CN102949473A - Preparation method of pullingqianzheng pills - Google Patents
Preparation method of pullingqianzheng pills Download PDFInfo
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- CN102949473A CN102949473A CN2012104930994A CN201210493099A CN102949473A CN 102949473 A CN102949473 A CN 102949473A CN 2012104930994 A CN2012104930994 A CN 2012104930994A CN 201210493099 A CN201210493099 A CN 201210493099A CN 102949473 A CN102949473 A CN 102949473A
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Abstract
The invention discloses a preparation method of pullingqianzheng pills, and the preparation method is characterized in that 3 to 5 parts by weight of rhizoma typhonii, 3 to 5 parts by weight of stiff silkworm and 4 to 6 parts by weight of scorpio are adopted as crude drugs, and the crude drugs are sorted to remove the impurities; the scorpio is dried after being broken; the crude drugs are shattered into fine powder through an ultrafine pulverizer to be sterilized; the ultrafine powder of the crude drugs is mixed with yellow wine suspension to prepare softwood; the softwood is prepared into pills through plastic method, and then the pills are coated after being dried under the temperature of 50 to 80DEG C; and the pills are dried after being coated to obtain the pullingqianzheng pills. Due to the adoption of the preparation method, not only can biological active ingredients of the pullingqianzheng pills be maintained, but also the toxicity of the calcium oxalate crystal which is the toxic component in the rhizome typhonii can be reduced. The prepared pullingqianzheng pills hasve the advantages of high treatment effect, small dosage, small toxicity and side effect and convenience in use.
Description
Technical field
The invention belongs to the Chinese medicine preparation technical field, relate to a kind of preparation method of leading positive ball.
Background technology
Think that QIANZHENGSAN comes from Song dynasty poplar Dan " Young man Tibetan side " in the most literature He in the textbook, consist of Rhizoma Typhonii, Bombyx Batryticatus, Scorpio 3 flavor medicines.In fact, before the Song dynasty, the treatment facial hemiparalysis is just arranged in the Tang Dynasty Sun Simiao " prescriptions worth thousand gold ", and it " does not change Jindan ", has used living Rhizoma Typhonii, Bombyx Batryticatus; In three Wu are loose, used Scolopendra, Rhizoma Arisaematis, the Radix Angelicae Dahuricae and Moschus moschiferous fragrant; Claim in gold dollar Zhu Dan in period small stream " danxi's experiential therapy " book: " if apoplexy, facial hemiparalysis, Rhizoma Typhonii, Bombyx Batryticatus, Scorpio, and give birth to usefulness, each five equilibrium grinds and is the end, each money, hot wine is transferred lower." visible we are ripe Tang, Song, gold dollar period, and sometimes add Scolopendra later age, or change the loose soup that is, or add Rhizoma Arisaematis, the Rhizoma Pinelliae, Zaocys, Agkistrodon etc. in the side, use until modern age clinical.
QIANZHENGSAN cures mainly facial paralysis-facial paralysis.Because there is defective in the record aspect to apoplexy dough-making powder paralysis in the successive dynasties Chinese medicine document, comparatively rough on some words and phrases is described, therefore cause the mistaken ideas on some understanding.Should refer to peripheral facial paralysis as " facial hemiparalysis " is many, and apoplexy sequela is central facial palsy, performance should be " distortion of commissure ", and partially askew can accompany simultaneously aphasis, dysphagia or tongue body to stretch out the time, but does not affect catacleisis.Even peripheral facial paralysis shows as facial hemiparalysis, but also bell palsy is arranged, HH and traumatic secondary reason cause and perform the operation, inject the operations such as sealing, maternal infuries and internal ear, mastoid process position and cause.
Therefore one of mistaken ideas are to think that QIANZHENGSAN can treat peripheral facial paralysis, claim such as Song Yanyong and recipes for Saving Lives: " be hemiplegia, myalgia; For sputum is jammed, mouthful eye fish sticking its mouth out of the water tiltedly, hemiplegia, mind is dazed and confused." first Li Gao " eastern wall ten books " medium cloud wind: " invasion of vessels is mouthful eye then
Tiltedly." bright Lou Ying (1332-1402 A.D) " Compendium of medicine " cloud: " all hemiplegia persons must mouthful eye
Tiltedly." clear Wu Kun " hospital examines " cloud: " apoplexy, mouthful eye
Tiltedly, without he card person, this side master's." clear what dream precious jade " doctor's rock " cloud: " general QIANZHENGSAN.”
