CN108704036A - A kind of Chinese traditional compound medicine and preparation method thereof for treating gout - Google Patents
A kind of Chinese traditional compound medicine and preparation method thereof for treating gout Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a kind of Chinese traditional compound medicine and preparation method thereof for treating gout, which is made of following bulk pharmaceutical chemicals：The Herba polygoni hydropiperis extract obtained after the Chinese photinia leaf extract, polygonum flaccidum or the corresponding parts by weight that are obtained after the Herba Verbenae, folium photiniae or the corresponding parts by weight that are obtained after Verbena officinalis or corresponding parts by weight are extracted are extracted are extracted.The Chinese traditional compound medicine that the present invention studies is for treating gout with the advantages such as recurrence rate is low, few side effects, prolonged application have no drug resistance；And the proportioning of herbal mixture each component and drug dose are subjected to science adjustment and innovation, so that each herbal medicine is played its maximum drug effect；The Chinese traditional compound medicine is cheap simultaneously.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, specifically, the present invention relates to a kind of Chinese traditional compound medicine for treating gout,
The invention further relates to the preparation methods of the Chinese traditional compound medicine.
Background technology
Gout (Gout) is the systemic disease caused by a kind of purine metabolic disturbance, metabolism disorder or urine of the human body to purine
The excretion of acid is reduced, and uric acid is caused in blood to increase, urate crystal be deposited in the more rich connective tissue of mucopolysaccharide amount in matrix,
So as to cause gout in cartilage and articular cavity, its main feature is that hyperuricemia and characteristic acute arthritis, recurrent exerbation can be made
At tophus precipitation and gouty nephropathy.In recent years one of the high morbidity of China both urban and rural residents is had become, and with other complication,
Seriously affect people's health life.China's Patients with Hyperuricemia is up to 1.2 hundred million at present, about 17,000,000 people of patient with gout.Gout
Generation is related to gender and age, is more common in the middle-aged and the old, accounts for about 90%, the onset peak age is 40~50 years old, male to female ratio
About 20:1.Studies have shown that south General Population hyperuricemia is up to 26.8%, and the north is up to 25.3%.Patient with gout at present
Average age：48.28 years old, and it is then within 2015 48.76 years old, patient groups are in apparent rejuvenation trend；Male is 47.95
Year, women are 53.14 years old, and the average age of female patient is higher than male patient.
Currently, for acute and chronic patient with gout mainly by using reducing the chemical drugs of uric acid level as remedy measures,
Existing drug has stronger pharmacological action, but also shows various apparent side effects, harmful to human function and damage device
Official.Many patients can not reach preferable curative effect in clinical application, in addition, in developing country, some effective drugs
Using the factors such as limited and part price is higher, the extensive use of such product is limited.Therefore treatment is developed from Chinese medicine
Specific anti-gout drugs are imitated, will be of great significance.
Invention content
In view of this, the purpose of the present invention is to provide a kind of Chinese traditional compound medicines for treating gout.
To achieve the above object, this invention takes technical solutions below：A kind of Chinese traditional compound medicine for treating gout,
The Chinese traditional compound medicine is made of following bulk pharmaceutical chemicals：The Herba Verbenae that is obtained after Verbena officinalis or corresponding parts by weight are extracted,
It is obtained after the Chinese photinia leaf extract, polygonum flaccidum or the corresponding parts by weight that are obtained after folium photiniae or corresponding parts by weight are extracted are extracted peppery
Knotweed extract.
Specifically, which is made of the bulk pharmaceutical chemicals of following parts by weight：2-10 parts of Verbena officinalis, folium photiniae 2-10
Part, 6-30 parts of polygonum flaccidum.
Preferably, which is made of the bulk pharmaceutical chemicals of following parts by weight：10 parts of Verbena officinalis, 10 parts of folium photiniae,
30 parts of polygonum flaccidum.
Wherein, which is oral preparation.
Preferably, the oral preparation is capsule, granule, oral solution or powder.
