CN102920920B - Efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation - Google Patents
Efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation Download PDFInfo
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- CN102920920B CN102920920B CN201210382677.7A CN201210382677A CN102920920B CN 102920920 B CN102920920 B CN 102920920B CN 201210382677 A CN201210382677 A CN 201210382677A CN 102920920 B CN102920920 B CN 102920920B
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Abstract
The invention discloses an efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation which is characterized by consisting of the following raw materials in parts by weight: 35-40 parts of rhizoma gastrodiae, 37-43 parts of cortex eucommiae, 5-7 parts of angelica, 5-7 parts of radix scrophulariae, 5-7 parts of rhizoma et radix notopterygii, 2-4 parts of radix angelicae pubescentis, 0.5-1 part of radix aconiti agrestis, 0.5-1 part of radix aconiti lateralis preparata, 4-6 parts of mistletoe, 10-13 parts of semen citri reticulatae, 8-12 parts of raddle and 8-12 parts of poria. According to the invention, by changing the raw materials and the component amount based on the prior art, the efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation disclosed by the invention realizes an obvious treatment effect on pain, blood stasis, cerebral ischemia and the like, and also can adjust the TH1 and TH2 balance, obviously enhance the immunity and reduce the macrophage activity.
Description
Technical field
The present invention relates to tcm field, especially relate to a kind of effective powerful gastrodia tuber eucommia soft capsule or preparation.
Background technology
Rhizoma Gastrodiae is the dry tuber of orchid Rhizoma Gastrodiae Gastrodia elata Bl..Have another name called rhizoma gastrodiae, from mother, water Rhizoma Solani tuber osi etc.After the Beginning of Winter to excavating before Clear and Bright next year (with the winter fiber crops for well), be famous Chinese crude drug.Before two thousand years, be just used as medicine, take Zhaotong County, Yunnan product person as excellent.In Rhizoma Gastrodiae, be rich in gastrodine, Vanillin, protein, aminoacid, trace element.Its property is pungent, warm, nontoxic, has epilepsy, and resisting throbs with fear faints, rheumatism; Calmness, spasmolytic, analgesia, tonify deficiency, the effect of suppressing the hyperactive liver to relieve the wind syndrome.Function cures mainly suppressing the hyperactive liver to relieve the wind syndrome relieving convulsion.Be used for having a headache dizzy, numb limbs and tense tendons, epilepsy clonus, tetanus.Feel dizzy, neurasthenia, anemofrigid-damp arthralgia, the diseases such as infantile convulsion.Clinical practice proves that vascular nervous headache, cerebral concussion sequela etc. are had to significant curative effect.
The Cortex Eucommiae is the dry bark of the Eucommiaceae plant Cortex Eucommiae (Eucommia ulmoides Oliver), is Chinese famous and precious tonic herb.Tool invigorating the liver and kidney, bone and muscle strengthening, blood pressure lowering, many effects such as antiabortive.< < Sheng Nong's herbal classic > > classifies as top grade.Call its " cure mainly waist and knee, invigorating middle warmer, beneficial vital essence, hard muscles and bones, wet except itching under the moon, dribbling urination.Clothes for a long time, make light of one's life by commiting suicide resistance to old." Cortex Eucommiae is Chinese peculiar medical material, its medicinal history is long, clinical, has a wide range of applications.Find on earth so far nearly 14 kinds of Cortex Eucommiae platymisciums, they became extinct in succession in continent and Europe afterwards.Being present in the Chinese Cortex Eucommiae is that the Eucommiaceae Cortex Eucommiae belongs to the Relict Plant of only depositing, and it not only has very high economic worth, and all has very important scientific value for all many-sides such as the origins of research angiosperm phylogeny and Chinese Flora.
Therefore use the Chinese medicine composition of Rhizoma Gastrodiae and the Cortex Eucommiae of a great variety.For example: Chinese Patent Application No. is 02109316.4, name is called " a kind of Chinese medicine and processing technology for the treatment of ache tendon and vessel and numb limbs and tense tendons "; This patent discloses the compositions of 12 taste Chinese medicines such as comprising Rhizoma Gastrodiae, the Cortex Eucommiae.
Chinese Patent Application No. is 200510013685.4, and name is called " a kind of Cortex Eucommiae pharmaceutical composition that contains is treated the application in chronic insufficient cerebral blood supply medicine in preparation ", and this patent also discloses the compositions of 12 taste Chinese medicines such as comprising Rhizoma Gastrodiae, the Cortex Eucommiae.
Above-mentioned composition is all having obvious therapeutical effect to aspects such as pain, blood stasis, cerebral ischemias.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of effective powerful gastrodia tuber eucommia soft capsule or preparation; This soft capsule or preparation are on the basis of existing technology, by the variation of raw material and the variation of group component, not only all there is to obvious therapeutical effect the aspects such as pain, blood stasis, cerebral ischemia, but also the effect of scalable TH1 and TH2 balance, the humoral immunoresponse(HI) that strengthens, reduction macrophage activity.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
Effective powerful gastrodia tuber eucommia soft capsule or preparation, be comprised of the raw material of following parts by weight:
Above-mentioned raw materials is made to capsule or preparation according to existing conventional method, obtain product.
