CN102884978A - Method for breeding new rice varieties through irradiation - Google Patents

Method for breeding new rice varieties through irradiation Download PDF

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Publication number
CN102884978A
CN102884978A CN 201210271334 CN201210271334A CN102884978A CN 102884978 A CN102884978 A CN 102884978A CN 201210271334 CN201210271334 CN 201210271334 CN 201210271334 A CN201210271334 A CN 201210271334A CN 102884978 A CN102884978 A CN 102884978A
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breeding
seed
new
irradiation
presoaking
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Pending
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CN 201210271334
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Chinese (zh)
Inventor
景德道
盛生兰
钱华飞
林添资
余波
李闯
龚红兵
曾生元
周义文
于苗苗
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JIANGSU FENGYUAN SEED Co Ltd
Zhenjiang Institute of Agricultural Sciences Jiangsu Hilly Area
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JIANGSU FENGYUAN SEED Co Ltd
Zhenjiang Institute of Agricultural Sciences Jiangsu Hilly Area
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Priority to CN 201210271334 priority Critical patent/CN102884978A/en
Publication of CN102884978A publication Critical patent/CN102884978A/en
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Abstract

The invention relates to a method for plant breeding and belongs to the field of genetic breeding. With the improvement of the living standards of people, adjustment of the planting structure and fierce competition in international markets, the problem of how to breed high-yield conventional japonica rice varieties which are high in disease resistance performance and high in taste quality is urgent to be solved. By the method for breeding new rice varieties through irradiation, defects in the prior art can be overcome, and the new rice varieties which are high in quality (high in both exterior quality and taste quality), high in yield and high in disease resistance can be bred in a short time.