Further, the capsule is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight at 75 DEG C
Under the conditions of, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure to obtain the final product
Herba Verbenae；Take folium photiniae dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 80 DEG C, refluxing extraction 2 times, 1 is small every time
When, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material weight
10 times of 75% ethyl alcohol is under the conditions of 60 DEG C, refluxing extraction 2 times, 1.5 hours every time, merges extracting solution, filtering, filtrate decompression recycling
Ethyl alcohol is dried under reduced pressure up to Herba polygoni hydropiperis extract, mix and crush with Herba Verbenae and Chinese photinia leaf extract, be added starch with
Magnesium stearate, mixing are canned to obtain capsule.
Further, the granule is made by the following method：Verbena officinalis dosing material 5 times of 75% alcohol reflux of weight is taken to carry
It takes 3 times, 1 hour every time, extracting solution merged, and filtering, decompression filtrate recycling ethanol, crushed after being dried is to get Herba Verbenae；
Folium photiniae is taken, is added water to cook 3 times, 2 hours every time, is merged decocting liquid, filter, be concentrated under reduced pressure, add ethanol precipitation, stand, supernatant
Ethyl alcohol is recovered under reduced pressure in liquid, and crushed after being dried is to get Chinese photinia leaf extract；Polygonum flaccidum dosing material 5 times of 75% alcohol reflux of weight is taken to carry
Take 3 times, 1 hour every time, extracting solution merged, filtering, decompression filtrate recycling ethanol, crushed after being dried, with Herba Verbenae and
Chinese photinia leaf extract is uniformly mixed, and low-substituted hydroxypropyl cellulose 3% is added, water is added to moisten, is made particle, it is dry to get
Granula.
Further, the oral solution is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight at 75 DEG C
Under the conditions of, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure to obtain the final product
Herba Verbenae；Take folium photiniae dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 80 DEG C, refluxing extraction 2 times, 1 is small every time
When, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material weight
10 times of 75% ethyl alcohol is under the conditions of 60 DEG C, refluxing extraction 2 times, 1.5 hours every time, merges extracting solution, filtering, filtrate decompression recycling
Ethyl alcohol is dried under reduced pressure up to Herba polygoni hydropiperis extract, is mixed with Herba Verbenae and Chinese photinia leaf extract, is concentrated and dried, is dissolved in water
Manufactured oral solution.
Further, the powder is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight in 75 DEG C of items
Under part, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure up to horse
Whip grass extract；Take folium photiniae dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time,
Merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material weight 10
75% ethyl alcohol is under the conditions of 60 DEG C again, refluxing extraction 2 times, 1.5 hours every time, merges extracting solution, filtering, and filtrate decompression recycles second
Alcohol is dried under reduced pressure up to Herba polygoni hydropiperis extract, mixes and crush with Herba Verbenae and Chinese photinia leaf extract, crosses 120 mesh sieve, obtains
Powder.
The pharmacological property of each bulk pharmaceutical chemicals is as follows in the present invention：
Verbena officinalis：Herba Verbenae, no obvious toxic-side effects, therapeutic domain be concentrated mainly on malaria, hepatitis, dysentery,
Mouth infection and gynecological disease etc..
Folium photiniae：Folium photiniae has apparent stable and cooling effect, and has analgesia, anti-inflammatory and antitumaous effect；It is blue to leather
Family name's positive bacteria, negative bacterium and saccharomycete have inhibiting effect；In addition, also having decompression antirheumatic, antitumor and other effects.
Polygonum flaccidum：Acrid flavour, temperature；With expelling wind and removing dampness, scattered silt analgesic, removing toxicity for detumescence and other effects；Be mainly used for immediately, stomach
Inflammation, diarrhea, tinea pedis, Jie Xian, rheumatic arthritis, functional uterine bleeding；The symptoms such as venomous snake bite, skin eczema are controlled in external application；This
Outside, also there are the multiple biological activities such as antimicrobial, desinsection, anti-oxidant, antitumor.
Dosage form
The dosage form of Chinese traditional compound medicine of the present invention includes capsule, granule, powder, oral solution etc..In addition, the present invention is multiple
Square Chinese medicine preparation may also include pharmaceutical carrier.
Should also include allowing to make on galenic pharmacy in Chinese traditional compound medicine of the present invention other than including effective drug ingedient
The effect of carrier such as auxiliary material, these carriers is contributed to the preparation that is processed into of active ingredient, and delivers active ingredient
To internal site of action.
