CN102919171A - Method for distinguishing parent freshwater shrimps from autumn breeding seedlings - Google Patents
Method for distinguishing parent freshwater shrimps from autumn breeding seedlings Download PDFInfo
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- CN102919171A CN102919171A CN2012104327511A CN201210432751A CN102919171A CN 102919171 A CN102919171 A CN 102919171A CN 2012104327511 A CN2012104327511 A CN 2012104327511A CN 201210432751 A CN201210432751 A CN 201210432751A CN 102919171 A CN102919171 A CN 102919171A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a method for distinguishing parent freshwater shrimps and autumn breeding seedlings, specifically relates to a method for distinguishing parent freshwater shrimps and autumn breeding seedlings by using a neutral red vital staining method and belongs to the technical field of fishing breeding. According to the method, neutral red is used as a material, an optimum dye concentration and optimum dyeing time for vital staining of the freshwater shrimps by using the neutral red are successfully figured out, and a vital staining method of the freshwater shrimps, which has good effects, is obtained. The method is used for distinguishing the parent freshwater shrimps and the autumn breeding seedlings to reduce probability of inbreeding so as to protect germplasm resources of the freshwater shrimps and achieve continuous and health development of the freshwater shrimp breeding industry, a theoretical basis is established for elite breeding of the freshwater shrimps, and an application of other vital dyes to vital staining of the freshwater shrimps is promoted.
Description
Technical field
The present invention relates to numerous differentiation of seedling method of a kind of parent freshwater shrimp and autumn, be specifically related to a kind of dimethyl diaminophenazine chloride vital staining method of passing through to distinguish the method for parent freshwater shrimp and Qiu Fanmiao, belong to fishery propagation technique field.
Background technology
Freshwater shrimp, formal name used at school Japanese macrobrachium supernbum (Macrobrachium nipponense) is subordinate to Decapoda (Decapoda), and Palaemonidae (Palaemonidae), pond crayfish belong to (Macrobrachium), being distributed widely in all parts of the country, is the important freshwater aquiculture shrimps of China.In recent years, along with the continuous expansion of aquaculture industry, freshwater shrimp germ plasm resource is degenerated serious, and the phenomenons such as specification is little, sex premature become the key issue of restriction shrimp culture industry sustainable development.
The freshwater shrimp early sexual maturity, fertility is strong, but annual 4-9 month equal egg reproductions.Autumn oogenesis, the nutrition that close shrimp is daily to ingest is most of to be used for embryonic development, growth rate slows down naturally; Moreover numerous seedling of a large amount of autumns and the close shrimp of generation strive food, strive the space, will inevitably mutually restrict growth, have a strong impact on the economic benefit of shrimp culture.And Qiu Fanmiao has a lot of adverse influences to the protection of freshwater shrimp germ plasm resource, has hindered to a great extent continuing, developing in a healthy way of shrimp culture.It is that single household is from numerous self-fertile mostly that the freshwater shrimp seed produces, seed shrimp is without seed selection, some raiser takes disposablely to put seedling, fish for the whole year or fish for for many years, cause the same pool of freshwater shrimp three generations, inbred probability increases, and the degradation phenomena of freshwater shrimp germplasm is progressively obvious, be embodied in individual less than normal, fish brood amount reduces, and sexual maturity is done sth. in advance, degradation under the premunition.On the other hand, the unordered of freshwater shrimp seed flowed, and germplasm mixes, and escapes seedling and enters the river to the pollution of Natural resources reservoir etc., and the hydridization of shrimp seedling is self-evident, the degeneration of germplasm and hydridization, the vicious circle that will cause seed to produce, the difficult quality guarantee of seed.The key that solves the degeneration of freshwater shrimp germ plasm resource is to find out a reasonable approach to distinguish freshwater shrimp parent and Qiu Fanmiao, reduces inbred probability, to reach the protection of freshwater shrimp germ plasm resource, realizes continuing of shrimp culture industry, sound development.
