CN102901705A - System and method for detecting hemoglobin concentration based on single chip - Google Patents
System and method for detecting hemoglobin concentration based on single chip Download PDFInfo
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- CN102901705A CN102901705A CN2012103788402A CN201210378840A CN102901705A CN 102901705 A CN102901705 A CN 102901705A CN 2012103788402 A CN2012103788402 A CN 2012103788402A CN 201210378840 A CN201210378840 A CN 201210378840A CN 102901705 A CN102901705 A CN 102901705A
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Abstract
The invention discloses a system and a method for detecting hemoglobin concentration based on a single chip. The system comprises a light source module, a colorimetric pool, a photodiode, an optical signal processing module and the single chip, wherein the light source module comprises a D/A (digital/analogue) converter, a voltage-controlled constant current source and an LED (light-emitting diode); the optical signal processing module comprises a photoelectric converter, an A/D (analogue/digital) converter and a human-computer interaction interface; the single chip is connected with the D/A converter; the D/A converter is connected with the voltage-controlled constant current source; the voltage-controlled constant current source is connected with the LED; the light emitted by the LED is received by the photodiode after transmitting the colorimetric pool; the photodiode is connected with the photoelectric converter; the photoelectric converter is connected with the A/D converter; the A/D converter is connected with the single chip; and the human-computer interaction interface is connected with the single chip. The system has the advantage that the voltage-controlled constant current source is used for controlling a light source, so that a measurement is extremely accurate while a reliable guarantee is provided for post-signal conversion and voltage stabilization.
Description
Technical field
The present invention relates to a kind of hemoglobin concentration detection system and method, especially relate to a kind of SCM Based hemoglobin concentration detection system and method.
Background technology
The Lambert-Beer law is a philosophy in the absorptiometry.It refers to that chromophoric absorbance is linear change along with the variation of sample cell light path and chromophore concentration in transparent solvent.This law is the simplified characterization to Maxwell's far field equation of the mutual relationship of describing light and material.Spectrophotometric method is by measuring measured matter Optical Absorption degree in certain wave strong point or certain wavelength coverage, this material being carried out the method for qualitative and quantitative analysis.Absorbance refers to that wavelength is the light of λ passes through the projection light intensity rate behind solution or the material by the incident intensity before solution or a certain material and this light logarithm.
Traditional hemoglobin concentration detection method has single wavelength spectra photometric method, dual wave length spectrophotometry degree method and derivative spectra photometric method.The double wave regular way mostly adopts spectroscope to obtain the monochromatic light of two kinds of wavelength, the optical texture relative complex, and practical application is less.Derivative scene degree rule needs the light source of more different-waveband to measure many group solution concentrations, and adopts the multiple light courcess system must cause the high complexity of system.Single wavelength spectra photometric method is one of at present common hemoglobin concentration measuring method, and the needed optical system of this method is relatively simple, and accuracy meets general clinical measurement requirement.
In the last few years, hemoglobin concentration detection method based on ARM or FPGA emerges, and also obtained certain effect in the actual use, but its many employing open loop controls, its shortcoming is the Stability and veracity that can't guarantee hemoglobin concentration, another shortcoming is the hardware configuration meeting more complicated of system, debug difficulties during practical application, and cost of development is high.
Summary of the invention
Technical matters to be solved by this invention provides a kind of the detection accurately and the SCM Based hemoglobin concentration detection statistics system and method for good stability.
The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of SCM Based hemoglobin concentration detection statistics system, comprise light source module, colorimetric pool, photodiode, light signal processing module and single-chip microcomputer, light source module comprises D/A converter, voltage controlled current source and LED light emitting diode, described light signal processing module comprises photoelectric commutator, A/D converter and human-computer interaction interface, described single-chip microcomputer is connected with D/A converter, D/A converter is connected with voltage controlled current source, voltage controlled current source is connected with the LED light emitting diode, behind the light transmission colorimetric pool that light emitting diode sends, received by described photodiode, described photodiode is connected with described photoelectric commutator, described photoelectric commutator is connected with A/D converter, A/D converter is connected with described single-chip microcomputer, and human-computer interaction interface is connected with single-chip microcomputer.
Photoelectric switching circuit comprises current/voltage-converted circuit and voltage inversion circuit, and described photodiode is connected with the current/voltage-converted circuit, and the current/voltage-converted circuit is connected with the voltage reversal circuit, and the voltage inversion circuit is connected with A/D converter.
