CN102925383A - Shewanella putrefaciens and application thereof in organic waste treatment - Google Patents
Shewanella putrefaciens and application thereof in organic waste treatment Download PDFInfo
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- CN102925383A CN102925383A CN2012103834970A CN201210383497A CN102925383A CN 102925383 A CN102925383 A CN 102925383A CN 2012103834970 A CN2012103834970 A CN 2012103834970A CN 201210383497 A CN201210383497 A CN 201210383497A CN 102925383 A CN102925383 A CN 102925383A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a shewanella putrefaciens CR7 strain of which the preservation number is CGMCC NO.6192. The strain is separated out from cattle manure compost which is kept in a low-temperature environment for a long time; and a microbial inoculum prepared from the strain can be applied to livestock and poultry manure compost such as cattle manure and the like in winter, and has the effects of quickly starting compost fermentation, increasing compost fermentation temperature, shortening compost fermentation period and reducing nitrogen element loss. The microbial inoculum prepared from the strain is used for producing an organic fertilizer in winter, and can omit the heating procedure in the early stage of the composting of the organic fertilizer, shorten the production period of the organic fertilizer, increase the yield and lower the production cost.
Description
Technical field
The present invention relates to agricultural biological technical field, specifically relate to microorganism and use in the technology of producing biological organic fertilizer, mainly is a strain Shewanella putrefaciens and the application on organic waste treatment thereof.
Technical background
Taking up an area the ball surface above extensively distributing in 80% the low temperature environments such as ocean, polar region, glacier, high mountain and frozen soil and surviving a large amount of psychrophiles
[They are in long-term evolutionary process, set up the growth mechanism that can adapt to cold environment, depend on unique low-temperature starting mover, even its RNA polymerase also can be carried out efficiently transcribing of gene at 0 ℃, keep thalline normal growth and breeding at low temperatures, this vital movement phenomenon that is different from normal temperature bacterium and high temperature bacterium, its unique people's renewed attention that is worth.1975, first systematic account of Morita psychrotropic bacteria, and its physilogical characteristics have been analyzed, psychrophile can grow under cold environment, the warm microorganism of having a liking for that people were familiar with then shows long the stopping even death of making a living under cold environment, reason is to have a liking for warm microorganism to cross low and protein denaturation at the extreme environment enzyme activity, shows psychrophile and have a liking for warm microorganism to have visibly different physiological mechanism.Psychrophile has potential using value in fields such as catalysis low temperature fermentation process and cold district sewage disposals.To the research of psychrophile resistance to cold, not only can disclose its acclimatization to cold mechanism, using for psychrophile simultaneously provides solid theoretical basis.In recent years, research to psychrophile acclimatization to cold mechanism and application thereof launches gradually, comprising the basic structure characteristics of having a liking for cold enzyme and the relation of its low temperature active, the molecular basis that the flowability of film system keeps and the component relationship of lipid acid, the fields such as cold shock protein and antifreeze protein structure and functional study.According to the standard of Morita, psychrophile is divided into two classes: can be 3 ~ 5 ℃ of growths, and maximum growth temperature is in growth more than 20 ℃, and optimum growth temperature can be called as greater than a class of 15 ℃ psychrotrophs (psychrotoerants); Can be in growth below 0 ℃ or 0 ℃, optimum growth temperature is lower than 15 ℃, and maximum growth temperature is no more than a quasi-microorganism of 20 ℃ and is called as cryophile (psychrophiles).