Until today, all chance peripheral facial paralysiies of many clinicists, pay no attention to dialectical, first-selected QIANZHENGSAN.Every side must Rhizoma Typhonii, Bombyx Batryticatus, Scorpio, Scolopendra, Zaocys, Agkistrodon and so on, but therapeutic effect is unsatisfactory.Trace it to its cause, the one, QIANZHENGSAN is the prescription for the treatment of apoplexy central facial palsy, shall not be applied to peripheral facial paralysis.Clear Wu Kun " hospital examines " claims: " ... the Rhizoma Typhonii acrid in the mouth, can make wind dispelling, silkworm, scorpion salty can make soft expectorant; Heat is arranged in the suffering, can make from wind, silkworm, scorpion are poisonous, can make brokenly knot, doctor's medication, and useful its heat is to attack heat, and with the counteracting toxic substances person, " Da Yi " is so-called, and People of the same tastes and habits like to be together with its poison, and " interior warp " so-called proper eliminating method according to the nature of pathogen is also."; The 2nd, great majority are bell palsy in the peripheral facial paralysis, and are how relevant with viral infection, and the lighter that falls ill only has the characteristics of exterior syndrome due to wind-cold, and mastoid process place or half side headache, prosopodynia or hyperacusis behind the ear, tinnitus, Hearing change can appear in Most patients; Or the Ipsilateral conjunctival congestion, shed tears, or front 2/3 hypogeusia of tongue, show as wind-heat troubling, liver and gall intenseness of heat, or the acute inflammation performance such as excessive noxious heat; Later stage can show as deficient vital QI leading to lingering of pathogen, the not flourish virtual image that waits of insufficiency of vital energy and blood, facial muscle, and above dialectical situation all non-QIANZHENGSAN institute is suitable; The 3rd, peripheral facial paralysis shows as face paralysis, paralysis character is from the beginning of being flaccid paralysis, facial muscle lost-motion function but not spasm, and the main effect of QIANZHENGSAN is relieving spasm by subduing liver-wind, can aggravate of flaccid muscles, so QIANZHENGSAN should not be used for the early stage of peripheral facial paralysis.
QIANZHENGSAN tradition usage is Rhizoma Typhonii, Bombyx Batryticatus, each five equilibrium of Scorpio, grinds to be the end, and each money, hot wine are transferred lower.In the clinical practice, the patient uses very inconvenience: be difficult for porphyrize, affect the performance of drug effect; When the patient does not have alcohol drinking patterns, affect the application of extra conductant ingredient; The urine smell abnormal smells from the patient of Bombyx Batryticatus, Scorpio animal drugs affects patient's compliance.
Summary of the invention
The problem that the present invention solves is to provide a kind of preparation method of leading positive ball, both kept the bioactive ingredients that leads positive ball, reduced again the toxicity of the toxic component needle-like calcium oxalate crystal in the Rhizoma Typhonii, prepared led positive ball and have that curative effect is high, dosage is little, toxic and side effects is little, the advantage of taking convenience.
The present invention is achieved through the following technical solutions:
A kind of preparation method of leading positive ball may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 3～5 parts Rhizoma Typhoniis, 3～5 parts Bombyx Batryticatus and 4～6 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, after preliminary the pulverizing, adopt again super micron mill to be ground into impalpable powder, then sterilization;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 3～10:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 3～10% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 50～80 ℃ of dryings; Dry again after coating is finished, obtain leading positive ball.
Further, in mass fraction, with the Scorpio of 3.5～3.92 parts Rhizoma Typhoniis, 3.2～3.84 parts Bombyx Batryticatus and 4.27～5.0 parts as crude drug.
Lower dry at 50～80 ℃ after the described Scorpio fragmentation.
The needle-like calcium oxalate crystal that does not contain Rhizoma Typhonii in the described impalpable powder.
Cell wall breaking rate＞95% in the described impalpable powder.
The fineness of described impalpable powder is controlled to be can pass through 200 mesh sieves, sterilizes after crude drug was crossed 200 mesh sieves after super micron mill was pulverized.