A kind of SCM Based hemoglobin concentration detection statistics method is characterized in that comprising the steps:
Step 1: by the Single-chip Controlling D/A converter, again with the output voltage values of D/A converter to controlled constant-current source circuit, obtain stable electric current to light emitting diode, obtain stable light source;
Step 2: put into dilution in colorimetric pool, the dilution in the light transmission colorimetric pool of light emitting diode obtains current i through photodiode
0, then obtain magnitude of voltage U through the current/voltage-converted circuit
I0=-i
0* R, R is the transfer resistance in the current/voltage-converted circuit, the magnitude of voltage U that obtains
I0Be input to A/D converter through after the negative circuit again, the output voltage values U of last A/D converter
0=-U
I0, photodiode is to the susceptibility S=i of light
0/
I 0, and get final product
I 0=-U
0/ SR;
Step 3: setting voltage reference value, calculating voltage reference value and the magnitude of voltage U that collects
0Difference e (k), with difference e (k) substitution formula:
, wherein u (k) is the value that the k time control calculates constantly, K
PBe scale-up factor, K
iBe integral coefficient, again with u (k) substitution formula: U (k)=U (k-1)+u (k) obtains feeding back output valve, then will feed back output valve U (k) and be input to D/A converter, output valve with D/A converter is input to controlled constant-current source circuit at last, adjust the light intensity of light emitting diode, if magnitude of voltage U
0Also do not reach voltage reference value, then repeat this step, until magnitude of voltage U
0Equal voltage reference value;
Step 4: put into tested blood sample in colorimetric pool, the blood sample in the light transmission colorimetric pool of light emitting diode obtains current i through photodiode
1, then obtain magnitude of voltage U through the current/voltage-converted circuit
I1=-i
1* R, R is the transfer resistance in the current/voltage-converted circuit, the magnitude of voltage that obtains is input to A/D converter through after the negative circuit again, the output voltage values U of last A/D converter
1=-U
I1, photodiode is to the susceptibility S=i of light
1/
I 1,
I 1=-U
1/ SR;
Step 5: step 2 is obtained
I 0 Obtain with step 4
I 1 The substitution formula
, namely obtain the value of hemoglobin concentration, wherein C is hemoglobin concentration, and K is absorptivity, and L is colorimetric pool thickness.
Compare with modern technologies, advantage of the present invention is to adopt voltage controlled current source control light source, provides reliable assurance also to make measurement more accurate simultaneously to the stable of the conversion of rear class signal and voltage.The modules circuit that the present invention adopts is simple, and cost is lower.The present invention adopts closed-loop control, can effectively reduce extraneous factor to the impact of hemoglobin concentration stability.The nonlinearity erron of actual measurement hemoglobin concentration can be controlled in 2.42%.
Description of drawings
Fig. 1 is general structure synoptic diagram of the present invention;
Fig. 2 is photoelectric switching circuit structural representation of the present invention;
Fig. 3 process flow diagram of the present invention;
Fig. 4 is the process flow diagram of closed-loop control of the present invention;
Fig. 5 is the hemoglobin concentration stability curve figure that the present invention surveys;
Fig. 6 is the hemoglobin concentration linear diagram that the present invention surveys;
Embodiment
Embodiment is described in further detail the present invention below in conjunction with accompanying drawing.
As shown in Figure 1, the hemoglobin concentration detection system mainly comprises: comprise light source module, colorimetric pool and light signal processing module, described light source module comprises D/A converter 2, controlled constant-current source circuit 3, LED light emitting diode 4.Described D/A converter 2 is connected with described controlled constant-current source circuit 3 with described single-chip microcomputer 1, and described controlled constant-current source circuit 3 is connected with described LED light emitting diode 4.The described colorimetric pool 5 of the light transmission that described LED light emitting diode 4 sends, described light signal processing module comprises photoelectric switching circuit 7, A/D converter 10, human-computer interaction interface 11.Described photoelectric switching circuit 7 is connected with A/D converter 8, it is characterized in that photoelectric switching circuit comprises current/voltage-converted circuit 8 and negative circuit 9.Described current/voltage-converted circuit 8 is connected with negative circuit 9.Described A/D converter 10 is connected with single-chip microcomputer 1.Described human-computer interaction interface 11 is connected with single-chip microcomputer 1.
