CN102925383B - Shewanella putrefaciens and application thereof in organic waste treatment - Google Patents
Shewanella putrefaciens and application thereof in organic waste treatment Download PDFInfo
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- CN102925383B CN102925383B CN201210383497.0A CN201210383497A CN102925383B CN 102925383 B CN102925383 B CN 102925383B CN 201210383497 A CN201210383497 A CN 201210383497A CN 102925383 B CN102925383 B CN 102925383B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a shewanella putrefaciens CR7 strain of which the preservation number is CGMCC NO.6192. The strain is separated out from cattle manure compost which is kept in a low-temperature environment for a long time; and a microbial inoculum prepared from the strain can be applied to livestock and poultry manure compost such as cattle manure and the like in winter, and has the effects of quickly starting compost fermentation, increasing compost fermentation temperature, shortening compost fermentation period and reducing nitrogen element loss. The microbial inoculum prepared from the strain is used for producing an organic fertilizer in winter, and can omit the heating procedure in the early stage of the composting of the organic fertilizer, shorten the production period of the organic fertilizer, increase the yield and lower the production cost.
Description
Technical field
The present invention relates to agricultural biological technical field, specifically relate to microorganism producing the technology application of biological organic fertilizer, is mainly a strain Shewanella putrefaciens and the application on organic waste treatment thereof.
Technical background
Extensively distribute and surviving a large amount of psychrophiles in exceeding the low temperature environments such as 80% ocean, polar region, glacier, high mountain and frozen soil accounting for earth surface
[they are in long-term evolutionary process, set up the growth mechanism that can adapt to cold environment, depend on unique low-temperature starting mover, even if its RNA polymerase also can be carried out efficiently transcribing of gene at 0 ℃, keep thalline normal growth and breeding at low temperatures, this vital movement phenomenon that is different from normal temperature bacterium and high temperature bacterium, its unique people's renewed attention that is worth.1975, Morita systematic account first psychrotropic bacteria, and its physilogical characteristics are analyzed, psychrophile can grow under cold environment, what people were familiar with have a liking for warm microorganism under cold environment performance make a living long stop even dead, reason be have a liking for warm microorganism at extreme environment enzyme activity too low and protein denaturation, show psychrophile and have a liking for warm microorganism and there is visibly different physiological mechanism.Psychrophile has potential using value in fields such as catalysis low temperature fermentation process and cold district sewage disposals.To the research of psychrophile resistance to cold, not only can disclose its acclimatization to cold mechanism, simultaneously for psychrophile application provides solid theoretical basis.In recent years, research to psychrophile acclimatization to cold mechanism and application thereof launches gradually, comprising having a liking for the basic structure feature of cold enzyme and the relation of its low temperature active, the molecular basis that the mobility of film system keeps and the component relationship of lipid acid, the fields such as cold shock protein and antifreeze protein structure and functional study.According to the standard of Morita, psychrophile is divided into two classes: can be 3 ~ 5 ℃ of growths, and maximum growth temperature is grown more than 20 ℃, and optimum growth temperature can be greater than the class of 15 ℃ and be called as psychrotrophs (psychrotoerants); Can be in the following growth of 0 ℃ or 0 ℃, optimum growth temperature is lower than 15 ℃, and maximum growth temperature is no more than the quasi-microorganism of 20 ℃ and is called as cryophile (psychrophiles).
The microbial spoilage Shewanella of the present invention application (
shewanella putrefaciens) belong to psychrotrophs.In the winter time under the low temperature environment of 0-10 ℃ the organic compost such as cow dung to play temperature be crucial, medium and high temperature bacterium is difficult to normal breeding at low temperatures, and psychrophile cytolemma contains a large amount of unsaturated fatty acidss, make film also can keep at low temperatures half flow state, transportation material; In addition Ribosome Structure and function are insensitive to low temperature, and enzyme and protein remains can synthesize and play a role.Therefore the present invention utilizes the special physiological mechanism of psychrophile, in compost under the low temperature environment of 0-10 ℃, add psychrophile in the winter time, at the compost fermentation initial stage, first these psychrophiles utilize a large amount of breeding fast of the simple organics such as the solubility sugar and starch in compost and produce heat, promote that compost temperature raises rapidly, temperature is elevated to 15-20 ℃ of amount reproduction that starts mesophilic microorganism, and prolific metabolism heat has guaranteed the warm process that rises of compost.The speed that compost opens warm process is to be determined by quantity and the reproduction speed of the psychrophile adding.The Shewanella putrefaciens of the present invention's application is exactly in the time of 0-10 ℃, in the organism such as cow dung, to breed the fastest microbial strains.The heat that the Fast-propagation of Shewanella putrefaciens produces can effectively promote the breeding of mesophilic bacteria and high temperature bacterium, and then has started the leavening temperature of compost, and compost can be reached a high temperature the stage fast, completes the harmless treatment of compost.