Described sterilization is to use cobalt
60Radiation sterilization.
Described impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 5:1, and the mass concentration of cross-linking sodium carboxymethyl cellulose is 5% in the yellow wine suspension.
Described coating is to carry out film coating to leading positive ball.
The described ball weight-normality lattice that lead positive ball are 0.1g.
Compared with prior art, the present invention has following useful technique effect:
1, the present invention adopts superfine communication technique, reaches cell wall breaking.
The positive ball that leads of the present invention preparation had both kept the bioactive ingredients that leads positive ball, had reduced again the toxicity of the toxic component needle-like calcium oxalate crystal in the Rhizoma Typhonii, prepared led positive ball and had that curative effect is high, dosage is little, and toxic and side effects is little, the advantage of taking convenience.
When the pulverizing of crude drug, adopt the micronizing new technique, make crude drug cell sporoderm-broken rate＞95%; Cell is behind breaking cellular wall, effective ingredient can fully come out in the born of the same parents, make be stored in the cell and intercellular effective ingredient directly and the body receptors bind, be conducive to lead release and the absorption of positive ball, improved bioavailability, improve the medical material utilization rate, reached the homogenization of each composition, effectively kept the bioactive ingredients that leads positive ball.And with after the toxic component needle-like calcium oxalate crystal fragmentation in the Rhizoma Typhonii, reduced its toxicity.
2, eliminated the zest toxicity of Rhizoma Typhonii
The Rhizoma Typhonii that leads in the positive ball is one of 28 kinds of malicious drastic of State Council's announcement, and Rhizoma Typhonii calcium oxalate crystal growth in healthy needle is its irritant toxic component.The Rhizoma Typhonii toxic and side effects is mainly manifested in the strong and stimulating effect to mucosas such as oral cavity, throat, eye, stomaches, with document record have that numb feeling in the tongue is subjected to, clinical experience has the toxicity of " halberd people pharynx " to conform to, after finding the mouse peritoneal drug administration by injection in the experiment, all occur being slow in action, roll up, the high dose group of phenomenon, the especially needle of anorexia can see having that health trembles, Tachypneic performance.Can find out the LD of Rhizoma Typhonii fecula and its needle
50Value is respectively 2875 and 21.6mg/kg, and the toxicity of needle is more than 100 times that Rhizoma Typhonii is given birth to product.
The present invention has adopted micronizing to prepare impalpable powder, has eliminated Rhizoma Typhonii calcium oxalate crystal growth in healthy needle, is carrying out after the micronizing, and microscopically is inspected needle-like calcium oxalate crystal, has proved conclusively not contain needle-like calcium oxalate crystal in the impalpable powder; So just eliminate the needle-like calcium oxalate crystal in the Rhizoma Typhonii, reduced its toxicity, guaranteed the safety of clinical application.
3, lead the dissolution rate that has improved effective ingredient after the positive ball micronizing
The TLC collection of illustrative plates shows, under same extracting method, lead the common fine powder of positive ball and in identical retention time identical absworption peak is arranged with superfine powder, just peak value has difference, illustrate that Chinese crude drug does not have novel substance to generate after micronizing, the variation of matter does not occur in the intrinsic pharmacodynamic basis material of Chinese medicine.
After supersound extraction, lead the stripping quantity of positive ball mesoxalic acid ammonium, superfine powder is higher by 73.44% than common flour respectively, illustrates after the micronizing that wherein the burst size of effective ingredient increases considerably.Effectively reduce dosage, thereby reduce cost, economize on resources.Superfine powder is accelerated greatly than the dissolution rate of common flour, lead positive ball micronizing after, ammonium oxalate amount of stripping in 5min has substantially exceeded the maximum total amount of common flour stripping.Reason gives the credit to that grain size of micropowder is little, and the cell wall breaking rate is high, and stripping is not subjected to the obstruction of cell wall and cell membrane, so that the effective ingredient stripping quantity increases quickening.
4, lead the broken drug effect that improved of Scorpio superfine powder in the positive ball
The ischemic animal number average that Scorpio different-grain diameter superfine powder (1,5,10 μ m) group electrocardiogram occurs significantly is lower than model control group, shows that the myocardial ischemia that Scorpio different-grain diameter superfine powder causes pituitrin has obvious protection and improvement effect.