The present invention has following beneficial effect: the present invention is by the variation of raw material and the variation of group component in prior art, not only all there is to obvious therapeutical effect the aspects such as pain, blood stasis, cerebral ischemia, but also the effect of scalable TH1 and TH2 balance, the significant enhancing immunity of energy, reduction macrophage activity.
The specific embodiment
Embodiment 1
Effective powerful gastrodia tuber eucommia soft capsule or preparation, be comprised of the raw material of following parts by weight:
Rhizoma Gastrodiae is worn into fine powder, then mix with the decoction product of other 11 taste medicine, make capsule or the preparation of 0.2g/ grain.
Embodiment 2
Effective powerful gastrodia tuber eucommia soft capsule or preparation, be comprised of the raw material of following parts by weight:
Rhizoma Gastrodiae is worn into fine powder, then mix with the decoction product of other 11 taste medicine, make capsule or the preparation of 0.2g/ grain.
Embodiment 3
Effective powerful gastrodia tuber eucommia soft capsule or preparation, be comprised of the raw material of following parts by weight:
Rhizoma Gastrodiae is worn into fine powder, then mix with the decoction product of other 11 taste medicine, make capsule or the preparation of 0.2g/ grain.
Comparative example 1:
Use the raw material of following umber:
Rhizoma Gastrodiae is worn into fine powder, then mix with the decoction product of other 8 taste medicine, make capsule or the preparation of 0.2g/ grain.
Comparative example 2: be the capsule product of the 0.2g/ grain that obtains of 02109316.4 embodiment 1 according to Chinese Patent Application No..
Comparative example 3: be optimal proportion raw material in 200510013685.4 embodiment according to Chinese Patent Application No., make the capsule product of 0.2g/ grain.
The experiment of product effect:
The product that experiment product 1 obtains for embodiment 1, the product that experiment product 2 obtains for embodiment 2, the product that experiment product 3 obtains for embodiment 3; The capsule that experiment product 4 obtains for comparative example 1; The capsule that experiment product 5 obtains for comparative example 2; The capsule that experiment product 6 obtains for comparative example 3;
I, above-mentioned 6 groups of experiment products are carried out to T lymphocyte transformation test (T LymphocyteTransformation Test) respectively
1, ultimate principle
When T cell is cultivated in vitro, be subject to after mitogen (as PHA, ConA) stimulation, can occur that cell volume increases, metabolism is vigorous, and albumen and nucleic acid are synthetic to be increased, to lymphoblastic transformation and propagation.The height of lymhocyte transformation rate can reflect the cellular immune level of body, therefore can be used as one of index of measuring machine body immunity function.Reading of lymphocyte transformation test result can have form counting method, three kinds of mtt assay and isotope methods; The present invention adopts mtt assay.
Mtt assay is tetramethyl azo azoles salt trace enzyme reaction colorimetry.MTT is a kind of thiazole salt, chemical name 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl bromination tetrazolium, aqueous solution is yellowish orange.Mouse boosting cell is subject to
experiment productafter effect, there is propagation activation, the corresponding rising of mitochondrion succinic dehydrogenase activity in its born of the same parents, MTT participates in reaction as its substrate, form blue formazan (Formazan) particle deposition in cell or cell peripheral, through hydrochloric acid-isopropyl alcohol is blue solution after dissolving, available enzyme mark analyzer is measured the OD value of cell culture, measures wavelength 570nm.According to the size of OD value, calculate cell proliferation degree in reaction system.
2, experiment material
1) ICR mice (Department Of Medicine, Peking University's Experimental Animal Center provides),
2) RPMI1640 culture fluid, Hank ' s liquid,
3) 6 groups of experiment products of the present invention,
4) MTT (1mg/ml is dissolved in the PBS of Ph7.2),
5) 2.5% iodine tincture, 75% ethanol,
6) aseptic sharp suction pipe and graduated pipette,
7) aseptic dissecting instrument,
8) the 96 flat culture plates in hole,
9) 5%CO
2incubator (U.S. NAPCO company product),
10) enzyme mark analyzer (U.S. Bi oRad company product);
3, experimental technique
1) preparation of mouse boosting cell suspension:
Get the plate of a sterilizing, add 5ml Hank ' s liquid.Cervical vertebra dislocation method is put to death mice, gets spleen, puts into plate, grinds and sieve on steel mesh, makes cell suspension.Take out 100ml for counting.All the other cell suspension are moved in a centrifuge tube, centrifugal 1500rpm, 7min, (or 1000rpm, 10min) supernatant discarded, with the dilution of RPMI RPMI-1640, makes the splenocyte suspension of 2.5 × 106/ml, then adding experiment product to make every Kongzui final concentration is 2mg/ml, does the negative control hole that does not add experiment product simultaneously.