Description

A kind of method by the Irradiate breeding new rice variety
Technical field
The present invention relates to the breeding method of a Plants, more specifically to a kind of breeding method by Irradiate breeding high-quality, high yield, disease resisting rice new varieties.
Background technology
Paddy rice is the first crop of China, and the paddy rice of China's cultivation mainly contains two subspecies, i.e. Xian subspecies and round-grained rice subspecies.The Jiangsu Main Cultivation be the round-grained rice subspecies, account for more than 90% of Jiangsu Monitoring of Paddy Rice Plant Area.SOYBEAN IN HIGH-YIELD BREEDING is the important goal of rice breeding always, and China is because populous, and arable area reduces year by year, and the pressure of grain demand strengthens, and SOYBEAN IN HIGH-YIELD BREEDING is put in the first place for a long time always, and the Upgrading of rice quality lags behind.In recent years, along with the raising of people's living standard, the adjustment of pattern of farming and the pressure of competition in the international market, and the impact of factor such as take place frequently of various natural calamity, the quality-improving of paddy rice is more aobvious important.The existing preferably disease-resistant performance of quick breeding how, the high yield conventional japonica rice kind that has again the high-quality food flavor has become rice breeding problem in the urgent need to address.
Summary of the invention
The object of the invention is to solve above-mentioned the deficiencies in the prior art and problem by another kind of approach, a kind of breeding method by the Irradiate breeding new rice variety is provided, thereby cultivate at short notice high-quality (existing good exterior quality have again good Cooking Quality), high yield, disease-resistant new rice variety, make it obtain large scale application.
The applicant is through for many years repetition test, in many hybridization schemes and selection, find, radiation breeding also can reach goal of the invention well rapidly to solve the problem of prior art, and a kind of method by the Irradiate breeding new rice variety of the present invention may further comprise the steps:
A) select the conventional super hybridization rice kind of large-area applications in the current agricultural production or larger application potential, the good new lines (intermediate materials) of Comprehensive Traits are arranged, grain weight is not less than 500g, uses Co 60Carry out irradiation and get M 0
B) about 25 days with M 0Presoaking and germinating is live, and weeding in seedling stage but do not apply fertilizer is executed a small amount of N booting stage fertile, and seed is all gathered in the crops to get M 1
C) with M 1Seed presoaking and germinating sowing, one heart stage an of leaf, executed 10 kilograms of urea/mus, and the interval is with 1 glyphosate spraying processing twice after tri-leaf period, and the individual plant of surviving mixes has kind an of (M in one's care 2);
D) M 2Seed selfing 2-3 selects new rice variety or the new lines better than irradiation material Comprehensive Traits after generation.
According to the described breeding method by the Irradiate breeding new rice variety of claim, it is characterized in that:
(1) the irradiation material is the conventional super hybridization rice kind of large-area applications in the current agricultural production or larger application potential, the good new lines (intermediate materials) of Comprehensive Traits is arranged
(2) contemporary (M 0) plant that after planting forms claims an irradiation generation (M 1) do not eliminate, seed is all gathered in the crops;
(3) with M 1Seed presoaking and germinating sowing, tri-leaf period, the gyphosate solution 200ml with 41% concentration was watered the spraying of 20-30 kg of water, 15 days rear not dead individual plants of transplanting; A fertilising after transplant living, after 5 days again the gyphosate solution 200ml with 41% concentration be watered the spraying of 20-30 kg of water once, the individual plant of surviving mixes has kind an of (M in one's care 2);
(4) M 2After seed selfing 2-3 generation, select new rice variety or the new lines mutant character better than irradiation material Comprehensive Traits usually stable fast, namely experience 3-4 generation and can stablize, thereby breeding cycle is short, breeds new varieties favourable within a short period of time.
Embodiment is as follows:
1) select the conventional super hybridization rice kind of large-area applications in the current agricultural production or have larger application potential, the good new lines (intermediate materials) of Comprehensive Traits to carry out irradiation as the radioactive breeding material, grain weight is not less than 500g, uses Co 60Carry out irradiation and get M 0
2) about 25 days with M 0Presoaking and germinating is live, and thickness of sowing is 40 kg/acres, and weeding in seedling stage but do not apply fertilizer is executed a small amount of N booting stage fertile, and seed is all gathered in the crops to get M 1
3) with M 1Seed presoaking and germinating sowing, thickness of sowing is 80 kg/acres, and one heart stage an of leaf, executed 10 kilograms of urea/mus, and be watered the 20-30 kg of water with the gyphosate solution 200ml of 41% concentration and spray tri-leaf period, 15 days rear not dead individual plants of transplanting; A fertilising after transplant living, after 5 days again the gyphosate solution 200ml with 41% concentration be watered the spraying of 20-30 kg of water once, the individual plant of surviving mixes has kind an of (M in one's care 2);
