CN102961998A - Rotary shaker for microorganism and molecular biological experiments - Google Patents
Rotary shaker for microorganism and molecular biological experiments Download PDFInfo
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- CN102961998A CN102961998A CN2012104840678A CN201210484067A CN102961998A CN 102961998 A CN102961998 A CN 102961998A CN 2012104840678 A CN2012104840678 A CN 2012104840678A CN 201210484067 A CN201210484067 A CN 201210484067A CN 102961998 A CN102961998 A CN 102961998A
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Abstract
The invention discloses a rotary shaker for microorganism and molecular biological experiments. The rotary shaker is characterized by comprising an L-type constant temperature tank body, a reaction pot, a pair of support seats, and a drive device for driving the reaction pot to rotate, wherein two ends of the reaction pot are connected with ring cavities; the pair of support seats supports the reaction pot; the L-type tank body is divided into two parts; a vertical tank body at the rear part is a cold air box; a horizontal tank body at the front part is a hot air box; a cold (hot) air outlet with a valve is arranged in one side of the cold (hot) air box, and connected with a tee joint through an air duct outside the box; the support seats are arranged on the hot air box, one support seat is fixed and the other support seat is relatively moved and located; the support seats are respectively connected with a driving rotary terminal and a driven rotary terminal through bearings; the two rotary terminals are of structures in which the middle parts are thick, two ends are thin, and the centers are hollow; the opposite ends of the two rotary terminals are respectively inserted into the ring cavities connected to two ends of the reaction pot to be coincided and sealed; and one of the reversed ends of the two rotary terminals is a constant-temperature air inlet, and the constant-temperature air inlet is connected with a third outlet of the tee joint through the air duct.
Description
Technical field
The present invention relates to a kind of device for clinical medicine and Basic medicine experiment, particularly make reactant be in all the time under the flow regime and well-bound multi-function device in a kind of bacterium amplification for Experiment on Microbiology, the molecular biosciences experiment.
Background technology
In recent years, development and progress along with medical domain, molecular biology and the application of Microbiology Experiment technology in research work are more extensive, the secret that researcher is explored life science has had more abundant means, innovative idea is more and more many, experimental technique is ripe day by day, and required instrument kind is tending towards variation.In molecular biology experiment (such as westernblot and co-immunoprecipitation etc.) and Experiment on Microbiology (for example plasmid extraction), in order to impel bacterium amplification, to make more sufficient combination is arranged between the reactant, the experimenter often need to utilize a kind of device " shaking table " of special use, makes reaction system be in all the time flow regime.The shaking table of selling in the market comprises two kinds of dull and stereotyped rotary and Spring Pendulum dynamic formulas, and they do not possess under constant temperature, cryogenic conditions uses.Therefore have to shaking table is placed refrigerator (refrigerator), this has taken limited space resources in case, the cabinet on the one hand, affect the constant temperature effect owing to the shaking table external cord easily causes cabinet door to be not closed completely on the other hand, can not satisfy vast researcher to the needs of some special experiment.
Summary of the invention
For the existing problem of existing shaking table, the purpose of this invention is to provide a kind of can high low temperature economical and practical type rotary shaker dual-purpose, the constant temperature variable-ratio.
For reaching above purpose, the present invention takes following technical scheme to be achieved:
A kind of rotary shaker for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there are retort, a pair of supporting base that supports this retort that connects ring cavity, the transmission device that drives the retort rotation in " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is the hot gas case, is provided with refrigerating plant in the cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; Be provided with heating combined equipment in the hot gas case, hot gas case one side is provided with one with the heat outlet of valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above the hot gas case, and one of them is fixed, and another moves and locate heating the relatively-stationary supporting base in case surface by slide rail, connects the active rotation terminal by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base, main, passive rotary terminal is thin ends and thick middle structure, the center is hollow, and two a rotary terminals end is in opposite directions pegged graft respectively retort two ends and interior being connected in the ring cavity of being communicated with of tank, and the sealing that coincide; The end that two rotary terminals are opposing, one is the constant temperature gas feed, and another is the constant temperature gas vent, and wherein, the constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.
