CN102860218A - Field cultivation method for grassland white mushrooms - Google Patents

Field cultivation method for grassland white mushrooms Download PDF

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CN102860218A
CN102860218A CN2012103742983A CN201210374298A CN102860218A CN 102860218 A CN102860218 A CN 102860218A CN 2012103742983 A CN2012103742983 A CN 2012103742983A CN 201210374298 A CN201210374298 A CN 201210374298A CN 102860218 A CN102860218 A CN 102860218A
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prairie tricholomataceae
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CN102860218B (en
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徐国元
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Abstract

The invention discloses a field cultivation method for grassland white mushrooms. By the field cultivation method, the grassland white mushrooms can be cultivated artificially in the original ecological environment. By artificially cultivating and rejuvenating stock culture, cultivating stock seeds and cultivar, preparing cultivation materials according to biological characteristics of the grassland white mushrooms, obeying geoherbalism of the grassland white mushrooms and massively producing the grassland mushrooms by means of physioclimate, vegetation conditions and auxiliary manual intervention measures in the original ecological area of the grassland white mushrooms, the field cultivation method realizes artificial cultivation of endangered grassland white mushrooms and keeps rich nutritions, edible value of fresh taste and thick fragrance and medical and healthcare values of the grassland white mushrooms. Without applying chemical fertilizer and pesticides, ecology of grasslands is protected from damage, natural pollution-free wild characteristics of the grassland white mushrooms are kept, and the ecological environment of the grasslands is protected.

Description

The prairie Tricholomataceae field planting method
Technical field
The present invention relates to wild edible fungus culture technique field, particularly a kind of prairie Tricholomataceae field planting method.
Background technology
Prairie Tricholomataceae has another name called Mongolian dried mushroom (tricholoma mongolicum Imai), and to belong to Agaricales (A garicales), Tricholomataceae (Tricholomat aceae), Tricholoma (Tricholoma) be the northerly edible mushroom of growth.
Prairie Tricholomataceae contains the mineral elements such as rich in protein, vitamin and potassium, selenium, iron, phosphorus and polysaccharide, polypeptide, triterpene isoreactivity composition, have high edibility and higher medicinal, health-care effect, mainly be distributed in grassland, the Qiu Ling area in the Inner Mongol, Hebei, Heilungkiang, Jilin.
The bacterial context of prairie Tricholomataceae is plump, quality is fine and smooth, strongly fragrant just aromatic, and taste is unique delicious, is treasure in the bacterium, and the market that always enjoys a good market both at home and abroad is the domestic fungus resource of China's preciousness.But in recent years because ecological disruption, grass-land deterioration and gather the reasons such as excessive, output declines to a great extent, and this precious wild-type strain of prairie Tricholomataceae is endangered.
The endangered problem of prairie Tricholomataceae obtains relevant expert scholar's concern in recent years, begin growth conditions and the ecotope of prairie Tricholomataceae are investigated, and obtained a collection of achievement, also carried out research and the test of prairie Tricholomataceae artificial cultivation technique, broken through but not yet obtain at present essence.
Prairie Tricholomataceae is the northerly wild edible fungus of growth, the biological characteristic with following uniqueness:
1, Regional Distribution, prairie Tricholomataceae mainly are distributed in grassland, the Qiu Ling area that economize in the Inner Mongol, Hebei, Heilungkiang, Jilin etc., in Mongolia, Russia distribution are arranged also.
2, geography and climate characteristics, prairie Tricholomataceae suitable growth area height above sea level 600-1700m, average temperature of the whole year-3-1 ℃, more than or equal to 10 ℃ of accumulated temperature at 1800-2600 ℃, about frostless number of days 90-110 days, annual precipitation 300-600 millimeter, wherein 50% concentrate on the 7-8 month, year, gentle degree was 0.3-0.7, and atmospheric moisture 4-7mb has the hot same period of rain and the large characteristics of day and night temperature.
