CN102812804A - Accelerating germination method for shell-free type oil palm seeds - Google Patents
Accelerating germination method for shell-free type oil palm seeds Download PDFInfo
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- CN102812804A CN102812804A CN2012103262115A CN201210326211A CN102812804A CN 102812804 A CN102812804 A CN 102812804A CN 2012103262115 A CN2012103262115 A CN 2012103262115A CN 201210326211 A CN201210326211 A CN 201210326211A CN 102812804 A CN102812804 A CN 102812804A
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Abstract
The invention relates to an accelerating germination method for shell-free type oil palm seeds. The accelerating germination method comprises using abluent solution to soak the shell-free type oil palm seeds, and cleaning and airing the seeds; using a disinfectant to rinse the seeds, placing the seeds into a plump transparent sealing bag filled with air, adding pure water of 2-4ml into every 10 seeds, transferring sprouting seeds to a sand bed, enabling a sand layer with the thickness of 1-2cm to be covered on the sprouting seeds, maintaining moist, transplanting the seeds into a seedling growing bag filled with nutrition soil when the seedlings grow to 9-12cm on the sand bed, and enabling the seedlings to grow into strong seedlings. The accelerating germination method is simple in process, convenient to operate, low in cost and high in efficiency, and can effectively overcome the shortcomings that the shell-free seeds are easy to dry and are subjected to germ infection easily. Germination time is short, the germination rate, the seedling emergency and the strong seedling rate are high, and the method is efficient for accelerating germination and seedling growing of the shell-free type oil palm seeds.
Description
Technical field
The invention belongs to the seedling breeding technical field, relate to a kind of seed accelerating germination method, specifically is a kind of apheredermes oil palm seed accelerating germination method.
Background technology
Oil palm is important tropical oil crop, and its unit are oil production occupies first of the oil crop.Oil palm is divided into three kinds by the fruit type: thick shell mould, thin-shell type and apheredermes, three's characteristics are respectively the thick shell of seed, seed shell and seed does not have shell.Present stage, the oil palm of commercial cultivating and growing was the thin-shell type oil palm, be the crossbreed of thick shell mould and apheredermes, so the apheredermes oil palm had important function in the breeding of thin-shell type oil palm.
Thick shell mould and thin-shell type oil palm can avoid excessive dehydration and sick entomogenous fungi to infect, and owing to plant the protection of shell, germination accelerating method are very simple, directly sows the land for growing field crops such as seed and also can better sprout because its seed has kind of a shell protection.And apheredermes oil palm seed is in default of kind of a shell protection; Dehydration easily, germ is also infected embryo easily, and the germination accelerating method that is currently applied to thick shell mould and thin-shell type oil palm seed can not be applicable to apheredermes oil palm seed; Therefore, apheredermes oil palm seed is considered to extremely be difficult to sprout.Though under aseptic culture medium, cultivate and can overcome the sprouting problem through peeling off embryo, but experimental implementation is complicated, cost is higher, efficient is also low, and also there is the dormancy of certain hour in embryo under medium, causes the apheredermes oil palm seed germination time longer.
Summary of the invention
The purpose of this invention is to provide a kind of apheredermes oil palm seed accelerating germination method, can effectively overcome no shell seed and be prone to dehydration, be subject to shortcomings such as courses of infection, dormancy, realize quick, the neat sprouting of no shell oil palm seed, improve germination rate and planting percent.
The technical scheme that the present invention adopts is:
A kind of germination accelerating method of apheredermes oil palm seed, concrete steps are following:
1, seed separates: gather from the fresh palm fibre fruit of apheredermes oil palm tree and use plastic bag sealing, room temperature to deposit for 1～2 week, and soft mashed until pulp; Rinse well then; Remove all pulp and filament above the seed, used the abluent solution soaking then 0.5～1 hour, wash to remove grease with flowing water again; Dry at shady and cool place, for use.
2, presprouting of seeds: seed is used the thimerosal rinsing, carries out vernalization then; Germination accelerating method: with seed pack into heave, in the pneumatic transparent sealing bag, per 100 seeds add pure water 2～4ml, sealing places in 38～40 ℃ the insulating box then, waits for rudiment; In a single day seed germinates, and chitting piece is transferred on the husky bed at once.
In the presprouting of seeds process, said thimerosal is the liquor natrii hypochloritis of 1% concentration.
For preventing seed drying, fungi and bacterial infection seed, each week is with 0.5～1% concentration liquor natrii hypochloritis rinsing seed 1～2 time during the vernalization.
3, husky bed growth: on husky bed, cool canopy is set, and sterilizes with potassium permanganate in advance; Germinating seed is transferred on the husky bed, the sand bed of cladding thickness 1～2cm on germinating seed; Husky bed watered once to keep moistening in per 2～3 days; Seedling grows to high 9～12cm on husky bed, can transplant to the Seedling bag of adorning nutrition soil, makes it grow into strong sprout.