Microbe fermentation method is a kind of conventional low input, high benefit harmless treatment method for livestock manure, feces of livestock and poultry majority is natural packing fermentation, time is long, compost temperature is low, undesirable to killing with deodorizing effect of harmful microorganism, parasitic ovum, and long-term compost causes anaerobically fermenting to increase, anti-bacterial digestion is bred in a large number, a large amount of nitrogen element loss.Therefore, in the organism banking processes such as cow dung, add effective microbe, not only can accelerate the decomposition of organic carbon, reduce nitrogen loss, reduce the volatilization of ammonia, hydrogen sulfide in composting process, shorten fermentation time, and compost heats up high, can killing microorganism and parasite, ight soil is reached fully and become thoroughly decomposed, produce the green organic manure of high-quality.Waiting in the winter time under low temperature environment compost to play temperature is gordian technique, and can organism compost enter innoxious process and the fermentation period that the pliotherm period affects compost fast.In compost, the enzyme such as proteolytic enzyme and Mierocrystalline cellulose is lived and the highest microorganism of enzyme effect is mesophilic bacteria, once environment reaches the growth conditions of mesophilic bacteria, decomposing organic matter that just can be a large amount of, promotes heap temperature rise and high temperature bacterium amount reproduction, and generation biological heat enters the pliotherm period.Temperature is the important factor that compost carries out smoothly, can directly reflect compost process, and temperature rise is microbial metabolism heat production and the coefficient result of heap body insulation effect.
Report with disclosed technology in winter the warm method that opens that the production fertilizer such as cow dung adopts of fermenting be that the method for inputting hot steam and hot water in compost reaches the warm process of opening of compost, or directly lay the device of heating at the composting position of compost and control and increase the temperature of compost.To adopt heating unit at fermentation cabin bottom-heated such as the disclosed organism compost of patent CN10179234A opens warm method.Patent CN101200386B has invented one to heat and the adjustable spiral agitating type composting device of oxygenation, raises for the temperature of compost.These methods are all in earlier stage to start fermentation by increasing outside energy at organism compost, although method advanced person, applicable, the expensive cost with having increased greatly production fertilizer of comparison in equipment adopting.Only can in organic fertilizer production with good conditionsi, apply, and in middle-size and small-size organic fertilizer production enterprise, be difficult to promote.There is equally patent to disclose and utilize complex microorganism to be applied to low temperature organic matter fermentation, utilize the starter that subtilis, azotobacter chroococcum, viride are made to raise for the temperature of compost such as patent CN101381689A discloses; Patent CN101880182A adopts subtilis, bacillus megaterium, micromonospora and has a liking for cellulose-decomposing bacteria that to be used in low temperature be fermented livestock ight soil and Treating straw.The disclosed content of patent CN1800357A has been mentioned psychrotropic bacteria and thermophilic bacteria, but its psychrotropic bacteria of refering in particular to is fermented yeast and geotrichum candidum.The discloseder microorganisms of above-mentioned patent are that the common kind majority of occurring in nature belongs to mesophilic bacteria type.Although some bacterial classification adapts to low temperature environment, but concrete growing state and the quantity not openly explanation of these bacterial classifications under cold condition, the bacterial classification that these patents are mentioned does not comprise the Shewanella putrefaciens of this patent invention or the bacterial classification close with Shewanella putrefaciens.
Research and application about the Shewanella of low temperature environment also have many disclosed data, such as microorganism journal 2004,44(5): in 526-566, reported Shewanella under low temperature environment for degradation of dye.Number of patent application: 200910140943.3 and 201010220341.1 disclose Shewanella for microbiological fuel cell; 200310112361.7 disclose Shewanella for decolouring; 00806387.7 discloses Shewanella for the removing to petroleum hydrocarbon contaminant.These disclosed contents illustrate that Shewanella is a kind of microorganism of low temperature jointly, and the environmental energy that relatively adapts to low temperature is brought into play its function according to its feature.Applicant has consulted Shewanella that all disclosed data and document also do not find to adapt to low temperature environment and has opened application and the report aspect warm processing in organic waste for compost.