3, prairie Tricholomataceae mycelial growth developmental characteristic, surviving the winter in the mycelia field can be-45 ℃ of survival, go up begin recover growth with temperature the annual the first tenday period of a month in May, the growth of 5-20cm depths spreads under soil, July first arrival early August forms velvet-like mycelia and former base in soil 3-10cm depths, late July to early September produces fruit body, and early August to early September is the multiple phase of fruit body.
4, soil types, the soil types of prairie Tricholomataceae growth is chernozem, chestnut soil, P in soil H value 5.6-7.2, water content 25-40%, the withered layer of soil table herbage 3-5cm is in humus layer 30-40cm, the root system of plant humus layer more than 25cm.
5, vegetation structure, the prairie Tricholomataceae vegetation distributes with distinctive fairy ring type, plant has diversity and complexity, take rhizome grass and tussock as main, constructive species is sheep's hay, the main plant kind comprises the compositions such as sheep's hay, Bei Jiaer pin Acorns, wheatgrass, hidden son grass, day cloudy villous themeda, prairie sagewort, Da grass, Filifolium sibiricum, sheep Acorns, yellow capsule sedge, star hair wither, mound dish, the wind-weed, radix bupleuri, plant mean height 15-25cm, always degree of covering 50-80%.
6, edaphon, the distribution of microorganism and liveness situation are in the prairie Tricholomataceae soil for growth, the quantity of aerophile bacterium, fungi and liveness and prairie Tricholomataceae fruit body have a positive correlation, the prairie Tricholomataceae fruit body emergence period also is that aerophile bacterium, fungi monoid quantity are more than active period; Spore bacteria, actinomycetic quantity and liveness and prairie Tricholomataceae mycelial growth have positive correlation, and the prairie Tricholomataceae mycelia emergence period also is that spore bacteria, actinomycetes monoid quantity are more than active period; Draw thus spore bacteria, actinomycetes are conducive to the prairie Tricholomataceae mycelial growth, aerophile bacterium, fungi are conducive to the prairie Tricholomataceae fruit body and occur, and each monoid quantity of edaphon and mushroom mycelium interaction provide special environmental condition for the formation of fruit body development and mushroom.
7, the chemical-biological activities of soil, by the research to the soil biochemistry activity, can further understand the metabolic process of prairie Tricholomataceae soil for growth, the nutrition such as the biochemistry intensity of regulating the white mushroom soil for growth of control and carbon in soil, nitrogen, phosphorus are had important effect.Soil Respiration is the highest when the white mycelial growth in grassland, has positive correlation, illustrate that mycelia has vigorous respiratory metabolism when growth, the activity of invertase, phosphatase, urase is lower prairie Tricholomataceae mycelia and sporophore growth, do not have positive correlation, show that mycelial growth has inhibitory action to its activity.
8, the carbon nitrogen source nutritive peculiarity of prairie Tricholomataceae, prairie Tricholomataceae mycelia and fruit body optimum carbon source are sucrose, soluble starch, maltose, glucose etc., the amount of carbon source is very large for mycelia and fruit body impact, primary carbon source is not enough can to affect mycelial growth, prolong vegetative period, reduce the biomass of fruit body, affect output; Prairie Tricholomataceae mycelia and fruit body optimum nitrogen source are beef extract, yeast extract, peptone, ammonium nitrate etc., nitrogenous source deficiency can the exert an influence growth of mycelia and the growth of fruit body, and better C/N ratio is 20:1-40:1.
In sum, the genuineness of prairie Tricholomataceae is: the wild mycelia of prairie Tricholomataceae can survive at-45 ℃, begin to recover growth more than 5 ℃, the optimum growth temperature is 22-28 ℃, and the growth area is height above sea level 600-1500m grassland, Qiu Ling, year rainfall 400-600mm, 50% concentrates on the 7-8 month, rain hot same period, temperature difference per day 14-17 ℃, day and night temperature is large, year relative moisture is about 65%, the 7-8 month reaches more than 75%, and optimum carbon source is sucrose, soluble starch, optimum nitrogen source is yeast extract, beef extract, peptone, better C/N ratio is 20:1-40:1, and pH value is 6.0-7.0, V C, V B6And α-naphthyl acid can promote the growth of mycelia preferably, and vegetation structure is rhizome grass and tussock, and constructive species is sheep's hay.
