CN102827930A - 建立幽门螺杆菌核酸指纹图谱的方法及其产品 - Google Patents
建立幽门螺杆菌核酸指纹图谱的方法及其产品 Download PDFInfo
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- CN102827930A CN102827930A CN2012102725336A CN201210272533A CN102827930A CN 102827930 A CN102827930 A CN 102827930A CN 2012102725336 A CN2012102725336 A CN 2012102725336A CN 201210272533 A CN201210272533 A CN 201210272533A CN 102827930 A CN102827930 A CN 102827930A
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Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
SEQ ID No:1 | 5-aggaagagagAGAGTTTGATCCTGGCTCAG-3(SEQ ID No:1) |
SEQ ID No:2 | 5-cagtaatacgactcactatagggagaaggctCTGCTGCGTCCCGTAG-3(SEQ ID No:2) |
PCR反应缓冲液(10x PCR Buffer with 20mM MgCl2) | 0.5ul |
dNTP mix(25mM each) | 0.04ul |
Taq酶(PCR Enzyme,5U/ul) | 0.04ul |
SEQ ID No:1(1μM) | 1ul |
SEQ ID No:2(1μM) | 1ul |
细菌DNA | 1ul |
超纯水 | 1.42ul |
RNase-free ddH2O | 0.5ul |
5xT7 Polymerase Buffer | 0.04ul |
T Cleavage Mix | 0.04ul |
DTT(100mM) | 1ul |
T7 RNA & DNA Polymerase | 1ul |
RNaseA | 1ul |
泳道 | 测试样品 | 目的片段 |
1 | 10 ml标准菌液 | ++ |
2 | 10ml待测鲜奶 | + |
3 | 10ml待测鲜奶 | + |
4 | 9ml鲜奶+1ml标准菌液 | + |
5 | 10ml无菌鲜奶对照 | - |
Claims (10)
Priority Applications (1)
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CN201210272533.6A CN102827930B (zh) | 2012-08-02 | 2012-08-02 | 建立幽门螺杆菌核酸指纹图谱的方法及其产品 |
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CN201210272533.6A CN102827930B (zh) | 2012-08-02 | 2012-08-02 | 建立幽门螺杆菌核酸指纹图谱的方法及其产品 |
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CN102827930A true CN102827930A (zh) | 2012-12-19 |
CN102827930B CN102827930B (zh) | 2014-10-08 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103103261A (zh) * | 2013-01-05 | 2013-05-15 | 张国新 | 检测幽门螺杆菌对克拉霉素耐药性试剂盒及制备方法 |
CN107037117A (zh) * | 2017-06-02 | 2017-08-11 | 华东医院 | 一种幽门螺杆菌的快速鉴定及毒力分析方法 |
-
2012
- 2012-08-02 CN CN201210272533.6A patent/CN102827930B/zh not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
李汉东 等: "《基质辅助激光解析/电离飞行时间质谱在微生物基因组研究中的应用》", 《济宁医学院学报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103103261A (zh) * | 2013-01-05 | 2013-05-15 | 张国新 | 检测幽门螺杆菌对克拉霉素耐药性试剂盒及制备方法 |
CN107037117A (zh) * | 2017-06-02 | 2017-08-11 | 华东医院 | 一种幽门螺杆菌的快速鉴定及毒力分析方法 |
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Publication number | Publication date |
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CN102827930B (zh) | 2014-10-08 |
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