Dosage
The dosage of administration should be according to the age of patient, weight, health status, the course for the treatment of, individual difference and dosage form and administration way
Depending on the difference of diameter.Those skilled in the art can determine the optimum range of each component effective dose according to individual need.
Compared with prior art, the present invention has the advantages that：
The herbal mixture prescription that the present invention studies is for treating gout；Herbal mixture prescribed treatment gout has recurrence rate
Low, few side effects, prolonged application such as have no drug resistance at the advantages；And by the proportioning of herbal mixture each component and drug dose carry out section
Adjustment and innovation is learned, each herbal medicine is made to play its maximum drug effect；Compound gout soup and its Chinese traditional compound medicine system are found for the first time
Agent has good curative effect in the treatment of anti-trioxypurine, and causes mouse high lithemia model, Dichlorodiphenyl Acetate cause small by using Monosodium urate
Mouse writhing model, acetic acid cause mouse peritoneal capillary permeability to increase and have carried out antigout experimental verification in model.Experiment knot
Fruit shows the use of compound gout soup, to reducing mouse uric acid content, analgesia and anti-inflammatory aspect there is significant treatment to imitate respectively
Fruit；The Chinese traditional compound medicine is cheap.
Specific implementation mode
The following detailed description of the present invention specific implementation mode, as part of this specification, by embodiment come
Illustrate that the principle of the present invention, other aspects of the present invention, feature and its advantage will become apparent by the detailed description.
First part：Chinese traditional compound medicine, preparation and its application method of the present invention
Each Chinese traditional compound medicine prescription and dosage form are as shown in table 1 in embodiment 1-8：
Chinese traditional compound medicine prescription and dosage form in 1 embodiment 1-8 of table
1 preparation method of embodiment：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2
It is secondary, 1.5 hours every time, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Take stone
Nanmu leaf dosing material 10 times of 75% ethyl alcohol of weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filters,
Decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight at 60 DEG C
Under the conditions of, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure to obtain the final product
Herba polygoni hydropiperis extract is mixed and is crushed with Herba Verbenae and Chinese photinia leaf extract, and starch and magnesium stearate, mixing, tank is added
Fill to obtain capsule.Every capsule medicated amount 0.4g, 4 tablets each time, 3 times a day.
2 preparation method of embodiment：It takes 5 times of 75% alcohol reflux of Verbena officinalis dosing material weight to extract 3 times, 1 hour every time, carries
It takes liquid to merge, filters, decompression filtrate recycling ethanol, crushed after being dried is to get Herba Verbenae；Folium photiniae is taken, adds water to cook 3
It is secondary, 2 hours every time, merge decocting liquid, filter, be concentrated under reduced pressure, add ethanol precipitation, stand, ethyl alcohol is recovered under reduced pressure in supernatant, dry
After crush to get Chinese photinia leaf extract；It takes 5 times of 75% alcohol reflux of polygonum flaccidum dosing material weight to extract 3 times, 1 hour every time, extracts
Liquid merges, and filtering, decompression filtrate recycling ethanol, crushed after being dried is uniformly mixed with Herba Verbenae and Chinese photinia leaf extract,
Low-substituted hydroxypropyl cellulose 3% is added, water is added to moisten, particle is made, it is dry to get granule.8g, 3 times a day.
Embodiment 3-5 preparation methods：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2
It is secondary, 1.5 hours every time, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Take stone
Nanmu leaf dosing material 10 times of 75% ethyl alcohol of weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filters,
Decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight at 60 DEG C
Under the conditions of, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure to obtain the final product
Herba polygoni hydropiperis extract is mixed with Herba Verbenae and Chinese photinia leaf extract, is concentrated and dried, is dissolved in oral solution made of water.Daily 3
It is secondary, each 20g.
Embodiment 6-8 preparation methods：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2
It is secondary, 1.5 hours every time, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Take stone
Nanmu leaf dosing material 10 times of 75% ethyl alcohol of weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filters,
Decompression filtrate recycling ethanol is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight at 60 DEG C
Under the conditions of, refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure to obtain the final product
Herba polygoni hydropiperis extract is mixed and is crushed with Herba Verbenae and Chinese photinia leaf extract, is crossed 120 mesh sieve, is obtained powder.It is oral, once
3-6g, twice a day.