Draw thus, wild prairie Tricholomataceae is under the comparatively stable prerequisite of temperature condition, and the principal element that affects the prairie Tricholomataceae growth is quantity of useful microbe and liveness in the carbon-nitrogen ratio of rainfall, soil and the soil.
Because the mutual comprehensive function of the genotypic and environment condition of prairie Tricholomataceae genuineness, formed the biological nature of prairie Tricholomataceae mycelia and fruit body, what existing prairie Tricholomataceae artificial domesticating cultivation method adopted mostly is the fungus growing technique that biological nature is not outstanding, adaptability is wider, can not satisfy the prairie Tricholomataceae required environmental condition of growing, exist under the artificial cultivation condition, though mycelia easily survives, but fruit body is difficult for the formation problem, and this is the main cause that do not make a breakthrough so far of artificial cultivation prairie Tricholomataceae technology just.
Summary of the invention
The invention provides a kind of field planting method, make the prairie Tricholomataceae lowered in field environment carry out artificial cultivation, realize the purpose of wild prairie Tricholomataceae large-scale production.
The technical scheme of prairie Tricholomataceae field planting method of the present invention is, adopt the female kind of artificial culture, the female kind of rejuvenation, cultivate original seed and cultivation strain, biological nature preparation planting material according to prairie Tricholomataceae, and make the in-built planting material of cultivation bag with the piece of sack, cultivation strain is accessed, be placed under the frame in the selected cultivation planning region, follow the genuineness of prairie Tricholomataceae, utilize local climate environment and natural vegetation condition in prairie Tricholomataceae ecosystem zone, indirect labor's intervening measure large-scale production prairie Tricholomataceae.
1, cultivates bacterial classification
Gather and select healthy and strong prairie Tricholomataceae to make female the kind, under aseptic condition, cut fresh wild prairie Tricholomataceae fruit body, choose the meat bacteria organization of cap stem boundary sheet, immigration contains in the vessel of PDA medium, put it in the insulating box and cultivate, cover with culture vessel when mycelia and namely finish female making of planting; The mother who obtains is planted containing in the PDA culture vessel of implantation expansion, putting into insulating box cultivates, mycelia is covered with culture vessel and namely finishes female Culturing of planting, the female kind of rejuvenation mycelia is put into-20 ℃ of insulating boxs together with the vessel that contain the PDA medium preserve, treat Second Year cultivation cultivation original seed and cultivation strain.
The May of Second Year the female kind of rejuvenation mycelia progressively being heated up reaches about 10 ℃, puts into the PDA culture vessel of expansion after mycelia recovers growth, cultivates in insulating box, obtains the prairie Tricholomataceae original seed after the full culture vessel of mycelial growth; The prairie Tricholomataceae original seed that obtains is put into the medium container of expansion, in 20-25 ℃ environment, naturally cultivate, namely make in batches prairie Tricholomataceae cultivation strain when mycelium covers with medium container.
Described female PDA medium component of planting is potato 220g/L, sucrose 100g/L, dipotassium hydrogen phosphate 5g/L, V B620mg/L, yeast extract 5g/L, agar 50g/L, water 100ml, ratio preparation, PH 6.0-7.0;
The female PDA medium component of planting of described rejuvenation is sucrose 50g/L, maltose 50g/L, peptone 10g/L, dipotassium hydrogen phosphate 5g/L , Hai acid magnesium 1g/L, V B620mg/L, water 100ml, ratio preparation, PH 6.0-7.0;
The PDA medium of described original seed is identical with the female PDA medium of planting of rejuvenation;
The PDA medium component of described cultivation strain is oat 30%-50%, rotten grass meal 30-35%, cow dung 20-30%, land plaster 1-5%, yeast extract 5-10%, sucrose 2-5%, V B61-5%, material-water ratio example 1:0.8, the ratio preparation, PH is 6.0-7.0.
