CN102919196B - Breeding method capable of improving milk yield of dairy cows and quality of produced milk - Google Patents
Breeding method capable of improving milk yield of dairy cows and quality of produced milk Download PDFInfo
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- CN102919196B CN102919196B CN201210445743.0A CN201210445743A CN102919196B CN 102919196 B CN102919196 B CN 102919196B CN 201210445743 A CN201210445743 A CN 201210445743A CN 102919196 B CN102919196 B CN 102919196B
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Abstract
The invention discloses a breeding method capable of improving milk yield of dairy cows and quality of produced milk and application of acid production propionibacterium in preparing supplement feeds for improving milk yield of dairy cows and quality of produced milk. The breeding method is that before lactation period cows are fed general feeds, each cow is fed 5-100g of acid production propionibacterium every day. Preferably, each cow is fed 20-40g of acid production propionibacterium every day. By means of the breeding method, milk yield of lactation period cows is improved, and lactose and milk fat content in the produced mil is further improved. In addition, the breeding method has good industrial value.
Description
Technical field
The present invention relates to a kind of improve milk production of cow, milk quality raising method and produce sour Propionibacterium preparation improve milk production of cow, milk quality supplement feed in application.
Background technology
In recent years, along with China's economic continue, stable development and growth in the living standard per capita, country and each place have listed the industry of prominent development in milk industry one after another.In recent years, the scale of domestic milk product enterprise increased substantially, from 2000 to 2005, and milk increase of production 211.7%, but compared with developed countries, the overall development level of China milk industry is also not high.Analyze according to authoritative institution, China town dweller pre-capita consumption also only has about 20 kilograms every year, is 1/5 of world average level, and developed country per capita annual consumption figure then reaches 320 kilograms, is 16 times of China.Therefore, the development of China milk industry also exists great potentiality and the market space.
From milk cow unit yield, current China milk cow annual per unit area yield only has about 4000 kilograms, is about the half of U.S.'s milk ox year per unit area yield.From milk quality, in milk composition, major part is water, and account for 86% ~ 89%, dry matter accounts for 11% ~ 14%; Dry matter content is directly relevant with the content of composition its butter oil, milk protein, lactose etc.Because butterfat percnetage and protein ratio, dry matter and heat content etc. are all proportionate, and butterfat is the Main Factors of left and right flavor of dairy products, so usually butterfat percnetage is considered as the mark of newborn quality.Under normal circumstances, milk yield improves the normal decline with component contents such as butter oils.In prior art, have and use yeast culture to improve the report of milk production of cow and lactose and dairy fat content.Yeast culture by improving the immunity of milk cow, improving the ability of the microbial digestion feed of cud and enteron aisle, thus can improve lactose, the dairy fat content in the milk yield of milk cow and milk.But the scale of feeding of yeast culture is large, and cost is high, and also limited for the raising effect of milk production of cow and lactose, dairy fat content.
Therefore, find a kind ofly can improve milk production of cow, the raising method of milk quality become cow estate urgent problem.
Summary of the invention
The technical problem to be solved in the present invention is for providing a kind of raising method improving milk production of cow, milk quality.
Particularly, the raising method of above-mentioned raising milk production of cow, milk quality is, for milk cow in lactation period, before giving conventional feed, gives the product acid Propionibacterium mycoprotein of totally 5 ~ 100g every cow head every day.
Preferably, for milk cow in lactation period, before giving conventional feed, give the product acid Propionibacterium mycoprotein of totally 20 ~ 40g every cow head every day.
More preferably, for milk cow in lactation period, before giving conventional feed, give the product acid Propionibacterium mycoprotein that every cow head is total to 30g every day.
In above-mentioned raising method, described product sour Propionibacterium mycoprotein is taken every day at twice, sooner or later respectively feeds once.