In the such scheme, described constant temperature gas vent directly is communicated with airborne release, or is recycled in the hot and cold case by the pipeline mode identical with gas handling system, carries out recycling.
Described transmission device comprises, the active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near the variable speed electric motors, particularly the fixed mounting base on the case and links to each other with one by driving-belt.
But described retort is made two and half cylindrical shells of folding by insulation material, and center, half cylindrical shell two ends is provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively half ring cavity at two ends form one with tank in connection be connected ring cavity.
Two and half cylindrical shells of described retort axially arrange an air-tight bottle on one of them half cylindrical shell inner tube wall, on another half cylindrical shell inner tube wall a plurality of test tubes are set axially, and air-tight bottle or test tube are connected with inner tank wall by deck.
It is a notched ring-type that described deck is made the cross section by elastomeric material, relatively is fixed on the inner tank wall at the bottom of the ring of breach, and this breach spacing is less than the diameter of air-tight bottle or test tube.
Louvre and the airstrainer for the gas exchange left in described hot gas case front.
Advantage of the present invention is, and is multi-functional, can under high (low) temperature, constant temperature, Variable Velocity Condition, use simultaneously, and good constant-temperature effect, simple to operate, satisfy researcher to the needs of some special experiment.
Description of drawings
Fig. 1 is rotary shaker overall structure schematic diagram of the present invention.
Fig. 2 is the structural representation of retort among Fig. 1.
Fig. 3 be among Fig. 2 A-A to partial sectional view.
Among Fig. 1 to Fig. 3: 1, cold air case; 2, cold air outlet; 3, constant temperature gas feed; 4, heat outlet; 5, driving-belt; 6, hot gas case; 7, variable speed electric motors, particularly; 8, retort; 9, heater; 10, supporting base; 11, constant temperature gas vent; 12, passive rotary terminal; 13, refrigerating plant; 14, active rotation terminal; 15, threeway; 16, half ring cavity; 17, air-tight bottle; 18, deck; 19, half cylindrical shell; 20, test tube; 21.Inner tank wall.
The specific embodiment
With reference to figure 1, a kind of rotary shaker for the experiment of microorganism and molecular biosciences, comprise " L " type thermostatic box, one by insulation material make can folding retort 8, a pair of supporting base 10 that supports this retort, the cold and hot gas outlet of transmission device, thermostatic box that drives the retort rotation and the pipeline (not shown in FIG.) that connects cold and hot gas outlet.
" L " molding box body is divided into two parts, the upright casing in rear portion is cold air case 1, the casing of anterior horizontal is hot gas case 6, be provided with refrigerating plant 13(compressor, evaporation and condenser pipe, temperature sensor etc. in the cold air case 1), cold air case one side is provided with cold air outlet 2, valve (not shown in FIG.) can be set on it, be connected with a threeway 15 by the outer wireway of case.Heating combined equipment 9(heater element, fan, temperature sensor etc. are housed) in the hot gas case 6, louvre and the airstrainer (not shown in FIG.) for the gas exchange left in the front, hot gas case one side (with cold air outlet 2 homonymies) has a heat outlet 4, on it valve can be set, by the outer wireway of case with threeway 15 be connected.
The a pair of supporting base 10 that supports retort 8 is arranged on above the hot gas case 6, one of them is fixed on the hot gas case, another supporting base is by slide rail (not shown in FIG.), can be on hot gas case surface move and locate relative to fixed mounting base along directions X, with conveniently install, dismounting retort 8.Connect active rotation terminal 14 by bearing on the fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base.Main, passive rotary terminal 14,12 is thin ends and thick middle structure, and the center is hollow, and two a rotary terminals end is in opposite directions pegged graft (with being communicated with in the tank) in the connection ring cavity at retort 8 two ends, and the sealing (with reference to figure 2) that coincide; The opposing end of two rotary terminals is respectively constant temperature gas feed 3, constant temperature gas vent 11, and wherein, constant temperature gas feed 3 is connected with the 3rd mouth of threeway 15 by wireway.Constant temperature gas vent 11 directly is communicated with airborne release, also can be recycled in the hot and cold case by the mode identical with gas handling system (hot and cold gas import and valve are set on the cold and hot gas tank, also connect constant temperature gas vent 11 by another threeway and conduit), carry out recycling.
