CN102920919A - Traditional Chinese medicine composition, preparation method and uses thereof - Google Patents
Traditional Chinese medicine composition, preparation method and uses thereof Download PDFInfo
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Abstract
The present invention provides a traditional Chinese medicine composition, which is prepared from salvia miltiorrhiza, cape jasmine, red peony root, tree peony root-bark and other bulk drugs. The traditional Chinese medicine composition is provided for treatments of obstruction of collaterals by blood stasis and kidney asthenia at a meridian collateral convalescent period of stroke, and syndromes of hemiparalysis, deviation of mouth and tongue, stiff tongue, hemianesthesia, dizziness, soreness and weakness of waist and knees, and the like at a convalescent period of brain infarction. In addition, the present invention further discloses a preparation method for the traditional Chinese medicine composition.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, particularly relate to a kind of hold concurrently Chinese medicine composition that is prepared from by Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae etc. of syndrome of deficiency of kidney yin of stroke in convalescent stage congestion resistance network that is used for the treatment of, the present invention also relates to preparation method and the purposes of this Chinese medicine composition.
Background technology
Suffering from acute stroke, convalescent period, sequela stage all have different characteristics from theory of Chinese medical science or from modern medicine pathology aspect.Apoplexy is that deficiency in origin worsens and the real result who intensifies of mark, its deficiency in origin problem can not be after apoplexy short acute stage be resolved, this also is the main cause that produces sequela.Therefore must be for the deficiency in origin problem in convalescent period, balance specimen weight is paid attention to treating both the principal and the secondary aspects of a disease at the same time.And at present many for the Chinese patent medicine of apoplexy, but there are the following problems: 1. the applicable medicine of stroke in convalescent stage is few; 2. treat the cerebral thrombosis medicine more, and clearly take another common cause of apoplexy sequela---hemiplegia due to the cerebral hemorrhage as the medicine of indication seldom; 3. from TCM Syndrome Type, the medicine that adapts to blood stasis due to qi deficiency disease is more, and it is less to be adapted to the medicine of convalescent period and other common pattern of syndrome of sequela stage.Therefore, for the etiology and pathogenesis characteristics of each phase of apoplexy, develop the serial complete Chinese patent medicine meet clinical needs in the urgent need to.
Summary of the invention
One object of the present invention is to provide a kind of hold concurrently Chinese medicine composition of syndrome of deficiency of kidney yin of stroke in convalescent stage congestion resistance network for the treatment of; Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine composition.
Purpose of the present invention is achieved through the following technical solutions:
Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Gastrodiae, Cortex Moutan, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Rehmanniae, Herba Epimedii, Herba Taxilli, Fructus Gardeniae, Semen Cassiae, Fructus Cannabis by certain compatibility requirement, are processed into through special process.
The inventor provides a kind of Chinese medicine composition, is prepared from by the raw material of following weight portion:
Radix Salviae Miltiorrhizae 300-700 part Radix Achyranthis Bidentatae 240-560 part Rhizoma Gastrodiae 60-140 part Cortex Moutan 200-468 part
Radix Paeoniae Rubra 240-560 part Rhizoma Chuanxiong 100-234 part Radix Rehmanniae 240-560 part Herba Epimedii 180-420 part
Herba Taxilli 240-560 part Fructus Gardeniae 120-280 part Semen Cassiae 120-280 part Fructus Cannabis 120-280 part
The proportioning raw materials of this Chinese medicine composition is preferably:
Radix Salviae Miltiorrhizae 450-550 part Radix Achyranthis Bidentatae 360-440 part Rhizoma Gastrodiae 90-110 part Cortex Moutan 300-370 part
Radix Paeoniae Rubra 360-440 part Rhizoma Chuanxiong 150-185 part Radix Rehmanniae 360-440 part Herba Epimedii 270-330 part
Herba Taxilli 360-440 part Fructus Gardeniae 180-220 part Semen Cassiae 180-220 part Fructus Cannabis 180-220 part
The proportioning raw materials of this Chinese medicine composition is more preferably:
334 parts of 100 parts of Cortex Moutans of 400 parts of Rhizoma Gastrodiaes of 500 parts of Radix Achyranthis Bidentataes of Radix Salviae Miltiorrhizae
300 parts of 400 parts of Herba Epimedii of 167 portions of Radix Rehmanniae of 400 parts of Rhizoma Chuanxiongs of Radix Paeoniae Rubra
200 parts of 200 parts of Fructus Cannabiss of 200 parts of Semen Cassiaes of 400 portions of Fructus Gardeniaes of Herba Taxilli
The preparation method of Chinese medicine composition of the present invention may further comprise the steps: Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Gastrodiae, Cortex Moutan, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Rehmanniae, Herba Epimedii, Herba Taxilli, Fructus Gardeniae, Semen Cassiae, Fructus Cannabis 12 flavors, get Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice and be broken into coarse powder, with 50-85% alcohol reflux secondary, each return time 1-2 hour, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, decoct with water secondary, boiled 1-2 hour for the first time, 1-2 hour for the second time, collecting decoction filtered, filtrate is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), merge with above-mentioned clear paste, being concentrated into relative density is 1.20-1.25 (20 ℃), makes required dosage formulation with pharmaceutically acceptable adjuvant thereafter.
