CN102861113A - Anti-inflammatory activity part of boat-fruited scaphium seed and preparation method of anti-inflammatory activity part - Google Patents
Anti-inflammatory activity part of boat-fruited scaphium seed and preparation method of anti-inflammatory activity part Download PDFInfo
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- CN102861113A CN102861113A CN2012103824818A CN201210382481A CN102861113A CN 102861113 A CN102861113 A CN 102861113A CN 2012103824818 A CN2012103824818 A CN 2012103824818A CN 201210382481 A CN201210382481 A CN 201210382481A CN 102861113 A CN102861113 A CN 102861113A
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- 230000003110 anti-inflammatory effect Effects 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241001412231 Scaphium <angiosperm> Species 0.000 title abstract 3
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 23
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000000284 extract Substances 0.000 claims abstract description 16
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 4
- 210000000582 semen Anatomy 0.000 claims description 33
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 30
- 238000000605 extraction Methods 0.000 claims description 28
- 238000002481 ethanol extraction Methods 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 239000002260 anti-inflammatory agent Substances 0.000 claims 1
- 229940124599 anti-inflammatory drug Drugs 0.000 claims 1
- COTNUBDHGSIOTA-UHFFFAOYSA-N meoh methanol Chemical compound OC.OC COTNUBDHGSIOTA-UHFFFAOYSA-N 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract description 12
- 241000699670 Mus sp. Species 0.000 abstract description 10
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 abstract description 10
- 206010014025 Ear swelling Diseases 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000003187 abdominal effect Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- -1 Dichlorodiphenyl Acetate Chemical compound 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001412228 Scaphium lychnophorum Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000934878 Sterculia Species 0.000 description 1
- 241001503664 Sterculia nobilis Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
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- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003810 ethyl acetate extraction Methods 0.000 description 1
- 229960003699 evans blue Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000009438 liyan Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
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- 230000008961 swelling Effects 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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Abstract
The invention relates to an anti-inflammatory activity part discovered in boat-fruited scaphium seed extract and a preparation method of the anti-inflammatory activity part. The preparation method includes: sequentially extracting boat-fruited scaphium seed extract with cyclohexane, ethyl acetate and n-butyl alcohol, and partially drying the extracted water solution to obtain the anti-inflammatory activity part. Anti-inflammatory activity test of mice ear swelling model caused by dimethylbenzene and celiac seepage model caused by acetic acid shows that the part has evident anti-inflammatory activity.
Description
Invention field
The present invention relates to a kind of anti-inflammatory activity position of from the ethanol extraction of Chinese medicine Semen Sterculiae Lychnophorae, finding and the method for preparing this active site.
Background technology
Semen Sterculiae Lychnophorae has another name called Da Dongguo, is the dry mature seed of Sterculiaceae Sterculia plant lamp seed sterculia nobilis Sterculia lychnophora Hance, and main product ground is positioned at the states such as Vietnam, Thailand, Malaysia and India.At present, southern china is introduced a fine variety, but most of Semen Sterculiae Lychnophorae medical material that China uses now leans on from above-mentioned national import.The traditional Chinese medical science thinks that Semen Sterculiae Lychnophorae has the effect of clearing heat and moistening lung, liyan jiedu, loosening bowel to relieve constipation.Its main pharmacological be antiinflammatory, antibiotic, antiviral etc. (Du Lijun, etc. Chinese crude drug, 1995,18 (8): 409-410; Qu Benqin, etc. Chinese Medical Journal, 1954, (5): 337-340).At present the chemical constituent in the Semen Sterculiae Lychnophorae of report mainly comprise saccharide, flavonoid, alkaloids and fatty acid (Chen Jianmin, etc. Chinese crude drug, 1995,18 (11): 567-569; Chen Jianmin, etc. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1996,21 (1): 39-41; Wang RF, et a1.Phytochemistry, 2003,63 (4): 475-478).Have been reported and claim the anti-inflammatory activity composition of Semen Sterculiae Lychnophorae to be mainly polysaccharide, but polysaccharide anti-inflammatory activity when oral and local topical not obvious (Du Lijun, etc. Chinese crude drug, 1995,18 (8): 409-410; Chen Jianmin, etc. Chinese crude drug, 1994,17 (8): 32-34).In view of traditional administering mode of Semen Sterculiae Lychnophorae is oral administration behind the warm water soaking, we think that the anti-inflammatory activity composition of Semen Sterculiae Lychnophorae can not be attributable simply to polysaccharide, and other compositions may be arranged in addition, should be the active sites that multicomponent consists of.For this reason, we are studied the anti-inflammatory activity position of Semen Sterculiae Lychnophorae, and have inquired into the preparation method of its active site, for further development and utilization Semen Sterculiae Lychnophorae lays the foundation.