5, leading positive ball micronizing, to reduce stomach residual, is conducive to the drug effect performance
The drug effect of leading positive ball ultrafine powder traditional Chinese medicine suspendible group high dose group obviously is better than traditional decoction pieces water decoction group, lead positive ball ultrafine powder traditional Chinese medicine suspendible group low dose group and QIANZHENGSAN tradition decoction pieces water decoction group close, illustrate that micronizing Chinese medicine only needs 1/8 dosage can reach the drug effect of traditional decoction pieces water decoction, micronizing has greatly improved bioavailability and the drug effect of leading positive ball.
6, the general ball of yellow wine is covered bad smell, abides by ancient preparation, and drug effect is definite
Select the water pill agent, both abided by ancient the process of preparing Chinese medicine, production technology and traditional handicraft are basically identical; Consider again patient's taking convenience, curative effect is high, dosage is little to reach, and toxic and side effects is little, accumulating, carry, principle easy to use, adopt micronizing, the general ball of yellow wine is made water pill, has following advantage:
(1) the water pill grain is small, and specific surface area is large, and the ingredient stripping is very fast, and is rapid-action.
(2) watered pill coated after, profile rounding light, moistureproof, cover offending fishy smell.
(3) through the micronized watered pill, after the gastrointestinal disintegrate, distribution area is large, and bioavailability is high.
(4) water pill good fluidity, size is even, and divided dose is accurate.
(5) adopt the general ball of yellow wine, the extra conductant ingredient that had both kept the yellow wine of traditional usage to take after mixing it with water has been covered again the distinctive unhappy fishy smell of animal drugs.
The specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment, and the explanation of the invention is not limited.
Embodiment 1
A kind of preparation method of leading positive ball may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 3 parts Rhizoma Typhoniis, 3 parts Bombyx Batryticatus and 4 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, preliminary pulverize (or coarse powder) adopts super micron mill to be ground into impalpable powder, then sterilization again;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 3～10:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 5% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 50～60 ℃ of dryings; Drying after coating is finished obtains leading positive ball.
Embodiment 2
A kind of preparation method of leading positive ball may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 3.5 parts Rhizoma Typhoniis, 3.2 parts Bombyx Batryticatus and 5.0 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, preliminary pulverize (or coarse powder) adopts super micron mill to be ground into impalpable powder again, crosses 200 mesh sieves, then cobalt
60Radiation sterilization;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 10:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 4.5% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 60～70 ℃ of dryings; Drying after coating is finished obtains leading positive ball.
Embodiment 3
A kind of preparation method of leading positive ball may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 3.92 parts Rhizoma Typhoniis, 3.84 parts Bombyx Batryticatus and 4.27 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, preliminary pulverize (or coarse powder) adopts super micron mill to be ground into impalpable powder again, crosses 200 mesh sieves, then cobalt
60Radiation sterilization;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 10:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 6% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 70～76 ℃ of dryings; Drying after coating is finished obtains leading positive ball.
Embodiment 4
A kind of preparation method of leading positive ball may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 5 parts Rhizoma Typhoniis, 5 parts Bombyx Batryticatus and 6 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, preliminary pulverize (or coarse powder) adopts super micron mill to be ground into impalpable powder again, crosses 200 mesh sieves, then cobalt
60Radiation sterilization;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 3:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 3% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 70～80 ℃ of dryings; Drying after coating is finished obtains leading positive ball.
The prepared positive ball that leads has been carried out following drug effect comparative study:
1, leading positive ball micronizing, to reduce stomach residual, is conducive to the drug effect performance.
Take gastric emptying as observation index, lead the relevant comparative study of positive ball.50 ICR mices are divided into 5 groups at random, wherein 1 group is the blank group, 2 groups is QIANZHENGSAN tradition decoction pieces water decoction group, 3,4,5 groups for leading high, medium and low 3 the dosage groups of positive ball ultrafine powder traditional Chinese medicine suspendible group, the blank group, the isometric distilled water of gavage leads positive ball ultrafine powder traditional Chinese medicine suspendible group 25%, 12.5%, 6.25%, is basic, normal, high dosage group; QIANZHENGSAN tradition decoction pieces water decoction group 50%;
Water 12h is can't help in fasting before the experiment.Every group of relative medicine and distilled water that gavages respectively 0.24ml.30min only pours into the semi-solid 0.3ml/ of paste again after the perfusion.Take off cervical vertebra behind the 20min and put to death mice, cut open the belly immediately, ligation stomach cardia and pylorus take by weighing stomach full weight and net weight, calculate the gastric residual rate.Gastric residual rate=[0.8-(stomach full weight-stomach net weight)]/0.8x100%, experimental result is analyzed with the t inspection statistics, and testing result is as shown in table 1.