The microbial spoilage Shewanella that the present invention uses (
Shewanella putrefaciens) belong to psychrotrophs.In the winter time under 0-10 ℃ the low temperature environment the organic compost such as cow dung to play temperature be crucial, the medium and high temperature bacterium is difficult to normal breeding at low temperatures, and the psychrophile cytolemma contains a large amount of unsaturated fatty acidss, makes film also can keep at low temperatures half mobile state, transportation material; Ribosome Structure and function are insensitive to low temperature in addition, and enzyme and protein remains can synthesize and play a role.Therefore the present invention utilizes the special physiological mechanism of psychrophile, add psychrophile in the compost under 0-10 ℃ the low temperature environment in the winter time, at the compost fermentation initial stage, these psychrophiles at first utilize a large amount of fast breeding of the simple organics such as solubility sugar and starch in the compost and produce heat, promote that compost temperature raises rapidly, temperature is elevated to 15-20 ℃ of amount reproduction that starts mesophilic microorganism, and prolific metabolism heat has guaranteed the warm process that rises of compost.The speed that compost opens warm process is to be determined by the quantity of the psychrophile that adds and reproduction speed.The Shewanella putrefaciens that the present invention uses is exactly to breed the fastest microbial strains in the time of 0-10 ℃ in the organism such as cow dung.The heat that the Fast-propagation of Shewanella putrefaciens produces can effectively promote the breeding of mesophilic bacteria and high temperature bacterium, and then has started the leavening temperature of compost, and compost can be reached a high temperature the stage fast, finishes the harmless treatment of compost.
Microbe fermentation method is a kind of low input commonly used, high benefit harmless treatment method for livestock manure, the feces of livestock and poultry majority is the natural packing fermentation, time is long, compost temperature is low, undesirable to killing with deodorizing effect of harmful microorganism, parasitic ovum, and long-term compost causes anaerobically fermenting to increase, and anti-bacterial digestion is bred in a large number, a large amount of nitrogen element loss.Therefore, in the organism banking processes such as cow dung, add effective microbe, not only can accelerate core sediments, reduce nitrogen loss, reduce the volatilization of ammonia, hydrogen sulfide in the composting process, shorten fermentation time, and compost heats up high, can killing microorganism and parasite, ight soil is reached fully become thoroughly decomposed, produce the green organic manure of high-quality.Waiting in the winter time under the low temperature environment compost to play temperature is gordian technique, and can the organism compost enter innoxious process and the fermentation period that the pliotherm period affects compost fast.The enzyme such as proteolytic enzyme and Mierocrystalline cellulose is lived and the highest microorganism of enzyme effect is mesophilic bacteria in compost, in case environment reaches the growth conditions of mesophilic bacteria, decomposing organic matter that just can be a large amount of promotes heap temperature rise and high temperature bacterium amount reproduction, and the generation biological heat enters the pliotherm period.Temperature is the important factor that compost carries out smoothly, can directly reflect the compost process, and it is microbial metabolism heat production and the coefficient result of heap body insulation effect that temperature rises.
Report with disclosed technology in winter the warm method that opens of fermenting that the production fertilizer such as cow dung adopts be that the method for input hot steam and hot water reaches the warm process of opening of compost in compost, perhaps directly lay the temperature that compost was controlled and increased to the device of heating at the composting position of compost.The method that opens temperature such as the disclosed organism compost of patent CN10179234A is to adopt heating unit at the fermentation cabin bottom-heated.Patent CN101200386B has invented an adjustable spiral agitating type composting device that heats with oxygenation, and the temperature that is used for compost raises.These methods all are in earlier stage to start fermentation by the energy that increases the outside at the organism compost, although method is advanced, be suitable for the expensive cost with having increased greatly the production fertilizer of the comparison in equipment that adopts.Only can in organic fertilizer production with good conditionsi, use, and in middle-size and small-size organic fertilizer production enterprise, be difficult to promote.Equally patent disclosure has been arranged and utilized complex microorganism to be applied to the low temperature organic matter fermentation, disclose the temperature rising that the starter that utilizes subtilis, azotobacter chroococcum, viride to make is used for compost such as patent CN101381689A; Patent CN101880182A adopts subtilis, bacillus megaterium, micromonospora and has a liking for cellulose-decomposing bacteria that to be used in low temperature be fermented livestock ight soil and Treating straw.The disclosed content of patent CN1800357A has been mentioned psychrotropic bacteria and thermophilic bacteria, but its psychrotropic bacteria of refering in particular to is fermented yeast and geotrichum candidum.Some microorganisms of above-mentioned patent disclosure are that the common kind majority of occurring in nature belongs to the mesophilic bacteria type.Although some bacterial classification adapts to low temperature environment, but concrete growing state and the quantity not openly explanation of these bacterial classifications under cold condition, the bacterial classification that these patents are mentioned does not comprise the Shewanella putrefaciens of this patent invention or the bacterial classification close with Shewanella putrefaciens.