The preparation method of Chinese medicine composition of the present invention can be: Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Gastrodiae, Cortex Moutan, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Rehmanniae, Herba Epimedii, Herba Taxilli, Fructus Gardeniae, Semen Cassiae, Fructus Cannabis 12 flavors, get Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice and be broken into coarse powder, with 70% alcohol reflux secondary, each return time 1 hour, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, decoct with water secondary, boiled 1.5 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), merge with above-mentioned clear paste, being concentrated into relative density is 1.20-1.25 (20 ℃), makes required dosage formulation with pharmaceutically acceptable adjuvant thereafter.
Made dosage form comprises tablet, capsule, granule, pill or oral liquid in the preparation method of Chinese medicine composition of the present invention.
Used adjuvant is lactose in the preparation method of Chinese medicine composition of the present invention, dextrin, starch, sucrose, microcrystalline Cellulose, methylcellulose, hyprolose, hypromellose, carmellose, carmethose, ethyl cellulose, polyvidone, gelatin, Polyethylene Glycol, carboxymethyl starch sodium, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose, polyvinylpolypyrrolidone, sodium bicarbonate, citric acid, magnesium stearate, micropowder silica gel, Pulvis Talci, hydrogenated vegetable oil, ethanol, in the Mel one or more.
Chinese medicine composition of the present invention is mainly used in the treatment treatment stroke in convalescent stage congestion resistance network syndrome of deficiency of kidney yin of holding concurrently.CT scan comparative study confirms before and after the clinical treatment, and Chinese medicine composition of the present invention has the cephalophyma of promotion Absorption, and Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, Radix Paeoniae Rubra, Cortex Moutan, Radix Achyranthis Bidentatae are improved microcirculation and the confession of brain blood, the effect of anticoagulant and promotion fibrinolytic.Herba Epimedii, Herba Taxilli can promote the recovery of function of brain cell.Rhizoma Gastrodiae, Cortex Moutan, Radix Achyranthis Bidentatae, Herba Taxilli, Semen Cassiae have hypotensive effect, and can remove cerebral vasospasm, improve the confession of brain blood and promote resuming function of brain cell.
The specific embodiment
Embodiment 1
Prescription:
Radix Salviae Miltiorrhizae 300g Radix Achyranthis Bidentatae 240g Rhizoma Gastrodiae 60g Cortex Moutan 200g
Radix Paeoniae Rubra 240g Rhizoma Chuanxiong 100g Radix Rehmanniae 240g Herba Epimedii 180g
Herba Taxilli 240g Fructus Gardeniae 120g Semen Cassiae 120g Fructus Cannabis 120g
Method for making: above 12 flavors, get Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice and be broken into coarse powder, with 50% alcohol reflux secondary, each return time 1 hour, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, and decoct with water secondary, boil 1 hour for the first time, 1 hour for the second time, collecting decoction filtered, filtrate is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), merges with above-mentioned clear paste, and being concentrated into relative density is 1.20-1.25 (20 ℃), adding starch, lactose, carboxymethyl starch sodium mix, ethanol is binding agent, and soft material processed is granulated, dry, granulate, add an amount of magnesium stearate, mix, be pressed into 2000.
