CN110201073A - A kind of Chinese medicine composition and its preparation method and application for treating urarthritis - Google Patents

A kind of Chinese medicine composition and its preparation method and application for treating urarthritis Download PDF

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CN110201073A
CN110201073A CN201910389536.XA CN201910389536A CN110201073A CN 110201073 A CN110201073 A CN 110201073A CN 201910389536 A CN201910389536 A CN 201910389536A CN 110201073 A CN110201073 A CN 110201073A
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chinese medicine
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urarthritis
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CN110201073B (en
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费洪新
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Xinyu University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Abstract

The present invention discloses a kind of Chinese medicine composition and its preparation method and application for treating urarthritis, which is made of each bulk pharmaceutical chemicals of following parts by weight: folium photiniae 5-8 parts by weight, Pine Nodular Branch 10-15 parts by weight, strophanthus divaricatus 20-25 parts by weight, water banyan 20-25 parts by weight, lycopodium calvatum 6-9 parts by weight, root of pungent litse fruit 5-8 parts by weight, Fructus Aesculi 5-7 parts by weight, rhizoma homalonemae 5-8 parts by weight.The Chinese medicine composition has effects that expelling wind and clearing away heat, damp dispelling and pain relieving, regulating qi and dredging collateral.Results of animal shows that the Chinese medicine composition can improve the arthroncus degree in urarthritis mouse toes portion, arthritis index, can also improve the immune organ organ index and oxidation resistance of urarthritis mouse, and without side-effects.It is proposed of the invention provides new technological means for treatment urarthritis.

Description

A kind of Chinese medicine composition for treating urarthritis and preparation method thereof and Using
Technical field
The present invention relates to a kind of Chinese medicine compositions and its preparation method and application, in particular to a kind of for treating gouty Arthritic Chinese medicine composition and its preparation method and application.The invention belongs to technical field of traditional Chinese medicines.
Background technique
Urarthritis (Gouty arthritis) be a kind of one of clinically common disease (Higashino T, Matsuo H, Okada Y, et al.A common variant of MAF/c-MAF, transcriptional factor Gene in the kidney, is associated with gout susceptibility [J] .Hum Cell, 2018,31 (1): 10-13.), mainly due to causing purine generation under the effect of the combined factors such as inherent cause, environmental factor, mental element It thanks to exception, lithate is caused to be deposited on the positions such as the joint capsule, synovial bursa, cartilage in body ankle joint and its foot joint, occur The reaction of damaged part aseptic inflammation, including damaged part is red, swollen, hot, pain, once ankle joint and its joint of foot joint Capsule, synovial bursa, cartilage are impaired serious, it will damaged part deformity occur, therefore, early diagnosis and therapy urarthritis is especially Important (Mirmiran R, Bush T, Cerra MM, et al.Joint Clinical Consensus Statement of the American College of Foot and Ankle Surgeons and the American Association Of Nurse Practitioners:Etiology Diagnosis and Treatment Consensus for Gouty Arthritis of the Foot and Ankle [J] .J Foot Ankle Surg, 2018,57 (6): 1207-1217.).
In recent years, with the continuous change of people's dietary structure, many people's feed lipid foods are relatively more, lead to gout Property arthritic disease incidence, illness rate increase year by year, it will constantly influence body health.Data shows that body lipid food is taken the photograph Enter excessive or body fat acid content to increase, body fat acid activation system can be activated and generate a large amount of free radicals, caused huge Phagocyte activates and leads to nuclear transcription factor-kappa B (NF- κ B) signal pathway activated, promotes Interleukin -1β (IL-1 β), tumor necrosis factor Son-α (TNF-α) release, IL-1 β, TNF-α can cause neutrophil accumulation, body promoted aseptic inflammation reaction occur, right Body tissue causes to damage (Hall CJ, Sanderson LE, Lawrence LM, et al.Blocking to some extent fatty acid-fueled mROS production within macrophages alleviates acute gouty Inflammation [J] .J Clin Invest, 2018,128 (5): 1752-1771.), at the same lipid food intake it is excessive or The increase of person's body fat acid content can also cause the reduction of lesion oxidation resistance, such as lesion that total antioxygen can occur Change ability (Total antioxidant capacity, T-AOC), superoxide dismutase (Superoxide dismutase, SOD), glutathione peroxidase (Glutathione peroxidase, GSH-PX) reduction and its oxidation product malonaldehyde (Malondialdehyde, MDA) increases.
Currently, the unclear urarthritis pathogenesis of the mankind.Data shows that urarthritis is mostly due to body There is exception in purine metabolism, causes uric acid to generate in vivo excessive or causes uric acid excretion to decline because renal function is low, Urate crystal is caused to be deposited on the bad place of the body blood circulatory system, such as joint and its periarticular tissue site blood It supplies the poor urate crystal that is conducive to deposit, urate crystal deposition can stimulate joint and its periarticular tissue aseptic occur Inflammatory reaction finally influences whether joint basic function.Studies have shown that can be by inhibiting aseptic scorching using nourshing kidney ginseng stilbene soup Disease reaction, enhancing oxidation resistance, Lai Gaishan body urarthritis related clinical manifestations (Han J, Xie Y, Sui F, et al.Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects [J] .Mol Med Rep, 2016,14 (3): 2589-2597.), it can be by inhibiting NF- κ B using aurantiamarin methyl chalcone The aseptic inflammation that signal pathway activated improves urarthritis mouse reacts (Ruiz-Miyazawa KW, Pinho-Ribeiro FA, Borghi SM, et al.Hesperidin Methylchalcone Suppresses Experimental Gout Arthritis in Mice by Inhibiting NF- κ B Activation [J] .J Agric Food Chem, 2018,66 (25): 6269-6280.)
Data shows that urarthritis is mostly excessive closely related with the release of body inflammatory medium, wherein IL-1 β, TNF- α, interleukin-6 (IL-6), IL-12 (IL-12) are the closely related inflammatory mediators of urarthritis.Studies have shown that containing There is nucleotide combination oligomerization domain (NACHT), rich in leucine repetitive structure domain (LRR) and hot protein structure domain (PYD) (NLRP3) the inflammatory body of albumen 3 inflammatory mediator of urarthritis can be mediated to generate, NLRP3 pass through NACHT polymerization Occurred conformation is reset and there is the identical PYD albumen of N-terminal to assemble and activate biological effect for the exposure of priming effect structural domain, induction, For NLRP3 through PYD in conjunction with the PYD of apoptosis associated speck-like protein (ASC), ASC passes through aspartic acid proteolytic enzyme -1 (Caspase-1) it raises structural domain (CARD) to combine pro-Caspase-1 and start to raise pro-Caspase-1, formation contains The NLRP3 inflammatory body of NLRP3, ASC and pro-Caspase-1 activate downstream Caspase-1, activate Caspase-1 (Caspase- Pro-IL-1 β can 1p10) be cut into cell factor IL-1 β, by macrophages secrete to extracellularly, IL-1 β's the latter will live Changing downstream NF- κ B can be led to so that other inflammatory mediators be mediated largely to generate using Taiwan green propolis (TGP) ethanol extract It crosses inhibition NLRP3 inflammatory body and improves urarthritis mouse related symptoms (Hsieh CY, Li LH, Rao YK, et al.Mechanistic insight into the attenuation of gouty inflammation by Taiwanese green propolis via inhibition of the NLRP3inflammasome[J].J Cell Physiol, 2019,234 (4): 4081-4094.).
It is generally believed that urarthritis is since the correlation that urate crystal is deposited on joint part and generates joint is faced Bed performance, can cause body the arthroncus degree in toes portion, arthritis exponential anomaly occur, body can also be caused to exempt from Epidemic disease organ organ index is abnormal and toes portion oxidation resistance reduces, and can more cause body toes portion IL-12, IL-1 β (Marchetti C, Swartzwelter B, Koenders MI, et al NLRP3 inflammasome inhibitor OLT1177 suppresses joint inflammation in murine models of acute arthritis[J] .Arthritis Res Ther, 2018,20 (1): 169.), IL-6, TNF-α albumen and gene level it is abnormal.Studies have shown that making With urarthritis model (Liu Y, Tang H, Liu X, et can be replicated in urate crystal injection of joint Al.Frontline Science:Reprogramming COX-2,5-LOX, and CYP4A-mediated arachidonic acid metabolism in macrophages by salidroside alleviates gouty arthritis[J].J Leukoc Biol, 2019,105 (1): 11-24.), promote animal the related clinical manifestations of urarthritis occur, including Red, swollen, hot, pain, dysfunction etc..Therefore, it is possible to use urate crystal injection of joint prepares urarthritis model, and Based on this model, observation application factor is dirty to urarthritis arthroncus degree, arthritis index, immune organ Device index, oxidation resistance, inflammatory mediator expression etc..