Second part：The pharmaceutical research of 1 Chinese traditional compound medicine of the present invention of test example
1 experimental design
1.1 empirical factors and experimental method
2 factor level table of table (dosage/g)
3 U of table6(64) experimental program and observation index (g)
| Experiment number | Verbena officinalis | Folium photiniae | Polygonum flaccidum |
| No. 1 medicine group | 0 | 2 | 12 |
| No. 2 medicine groups | 2 | 6 | 30 |
| No. 3 medicine groups | 4 | 10 | 6 |
| No. 4 medicine groups | 6 | 0 | 24 |
| No. 5 medicine groups | 8 | 4 | 0 |
| No. 6 medicine groups | 10 | 8 | 18 |
The influence of 1.2 pairs of mouse serum uric acid levels
The foundation of mouse high lithemia model
It takes 2g uric acid to be added in 400ml boiling water, then adds 1moll-1Sodium hydroxide solution makes pH be 7.4, is heated to 95 DEG C,
Then, at room temperature after cooling and stirring, 6h is placed at 4 DEG C, and sodium urate crystals are filtered by vacuum to obtain after precipitation is precipitated.It finally will urine
Sour sodium crystallization is put into 80 DEG C of baking ovens dry.1g sodium urate crystals are weighed before use, and physiological saline 20ml is added after being fully ground
It is made into 50mgml-1's.After the last administration, in addition to blank control group, Monosodium urate suspension is injected intraperitoneally in remaining each group, complete
At modeling.
Kunming mouse (half male and half female) 90, is randomly divided into blank control group after weighing and negative control group, the positive are right
According to group (allopurinol group), 1-6 Chinese medical extracts group (each bulk pharmaceutical chemicals extraction is as described in embodiment 3-5)；In addition to blank group,
Remaining each group is with physiological saline 30g/ (kgd-1) gavage 1 time/d, continuous 7d；Positive controls give allopurinol (40mgkg-1) gavage, each extract group of Chinese medicine gives since 5d, Chinese medicine gavage, dosage 0.75g/ (kgd-1), continuous 7d, last
60min mouse pluck eyeball and take blood after administration, extract eyeball and blood, blood sample 3000rpm is taken to centrifuge 15 minutes, take supernatant and according to urine
The teachings of sour (UA) detection kit (phosphotungstic acid colorimetric method), measure and record uric acid level, and t is examined between carrying out group.
1.3 pairs of glacial acetic acid cause the influence of mouse writhing reaction
Kunming mouse 90 (half male and half females) is randomly divided into model group, positive controls (7.5 × 10-2mg·kg-1),
6 dosage groups of herbal mixture (each bulk pharmaceutical chemicals extraction is as described in embodiment 3-5), totally 9 groups, every group 10, herbal mixture ethyl alcohol carries
Take object 6 dosage groups difference gavages, each gavage 0.2mL, positive controls acetaminophen solution gavage, every time
0.2mL negative control groups and blank control group then use physiological saline gavage, continuous gavage 5d.Last 30min after gavage respectively
Establish pain model respectively in addition to blank control group afterwards, every mouse peritoneal injects 0.6% glacial acetic acid solutions of 0.1mL, and record is made
The writhing number of mouse and each group mouse writhing inhibiting rate in 20min after mould, and measure serum NO content.
1.4 Dichlorodiphenyl Acetates cause the influence that mouse peritoneal capillary permeability increases
Kunming mouse 90 (half male and half females) is randomly divided into model group, positive controls (405mgkg-1), in compound
6 dosage groups of medicine (each bulk pharmaceutical chemicals extraction is as described in embodiment 3-5), totally 9 groups, every group 10, the corresponding liquid of each group gavage,
20ml·kg-1, the isometric physiological saline of model group gavage is administered once daily, animal fasting before successive administration 5d last doses
16h, after 1h is administered, every 0.5% Evans blue 0.1ml10g of mouse tail vein injection-10.2ml is injected intraperitoneally in weight immediately
0.6% glacial acetic acid solution, mouse is put to death immediately after 20min, with 2 cleaning abdominal cavities of 6ml physiology salts moisture, collects washing lotion, Yu Zi
The OD values of washing lotion are measured at outer spectrophotometer 590nm.