Vital staining (vital staining) generally is to select dyestuff nontoxic or that toxicity is very little, under the prerequisite of the normal activities that does not affect cell and make the painted a kind of experimental technique of some structure of living cells, that carry out the earliest vital staining is Paul Ehrlich(1885) his flesh tissue that downcuts looking down from a height is immersed in methylene blue (methylene blue) solution, observes the living cells structure that still is under the animation with microscope again.Dimethyl diaminophenazine chloride (Neutral red) is a kind of basic phenazine dye of cell vital staining and Acidity of Aikalinity indicator.Dimethyl diaminophenazine chloride is that a kind of alkalescent pH indicator is in neutrality or alkalescence environment, the living cells of plant can absorb dimethyl diaminophenazine chloride and excretion in the vacuole in a large number, because vacuole reacts acid in the ordinary course of things, therefore, the dimethyl diaminophenazine chloride that enters vacuole just dissociates a large amount of cations and presents cherry-red, in this case, protoplasm and cell wall are generally not painted; Dead cell is because the protoplasm sex change is solidified, therefore cell sap can not maintain in the vacuole,, behind neutral red staining, do not produce the vacuole coloring phenomenon, opposite, the cation of dimethyl diaminophenazine chloride but with protoplasm and the cell nucleus of certain negative electrical charge is combined, and makes protoplasm and cell nucleus dyeing.Dimethyl diaminophenazine chloride is nontoxic, often does the dyestuff of live body dyeing, is used for dying protozoa and shows the inclusion etc. of living cells in the animal vegetable tissue.Old neutral red aqueous solution of a specified duration is as the common dyes that shows the Neil body.Therefore, carry out dimethyl diaminophenazine chloride as vital stain distinguishing the research of parent freshwater shrimp and Qiu Fanmiao, to reduce inbred probability, to reach the protection of freshwater shrimp germ plasm resource, realize the lasting of shrimp culture industry, sound development plays an important role.
Dimethyl diaminophenazine chloride is the alkalescent dyestuff, takes on a red color Powdered, can water-soluble (solvability 4%) and alcohol (solvability 1.8%).Present yellow in its alkaline solution, in strong alkali solution, be blue, and in weakly acidic solution, take on a red color, so can be used as indicator.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, a kind of freshwater shrimp dimethyl diaminophenazine chloride vital staining method is provided, and the suitableeest dyeing time and dyeing concentration is provided, to reach the not vigor of unhealthful freshwater shrimp of stable dyeing.Utilize the suitableeest dyeing time of dimethyl diaminophenazine chloride provided by the invention and dyeing concentration can distinguish freshwater shrimp parent and Qiu Fanmiao; provide fundamental basis to realizing the freshwater shrimp family selective breeding; reduce inbred probability; to reach the protection of freshwater shrimp germ plasm resource; realize continuing of shrimp culture industry; develop in a healthy way, be freshwater shrimp stock breeding based theoretical.
According to technical scheme provided by the invention, numerous differentiation of seedling method of a kind of parent freshwater shrimp and autumn, step is:
(1) configuration of neutral red solution: get the dimethyl diaminophenazine chloride powder, pour test cylinder (jar) into, inject while stirring running water, waterflood injection rate is 40 ~ 60 dm
3/ h, mixing speed is 100 ~ 200r/min, being mixed with Zhong Hong ︰ water quality ratio is the neutral red solution of 1 ︰ 4000 ~ 7000;
(2) freshwater shrimp is raised: get healthy freshwater shrimp and in the neutral red solution of step (1) configuration, raise 6h ~ 5d, and continuous oxygenation and feeding in the feeding process, every day, feeding was twice.
Waterflood injection rate is 50 dm in the step (1)
3/ h, mixing speed is 150 r/min.
Described Zhong Hong ︰ water quality ratio is 1 ︰ 4000 ~ 5000.
Described Zhong Hong ︰ water quality ratio is 1 ︰ 4000,1 ︰ 4500 or 1 ︰ 5000.
The described feeding time of step (2) is 5d, and dissolved oxygen amount is 5 ~ 7 mg/L.
Described dissolved oxygen amount is 6.5 mg/L.
Utilize dimethyl diaminophenazine chloride to see examination as to substances reference 1 as the proof of vital staining agent safety.
The present invention has following advantage: the present invention is take dimethyl diaminophenazine chloride as material; the suitableeest dye strength and the suitableeest dyeing time of dimethyl diaminophenazine chloride freshwater shrimp vital staining have successfully been found out; obtained preferably freshwater shrimp vital staining method of a kind of effect; can be used for distinguishing freshwater shrimp parent and Qiu Fanmiao; reduce inbred probability; to reach the protection of freshwater shrimp germ plasm resource; realize continuing of shrimp culture industry; develop in a healthy way, for freshwater shrimp stock breeding based theoretical and promote the application of other vital stains in the freshwater shrimp vital staining.