4) M that mixing is reserved seed for planting 2Then presoaking and germinating sowing 100 grams use up the seedling individual plant transplanting.Only control not diseases prevention of worm, choosing receipts disease is light, Comprehensive Traits good, the individual plant different from the irradiation material is M 3
5) the bad individual plant of indoor superseded exterior quality, remaining M 3Individual plant presoaking and germinating sowing, not diseases prevention of worm is only controlled in every strain transplant planting 48 caves, and choosing receives that disease is light, Comprehensive Traits good, the strain different from the irradiation material, receives to screen according to proterties such as yielding ability, exterior quality, Cooking Qualities afterwards, and elected strain is M 4,
6) be that M4 carries out the new lines approval test with elected stable strain, inoculated identification rice blast and bacterial leaf-blight are carried out in not diseases prevention of whole process heading stage, screen according to proterties such as output, quality, disease-resistant, resistance, and elected strain enters 1 year variety comparative test,
7) variety comparative test, and continue that elected strain is carried out food flavor and taste and attributional analysis, excellent middle select excellent in to keep good food flavor kind;
8) new lines is participated in province or national pilot experiment.
The invention has the beneficial effects as follows:
1. best in quality, food flavor is good, and the rice commodity is good
Breed the new lines physical and chemical index and reach state and issue superior rice standard more than three grades, and food flavor is good.
2. disease resistance is outstanding, and yield stability is good
New lines all has good resistance to bacterial leaf-blight, rice blast and bar banded sclerotial blight, and than fertilizer resistance anti-lodging.
3. yielding ability is good, and the fringe kernel structure is coordinated, and Self-regulation ability is strong
New lines root of hair ability is strong, and tillering ability is strong, and the percentage of earbearing tiller is high, and Self-regulation ability is strong, easily cultivation.
4. plant type is good, ripe lover
Description of drawings
Fig. 1 is breeding method breeding pedigree schematic diagram of the present invention
Embodiment
1) good palatability, the output of selecting me newly to breed on May 8th, 2007 is high, premunition is strong, wide adaptability, precocious late Keng rice lines town 3484 that application potential is very large etc. carry out irradiation as the radioactive breeding material, uses Co 60Irradiation 800 restrains to get M 0
2) on June 5th, 2007 is with M 0Presoaking and germinating is live, sowing 14M 2, weeding in seedling stage but do not apply fertilizer is executed a small amount of N booting stage fertile, and seed is all gathered in the crops to get M 1
3) on May 20th, 2008 is with M 1Seed presoaking and germinating sowing, thickness of sowing is 80 kg/acres, and one heart stage an of leaf, executed 10 kilograms of urea/mus, and be watered the 20-30 kg of water with the gyphosate solution 200ml of 41% concentration and spray tri-leaf period, 15 days rear not dead individual plants of transplanting; A fertilising after transplant living, after 5 days again the gyphosate solution 200ml with 41% concentration be watered the spraying of 20-30 kg of water once, the individual plant of surviving mixes has kind an of (M in one's care 2);
4) M that on May 20th, 2009 mixing is reserved seed for planting 2Then presoaking and germinating sowing 100 grams use up the seedling individual plant transplanting.Only control not diseases prevention of worm, choosing receipts disease is light, Comprehensive Traits good, the individual plant different from the irradiation material is M 3
5) 2010, the bad individual plant of indoor superseded exterior quality, remaining M 3Individual plant presoaking and germinating sowing, not diseases prevention of worm is only controlled in every strain transplant planting 48 caves, and choosing receives that disease is light, Comprehensive Traits good, the strain different from the irradiation material, receives to screen according to proterties such as yielding ability, exterior quality, Cooking Qualities afterwards, and elected strain is M 4,
6) autumn in 2010 adds generation with the better individual plant band of M4 Hainan, and it is M that amount of growth strain large, that plant is neat is received in choosing 5
7) elected stable strain M5 carried out the new lines approval test in 2011, inoculated identification rice blast and bacterial leaf-blight are carried out in not diseases prevention of whole process heading stage, screen according to proterties such as output, quality, disease-resistant, resistance, elected strain enters 1 year variety comparative test
8) new lines variety comparative test in 2012, and continue that elected strain is carried out food flavor and taste and attributional analysis, excellent middle select excellent in to keep good Palatability characteristics;
Concrete breeding is through seeing Table 1.
Table 1: breeding method seed selection process of the present invention
Figure BSA00000757853100041