A kind of SCM Based hemoglobin concentration detection method, specifically comprise the steps step 1: by Single-chip Controlling D/A converter 2, again with the output voltage values of D/A converter 2 to controlled constant-current source circuit 3, obtain stable electric current to light emitting diode 4, obtain stable light source.
Step 2: put into dilution in colorimetric pool 5, the dilution in the light transmission colorimetric pool 5 of light emitting diode 4 obtains current i through photodiode 6
0, then obtain magnitude of voltage U through current/voltage-converted circuit 8
I0=-i
0* R, R are the transfer resistance in the current/voltage-converted circuit.The magnitude of voltage that obtains is input to A/D converter 10 through after the negative circuit 9 again, the output voltage values U of last A/D converter 10
0=-U
I0, the susceptibility S=i of 6 pairs of light of photodiode among the present invention
0/
I 0So,
I 0=-U
0/ SR.Fig. 2 is the photoelectric switching circuit structural representation.
Step 3: setting voltage reference value, calculating voltage reference value and the magnitude of voltage U that collects
0Difference e (k), with difference e (k) substitution formula:
, wherein u (k) is the value that the k time control calculates constantly, K
PBe scale-up factor, K
iBe integral coefficient.Again with u (k) substitution formula: U (k)=U (k-1)+u (k) obtains feeding back output valve, then will feed back output valve U (k) and be input to D/A converter, output valve with D/A converter is input to controlled constant-current source circuit at last, adjust the light intensity of light emitting diode, if magnitude of voltage U
0Also do not reach voltage reference value, then repeat this step, until magnitude of voltage U
0Equal voltage reference value.
Step 4: put into tested blood sample in colorimetric pool 5, the light transmission colorimetric pool 5 interior blood samples of light emitting diode 4 obtain corresponding current i through photodiode 4
1, then obtain magnitude of voltage U through current/voltage-converted circuit 8
I1=-i
1* R, R are the transfer resistance in the current/voltage-converted circuit.The magnitude of voltage that obtains is input to A/D converter 10 through after the negative circuit 9 again, the output voltage values U of last A/D converter 10
1=-U
I1, photodiode is to the susceptibility S=i of light among the present invention
1/
I 1,
I 1=-U
1/ SR.
Step 5: step 2 is obtained
I 0 Obtain with step 4
I 1 The substitution formula
, wherein C is hemoglobin concentration, and K is absorptivity, and L is colorimetric pool thickness.
As shown in Figure 3, briefly described the process flow diagram of this statistical method among the figure.This statistical method is carried out with step 1 → step 2 → step 3 → step 4 → step 5 order.
What describe among the figure as shown in Figure 4, is the process flow diagram of the closed-loop control of step 3 in this statistical method.The magnitude of voltage U that collects in reference voltage level and the step 1
0Between difference as the input of closed-loop control, the output valve U of closed-loop control (k)=U (k-1)+u (k).When if controller is output as U (k), the magnitude of voltage U that collects in the step 1
0Also do not reach reference value, then continue the closed-loop control of step 3, until the magnitude of voltage U that step 1 collects
0Equal reference value.
Fig. 5 and Fig. 6 are respectively hemoglobin concentration stability curve figure and the linear diagram that the present invention surveys because the stability of hemoglobin concentration and linear with
Identical.In stability test, be in the situation of 13uL adding blood sample concentration, continuous coverage 10 times, the each blood sample of surveying of record
Value, drawing its mean value is 0.299, variance is 1.6 * 10
-6In the online property testing, take 2uL as the interval concentration is divided into 6 groups, surveys 5 times to get mean value for every group, this moment, nonlinearity erron was 2.42%FS.
Claims (3)
1. SCM Based hemoglobin concentration detection system, it is characterized in that comprising light source module, colorimetric pool, photodiode, the light signal processing module, single-chip microcomputer and human-computer interaction interface, light source module comprises D/A converter, voltage controlled current source and LED light emitting diode, described light signal processing module comprises photoelectric commutator, A/D converter, described single-chip microcomputer is connected with D/A converter, D/A converter is connected with voltage controlled current source, voltage controlled current source is connected with the LED light emitting diode, behind the light transmission colorimetric pool that light emitting diode sends, received by described photodiode, described photodiode is connected with described photoelectric commutator, described photoelectric commutator is connected with A/D converter, A/D converter is connected with described single-chip microcomputer, and human-computer interaction interface is connected with single-chip microcomputer.