Theory of traditional Chinese medical science thinks that urarthritis belongs to the hot numbness of Chinese medicine, actively emphasizes ailment said due to cold or exposure, heat evil, damp evil, attacked by the cold and evil Collaterals of human joint causes collaterals of human joint qi and blood numbness to hinder and cause a disease.If eating and drinking without temperance, overfeeding delicious food is thermophilic with spleen deficiency Wine causes transporting and transforming function of the spleen and stomach too late, and essence of water and grain is not changed, damp evil, heat evil stasis blocking, blockage of main and collateral channels, arthralgia, so form gout Property arthritis.Therefore, the present invention is directed to the etiology and pathogenesis of urarthritis, and selection has expelling wind and clearing away heat, damp dispelling and pain relieving, qi-regulating The Chinese medicine (folium photiniae, Pine Nodular Branch, strophanthus divaricatus, water banyan, lycopodium calvatum, root of pungent litse fruit, Fructus Aesculi, rhizoma homalonemae) of dredging collateral and carry out section Prescription is learned, carries out the experimental study of prevention and treatment urarthritis, experiments have shown that Chinese medicine composition of the invention can be to gouty Arthritis has preferable therapeutic effect, and without side-effects.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese medicine compositions and preparation method thereof for treating urarthritis And application.
In order to achieve the above object, present invention employs following technological means:
The present invention discloses a kind of Chinese medicine composition and its preparation method and application that can be used for treating urarthritis, The Chinese medicine composition is total by folium photiniae, Pine Nodular Branch, strophanthus divaricatus, water banyan, lycopodium calvatum, root of pungent litse fruit, Fructus Aesculi, rhizoma homalonemae 8 taste medicinal materials composition, wherein folium photiniae, acrid flavour, hardship are mild-natured, return kidney channel, Liver Channel, and there is wind-dispelling strengthening tendons, dehumidifying bone strengthening, beneficial liver to stop The effect of itching；Pine Nodular Branch, acrid flavour, hardship are warm-natured, and return liver warp, kidney channel have effects that dispelling wind and removing obstruction in the meridians, damp dispelling and pain relieving；Strophanthus divaricatus, Sour, cool in nature, converge to the spleen channel, stomach meridian have removing pathogenic heat to promote digestion, effect stagnant except humidifying；Water banyan, bitter, micro-sweet, cool in nature, return lung Through, the spleen channel, stomach meridian, have effects that heat-clearing disappear it is stagnant, removing toxic substances promote the production of body fluid, driving away summer heat dehumidifying；Lycopodium calvatum, bitter, pungent, warm-natured, return liver warp, Have effects that eliminating dampness and dredging channels, relaxing muscles and tendons detumescence；Root of pungent litse fruit, acrid flavour, hardship are warm-natured, converge to the spleen channel, stomach meridian, and there is analgesic to help digestion, go The effect of gas eliminating dampness, warming middle-JIAO and strengthening the spleen, subdhing swelling and detoxicating；Fructus Aesculi, sweet in flavor, warm-natured, return liver warp, stomach meridian, in regulating qi-flowing for harmonizing stomach, width The effect of analgesic；Rhizoma homalonemae, mildly bitter flavor, pungent, return liver warp, kidney channel have effects that remove obstruction in channels to relieve pain, wind-dispelling detumescence, relaxing muscles and tendons dehumidifying. Comprehensive analysis, in the Chinese medicine composition of present invention treatment urarthritis, monarch drug in a prescription folium photiniae, Pine Nodular Branch mainly have wind-dispelling The effect of；Ministerial drug strophanthus divaricatus, water banyan mainly have effects that heat-clearing；Adjutant lycopodium calvatum, root of pungent litse fruit mainly there is dehumidifying to stop The effect of pain；Medicine Fructus Aesculi, rhizoma homalonemae is set mainly to have effects that regulating qi and dredging collateral.
The effect of for the clear Chinese medicine composition, the Chinese medicine composition is then carried out to urarthritis mouse mould The Primary Study of type, it is main investigate Chinese medicine composition to urarthritis mouse arthroncus degree, arthritis index, exempt from Epidemic disease organ organ index, oxidation resistance, IL-12, IL-1 β, IL-6, TNF-α albumen and gene expression, while also having investigated Drug composition evaluates controlling for traditional Chinese medicine composition for treating urarthritis to the organ index and mouse weight of important organ with this Therapeutic effect and side effect.
Studies have shown that Articular swelling and arthritis index it is particularly important for the evaluation of urarthritis (Li Ping, Song Juan, Li Qingyi wait Maize silk flavones extract in efficacy analysis [J] of improvement acute gouty arthritis in rats State's present age medicine, 2018,25 (34): 8-11；Goel N, Khanna V, Jain DK, et al.Gouty tophi Presenting as multinodular lateral inguinal swelling:A case report [J] .Diagn Cytopathol, 2018,46 (9): 801-803.).After this experiment, compared with negative control group, model group mouse joint Swelling and arthritis index are higher, illustrate that model group joint part inflammatory reaction is obvious, model foundation success, and model is steady It is fixed, subsequent experimental study can be carried out.After Chinese medicine composition high middle dosage treatment, the high middle dosage of Chinese medicine composition can be with The Articular swelling and arthritis index of urarthritis mouse are reduced, and Chinese medicine composition low dosage can not reduce pain The Articular swelling and arthritis index of wind arthritic mice illustrate that the high middle dosage of Chinese medicine composition can improve joint portion Position inflammatory reaction mitigates related symptoms caused by arthritis reacts.
It is generally believed that Immune Organs of Body includes central immune organ and peripheral immune organ.Central immune organ includes Thymus gland and marrow, peripheral immune organ include spleen, lymph node.This experiment evaluates Chinese medicine by measurement spleen index and thymus index The preliminary intervention effect that composition is immunoreacted urarthritis.The display of this experimental result, compared with negative control group, mould Type group mouse spleen index, thymus index are higher, illustrate that model group immune response is strong, in combination with model group urarthritis The Articular swelling and arthritis index of mouse are higher, and inflammatory reaction is obvious, and hints model group urarthritis inflammation is anti- Should be obvious, model stability strong with immune response.After the high middle dosage treatment of Chinese medicine composition, Chinese medicine composition senior middle school Dosage can reduce spleen index, thymus index, and can also reduce the Articular swelling and arthritis of urarthritis mouse Disease index illustrates that the high middle dosage of Chinese medicine composition can improve organism immune response and inflammatory reaction.
Data shows that urarthritis is mostly reduced with antioxidant ability of organism, this is more certainly with lesion generation By base have relationship (woods board for taking notes in ancient times, Wang Chenbin, Li Jian gout relieving capsule to acute gouty arthritis rat blood serum inflammatory factor and Influence [J] North China Polytechnics's journal (medicine) of oxidative stress, 2018,20 (5): 353-357.；Yang Kangdong, Lee send out Xin, Liang Yanchang waits pairing soup to influence [J] animal of PGE-2, MDA, SOD and MMP-3 in knee osteoarthritis rat articular liquid Advances in Medicine, 2018,39 (7): 63-66.；Dhanasekar C, Rasool M.Morin a dietary bioflavonol suppresses monosodium urate crystal-induced inflammation in an animal model of acute gouty arthritis with reference to NLRP3inflammasome hypo-xanthine Phospho-ribosyl transferase and inflammatory mediators [J] .Eur J Pharmacol, 2016,786:116-127.) it, removes free radical and needs a large amount of antioxidant reductase, therefore, contained by measuring antioxidant reductase Amount and its oxidation product content can be with the oxidation resistances of preliminary assessment urarthritis lesion.This experiment passes through detection Urarthritis toes portion SOD, GSH-PX, T-AOC, MDA content evaluates Chinese medicine composition to toes portion oxidation resistance Intervention effect.The experimental results showed that compared with negative control group, model group mouse toes portion SOD, GSH-PX, T-AOC content It is lower, increase with MDA, illustrates that the oxidation resistance in model group mouse toes portion weakens, the elimination efficiency decline of free radical, this It is related with the oxidation resistance reduction of toes portion.And after the treatment of Chinese medicine composition, the high middle dosage of Chinese medicine composition can increase Add urarthritis mouse toes portion SOD, GSH-PX, T-AOC content, reduce MDA content, gouty joint is improved with this The oxidation resistance in scorching mouse toes portion.
