail (not shown in FIG.), with convenient installation, dismounting retort 8.Connect active rotation terminal 14 by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base.Main, passive rotary terminal 14,12 is thin ends and thick middle structure, and the center is hollow, and two a rotary terminals end is in opposite directions pegged graft (with being communicated with in tank) in the connection ring cavity at retort 8 two ends, and the sealing (with reference to figure 2, Fig. 3) that coincide; The opposing end of two rotary terminals is respectively constant temperature gas feed 3, constant temperature gas vent 11, and wherein, constant temperature gas feed 3 is connected with the 3rd mouth of threeway 15 by wireway.Constant temperature gas vent 11 directly is communicated with airborne release, also can be recycled in hot and cold case by the mode identical with gas handling system (hot and cold gas import and valve are set on cold and hot gas tank, also connect constant temperature gas vent 11 by another threeway and conduit), carry out recycling.
Active rotation terminal 14 is provided with annular groove by driving-belt 5(such as belt) be connected with an output wheel that is arranged near the variable speed electric motors, particularly 7 of (fixed mounting base) on the hot gas case.Variable speed electric motors, particularly 7 can be regulated its velocity of rotation by speed-regulating switch, with indirectly control retort 8 rotary speeies.
With reference to figure 2, Fig. 3, retort 8 relatively is tightly connected by two and half cylindrical shells 19 and forms (removable), when 16, two half cylindrical shells of half ring cavity that half center, cylindrical shell two ends arrange are tightly connected relatively, half ring cavity 16 at two ends forms one and connects ring cavity (with being communicated with in tank).An air-tight bottle 17(Fig. 2 axially is set on one of them half cylindrical shell inner tube wall), a plurality of test tube 20(Fig. 3 axially are set) on another half cylindrical shell inner tube wall.Air-tight bottle 17, test tube 20 be balance mutually, reduces the retort rotational resistance, increases the service life.Air-tight bottle 17, test tube 20 can be connected with inner tank wall 21 by deck 18 is convenient.
With reference to figure 4, deck 18 is made (as hard plastic rubber) by elastomeric material, its cross section is a notched ring-type, relatively be fixed on inner tank wall 21 at the bottom of the ring of breach, the breach size should be less than the diameter of air-tight bottle or test tube, when air-tight bottle or test tube had just embedded deck from indentation, there, this breach pressurized opened; After air-tight bottle or test tube entered in the ring-type deck, the indentation, there retraction can not deviate from air-tight bottle or test tube.
The operation principle of the utility model rotary shaker is, install reaction bulb and test tube in 8 two half cylindrical shells of retort, two and half cylindrical shells 19 are tightly connected relatively, make a supporting base move and locate relative to fixed mounting base along directions X on hot gas case surface by slide rail, two a rotary terminals end is in opposite directions pegged graft in the connection ring cavity at retort 8 two ends; Select to open cold (heat) gas outlet valve according to hot and cold experiment needs, cold (heat) gas in cold (heat) gas tank is poured in retort by threeway and constant temperature gas feed 3, make in tank reaction bulb and test tube keep constant temperature; Start variable speed electric motors, particularly 7, make retort by certain speed rotation, make reaction system be in all the time flow regime under constant temperature, more sufficient combination is arranged between reactant.Cold air case can be provides the constant temperature cold air of 4 ℃ in retort 8, carry out organizing in immune precipitation pvdf membrane and primary antibodie association reaction in lysate and antibody association reaction or western blot experiment.The hot gas case can be provides 37 ℃ of constant temperature hot gas in retort 8, carry out the sealing of pvdf membrane in plasmid amplified reaction in bacterium in molecular cloning or western blot test.And the rotating speed of retort 8 can be controlled at per minute 50~100 by variable speed electric motors, particularly 7 and turn, to satisfy the needs of different experiments.

Claims (7)

1. rotary shaker that is used for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there are retort, a pair of supporting base that supports this retort that connects ring cavity, the transmission device that drives the retort rotation in " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is the hot gas case, is provided with refrigerating plant in cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; Be provided with heating combined equipment in the hot gas case, hot gas case one side is provided with one with the heat outlet of valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above the hot gas case, and one of them is fixed, and another moves and locate heating the relatively-stationary supporting base in case surface by slide rail, connects the active rotation terminal by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base, main, passive rotary terminal is thin ends and thick middle structure, the center is hollow, and the sealing that coincide in the connection ring cavity at retort two ends of pegging graft respectively of two a rotary terminals end in opposite directions connects ring cavity and is communicated with tank is interior; The end that two rotary terminals are opposing, one is the constant temperature gas feed, and another is the constant temperature gas vent, and wherein, the constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.
2. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1, is characterized in that, described constant temperature gas vent directly is communicated with atmosphere, or is recycled in hot and cold case by the pipeline mode identical with gas handling system, carries out recycling.
3. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, described transmission device comprises, the active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near the variable speed electric motors, particularly fixed mounting base on case and is connected with one by driving-belt.
4. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, but described retort is made two and half cylindrical shells of folding by insulation material, half center, cylindrical shell two ends are provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively, half ring cavity at two ends form one with tank in connection be connected ring cavity.
5. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 4, it is characterized in that, two and half cylindrical shells of described retort, on one of them half cylindrical shell inner tube wall, an air-tight bottle is set axially, on another half cylindrical shell inner tube wall, a plurality of test tubes are set axially, air-tight bottle or test tube are connected with inner tank wall by deck.
6. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 5, it is characterized in that, it is a notched ring-type that described deck is made the cross section by elastomeric material, relatively is fixed on inner tank wall at the bottom of the ring of breach, and this breach spacing is less than the diameter of air-tight bottle or test tube.
7. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, is characterized in that, louvre and the airstrainer for the gas exchange left in described hot gas case front.
CN 201220631244 2012-11-23 2012-11-23 Rotary table for experiments on micro-organisms and molecular organisms Withdrawn - After Issue CN203002284U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220631244 CN203002284U (en) 2012-11-23 2012-11-23 Rotary table for experiments on micro-organisms and molecular organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220631244 CN203002284U (en) 2012-11-23 2012-11-23 Rotary table for experiments on micro-organisms and molecular organisms

Publications (1)

Publication Number Publication Date
CN203002284U true CN203002284U (en) 2013-06-19

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CN 201220631244 Withdrawn - After Issue CN203002284U (en) 2012-11-23 2012-11-23 Rotary table for experiments on micro-organisms and molecular organisms

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102961998A (en) * 2012-11-23 2013-03-13 西安医学院第二附属医院 Rotary shaker for microorganism and molecular biological experiments

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102961998A (en) * 2012-11-23 2013-03-13 西安医学院第二附属医院 Rotary shaker for microorganism and molecular biological experiments

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AV01 Patent right actively abandoned

Granted publication date: 20130619

Effective date of abandoning: 20141022

AV01 Patent right actively abandoned

Granted publication date: 20130619

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