2, planting material is made, the prescription of planting material is cow dung 40%, rotten grass bits 25%, chestnut soil 25%, land plaster 2%, superphosphate 2%, sucrose 2%, yeast extract 4%, planting material is taked composting process processing, cow dung, rotten grass meal, chestnut soil are successively stacked, successively add land plaster, superphosphate, sucrose, yeast extract, water is drenched, turning when material temperature reaches 70-80 ℃ turns evenly batching through 4-5 turning, and ferments, C/N ratio reaches 35:1, moisture keeps 60-65%, and PH is adjusted at 6.0-7.0, and is for subsequent use after the sterilization.
3, select the original ecology cultivating environment, ecosystem refers to ecotope and the condition that the prairie Tricholomataceae self-sow is bred, ecosystem environment of the present invention be select prairie Tricholomataceae ecosystem vegetatively, take full advantage of physioclimate, natural vegetation, soil types, edaphon, soil organismsization are active, prairie Tricholomataceae is satisfied in carbon nitrogen source nutrition biological nature and genuineness, the employing manual intervention householder method that natural environment and condition can not satisfy or supply with is adjusted.In prairie Tricholomataceae ecosystem zone, consider the selected grassland of the basic conditions such as water resource, traffic and carry out the scale cultivation, employing fence mode is protected and is managed.
4, indirect labor's Facilities Construction, in the cultivation area, build the facilities such as micro spray irrigation facility, pergola facility, climatic environment monitoring, realize climate monitoring, automatic micro-sprinkling irrigation is irrigate and overlay film humidification, overlay film shading, forms the ability of man-made irrigation, humidification, adjustment temperature, regulating illumination.
5, cultivation management method, adopt the piece of sack to be processed into cultivation bag, the planting material of in-built composting, in the planting material in the prairie Tricholomataceae cultivation strain access bag, cultivation bag is put under the frame in the planning construction cultural area, on cover rotten grass or rotten grass bits, or the grassland under the frame of cultivation area digs shallow ridges, covers rotten grass or rotten grass bits on placing the cultivation bag of access cultivation strain in the ditch.Note observing weather and liquid manure situation in management, when natural environment can not satisfy the growth conditions of prairie Tricholomataceae, utilize micro spray irrigation to moisturize, increase humidity, utilize the pergola facility overlay film to improve temperature or sunshade, all right spray nutritious liquor is fertile, adjusts pH value, guarantees ecosystem environment and the condition of prairie Tricholomataceae.
Further, because adopt climate monitoring, automatic micro-sprinkling irrigation is irrigate and the facility such as overlay film, environment and the condition of field cultivating prairie Tricholomataceae have been improved, make the one and a half months of the vegetative period of prairie Tricholomataceae fruit body by the early August under nature (fruit body emergence period) to late September (the mycelia decline phase), before prolong to early July, after prolong by three months of late September, improved output, increased cultivation benefit.
The invention has the beneficial effects as follows, adopt the female kind of artificial culture rejuvenation, cultivate original seed and cultivated species, biological nature preparation planting material according to prairie Tricholomataceae, utilize physioclimate environment and vegetation condition in the former growth attitude of prairie Tricholomataceae territory, indirect labor's intervening measure, the large-scale production prairie Tricholomataceae, broken through the difficult point of prairie Tricholomataceae artificial cultivation aspect biological nature, genuineness, so that endangered prairie Tricholomataceae has been realized artificial cultivation, and kept the wild white mushroom nutritious, delicious aromatic edibility and medicinal, health care value.This cultivation method is not used fertilizer and pesticide; do not destroy grassland ecology; kept natural, pollution-free, the green wild characteristic of cultivating of prairie Tricholomataceae; prolonged the growth emergence period of fruit body; improved the capacity and output of prairie Tricholomataceae, and this cultivation method is applicable to batch production production and commerial growing, this cultivation method economizes on resources; increase the grassland nutrient, protected ecology of grassland.