About the research of the Shewanella of low temperature environment with use many disclosed data are also arranged, such as microorganism journal 2004,44(5): reported among the 526-566 that Shewanella is used for degradation of dye under low temperature environment.Number of patent application: 200910140943.3 and 201010220341.1 disclose Shewanella is used for microbiological fuel cell; Be used for decolouring 200310112361.7 disclose Shewanella; 00806387.7 disclose Shewanella for the removing to petroleum hydrocarbon contaminant.These disclosed contents illustrate that jointly Shewanella is a kind of microorganism of low temperature, and the environmental energy that relatively adapts to low temperature is brought into play its function according to its characteristics.The applicant has consulted all disclosed data and document and has also found to adapt to the Shewanella of low temperature environment and be used for compost open application and report aspect warm in processing organic waste.
Summary of the invention
Open temperature slowly in order to solve the compost fermentation that occurs in the middle-size and small-size Organic Fertilizer Plants processing organic waste in the winter low temperature situation, even can't open warm technical barrier, the characteristics that the present invention takes full advantage of psychrophile (cow dung sample place in the cryogenic refrigerator more than 6 months) from the cow dung that is positioned over for a long time low temperature be separated to a strain can the efficacious microbial colony in the organism compost such as cow dung under the low temperature environment in prevailing microorganism strains, called after CR7 bacterial strain.
CR7 bacterial strain provided by the present invention, through be accredited as Shewanella putrefaciens (
Shewanella putrefaciens), deposit number is CGMCC No.6192.
The application of Shewanella putrefaciens CGMCC No.6192 of the present invention in organic waste treatment.
The invention also discloses a kind of microbiobacterial agent of being made by Shewanella putrefaciens CGMCC No.6192.
Described microbiobacterial agent can prepare by following method:
(1) the CR7 bacterium is activated under 5 ℃ of conditions of low temperature, select the fastest single bacterium colony of the speed of growth, as the bacterial classification of fermenting agent;
(2) according to prescription (extractum carnis 3g, peptone 10g, sodium-chlor 5g, water 1L), the required substratum 2-4L of preparation fermentation, 121 ℃ of 20min sterilizations;
(3) bean powder and wheat bran are mixed evenly according to the volume ratio of 1:3, water content 30%, pack, every bag of 200g wraps up 121 ℃ of 30min sterilizations, sterilizes altogether twice, interval 24h;
(4) bacterial classification inoculation with (1) activation arrives in the substratum of (2) preparation, puts 15 ℃ of 120r/min shaker fermentation 48h;
(5) under aseptic condition, with the bacterium liquid of step (3) fermentation material and step (4), according to volume ratio solid material: the ratio of bacterium liquid=2:5 mixes, and is placed on 30 ℃ of oven dry in the baking oven, packs after the sampling Detection.
(6) number of viable of CR7 in the sample of detecting step (5) oven dry.The quantity of CR7 reaches 10
8More than, conformance with standard.
The oven dry microbial inoculum of above-mentioned preparation, not under the damaged condition, storage time is 6 months under the room temperature condition in packing bag, and storage time is 2 years under the 0-4 ℃ of condition, and the quantity of preserving effective thalline is 10
5More than the cfu/min.
