CN102835289A - Method for radically treating apple cankers by microorganism pythium oligadrum - Google Patents
Method for radically treating apple cankers by microorganism pythium oligadrum Download PDFInfo
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- CN102835289A CN102835289A CN2012103445991A CN201210344599A CN102835289A CN 102835289 A CN102835289 A CN 102835289A CN 2012103445991 A CN2012103445991 A CN 2012103445991A CN 201210344599 A CN201210344599 A CN 201210344599A CN 102835289 A CN102835289 A CN 102835289A
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- 241000131360 Pythium oligandrum Species 0.000 claims description 24
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
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- 239000004202 carbamide Substances 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 7
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- 239000002068 microbial inoculum Substances 0.000 claims description 7
- 239000012752 auxiliary agent Substances 0.000 claims description 5
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- 241000361919 Metaphire sieboldi Species 0.000 claims description 3
- 230000032823 cell division Effects 0.000 claims description 3
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 3
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- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
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- 125000001181 organosilyl group Chemical group [SiH3]* 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for radically treating apple cankers by microorganism pythium oligadrum; the method comprises the following steps: (1) first, cleaning a tree trunk and tree branches with apple cankers by clean water so as to remove germs and dust attached to the surfaces of the tree trunk and the tree branches; (2) preparing a biocontrol bacterium liquid: pouring a pythium oligadrum bactericide into a container filled with a small amount of clean water; dipping and appropriately stirring the pythium oligadrum bactericide; pouring out supernatant after about 30 minutes; continuing to add clean water to dilute the supernatant; after the supernatant is diluted, adding an appropriate amount of plant nutrition regulators, white sugars, ureas and organic silicon additives to the diluted supernatant; sufficiently stirring the supernatant so as to obtain the uniform biocontrol bacterium liquid; and (3) after the surfaces of the tree trunk and the tree branches are dried, uniformly spraying or coating the biocontrol bacterium liquid on the tree trunk and the tree branches. Through directly spraying clean water on the tree trunk and the tree branches to clean the tree trunk and the tree branches and then spraying and coating the microorganism pythium oligadrum bactericide on the tree trunk and the tree branches to kill germs, the method for radically treating the apple cankers by the microorganism pythium oligadrum, provided by the invention, has the advantages of simplicity, convenience, easiness, enduring effect, no influence on tree vigor, safety and environment friendliness and is suitable for sustainable production of pollution-free, green and organic apples.
Description
Technical field
The present invention relates to the plant protection field, especially relate to a kind of microorganism pythium oligandrum that utilizes simple and easy to do, that effect is excellent and effect a radical cure the method for apple canker.
Background technology
Apple canker is commonly called as skin rot, and smelly skin is sick, is the important disease of northern China apple tree, and North China, northeast, the Northwest take place general.The bearing-age tree that the life in 6 years of mainly causing harm is above causes that tree vigo(u)r is weak, limb is withered, dead tree, even ruins the garden, sets general diseased plant rate 20%～30% greatly, and grave illness garden diseased plant rate reaches more than 80%, is the destructive disease that influences apple production.This disease cardinal symptom shows as the downright bad patch that the little protuberance of the water stain shape of large-scale bronzing on the doing of apple tree, branch bark, occurs, and quality is soft, and is easy to crack, often flows out yellowish-brown juice from sick portion, and the vinasse flavor is arranged.Later stage disease portion drying shrinkage, there is the crack at the edge, and sick skin surface grows pore, i.e. the germ fruit body.Branch, the mashed necrosis of dry rot when serious.If will keep the apple ordinary production, must have simple and easy to doly, effect is good, can continue the effective technology that prevention and control should disease.