Embodiment
Below in conjunction with embodiment and embodiment prairie Tricholomataceae original ecology cultivating method of the present invention is further specified.
Embodiment 1
Cultivating bacterial classification selects healthy and strong wild prairie Tricholomataceae to make female the kind, under aseptic condition, cut fresh wild prairie Tricholomataceae fruit body, choose the meat bacteria organization at cap stem circle place, immigration is equipped with and is put into insulating box in the vessel of PDA medium and cultivate, temperature remains on 25-28 ℃, through 25-30 days cultivation, mycelia was covered with culture vessel and namely finishes female making of planting; The mother who obtains is planted being equipped with in the PDA culture medium vessel of implantation expansion put into insulating box, temperature remains on 25-28 ℃, cultivation through 25-30 days, mycelia is covered with culture vessel and namely finishes female Culturing of planting, the female kind of rejuvenation mycelia is put into-20 ℃ of insulating boxs preserve, original seed to be cultivated and cultivated species use.
When cultivating original seed and cultivated species, plant mycelia and progressively heat up and reach about 10 ℃ rejuvenation is female, put into the PDA culture medium vessel of expansion behind the female kind of the rejuvenation mycelia recovery growing ability, under 25-28 ℃ of constant temperature, cultivated 20-25 days, after mycelia is covered with culture vessel, namely obtain the prairie Tricholomataceae original seed; The prairie Tricholomataceae original seed is put into the medium container of expansion, place 20-25 ℃ the interior naturally cultivation of environment, through 20-25 days self-sows, namely obtain in batches prairie Tricholomataceae cultivation strain when mycelium covers with culture vessel.
The described PDA medium component of cultivating female kind is potato 220g/L, sucrose 100g/L, dipotassium hydrogen phosphate 5g/L, V B620mg/L, yeast extract 5g/L, agar 50g/L, water 100ml, ratio preparation, PH 6.0-7.0.
The female PDA medium component of planting of described rejuvenation is sucrose 50g/L, maltose 50g/L, peptone 10g/L, dipotassium hydrogen phosphate 5g/L , Hai acid magnesium 1g/L, V B620mg/L, water 100ml, ratio preparation, PH 6.0-7.0.
The PDA medium component of described mycelia original seed is identical with the PDA medium component of the female kind of rejuvenation mycelia.
The PDA medium component of described cultivated species is oat 30%-50%, rotten grass bits 30-35%, cow dung 20-30%, land plaster 1-5%, yeast extract 5-10%, sucrose 2-5%, V B61-5%, material-water ratio are 1:0.8, PH 6.0-7.0, and described rotten grass bits are rhizome grass and the tussock of prairie Tricholomataceae natural vegetation.
Embodiment 2
Planting material is made, and the prescription of planting material is cow dung 40%, rotten grass bits 25%, chestnut soil 25%, land plaster 2%, superphosphate 2%, sucrose 2%, yeast extract 4%.Planting material is taked composting process processing, cow dung, rotten grass bits, chestnut soil are successively stacked, successively added land plaster, superphosphate, water is drenched, turning when material temperature reaches 70-80 ℃, make to prepare burden through 4-5 turning and mix thoroughly, and ferment, put into the last time sucrose and yeast extract during turning, carbon nitrogen reaches 35:1, moisture keeps 60-65%, and PH is adjusted at 6.0-7.0, and is for subsequent use after the sterilization.
Embodiment 3
Select the original ecology cultivating environment, select vegetatively the cultivation area in the prairie Tricholomataceae ecosystem, consider the definite cultivation of the conditions such as water resource, traffic grassland and carry out planting scale, employing fence mode is protected and is managed.