Summary of the invention
Open temperature slowly in order to solve the compost fermentation occurring in middle-size and small-size Organic Fertilizer Plants processing organic waste in winter low temperature situation, even cannot open warm technical barrier, (cow dung sample place in cryogenic refrigerator more than 6 months) is separated to prevailing microorganism strains in the efficacious microbial colony in the organism compost such as cow dung that a strain can be under low temperature environment, called after CR7 bacterial strain to the feature that the present invention makes full use of psychrophile from being positioned over for a long time the cow dung of low temperature.
CR7 bacterial strain provided by the present invention, through be accredited as Shewanella putrefaciens (
shewanella putrefaciens), deposit number is CGMCC No.6192.
The application of Shewanella putrefaciens CGMCC No.6192 of the present invention in organic waste treatment.
The invention also discloses a kind of microbiobacterial agent of being made by Shewanella putrefaciens CGMCC No.6192.
Described microbiobacterial agent can be prepared by following method:
(1) CR7 bacterium is activated under 5 ℃ of conditions of low temperature, select the fastest single bacterium colony of the speed of growth, as the bacterial classification of fermenting agent;
(2) according to formula (extractum carnis 3g, peptone 10g, sodium-chlor 5g, water 1L), the required substratum 2-4L of preparation fermentation, 121 ℃ of 20min sterilizings;
(3) bean powder and wheat bran are mixed evenly according to the volume ratio of 1:3, water content 30%, pack, every bag of 200g, wraps up 121 ℃ of 30min sterilizings, sterilizing twice altogether, interval 24h;
(4) bacterial classification of (1) activation is inoculated in the substratum of (2) preparing, puts 15 ℃ of 120r/min shaker fermentation 48h;
(5) under aseptic condition, by the bacterium liquid of step (3) fermentation material and step (4), according to volume ratio solid material: the ratio of bacterium liquid=2:5 mixes, and is placed on 30 ℃ of oven dry in baking oven, packs after sampling Detection.
(6) number of viable of CR7 in the sample that detecting step (5) is dried.The quantity of CR7 reaches 10
8above, conformance with standard.
The oven dry microbial inoculum of above-mentioned preparation, in packing bag, not under damaged condition, under room temperature condition, storage time is 6 months, and under 0-4 ℃ of condition, storage time is 2 years, and the quantity of preserving effective thalline is 10
5more than cfu/min.
above-mentioned microbial inoculum starts the application of fermentation under cold condition:
(1) expansion of microbial inoculum is numerous: puddle in organic material such as rice chaff or wheat bran according to the volume ratio of 1:10 at indoor (more than 10 ℃) microbial inoculum, add 30% warm water (20-30 ℃) of cumulative volume, stir, fermentation reactor system 10-20h.
(2) when the fresh cow dung of outdoor (0-10 ℃) composting is being added batching according to the method for producing fertilizer, add (1) to expand numerous startup microbial inoculum, the 1:1000 that add-on is volume ratio stirs, fermentation reactor system.
(3) in compost, put into thermometer, observe and add startup microbial inoculum and do not add the temperature variation in the compost that starts microbial inoculum (production of normal fertilizer).More than adding the treatment group temperature rise to 50 ℃ that starts microbial inoculum after compost 48h, exceed the temperature 10-15 ℃ of control group.Ferment successfully for starting.
Shewanella putrefaciens CGMCC No.6192 separates in the long-term cow dung compost in low temperature environment, and in the efficacious microbial colony in the organism compost such as cow dung, occupies an leading position under low temperature environment.The microbial inoculum being made by this bacterial classification is applied in the livestock excrement composting such as cow dung in the winter time, starts fast compost fermentation, improves compost fermentation temperature, shortens the fermentation period of compost, reduces the loss effect of nitrogen element.Improve output and reduce production cost.On the other hand, this microbial inoculum is applied in the short rotten agent that utilizes feces of livestock and poultry to produce fertilizer, no matter is all can guarantee the startup of organic fertilizer fermentation temperature and shorten the organic cycle of becoming thoroughly decomposed at indoor and outdoor production fertilizer in the winter time.
