CN2069114U

CN2069114U - Determination apparatus for determining hemoglobin

Info

Publication number: CN2069114U
Application number: CN 90207851
Authority: CN
Inventors: 戈鹤川; 李经正; 戈鹤山; 汪华
Original assignee: Individual
Current assignee: Individual
Priority date: 1990-06-04
Filing date: 1990-06-04
Publication date: 1991-01-09

Abstract

The utility model belongs to a clinical biochemical detection instrument for detecting the concentration of hemoglobin and the number of red blood cells, comprising a photoelectric detection system and a control system. Two kinds of flashes with different wavelengths, which are obtained by a two-color light-emitting diode, are used for replacing a single wavelength single light beam which is used at present, the operation of blank correction is avoided, and automatic recognition, memory and calibration can be realized by electronic devices of a single-chip computer, etc., so that the purposes of fast measurement speed, small size and low power consumption can be obtained. Accordingly, the instrument is suitable for extensive base units and the working needs of general surveys.

Description

Determination apparatus for determining hemoglobin

The utility model relates to a kind of clinical biochemical index that is used for--and the blood testing instrument that hemoglobin concentration and RBC number are measured, it is made up of photodetector system and control system.

Chang Yong hemoglobin determinator device great majority are single wavelength single beam photometer or tintmeter clinically.According to law of Beer:

A＝lgI ₀／I＝KLC

Wherein A is by being surveyed solution absorbency; I ₀Be incident intensity, the blank value when ie in solution does not have absorption to light; I is a transmitted light intensity, and ie in solution has the value that sees through when absorbing to light; K by the molar absorption coefficient of survey material; L is the cuvette light path; C by the concentration of survey solution.By photometric measurement, utilize the demarcation of standard solution, thereby make the concentration of the solution of surveying.As the BS622 type chromocytometer (consulting its instructions) of Beijing biochemical instrument factory production and the blood red born of the same parents' analyser of XF503 type (consulting its instructions) of Shanghai medical analytical instrument factory production.This quasi-instrument uses tungsten light source usually, or other incandescent lamp source.Just can make concentration owing to must measure blank value and see through value, so higher to the stability requirement of light source and detection system.Therefore must cause complex structure, volume is bigger, and the power consumption height requires long pre-heat-stable time before the work, and working environment is also had higher requirement.Be unfavorable for that like this carry at the scene and measurement fast.

The purpose of this utility model is to utilize the dual wavelength determination method to avoid the principle of blank value measurement, and it is fast to design a kind of finding speed, and the instrument volume is little, hemoglobin concentration that low in energy consumption and automatic control degree is high and RBC number determining instrument.

At first dual wavelength being measured ratio juris sketches in following:

When the light that adopts two kinds of different wave lengths shines solution, then same solution (is established its wavelength and is respectively λ two different wavelengths of light ₁, λ ₂) absorbance that produces is A(λ _{1 λ}), A(λ ₂), promptly

A（λ ₁）＝lg｛I ₀（λ ₂）／I（λ ₁）｝＝K（λ ₁）LC

A（λ ₂）＝lg｛I ₀（λ ₂）／I（λ ₁）｝＝K（λ ₂）LC

Regulate the relevant parameter of instrument and make I ₀(λ ₁)=I ₀(λ ₂), promptly be called school zero.Get the poor of two wavelength absorption degree, even △ A=A(is λ ₂)-A(λ ₁).Have after the arrangement:

△A＝lg｛I（λ ₁）／I（λ ₂）｝＝｛K（λ ₂）－K（λ ₁）｝LC

This formula shows that △ A still is directly proportional with the concentration of survey material, and all the other are constant, so can release concentration by △ A.

This analyzer is made up of photodetector system and control system two parts.It is characterized in that according to above-mentioned principle the side as the cavity of the measuring box of photodetector system is provided with a double color luminotron, the flash of light that is used to produce two kinds of wavelength, a photoelectric cell measuring its light intensity correspondingly thereto is arranged on the opposite side of cavity.The measuring box top is provided with infrared switch to pipe and shadow shield, is used to control automatic zero adjustment.Cuvette is placed in the box chamber, is located by positioning key.Described control system comprises single chip microcomputer, A-D converter (ADC), digital-to-analog converter (DAC), programmable amplifier, power-supply controller of electric, display panel, keyboard and maintenance power supply.Whole arrangements of components constitutes a whole instrument in a cabinet.

Description of drawings:

Fig. 1 is the structural drawing of measuring box.Wherein 1 is that cavity, 2 is that dichromatic LED, 3 is that photoelectric cell, 4 is that infrared switch is that shadow shield, 6 is a positioning key to pipe, 5.

Fig. 2 is the block scheme of control system, and wherein 7 is that single chip microcomputer, 8 is that A-D converter, 9 is that digital-to-analog converter, 10 is that programmable amplifier, 11 is that power-supply controller of electric, 12 is that display panel, 13 is that keyboard, 14 is that maintenance power supply, 15 is a measuring box.