Claims (5)

1. method breeding method by the Irradiate breeding new rice variety is characterized in that may further comprise the steps:
A) select the conventional super hybridization rice kind of large-area applications in the current agricultural production or larger application potential, the good new lines (intermediate materials) of Comprehensive Traits are arranged, grain weight is not less than 500g, uses Co 60Carry out irradiation and get M0;
B) about 25 days with M 0Presoaking and germinating is live, and weeding in seedling stage but do not apply fertilizer is executed a small amount of N booting stage fertile, and seed is all gathered in the crops to get M 1
C) with M1 seed presoaking and germinating sowing, one heart stage an of leaf, executed 10 kilograms of urea/mus, and the interval is with 1 glyphosate spraying processing twice after tri-leaf period, and the individual plant of surviving mixes has kind an of (M in one's care 2);
D) M 2Seed selfing 2-3 selects new rice variety or the new lines better than irradiation material Comprehensive Traits after generation.
2. the breeding method by the Irradiate breeding new rice variety according to claim 1 is characterized in that the irradiation material is the conventional super hybridization rice kind of large-area applications in the current agricultural production or larger application potential, the good new lines (intermediate materials) of Comprehensive Traits are arranged.
3. the breeding method by the Irradiate breeding new rice variety according to claim 1 is characterized in that (M in the present age 0) plant that after planting forms claims an irradiation generation (M 1) do not eliminate, seed is all gathered in the crops.
4. the breeding method by the Irradiate breeding new rice variety according to claim 1 is characterized in that M 1Seed presoaking and germinating sowing, tri-leaf period, the gyphosate solution 200ml with 41% concentration was watered the spraying of 20-30 kg of water, 15 days rear not dead individual plants of transplanting; A fertilising after transplant living, after 5 days again the gyphosate solution 200ml with 41% concentration be watered the spraying of 20-30 kg of water once, the individual plant of surviving mixes has kind an of (M in one's care 2).
5. the breeding method by the Irradiate breeding new rice variety according to claim 1, its feature is at M 2After seed selfing 2-3 generation, select new rice variety or the new lines mutant character better than irradiation material Comprehensive Traits usually stable fast, namely experience 3-4 generation and can stablize, thereby breeding cycle is short, breeds new varieties favourable within a short period of time.
Embodiment is as follows:
1) select the conventional super hybridization rice kind of large-area applications in the current agricultural production or have larger application potential, the good new lines (intermediate materials) of Comprehensive Traits to carry out irradiation as the radioactive breeding material, grain weight is not less than 500g, uses Co 60Carry out irradiation and get M 0
2) about 25 days with M 0Presoaking and germinating is live, and thickness of sowing is 40 kg/acres, and weeding in seedling stage but do not apply fertilizer is executed a small amount of N booting stage fertile, and seed is all gathered in the crops to get M 1
3) with M 1Seed presoaking and germinating sowing, thickness of sowing is 80 kg/acres, and one heart stage an of leaf, executed 10 kilograms of urea/mus, and be watered the 20-30 kg of water with the gyphosate solution 200ml of 41% concentration and spray tri-leaf period, 15 days rear not dead individual plants of transplanting; A fertilising after transplant living, after 5 days again the gyphosate solution 200ml with 41% concentration be watered the spraying of 20-30 kg of water once, the individual plant of surviving mixes has kind an of (M in one's care 2);
4) M that mixing is reserved seed for planting 2Then presoaking and germinating sowing 100 grams use up the seedling individual plant transplanting.Only control not diseases prevention of worm, choosing receipts disease is light, Comprehensive Traits good, the individual plant different from the irradiation material is M 3
5) the bad individual plant of indoor superseded exterior quality, remaining M 3Individual plant presoaking and germinating sowing, not diseases prevention of worm is only controlled in every strain transplant planting 48 caves, and choosing receives that disease is light, Comprehensive Traits good, the strain different from the irradiation material, receives to screen according to proterties such as yielding ability, exterior quality, Cooking Qualities afterwards, and elected strain is M 4,
6) be M with elected stable strain 4Carry out the new lines approval test, inoculated identification rice blast and bacterial leaf-blight are carried out in not diseases prevention of whole process heading stage, screen according to proterties such as output, quality, disease-resistant, resistance, and elected strain enters 1 year variety comparative test,
7) variety comparative test, and continue that elected strain is carried out food flavor and taste and attributional analysis, excellent middle select excellent in to keep good food flavor kind;
8) new lines is participated in province or national pilot experiment.
CN 201210271334 2012-07-31 2012-07-31 Method for breeding new rice varieties through irradiation Pending CN102884978A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918114A (en) * 2016-01-05 2016-09-07 湖南省核农学与航天育种研究所 Method for improving screening efficiency of target traits in plant radiation mutation breeding
CN109392705A (en) * 2018-11-13 2019-03-01 华南农业大学 The method for creating of weakly sensitivity and high fertility Oryza alta new germ plasm
CN111480571A (en) * 2020-04-26 2020-08-04 上海市农业科学院 Efficient hybrid japonica rice breeding method capable of reducing seed production cost

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918114A (en) * 2016-01-05 2016-09-07 湖南省核农学与航天育种研究所 Method for improving screening efficiency of target traits in plant radiation mutation breeding
CN109392705A (en) * 2018-11-13 2019-03-01 华南农业大学 The method for creating of weakly sensitivity and high fertility Oryza alta new germ plasm
CN111480571A (en) * 2020-04-26 2020-08-04 上海市农业科学院 Efficient hybrid japonica rice breeding method capable of reducing seed production cost

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Application publication date: 20130123