Embodiment 2
Prescription:
Radix Salviae Miltiorrhizae 350g Radix Achyranthis Bidentatae 280g Rhizoma Gastrodiae 70g Cortex Moutan 234g
Radix Paeoniae Rubra 280g Rhizoma Chuanxiong 117g Radix Rehmanniae 280g Herba Epimedii 210g
Herba Taxilli 280g Fructus Gardeniae 140g Semen Cassiae 140g Fructus Cannabis 140g
Method for making: Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Gastrodiae, Cortex Moutan, Radix Paeoniae Rubra, Rhizoma Chuanxiong, Radix Rehmanniae, Herba Epimedii, Herba Taxilli, Fructus Gardeniae, Semen Cassiae, Fructus Cannabis 12 flavors, get Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice and be broken into coarse powder, with 85% alcohol reflux secondary, each return time 2 hours, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, decoct with water secondary, boiled 2 hours for the first time, 2 hours for the second time, collecting decoction filters, and filtrate is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), merge with above-mentioned clear paste, being concentrated into relative density is 1.20-1.25 (20 ℃), spray drying, add microcrystalline Cellulose, lactose, low-substituted hydroxypropyl cellulose mix, take water as binding agent, soft material processed, granulate, dry, granulate add micropowder silica gel, mix, be filled to 2000 capsules.
Embodiment 3
Prescription
Radix Salviae Miltiorrhizae 500g Radix Achyranthis Bidentatae 400g Rhizoma Gastrodiae 100g Cortex Moutan 334g
Radix Paeoniae Rubra 400g Rhizoma Chuanxiong 167g Radix Rehmanniae 400g Herba Epimedii 300g
Herba Taxilli 400g Fructus Gardeniae 200g Semen Cassiae 200g Fructus Cannabis 200g
Method for making: above 12 flavors, Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice are broken into coarse powder, with 70% alcohol reflux secondary, each return time 1 hour, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, and decoct with water secondary, boil 1.5 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), merges with above-mentioned clear paste, and being concentrated into relative density is 1.20-1.25 (20 ℃), add sucrose, dextrin mixes, take water as binding agent, soft material processed, granulate, dry, granulate are made 10000g.
Embodiment 4
Prescription
Radix Salviae Miltiorrhizae 450g Radix Achyranthis Bidentatae 360g Rhizoma Gastrodiae 90g Cortex Moutan 300g
Radix Paeoniae Rubra 360g Rhizoma Chuanxiong 150g Radix Rehmanniae 360g Herba Epimedii 270g
Herba Taxilli 360g Fructus Gardeniae 180g Semen Cassiae 180g Fructus Cannabis 180g
Method for making: above 12 flavors, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong four flavors are ground into coarse powder, with 70% alcohol reflux secondary, refluxed 2 hours for the first time, refluxed 1 hour for the second time,, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, and decoct with water secondary, boil 2 hours for the first time, 1 hour for the second time, collecting decoction filters, and filtrate is concentrated into the thick paste that relative density is about 1.20-1.25 (20 ℃), Radix Achyranthis Bidentatae is ground into fine powder and above-mentioned thick paste mix homogeneously, the pill bar, gradation, round, drying is made 1300 balls.
Embodiment 5
Prescription
Radix Salviae Miltiorrhizae 550g Radix Achyranthis Bidentatae 440g Rhizoma Gastrodiae 110g Cortex Moutan 370g
Radix Paeoniae Rubra 440g Rhizoma Chuanxiong 185g Radix Rehmanniae 440g Herba Epimedii 330g
Herba Taxilli 440g Fructus Gardeniae 220g Semen Cassiae 220g Fructus Cannabis 220g
Method for making: above 12 flavors, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong four flavors are ground into coarse powder, with 70% alcohol reflux secondary, refluxed 1 hour for the first time, refluxed 2 hours for the second time, filter, filtrate recycling ethanol also is concentrated into the clear paste that relative density is about 1.05-1.15 (60 ℃), and is for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, and decoct with water secondary, boil 1 hour for the first time, 2 hours for the second time, collecting decoction filters, and filtrate is concentrated into the thick paste that relative density is about 1.20-1.25 (20 ℃), adding ethanol makes it contain alcohol amount to reach 75%, left standstill 24 hours, and filtered, filtrate is concentrated into 20000ml, be distributed into 500, and get final product.