Technology of the present invention is simple; Easy to operate; Cost is low, and efficient is high, can effectively overcome no shell seed and be prone to shortcoming dry, that be subject to infection process; Realize that sprout time is short, germination rate is high, planting percent and strong sprout rate high, for the vernalization of apheredermes oil palm seed with grow seedlings a kind of high-efficiency method is provided.
Embodiment
Below in conjunction with embodiment, specific embodiments of the invention describes in further detail.Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment one
Get 150 seeds, with sterilization, divide the transparent sealing bag of putting into 3 long * wide=15cm * 10cm with liquor natrii hypochloritis's rinsing of 1% concentration; Add the 1.5ml pure water; The full gas of sealed bag drum, seal then, lie low and put in the insulating box; If 38 ℃ of temperature are waited for germinate (germination time is 2 months in the insulating box).Carry out operation below 1～2 time each week: seed is taken out, the liquor natrii hypochloritis's rinsing with 0.5%, and then put vernalization in the insulating box (if find the long bacterium, mouldy of seed, horse back takes out this seed and abandons) into.Seed promptly takes out and transfers on the husky bed once finding germination.The germination result sees table 1.
On husky bed, cool canopy is set, and sterilizes with potassium permanganate in advance; Germinating seed is transferred on the husky bed, the sand bed of cladding thickness 1cm on germinating seed; Husky bed watered once to keep moistening in per 2～3 days; Seedling grows to high 10cm on husky bed, can transplant to the Seedling bag of adorning nutrition soil, makes it grow into strong sprout.
Embodiment two
Get 150 seeds, with sterilization, divide the transparent sealing bag of putting into 3 long * wide=15cm * 10cm with liquor natrii hypochloritis's rinsing of 1% concentration; Add the 1.0ml pure water; The full gas of sealed bag drum, seal then, lie low and put in the insulating box; If 39 ℃ of temperature are waited for germinate (germination time is 2 months in the insulating box).Carry out operation below 1～2 time each week: seed is taken out, the liquor natrii hypochloritis's rinsing with 1%, and then put vernalization in the insulating box (if find the long bacterium, mouldy of seed, horse back takes out this seed and abandons) into.Seed promptly takes out and transfers on the husky bed once finding germination.The germination result sees table 1.
On husky bed, cool canopy is set, and sterilizes with potassium permanganate in advance; Germinating seed is transferred on the husky bed, the sand bed of cladding thickness 1.5cm on germinating seed; Husky bed watered once to keep moistening in per 2～3 days; Seedling grows to high 9cm on husky bed, can transplant to the Seedling bag of adorning nutrition soil, makes it grow into strong sprout.
Embodiment three
Get 150 seeds, with sterilization, divide the transparent sealing bag of putting into 3 long * wide=15cm * 10cm with liquor natrii hypochloritis's rinsing of 1% concentration; Add the 2.0ml pure water; The full gas of sealed bag drum, seal then, lie low and put in the insulating box; If 40 ℃ of temperature are waited for germinate (germination time is 2 months in the insulating box).Carry out operation below 1～2 time each week: seed is taken out, the liquor natrii hypochloritis's rinsing with 0.8%, and then put vernalization in the insulating box (if find the long bacterium, mouldy of seed, horse back takes out this seed and abandons) into.Seed promptly takes out and transfers on the husky bed once finding germination.The germination result sees table 1.
On husky bed, cool canopy is set, and sterilizes with potassium permanganate in advance; Germinating seed is transferred on the husky bed, the sand bed of cladding thickness 2cm on germinating seed; Husky bed watered once to keep moistening in per 2～3 days; Seedling grows to high 12cm on husky bed, can transplant to the Seedling bag of adorning nutrition soil, makes it grow into strong sprout.
The contrast experiment:
The contrast I: get 350 in seed, drilling is having (husky bed is sterilized with potassium permanganate in advance) on the husky bed of cool canopy, husky bed is watered to keep moistening in per 2～3 days, observes also record germination.The result sees table 1.
Contrast II: on superclean bench; Elder generation to seed disinfection 1min, with 0.1% mercury chloride sterilization 20min, uses rinsed with sterile water 3 times with 75% alcohol then again; Pry open kind of a benevolence with scalpel and tweezers at last; Take out embryo, be seeded on the MS minimal medium, under 27 ℃ of illumination cultivation chambers, cultivate then.Handle 50 embryos of inoculation.Observe and the record germination.The result sees table 1.
