CN102845415A - Plant respiration inhibitor for tree transplanting and preparation method of plant respiration inhibitor - Google Patents
Plant respiration inhibitor for tree transplanting and preparation method of plant respiration inhibitor Download PDFInfo
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- CN102845415A CN102845415A CN2012102940686A CN201210294068A CN102845415A CN 102845415 A CN102845415 A CN 102845415A CN 2012102940686 A CN2012102940686 A CN 2012102940686A CN 201210294068 A CN201210294068 A CN 201210294068A CN 102845415 A CN102845415 A CN 102845415A
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Abstract
The invention relates to the field of plant conditioning agents, and discloses a plant respiration inhibitor for tree transplanting and a preparation method of the plant respiration inhibitor. The plant respiration inhibitor is prepared by mixing TritonX-100, hydroxylamine hydrochloride, acetone, chloroacetic acid solution and hydroquinone according to a certain proportion. With the adoption of the plant respiration inhibitor, a respiration function of the plant is adjusted, particularly, the quantity of absorbing roots of the trees is reduced before and after the tree transplanting, nutrient substances absorbed in the soil are reduced, and a respiration function of the trees still works, and nutrient substances consumed by the trees is greater than the absorbed nutrient substances so as to result in reduction of survival ratios of the transplanted trees.
Description
Technical field
The present invention relates to the plant modifying agent field, relate in particular to a kind of plant respiration inhibitor for tree grafting growth and preparation method thereof.
Background technology
Tree grafting growth is one of basis of afforestation, green barren hill engineering, for guaranteeing surviving of transplanting, play the pick pre-treatment to transplanting in the rear maintenance processes trees, it mainly is the physiological metabolism balance that guarantees transplanting, this process requires all very high in technology and expense, the people that need to have level professional technology carries out this work, is subject to certain restrictions universal.Therefore, by the Respiratory control to trees, reduce that tree is recuperated minute, water consumption, and then regulate the transplanting physiological metabolism, make transplanting adapt to as early as possible new growing environment, the survival rate of raising tree grafting growth seems particularly important.
The regulating plant respiration is one of key link of regulation and control trees physiological metabolism balance.Be in the trees before and after transplanting, because the change in habitat, need to reduce the tree vigo(u)r metabolism, reduce the consumption of nutrient and moisture, to adapt to new growing environment, the respiration of trees has dual-use function, on the one hand decomposition tree is recuperated minute, to satisfy normally carrying out of tree body vital movement, on the other hand, by the synthetic needed enzyme of organic nutrient substance in the respiration generation tree body.Respiratory metabolism is too fast, will consume too much the nutrient that stores in the tree body, and be in the tree body of transplanting stage, and the function that absorbs nutriment from the external world significantly reduces, and the ability of self synthetic nutriment also descends to some extent, therefore, in the tree grafting growth process, the tree body is sprayed an amount of respiration inhibitor, reduce the respiratory rate of transplanting, guarantee the tree vigo(u)r metabolic balance, can obviously improve the survival rate of transplanting.
Current plant respiration inhibitor Main Function is the photorespiration of plant is regulated and control, thereby improves the Net Photosynthetic Rate of plant.But this type of respiration inhibitor needs strict concentration control, and concentration control is unreasonable, can cause high light intensity to the destruction of photosynthetic organs, reduces the photosynthesis of plant.In addition, this type of respiration inhibitor is multiplex in crop class herbaceous plant, take the output that improves crop as purpose.More with sodium hydrogensulfite (NaHSO as using now
4) be the light respiring inhibitor of main component, the photorespiration of crop is suppressed to have good action effect, and to the inhibitory action DeGrains of trees.With NaN
3For the respiration inhibitor of main component has obvious effect to the respiration inhibition effect of plant seed, undesirable to the respiration inhibition effect of plant leaf blade.
The present invention is by the amount of energy charge in the regulating plant Respiratory Metabolism Pathway and the amount of ethene, reduces respiratory rate, reduces the consumption of photosynthetic product, slows down tree metabolism activity speed.The present invention can be used for draft, woody plant.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of plant respiration inhibitor for tree grafting growth and preparation method thereof is provided.
In order to solve the problems of the technologies described above, the present invention is solved by following technical proposals.
Be used for the plant respiration inhibitor of tree grafting growth, comprise carboxymethyl azanol half hydrochloride and Triton X-100, the weight ratio of carboxymethyl azanol half hydrochloride and Triton X-100 is 1:100 ~ 3:100.