Embodiment 4
Indirect labor's Facilities Construction, in the cultivation planning region, build the facilities such as micro spray irrigation facility, pergola facility, climatic environment monitoring, realization climate monitoring automatic micro-sprinkling irrigation is irrigate and the overlay film humidification, the overlay film shading forms the ability that grassland wild white mushroom is cultivated man-made irrigation, humidification, adjustment temperature, regulating illumination on a large scale.
Embodiment 5
Cultivation management method, adopt the piece of sack to be processed into the cultivation bag of long 50cm, wide 30cm, thick 20cm, the planting material of in-built composting, in the planting material in the prairie Tricholomataceae cultivation strain access bag, cultivation bag is pressed horizontal interval 60cm, vertical interval 20cm in a row puts under the frame of cultivation planning region, on cover the rotten grass of 3-5cm or rotten grass bits, or cultivating the shallow ridges that digs dark 25cm, wide 35cm under the planning region frame, shallow ridges interval 60cm covers rotten grass or the rotten grass bits of 3-5cm on placing postvaccinal cultivation bag in the ditch.Note observing weather and liquid manure situation in management, when natural environment can not satisfy the biological growth conditions of prairie Tricholomataceae, utilize micro spray irrigation, moisturize, increase humidity, utilize the plastic greenhouse facility to improve temperature or regulating illumination, can also spray nutrition fertilizer, adjust pH value, the ecosystem growing environment and the condition that keep prairie Tricholomataceae realize the extensive field cultivating of prairie Tricholomataceae and production.

Claims (6)

1. prairie Tricholomataceae field planting method, adopt the female kind of artificial culture, the female kind of rejuvenation, cultivate original seed and cultivated species, biological nature according to prairie Tricholomataceae is prepared planting material, and is processed into the in-built planting material of cultivation bag with the piece of sack, and cultivation strain is accessed, be placed under the frame in the selected cultivation planning region, follow the genuineness of prairie Tricholomataceae, utilize local climate and natural vegetation condition in prairie Tricholomataceae ecosystem zone, indirect labor's intervening measure large-scale production prairie Tricholomataceae.
2. a kind of prairie Tricholomataceae field planting method according to claim 1 is characterized in that, adopts the female kind of artificial culture, the female kind of rejuvenation, and the method for cultivating original seed and cultivated species is:
Under aseptic condition, cut fresh wild prairie Tricholomataceae fruit body, choose the meat bacteria organization of cap stem intersection, immigration is equipped with and is put into insulating box in the vessel of PDA medium and cultivate, temperature remains on 25-28 ℃, through 25-30 days cultivation, mycelia was covered with culture vessel and namely finishes female making of planting;
The mother who obtains is planted being equipped with in the PDA culture medium vessel of implantation expansion put into insulating box, temperature remains on 25-28 ℃, through 25-30 days cultivation, mycelia was covered with culture vessel and namely finishes female Culturing of planting, and the female kind of rejuvenation mycelia is put into-20 ℃ of insulating boxs preserve;
When cultivating original seed and cultivated species, plant mycelia and progressively heat up and reach about 10 ℃ rejuvenation is female, plant the PDA culture vessel of putting into expansion after mycelia recovers growing ability when rejuvenation is female, under 25-28 ℃ of constant temperature, cultivated 20-25 days, after mycelia is covered with culture vessel, namely obtain the prairie Tricholomataceae original seed;
The prairie Tricholomataceae original seed is put into the medium container of expansion, place in 20-25 ℃ the environment and naturally cultivate, through 20-25 days self-sows, cover with medium container when mycelia and namely obtain in batches prairie Tricholomataceae cultivation strain.