It is generally believed that aseptic inflammation, which occurs, in urarthritis reacts related with the lesion a large amount of inflammatory mediators of generation System, these inflammatory mediators be mostly IL-12, IL-6, IL-1 β, TNF-α etc. (Naredo E, Medina JP, Perez-Baos S, et al.Validation of Musculoskeletal Ultrasound in the Assessment of Experimental Gout Synovitis [J] .Ultrasound Med Biol, 2018,44 (7): 1516-1524.； Loustau C, Rosine N, Forien M, et al.Effectiveness and safety of anakinra in gout patients with stage 4-5chronic kidney disease or kidney transplantation: A multicentre, retrospective study [J] .Joint Bone Spine, 2018,85 (6): 755-760.), lead to Cross detection urarthritis toes portion IL-12, IL-6, IL-1 β, TNF-α content, can with preliminary assessment urarthritis without The inflammatory reaction of bacterium property is horizontal.The display of this experimental result, compared with negative control group, model group urarthritis mouse toes portion IL-12, IL-6, IL-1 β, TNF-α content obviously increase, and illustrate that model group inflammatory mediator content increases, aseptic inflammation reaction Obviously.After the high middle dosage treatment of Chinese medicine composition, the high middle dosage of Chinese medicine composition can reduce urarthritis mouse Toes portion IL-12, IL-6, IL-1 β, TNF-α content, and then reduce urarthritis mouse toes portion IL-12, IL-6, IL- 1 β, TNF-α are horizontal, weaken the reaction of urarthritis mouse toes portion's aseptic inflammation, in combination with Chinese medicine composition senior middle school Dosage can reduce the Articular swelling of urarthritis mouse, arthritis index, improve oxidation resistance, prompt Chinese medicine The high middle dosage of composition has urarthritis mouse toes portion Articular swelling, arthritis index, oxidation resistance Have an improvement result, this improvement result and the high middle dosage of Chinese medicine composition reduce urarthritis mouse toes portion IL-12, IL-6, IL-1 β, TNF-α content are closely related.
In order to evaluate the side effect of Chinese medicine composition, then this experiment detects each group mouse vitals organ index and body Change again.The experimental results showed that model group urarthritis mouse liver index, cardiac index, lung refer to compared with negative control group Number, cerebral index, renal index change unobvious (P > 0.05), and model group 0d, 7d, 14d, 21d mouse weight changes unobvious (P > 0.05), false injection group mouse liver index, cardiac index, Lung Exponent, cerebral index, renal index change unobvious (P > 0.05), vacation note It penetrates group 0d, 7d, 14d, 21d mouse weight and changes unobvious (P > 0.05).After traditional Chinese medicine composition for treating, with model group ratio Compared with positive controls, high dose group, middle dose group, low dose group mouse liver index, cardiac index, Lung Exponent, cerebral index, kidney refer to Number changes unobvious (P > 0.05), positive controls, high dose group, middle dose group, low dose group 0d, 7d, 14d, 21d mouse Weight changes unobvious (P > 0.05), illustrates that the Chinese medicine composition refers to urarthritis mouse liver index, cardiac index, lung Number, cerebral index, renal index and its weight do not influence, prompt Chinese medicine composition without side-effects, and indication can be in safe to use Drug composition treats urarthritis.
The above experiment shows that Chinese medicine composition height, middle dosage (23.48g/kg, 11.74g/kg) can improve gouty pass Arthroncus degree, the arthritis index for saving scorching mouse toes portion, can also improve the immune device of urarthritis mouse Official's organ index and oxidation resistance, can more improve the IL-12 in urarthritis mouse toes portion, IL-1 β, IL-6, TNF-α albumen and gene level, while the Chinese medicine composition is to the organ index of urarthritis mouse important organ and small Mouse weight illustrates that the traditional Chinese medicine composition for treating urarthritis is effective without influence, and the Chinese medicine composition is without side-effects.
On the basis of the studies above, the invention proposes a kind of for treating the Chinese medicine composition of urarthritis, The Chinese medicine composition is made of each bulk pharmaceutical chemicals of following parts by weight: folium photiniae 5-8 parts by weight, Pine Nodular Branch 10-15 parts by weight, Strophanthus divaricatus 20-25 parts by weight, water banyan 20-25 parts by weight, lycopodium calvatum 6-9 parts by weight, root of pungent litse fruit 5-8 parts by weight, Fructus Aesculi 5-7 parts by weight, rhizoma homalonemae 5-8 parts by weight.
Wherein, it is preferred that the Chinese medicine composition is made of each bulk pharmaceutical chemicals of following parts by weight: 6.5 weight of folium photiniae Part, 12 parts by weight of Pine Nodular Branch, 22.5 parts by weight of strophanthus divaricatus, 22.5 parts by weight of water banyan, 7.5 parts by weight of lycopodium calvatum, root of pungent litse fruit 6.5 parts by weight, 6 parts by weight of Fructus Aesculi, 6.8 parts by weight of rhizoma homalonemae.
Further, the invention also provides a kind of Chinese medicine systems for being used to treat urarthritis for being clinically suitable for Agent, auxiliary material needed for preparations shaping is added as Chinese medicine composition of the present invention, according to the routine side for preparing pharmaceutical preparation Method is made.
Wherein, it is preferred that the preparation is water decoction, pulvis, capsule, pill, granule, tablet or oral solution.
Further, the invention also provides a kind of methods for preparing the Chinese medicine composition, comprising the following steps:
(1) each bulk pharmaceutical chemicals are weighed according to parts by weight of the present invention, each bulk pharmaceutical chemicals are put into container after mixing, 10-20 times of drug weight of distilled water is added, after impregnating 1-2.5h, decocts 1-2.5h, it is cooling；
(2) by step (1) medical fluid filtered through gauze after cooling, first time filtrate and drug residue are obtained；
(3) the drug residue of step (2) is put into container, is repeated step (1) and (2) and is obtained second of filtrate, mixes the First-time filtrate and second of filtrate；
(4) dry, obtain the dry cream of the Chinese medicine composition.
Wherein, it is preferred that the filtered through gauze refers to successively uses 2 layers of filtered through gauze, 4 layers of filtered through gauze, 6 layers respectively Filtered through gauze.
Wherein, it is preferred that further include that the dry cream that will be obtained is placed on crushing in pulverizer, grinding, molecular sieve processing obtains institute State the dry powder of Chinese medicine composition.
Wherein, it is preferred that the molecular sieve processing refers to successively uses 10 mesh net filtrations, 100 mesh screen mistakes respectively Filter.
Further, the invention also provides the Chinese medicine compositions in preparation treatment urarthritis drug Application.
Compared to the prior art, the beneficial effects of the present invention are:
The invention proposes a kind of new Chinese medicine compositions and preparation method thereof for being used to treat urarthritis.In this Drug composition has effects that expelling wind and clearing away heat, damp dispelling and pain relieving, regulating qi and dredging collateral.Results of animal shows that the Chinese medicine composition can To improve arthroncus degree, the arthritis index in urarthritis mouse toes portion, gouty joint can also be improved The immune organ organ index and oxidation resistance of scorching mouse, can more improve urarthritis mouse toes portion IL-12, IL-1 β, IL-6, TNF-α albumen and gene level, while the Chinese medicine composition is to the dirty of urarthritis mouse important organ Device index and mouse weight illustrate that the traditional Chinese medicine composition for treating urarthritis is effective without influence, and the Chinese medicine composition It is without side-effects, new technological means is provided for treatment urarthritis.
Detailed description of the invention
Influence of Fig. 1 Chinese medicine composition to the Articular swelling in urarthritis mouse toes portion；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 2 Chinese medicine composition to urarthritis mouse toes portion arthritis index；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 3 Chinese medicine composition to urarthritis mouse immune organ organ index；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 4 Chinese medicine composition to urarthritis mouse toes portion T-AOC；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 5 Chinese medicine composition to urarthritis mouse toes portion SOD content；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 6 Chinese medicine composition to urarthritis mouse toes portion GSH-PX；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 7 Chinese medicine composition to urarthritis mouse toes portion MDA；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 8 Chinese medicine composition to urarthritis mouse toes portion IL-12；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Fig. 9 Chinese medicine composition to urarthritis mouse toes portion IL-6；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Figure 10 Chinese medicine composition to urarthritis mouse toes portion IL-1 β；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Figure 11 Chinese medicine composition to urarthritis mouse toes portion TNF-α；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Figure 12 Chinese medicine composition is opposite to urarthritis mouse toes portion IL-12, IL-6, IL-1 β, TNF-α gene The influence of expression quantity；
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05；
Influence of Figure 13 Chinese medicine composition to urarthritis mouse liver index, cardiac index, Lung Exponent；
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05；
Influence of Figure 14 Chinese medicine composition to urarthritis mouse cerebral index, renal index；
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05；
Influence of Figure 15 Chinese medicine composition to urarthritis mouse weight；
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05.