Summary of the invention
Purpose of the present invention is for providing anti-inflammatory activity position of a kind of Semen Sterculiae Lychnophorae and preparation method thereof.It is characterized by: Semen Sterculiae Lychnophorae extract is extracted with cyclohexane extraction, ethyl acetate and n-butyl alcohol successively, be anti-inflammatory activity position (Fig. 1) after the aqueous solution part drying after the extraction.Mice ear model due to the employing dimethylbenzene and the Abdominal effusion model due to the acetic acid carry out anti-inflammatory activity research to this active site.Activity research is the result prove, under Isodose, this anti-inflammatory activity position obviously is better than blank and Semen Sterculiae Lychnophorae 95% ethanol extraction to the inhibitory action of inflammation.
Description of drawings
The preparation flow at Fig. 1 Semen Sterculiae Lychnophorae anti-inflammatory activity position
Referring to Fig. 1, with the Semen Sterculiae Lychnophorae pulverizing medicinal materials, cross 10 mesh sieves.The gained powder adds 95% soak with ethanol of 4 times of amounts after half an hour, heating and refluxing extraction 3 times, and extraction time is respectively 2h, 2h and 1h.Gained extracting solution concentrating under reduced pressure obtains 95% ethanol extraction.95% extract mixture is suspended from isopyknic (weight: in methanol volume), add isopyknic cyclohexane extraction again and extract 5 times.Then, the methanol layer reclaim under reduced pressure to residual without methanol, is added the distilled water suspendible of 2 times of volumes, use successively with the isopyknic ethyl acetate of aqueous solution and n-butyl alcohol to extract.With the extraction after aqueous solution partly carry out lyophilization after, the anti-inflammatory activity position that namely gets Semen Sterculiae Lychnophorae.
The specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment
1. the preparation at Semen Sterculiae Lychnophorae anti-inflammatory activity position
Semen Sterculiae Lychnophorae 2.7kg crosses 10 mesh sieves after pulverizing.Coarse powder extracts 3 times with 95% alcohol heating reflux of 4 times of amounts, and the time is respectively 2h, 2h and 1h.The extracting solution concentrating under reduced pressure gets alcohol-extracted extract 3240g.Get extractum 300g, with 300ml methanol suspendible, suspension adds the extraction of equal-volume cyclohexane extraction again.After the cyclohexane extraction extraction fully, that the methanol layer reclaim under reduced pressure is extremely residual without methanol.Use again 200ml distilled water suspendible, use successively ethyl acetate and n-butyl alcohol with aqueous solution equivalent to extract.Get cyclohexane extraction extraction part 98.0g, ethyl acetate extraction part 12.7g, n-butanol portion 28.8g and the rear water-soluble part 131g of extraction after reclaiming the each several part solvent.Water-soluble part is the active site of Semen Sterculiae Lychnophorae after this extraction.
2. the anti-inflammatory activity research at Semen Sterculiae Lychnophorae anti-inflammatory activity position
Mice ear model due to the employing dimethylbenzene and the Abdominal effusion model due to the acetic acid carry out anti-inflammatory activity relatively to Semen Sterculiae Lychnophorae 95% ethanol extraction and the rear water-soluble part of extraction.
(1) mice ear experiment
Get 33 of mices, be divided at random 3 groups (grouping and dosage see Table 1).Ig administration, dosage are 0.2mL/10g, administration every day 2 times, successive administration 5 times.Behind the last administration 1h, dimethylbenzene 40 μ l evenly are applied to two sides, left ear front and back.30min tailing edge auricle baseline is cut two ears, uses the card punch of 8mm diameter in the same position punching of two ears, claims circle auricle weight.Be calculated as follows Mus ear swelling degree and inhibitory rate of intumesce.
Swelling (W)=left auricle weight-auris dextra sheet weight
Inhibitory rate of intumesce (%)=(W contrast-W administration)/W contrast * 100%
Each is organized data and represents that with Mean ± SD analyze between group with the t check and carry out statistical procedures, statistical result sees Table 1.
Water-soluble part xylol causes the impact of mice ear after table 1. Semen Sterculiae Lychnophorae 95% ethanol extraction and the extraction
Annotate: 1 animal of water-soluble portion group is unexpected dead in the administration process after the extraction.Compare with the blank group,
*P＜0.01.