Embodiment
On June 6th, 2012, bacterial strain CR7 is delivered to the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, Classification And Nomenclature be Shewanella putrefaciens (
shewanella putrefaciens), deposit number is CGMCC No.6192; Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica; Postcode: 100101.
embodiment mono-: cow dung compost starts the separation of bacterial classification
The spice (adding 1% dregs of beans, l% rice chaff, 2% plant ash in cow dung) that gathers fresh cow dung and cow dung compost is positioned in 0-4 ℃ of refrigerator, after 6 months, adopt broth culture separate microorganism bacterial classification, separation method is isolation by dilution method, separation temperature is 1-4 ℃, in picking 48h, grow the microorganism strains of single bacterium colony, continue with broth culture inducing culture at low temperatures, be chosen in the microbial strains numbering that grows obvious single bacterium colony in 24h, save backup.
embodiment bis-: bacterial classification is adaptive mensuration in sterilizing cow dung culture medium
The serial bacterial classification that embodiment mono-sifts out is inoculated in the cow dung culture medium of sterilizing, and cultivating and filling a prescription is in cow dung, to add 1% dregs of beans, l% rice chaff, 2% plant ash.Set to 0 in the refrigerator of-5 ℃, survey thalline quantity every 2h, formulate the growth curve of each bacterial classification according to thalline quantity, be retained in growth in 48h and reach 10
5bacterial classification more than cfu/min, is chosen at breeding in the shortest time and arrives maximum growth quantity and the highest bacterial classification of thalline breeding quantity.Obtain by experiment a strain is the red bacterial classification of breast on extractum carnis substratum, and experiment numbers is CR7, and CR7 reaches 1.3 × 10 at 16h
9cfu/ml, OD
4001.473, than the intestinal bacteria CGMCC1.907 reproduction speed that reaches equivalent amount fast 4h.
embodiment tri-: CR7 bacterial strain is the existence quantitative measurement in composting cow dung in the open
The CR7 bacterial strain of purifying is cultivated 48 hours in beef-protein medium top fermentation, fermented liquid 4000r/min is centrifugal, retains throw out, i.e. CR7 bacterial strain thalline, these thalline are puddled according to the volume ratio of 1:10 in the rice chaff or wheat bran of sterilizing, measure thalline content 10
8more than cfu/min, and prepare CR7 microbial inoculum.Be inoculated in fresh cow dung according to 0.1% ratio, puddle evenly fermentation reactor system.Get the sample under 20cm in compost every day, in laboratory, adopt immediately the thalline quantity of CR7 bacterial strain in dilution separation method working sample.The substratum separating adopts beef-protein medium, the red bacteria colony count of the dull and stereotyped upper breast of investigation, the i.e. colony number of CR7 bacterial strain.48h reaches 1.7 × 10 after measured
9cfu/min.
embodiment tetra-: CR7 bacterial strain strain identification:
(1) physiology and morphology biochemical identification is with reference to the elegant pearl in east " common bacteria system identification handbook ", Science Press, calendar year 2001.
Through observing CR7 bacterial strain CGMCC No.6192: growth scope is 5-40 ℃, optimum growth temperature is 25 ℃ of left and right, growth pH scope 7.0-10.0.On beef extract-peptone solid medium, be newborn red bacterium colony, Gram-negative, the peroxidase positive, indoles, MR, VP and sugar-fermenting experiment are negative, produce H
2s.
(2) 16s rDNA identifies
1) design primer: P0:5 '-GAGAGTTTGATCCTGGCTCAG-3 ' (SEQ ID NO.1)
P5：5’-CTACGGCTACCTTGTTACGA-3’ （SEQ ID NO.2）
By Shanghai Sheng Gong company synthetic (P0 represents forward primer, and P5 represents reverse primer);
2) cultivation of bacterial strain is according to a conventional method: by CR7 inoculation in cultured solution of broth, 28 ℃, 180rpm
Under condition, shaking culture is to logarithmic phase;
3) amplification of bacterial strain 16SrDNA and sequential analysis
PCR reaction system: 10 × PCR buffer, 5 μ L, dNTP mixture (10mM) 1 μ L, MgCl
2(25mM) 4 μ L, primer P0 (5 μ M) 1 μ L, P5 (5 μ M) 1 μ L, Taq DNA polymerase (5U/ μ L) 0.5 μ L, Template 5 μ L, add ddH
2o causes final volume 50 μ L.
PCR reaction conditions: 95 ℃ of denaturation 10min, 94 ℃ of sex change 40s, 46 ℃ of annealing 40s, 72 ℃ are extended 1.5min, 30 circulations, 72 ℃ are cooling, and 4 ℃ to constant.
4) the rear PCR product of amplification is by Shanghai Sheng Gong Bioisystech Co., Ltd direct Sequencing.