Specific embodiment
The present invention is further described with attached drawing combined with specific embodiments below, the advantages and features of the present invention will be with Description and it is apparent.But examples are merely exemplary for these, and it is not intended to limit the scope of the present invention in any way.This field Technical staff should be understood that without departing from the spirit and scope of the invention can details to technical solution of the present invention and Form is modified or is replaced, but these modifications and replacement are fallen within the protection scope of the present invention.
Embodiment 1 is a kind of for treating the preparation of the Chinese medicine composition of urarthritis
It is a kind of for treating the Chinese medicine composition of urarthritis, be made of each bulk pharmaceutical chemicals of following parts by weight: stone 6.5 parts by weight of nanmu leaf, 12 parts by weight of Pine Nodular Branch, 22.5 parts by weight of strophanthus divaricatus, 22.5 parts by weight of water banyan, 7.5 weight of lycopodium calvatum Part, 6.5 parts by weight of root of pungent litse fruit, 6 parts by weight of Fructus Aesculi, 6.8 parts by weight of rhizoma homalonemae.
Embodiment 2 is a kind of for treating the preparation of the Chinese medicine composition of urarthritis
It is a kind of for treating the Chinese medicine composition of urarthritis, be made of each bulk pharmaceutical chemicals of following parts by weight: stone 5 parts by weight of nanmu leaf, 14 parts by weight of Pine Nodular Branch, 20 parts by weight of strophanthus divaricatus, 25 parts by weight of water banyan, 6 parts by weight of lycopodium calvatum, Litsea pungens 8 parts by weight of root, 5 parts by weight of Fructus Aesculi, 7 parts by weight of rhizoma homalonemae.
Embodiment 3 is a kind of for treating the preparation of the Chinese medicine composition of urarthritis
It is a kind of for treating the Chinese medicine composition of urarthritis, be made of each bulk pharmaceutical chemicals of following parts by weight: stone 8 parts by weight of nanmu leaf, 10 parts by weight of Pine Nodular Branch, 25 parts by weight of strophanthus divaricatus, 20 parts by weight of water banyan, 9 parts by weight of lycopodium calvatum, Litsea pungens 5 parts by weight of root, 7 parts by weight of Fructus Aesculi, 5 parts by weight of rhizoma homalonemae.
Embodiment 4 is a kind of for treating the preparation of the Chinese medicine powder of urarthritis
(1) each bulk pharmaceutical chemicals are weighed according to following parts by weight:
6.5 parts by weight of folium photiniae, 22.5 parts by weight of strophanthus divaricatus, 22.5 parts by weight of water banyan, stretch muscle at 12 parts by weight of Pine Nodular Branch Careless 7.5 parts by weight, 6.5 parts by weight of root of pungent litse fruit, 6 parts by weight of Fructus Aesculi, 6.8 parts by weight of rhizoma homalonemae.
(2) each bulk pharmaceutical chemicals are put into container after mixing, 15 times of drug weight of distilled water is added, after impregnating 2h, 2h is decocted, it is cooling；
(2) step (1) medical fluid after cooling is successively used into 2 layers of filtered through gauze, 4 layers of filtered through gauze, 6 layers of gauze mistake respectively Filter, obtains first time filtrate and drug residue；
(3) the drug residue of step (2) is put into container, is repeated step (1) and (2) and is obtained second of filtrate, mixes the First-time filtrate and second of filtrate；
(4) the mixed liquor 6d for using drying box dry first time filtrate and second of filtrate at 65 DEG C, obtains in described The dry cream of drug composition；
(5) dry cream obtained, which is placed in pulverizer, repeatedly to be crushed, carefulness grinding, respectively successively using 10 mesh net filtrations, 100 mesh net filtrations, obtain the dry powder of the Chinese medicine composition.
The pharmacodynamic test of 5 traditional Chinese medicine composition for treating urarthritis of embodiment
1 material and method
1.1 experimental animal
4 monthly age male cleaning grade kunming mices 84, weight (23 ± 2) g, purchase are tested in Heilongjiang University of Chinese Medicine Animal center, the animal quality certificate of competency number SCXK (black) 2013-004.In Heilongjiang University of Chinese Medicine's Experimental Animal Center Interior raising kunming mice, kunming mice are raised using ordinary temperature, traditional humidity, and it is enough clear to need to give kunming mice Clean feed, drinking water, kunming mice freely obtain food, and 2/cage, the feeding work of kunming mice is big by Heilungkiang traditional Chinese medicine Experimental Animal Center professor Li Baolong is learned to complete.
1.2 experimental drug
Chinese medicine folium photiniae, Pine Nodular Branch, strophanthus divaricatus, water banyan, lycopodium calvatum, root of pungent litse fruit, Fructus Aesculi, rhizoma homalonemae are by Heilungkiang University of TCM Chinese medicine study institute professor Zhou Zhongguang buys in the attached First Hospital of Heilongjiang University of Chinese Medicine, according to 1989 Jiangsu Local standard is awarded by pharmaceutical college, Heilongjiang University of Chinese Medicine field penetrating judgment and is identified, qualification result meets regulation.It is not fast Purine alcohol, Chongqing in China Qingyang pharmaceutcal corporation, Ltd.PrimeRT reagent KIT, Japanese TaKaRa company.IL- 12, Bioengineering Research Institute is built up in IL-1 β, TNF-α, IL-6 enzyme linked immunological kit, Nanjing, and 20150501.Monosodium urate, the U.S. Sigma company.β-action, IL-12, IL-1 β, TNF-α, IL-6 primer, U.S. Santa Cruz Biotechnology are public Department.Bioengineering Research Institute 20150501 is built up in SOD, GSH-PX, T-AOC, MDA kit, Nanjing.Other reagents are domestic Analyze it is pure, by Qiqihar Medical College associate professor Zhang Yingbo provide.
1.3 laboratory apparatus
PLZOZ-S type electronic balance, Sai Duolisi company.6100 type microplate reader, RT company, the U.S..- 20 DEG C of refrigerators, -80 DEG C refrigerator, Electrical Appliances Co., Ltd, Chinese Qingdao Haier.TT30-FW-100 type high speed Universal pulverizer, the Wenling city big machinery of woods have Limit company.Rsearch UV type ultrapure water system, Shanghai Hitech Instruments Co., Ltd..Applied Biosystems type is quantitative PCR instrument, Applied biosystems.MU5100P type ultrapure water instrument, Beijing section praise industrial development in science and technology Co., Ltd. TGL-16G type desk centrifuge, Anting Scientific Instrument Factory, Shanghai.
The grouping of 1.4 experiment mices
Experiment mice is grouped at random according to table of random number, needs 84 kunming mices being divided into 7 groups, wherein yin Property control group 12, false injection group 12, model group 12, positive controls 12, Chinese medicine composition high dose group is (referred to as high Dosage group) 12, Chinese medicine composition middle dose group (abbreviation middle dose group) 12, (referred to as low dose of Chinese medicine composition low dose group Amount group) 12, every group of kunming mice is all made of picric acid solution and is marked, and kunming mice can carry out after adapting to raising 1 week Experiment, while needing to observe the general state of every group of mouse at any time, including kunming mice mobility, excreta, hair color, drink Food and its drinking-water etc..
1.5 experiment mice modelings
In general, it would generally be absorbed by organisms after 1w in lithate injector body, in order to maintain gouty to close The stability for saving scorching mouse model needs to be used for multiple times lithate for the same position of same mouse and carries out injection preparation gouty Arthritis model (Li H, Ou G, He Y, et al.Resveratrol attenuates the MSU crystal- induced inflammatory response through the inhibition of TAK1 activity[J].Int Immunopharmacol, 2019,67:62-68.；Qiu L, Tang CL, Huang SQ, et al.Effect of Electroacupuncture on Synovial M1/M2Macrophage Polarization in Rats with Acute Gouty Arthritis [J] .Zhen Ci Yan Jiu, 2018,43 (12): 767-72.).