Preventing and treating this sick conventional method is: in annual 10～December or 3～April of next year with sharp knife with apple tree major branch or trunk in spite of illness the skin of scar all wipe off, the degree of depth reaches xylem, and around good skin obliquely cut away 1～2 centimetre.Tangent plane requires smooth clean, is the inclined-plane of 45～60 degree, and sick scar is preferably scraped diamondwise or ellipse.Then the soup for preparing is spread upon with small brushes on the sick scar of scraping, the scope of smearing should surpass around the sick scar more than 2 centimetres, accomplishes to scrape one and is coated with one, and 2 Zhou Houzai smear once, and it is even, careful to smear the medicament requirement.Scrape the alignment request that takes stopgap measures: one wants thoroughly not only to want scraping variable color tissue, and will scrape off the good tissue about 0.5cm; Two is smooth, promptly spreads to fusiformis, do not stay the dead angle, do not turn the sharp turn, do not stay the hair stubble, in order to wound healing; Three want the repeatedly even coating in surface.The medicament of smearing has: 10 degree Baume lime sulfurs, 50～75 times of liquid of 60% sodium humate pulvis, 80 times of liquid of Fubiqing, S-921 zymotic fluid, 50 times of liquid of 70% thiophanate-methyl wetting powder+50 times of liquid of 50% thiram wettable powder add an amount of soya-bean oil or other plant oil, 40 times of liquid of 50% thiophanate-methyl wetting powder+50 times of liquid of 60% sodium humate add an amount of soya-bean oil or other plant is oily.
The limitation of conventional method:
1. traditional method of preventing and treating is to carry out rossing every year 10～December or 3～April of next year; Not that disease takes place by period; Just to then or the trunk of morbidity last year remove with the diseased tissues of branch, the germ of really infecting does not then carry out specific aim and prevents and treats;
2. the method for traditional treatment rot all selects to scrape bark, smears medicine then, and emphasis is to the scab that rots, and the rotten pathogenic bacteria of hiding in the healthy bark does not obtain handling, thereby rot can not get radical cure always;
3. traditional rossing is prevented and treated method and is taken very much manual work, and not only efficient is very low, eminence limb is awkward even can't implement, and exists control to omit, and whole structure is poor, can not realize radical cure;
4. the tree body can not normally be carried moisture and nutrient behind the rossing, scrapes skin portion and exposes throughout the year, attacked by extraneous bad weather; The trunk internal moisture is scattered and disappeared from sick portion easily, exposes to organize contaminated by multiple germ, and is rotten gradually; Whole tree vigo(u)r is badly damaged, and it is downright bad withered to cause complete stool eventually.
Summary of the invention
The technical problem that the present invention solved provide a kind of simple and easy to do, effect is excellent, the method for utilizing microorganism pythium oligandrum radical cure apple canker of no any pollution.
A kind of method of utilizing microorganism pythium oligandrum radical cure apple canker may further comprise the steps:
(1) at first with clear water cleaning morbidity trunk and branch, removes sticking supernatant germ and dust;
(2) preparation biocontrol microorganisms liquid: the pythium oligandrum microbial inoculum is poured in the container that fills a small amount of clear water; Soak and suitably stirring; Pour out supernatant after about 30 minutes; Continue to add clear water and dilute, add an amount of plant nutriment regulator, white sugar, urea and organosilicon auxiliary agent after the dilution again, stir and process biocontrol microorganisms liquid;
(3) treat biocontrol microorganisms liquid evenly to be sprayed after trunk and the branch dry tack free or be brushed on trunk and the branch.
The purpose of step in the said method (1) at first is cleaning morbidity trunk and branch, and its less important trunk and branch surface absorption portion moisture of making is beneficial to down pythium oligandrum spore germination and mycelial growth in the step, is convenient to catch germ.
The effect of step in the said method (2) interpolation plant nutriment regulator and white sugar, urea shows two aspects: the one, and for the pythium oligandrum bacterium provides carbon element and nitrogen nutrition, the growing of pythium oligandrum bacterium that captures germ (not obtaining source of nutrition) in particular for partly failing provides nutrition; The 2nd, stimulate the site of pathological change of trunk and branch to produce callus fast, thereby recover tree vigo(u)r.Add organosilyl effect and mainly be and help the pythium oligandrum spore, bring into play its parasitization to greatest extent attached to trunk, branch surface with along the bark surface and the internal extended infiltration.