Table 1 leads the impact of residual rate in the positive ball micronizing Mouse Stomach
Annotate: compare ， ﹡ P＜0.05 ， ﹡ P＜0.01 with the blank group; With lead positive ball Chinese medicine decoction pieces water
The decoct group compares, #P＜0.05.
The result shows: lead positive ball ultrafine powder traditional Chinese medicine suspendible group and QIANZHENGSAN tradition decoction pieces water decoction group relatively, the Stomach residue rate of QIANZHENGSAN tradition decoction pieces water decoction group (concentration 50%) is equivalent to lead positive ball ultrafine powder traditional Chinese medicine suspendible low dose group (concentration 6.25%) (P＞0.05), leads positive ball ultrafine powder traditional Chinese medicine suspendible high dose group (concentration 25%) Stomach residue rate and significantly is lower than its traditional decoction pieces water decoction group (concentration 50%) (P＜0.05); As seen, the drug effect of leading positive ball ultrafine powder traditional Chinese medicine suspendible group high dose group obviously is better than traditional decoction pieces water decoction group, lead positive ball ultrafine powder traditional Chinese medicine suspendible group low dose group and QIANZHENGSAN tradition decoction pieces water decoction group close, illustrate that micronizing Chinese medicine only needs 1/8 dosage can reach the drug effect of traditional decoction pieces water decoction, micronizing has greatly improved bioavailability and the drug effect of leading positive ball.
2, lead the zest toxicity that positive ball has been eliminated Rhizoma Typhonii
The Rhizoma Typhonii that leads in the positive ball is one of 28 kinds of malicious drastic of State Council's announcement, and Rhizoma Typhonii calcium oxalate crystal growth in healthy needle is its irritant toxic component.The Rhizoma Typhonii toxic and side effects is mainly manifested in the strong and stimulating effect to mucosas such as oral cavity, throat, eye, stomaches, with document record have that numb feeling in the tongue is subjected to, clinical experience has the toxicity of " halberd people pharynx " to conform to, after finding the mouse peritoneal drug administration by injection in the experiment, all occur being slow in action, roll up, the high dose group of phenomenon, the especially needle of anorexia can see having that health trembles, Tachypneic performance.The LD50 value that can find out Rhizoma Typhonii fecula and its needle is respectively 2875 and 21.6mg/kg, and the toxicity of needle is more than 100 times of Rhizoma Typhonii life product.
Lead in the positive pill and adopted micronizing to prepare impalpable powder, eliminated Rhizoma Typhonii calcium oxalate crystal growth in healthy needle.Carrying out after the micronizing, microscopically is inspected needle-like calcium oxalate crystal, has proved conclusively not contain needle-like calcium oxalate crystal in the impalpable powder; Lead positive ball and eliminated the needle-like calcium oxalate crystal in the Rhizoma Typhonii, reduced its toxicity, guaranteed the safety of clinical application.
3, lead the dissolution rate that has improved effective ingredient after the positive ball micronizing
The TLC collection of illustrative plates shows, under same extracting method, lead the common fine powder of positive ball and in identical retention time identical absworption peak is arranged with superfine powder, just peak value has difference, illustrate that Chinese crude drug does not have novel substance to generate after micronizing, the variation of matter does not occur in the intrinsic pharmacodynamic basis material of Chinese medicine.
After supersound extraction, lead the stripping quantity of positive ball mesoxalic acid ammonium, superfine powder is higher by 73.44% than common flour respectively, illustrates after the micronizing that wherein the burst size of effective ingredient increases considerably.Effectively reduce dosage, thereby reduce cost, economize on resources.