Be used for the plant respiration inhibitor of tree grafting growth, every 2500ml plant respiration inhibitor is mainly made by the raw material of following content:
Tris (tri methylol amino methane) 6.05g,
NaCl 8.75g，
Triton X-100 (Triton X-100) 7.74ml,
NH
2OHHCl (hydroxylamine hydrochloride) 129-149g,
Acetone 120-138ml,
Frozen water 98-117g,
Chloroacetic acid solution 103-132g,
Quinhydrones 0.001-0.01g,
37% hydrochloric acid 95-105ml,
NaOH solution is an amount of,
9.5-10.5% hydrochloric acid is an amount of,
Distilled water is an amount of.
Concentration of the present invention is mass concentration.
As preferably, the concentration of chloroacetic acid solution is 1.4mol/L.
As preferably, the NaOH solution concentration is 40%-50%.
Be used for the preparation method of the plant respiration inhibitor of tree grafting growth, may further comprise the steps:
The first step takes by weighing raw material;
Second step mixes Tris and NaCl, adds distilled water to 500ml, drips 9.5-10.5% salt acid for adjusting pH value to 7.4 while stir, and adds distilled water to 1000ml again; Then add Triton X-100, again add 1000ml distilled water, in 37 ℃～40 ℃ water-baths, heat 2～3h, Triton X-100 is fully dissolved, for subsequent use;
The 3rd step was dissolved in 200ml distilled water with hydroxylamine hydrochloride, added acetone, was heated to 40-50 ℃, and stirring reaction 30min drips NaOH solution, regulates PH to 8.5-9, is cooled to room temperature, places 12h, filters, and drying gets acetoxime;
The 4th step, acetoxime and frozen water are mixed, then measure the NaOH solution of 121-123ml, slowly add and be stirred to acetoxime and dissolve fully, again to wherein slowly adding chloroacetic acid solution, be warming up to 80 ℃, reaction 3h after the cooling, slowly uses the 9.5-10.5% hcl acidifying to pH0.6-1.0, filter, get acetone carboxymethyl oxime solution;
The 5th step added reactor with acetone carboxymethyl oxime solution and quinhydrones, added 100ml distilled water again, stirred after the mixing, dripped 37% hydrochloric acid 100ml, added rear back flow reaction 1 hour, got carboxymethyl azanol half hydrochloride;
In the 6th step, the Triton X-100 that second step is for subsequent use adds carboxymethyl azanol half hydrochloride that the 5th step made, and mixes, and can get the plant respiration inhibitor.
As preferably, the concentration of plant respiration inhibitor is 1.168% ~ 1.313%.
Hydroxylamine-O-acetic acid Hemihydrochloride has another name called O-(carboxymethyl) azanol half hydrochloride, Aminoxyacetic Hemihydrochloride; Hydroxylamine-O-acetic acid Hemihydrochloride, Hydroxylamine-O-acetic acid Hemihydrochloride, O-(carboxymethyl) azanol half hydrochloride is the hapten as the preparation ketone compounds, and preparation chemiluminescent material N (4-aminobutyl) the different luminol of N-ethyl (ABEI) is with the mark ketone.
The present invention suppresses the characteristic of glutamic-pyruvic transaminase, acts on plant corpus and regulates the quantity that aspartic acid is converted into methionine, and synthesizing of regulating plant endogenous hormones ethene suppresses respiratory rate.
The respiration that the present invention is directed to plant is regulated, especially before and after transplanting being transplanted, trees absorb the radical amount and reduce, the nutriment that absorbs from soil reduces, and the trees respiration is still being carried out, the nutriment that trees consume causes the transplanting survival rate to reduce more than the nutriment that absorbs.
Embodiment
Below in conjunction with subordinate list and embodiment the present invention is described in further detail.
Embodiment 1
A kind of plant respiration inhibitor for tree grafting growth, made by the raw material that comprises following content:
Tris (tri methylol amino methane) 6.05g,
NaCl 8.75g，
Triton X-100 (Triton X-100) 7.74ml,
NH
2The OHHCl(hydroxylamine hydrochloride) 129g,
Acetone 120ml,
Frozen water 98g,
Chloroacetic acid solution 103g,
Quinhydrones 0.001g,
37% hydrochloric acid 95ml,
NaOH solution is an amount of,
9.5-10.5% hydrochloric acid is an amount of,
Distilled water is an amount of.
Wherein, the concentration of chloroacetic acid solution is 1.4mol/L, and the NaOH solution concentration is 40%.