Step in the said method (3) is sprayed or brushing biocontrol microorganisms liquid should or carry out at the cloudy day fine afternoon, and avoiding high light to be exposed to the sun influences the pythium oligandrum survival.
Method of the present invention, the every gram of wherein said pythium oligandrum microbial inoculum contains 10
6Spore adds said pythium oligandrum microbial inoculum 0.3 ~ 0.5 gram in every liter of biocontrol microorganisms liquid.
Method of the present invention, wherein said plant nutriment regulator are that plant Johnson & Johnson is plain or earthworm is fertile or the gibberellin or the basic element of cell division.
Method of the present invention, the addition of wherein said plant nutriment regulator are to add 1 ~ 4 milliliter or gram in per 1 liter of dilution.
Method of the present invention, the addition of wherein said white sugar and urea are to add 10 gram white sugar and 10 gram urea in every liter of biocontrol microorganisms liquid.
Method of the present invention, the addition of wherein said organosilicon auxiliary agent are 0.1% of dilution quality.
Method of the present invention begins control in the annual 5-8 month apple canker emergence period, is preferably in for the first time that disease takes place by early stage or the initial stage comes into effect, and uses once in according to circumstances 15 ~ 30 days later on.
The present invention need not strike off disease portion tissue, disease take place the critical period directly spray, brushing pythium oligandrum bacterial parasite liquid, directly the germ of parasitic disease portion and the hole skin through bark directly are deep into the inner constantly propagation of bark; Be close to, follow the tracks of germ; Germ is thoroughly killed, help pythium oligandrum successfully to set up parasitic relation as far as possible simultaneously, the performance effect of preventing and curing diseases better; Also stimulate the quick callus of trunk and branch, recover tree vigo(u)r rapidly.
The present invention adopts in the disease emergence period and directly sprays clear water and sprinkling, brushing biological and ecological methods to prevent plant disease, pests, and erosion microorganism pythium oligandrum bacterium liquid killing pathogenic bacteria; With strong points, method is simple, and effect is lasting; Do not influence tree vigo(u)r; Safety and environmental protection is fit to Sustainable Production nuisanceless, green, organic apple, and popularizing application prospect is wide.
Embodiment
For further specifying the present invention, specify in conjunction with following examples:
Embodiment 1:
Take place or the initial stage begins to carry out the chemical control first time early stage in annual 5-8 month canker of apple fruit, a situation arises uses once about 15 ~ 30 days based on disease.Select the cloudy day or use fine afternoon, with clear water spary trunk and big branch, treat that water does the back and evenly spray or the good pythium oligandrum biocontrol microorganisms suspension of brushing dilution on trunk and branch surface before using, avoid high light to be exposed to the sun influencing pythium oligandrum surviving.
The biocontrol microorganisms liquid and preparation method thereof:
With 10
6Spore/gram pythium oligandrum microbial inoculum 1.5-2.5 gram is poured into and is filled in the clean container that 1-3 rises clear water; Leave standstill the egg spore that made pythium oligandrum in 20-30 minute and from carrier, fully leach activation; Remove similar fine sand appearance carrier in the water, supernatant is diluted to 5 liters with clear water, in dilution, add 10 milliliters of plant Johnson & Johnson elements; Or 20 milliliters of earthworm fertilizer or the 5 gram gibberellin or the 5 gram basic elements of cell division; Add 50 gram white sugar, 50 gram urea again, (5 gram) the organosilicon auxiliary agent that adds 0.1% again stirs and gets final product.
In the annual 5-8 month, the field of apple canker morbidity just period, a situation arises carries out 3-5 time and prevent and treat dispenser according to disease.
Points for attention: pythium oligandrum biocontrol microorganisms dilution is now with the current, and sprinkling and brushing bacterium liquid are preferably in the cloudy day or carry out fine afternoon.