Superfine powder is accelerated greatly than the dissolution rate of common flour, lead positive ball micronizing after, ammonium oxalate amount of stripping in 5min has substantially exceeded the maximum total amount of common flour stripping.Reason gives the credit to that grain size of micropowder is little, and the cell wall breaking rate is high, and stripping is not subjected to the obstruction of cell wall and cell membrane, so that the effective ingredient stripping quantity increases quickening.
4, lead the broken drug effect that improved of Scorpio superfine powder in the positive ball
Rat is divided into 7 groups at random, every group 20, be respectively Normal group, model control group, Scorpio superfine powder (1 μ m) group (2.5g crude drug/kg), Scorpio superfine powder (5 μ m) group (2.5g crude drug/kg), Scorpio superfine powder (10 μ m) group (2.5g crude drug/kg), the conventional powder of Scorpio (150 μ m) group (2.5g crude drug/kg) and positive drug nifedipine group (12mg/kg).
The continuous gastric infusion 10d of administration group, 1 time/d, capacity is the 1ml/lO0g body weight, Normal group and model control group give the equivalent distilled water.1h after the last administration, the anesthesia of lumbar injection 25% urethane, sublingual vein injection of pituitrin 0.5IU/kg/ (10s) brings out the coronary spasm myocardial ischemia, lead electrocardiogram with eight road physiology monitor record II, observe behind the injection hypophysis at once 15S, 30S, 1min, 2min, 5min electrocardiogram change, with T ripple, the change of ST section or P-R, Q-T interval prolongation judge degree of ischemia (criterion:
The 1st phase: namely be carved into 30s behind the injection of pituitrin, the T ripple raises, the ST section is raised above 0.1mv; The 2nd phase: the T ripple is low flat, two-phase, inversion, and heart rate is slack-off, P-R and Q-T interval prolongation).Experiment finishes common carotid artery and gets blood, and separation of serum is measured serum erythrocuprein (SOD), malonaldehyde (MDA) content, and serum lactate dehydrogenase (SLD) (LDH) is active, phosphokinase (CK) activity; Put to death rat, take out heart, make 10% homogenate with the ice normal saline, measure myocardium erythrocuprein (SOD), malonaldehyde (MDA) content.
The ischemic animal number average that Scorpio different-grain diameter superfine powder (1,5,10 μ m) group electrocardiogram occurs significantly is lower than model control group, shows that the myocardial ischemia that Scorpio different-grain diameter superfine powder causes pituitrin has obvious protection and improvement effect.Superfine powder 1,5, there was no significant difference between the 10 μ m particle diameters, testing result is as shown in table 2.
Table 2 different-grain diameter Scorpio superfine powder is on the Electrocardiographic impact of rats with myocardial ischemia
Annotate: compare with model control group: * P＜0.05, * * P＜0.01, n=20.
Model control group rat heart muscle SOD vigor compared with normal matched group reduces (P＜0.01), and the equal compared with normal control rats of MDA content reduces (P＜0.01); Scorpio different-grain diameter superfine powder (1,5,10m) group rat heart muscle SOD vigor is all than model control group rat rising (P＜0.01), cardiac muscle MDA content all reduces (P＜0.01) than the model control group rat, and effect all is better than conventional powder group, and (P＜0.O1), testing result is as shown in table 3.
Table 3 different-grain diameter Scolopendra superfine powder is to the rats with myocardial ischemia SOD of heart tissue, and the impact of MDA (x ± s)
Annotate: compare * P＜0.05, * * P＜0.01 with model control group; Compare with the conventional powder group of Scorpio,
△P＜0.05,△△P＜0.01；n=20
Model control group rat blood serum SOD vigor compared with normal matched group reduces (P＜0.01), and the equal compared with normal control rats of MDA content reduces (P＜0.01).Scorpio different-grain diameter superfine powder (1,5,10m) group rat SOD vigor is all than model control group rat rising (P＜0.01), MDA content all reduces (P＜0.01) than the model control group rat, and effect all is better than conventional powder group (P＜0.05), and testing result is as shown in table 4.