3. a kind of prairie Tricholomataceae field planting method according to claim 1 is characterized in that, cultivating female PDA medium component of planting is potato 220g/L, sucrose 100g/L, dipotassium hydrogen phosphate 5g/L, V B620mg/L, yeast extract 5g/L, agar 50g/L, water 100ml, ratio preparation, PH 6.0-7.0;
The female PDA medium component of planting of rejuvenation is sucrose 50g/L, maltose 50g/L, peptone 10g/L, dipotassium hydrogen phosphate 5g/L , Hai acid magnesium 1g/L, V B620mg/L, water 100ml, ratio preparation, PH 6.0-7.0;
The PDA medium component of cultivating original seed is identical with the PDA medium component of female kind rejuvenation;
The PDA medium component of cultivating cultivated species is oat 30%-50%, rotten grass bits 30-35%, cow dung 20-30%, land plaster 1-5%, yeast extract 5-10%, sucrose 2-5%, V B61-5%, material-water ratio are 1:0.8, and PH is 6.0-7.0, and described rotten grass bits are rhizome grass and the tussock of prairie Tricholomataceae natural vegetation.
4. a kind of prairie Tricholomataceae field planting method according to claim 1 is characterized in that, the prescription of planting material is cow dung 40%, rotten grass bits 25%, chestnut soil 25%, land plaster 2%, superphosphate 2%, sucrose 2%, yeast extract 4%; Planting material is taked composting process processing, cow dung, rotten grass bits, chestnut soil are successively stacked, successively added land plaster, superphosphate, water is drenched, turning when material temperature reaches 70-80 ℃, make to prepare burden through 4-5 turning and mix thoroughly, and ferment, put into the last time sucrose and yeast extract during turning, carbon nitrogen reaches 35:1, moisture keeps 60-65%, and PH is adjusted at 6.0-7.0, rear sterilization.
5. a kind of prairie Tricholomataceae field planting method according to claim 1 is characterized in that, indirect labor's facility is to build micro spray irrigation facility, pergola facility, climatic environment monitoring facilities in the cultivation planning region.
6. a kind of prairie Tricholomataceae field planting method according to claim 1, it is characterized in that, adopt the piece of sack to be processed into the cultivation material bag of long 50cm, wide 30cm, thick 20cm, the planting material of in-built composting, cultivation strain is accessed in the planting material, cultivation bag is pressed horizontal interval 60cm, vertical interval 20cm in a row is placed under the frame of cultivation planning region, on cover the rotten grass of 3-5cm or rotten grass bits, or cultivating the shallow ridges that digs dark 25cm, wide 30cm under the planning region frame, shallow ridges interval 60cm covers rotten grass or the rotten grass bits of 3-5cm on placing postvaccinal cultivation material bag in the ditch.
CN201210374298.3A 2012-10-03 2012-10-03 Field cultivation method for grassland white mushrooms Expired - Fee Related CN102860218B (en)

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CN106613172A (en) * 2016-11-28 2017-05-10 广州甘润堂生物科技有限公司 Method for original ecological planting of genuine shorthairy antenoron in Guangdong Conghua Heavenly lake
CN107964513A (en) * 2017-12-25 2018-04-27 内蒙古自治区农牧业科学院 Tricholoma mongolicum new strains prairie Tricholomataceae 1 and its selection
CN108048335A (en) * 2017-12-25 2018-05-18 内蒙古自治区农牧业科学院 Tricholoma mongolicum new strains prairie Tricholomataceae 2 and its selection
CN108235900A (en) * 2017-12-25 2018-07-03 内蒙古自治区农牧业科学院 A kind of Tricholoma mongolicum new strains prairie Tricholomataceae 3 and its acclimation method
CN108048335B (en) * 2017-12-25 2021-02-05 内蒙古自治区农牧业科学院 Noval strain grassland white mushroom No. 2 of Mongolian tricholoma mongolicum and breeding method thereof
CN107964513B (en) * 2017-12-25 2021-03-23 内蒙古自治区农牧业科学院 Noval strain grassland white mushroom No.1 of Mongolian tricholoma mongolicum and breeding method thereof
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