Above-described embodiment describes preferred implementation of the present invention; Be not that scope of the present invention is limited; Design under the prerequisite of spirit not breaking away from the present invention; Various distortion and improvement that the common engineers and technicians in this area make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (7)
1. method of utilizing microorganism pythium oligandrum radical cure apple canker is characterized in that: may further comprise the steps:
(1) at first with clear water cleaning morbidity trunk and branch, removes sticking supernatant germ and dust;
(2) preparation biocontrol microorganisms liquid: the pythium oligandrum microbial inoculum is poured in the container that fills a small amount of clear water; Soak and suitably stirring; Pour out supernatant after about 30 minutes; Continue to add clear water and dilute, add an amount of plant nutriment regulator, white sugar, urea and organosilicon auxiliary agent after the dilution again, stir and process biocontrol microorganisms liquid;
(3) treat biocontrol microorganisms liquid evenly to be sprayed after trunk and the branch dry tack free or be brushed on trunk and the branch.
2. method according to claim 1 is characterized in that: the every gram of said pythium oligandrum microbial inoculum contains 106 spores, adds said pythium oligandrum microbial inoculum 0.3 ~ 0.5 gram in every liter of biocontrol microorganisms liquid.
3. method according to claim 1 is characterized in that: said plant nutriment regulator is that plant Johnson & Johnson is plain or earthworm is fertile or the gibberellin or the basic element of cell division.
4. method according to claim 3 is characterized in that: the addition of said plant nutriment regulator is to add 1 ~ 4 milliliter or gram in every liter of biocontrol microorganisms liquid.
5. method according to claim 1 is characterized in that: the addition of said white sugar and urea is to add 10 gram white sugar and 10 gram urea in every liter of biocontrol microorganisms liquid.
6. method according to claim 1 is characterized in that: the addition of said organosilicon auxiliary agent is 0.1% of a biocontrol microorganisms liquid quality.
7. method according to claim 1 is characterized in that: said method begins control in the annual 5-8 month apple canker emergence period, uses once in later 15 ~ 30 days.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210344599.1A CN102835289B (en) | 2012-09-17 | 2012-09-17 | Method for radically treating apple cankers by microorganism pythium oligadrum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210344599.1A CN102835289B (en) | 2012-09-17 | 2012-09-17 | Method for radically treating apple cankers by microorganism pythium oligadrum |
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| CN102835289A true CN102835289A (en) | 2012-12-26 |
| CN102835289B CN102835289B (en) | 2014-06-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186076A (en) * | 2014-07-25 | 2014-12-10 | 刘镇 | Method for controlling apple rot by microorganism bacterial manure based on compound strains |
| CN107535524A (en) * | 2017-07-04 | 2018-01-05 | 天津市华宇农药有限公司 | The bactericidal composition of biological pesticide and its application |
| CN110072392A (en) * | 2016-10-14 | 2019-07-30 | 生物制品有限责任公司 | Liquid bio antifungal prod and preparation method containing pythium oligandrum microorganism |
| CN111996130A (en) * | 2020-09-21 | 2020-11-27 | 南京农业大学 | Biocontrol bacterium for plant root rot and application thereof |
-
2012
- 2012-09-17 CN CN201210344599.1A patent/CN102835289B/en not_active Expired - Fee Related
Non-Patent Citations (4)
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| 周尤马: "苹果腐烂病的综合防治", 《安徽农业》 * |
| 无: "浅谈腐烂病的发生与防治", 《百度文库》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186076A (en) * | 2014-07-25 | 2014-12-10 | 刘镇 | Method for controlling apple rot by microorganism bacterial manure based on compound strains |
| CN110072392A (en) * | 2016-10-14 | 2019-07-30 | 生物制品有限责任公司 | Liquid bio antifungal prod and preparation method containing pythium oligandrum microorganism |
| CN107535524A (en) * | 2017-07-04 | 2018-01-05 | 天津市华宇农药有限公司 | The bactericidal composition of biological pesticide and its application |
| CN111996130A (en) * | 2020-09-21 | 2020-11-27 | 南京农业大学 | Biocontrol bacterium for plant root rot and application thereof |
| CN111996130B (en) * | 2020-09-21 | 2022-02-01 | 南京农业大学 | Biocontrol bacterium for plant root rot and application thereof |
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| Publication number | Publication date |
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| CN102835289B (en) | 2014-06-18 |
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