The homology that sequencing result is CR7 and Shewanella putrefaciens at GENEBANK sequence comparative result reaches 99%, combining form and Physiology and biochemistry experimental result judge CR7 bacterial strain belong to Shewanella putrefaciens (
shewanella putrefaciens).
embodiment five:cR7
the preparation of microbial inoculum:
(1) CR7 bacterium is activated under 5 ℃ of conditions of low temperature, select the fastest single bacterium colony bacterial strain of the speed of growth, test tube preservation is as the bacterial strain of fermenting agent;
(2) according to formula (extractum carnis 3g, peptone 10g, sodium-chlor 5g, water 1L), preparation substratum 2-4L, 121 ℃ of 20min of sterilizing;
(3) by better than mixing according to the batch volume of 1:3 to bean powder and wheat bran, add the water of volume ratio 30%, every packed enter 200g spice, after bandaging, put into Autoclave, 121 ℃ of 30min of sterilizing, 121 ℃ of 30min sterilizings again after 24h, altogether sterilizing 2 times;
(4) bacterial classification of preservation is inoculated in fermention medium, 15 ℃, the 120r/min 48h that ferments;
(5) in Bechtop, first use 75% alcohol by porcelain dish sterilizing, again by after porcelain dish ultraviolet sterilization 20min, the fermentation material of sterilizing is laid on porcelain dish, the bacterium liquid that (4) are fermented, according to solid material: the ratio of bacterium liquid=200g:500mL, is poured on the spice of porcelain dish, stir with the glass stick of sterilizing, be placed on 30 ℃ of oven dry in baking oven;
(6) number of viable that adopts method of dilution butteron on plate to detect CR7 in (5) dry microbial inoculum reaches 10
8above, meet the standard (NY 227-94) of national fertilizer Inoculant.
embodiment six:cR7
the microbial inoculum startup fermentative action that cow dung compost is produced fertilizer in the winter time:
CR7 microbial inoculum will first expand numerous before application, expanding propagation method is: at indoor (more than 10 ℃), the dry microbial inoculum of packing is puddled according to the volume ratio of 1:10 in organic material such as rice chaff or wheat bran, 30% the warm water (20-30 ℃) that adds cumulative volume, stirs, fermentation reactor system 10-20h.In adding batching, add and expand numerous CR7 startup microbial inoculum according to the production method of producing fertilizer, the 1:1000 that add-on is volume ratio stirs, fermentation reactor system.
External temperature is 0-10 ℃ of left and right in the winter time, and the short rotten agent of the microorganism containing in the fresh cow dung of composting or the mesophilic bacteria adding is difficult to breeding, and microorganism does not breed and just not can produce temperature or microbial reproduction is very slow, and the heat of generation is little.
Added CR7 to start after microbial inoculum, CR7 bacterial classification will be bred very soon 5 ℃ of left and right.Its breeding meeting constantly produces heat, and the heat of generation will constantly promote microorganism around to start breeding, and in compost, a large amount of microorganisms all starts breeding and will inevitably produce a large amount of heats.Just very fast rising of the temperature of compost like this, generally adds more than CR7 starts microbial inoculum compost 48h temperature rise to 50 ℃, exceeds the compost temperature 10-15 ℃ that does not add CR7 to start microbial inoculum.It is to play the effect that starts and lead microorganism Fast-propagation in compost that CR7 starts adding of microbial inoculum.
embodiment seven:cR7
the effect that on cold-starting microbial inoculum and market, the short rotten agent of the cattle manure of public offering is used in conjunction with:
The microbial inoculum that adopts embodiment five to prepare, adds according to the method for embodiment six in the compost of cow dung, adds the short rotten agent (production of Hebi Ren Yuan bio-fertilizer Technology R & D Center) of the RW selling on market simultaneously, and product uses with reference to product description.Experimental site is selected in the rich chemical industry Organic Fertilizer Plants of Sihong County, Jiangsu Province paddy.
Experimental design:
Each treatment group is with 10 tons of designs of total amount, and fermentation material is respectively the pure cow dung 6-7 ton that humidity is 65% left and right, rice chaff 1-2 ton, 1 ton of plant ash, treatment group one: the short rotten agent of CR7 solid list microbial inoculum and RW mixes according to the 1:10 of volume ratio, and every 10 tons add 2kg; Treatment group two: every 10 tons add 2kgRW to urge rotten agent; Control group does not add any short rotten agent.The fertilizer heap of every group is designed to long × wide × high heap body for 12m × 1.5m × 1.2m, turns over throwing with turnover throwing machine, stirs, and the fermentation of bar buttress formula.9 of every mornings and afternoons 4 point, first measure external temperature, then insert under fertile heap 30-40cm with thermometer, keep 10min, read the temperature of fertile heap, record numerical value.