(1) first time modeling
(Liu Juncheng, Zheng Deyong Sargent gloryvine polygonum cuspidate compound exempt from decoction treatment acute gouty arthritis to this laboratory reference document The Yunnan experimental study [J] traditional Chinese medicine magazine, 2018,39 (9): 75-76.；Jin Xiaomin is from NLRP3/ASC/Caspase-1 axis Mechanism [D] the Zhejiang University of Traditional Chinese Medicine of discussion smilax glabra total flavones prevention and treatment urarthritis, 2018.；Yang Q, Zhang Q, Qing Y, et al.miR-155 is dispensable in monosodium urate-induced gouty 2018,20 (1): inflammation in mice [J] .Arthritis Res Ther 144.) prepares urarthritis mould Type.After kunming mice conventinal breeding 1 week, observe that kunming mice general state is good, preference pattern group 12, positive controls 12, high dose group 12,12 middle dose group 12, low dose group kunming mices preparation urarthritis models, experiment Person carries out disinfection superclean bench, always wears your gloves, and prepares 2.5% uric acid sodium solution, grabs mouse, sterilizes kunming mice Right side lower limb, in addition experimenter foot bottom and its dorsal portion on the right side of kunming mice injects the urine of 0.05mL 2.5% respectively Acid sodium solution.False injection group 12 foot bottom and its dorsal portion on the right side of kunming mice is selected to inject 0.05mL physiological saline.Yin Property 12 kunming mices of control group do not inject uric acid sodium solution and physiological saline.
(2) second of modeling
7d after this experiment first time modeling success, according to the basic process (base of urarthritis model mice modeling This process is with first time modeling), again depending on bibliography, (it is acute that Liu Juncheng, Zheng Deyong Sargent gloryvine polygonum cuspidate compound exempt from decoction treatment The Yunnan experimental study [J] the traditional Chinese medicine magazine of urarthritis, 2018,39 (9): 75-76.；Yang Q, Zhang Q, Qing Y, et al.miR-155 is dispensable in monosodium urate-induced gouty 2018,20 (1): inflammation in mice [J] .Arthritis Res Ther 144.) prepares urarthritis mould Type.12 preference pattern group 12, positive controls 12, high dose group 12, middle dose group 12, low dose group Kunming are small Mouse, experimenter carry out disinfection superclean bench, always wear your gloves, and prepare 2.5% uric acid sodium solution, grab mouse, sterilize elder brother The right side lower limb of bright mouse, in addition experimenter foot bottom and its dorsal portion on the right side of kunming mice injects 0.05mL respectively 2.5% uric acid sodium solution.False injection group foot bottom and its dorsal portion on the right side of kunming mice is selected to inject 0.05mL physiology salt Water.12 kunming mices of negative control group do not inject uric acid sodium solution and physiological saline.
(3) third time modeling
14d after this experiment first time modeling success, according to the basic process (base of urarthritis model mice modeling This process is with first time modeling, second of modeling) preparation urarthritis model.Preference pattern group 12, positive controls 12, high dose group 12,12 middle dose group 12, low dose group kunming mices, experimenter disappear superclean bench Poison always wears your gloves, and prepares 2.5% uric acid sodium solution, grabs mouse, sterilizes the right side lower limb of kunming mice, in addition a reality The person of testing foot bottom and its dorsal portion on the right side of kunming mice inject the uric acid sodium solution of 0.05mL2.5% respectively.The false injection of selection Group foot bottom and its dorsal portion on the right side of kunming mice inject 0.05mL physiological saline.12 kunming mices of negative control group are not infused Penetrate uric acid sodium solution and physiological saline.
The evaluation of 1.6 mouse models
(1) first time modeling is evaluated
After first time modeling terminates 6h, model group 12, positive controls 12, high dose group 12, middle dose group 12 Only, 12 kunming mices of low dose group are showed no death, and kunming mice toes portion obvious tumefaction is oozed out without liquid；It ties simultaneously It closes false injection 12 kunming mices of group and has no dead, the portion's swelling of kunming mice toes is unobvious, oozes out without liquid；Negative control 12 kunming mices of group have no dead, and toes portion is normal.The above prompts urarthritis mouse model modeling success, can To carry out subsequent experimental study.
(2) second of modeling evaluation
After second of modeling terminates 6h, model group 12, positive controls 12, high dose group 12, middle dose group 12 Only, 12 kunming mices of low dose group are showed no death, and kunming mice toes portion obvious tumefaction is oozed out without liquid；It ties simultaneously It closes false injection 12 kunming mices of group and has no dead, the portion's swelling of kunming mice toes is unobvious, oozes out without liquid；Negative control 12 kunming mices of group have no dead, and toes portion is normal.The above prompts urarthritis mouse model modeling success, can To carry out subsequent experimental study.
(3) third time modeling is evaluated
It is model group dead 2,10 remaining after third time modeling terminates 6h, it is positive controls dead 1,11 remaining Mouse；High dose group dead 1, remaining 11 mouse；Middle dose group dead 1, remaining 11 mouse；It is low dose group 12 small Mouse has no dead, and each group kunming mice toes portion obvious tumefaction is oozed out without liquid；Dead 1 in combination with false injection group, remain Remaining 11 mouse, the portion's swelling of kunming mice toes is unobvious, oozes out without liquid；12 kunming mices of negative control group have no dead It dies, toes portion is normal.The above prompts urarthritis mouse model modeling success, can carry out subsequent experiment and grind Study carefully.
1.7 Chinese medicine compositions extract
(1) purchase and drug composition
This experiment need to buy every part by folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, stretch muscle The Chinese medicine composition that careless 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g are formed, buys altogether 5 parts.
(2) every part of Chinese medicine composition dosage is calculated
Every part of Chinese medicine composition includes folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g amount to 90.3g.By Heilongjiang University of Chinese Medicine Li Ming secondary master After appointing evaluation, this every part 90.3g Chinese medicine composition dosage is the convention security dosage of normal body.
(3) 5 parts of Chinese medicine compositions are prepared
It needs to take folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, wood respectively Each 5 parts of Jiang Zigen 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g, 5 parts of Chinese medicine compositions are prepared, are obtained by folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g are formed Chinese medicine composition, 5 parts of Chinese medicine compositions amount to 451.5g.
(4) Chinese medicine composition first time filtrate is obtained
It chooses 451.5g Chinese medicine composition to be put into 20000mL large capacity round-bottomed flask, distilled water 7000mL is added, needs Distilled water immersion 451.5g Chinese medicine composition 2h decocts 451.5g Chinese medicine composition 2h, 2 layers of filtered through gauze, 4 layers of yarn at 100 DEG C After cloth filtering, 6 layers of filtered through gauze, first time filtrate and the drug residue of 451.5g Chinese medicine composition are obtained.
(5) second of filtrate of Chinese medicine composition is obtained
The residue of 451.5g Chinese medicine composition is put into 20000mL large capacity round-bottomed flask, distilled water 6000mL is added, Distilled water immersion Chinese medicine composition residue 2h, decocting herb composition residue 2h at 100 DEG C, 2 layers of filtered through gauze, 4 layers of gauze mistake After filter, 6 layers of filtered through gauze, second of filtrate of Chinese medicine composition is obtained.
(6) Chinese medicine composition dry cream is obtained
Merge first time filtrate and its second of filtrate, dries first time filtrate and its second at 65 DEG C using drying box Secondary filtrate 6d is obtained and is mentioned containing folium photiniae, Pine Nodular Branch, strophanthus divaricatus, water banyan, lycopodium calvatum, root of pungent litse fruit, Fructus Aesculi, rhizoma homalonemae Taking the Chinese medicine composition dry cream of object, PLZOZ-S type electronic balance (Sai Duolisi company) is weighed Chinese medicine composition dry cream 85.6g, Then the Chinese medicine composition paste-forming rate is (dry cream/Chinese medicine composition) × 100%, i.e. (85.6g/451.5g) × 100%= 18.96%, i.e. 1g Chinese medicine composition dry cream is equivalent to Chinese medicine composition crude drug 5.27g.
(7) Chinese medicine composition dry powder is obtained
The Chinese medicine composition dry cream of acquisition is repeatedly crushed using pulverizer, carefulness grinding, 10 mesh net filtrations, 100 mesh The screen to filtrate filtering, PLZOZ-S type electronic balance (Sai Duolisi company) weighing, obtains Chinese medicine composition dry powder 81.4g, then should Chinese medicine composition flour extraction=(dry powder/Chinese medicine composition) × 100%=(81.4g/451.5g) × 100%=18.03%.
(8) Chinese medicine composition dry powder is saved
Experiment uses 451.5g Chinese medicine composition, final to obtain Chinese medicine composition dry powder 81.4g, i.e. 1g Chinese medicine composition is dry Powder is equivalent to Chinese medicine composition crude drug 5.55g.
(9) Chinese medicine composition dry powder is dispensed
The Chinese medicine composition dry powder is dispensed according to 10g/ bags, last bag of 11.4g, totally 8 bags, -80 DEG C of refrigerators save standby With.It needs to melt in the Chinese medicine composition room temperature when using Chinese medicine composition dry powder, after stirring, and according to the body surface face of mouse Long-pending and its weight numerical value finely converts mouse Chinese medicine composition dosage.