2. a kind of SCM Based hemoglobin concentration detection system according to claim 1, it is characterized in that photoelectric switching circuit comprises current/voltage-converted circuit and voltage inversion circuit, described photodiode is connected with the current/voltage-converted circuit, the current/voltage-converted circuit is connected with the voltage reversal circuit, and the voltage inversion circuit is connected with A/D converter.
3. a SCM Based hemoglobin concentration detection method is characterized in that comprising the steps:
Step 1: by the Single-chip Controlling D/A converter, again with the output voltage values of D/A converter to controlled constant-current source circuit, obtain stable electric current to light emitting diode, obtain stable light source;
Step 2: put into dilution in colorimetric pool, the dilution in the light transmission colorimetric pool of light emitting diode obtains current i through photodiode
0, then obtain magnitude of voltage U through the current/voltage-converted circuit
I0=-i
0* R, R is the transfer resistance in the current/voltage-converted circuit, the magnitude of voltage U that obtains
I0Be input to A/D converter through after the negative circuit again, the output voltage values U of last A/D converter
0=-U
I0, photodiode is to the susceptibility S=i of light
0/
I 0, and get final product
I 0=-U
0/ SR;
Step 3: setting voltage reference value, calculating voltage reference value and the magnitude of voltage U that collects
0Difference e (k), with difference e (k) substitution formula:
, wherein u (k) is the value that the k time control calculates constantly, K
PBe scale-up factor, K
iBe integral coefficient, again with u (k) substitution formula: U (k)=U (k-1)+u (k) obtains feeding back output valve, then will feed back output valve U (k) and be input to D/A converter, output valve with D/A converter is input to controlled constant-current source circuit at last, adjust the light intensity of light emitting diode, if magnitude of voltage U
0Also do not reach voltage reference value, then repeat this step, until magnitude of voltage U
0Equal voltage reference value;
Step 4: put into tested blood sample in colorimetric pool, the blood sample in the light transmission colorimetric pool of light emitting diode obtains current i through photodiode
1, then obtain magnitude of voltage U through the current/voltage-converted circuit
I1=-i
1* R, R is the transfer resistance in the current/voltage-converted circuit, the magnitude of voltage that obtains is input to A/D converter through after the negative circuit again, the output voltage values U of last A/D converter
1=-U
I1, photodiode is to the susceptibility S=i of light
1/
I 1,
I 1=-U
1/ SR;
Step 5: step 2 is obtained
I 0 Obtain with step 4
I 1 The substitution formula
, namely obtain the value of hemoglobin concentration, wherein C is hemoglobin concentration, and K is absorptivity, and L is colorimetric pool thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210378840.2A CN102901705B (en) | 2012-10-08 | 2012-10-08 | System and method for detecting hemoglobin concentration based on single chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210378840.2A CN102901705B (en) | 2012-10-08 | 2012-10-08 | System and method for detecting hemoglobin concentration based on single chip |
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|---|---|
| CN102901705A true CN102901705A (en) | 2013-01-30 |
| CN102901705B CN102901705B (en) | 2015-04-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725323A (en) * | 2016-12-22 | 2017-05-31 | 中国科学院苏州生物医学工程技术研究所 | Wearable biological signal collecting device |
| CN111323381A (en) * | 2020-04-14 | 2020-06-23 | 深圳联开生物医疗科技有限公司 | Background voltage self-adaption method, measuring method, cell analyzer and storage medium |
| CN113777076A (en) * | 2021-08-30 | 2021-12-10 | 四川南格尔生物科技有限公司 | Sensor and method for online real-time concentration monitoring |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725323A (en) * | 2016-12-22 | 2017-05-31 | 中国科学院苏州生物医学工程技术研究所 | Wearable biological signal collecting device |
| CN111323381A (en) * | 2020-04-14 | 2020-06-23 | 深圳联开生物医疗科技有限公司 | Background voltage self-adaption method, measuring method, cell analyzer and storage medium |
| CN113777076A (en) * | 2021-08-30 | 2021-12-10 | 四川南格尔生物科技有限公司 | Sensor and method for online real-time concentration monitoring |
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| CN102901705B (en) | 2015-04-29 |
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