Embodiment 6
Select experimenter's 77 examples, after random assortment enters test group and matched group, test group 57, matched group 20 examples are accepted test or the control drug treatment in 12 weeks, take the granule of embodiment 3 as the test group medication, each serving 10g, every day 3 times, matched group is taken 6 of XIAOSHUAN TONGLUO PIAN, every day 3 times, all must not use simultaneously the other medicines relevant with primary disease for two groups.4 weeks were a course for the treatment of.
One) efficacy assessment standard
The neurologic impairment efficacy assessment standard: full marks are 28 minutes before the treatment, its efficacy evaluation employing nimodipine method: therapeutic index n=(score after score before the treatment-treatment)/front score for the treatment of * 100%
1. be almost recovered: 〉=85%; 2. produce effects: 〉=50%; 3. effective: 〉=20%; 4. invalid:＜20%
Table neurologic impairment scoring criteria
(2) traditional Chinese medical science disease efficacy assessment standard
Score * 100% before therapeutic index n=(score after score before the treatment-treatment)/treatment
1. be almost recovered: 〉=90%; 2. produce effects: 〉=70%; 3. effective: 〉=30%; 4. invalid:＜30%
Table tcm symptom scoring criteria
(3) efficacy analysis
1. neurologic impairment efficacy analysis
Table two group neurologic impairment is always scored the improvement situation relatively
Self significant difference is arranged before and after the experimental group treatment as seen from the above table, p＜0.01, experimental group and matched group are always scored the improvement situation relatively to neurologic impairment, significant difference are arranged, p＜0.05.
Table two group neurologic impairment total effects relatively
The experimental group total effective rate 91.23% as seen from the above table, and cure-remarkable-effectiveness rate is 50.87%, compares with matched group, significant difference arranged, p＜0.05.
2. tcm syndrome efficacy analysis
Table two group Syndrome in TCM Hou always scores the improvement situation relatively
Self significant difference is arranged before and after the experimental group treatment as seen from the above table, p＜0.01, experimental group and matched group are always scored the improvement situation relatively to tcm syndrome, there was no significant difference, p＞0.05.
Table two group tcm syndrome total effects relatively
The experimental group total effective rate 92.98% as seen from the above table, and cure-remarkable-effectiveness rate is 21.05%, compares with matched group, significant difference arranged, p＜0.05.
Claims (10)
1. Chinese medicine composition is characterized in that being prepared from by the raw material of following weight portion:
Radix Salviae Miltiorrhizae 300-700 part Radix Achyranthis Bidentatae 240-560 part Rhizoma Gastrodiae 60-140 part Cortex Moutan 200-468 part
Radix Paeoniae Rubra 240-560 part Rhizoma Chuanxiong 100-234 part Radix Rehmanniae 240-560 part Herba Epimedii 180-420 part
Herba Taxilli 240-560 part Fructus Gardeniae 120-280 part Semen Cassiae 120-280 part Fructus Cannabis 120-280 part.
2. Chinese medicine composition is characterized in that being prepared from by the raw material of following weight portion:
Radix Salviae Miltiorrhizae 450-550 part Radix Achyranthis Bidentatae 360-440 part Rhizoma Gastrodiae 90-110 part Cortex Moutan 300-370 part
Radix Paeoniae Rubra 360-440 part Rhizoma Chuanxiong 150-185 part Radix Rehmanniae 360-440 part Herba Epimedii 270-330 part
Herba Taxilli 360-440 part Fructus Gardeniae 180-220 part Semen Cassiae 180-220 part Fructus Cannabis 180-220 part.
3. Chinese medicine composition according to claim 1 is characterized in that being prepared from by the raw material of following weight portion:
334 parts of 100 parts of Cortex Moutans of 400 parts of Rhizoma Gastrodiaes of 500 parts of Radix Achyranthis Bidentataes of Radix Salviae Miltiorrhizae
300 parts of 400 parts of Herba Epimedii of 167 portions of Radix Rehmanniae of 400 parts of Rhizoma Chuanxiongs of Radix Paeoniae Rubra
200 parts of 200 parts of Fructus Cannabiss of 200 parts of Semen Cassiaes of 400 portions of Fructus Gardeniaes of Herba Taxilli.