The preparation technology of the sour Propionibacterium mycoprotein of described product:
To produce sour Propionibacterium (Propionibacterium acidipropionici) CGMCC1.2232 for bacterial classification, carry out as follows cultivating, extracting:
(1) seed culture:
By the glycerine pipe bacterial classification of preservation according to 0.1 ~ 2% inoculum concentration be inoculated in (the bottled 200mL seed culture medium of 250mL anaerobism) in anaerobism bottle, in anaerobism bottle after inflated with nitrogen, be placed in 20-40 DEG C of incubator quiescent culture 36-60h; Seed culture medium consists of: dusty yeast 5-25g/L, Tryptic soy broth5-15g/L, K
2hPO
40.5-3.5g/L, KH
2pO
40.1-2.5g/L.
(2) fermentation tank culture:
The above-mentioned seed being cultured to logarithmic phase is inoculated in fermentation in 300L fermentation tank (liquid amount 250L) according to 5-20% inoculum concentration, fermentation temperature 20-40 DEG C, add 5-40%(w/v by stream) NaOH, pH is maintained 6.5-7.5, speed of agitator 50-150rpm, passes into nitrogen continuously and maintains anaerobic environment.To bacterium amount no longer increases, stop fermentation, incubation time 24-48 hour; Fermentation medium consists of: Dried Corn Steep Liquor Powder 10-25g/L, soybean meal 5-15g/L, K
2hPO
40.5-3.5g/L, KH
2pO
40.1-2.5g/L, glycerine 10-50g/L; Described Dried Corn Steep Liquor Powder, soybean meal all obtain by commercialization approach.
(3) mycoprotein extraction process:
By fermentation using propionibacterium liquid, by plate compression, obtain mycoprotein, 40-50 DEG C, dry in low temperature drying case, obtain producing sour Propionibacterium mycoprotein powder.
In above-mentioned raising method, described conventional feed is corn silage mixed ration.
By weight, consisting of of described corn silage mixed ration: corn silage 25.37%, wheat 37.23%, dregs of beans cotton seed 7.53%, corn bran feed 7.88%, alfalfa 20.85%, salt 0.18%, calcium carbonate 0.39%, sodium bicarbonate 0.39%, magnesia 0.18%.
Preferably, described corn silage mixed ration administered dose is 3.5 ~ 5.5kg/ head/sky.
Present invention also offers a kind of produce sour Propionibacterium preparation improve milk production of cow, milk quality supplement feed in application.
According to above-mentioned application, in a specific embodiment, for milk cow in lactation period, before giving conventional feed, give the product acid Propionibacterium mycoprotein of totally 5 ~ 100g every cow head every day.
Preferably, for milk cow in lactation period, before giving conventional feed, give the product acid Propionibacterium mycoprotein of totally 20 ~ 40g every cow head every day.
More preferably, for milk cow in lactation period, before giving conventional feed, give the product acid Propionibacterium mycoprotein that every cow head is total to 30g every day.
Raising method of the present invention can significantly improve the average daily milk yield of milk cow, the lactose of milk, dairy fat content, compared with blank group, the highlyest can improve 49.1%, 115%, 86.5% respectively, also apparently higher than milk yield and milk lactose, the dairy fat content of yeast culture raising group; In addition, the sour Propionibacterium mycoprotein of raising method of the present invention product used can be propionic fermentation accessory substance, and its cost is low, and scale of feeding is little.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1 produces the impact of sour Propionibacterium on milk cow in lactation period milk yield, milk quality
Select the holstein cow of first calving, feeding cycle is for giving a birth first 2 weeks to 30 weeks postpartum.Experiment is divided into six groups and carries out, and often organizes 10 cow heads.By weight, consisting of of the following stated corn silage mixed ration: corn silage 25.37%, wheat 37.23%, dregs of beans cotton seed 7.53%, corn bran feed 7.88%, alfalfa 20.85%, salt 0.18%, calcium carbonate 0.39%, sodium bicarbonate 0.39%, magnesia 018%.
Blank group is: corn silage mixed ration (4kg/ head/sky), and every day respectively feeds once sooner or later;
Yeast culture group is: yeast culture (50g/ head/sky) and corn silage mixed ration (4kg/ head/sky), and every day respectively feeds once sooner or later, when feeding, first gives yeast culture at every turn, then gives corn silage mixed ration;
Produce sour bacterium acidi propionici mycoprotein group, 5g/ head/sky group is divided into according to feeding volume difference, 20g/ head/sky group, 30g/ head/sky group, 100g/ head/sky group: produce sour Propionibacterium mycoprotein (5g/ head/sky, 20g/ head/sky, 30g/ head/sky, 100g/ head/sky) and corn silage mixed ration (4kg/ head/sky), every day respectively feeds once, when feeding sooner or later at every turn, first produce sour Propionibacterium mycoprotein, then give corn silage mixed ration.