Its preparation method may further comprise the steps:
The first step takes by weighing raw material by said ratio;
Second step mixes Tris and NaCl, adds distilled water to 500ml, drips 9.5-10.5% salt acid for adjusting pH value to 7.4 while stir, and adds distilled water to 1000ml again; Then add Triton X-100, again add 1000ml distilled water, in 37 ℃ of water-baths, heat 2h, Triton X-100 is fully dissolved, for subsequent use;
The 3rd step was dissolved in 200ml distilled water with hydroxylamine hydrochloride, added acetone, was heated to 40 ℃, and stirring reaction 30min drips NaOH solution, regulates PH to 8.5, is cooled to room temperature, places 12h, filters, and drying gets acetoxime;
The 4th step, acetoxime and frozen water are mixed, then measure the NaOH solution of 121ml, slowly add and be stirred to acetoxime and dissolve fully, again to wherein slowly adding chloroacetic acid solution, be warming up to 80 ℃, reaction 3h after the cooling, slowly uses the 9.5-10.5% hcl acidifying to pH0.6-1.0, filter, get acetone carboxymethyl oxime solution;
The 5th step added reactor with acetone carboxymethyl oxime solution and quinhydrones, added 100ml distilled water again, stirred after the mixing, dripped 37% hydrochloric acid 100ml, added rear back flow reaction 1 hour, got carboxymethyl azanol half hydrochloride;
In the 6th step, the Triton X-100 that second step is for subsequent use adds carboxymethyl azanol half hydrochloride that the 5th step made, and mixes, and can get concentration and be 1.168% plant respiration inhibitor.
Embodiment 2
A kind of plant respiration inhibitor for tree grafting growth, made by the raw material that comprises following content:
Tris (tri methylol amino methane) 6.05g,
NaCl 8.75g，
Triton X-100 (Triton X-100) 7.74ml,
NH
2The OHHCl(hydroxylamine hydrochloride) 149g,
Acetone 138ml,
Frozen water 117g,
Chloroacetic acid solution 132g,
Quinhydrones 0.01g,
37% hydrochloric acid 105ml,
NaOH solution is an amount of,
9.5-10.5% hydrochloric acid is an amount of,
Distilled water is an amount of.
Wherein, the concentration of chloroacetic acid solution is 1.4mol/L, and the NaOH solution concentration is 50%.
Its preparation method may further comprise the steps:
The first step takes by weighing raw material by said ratio;
Second step mixes Tris and NaCl, adds distilled water to 500ml, drips 9.5-10.5% salt acid for adjusting pH value to 7.4 while stir, and adds distilled water to 1000ml again; Then add Triton X-100, again add 1000ml distilled water, in 40 ℃ of water-baths, heat 3h, Triton X-100 is fully dissolved, for subsequent use;
The 3rd step was dissolved in 200ml distilled water with hydroxylamine hydrochloride, added acetone, was heated to 50 ℃, and stirring reaction 30min drips NaOH solution, regulates PH to 9, is cooled to room temperature, places 12h, filters, and drying gets acetoxime;
The 4th step, acetoxime and frozen water are mixed, then measure the NaOH solution of 123ml, slowly add and be stirred to acetoxime and dissolve fully, again to wherein slowly adding chloroacetic acid solution, be warming up to 80 ℃, the reaction 3h, after the cooling, slowly with the 9.5-10.5% hcl acidifying to pH be 0.6-1.0, filter, get acetone carboxymethyl oxime solution;
The 5th step added reactor with acetone carboxymethyl oxime solution and quinhydrones, added 100ml distilled water again, stirred after the mixing, dripped 37% hydrochloric acid 100ml, added rear back flow reaction 1 hour, got carboxymethyl azanol half hydrochloride;
In the 6th step, the Triton X-100 that second step is for subsequent use adds carboxymethyl azanol half hydrochloride that the 5th step made, and mixes, and can get concentration and be 1.313% plant respiration inhibitor.
Embodiment 3
A kind of plant respiration inhibitor for tree grafting growth, made by the raw material that comprises following content:
Tris (tri methylol amino methane) 6.05g,
NaCl 8.75g，
Triton X-100 (Triton X-100) 7.74ml,
NH
2The OHHCl(hydroxylamine hydrochloride) 138g,
Acetone 129ml,
Frozen water 110g,
Chloroacetic acid solution 122g,
Quinhydrones 0.005g,
37% hydrochloric acid 100ml,
NaOH solution is an amount of,
9.5-10.5% hydrochloric acid is an amount of,
Distilled water is an amount of.
Wherein, the concentration of chloroacetic acid solution is 1.4mol/L, and the NaOH solution concentration is 48%.