Above-mentioned microbial inoculum starts the application of fermentation under cold condition:
(1) expansion of microbial inoculum is numerous: puddle in organic material such as rice chaff or wheat bran according to the volume ratio of 1:10 at indoor (more than 10 ℃) microbial inoculum, add 30% warm water (20-30 ℃) of cumulative volume, stir fermentation reactor system 10-20h.
(2) add (1) when the fresh cow dung of outdoor (0-10 ℃) composting is being added batching according to the method for producing fertilizer and expand numerous startup microbial inoculum, add-on is that the 1:1000 of volume ratio stirs fermentation reactor system.
(3) put into thermometer in the compost, observe to add start microbial inoculum and do not add temperature variation in the compost that starts microbial inoculum (the normally production of fertilizer).Add the treatment group temperature that starts microbial inoculum behind the compost 48h and rise to more than 50 ℃, exceed temperature 10-15 ℃ of control group.Ferment successfully for starting.
Shewanella putrefaciens CGMCC No.6192 separation is in the cow dung compost of low temperature environment certainly for a long time, and occupies an leading position in the efficacious microbial colony in the organism compost such as cow dung under the low temperature environment.The microbial inoculum that is made by this bacterial classification is used in the livestock excrement composting such as cow dung in the winter time, starts fast compost fermentation, improves the compost fermentation temperature, shortens the fermentation period of compost, reduces the loss effect of nitrogen element.Improve output and Decrease production cost.On the other hand, in the short rotten agent that this microbial inoculum is applied to utilize feces of livestock and poultry to produce fertilizer, no matter be all can guarantee the startup of organic fertilizer fermentation temperature and shorten the organic cycle of becoming thoroughly decomposed at indoor and open-air production fertilizer in the winter time.
Embodiment
On June 6th, 2012 bacterial strain CR7 is delivered China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, Classification And Nomenclature be Shewanella putrefaciens (
Shewanella putrefaciens), deposit number is CGMCC No.6192; Depositary institution address: No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Postcode: 100101.
Embodiment one: cow dung compost starts the separation of bacterial classification
The spice (adding 1% dregs of beans, l% rice chaff, 2% plant ash in the cow dung) that gathers fresh cow dung and cow dung compost is positioned in the 0-4 ℃ of refrigerator, adopt broth culture separate microorganism bacterial classification after 6 months, separation method is isolation by dilution method, separation temperature is 1-4 ℃, grow the microorganism strains of single bacterium colony in the picking 48h, continuation is chosen in the microbial strains numbering that grows obvious single bacterium colony in the 24h with broth culture inducing culture at low temperatures, saves backup.
Embodiment two: bacterial classification is adaptive mensuration in the sterilization cow dung culture medium
The serial bacterial classification inoculation that embodiment one sifts out is in the cow dung culture medium of sterilization, and cultivating and filling a prescription is to add 1% dregs of beans, l% rice chaff, 2% plant ash in the cow dung.Set to 0 in-5 ℃ the refrigerator, survey thalline quantity every 2h, formulate the growth curve of each bacterial classification according to thalline quantity, being retained in that growth reaches 10 in the 48h
5The bacterial classification that cfu/min is above is chosen at breeding arrival maximum growth quantity and the highest bacterial classification of thalline breeding quantity in the shortest time.Obtaining by experiment a strain is the red bacterial classification of breast at the extractum carnis substratum, and experiment numbers is CR7, and CR7 reaches 1.3 * 10 at 16h
9Cfu/ml, OD
4001.473, than the intestinal bacteria CGMCC1.907 reproduction speed that reaches equivalent amount fast 4h.