Table 4 different-grain diameter Scolopendra superfine powder is to the rats with myocardial ischemia SOD in serum, and the impact of MDA (x ± s)
Annotate: compare * P＜0.05, * * P＜0.01 with model control group; Compare with the conventional powder group of Scorpio,
△P＜0.05,△△P＜0.01；n=20。
Claims (10)
1. a preparation method of leading positive ball is characterized in that, may further comprise the steps:
1) in mass fraction, as crude drug, and crude drug cleaned remove impurity with the Scorpio of 3～5 parts Rhizoma Typhoniis, 3～5 parts Bombyx Batryticatus and 4～6 parts; Be lower than 10% with being dried to moisture mass fraction after the Scorpio fragmentation;
2) Scorpio after Rhizoma Typhonii, Bombyx Batryticatus and the fragmentation is mixed, after preliminary the pulverizing, adopt again super micron mill to be ground into impalpable powder, then sterilization;
3) impalpable powder with crude drug mixes with the mass ratio of yellow wine suspension by 3～10:1, the preparation soft material, and wherein the yellow wine suspension is that to be suspended with mass concentration in yellow wine be 3～10% cross-linking sodium carboxymethyl cellulose;
4) with soft material by moulding the method for making pill, coating after 50～80 ℃ of dryings; Dry again after coating is finished, obtain leading positive ball.
2. preparation method of leading positive ball as claimed in claim 1 is characterized in that, in mass fraction, with the Scorpio of 3.5～3.92 parts Rhizoma Typhoniis, 3.2～3.84 parts Bombyx Batryticatus and 4.27～5.0 parts as crude drug.
3. preparation method of leading positive ball as claimed in claim 1 is characterized in that, and is lower dry at 50～80 ℃ after the described Scorpio fragmentation.
4. preparation method of leading positive ball as claimed in claim 1 is characterized in that, does not contain the needle-like calcium oxalate crystal of Rhizoma Typhonii in the described impalpable powder.
5. preparation method of leading positive ball as claimed in claim 1 is characterized in that, cell wall breaking rate＞95% in the described impalpable powder.
6. preparation method of leading positive ball as claimed in claim 1 is characterized in that, the fineness of described impalpable powder is controlled to be can pass through 200 mesh sieves, sterilizes after crude drug was crossed 200 mesh sieves after super micron mill was pulverized.
7. preparation method of leading positive ball as claimed in claim 1 is characterized in that, described sterilization is to use cobalt
60Radiation sterilization.
8. preparation method of leading positive ball as claimed in claim 1 is characterized in that, the impalpable powder of crude drug is mixed with the mass ratio of yellow wine suspension by 5:1, and the mass concentration of cross-linking sodium carboxymethyl cellulose is 5% in the yellow wine suspension.
9. preparation method of leading positive ball as claimed in claim 1 is characterized in that, described coating is to carry out film coating to leading positive ball.
10. preparation method of leading positive ball as claimed in claim 1 is characterized in that, the described ball weight-normality lattice that lead positive ball are 0.1g.
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| CN201210493099.4A CN102949473B (en) | 2012-11-27 | 2012-11-27 | Preparation method of pullingqianzheng pills |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306919A (en) * | 2014-09-28 | 2015-01-28 | 郭长玉 | Traditional Chinese medicine for treating facial paralysis by external use |
| CN116135219A (en) * | 2023-04-07 | 2023-05-19 | 信阳师范学院 | Pinellia ternate attenuation treatment process |
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| CN1366947A (en) * | 2001-01-20 | 2002-09-04 | 杨孟君 | Nano Fufang Qianzheng preparation medicine and preparation method |
| CN102283959A (en) * | 2010-06-17 | 2011-12-21 | 苏州知微堂生物科技有限公司 | Preparation technology and production method of integrated novel Qianzheng powder dosage form |
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| CN1366947A (en) * | 2001-01-20 | 2002-09-04 | 杨孟君 | Nano Fufang Qianzheng preparation medicine and preparation method |
| CN102283959A (en) * | 2010-06-17 | 2011-12-21 | 苏州知微堂生物科技有限公司 | Preparation technology and production method of integrated novel Qianzheng powder dosage form |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306919A (en) * | 2014-09-28 | 2015-01-28 | 郭长玉 | Traditional Chinese medicine for treating facial paralysis by external use |
| CN116135219A (en) * | 2023-04-07 | 2023-05-19 | 信阳师范学院 | Pinellia ternate attenuation treatment process |
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| CN102949473B (en) | 2015-07-08 |
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