Its preparation method may further comprise the steps:
The first step takes by weighing raw material by said ratio;
Second step mixes Tris and NaCl, adds distilled water to 500ml, drips 9.5-10.5% salt acid for adjusting pH value to 7.4 while stir, and adds distilled water to 1000ml again; Then add Triton X-100, again add 1000ml distilled water, in 38 ℃ of water-baths, heat 2.5h, Triton X-100 is fully dissolved, for subsequent use;
The 3rd step was dissolved in 200ml distilled water with hydroxylamine hydrochloride, added acetone, was heated to 40-50 ℃, and stirring reaction 30min drips NaOH solution, regulates PH to 8.8, is cooled to room temperature, places 12h, filters, and drying gets acetoxime;
The 4th step, acetoxime and frozen water are mixed, then measure the NaOH solution of 122ml, slowly add and be stirred to acetoxime and dissolve fully, again to wherein slowly adding chloroacetic acid solution, be warming up to 80 ℃, the reaction 3h, after the cooling, slowly with the 9.5-10.5% hcl acidifying to pH be 0.6-1.0, filter, get acetone carboxymethyl oxime solution;
The 5th step added reactor with acetone carboxymethyl oxime solution and quinhydrones, added 100ml distilled water again, stirred after the mixing, dripped 37% hydrochloric acid 100ml, added rear back flow reaction 1 hour, got carboxymethyl azanol half hydrochloride;
In the 6th step, the Triton X-100 that second step is for subsequent use adds carboxymethyl azanol half hydrochloride that the 5th step made, and mixes, and can get concentration and be 1.246% plant respiration inhibitor.
Embodiment 4
Test material: 10 unified sowings, unified management, consistent 5 years fragrant camphor trees of life of specification, and be numbered 1-10.
5 leaves are chosen in middle level at every tree tree crown, and make marks.Utilize the plant respiration analyzer to measure the respiratory rate of 5 leaves of every tree.10 tree tree crowns are evenly sprayed the plant respiration inhibitor that embodiment 1 makes, be diluted to the respiratory rate of again measuring index blade after 1.168% 1 week, and calculating mean value.The result is as follows:
Conclusion: the respiratory rate that sprays behind the plant respiration inhibitor reduces by 20%.Spray in 5 years fragrant camphor trees of life, help to reduce the loss of tree body nutriment, guarantee tree body metabolic balance, increase the transplanting survival rate.
Embodiment 5
Test material: 10 unified sowings, unified management, consistent 5 years fragrant camphor trees of life of specification, and be numbered 1-10.
5 leaves are chosen in middle level at every tree tree crown, and make marks.Utilize the plant respiration analyzer to measure the respiratory rate of 5 leaves of every tree.10 tree tree crowns are evenly sprayed the plant respiration inhibitor that embodiment 2 makes, be diluted to the respiratory rate of again measuring index blade after 1.313%, one week, and calculating mean value.The result is as follows:
Conclusion: the respiratory rate that sprays behind the plant respiration inhibitor reduces by 35%.Spray in 3 years fragrant camphor trees of life, help to reduce the loss of tree body nutriment, guarantee tree body metabolic balance, increase the transplanting survival rate.
Embodiment 6
Test material: 10 unified sowings, unified management, consistent 5 years fragrant camphor trees of life of specification, and be numbered 1-10.
5 leaves are chosen in middle level at every tree tree crown, and make marks.Utilize the plant respiration analyzer to measure the respiratory rate of 5 leaves of every tree.10 tree tree crowns are evenly sprayed the plant respiration inhibitor that embodiment 3 makes, be diluted to the respiratory rate of again measuring index blade after 1.246% 1 week, and calculating mean value.The result is as follows:
Conclusion: the respiratory rate that sprays behind the plant respiration inhibitor obviously reduces.Spray in 3 years fragrant camphor trees of life, respiratory rate reduces by 22%, reduces nutrient consumption.
Embodiment 7
Test material: 5 years fragrant camphor trees of life, 3 years living glossy privets, 3 years living red bayberries.The concentration that makes by embodiment 1,2,3 is diluted to respectively 1.168%, 1.246%, 1.313% plant respiration inhibitor, and 10 trees are chosen in each measurement at random, and every tree is chosen 5 leaves in the middle level of tree crown, and makes marks.Utilize the plant respiration analyzer to measure respiratory rate, spray 1.168%, 1.246%, 1.313% tree crown even breathing inhibitor, clear water with sprayer after, measure the blade that mark is crossed after the week.Comparative result is as follows after measuring:
3 years fragrant camphor trees of life
3 years living glossy privets
3 years living red bayberries
Conclusion: the respiratory rate that sprays behind the plant respiration inhibitor obviously reduces.Spray in Naturally growing plant species blade, branch, trunk, help to reduce respiratory rate, reduce nutrient consumption.Spray in transplanting, help to reduce the loss of tree body nutriment, guarantee tree body metabolic balance, increase the transplanting survival rate.