Embodiment
The invention discloses the suitableeest dye strength and the dyeing time of dimethyl diaminophenazine chloride freshwater shrimp vital staining.Those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is, all similarly replace and change apparent to those skilled in the art, and they all are deemed to be included in the present invention.Method of the present invention and application are described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described within not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
Embodiment 1
(1) configuration of neutral red solution: get the dimethyl diaminophenazine chloride powder, pour test cylinder (jar) into, inject while stirring running water, waterflood injection rate is 40 ~ 60 dm
3/ h, mixing speed is 100 ~ 200r/min; Preparation Zhong Hong ︰ water quality ratio is that the dimethyl diaminophenazine chloride dyestuff of 1:4000,1:4500,1:5000,1:6000 and 1:7000 concentration is with the dimethyl diaminophenazine chloride dye strength of definite the best;
(2) freshwater shrimp is raised: get healthy freshwater shrimp and raise dyeing in the neutral red solution of step (1) configuration, still constantly oxygenation and feeding in dyeing course; The freshwater shrimp dimethyl diaminophenazine chloride vital staining time of different time gradient is 6 hours, 1 day, 2 days, 3 days and 5 days; Continuous oxygenation and feeding (the molten amount of itching is 6.5mg/L) in the feeding process, every day, feeding was 2 times;
The experiment freshwater shrimp was dyeed 5 days in the dimethyl diaminophenazine chloride dyestuff of 1:4000,1:4500 and 1:5000 concentration, and the experiment freshwater shrimp has preferably Color, and whole body is obvious redness; Pull out in the rear clear water raise 2 after, redness is still high-visible, and experiment freshwater shrimp vigor is better, can be used for distinguishing the research of parent freshwater shrimp and Qiu Fanmiao; But it is better to test comparatively speaking the Color of freshwater shrimp in the 1:4500 dye strength.The experiment freshwater shrimp of other dyeing time sections the phenomenon of fading all occurs raise a period of time in clear water after, until with common freshwater shrimp without significant difference till, can't be applied to distinguish the research of parent freshwater shrimp and Qiu Fanmiao.
All experiment freshwater shrimps of in the dimethyl diaminophenazine chloride dyestuff of 1:6000 and 1:7000 concentration, dyeing, dyeing time length no matter, experiment the phenomenon of fading all occurs after a period of time in clear water, until with common freshwater shrimp without significant difference till, can't be applied to distinguish the research of parent freshwater shrimp and Qiu Fanmiao.
Gu the suitableeest freshwater shrimp neutral red staining dye strength is 1:4000,1:4500 and 1:5000 also can be used for parent freshwater shrimp and numerous differentiation of seedling of autumn; The suitableeest dimethyl diaminophenazine chloride dyeing time is 5 days.The experiment freshwater shrimp is dyeed in this dye strength and dyeing time and can obtain comparatively stable Color; the available research of carrying out differentiation parent freshwater shrimp and Qiu Fanmiao; to with reduce inbred probability; to reach the protection of freshwater shrimp germ plasm resource; realize continuing of shrimp culture industry, sound development plays an important role.
Embodiment 2
(1) configuration of neutral red solution: get the dimethyl diaminophenazine chloride powder, pour test cylinder (jar) into, inject while stirring running water, waterflood injection rate is 40 ~ 60 dm
3/ h, mixing speed is 100 ~ 200r/min, is mixed with Zhong Hong ︰ water quality than being 1:4000,1; 4500, the dimethyl diaminophenazine chloride dyestuff of 1:5000,1:6000 and 1:7000 concentration.
(2) freshwater shrimp is raised: get healthy freshwater shrimp and in the neutral red solution of step (1) configuration, raise, and continuous oxygenation and feeding in the feeding process, every day, feeding was twice.Freshwater shrimp dimethyl diaminophenazine chloride vital staining: the freshwater shrimp of picking health, raise in the dimethyl diaminophenazine chloride dye liquor of each formulated variable concentrations gradient (15 of each concentration gradients).
The Coloration experiment of freshwater shrimp different time gradient: respectively at 6 hours, 1 day, 2 days, 3 shrimps were so taken out also from the dimethyl diaminophenazine chloride of each variable concentrations gradient in 3 days and 5 days, raise in clear water and oxygenation (the molten amount of itching is 6.5mg/L).Change water and the screw of throwing something and feeding every day, standard is that freshwater shrimp is not starved, and records the health of vital staining freshwater shrimp every day, casts off a skin and fades phenomenon.
The suitableeest dimethyl diaminophenazine chloride vital staining concentration of freshwater shrimp is 1:4000, and the dimethyl diaminophenazine chloride dye strength of 1:4500 and 1:5000 also can obtain comparatively stable Color; The suitableeest dimethyl diaminophenazine chloride vital staining time is 5 days.The experiment freshwater shrimp is dyeed in this dye strength and dyeing time and can obtain comparatively stable Color; the available research of carrying out differentiation parent freshwater shrimp and Qiu Fanmiao; to with reduce inbred probability; to reach the protection of freshwater shrimp germ plasm resource; realize continuing of shrimp culture industry, sound development plays an important role.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
3 one kinds of parent freshwater shrimps of embodiment and numerous differentiation of seedling method of autumn
(1) configuration of neutral red solution: get the dimethyl diaminophenazine chloride powder, pour test cylinder (jar) into, inject while stirring running water, waterflood injection rate is 50 dm
3/ h, mixing speed is 150r/min, is mixed with Zhong Hong ︰ water quality than being the neutral red solution of 1 ︰ 4000;
(2) freshwater shrimp is raised: get healthy freshwater shrimp and in the neutral red solution of step (1) configuration, raise 5d, and continuous oxygenation and feeding in the feeding process, every day, feeding was twice; Dissolved oxygen amount is 6.5 mg/L.