4. the preparation method of the described Chinese medicine composition of arbitrary claim in the claims 1 to 3, it is characterized in that passing through following steps: above 12 flavors, Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice are broken into coarse powder, with 50-85% alcohol reflux secondary, each return time 1-2 hour, filter, filtrate recycling ethanol also is concentrated into relative density and is about 1.05-1.15(60 ℃) clear paste, for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, decoct with water secondary, boiled 1-2 hour for the first time, 1-2 hour for the second time, collecting decoction filtered, filtrate is concentrated into relative density and is about 1.05-1.15(60 ℃) clear paste, merge with above-mentioned clear paste, being concentrated into relative density is 1.20-1.25(20 ℃), make required dosage formulation with pharmaceutically acceptable adjuvant thereafter.
5. the preparation method of described Chinese medicine composition according to claim 4, it is characterized in that passing through following steps: above 12 flavors, Radix Achyranthis Bidentatae, Cortex Moutan, Radix Salviae Miltiorrhizae, Fructus Cannabis, Rhizoma Chuanxiong ground spice are broken into coarse powder, with 70% alcohol reflux secondary, each return time 1 hour, filter, filtrate recycling ethanol also is concentrated into relative density and is about 1.05-1.15(60 ℃) clear paste, for subsequent use; All the other seven flavors such as medicinal residues and Radix Rehmanniae merge, decoct with water secondary, boiled 1.5 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate is concentrated into relative density and is about 1.05-1.15(60 ℃) clear paste, merge with above-mentioned clear paste, being concentrated into relative density is 1.20-1.25(20 ℃), make required dosage formulation with pharmaceutically acceptable adjuvant thereafter.
6. the preparation method of Chinese medicine composition according to claim 4 is characterized in that making tablet, capsule, granule, pill or oral liquid.
7. the preparation method of Chinese medicine composition according to claim 5 is characterized in that making tablet, capsule, granule, pill or oral liquid.
8. the preparation method of described Chinese medicine composition according to claim 4 is characterized in that used adjuvant is one or more in lactose, dextrin, starch, sucrose, microcrystalline Cellulose, methylcellulose, hyprolose, hypromellose, carmellose, carmethose, ethyl cellulose, polyvidone, gelatin, Polyethylene Glycol, carboxymethyl starch sodium, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose, polyvinylpolypyrrolidone, sodium bicarbonate, citric acid, magnesium stearate, micropowder silica gel, Pulvis Talci, hydrogenated vegetable oil, ethanol, the Mel.
9. the preparation method of Chinese medicine composition according to claim 5 is characterized in that used adjuvant is one or more in lactose, dextrin, starch, sucrose, microcrystalline Cellulose, methylcellulose, hyprolose, hypromellose, carmellose, carmethose, ethyl cellulose, polyvidone, gelatin, Polyethylene Glycol, carboxymethyl starch sodium, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose, polyvinylpolypyrrolidone, sodium bicarbonate, citric acid, magnesium stearate, micropowder silica gel, Pulvis Talci, hydrogenated vegetable oil, ethanol, the Mel.
10. claim 1, the application of 2 or 3 described Chinese medicine compositions in preparation stroke in convalescent stage medicine.
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Cited By (1)
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| CN103989960A (en) * | 2013-02-18 | 2014-08-20 | 九芝堂股份有限公司 | Traditional Chinese medicine composition and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1136935A (en) * | 1995-05-29 | 1996-12-04 | 彭敏球 | Medicinal granule for treating hemiparalysis and producing process thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1136935A (en) * | 1995-05-29 | 1996-12-04 | 彭敏球 | Medicinal granule for treating hemiparalysis and producing process thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103989960A (en) * | 2013-02-18 | 2014-08-20 | 九芝堂股份有限公司 | Traditional Chinese medicine composition and preparation method thereof |
| CN103989960B (en) * | 2013-02-18 | 2018-06-08 | 九芝堂股份有限公司 | A kind of Chinese medicine composition and preparation method thereof |
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Application publication date: 20130213 |