CN102961998A - Rotary shaker for microorganism and molecular biological experiments - Google Patents

Abstract

The invention discloses a rotary shaker for microbiology and molecular biology experiments, which is characterized in that it comprises an "L"-shaped constant temperature box, a reaction tank with connecting ring chambers at both ends, and a pair of reaction tanks supporting the reaction tank. The supporting seat and the transmission device that drives the reaction tank to rotate, among them, the "L" shaped box is divided into two parts, the box upright at the rear is the cold air box; the box at the front is the hot air box, and the cold (hot) air There is a cold (hot) air outlet with a valve on one side of the box, which is connected to a tee through the air guide pipe outside the box; the support seat is set on the hot gas box, one of which is fixed, and the other moves and locates relative to the fixed support seat. Active and passive rotary terminals are respectively connected to the support seat through bearings; the two rotary terminals are both thick in the middle and thin at both ends, and the center is hollow, and the opposite ends of the two rotary terminals are respectively inserted into the connecting ring cavities at both ends of the reaction tank for matching Sealing; one of the opposite ends of the two rotating terminals is the constant temperature gas inlet, which is connected to the third port of the tee through the air guide tube.

Description

A rotary shaker for microbiology and molecular biology experiments

technical field

The present invention relates to a device for clinical medicine and basic medical experiments, in particular to a multifunctional device for bacteria amplification in microbiology experiments and a multifunctional device for keeping reactants in a flowing state and fully combined in molecular biology experiments .

Background technique

In recent years, with the development and progress of the medical field, molecular biology and microbiology experimental techniques have been more widely used in scientific research, scientific researchers have more means to explore the mysteries of life science, and innovative ideas are becoming more and more Many, the experimental technology is becoming more and more mature, and the types of instruments required are becoming more and more diversified. In molecular biology experiments (such as westernblot and co-immunoprecipitation, etc.) and microbiological experiments (such as plasmid extraction), in order to promote bacterial amplification and make the reactants more fully combined, experimenters often need to use a special The unique device "shaker" keeps the reaction system in a flowing state. Currently, the shakers sold on the market include two types of flat plate rotation and spring swing, and they do not have the ability to be used under constant temperature and low temperature conditions. Therefore, the shaker has to be placed in the refrigerator (freezer). On the one hand, it takes up limited space resources in the box and cabinet. On the other hand, the external wires connected to the shaker easily lead to the lax closing of the cabinet door and affect the constant temperature effect. To meet the needs of the majority of scientific researchers for some special experiments.

Contents of the invention

Aiming at the problems existing in the existing shaker, the purpose of the present invention is to provide an economical and affordable rotary shaker capable of both high and low temperature, constant temperature and variable speed.

To achieve the above object, the present invention is achieved by taking the following technical solutions:

A rotary shaker for microbiology and molecular biology experiments, characterized in that it includes an "L"-shaped thermostatic box, a reaction tank with connecting ring chambers at both ends, a pair of support seats supporting the reaction tank, a drive The transmission device for the rotation of the reaction tank, wherein the "L"-shaped box is divided into two parts, the upright box at the rear is a cold air box; the flat front box is a hot air box, and a refrigeration device is installed in the cold air box. There is a cold air outlet with a valve on one side of the box, which is connected to a tee through an air pipe outside the box; a heating device is installed inside the hot air box, and a hot air outlet with a valve is provided on one side of the hot air box, which is connected to the air pipe outside the box. Three-way connection; the pair of support bases are arranged on the hot gas box, one of which is fixed, and the other is moved and positioned on the surface of the heating box through a slide rail relative to the fixed support base, and the active rotating terminal is connected to the fixed support base through a bearing ; On the opposite supporting seat, the passive rotary terminal is connected by a bearing. The main and passive rotary terminals are both thick in the middle and thin at both ends, and the center is hollow. In the connecting ring cavity connected in the tank, it is anastomosed and sealed; at the opposite end of the two rotating terminals, one is the constant temperature gas inlet and the other is the constant temperature gas outlet, wherein the constant temperature gas inlet passes through the air guide tube and the third port of the tee. connect.

In the above solution, the outlet of the constant temperature gas is directly connected to the atmosphere for discharge, or is recycled to the cold and hot boxes through the same pipeline as the intake system for recycling.

The transmission device includes that the active rotating terminal is provided with a ring groove and is connected with an output wheel of a variable speed motor arranged near the fixed support seat on the heating box through a transmission belt.