With reference to figure 2, retort 8 relatively is tightly connected by two and half cylindrical shells 19 and forms (removable), and half ring cavity 16 at two ends formed one and connects ring cavity (with being communicated with in the tank) when 16, two half cylindrical shells of half ring cavity that center, half cylindrical shell two ends arranges were tightly connected relatively.On one of them half cylindrical shell inner tube wall an air-tight bottle 17 is set axially, on another half cylindrical shell inner tube wall a plurality of test tubes 20 is set axially.Air-tight bottle 17, test tube 20 be balance mutually, reduces the retort rotational resistance, increases the service life.Air-tight bottle 17, test tube 20 can conveniently be connected with inner tank wall 21 by deck 18.
With reference to figure 3, deck 18 is made (such as hard plastic rubber) by elastomeric material, its cross section is a notched ring-type, relatively be fixed on the inner tank wall 21 at the bottom of the ring of breach, the breach size should be less than the diameter of air-tight bottle or test tube, when air-tight bottle or test tube had just embedded deck from indentation, there, this breach pressurized opened; After air-tight bottle or test tube entered in the ring-type deck, the indentation, there retraction can not deviate from air-tight bottle or test tube.
The operation principle of rotary shaker of the present invention is, in 8 two half cylindrical shells of retort, install reaction bulb and test tube, two and half cylindrical shells 19 are tightly connected relatively, make a supporting base move and locate relative to fixed mounting base along directions X on hot gas case surface by slide rail, two a rotary terminals end is in opposite directions pegged graft in the connection ring cavity at retort 8 two ends; Select to open cold (heat) gas outlet valve according to hot and cold experiment needs, cold (heat) gas in cold (heat) gas tank is poured in the retort by threeway and constant temperature gas feed 3, make in the tank reaction bulb and test tube keep constant temperature; Start variable speed electric motors, particularly 7, make retort by certain speed rotation, under constant temperature, make reaction system be in all the time flow regime, more sufficient combination is arranged between the reactant.Cold air case can be provides 4 ℃ constant temperature cold air in the retort 8, carry out organizing in the immune precipitation pvdf membrane and primary antibodie association reaction in lysate and antibody association reaction or the western blot experiment.The hot gas case can be provides 37 ℃ of constant temperature hot gas in the retort 8, carry out the sealing of pvdf membrane in plasmid amplified reaction in the bacterium in the molecular cloning or the western blot test.And the rotating speed of retort 8 can be controlled at per minute 50 ~ 100 by variable speed electric motors, particularly 7 and turn, to satisfy the needs of different experiments.
Claims (7)
1. rotary shaker that is used for microorganism and molecular biosciences experiment, it is characterized in that, comprise that there are retort, a pair of supporting base that supports this retort that connects ring cavity, the transmission device that drives the retort rotation in " L " type thermostatic box, two ends, wherein, " L " molding box body is divided into two parts, and the upright casing in rear portion is cold air case; The casing of anterior horizontal is the hot gas case, is provided with refrigerating plant in the cold air case, and cold air case one side is provided with the cold air outlet with valve, is connected with a threeway by the outer wireway of case; Be provided with heating combined equipment in the hot gas case, hot gas case one side is provided with one with the heat outlet of valve, is connected with threeway by the outer wireway of case; Described a pair of supporting base is arranged on above the hot gas case, and one of them is fixed, and another moves and locate heating the relatively-stationary supporting base in case surface by slide rail, connects the active rotation terminal by bearing on fixed mounting base; Connect passive rotary terminal by bearing on another relative supporting base, main, passive rotary terminal is thin ends and thick middle structure, the center is hollow, and the sealing that coincide in the connection ring cavity at retort two ends of pegging graft respectively of two a rotary terminals end in opposite directions connects ring cavity and is communicated with tank is interior; The end that two rotary terminals are opposing, one is the constant temperature gas feed, and another is the constant temperature gas vent, and wherein, the constant temperature gas feed is connected with the 3rd mouth of threeway by wireway.
2. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1 is characterized in that described constant temperature gas vent directly is communicated with atmosphere, or is recycled in the hot and cold case by the pipeline mode identical with gas handling system, carries out recycling.
3. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, described transmission device comprises, the active rotation terminal is provided with annular groove and is arranged on the output wheel that heats near the variable speed electric motors, particularly the fixed mounting base on the case and links to each other with one by driving-belt.
4. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 1, it is characterized in that, but described retort is made two and half cylindrical shells of folding by insulation material, center, half cylindrical shell two ends is provided with half ring cavity, when two and half cylindrical shells are tightly connected relatively, half ring cavity at two ends form one with tank in connection be connected ring cavity.
5. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 4, it is characterized in that, two and half cylindrical shells of described retort, on one of them half cylindrical shell inner tube wall an air-tight bottle is set axially, on another half cylindrical shell inner tube wall a plurality of test tubes are set axially, air-tight bottle or test tube are connected with inner tank wall by deck.
6. the rotary shaker for the experiment of microorganism and molecular biosciences as claimed in claim 5, it is characterized in that, it is a notched ring-type that described deck is made the cross section by elastomeric material, relatively is fixed on the inner tank wall at the bottom of the ring of breach, and this breach spacing is less than the diameter of air-tight bottle or test tube.
7. the rotary shaker for microorganism and molecular biosciences experiment as claimed in claim 1 is characterized in that louvre and the airstrainer for the gas exchange left in described hot gas case front.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210484067.8A CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210484067.8A CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
Publications (2)
| Publication Number | Publication Date |
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| CN102961998A true CN102961998A (en) | 2013-03-13 |
| CN102961998B CN102961998B (en) | 2014-10-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210484067.8A Expired - Fee Related CN102961998B (en) | 2012-11-23 | 2012-11-23 | Rotary shaker for microorganism and molecular biological experiments |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141746A (en) * | 2019-06-18 | 2019-08-20 | 河南省中医院（河南中医药大学第二附属医院） | Breathe internal medicine with helping inhaling ware |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87211803U (en) * | 1987-08-10 | 1988-09-28 | 任克宁 | Speed-adjustable rotary culture device |
| WO1998041837A1 (en) * | 1997-03-17 | 1998-09-24 | Iliya Borisovich Izvozchikov | Device for the processing and infiltration of histological specimens |
| WO2000060362A1 (en) * | 1999-04-08 | 2000-10-12 | Chung Chang Young | Apparatus for fast preparation and analysis of nucleic acids |
| CN1290743A (en) * | 1999-11-16 | 2001-04-11 | 中国科学院力学研究所 | Three-dimensional discal rotary hollow on-line cell cultivator with air and liquid supply |
| CN201676659U (en) * | 2010-05-24 | 2010-12-22 | 杭州奥盛仪器有限公司 | Constant temperature blending instrument |
| CN203002284U (en) * | 2012-11-23 | 2013-06-19 | 西安医学院第二附属医院 | Rotary table for experiments on micro-organisms and molecular organisms |
-
2012
- 2012-11-23 CN CN201210484067.8A patent/CN102961998B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87211803U (en) * | 1987-08-10 | 1988-09-28 | 任克宁 | Speed-adjustable rotary culture device |
| WO1998041837A1 (en) * | 1997-03-17 | 1998-09-24 | Iliya Borisovich Izvozchikov | Device for the processing and infiltration of histological specimens |
| WO2000060362A1 (en) * | 1999-04-08 | 2000-10-12 | Chung Chang Young | Apparatus for fast preparation and analysis of nucleic acids |
| CN1290743A (en) * | 1999-11-16 | 2001-04-11 | 中国科学院力学研究所 | Three-dimensional discal rotary hollow on-line cell cultivator with air and liquid supply |
| CN201676659U (en) * | 2010-05-24 | 2010-12-22 | 杭州奥盛仪器有限公司 | Constant temperature blending instrument |
| CN203002284U (en) * | 2012-11-23 | 2013-06-19 | 西安医学院第二附属医院 | Rotary table for experiments on micro-organisms and molecular organisms |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141746A (en) * | 2019-06-18 | 2019-08-20 | 河南省中医院（河南中医药大学第二附属医院） | Breathe internal medicine with helping inhaling ware |
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| Publication number | Publication date |
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| CN102961998B (en) | 2014-10-22 |
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