2) above-mentioned cell suspension is added in the 96 flat culture plates in hole to every hole 0.1ml.
3) culture plate is put into and contained 5%CO
237 ℃ of incubators in cultivate 48-72 hour, 4-6 hour before cultivation finishes adds 1mg/ml MTT liquid, 10ml/ hole in the each hole of culture plate.Cultivate 6 hours for 37 ℃.
4) in each hole, add 0.01M hydrochloric acid-isopropyl alcohol 110ml, in 30 minutes (or add 2%SDS 100ul/ hole, and spend the night) with enzyme mark analyzer, survey OD value, measure wavelength 570nm.
4, experimental result
By average the OD value in experimental group and three multiple holes of matched group.
The average OD value of the average OD value-matched group of conversion values=experimental group.
Experimental result sees the following form
Experiment conclusion: the present invention finds unexpectedly, be 02109316.4,200510013685.4 and lack Semen Citri Reticulatae with Chinese Patent Application No., the formula of Haematitum and Poria is compared, in the case of the parts by weight ratio that increases substantially Rhizoma Gastrodiae and the Cortex Eucommiae, the present invention can unexpected increase mouse lymphocyte conversion ratio.The present invention has the effect of unexpected enhancing immunity.The present invention finds unexpectedly, by adding Semen Citri Reticulatae, Haematitum and Poria component, makes formula of the present invention obtain unexpected effect.
II, this experimental example prove the pharmacodynamic action of the present composition with the model experiment model of enhancing human body immunity power function.
1, thymus/weight ratio pH-value determination pH test
1) material
1.1) kunming mice, sex is female, 18～22 grams of body weight, source: commercially available.
1.2) experimental drug: the product that experiment product 1 obtains for embodiment 1, the product that experiment product 2 obtains for embodiment 2, the product that experiment product 3 obtains for embodiment 3; The capsule that experiment product 4 obtains for comparative example 1; The capsule that experiment product 5 obtains for comparative example 2; The capsule that experiment product 6 obtains for comparative example 3;
Preparation: experiment product 1-6 is made to suspension with 0.3%CMC-Na respectively.
Modeling is commercially available with cyclophosphamide CTX. source.
1.3) experimental technique
1.3.1) thymus/weight ratio pH-value determination pH
By the large young pathbreaker mice of body weight, be divided into negative control group, model group (intraperitoneal injection of cyclophosphamide causes immunologic hypofunction) and experiment product group 1-6.Each experimental group is pressed relative medicine oral administration, 0.2ml/10g body weight, once a day, successive administration 15 days.11st～14 days intraperitoneal injection of cyclophosphamide 80mg/kg body weight.After last administration in the 15th day about 1 hour, take body weight, with dislocation of cervical vertebra method, put to death mice, get thymus and weigh, calculate thymus/body weight ratio.The each group difference of statistical analysis.The results are shown in following table:
Thymus/weight ratio pH-value determination pH
Experimental result shows, the product of the invention process 1-3 is obviously better than existing product in effect, is also better than not adding Semen Citri Reticulatae, the formula of Haematitum and Poria.Above-mentioned experiment embodies formula combination of the present invention and has unexpected effect.
In addition, by Turnover of Mouse Peritoneal Macrophages, engulf the phagocytic activity of chicken red blood cell measuring macrophage, result shows that product of the present invention can obviously reduce Turnover of Mouse Peritoneal Macrophages and engulf chicken red blood cell percentage rate, shows that product of the present invention can reduce mouse macrophage activity.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here cannot give exhaustive to all embodiments.Everyly belong to apparent variation or the still row in protection scope of the present invention of variation that technical scheme of the present invention extends out.
Claims (1)
1. effective powerful gastrodia tuber eucommia preparation, is characterized in that, the crude drug of following parts by weight, consists of:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210382677.7A CN102920920B (en) | 2012-10-10 | 2012-10-10 | Efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation |
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| CN201210382677.7A CN102920920B (en) | 2012-10-10 | 2012-10-10 | Efficient powerful rhizoma gastrodiae and cortex eucommiae soft capsule or preparation |
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| CN102920920B true CN102920920B (en) | 2014-04-23 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872288A (en) * | 2005-06-03 | 2006-12-06 | 天津天士力制药股份有限公司 | Application of medication composition of containing eucommia in preparing medicine for treating insufficiency of blood supply for brain |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872288A (en) * | 2005-06-03 | 2006-12-06 | 天津天士力制药股份有限公司 | Application of medication composition of containing eucommia in preparing medicine for treating insufficiency of blood supply for brain |
Non-Patent Citations (2)
| Title |
|---|
| 余建国 等.茯苓多糖对雏鸡细胞免疫活性的影响及其抗肿瘤作用.《中国兽医科技》.2004,第34卷(第11期),第70-73页. |
| 茯苓多糖对雏鸡细胞免疫活性的影响及其抗肿瘤作用;余建国 等;《中国兽医科技》;20041231;第34卷(第11期);第70-73页 * |
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