1.8 setting drug doses
(1) Chinese medicine composition dosage is set
The safe dose pharmaceutical composition of every part of Chinese medicine composition include folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, Water banyan 22.5g, lycopodium calvatum 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g, 90.3g altogether will be according to mouse It converts with body surface area's ratio 0.0026:1, calculating is acquired containing folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, the Chinese medicine composition of rhizoma homalonemae 6.8g are conventional Dosage is [(90.3g × 0.0026)/20g mouse] × 50, i.e. 11.74g/kg.This be containing folium photiniae 6.5g, Pine Nodular Branch 12g, The Chinese traditional medicine composition of strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, root of pungent litse fruit 6.5g, Fructus Aesculi 6g, rhizoma homalonemae 6.8g Object middle dosage.Final setting is by folium photiniae 6.5g, Pine Nodular Branch 12g, strophanthus divaricatus 22.5g, water banyan 22.5g, lycopodium calvatum 7.5g, wood Jiang Zigen 6.5g, Fructus Aesculi 6g, the Chinese medicine composition high dose of rhizoma homalonemae 6.8g composition, Chinese medicine composition middle dosage, Chinese medicine group Closing object low dosage is 23.48g/kg, 11.74g/kg, 5.87g/kg respectively.
(2) positive controls drug dose is set
Clinically the routine of allopurinol treatment urarthritis can be (200~400) mg with dosage.This experiment needs Stringent foundation mouse and body surface area's ratio are 0.0026:1 calculating, and calculating and obtaining mouse dosage is [200 × 0.0026 × 50~400 × 0.0026 × 50], i.e. (26~52) mg/kg or (0.026~0.052) g/kg, this experimental selection 0.04g/ Kg allopurinol carries out experimental study.
The administration of 1.9 experiment mices
After first time modeling terminates 7h, each group mouse can be administered.Wherein, negative control group kunming mice is given 0.4mL physiological saline stomach-filling 21d；False injection group kunming mice gives 0.4mL physiological saline stomach-filling 21d；Model group kunming mice Give 0.4mL physiological saline stomach-filling 21d；Positive controls give 0.04g/kg allopurinol stomach-filling 21d；High dose group is given 23.48g/kg Chinese medicine composition (being equivalent to Chinese medicine composition crude drug) stomach-filling 21d；Middle dose group gives 11.74g/kg Chinese medicine Composition (being equivalent to Chinese medicine composition crude drug) stomach-filling 21d；Low dose group gives 5.87g/kg Chinese medicine composition (in being equivalent to Drug composition crude drug) stomach-filling 21d.After this experiment gastric infusion, each group mouse survival number includes negative control group 12 The survival of kunming mice；The 11 kunming mice survivals of false injection group；10 kunming mice survivals of model group；Positive controls 11 Kunming mice survival；11 kunming mice survivals of high dose group；11 kunming mice survivals of middle dose group；12 elder brothers of low dose group Bright mouse survival.
1.10 measurement toes portion Articular swellings
(Li Baolong, Du Hui, Wu Juan wait a kind of for treating the Chinese traditional medicine composition of urarthritis to this laboratory reference document The Heilungkiang object and its preparation method and application [P]: CN104800471A, 2015-07-29.；Zhou Zhongguang, Du Xin, Tian Jiaqi, A kind of equal Chinese medicine composition for preventing and treating urarthritis of and its preparation method and application the Heilungkiang [P]: CN104825715A, 2015-08-12.) measurement each group mouse toes portion Articular swelling.In every kunming mice before experiment Knee joint knee with marking pen mark horizontal line.Mouse is put in by this experiment after last time gastric infusion terminates 2h In closed glass container, ether is injected into glass container, starts anesthetized mice, while observing the basic shape of kunming mice State avoids anesthesia excessive.After mouse anesthesia is good, mouse toes portion is cut from knee bend mark, is put in equipped with pre-cooling life It manages in salt water graduated cylinder, the volume scale in accurate record observation mouse toes portion.
The calculation formula of toes portion Articular swelling are as follows: mouse toes portion is closed after toes portion Articular swelling (mL)=experiment Save mouse toes portion joint solvent (mL) before solvent (mL)-is tested
1.11 measurement toes portion arthritis indexes
(Zhou Youliang exempts from the numbness joint-running side's Hunan anti-inflammatory and antalgic mechanism experimental study [D] traditional Chinese medicine clearly to this laboratory reference document University, 2017.；Influence and its autophagy mechanism research [D] Hubei Chinese medicine of the Li Xiaojuan mild-warm moxibustion to mouse bacteria infection inflammation Medicine university, 2014.) it is measured urarthritis toes portion arthritis index.Urarthritis toes portion arthritis Disease index includes 4 grades, wherein 0 grade 0 point, 1 grade 1~2 point, 2 grades 3~4 points, 3 grades 5~6 points.0 grade of expression mouse toes portion does not have Red, swollen, hot, the bone mark in toes portion is high-visible, illustrates that toes portion structure is normal；There is limitation in 1 grade of mouse toes portion Swelling, joint appearance is red, hot, and the bone mark in toes portion can't see, and illustrate that mild swelling occurs in mouse toes portion；2 grades of mouse There is globality swelling in toes portion, and red, hot, pain occurs in joint, and toes portion bone mark can't see, and illustrates mouse toes portion moderate Swelling；There is globality swelling in 3 grades of mouse toes portions, and red, hot, pain occurs in joint, and toes portion bone mark can't see appearance, together When toes portion other than joint part illustrate the severe swelling of mouse toes portion if obvious tumefaction occurs in knee joint.This experiment needs Each group mouse toes portion's arthritis index score values at different levels are recorded, standardization statistical analysis below is then carried out.This experiment exists After last time gastric infusion terminates 2h, each group mouse toes portion is observed, evaluates each group mouse toes portion arthritis index.
1.12 measurement mice organs indexes
(Li Weixia, Wang Yanfang, Liu Xiaoxia wait black rice and whortle anthocyanin to mouse constitution to this laboratory reference document [J] China medicine company, 2018,27 (22): 8-10. are compared in the influence of amount and organ index) measurement each group mice organs index.? After last time gastric infusion terminates 2h, sacrificed by decapitation mouse dissects mouse, draw materials mice spleen, thymus gland, brain, the heart, lung, kidney, liver Equal internal organs, weighing refer to according to the internal organs that organ index formula calculates the internal organs such as each group mice spleen, thymus gland, brain, the heart, lung, kidney, liver Number.
The calculation formula of organ index are as follows: organ index (mg/g)=Organ weight (mg)/mouse weight (g)
1.13 measurement toes portion oxidation resistances
This experiment measures the oxidation resistance in each group mouse toes portion using enzyme-linked immunosorbent assay method.At last After secondary gastric infusion terminates 2h, 10% chloral hydrate anesthesia mouse, draw materials rapidly mouse toes portion on ice, removes skin, essence Fine lapping, homogenate, 4 DEG C of standing homogenate 1h stand 1h, and TGL-16G type desk centrifuge 3000rpm/min is centrifuged 20min, takes Each group mouse toes portion supernatant, is dispensed, according to kit specification measurement each group mouse toes portion T-AOC, SOD, GSH-PX, MDA content
1.14 measurement toes portion inflammatory mediator protein expressions
(Huang Jing, Li Ang, Liu Yuguang wait the white rake teeth bacteria solid fermentation object of to hyperuricemia and pain to this laboratory reference document The northwest wind arthritic therapeutic effect [J] pharmaceutical journal, 2018,33 (4): 488-491.；Zhou Q, Lin FF, Liu SM, et al.Influence of the total saponin fraction from Dioscorea nipponica Makino on TLR2/4-IL1R receptor singnal pathway in rats of gouty arthritis[J] .J Ethnopharmacol, 2017,12 (206): 274-282.；Wang Ping, Sun Tiefeng, state's brocade wait the sharp relaxation grain treatment of pain anxious Property urarthritis the Shandong experimental study [J] science, 2018,31 (6): 18-23.), use enzyme-linked immunosorbent assay The inflammatory mediator protein expression level of method measurement each group mouse.After last time gastric infusion terminates 2h, need to grab small Mouse, anesthetized mice, draw materials rapidly mouse toes portion on ice, removes skin, and fine gtinding is homogenized, 4 DEG C of standing homogenate 1h, TGL-16G type desk centrifuge 3000rpm/min is centrifuged 20min, takes supernatant, dispenses, and detects each group mouse toes portion joint IL-12, IL-6, IL-1 β, TNF-α protein content are organized, it is spare that remaining sample is stored in -80 DEG C of refrigerators.Illustrate according to kit Book, using absorbance value (OD) at 6100 type microplate reader measurement 450nm wavelength, according to calibration curve equation and each hole absorbance value (OD) mouse toes portion joint tissue inflammatory mediator IL-12, IL-6, IL-1 β, TNF-α content are calculated.