Sample at the starting and ending of compost fermentation respectively.With in soil sample barrel insertion heap, more than every heap is got 5 points.The sample of obtaining mixes immediately, with the freezing preservation of sealing bag.3 parallel sample of the each collection of every processing.Dry immediately, measure the variation of full nitrogen and full carbon with the fertilizer new standard NY525-2011 of the People's Republic of China (PRC), calculate C/N and change and change of soil water content, analyze compost and whether become thoroughly decomposed.
Experimental result:
Record every day outdoor medial temperature 5 ℃ of left and right, treatment group one, the compost time is in 48h left and right, temperature rise to 30 ℃ left and right, in 72h, fertile stack temperature is raised to more than 50 ℃; Treatment group two, the compost time, in 60h temperature rise to 30 ℃, reaches 50 ℃ of needs 80h; The fertile stack temperature of control group does not change substantially.Repeat to experimental results show that result is the same for twice, the RW microbial inoculum that adds the mono-microbial inoculum of CR7 to mix, fertile heap starts fermentation time and shortens 20h.Rise to 2.01% according to fertilizer new standard NY525-2011 mensuration total nitrogen content by 1.78% and be reduced to 31.32% with full carbon by 38.02%, C/N is reduced to 15.58 by 21.36, according to document (Wu Yinbao, Wang Zhisan. the research of compost maturity index. agro-environment science journal, 2003,22 (2): 189-193.) C/N is less than 20, then reaches 50 ℃ in conjunction with compost temperature and have above 4-5d, thinks that compost reaches to become thoroughly decomposed.Therefore the RW microbial inoculum that uses the mono-microbial inoculum of CR7 to mix can meet the requirement of compost, and can, under 5 ℃ of conditions of low temperature, start fermentation, shortens fermentation time 20-30d left and right.
SEQUENCE LISTING
<110> Nanjing Normal University
<120> mono-strain Shewanella putrefaciens and the application on organic waste treatment thereof
<130>
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 21
<212> DNA
<213> artificial sequence
<400> 1
gagagtttga tcctggctca g 21
<210> 2
<211> 20
<212> DNA
<213> artificial sequence
<400> 2
ctacggctac cttgttacga 20
Claims (3)
- One strain Shewanella putrefaciens ( shewanella putrefaciens) CR7 bacterial strain, its preserving number is CGMCC No.6192.
- 2. the application of Shewanella putrefaciens CGMCC No.6192 in livestock excrement composting fermentation.
- 3. Shewanella putrefaciens CGMCC No.6192 utilizes feces of livestock and poultry to produce the application in the short rotten agent of fertilizer in preparation.
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| CN103509744B (en) * | 2013-09-11 | 2015-07-15 | 上海交通大学 | Algicidal Shewanella and application thereof in controlling blue algae water bloom |
| CN103820544B (en) * | 2014-01-28 | 2016-01-06 | 浙江工商大学 | A kind of separation and detection method of Shewanella putrefaciens in fishery products |
| CN105296398A (en) * | 2015-11-12 | 2016-02-03 | 天津大学 | Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof |
| CN109593686B (en) * | 2019-01-18 | 2021-05-25 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Shewanella and application thereof in controlling nitrogen pollution of vegetable field soil |
| CN112342150B (en) * | 2019-08-06 | 2022-08-30 | 中国石油化工股份有限公司 | Petroleum degrading bacterium and application thereof |
| CN110656064A (en) * | 2019-10-24 | 2020-01-07 | 苏州市双利环保工程有限公司 | Shewanella putrefaciens and culture method and application thereof |
| CN111302854B (en) * | 2020-02-16 | 2021-11-02 | 山东新富瑞农业科技有限公司 | Biological fertilizer and preparation method thereof |
| CN115125166B (en) * | 2022-06-08 | 2023-06-20 | 北京林业大学 | Method for enhancing anaerobic treatment of kitchen waste by using conductive carrier to form Shewanella electroactive biomembrane |
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| 秦蕾 等.一种新的异育银鲫病原——腐败希瓦氏菌.《微生物学报》.2012,第52卷(第5期),第558-565页. |
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