Table 1
Find out by the result, contrast I (husky bed is sproutung method directly), seed germination, one-tenth growth of cereal crop seedlings condition after 6 months, germination rate is low.Contrast II (medium culture method), after 2 months, a part of embryo germination, quite a few embryo also not to be sprouted, and there is dormancy in seed, sprouts inconsistent; Germination rate after 6 months increases, but the time is long.Germination hastening method of the present invention, basic vernalization success after 2 months, sprout time is short, germination rate is high, realizes quick, the neat sprouting of no shell oil palm seed, has higher germination rate and planting percent.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (3)
1. the germination accelerating method of an apheredermes oil palm seed is characterized in that concrete steps are following:
1), seed separates: collection uses plastic bag sealing, room temperature to deposit for 1～2 week from the fresh palm fibre fruit of apheredermes oil palm tree, and is soft mashed until pulp; Rinse well then; Remove all pulp and filament above the seed, used the abluent solution soaking then 0.5～1 hour, wash to remove grease with flowing water again; Dry at shady and cool place, for use;
2), presprouting of seeds: seed is used the thimerosal rinsing, carries out vernalization then; Germination accelerating method: with seed pack into heave, in the pneumatic transparent sealing bag, per 100 seeds add pure water 2～4ml, sealing places in 38～40 ℃ the insulating box then, waits for rudiment; In a single day seed germinates, and chitting piece is transferred on the husky bed at once;
3), husky bed growth: on husky bed, cool canopy is set, and sterilizes with potassium permanganate in advance; Germinating seed is transferred on the husky bed, the sand bed of cladding thickness 1～2cm on germinating seed; Husky bed watered once to keep moistening in per 2～3 days; Seedling grows to high 9～12cm on husky bed, can transplant to the Seedling bag of adorning nutrition soil, makes it grow into strong sprout.
2. germination accelerating method according to claim 1 is characterized in that: used thimerosal is the liquor natrii hypochloritis of 1% concentration in the said presprouting of seeds process.
3. germination accelerating method according to claim 1 is characterized in that: each week is with 0.5～1% concentration liquor natrii hypochloritis rinsing seed 1～2 time during the vernalization.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067802A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Method for increasing germination rate of nandina seeds |
| CN104488398A (en) * | 2014-11-14 | 2015-04-08 | 张家港市大新利群农民专业合作社 | Sweet pepper seeds activating treatment method |
| CN104904373A (en) * | 2015-06-25 | 2015-09-16 | 云南省热带作物科学研究所 | Fast germinating method of oil palm seed |
| CN105284406A (en) * | 2015-11-30 | 2016-02-03 | 金陵科技学院 | Simple accelerating germination technology for Cornus walteri seeds |
| CN107347295A (en) * | 2017-07-21 | 2017-11-17 | 中国科学院东北地理与农业生态研究所 | A kind of germination accelerating method using flat stalk cyperus iria L. rhizoma scirpi bulb nursery |
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| CN102084774A (en) * | 2010-11-05 | 2011-06-08 | 中国热带农业科学院椰子研究所 | Method for raising seedling of oil palm seed |
| CN102084775A (en) * | 2010-11-05 | 2011-06-08 | 中国热带农业科学院椰子研究所 | Method for growing seedlings of oil palm seeds |
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- 2012-09-06 CN CN2012103262115A patent/CN102812804A/en active Pending
Patent Citations (2)
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| CN102084774A (en) * | 2010-11-05 | 2011-06-08 | 中国热带农业科学院椰子研究所 | Method for raising seedling of oil palm seed |
| CN102084775A (en) * | 2010-11-05 | 2011-06-08 | 中国热带农业科学院椰子研究所 | Method for growing seedlings of oil palm seeds |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104067802A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Method for increasing germination rate of nandina seeds |
| CN104067802B (en) * | 2014-06-19 | 2016-01-06 | 安徽百卉园林有限公司 | A kind of method improving heavenly bamboo seed germination rate |
| CN104488398A (en) * | 2014-11-14 | 2015-04-08 | 张家港市大新利群农民专业合作社 | Sweet pepper seeds activating treatment method |
| CN104904373A (en) * | 2015-06-25 | 2015-09-16 | 云南省热带作物科学研究所 | Fast germinating method of oil palm seed |
| CN104904373B (en) * | 2015-06-25 | 2017-05-10 | 云南省热带作物科学研究所 | Fast germinating method of oil palm seed |
| CN105284406A (en) * | 2015-11-30 | 2016-02-03 | 金陵科技学院 | Simple accelerating germination technology for Cornus walteri seeds |
| CN105284406B (en) * | 2015-11-30 | 2018-08-14 | 金陵科技学院 | A kind of seed simple germination method of mao of Lai seed |
| CN107347295A (en) * | 2017-07-21 | 2017-11-17 | 中国科学院东北地理与农业生态研究所 | A kind of germination accelerating method using flat stalk cyperus iria L. rhizoma scirpi bulb nursery |
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Application publication date: 20121212 |