Structure and working routine to instrument is further described in conjunction with the accompanying drawings.

Measuring box 15 as detection system is key components of this analyzer.It is made of cavity 1 measures the darkroom, and its material adopts plastic plate or sheet metal, and thickness requirement is not strict, satisfies certain intensity and gets final product.On a side of the cavity 1 of measuring box 15, a dichromatic LED 2 is set, the flash of light that is used to produce two kinds of wavelength.On its relative side, a photoelectric cell 3 of measuring light intensity is set.Above cavity 1, be provided with infrared switch to managing 4 and shadow shield 5, be used to control automatic zero adjustment.Infrared switch is electronic switches to managing 4, and when not inserting cuvette in the box, shadow shield 5 is closed, and infrared switch is unimpeded to managing 4 light path, and switch open enters automatic zero adjustment.When inserting cuvette in the box and measure, shadow shield 5 is open, and infrared switch is obstructed to managing 4 light path, closes automatic zero adjustment, and positioning key 6 is located to colorimetric blood.

Contrast colors light emitting diode 4 produces the control of dual wavelength flash of light, the execution of automatic zero adjustment and the memory of the parameter that the instrument recognition obtains, and all realizes by control system.Control system comprises single chip microcomputer 7, A-D converter 8, digital-to-analog converter 9, programmable amplifier 10, power-supply controller of electric 11, display panel 12, keyboard 13 and keeps power supply 14.

Single chip microcomputer 7 provides time signal, lights the light source of two kinds of different wave lengths respectively, alternately produces the flash of light of different wave length, and it also believes number by the amplification of luminous amplifier of electronic switch control change and photoelectric cell current amplifier.A-D converter 8 provides photoelectricity input signal, slope of standard curve corrected value and keyboard input signal.Digital-to-analog converter 9 output automatic zero adjustment signals are sent to constant-current control circuit after programmable amplifier 10 gains adjustment.10 pairs of automatic zero adjustment signals of programmable amplifier and photoelectricity input signal carry out branch time-varying gain control.Control circuit for light source 11 is made up of constant-current circuit and delay-dividing switch circuit, and the constant-current circuit precision is 0.5%, and the adjustment maximal value is 35mA, can cover whole working ranges.Display panel 12 comprises three static liquid crystal displays and three diode displaying, is installed on the instrument panel.Keyboard 13 also is arranged on the panel, and it is discerned and latched by single chip microcomputer 7 to single chip microcomputer 7 input identification signal, school zero-signal and measuring-signals.Keeping power supply 14 is the cuvette identification parameters that keep under holding state in the data memory, and controls the work of school zero system in view of the above.

Utilize this analyzer, adopt photoelectric turbidimetry can measure the red blood cell number.Carry out scattering turbidimetry with the 650-690nm wavelength light and measure, rely on more higher leveled red blood cell count(RBC) instrument that the sample of same turbidity is carried out demarcating after the horizontal survey, by providing measurement result after the microsystem contrast, its precision can satisfy the needs of general clinical detection.

Compare with existing single wavelength single beam detector according to the above-mentioned technical characterictic of this analyzer and to have the following advantages:

1, owing to adopt double-wavelength light source, in actual measurement, removes the blank calibration operation that the single light source instrument all need carry out from, and shorten and give the hot time, therefore save Measuring Time, reach the purpose of Quick Measurement, and improve certainty of measurement;

2, owing to avoid again assorted optical system and high-stability requirement, Gu this is simple in structure, the volume of this analyzer only has 240 * 152 * 58mm³, be part or ten parts of existing instrument, can be made into portable instrument;

3, owing to be provided with automatic identification, memory and calibrating installation, it is higher automatically to control degree, the use that therefore can adapt to different regions, Various Seasonal and replace different cuvettes and Self-made reagent;

4, owing to the maintenance power supply is set and has sleep mode, can save power consumption 3/4ths;

5, owing to replace mechanical switch with electronic switch, can be fit to a large amount of mensuration.

In sum, this analyzer is fit to the needs of the vast grass-roots unit of China and census operations.

Claims

1, a kind of hemoglobin determinator, form by photodetector system and control system, it is characterized in that side as the cavity 1 of the measuring box 15 of described photodetector system, a dichromatic LED 2 that is used to produce the flash of light of two kinds of wavelength is set, a photoelectric cell 3 measuring its light intensity correspondingly thereto is arranged on the another side of cavity 1, the top of measuring box 15 is provided with the infrared switch that is used to control automatic zero adjustment to managing 4 and shadow shield 5, is provided with the positioning key 6 that gives the cuvette location in the box chamber.

2, analyzer according to claim 1 is characterized in that described control system comprises single chip microcomputer 7, A-D converter 8, digital-to-analog converter 9, programmable amplifier 10, power-supply controller of electric 11, display panel 12, keyboard 13 and maintenance power supply 14.

3, analyzer according to claim 1 and 2 is characterized in that described photodetector system and control system are arranged in the cabinet, constitutes a whole instrument, and measuring box is in the box top, and display panel and keyboard are on panel.