(2) mouse peritoneal oozes out experiment
Get 36 of mices, grouping and administration are tested with mice ear.Behind the last administration 1h, tail vein injection 1% azovan blue physiological salt liquid 0.1mL/10g, and lumbar injection 0.6% acetic acid 0.2mL immediately.Behind the 20min mice is taken off cervical vertebra and put to death, Intraperitoneal injection 8mL normal saline is gently rubbed 30sec, cuts off stomach wall, draws the abdominal cavity cleanout fluid in test tube, and the centrifugal 10min of 3000rpm, supernatant survey absorbance (OD) in spectrophotometer 590nm place.Calculate the Abdominal effusion suppression ratio with following formula:
Ooze out suppression ratio=(OD contrast-OD administration)/OD contrast * 100%
Result of calculation is learned processing by statistics, sees Table 2.
Water-soluble part Dichlorodiphenyl Acetate causes the impact that mouse peritoneal oozes out after table 2. Semen Sterculiae Lychnophorae 95% ethanol extraction and the extraction
Annotate: 2 animals of blank group and 1 animal of 95% ethanol extraction group are unexpected dead in the administration process.Compare with the blank group,
*P＜0.01.
As can be seen from the above results, water-soluble part xylol causes mice ear and Dichlorodiphenyl Acetate and causes suppression ratio that mouse peritoneal oozes out respectively up to 64.5% and 69.0% after the extraction, compare with the blank group and to have statistical significant difference, and apparently higher than 95% ethanol extraction, prove that the rear water-soluble part of extraction is the anti-inflammatory activity position of Semen Sterculiae Lychnophorae.
Claims (8)
1. a kind of anti-inflammatory activity position of Semen Sterculiae Lychnophorae and the application in field of medicaments thereof.
2. anti-inflammatory activity position according to claim 1 and application thereof, application wherein relates to pharmacy, food and field of health care products.
According to claim 1 with 2 described anti-inflammatory activity position and application thereof, application wherein refers to the application in producing anti-inflammatory drug and anti-inflammatory health product.
4. the preparation method at a Semen Sterculiae Lychnophorae anti-inflammatory activity position, may further comprise the steps: Semen Sterculiae Lychnophorae extract is used cyclohexane extraction successively, and ethyl acetate and n-butyl alcohol extract, and are the anti-inflammatory activity position after the aqueous solution part drying after the extraction.
5. preparation method according to claim 4, may further comprise the steps: with Semen Sterculiae Lychnophorae extract methanol suspendible, after suspension extracts with cyclohexane extraction, be recycled to without methanol methanol layer residual, add again the water suspendible, extract with ethyl acetate and n-butyl alcohol successively, be the anti-inflammatory activity position after the aqueous solution part drying after the extraction.
According to claim 4 with 5 described preparation methoies, wherein Semen Sterculiae Lychnophorae extract is ethanol extraction.
According to claim 4 with 5 described preparation methoies, wherein Semen Sterculiae Lychnophorae extract is 95% ethanol extraction.
8. preparation method according to claim 4, may further comprise the steps: with the Semen Sterculiae Lychnophorae medicinal powder with 95% soak with ethanol of 4 times of amounts after half an hour, heating and refluxing extraction 3 times, extraction time is respectively 2h, 2h and 1h, gained extracting solution concentrating under reduced pressure obtains 95% ethanol extraction, 95% extract mixture is suspended from isopyknic methanol, adding isopyknic cyclohexane extraction extracts 5 times again, then, the methanol layer reclaim under reduced pressure to residual without methanol, is added the distilled water suspendible of 2 times of volumes, use successively with the isopyknic ethyl acetate of aqueous solution and n-butyl alcohol to extract, with the extraction after aqueous solution partly carry out lyophilization after, the anti-inflammatory activity position that namely gets Semen Sterculiae Lychnophorae.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114716406A (en) * | 2022-04-15 | 2022-07-08 | 山东省农业科学院 | Semen Scaphii Lychnophori extract with xanthine oxidase inhibiting activity, and its preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381418B (en) * | 2007-09-05 | 2012-07-04 | 光明乳业股份有限公司 | Sterculia seed single polysaccharide SPA, preparation method and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381418B (en) * | 2007-09-05 | 2012-07-04 | 光明乳业股份有限公司 | Sterculia seed single polysaccharide SPA, preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 李丽娟: "《药物合成技术》", 31 July 2010 * |
| 李娜等: "胖大海药学研究概况", 《安徽农业科学》 * |
| 杜力军等: "国产与进口胖大海对小鼠抗炎和小肠推进作用比较", 《中药材》 * |
| 陈建民等: "胖大海中多糖的成分分析和含量测定", 《中药材》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114716406A (en) * | 2022-04-15 | 2022-07-08 | 山东省农业科学院 | Semen Scaphii Lychnophori extract with xanthine oxidase inhibiting activity, and its preparation method and application |
| CN114716406B (en) * | 2022-04-15 | 2023-04-25 | 山东省农业科学院 | Semen Scaphii Lychnophori extract with xanthine oxidase activity inhibiting effect, and its preparation method and application |
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