1.15 measurement toes portion inflammatory mediator gene expressions
This laboratory reference document (Zhang QB, Qing YF, Yin CC, et al.Mice with miR-146a deficiency develop severe gouty arthritis via dysregulation of TRAF 6 IRAK 1 And NALP3 inflammasome [J] .Arthritis Res Ther, 2018,20 (1): 45.), uses Applied Biosystems type quantitative PCR apparatus measures each group mouse toes portion joint tissue inflammatory mediator IL- using Real-time-PCR 12, the expression of IL-1 β, IL-6, TNF-α mRNA.After last time gastric infusion terminates 2h, need fresh mouse toes portion RNA, and reverse transcription designs each group mouse toes portion joint tissue inflammatory mediator IL-12, IL-1 β, IL-6, TNF-α at cDNA The specific primer of mRNA.Experiment needs plus special SYBR Green I fluorescent material, and setting parameter includes 50 DEG C of 2min, and 95 DEG C 10min, 95 DEG C of 15s, 60 DEG C of 1min, 40Cycles, using 2-ΔΔCtMethod calculates, and analytical calculation mouse toes portion joint tissue is scorching Disease medium IL-12, IL-1 β, IL-6, TNF-α mrna expression amount.
1.16 measuring mouse weight
(Zhou Zhongguang, ugly solar month of 30 days, Fei Hongxin wait pellet small stream gout side to chmice acute gouty joint to this laboratory reference document Scorching experimental study [J] traditional Chinese medicine journal, 2015,43 (1): 59-62.；Fei Hongxin, Han Yusheng, Liao Ting wait semen plantaginis to small The Heilungkiang influence [J] the science of mouse acute gouty arthritis, 2014,5 (5): 9-11.) it is small using electronic balance measurement each group Mouse weight.This experiment in 0d, 7d, 14d, 21d, measures each group mouse weight during the administration respectively, and statistics each group mouse exists 0d, 7d, 14d, 21d weight data change in administration process, it will evaluation Chinese medicine composition is to urarthritis model mice Side effect.
1.17 statistical analysis
Using SPSS19.0 software analysis data, data are usedIt indicates, more comparison among groups are using single factor test variance point Analysis, indicates that difference has statistical significance using P < 0.05, indicates that difference does not have statistical significance using P > 0.05.
2 results
Influence of 2.1 Chinese medicine compositions to the Articular swelling in urarthritis mouse toes portion
The experimental results showed that compared with negative control group, the joint in model group urarthritis model mice toes portion Swelling obviously increases (P < 0.05), and difference has statistical significance, and the Articular swelling in false injection group mouse toes portion changes Unobvious (P > 0.05), difference do not have statistical significance；Compared with model group, positive controls, high dose group, middle dosage The Articular swelling in group urarthritis model mice toes portion is substantially reduced (P < 0.05), and difference has statistical significance, And the Articular swelling in low dose group urarthritis model mice toes portion changes unobvious (P > 0.05), difference does not have It is statistically significant.It is shown in Table 1, Fig. 1.
Influence of 1 Chinese medicine composition of table to the Articular swelling in urarthritis model mice toes portion
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.2 Chinese medicine compositions to urarthritis mouse toes portion arthritis index
The experimental results showed that compared with negative control group, model group urarthritis model mice toes portion arthritis Disease index obviously increases (P < 0.05), and difference has statistical significance, and false injection group mouse toes portion's arthritis index changes Unobvious (P > 0.05), difference do not have statistical significance；Compared with model group, positive controls, high dose group, middle dosage Group urarthritis model mice toes portion arthritis index is substantially reduced (P < 0.05), and difference has statistical significance, And low dose group urarthritis model mice toes portion's arthritis index changes unobvious (P > 0.05), difference does not have It is statistically significant.It is shown in Table 2, Fig. 2.
Influence of 2 Chinese medicine composition of table to urarthritis model mice toes portion arthritis index
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.3 Chinese medicine compositions to urarthritis mouse immune organ organ index
The experimental results showed that compared with negative control group, model group urarthritis model mice immune organ internal organs Index obviously increases (P < 0.05), and difference has statistical significance, and false injection group mouse immune organ organ index changes unknown Aobvious (P > 0.05), difference do not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group pain Wind arthritis model mouse immune organ organ index is substantially reduced (P < 0.05), and difference has a statistical significance, and low dose Amount group urarthritis model mice immune organ organ index changes unobvious (P > 0.05), and difference does not have statistics Meaning.It is shown in Table 3, Fig. 3.
3 Chinese medicine composition of table to urarthritis model mice immune organ organ index influence (N=9)
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.4 Chinese medicine compositions to urarthritis mouse toes portion T-AOC
The experimental results showed that model group urarthritis model mice toes portion T-AOC is bright compared with negative control group Aobvious to reduce (P < 0.05), difference has statistical significance, and false injection group mouse toes portion T-AOC changes unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group gouty are closed Section inflammation model mice toes portion T-AOC is obviously increased (P < 0.05), and difference is with statistical significance, and low dose group gouty Arthritis model mouse toes portion T-AOC changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 4, Fig. 4.
Influence of 4 Chinese medicine composition of table to urarthritis model mice toes portion T-AOC
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.5 Chinese medicine compositions to urarthritis mouse toes portion SOD
The experimental results showed that compared with negative control group, model group urarthritis model mice toes portion SOD content It is substantially reduced (P < 0.05), difference has statistical significance, and false injection group mouse toes portion's SOD content changes unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group gouty are closed It saves scorching model mice toes portion SOD content to obviously increase (P < 0.05), difference has a statistical significance, and low dose group gout Property arthritis model mouse toes portion's SOD content change unobvious (P > 0.05), difference do not have statistical significance.Be shown in Table 5, Fig. 5.
Influence of 5 Chinese medicine composition of table to urarthritis model mice toes portion SOD content
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.6 Chinese medicine compositions to urarthritis mouse toes portion GSH-PX
The experimental results showed that compared with negative control group, model group urarthritis model mice toes portion GSH-PX Content is substantially reduced (P < 0.05), and difference has statistical significance, and false injection group mouse toes portion's GSH-PX content changes unknown Aobvious (P > 0.05), difference do not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group pain Wind arthritis model mouse toes portion GSH-PX content obviously increases (P < 0.05), and difference has a statistical significance, and low dose Amount group urarthritis model mice toes portion's GSH-PX content changes unobvious (P > 0.05), and difference does not have statistics Meaning.It is shown in Table 6, Fig. 6.
Influence of 6 Chinese medicine composition of table to urarthritis model mice toes portion GSH-PX
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.7 Chinese medicine compositions to urarthritis mouse toes portion MDA
The experimental results showed that model group urarthritis model mice toes portion MDA is obvious compared with negative control group Increase (P < 0.05), difference has statistical significance, and false injection group mouse toes portion MDA changes unobvious (P > 0.05), poor It is different not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group urarthritis model Mouse toes portion MDA is substantially reduced (P < 0.05), and difference has statistical significance, and low dose group urarthritis model Mouse toes portion MDA changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 7, Fig. 7.
Influence of 7 Chinese medicine composition of table to urarthritis model mice toes portion MDA
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.8 Chinese medicine compositions to urarthritis mouse toes portion IL-12
The experimental results showed that model group urarthritis model mice toes portion IL-12 is bright compared with negative control group Aobvious to increase (P < 0.05), difference has statistical significance, and false injection group mouse toes portion IL-12 changes unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group gouty are closed Section inflammation model mice toes portion IL-12 is substantially reduced (P < 0.05), and difference is with statistical significance, and low dose group gouty Arthritis model mouse toes portion IL-12 changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 8, Fig. 8.
Influence of 8 Chinese medicine composition of table to urarthritis model mice toes portion IL-12
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.9 Chinese medicine compositions to urarthritis mouse toes portion IL-6
The experimental results showed that model group urarthritis model mice toes portion IL-6 is bright compared with negative control group Aobvious to increase (P < 0.05), difference has statistical significance, and false injection group mouse toes portion IL-6 changes unobvious (P > 0.05), Difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group urarthritis mould Type mouse toes portion IL-6 is substantially reduced (P < 0.05), and difference has statistical significance, and low dose group urarthritis mould Type mouse toes portion IL-6 changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 9, Fig. 9.