Record the daily milk yield of each group milk cow in feeding cycle, and the lactose measured in milk and dairy fat content, respectively organize average lactose in the average daily milk yield of milk cow, milk, dairy fat content is as shown in table 1.
The assay of lactose, butterfat is respectively according to the detecting about the assay method of fat " in infant food and the dairy products " about the assay method of lactose, sucrose " in infant food and the dairy products " and GB5413.3-2010 regulation of national food safety standard GB5413.5-2010 regulation.
Average lactose content, average dairy fat content in table 1, the average daily milk yield respectively organizing milk cow, milk
As shown in Table 1, the feed milk yield of group, lactose content and dairy fat content of the product acid Propionibacterium mycoprotein of Different adding amount is all significantly improved than feed group and blank group of yeast culture, integrated cost factor, the feed effect of group of wherein 30g/ head/sky Propionibacterium mycoprotein is best, and contrast yeast culture is fed group and blank elements indescribably high 30.5% and 49.1%; 75.5% and 115%; 64.3% and 86.5%.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (3)
1. improve a raising method for milk production of cow, milk quality, it is characterized in that, for milk cow in lactation period, before giving conventional feed, only give the product acid Propionibacterium mycoprotein of totally 5 ~ 100g every cow head every day;
Described conventional feed is corn silage mixed ration; Consisting of of described corn silage mixed ration: corn silage 25.37%, wheat 37.23%, dregs of beans cotton seed 7.53%, corn bran feed 7.88%, alfalfa 20.85%, salt 0.18%, calcium carbonate 0.39%, sodium bicarbonate 0.39%, magnesia 0.18%; The administered dose of described corn silage mixed ration is 3.5-5.5kg/ head/sky.
2. raising method as claimed in claim 1, is characterized in that, for milk cow in lactation period, before giving conventional feed, gives the product acid Propionibacterium mycoprotein of totally 20 ~ 40g every cow head every day.
3. raising method as claimed in claim 1, is characterized in that, for milk cow in lactation period, before giving conventional feed, gives the product acid Propionibacterium mycoprotein that every cow head is total to 30g every day.
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| CN201210445743.0A CN102919196B (en) | 2012-11-08 | 2012-11-08 | Breeding method capable of improving milk yield of dairy cows and quality of produced milk |
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| CN102919196B true CN102919196B (en) | 2015-02-18 |
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| CN105901352A (en) * | 2016-05-06 | 2016-08-31 | 山东新希望六和集团有限公司 | Feed additive for improving milk yield of milk cow and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703146A (en) * | 2002-10-01 | 2005-11-30 | 营养生理公司 | Compositions and methods for inhibiting pathogenic growth |
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| US6951643B2 (en) * | 2001-07-24 | 2005-10-04 | Oklahoma State University | Direct-fed microbial |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703146A (en) * | 2002-10-01 | 2005-11-30 | 营养生理公司 | Compositions and methods for inhibiting pathogenic growth |
Non-Patent Citations (4)
| Title |
|---|
| Effects of Diet on Short-Term Regulation of Feed Intake by Lactating Dairy Cattle;Michael S. Allen;《J Dairy Sci》;20000731;第83卷(第7期);第1598-1624页 * |
| Effects of feeding yeast and propionibacteria to dairy cows on milk yield and components, and reproduction;K. V. Lehloenya et al.;《Journal of Animal Physiology and Animal Nutrition》;20080430;第92卷(第2期);第190-202页 * |
| 奶牛微生态制剂几种益生菌生物学特性的研究;王美秀等;《畜牧与饲料科学》;20060531(第5期);第1-5页 * |
| 小麦在反刍动物饲料中的应用研究进展;卢萍等;《粮食与饲料工业》;20030930(第9期);第30-32页 * |
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