Embodiment three: the CR7 bacterial strain is the existence quantitative measurement in the composting cow dung in the open
The CR7 bacterial strain of purifying was cultivated 48 hours in the beef-protein medium top fermentation, fermented liquid 4000r/min is centrifugal, keeps throw out, i.e. CR7 bacterial strain thalline, these thalline are puddled in the rice chaff or wheat bran of sterilization according to the volume ratio of 1:10, measure thalline content 10
8More than the cfu/min, and prepared the CR7 microbial inoculum.Ratio according to 0.1% is inoculated in the fresh cow dung, puddles evenly fermentation reactor system.Get the sample under the 20cm in the compost every day, in the laboratory, adopt immediately the thalline quantity of CR7 bacterial strain in the dilution separation method working sample.The substratum that separates adopts beef-protein medium, the red bacteria colony count of the dull and stereotyped upper breast of investigation, the i.e. colony number of CR7 bacterial strain.48h reaches 1.7 * 10 after measured
9Cfu/min.
Embodiment four: CR7 bacterial strain strain identification:
(1) the physiology and morphology biochemical identification is with reference to the elegant pearl in east " common bacteria system identification handbook, Science Press, calendar year 2001.
Through observing CR7 bacterial strain CGMCC No.6192: growth scope is 5-40 ℃, optimum growth temperature about 25 ℃, growth pH scope 7.0-10.0.Newborn red bacterium colony at the beef extract-peptone solid medium, Gram-negative, peroxidase is positive, and indoles, MR, VP and sugar-fermenting experiment are negative, produce H
2S.
(2) 16s rDNA identifies
1) design primer: P0:5 '-GAGAGTTTGATCCTGGCTCAG-3 ' (SEQ ID NO.1)
P5：5’-CTACGGCTACCTTGTTACGA-3’ （SEQ ID NO.2）
Give birth to worker company synthetic (P0 represents forward primer, and P5 represents reverse primer) by Shanghai;
2) cultivation of bacterial strain is according to a conventional method: with the CR7 inoculation in cultured solution of broth, 28 ℃, 180rpm
Shaking culture is to logarithmic phase under the condition;
3) amplification of bacterial strain 16SrDNA and sequential analysis
PCR reaction system: 10 * PCR buffer, 5 μ L, dNTP mixture (10mM) 1 μ L, MgCl
2(25mM) 4 μ L, primer P0 (5 μ M) 1 μ L, P5 (5 μ M) 1 μ L, Taq DNA polymerase (5U/ μ L) 0.5 μ L, Template 5 μ L add ddH
2O causes final volume 50 μ L.
The PCR reaction conditions: 95 ℃ of denaturation 10min, 94 ℃ of sex change 40s, 46 ℃ of annealing 40s, 72 ℃ are extended 1.5min, 30 circulations, 72 ℃ of coolings, 4 ℃ to constant.
4) the PCR product is given birth to worker Bioisystech Co., Ltd direct Sequencing by Shanghai after the amplification.
Sequencing result is that the homology of CR7 and Shewanella putrefaciens reaches 99% at GENEBANK sequence comparative result, combining form and Physiology and biochemistry experimental result judge the CR7 bacterial strain belong to Shewanella putrefaciens (
Shewanella putrefaciens).
Embodiment five:CR7
The preparation of microbial inoculum:
(1) the CR7 bacterium is activated under 5 ℃ of conditions of low temperature, select the fastest single bacterium colony bacterial strain of the speed of growth, the test tube preservation is as the bacterial strain of fermenting agent;
(2) according to prescription (extractum carnis 3g, peptone 10g, sodium-chlor 5g, water 1L), preparation substratum 2-4L, 121 ℃ of 20min sterilize;
(3) bean powder and wheat bran is better than mixing according to the batch volume of 1:3, add the water of volume ratio 30%, every packed 200g spice that enters after bandaging, is put into Autoclave, the 121 ℃ of 30min that sterilizes, behind the 24h again 121 ℃ of 30min sterilize, sterilize altogether 2 times;
(4) with the bacterial classification inoculation of preservation in fermention medium, 15 ℃, the 120r/min 48h that ferments;
(5) in Bechtop, with 75% alcohol porcelain dish is sterilized first, again with behind the porcelain dish ultraviolet sterilization 20min, the fermentation material of sterilization is tiled on the porcelain dish, the bacterium liquid that (4) are fermented, according to the solid material: the ratio of bacterium liquid=200g:500mL is poured on the spice of porcelain dish, glass stick with sterilization stirs, and is placed on 30 ℃ of oven dry in the baking oven;
(6) number of viable that adopts method of dilution butteron on plate to detect CR7 in (5) dry microbial inoculum reaches 10
8More than, meet the standard (NY 227-94) of national fertilizer Inoculant.