In a word, the above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to the covering scope of patent of the present invention.
Claims (6)
1. be used for the plant respiration inhibitor of tree grafting growth, comprise carboxymethyl azanol half hydrochloride and Triton X-100, the weight ratio of carboxymethyl azanol half hydrochloride and Triton X-100 is 1:100 ~ 3:100.
2. the plant respiration inhibitor for tree grafting growth according to claim 1, every 2500ml plant respiration inhibitor is mainly made by the raw material of following content:
Tris 6.05g，
NaCl 8.75g，
Triton X-100 7.74ml，
NH
2OH·HCl 129-149g，
Acetone 120-138ml,
Frozen water 98-117g,
Chloroacetic acid solution 103-132g,
Quinhydrones 0.001-0.01g,
37% hydrochloric acid 95-105ml,
NaOH solution is an amount of,
9.5-10.5% hydrochloric acid is an amount of,
Distilled water is an amount of.
3. the plant respiration inhibitor for tree grafting growth according to claim 1, it is characterized in that: the concentration of chloroacetic acid solution is 1.4mol/L.
4. the plant respiration inhibitor for tree grafting growth according to claim 1, it is characterized in that: the NaOH solution concentration is 40%-50%.
5. the preparation method of the plant respiration inhibitor for tree grafting growth according to claim 2 may further comprise the steps:
The first step takes by weighing raw material;
Second step mixes Tris and NaCl, adds distilled water to 500ml, drips 9.5-10.5% salt acid for adjusting pH value to 7.4 while stir, and adds distilled water to 1000ml again; Then add Triton X-100, again add 1000ml distilled water, in 37 ℃～40 ℃ water-baths, heat 2～3h, Triton X-100 is fully dissolved, for subsequent use;
The 3rd step was dissolved in 200ml distilled water with hydroxylamine hydrochloride, added acetone, was heated to 40 ~ 50 ℃, and stirring reaction 30min drips NaOH solution, regulates PH to 8.5 ~ 9, is cooled to room temperature, places 12h, filters, and drying gets acetoxime;
The 4th step, acetoxime and frozen water are mixed, then get the NaOH solution of 121 ~ 123ml, slowly add and be stirred to acetoxime and dissolve fully, again to wherein slowly adding chloroacetic acid solution, be warming up to 80 ℃, reaction 3h after the cooling, slowly uses the 9.5-10.5% hcl acidifying to pH0.6 ~ 1.0, filter, get acetone carboxymethyl oxime solution;
The 5th step added reactor with acetone carboxymethyl oxime solution and quinhydrones, added 100ml distilled water again, stirred after the mixing, dripped 37% hydrochloric acid, added rear back flow reaction 1 hour, got carboxymethyl azanol half hydrochloride;
In the 6th step, the Triton X-100 that second step is for subsequent use adds carboxymethyl azanol half hydrochloride that the 5th step made, and mixes, and makes described plant respiration inhibitor.
6. the preparation method of the plant respiration inhibitor for tree grafting growth according to claim 5, it is characterized in that: the concentration of plant respiration inhibitor is 1.168% ~ 1.313%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111972491A (en) * | 2020-08-18 | 2020-11-24 | 贵州石阡佛顶山野生油茶油业有限公司 | Method for slowing down storage consumption of oil tea fruit oil |
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| US4735968A (en) * | 1985-06-05 | 1988-04-05 | Tulane Educational Fund | Method of treating tinnitus with AOAA |
Non-Patent Citations (3)
| Title |
|---|
| 《Plant Growth Regulation》 19931231 A.E.Bichara 等 The effect of aminooxyacetic acid and cytokinin combinations on carnation flower longevity 第161-167页 , 第13期 * |
| A.E.BICHARA 等: "The effect of aminooxyacetic acid and cytokinin combinations on carnation flower longevity", 《PLANT GROWTH REGULATION》 * |
| 孔繁蕾 等: "植物生长调节剂羧甲基羟胺半盐酸盐的合成", 《农药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111972491A (en) * | 2020-08-18 | 2020-11-24 | 贵州石阡佛顶山野生油茶油业有限公司 | Method for slowing down storage consumption of oil tea fruit oil |
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Application publication date: 20130102 |