Influence of 9 Chinese medicine composition of table to urarthritis model mice toes portion IL-6
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.10 Chinese medicine compositions to urarthritis mouse toes portion IL-1 β
The experimental results showed that model group urarthritis model mice toes portion IL-1 β is bright compared with negative control group Aobvious to increase (P < 0.05), difference has statistical significance, and false injection group mouse toes portion IL-1 β changes unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group gouty are closed Section inflammation model mice toes portion IL-1 β is substantially reduced (P < 0.05), and difference is with statistical significance, and low dose group gouty Arthritis model mouse toes portion IL-1 β changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 10, figure 10。
Influence of 10 Chinese medicine composition of table to urarthritis model mice toes portion IL-1 β
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 2.11 Chinese medicine compositions to urarthritis mouse toes portion TNF-α
The experimental results showed that model group urarthritis model mice toes portion TNF-α is bright compared with negative control group Aobvious to increase (P < 0.05), difference has statistical significance, and false injection group mouse toes portion's TNF-α changes unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group gouty are closed It saves scorching model mice toes portion TNF-α to be substantially reduced (P < 0.05), difference has a statistical significance, and low dose group gouty Arthritis model mouse toes portion's TNF-α changes unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 11, figure 11。
Influence of 11 Chinese medicine composition of table to urarthritis model mice toes portion TNF-α
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
2.12 Chinese medicine compositions are opposite to urarthritis mouse toes portion IL-12, IL-6, IL-1 β, TNF-α gene The influence of expression quantity
The experimental results showed that compared with negative control group, model group urarthritis model mice toes portion IL-12, IL-6, IL-1 β, TNF-α gene relative expression quantity obviously increase (P < 0.05), and difference has statistical significance, and false injection group is small Mouse toes portion IL-12, IL-6, IL-1 β, TNF-α gene relative expression quantity change unobvious (P > 0.05), and difference does not have system Meter learns meaning；Compared with model group, positive controls, high dose group, middle dose group urarthritis model mice toes portion IL-12, IL-6, IL-1 β, TNF-α gene relative expression quantity are substantially reduced (P < 0.05), and difference has statistical significance, and low Dosage group urarthritis model mice toes portion IL-12, IL-6, IL-1 β, the change of TNF-α gene relative expression quantity are unknown Aobvious (P > 0.05), difference do not have statistical significance.It is shown in Table 12, Figure 12.
12 Chinese medicine composition of table is to urarthritis model mice toes portion IL-12, IL-6, IL-1 β, TNF-α gene Relative expression quantity influence (N=9)
Note: compared with negative control group:*P > 0.05,**P < 0.05；Compared with model group:#P < 0.05,##P > 0.05
Influence of 3.13 Chinese medicine compositions to urarthritis mouse liver index, cardiac index, Lung Exponent
The result shows that compared with negative control group, model group urarthritis mouse liver index, cardiac index, Lung Exponent Change unobvious (P > 0.05), difference does not have statistical significance, and false injection group mouse liver index, cardiac index, Lung Exponent change Unobvious (P > 0.05), difference do not have statistical significance；Compared with model group, positive controls, high dose group, middle dosage Group, low dose group mouse liver index, cardiac index, Lung Exponent change unobvious (P > 0.05), and difference does not have statistical significance. It is shown in Table 13, Figure 13.
13 Chinese medicine composition of table to urarthritis mouse liver index, cardiac index, Lung Exponent influence (N=9)
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05
Influence of 3.14 Chinese medicine compositions to urarthritis mouse cerebral index, renal index
The result shows that model group urarthritis mouse cerebral index, renal index change unknown compared with negative control group Aobvious (P > 0.05), difference do not have statistical significance, and false injection group mouse cerebral index, renal index change unobvious (P > 0.05), difference does not have statistical significance；Compared with model group, positive controls, high dose group, middle dose group, low dose group Mouse cerebral index, renal index change unobvious (P > 0.05), and difference does not have statistical significance.It is shown in Table 14, Figure 14.
Influence of 14 Chinese medicine composition of table to urarthritis mouse cerebral index, renal index
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05
Influence of 3.15 Chinese medicine compositions to urarthritis mouse weight
The result shows that compared with control group 0d, 7d, 14d, 21d mouse weight, model group 0d, 7d, 14d, 21d Mice Body Change again unobvious (P > 0.05), difference does not have statistical significance, and false injection group 0d, 7d, 14d, 21d mouse weight changes Unobvious (P > 0.05), difference do not have statistical significance；It is positive right compared with model group 0d, 7d, 14d, 21d mouse weight Change unobvious (P > 0.05) according to group, high dose group, middle dose group, low dose group 0d, 7d, 14d, 21d mouse weight, difference Without statistical significance.It is shown in Table 15, Figure 15.
15 Chinese medicine composition of table to urarthritis mouse weight influence (N=9)
Note: compared with negative control group:*P > 0.05；Compared with model group:**P > 0.05.
4, conclusion
The above experiment shows that Chinese medicine composition height, middle dosage (23.48g/kg, 11.74g/kg) can improve gouty pass Arthroncus degree, the arthritis index for saving scorching mouse toes portion, can also improve the immune device of urarthritis mouse Official's organ index and oxidation resistance, can more improve the IL-12 in urarthritis mouse toes portion, IL-1 β, IL-6, TNF-α albumen and gene level, while the Chinese medicine composition is to the organ index of urarthritis mouse important organ and small Mouse weight illustrates that the traditional Chinese medicine composition for treating urarthritis is effective without influence, and the Chinese medicine composition is without side-effects.

Claims (9)

1. a kind of for treating the Chinese medicine composition of urarthritis, which is characterized in that the Chinese medicine composition is by following Each bulk pharmaceutical chemicals of parts by weight form: folium photiniae 5-8 parts by weight, Pine Nodular Branch 10-15 parts by weight, strophanthus divaricatus 20-25 parts by weight, water father-in-law Flower 20-25 parts by weight, lycopodium calvatum 6-9 parts by weight, root of pungent litse fruit 5-8 parts by weight, Fructus Aesculi 5-7 parts by weight, rhizoma homalonemae 5-8 weight Part.
2. as described in claim 1 a kind of for treating the Chinese medicine composition of urarthritis, which is characterized in that described Chinese medicine composition is made of each bulk pharmaceutical chemicals of following parts by weight: 6.5 parts by weight of folium photiniae, 12 parts by weight of Pine Nodular Branch, strophanthus divaricatus 22.5 parts by weight, 22.5 parts by weight of water banyan, 7.5 parts by weight of lycopodium calvatum, 6.5 parts by weight of root of pungent litse fruit, 6 parts by weight of Fructus Aesculi, 6.8 parts by weight of rhizoma homalonemae.
3. a kind of Chinese materia medica preparation for being used to treat urarthritis for being clinically suitable for, it is characterised in that by claims 1 or 2 Auxiliary material needed for preparations shaping is added in the Chinese medicine composition, is made according to the conventional method for preparing pharmaceutical preparation.
4. Chinese materia medica preparation as claimed in claim 3, it is characterised in that the preparation is water decoction, pulvis, capsule, ball Agent, granule, tablet or oral solution.
5. a kind of method for preparing Chinese medicine composition of any of claims 1 or 2, it is characterised in that the following steps are included:
(1) parts by weight according to claim 1 or 2 weigh each bulk pharmaceutical chemicals, and each bulk pharmaceutical chemicals are put into container after mixing In, 10-20 times of drug weight of distilled water is added, after impregnating 1-2.5h, decocts 1-2.5h, it is cooling；
(2) by step (1) medical fluid filtered through gauze after cooling, first time filtrate and drug residue are obtained；
(3) the drug residue of step (2) is put into container, is repeated step (1) and (2) and is obtained second of filtrate, mixing is for the first time Filtrate and second of filtrate；
(4) dry, obtain the dry cream of the Chinese medicine composition.
6. method as claimed in claim 5, which is characterized in that the filtered through gauze refers to successively uses 2 layers of gauze respectively Filtering, 4 layers of filtered through gauze, 6 layers of filtered through gauze.
7. method as claimed in claim 5, which is characterized in that further include that the dry cream that will be obtained is placed on crushing in pulverizer, grind Mill, molecular sieve processing, obtains the dry powder of the Chinese medicine composition.
8. method as claimed in claim 5, which is characterized in that the molecular sieve processing refers to successively uses 10 meshes respectively Net filtration, 100 mesh net filtrations.
9. application of the Chinese medicine composition of any of claims 1 or 2 in preparation treatment urarthritis drug.
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CN114272343A (en) * 2021-12-24 2022-04-05 黑龙江儒泰科技发展有限责任公司 Traditional Chinese medicine composition for treating female gouty arthritis and preparation method and application thereof

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