Embodiment six:CR7
Microbial inoculum is the startup fermentative action of cow dung compost production fertilizer in the winter time:
The CR7 microbial inoculum will expand first numerous before application, expanding propagation method is: will pack dry microbial inoculum at indoor (more than 10 ℃) and puddle in organic material such as rice chaff or wheat bran according to the volume ratio of 1:10, add 30% warm water (20-30 ℃) of cumulative volume, stir fermentation reactor system 10-20h.Add the numerous CR7 of expansion according to the production method of producing fertilizer when adding batching and start microbial inoculum, add-on is that the 1:1000 of volume ratio stirs fermentation reactor system.
External temperature is about 0-10 ℃ in the winter time, and the short rotten agent of the microorganism that contains in the fresh cow dung of composting or the mesophilic bacteria of adding is difficult to breeding, and microorganism does not breed and just not can produce temperature or microbial reproduction is very slow, and the heat of generation seldom.
After having added CR7 startup microbial inoculum, the CR7 bacterial classification will very fast breeding about 5 ℃.Its breeding meeting constantly produces heat, and the microorganism of the heat of generation around will constantly promoting begins breeding, and a large amount of microorganisms all begins to breed and will inevitably produce a large amount of heats in the compost.So just very fast rising of the temperature of compost generally adds CR7 startup microbial inoculum compost 48h temperature and rises to more than 50 ℃, exceeds compost temperature 10-15 ℃ that does not add CR7 startup microbial inoculum.The adding that CR7 starts microbial inoculum is to play the effect that starts and lead microorganism Fast-propagation in the compost.
Embodiment seven:CR7
The effect that the short rotten agent of the cattle manure of public offering is used on cold-starting microbial inoculum and the market:
Adopt the microbial inoculum of embodiment five preparations, add in the compost of cow dung according to the method for embodiment six, add simultaneously the short rotten agent of the RW that sells on the market (people from Hebi unit bio-fertilizer technical research center produces), product uses with reference to product description.Experimental site is selected in the rich chemical industry Organic Fertilizer Plants of Sihong County, Jiangsu Province paddy.
Experimental design:
Each treatment group is with 10 tons of designs of total amount, and it is about 65% pure cow dung 6-7 ton that fermentation material is respectively humidity, rice chaff 1-2 ton, and 1 ton of plant ash, treatment group one: the short rotten agent of CR7 solid list microbial inoculum and RW mixes according to the 1:10 of volume ratio, and per 10 tons add 2kg; Treatment group two: per 10 tons add the short rotten agent of 2kgRW; Control group does not add any short rotten agent.Every group fertilizer heap is designed to long * wide * height for the heap body of 12m * 1.5m * 1.2m, turns over throwing with turnover throwing machine, stirs the fermentation of bar buttress formula.9 of every mornings and afternoons 4 point, measure first external temperature, insert under the fertile heap 30-40cm with thermometer again, keep 10min, read the temperature of fertile heap, record numerical value.
Take a sample at the starting and ending of compost fermentation respectively.Insert in the heap with soil sample barrel, every heap is got more than 5 points.The sample of obtaining mixes immediately, with the freezing preservation of sealing bag.Every processing gathers 3 parallel sample.Whether immediately oven dry is measured full nitrogen and the full variation of carbon with the fertilizer new standard NY525-2011 of the People's Republic of China (PRC), calculates C/N and changes and change of soil water content, analyze compost and become thoroughly decomposed.