2. result
2.1 mouse high lithemia model experiment results：It is shown in Table 4.
4 herbal mixture each component extract of table to mouse uric acid level influence (N=10)
2.2 glacial acetic acid cause mouse writhing reaction model experimental result：It is shown in Table 5.
Table 5 to glacial acetic acid cause mouse writhing effect (N=10)
2.3 acetic acid cause mouse peritoneal capillary permeability to increase experimental result：It is shown in Table 6.
Influence that table 6 increases mouse peritoneal capillary permeability (N=10)
Note：For administration group compared with negative control group, * indicates that significant difference (P < 0.05), * * indicate difference highly significant (P
< 0.01).
For negative control group compared with blank control group, # indicates that significant difference (P < 0.05), ## indicate difference highly significant
(P < 0.01).
The results show that herbal mixture prescription is reducing uric acid content, anti-inflammatory, ease pain with conspicuousness drug effect.
By in each experimental data, the dosage of Verbena officinalis, folium photiniae and polygonum flaccidum is respectively as independent variable X1、X2、X3；NO contents,
Absorbance OD values and mouse uric acid content are respectively dependent variable Y1、Y2、Y3, multiple stepwise regression is carried out with 25 statistical softwares of SPSS
Analyzing processing obtains and tests correspondence between each evaluation index and influence factor.The recurrence side of evaluation index and formulation factors
Cheng Wei：
Y1=151-3.18*X1-0.857*X2-0.679*X3(r=0.9976, P=0.01294)
Y2=0.105-0.003*X1-0.0012*X2-0.0005*X3(r=0.9943, P=0.01181)
Y3=604-5.68*X1-15.8*X2-1.92*X3(r=0.9315, P=0.01348)
And according to regression equation it is found that in the pharmacodynamic experiment of anti-trioxypurine party, Chinese medicine is to the percentage contribution of prescription：Stone
Nanmu leaf > polygonum flaccidum > Verbena officinalis.
Above-mentioned regression equation optimizes the extreme value of solution, and the actual conditions of comprehensive Chinese traditional compound medicine determine optimization
Proportioning of the compound antigout Chinese medicine each component in prescription be：Verbena officinalis: folium photiniae: polygonum flaccidum=10g: 10g: 30g.
In addition, experimental animal during administration can normal diet, excretion, weight has certain increase, and without obviously not
Good reaction, it can thus be appreciated that the herbal mixture prescription is without obvious toxic-side effects.
3 experiment conclusions
It can be seen that relative to model group, the hyperuricemia of herbal mixture dosage group obtains significant improvement, above-mentioned
Experiments have shown that Chinese traditional compound medicine has notable curative effect, and drug safety in treating gout.
Claims (9)
1. a kind of Chinese traditional compound medicine for treating gout, which is characterized in that the Chinese traditional compound medicine is made of following bulk pharmaceutical chemicals：Horse
The stone obtained after the Herba Verbenae, folium photiniae or the corresponding parts by weight that are obtained after whip grass or corresponding parts by weight are extracted are extracted
Nanmu leaf extract, polygonum flaccidum or corresponding parts by weight it is extracted after obtained Herba polygoni hydropiperis extract.
2. it is according to claim 1 treatment gout Chinese traditional compound medicine, which is characterized in that the Chinese traditional compound medicine by with
The bulk pharmaceutical chemicals of lower parts by weight form：2-10 parts of Verbena officinalis, 2-10 parts of folium photiniae, 6-30 parts of polygonum flaccidum.
3. it is according to claim 2 treatment gout Chinese traditional compound medicine, which is characterized in that the Chinese traditional compound medicine by with
The bulk pharmaceutical chemicals of lower parts by weight form：10 parts of Verbena officinalis, 10 parts of folium photiniae, 30 parts of polygonum flaccidum.
4. according to the Chinese traditional compound medicine of any treatment gout in claim 1-3, which is characterized in that it is oral system
Agent.