The reaction tank is made of insulating material into two half-cylinders that can be opened and closed. A half-ring cavity is arranged at the center of both ends of the half-cylinder. Connected connecting ring cavity.

The two half cylinders of the reaction tank, wherein a sealed bottle is axially arranged on the inner cylinder wall of one half cylinder, and a plurality of test tubes are axially arranged on the inner cylinder wall of the other half cylinder, and the sealed bottles or test tubes are connected to the tank through the holder. Internal wall connections.

The holder is made of elastic material and has a notched ring in section, and the ring bottom of the notch is fixed on the inner wall of the tank, and the distance between the notches is smaller than the diameter of the sealed bottle or test tube.

Heat dissipation holes and an air filter screen for gas exchange are left in front of the hot air box.

The invention has the advantages of being multi-functional, and can be used under high (low) temperature, constant temperature, and variable speed conditions at the same time, and has good constant temperature effect and simple operation, which meets the needs of scientific research workers for some special experiments.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure of the rotary shaker of the present invention.

Fig. 2 is a structural schematic diagram of the reaction tank in Fig. 1 .

Fig. 3 is a partial sectional view along the line A-A in Fig. 2 .

In Fig. 1 to Fig. 3: 1, cold air box; 2, cold air outlet; 3, constant temperature gas inlet; 4, hot air outlet; 5, transmission belt; 6, hot air box; 7, variable speed motor; 8, reaction tank; Device; 10. Support seat; 11. Constant temperature gas outlet; 12. Passive rotary terminal; 13. Refrigeration device; 14. Active rotary terminal; 15. Tee; 16. Semi-ring cavity; 17. Sealed bottle; ; 19, half cylinder; 20, test tube; 21. The inner wall of the tank.

Detailed ways

Referring to Fig. 1, a rotary shaker for microbiological and molecular biology experiments includes an "L"-shaped thermostatic box, a reaction tank 8 made of insulating material that can be opened and closed, and a pair of supports for supporting the reaction tank Seat 10, the transmission device that drives the reaction tank to rotate, the hot and cold air outlet of the thermostatic box, and the pipelines (not shown in the figure) connected to the hot and cold air outlet.

The "L"-shaped box is divided into two parts. The upright box at the rear is the cold air box 1, and the flat front box is the hot air box 6. The cold air box 1 is equipped with a refrigeration device 13 (compressor, evaporation and condensation) pipe, temperature sensor, etc.), one side of the air-conditioning box is provided with an air-conditioning outlet 2, on which a valve (not shown in the figure) can be arranged, and is connected with a tee 15 by an air guide pipe outside the box. Heating device 9 (heating element, fan, temperature sensor, etc.) On the same side) there is a hot gas outlet 4 on which a valve can be arranged, and is connected with the tee 15 by the air guide pipe outside the box.

A pair of supporting seats 10 supporting the reaction tank 8 are arranged on the hot gas box 6, one of which is fixed on the hot gas box, and the other supporting seat can be relatively fixed on the surface of the hot gas box along the X direction through a slide rail (not shown in the figure). The support seat moves and locates to facilitate installation and disassembly of the reaction tank 8. The active rotating terminal 14 is connected to the fixed support seat through a bearing; the passive rotating terminal is connected to the opposite support seat through a bearing. The active and passive rotary terminals 14 and 12 are both thick in the middle and thin at both ends, and the center is hollow. The opposite ends of the two rotary terminals are inserted into the connecting ring cavity at both ends of the reaction tank 8 (communicated with the inside of the tank) and coincide with each other. Sealing (refer to Figure 2); the opposite ends of the two rotating terminals are the constant temperature gas inlet 3 and the constant temperature gas outlet 11 respectively, wherein the constant temperature gas inlet 3 is connected to the third port of the tee 15 through the air guide tube. The constant temperature gas outlet 11 is directly connected to the air discharge, and can also be recovered to the cold air through the same method as the intake system (the cold and hot air inlets and valves are installed on the hot and cold air box, and the constant temperature gas outlet 11 is connected to the constant temperature gas outlet 11 through another tee and a conduit). , in a hot box for recycling.

The active rotating terminal 14 is provided with a ring groove and links to each other with an output wheel of a variable speed motor 7 that is arranged on the hot gas box (near the fixed support seat) through a transmission belt 5 (such as a belt). The variable speed motor 7 can adjust its rotational speed through a speed regulating switch to indirectly control the rotational speed of the reaction tank 8 .