Experimental result:
Record every day outdoor medial temperature about 5 ℃, treatment group one, the compost time, temperature rose to about 30 ℃ about 48h, and fertile stack temperature is raised to more than 50 ℃ in the 72h; Treatment group two, the compost time rises to 30 ℃ in the 60h temperature, reaches 50 ℃ of needs 80h; The fertile stack temperature of control group does not have to change substantially.Repeat twice and experimental results show that the result is the same, add the RW microbial inoculum that the single microbial inoculum of CR7 mixes, fertile heap starts fermentation time and shortens 20h.According to fertilizer new standard NY525-2011 measure total nitrogen content by 1.78% rise to 2.01% and full carbon be reduced to 31.32% by 38.02%, C/N is reduced to 15.58 by 21.36, according to document (Wu Yinbao, Wang Zhisan. the research of compost maturity index. the agro-environment science journal, 2003,22 (2): 189-193.) C/N is less than 20, and reaching more than 50 ℃ in conjunction with compost temperature has 4-5d again, thinks that compost reaches to become thoroughly decomposed.Therefore the RW microbial inoculum that uses the single microbial inoculum of CR7 to mix can satisfy the requirement of compost, and can start fermentation under 5 ℃ of conditions of low temperature, shortens about fermentation time 20-30d.
SEQUENCE LISTING
＜110〉Nanjing Normal University
＜120〉a strain Shewanella putrefaciens and the application on organic waste treatment thereof
<130>
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 21
<212> DNA
＜213〉artificial sequence
<400> 1
gagagtttga tcctggctca g 21
<210> 2
<211> 20
<212> DNA
＜213〉artificial sequence
<400> 2
ctacggctac cttgttacga 20
Claims (5)
- One strain Shewanella putrefaciens ( Shewanella putrefacienS) CR7 bacterial strain, its preserving number are CGMCC No.6192.
- 2. the application of Shewanella putrefaciens CGMCC No.6192 in processing organic waste.
- 3. the application of Shewanella putrefaciens CGMCC No.6192 in the livestock excrement composting fermentation.
- 4. Shewanella putrefaciens CGMCC No.6192 utilizes feces of livestock and poultry to produce application in the short rotten agent of fertilizer in preparation.
- 5. a Shewanella putrefaciens CGMCC No.6192 microbiobacterial agent is characterized in that Shewanella putrefaciens CGMCC No.6192 number of viable reaches 10 8More than.
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Cited By (8)
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| CN103509744A (en) * | 2013-09-11 | 2014-01-15 | 上海交通大学 | Algicidal Shewanella and application thereof in controlling blue algae water bloom |
| CN103820544A (en) * | 2014-01-28 | 2014-05-28 | 浙江工商大学 | Separation and detection method for shewanella putrefaciens in aquatic products |
| CN105296398A (en) * | 2015-11-12 | 2016-02-03 | 天津大学 | Low-temperature-resistant composite microbial agent used for treating contaminated water, and preparation method and application thereof |
| CN109593686A (en) * | 2019-01-18 | 2019-04-09 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | A kind of Shewanella and its application in control vegetable field soil nitrogen pollution |
| CN110656064A (en) * | 2019-10-24 | 2020-01-07 | 苏州市双利环保工程有限公司 | Shewanella putrefaciens and culture method and application thereof |
| CN111302854A (en) * | 2020-02-16 | 2020-06-19 | 甘肃省农业科学院旱地农业研究所 | Biological fertilizer and preparation method thereof |
| CN112342150A (en) * | 2019-08-06 | 2021-02-09 | 中国石油化工股份有限公司 | Petroleum degrading bacterium and application thereof |
| CN115125166A (en) * | 2022-06-08 | 2022-09-30 | 北京林业大学 | Method for strengthening anaerobic treatment of kitchen waste by forming Shewanella electroactive biomembrane by using conductive carrier |
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| CN109593686B (en) * | 2019-01-18 | 2021-05-25 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Shewanella and application thereof in controlling nitrogen pollution of vegetable field soil |
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