5. the Chinese traditional compound medicine for the treatment of gout according to claim 4, which is characterized in that the oral preparation is capsule
Agent, granule, oral solution or powder.
6. the preparation method of the Chinese traditional compound medicine for the treatment of gout according to claim 5, which is characterized in that the capsule
Agent is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2 times, every time
1.5 hours, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Folium photiniae is taken to add
10 times of 75% ethyl alcohol of medicinal material weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filtering, and filtrate subtracts
Receipts ethyl alcohol is pushed back, is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 60 DEG C,
Refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure and is extracted up to polygonum flaccidum
Object is mixed and is crushed with Herba Verbenae and Chinese photinia leaf extract, and starch and magnesium stearate is added, and mixing is canned to obtain capsule
Agent.
7. the preparation method of the Chinese traditional compound medicine for the treatment of gout according to claim 5, which is characterized in that the particle
Agent is made by the following method：5 times of 75% alcohol reflux of Verbena officinalis dosing material weight is taken to extract 3 times, 1 hour every time, extracting solution closed
And filter, decompression filtrate recycling ethanol, crushed after being dried is to get Herba Verbenae；Folium photiniae is taken, adds water to cook 3 times, often
Secondary 2 hours, merge decocting liquid, filter, be concentrated under reduced pressure, add ethanol precipitation, stand, ethyl alcohol, powder after drying is recovered under reduced pressure in supernatant
It is broken to get Chinese photinia leaf extract；5 times of 75% alcohol reflux of polygonum flaccidum dosing material weight is taken to extract 3 times, 1 hour every time, extracting solution closed
And filter, decompression filtrate recycling ethanol, crushed after being dried is uniformly mixed with Herba Verbenae and Chinese photinia leaf extract, is added
Low-substituted hydroxypropyl cellulose 3%, adds water to moisten, and particle is made, dry to get granule.
8. the preparation method of the Chinese traditional compound medicine for the treatment of gout according to claim 5, which is characterized in that described oral
Liquid is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2 times, every time
1.5 hours, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Folium photiniae is taken to add
10 times of 75% ethyl alcohol of medicinal material weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filtering, and filtrate subtracts
Receipts ethyl alcohol is pushed back, is dried under reduced pressure up to Chinese photinia leaf extract；Take polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 60 DEG C,
Refluxing extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure and is extracted up to polygonum flaccidum
Object is mixed with Herba Verbenae and Chinese photinia leaf extract, is concentrated and dried, is dissolved in oral solution made of water.
9. the preparation method of the Chinese traditional compound medicine for the treatment of gout according to claim 5, which is characterized in that the powder
It is made by the following method：Take Verbena officinalis dosing material 8 times of 75% ethyl alcohol of weight under the conditions of 75 DEG C, refluxing extraction 2 times, every time 1.5
Hour, merge extracting solution, filtering, decompression filtrate recycling ethanol is dried under reduced pressure up to Herba Verbenae；Take folium photiniae dosing material
10 times of 75% ethyl alcohol of weight is under the conditions of 80 DEG C, refluxing extraction 2 times, 1 hour every time, merges extracting solution, filtering, and filtrate decompression is returned
Ethyl alcohol is received, is dried under reduced pressure up to Chinese photinia leaf extract；It takes polygonum flaccidum dosing material 10 times of 75% ethyl alcohol of weight under the conditions of 60 DEG C, flows back
Extraction 2 times 1.5 hours every time, merges extracting solution, filtering, and decompression filtrate recycling ethanol is dried under reduced pressure up to Herba polygoni hydropiperis extract,
It mixes and crushes with Herba Verbenae and Chinese photinia leaf extract, cross 120 mesh sieve, obtain powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110201073A (en) * | 2019-05-10 | 2019-09-06 | 新余学院 | A kind of Chinese medicine composition and its preparation method and application for treating urarthritis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110201073A (en) * | 2019-05-10 | 2019-09-06 | 新余学院 | A kind of Chinese medicine composition and its preparation method and application for treating urarthritis |
| CN110201073B (en) * | 2019-05-10 | 2021-09-14 | 新余学院 | Traditional Chinese medicine composition for treating gouty arthritis and preparation method and application thereof |
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Application publication date: 20181026 |