Referring to Fig. 2, the reaction tank 8 is formed by two semi-cylindrical bodies 19 which are relatively airtightly connected (detachable). Annular cavity 16 forms a connecting annular cavity (communicating with the inside of the tank). A sealed bottle 17 is axially arranged on the inner cylinder wall of one of the half cylinders, and a plurality of test tubes 20 are axially arranged on the inner cylinder wall of the other half cylinder. The sealed bottle 17 and the test tube 20 can balance each other, reduce the rotation resistance of the reaction tank, and prolong the service life. Both the sealed bottle 17 and the test tube 20 can be conveniently connected with the tank inner wall 21 through the deck 18 .

Referring to Fig. 3, the holder 18 is made of elastic material (such as hard plastic rubber), and its section is a ring with a gap, and the bottom of the ring relative to the gap is fixed on the inner wall 21 of the tank, and the size of the gap should be smaller than that of a sealed bottle or test tube When the sealed bottle or test tube is just inserted into the holder from the gap, the gap is opened under pressure; when the sealed bottle or test tube enters the annular holder, the gap retracts so that the sealed bottle or test tube will not come out.

The working principle of the rotary shaker of the present invention is to install the reaction bottle and the test tube in the two half cylinders of the reaction tank 8, connect the two half cylinders 19 in a relatively sealed manner, and make a support seat on the surface of the hot gas box along the X side by a slide rail. The direction is moved and positioned relative to the fixed support seat, and the opposite ends of the two rotating terminals are inserted into the connecting ring cavity at both ends of the reaction tank 8; according to the needs of cold and heat experiments, open the cold (hot) gas outlet valve to make the cold (hot) The cold (hot) gas in the air box is poured into the reaction tank through the tee and the constant temperature gas inlet 3, so that the reaction bottle and the test tube in the tank maintain a constant temperature; start the variable speed motor 7, so that the reaction tank rotates at a certain speed The reaction system is always in a flowing state, and the reactants are more fully combined. The cold air box can provide 4°C constant temperature cold air in the reaction tank 8 to carry out the binding reaction between the tissue lysate and the antibody in the immunoprecipitation reaction or the binding reaction between the PVDF membrane and the primary antibody in the western blot experiment. The hot air box can provide 37°C constant-temperature hot air in the reaction tank 8 to carry out the plasmid amplification reaction in the bacteria in the molecular cloning or the sealing of the PVDF membrane in the western blot test. And the speed of the reaction tank 8 can be controlled at 50-100 revolutions per minute through the variable speed motor 7, so as to meet the needs of different experiments.

Claims (7)

1. rotary shaker that is used for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there are retort, a pair of supporting base that supports this retort that connects ring cavity, the transmission device that drives the retort rotation in " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is the hot gas case, is provided with refrigerating plant in the cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; Be provided with heating combined equipment in the hot gas case, hot gas case one side is provided with one with the heat outlet of valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above the hot gas case, and one of them is fixed, and another moves and locate heating the relatively-stationary supporting base in case surface by slide rail, connects the active rotation terminal by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base, main, passive rotary terminal is thin ends and thick middle structure, the center is hollow, and the sealing that coincide in the connection ring cavity at retort two ends of pegging graft respectively of two a rotary terminals end in opposite directions connects ring cavity and is communicated with tank is interior; The end that two rotary terminals are opposing, one is the constant temperature gas feed, and another is the constant temperature gas vent, and wherein, the constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.

2. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1 is characterized in that described constant temperature gas vent directly is communicated with atmosphere, or is recycled in the hot and cold case by the pipeline mode identical with gas handling system, carries out recycling.

3. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, described transmission device comprises, the active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near the variable speed electric motors, particularly the fixed mounting base on the case and links to each other with one by driving-belt.

4. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, but described retort is made two and half cylindrical shells of folding by insulation material, center, half cylindrical shell two ends is provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively, half ring cavity at two ends form one with tank in connection be connected ring cavity.

5. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 4, it is characterized in that, two and half cylindrical shells of described retort, on one of them half cylindrical shell inner tube wall an air-tight bottle is set axially, on another half cylindrical shell inner tube wall a plurality of test tubes are set axially, air-tight bottle or test tube are connected with inner tank wall by deck.

6. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 5, it is characterized in that, it is a notched ring-type that described deck is made the cross section by elastomeric material, relatively is fixed on the inner tank wall at the bottom of the ring of breach, and this breach spacing is less than the diameter of air-tight bottle or test tube.

7. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1 is characterized in that louvre and the airstrainer for the gas exchange left in described hot gas case front.

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