Claims (6)
1. a parent freshwater shrimp and numerous differentiation of seedling method of autumn is characterized in that step is:
(1) configuration of neutral red solution: get the dimethyl diaminophenazine chloride powder, pour test cylinder (jar) into, inject while stirring running water, waterflood injection rate is 40 ~ 60 dm
3/ h, mixing speed is 100 ~ 200r/min, being mixed with Zhong Hong ︰ water quality ratio is the neutral red solution of 1 ︰ 4000 ~ 7000;
(2) freshwater shrimp is raised: get healthy freshwater shrimp and in the neutral red solution of step (1) configuration, raise 6h ~ 5d, and continuous oxygenation and feeding in the feeding process, every day, feeding was twice.
2. numerous differentiation of seedling method of parent freshwater shrimp and autumn as claimed in claim 1, it is characterized in that: waterflood injection rate is 50 dm in the step (1)
3/ h, mixing speed is 150 r/min.
3. such as parent freshwater shrimp and numerous differentiation of seedling method of autumn as described in one of claim 1 ~ 2, it is characterized in that: described Zhong Hong ︰ water quality ratio is 1 ︰ 4000 ~ 5000.
4. numerous differentiation of seedling method of parent freshwater shrimp and autumn as claimed in claim 3, it is characterized in that: described Zhong Hong ︰ water quality ratio is 1 ︰ 4000,1 ︰ 4500 or 1 ︰ 5000.
5. such as parent freshwater shrimp and numerous differentiation of seedling method of autumn as described in one of claim 1 ~ 4, it is characterized in that: the described feeding time of step (2) is 5d, and dissolved oxygen amount is 5 ~ 7 mg/L.
6. numerous differentiation of seedling method of parent freshwater shrimp and autumn as claimed in claim 5, it is characterized in that: described dissolved oxygen amount is 6.5 mg/L.
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| CN2012104327511A CN102919171A (en) | 2012-11-03 | 2012-11-03 | Method for distinguishing parent freshwater shrimps from autumn breeding seedlings |
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| CN2012104327511A CN102919171A (en) | 2012-11-03 | 2012-11-03 | Method for distinguishing parent freshwater shrimps from autumn breeding seedlings |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250661A (en) * | 2013-04-26 | 2013-08-21 | 中国水产科学研究院黄海水产研究所 | Aurelia vital staining method |
Citations (4)
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|---|---|---|---|---|
| US3673986A (en) * | 1970-11-12 | 1972-07-04 | Harold N Braunhut | Method and materials used for hatching brine shrimp |
| CN101569295A (en) * | 2009-06-04 | 2009-11-04 | 浙江省淡水水产研究所 | Method for breeding individually-labeled giant freshwater shrimps and labeled lattices for cultivation |
| CN101773086A (en) * | 2010-02-09 | 2010-07-14 | 中国科学院南海海洋研究所 | Method for labeling shrimps and shrimp label |
| CN102687692A (en) * | 2012-06-04 | 2012-09-26 | 中国水产科学研究院黄海水产研究所 | Release method capable of accurately tracing released Chinese prawns |
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2012
- 2012-11-03 CN CN2012104327511A patent/CN102919171A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3673986A (en) * | 1970-11-12 | 1972-07-04 | Harold N Braunhut | Method and materials used for hatching brine shrimp |
| CN101569295A (en) * | 2009-06-04 | 2009-11-04 | 浙江省淡水水产研究所 | Method for breeding individually-labeled giant freshwater shrimps and labeled lattices for cultivation |
| CN101773086A (en) * | 2010-02-09 | 2010-07-14 | 中国科学院南海海洋研究所 | Method for labeling shrimps and shrimp label |
| CN102687692A (en) * | 2012-06-04 | 2012-09-26 | 中国水产科学研究院黄海水产研究所 | Release method capable of accurately tracing released Chinese prawns |
Non-Patent Citations (3)
| Title |
|---|
| RICHARD A. NEAL ET AL: "《FAO Fisheries Reports》", 31 December 1968, FISH.PUB., article "Methods of marking shrimp", pages: E/70 - 4 * |
| 张堂林等: "鱼类标志技术的研究进展", 《中国水产科学》, vol. 10, no. 3, 30 June 2003 (2003-06-30), pages 246 - 253 * |
| 林元华: "海洋生物标志放流技术的研究状况", 《海洋科学》, vol. 9, no. 5, 30 September 1985 (1985-09-30), pages 54 - 58 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250661A (en) * | 2013-04-26 | 2013-08-21 | 中国水产科学研究院黄海水产研究所 | Aurelia vital staining method |
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Application publication date: 20130213 |