CN102805342A - Cordyceps militaris health-care product prepared through liquid subsurface fermentation and preparation method of cordyceps militaris health-care product - Google Patents
Cordyceps militaris health-care product prepared through liquid subsurface fermentation and preparation method of cordyceps militaris health-care product Download PDFInfo
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Abstract
The invention relates to a cordyceps militaris health-care product prepared through liquid subsurface fermentation and a preparation method of the cordyceps militaris health-care product, wherein the preparation method comprises the steps that A, cordyceps militaris bacteria are inoculated into culture liquid, in addition, the shaking culture is carried out on a shaking bed, and strain liquid is obtained; B, the stain liquid obtained in the step A is inoculated into a liquid culture medium containing barley malt and soybean, and after the aerated culture, fermented cordyceps militaris mycelia and cordyceps militaris bacterium fermentation liquid are obtained through fermentation; C, the cordyceps militaris mycelia obtained in the step B is extracted by water after cell tissue crushing, so cordyceps militaris mycelium extraction liquid is obtained; and D, the cordyceps militaris fermentation liquid obtained in the step B and the cordyceps militaris mycelium extraction liquid obtained in the step C are mutually mixed, and the obtained mixed liquid is concentrated, so the cordyceps militaris health-care product is obtained. The industrial liquid subsurface fermentation technology is adopted, and the cordyceps militaris stains can realize the industrial and massive propagation in the liquid culture medium, so the process is simple, and the cost is lower.
Description
Technical field
The present invention relates to a kind of health products and preparation method thereof, particularly relate to a kind of Chinese caterpillar fungus health article that pass through the liquid deep layer fermenting preparation and preparation method thereof.
Background technology
Cordyceps militaris (formal name used at school: Cordycepsmilitaris) be Ascomycotina, ergot Zoopagales, Clavicipitaceae, Cordyceps.Its universal natural resources quantity seldom.By stroma (being careless part) and sclerotium (being the corpse part of worm) complex dimerous.Winter, larva lived in seclusion in the soil, and the mushroom parasitism is wherein drawn nutrition, was full of mycelia in the larva body and dead.To summer, on the larva corpse, bear seedling, the likeness in form grass, front and back were gathered and were got the Summer Solstice.The traditional Chinese medical science thinks that Cordyceps militaris goes into lung kidney two warps, and is can tonifying lung cloudy, again can kidney-replenishing, and cure mainly and suffer from a deficiency of the kidney, impotence and seminal emission, soreness of waist and knee joint, weak after being ill, chronic cough is weak, phthisical cough phlegm blood, spontaneous sweatings etc. are unique a kind of balances simultaneously, regulate the Chinese medicine of negative and positive.
At present, correlative study both domestic and external mainly concentrates on extraction and pharmacology, the drug effect of Cordyceps militaris function factor.But the artificial cultivation field at fruiting bodies of cordyceps militaris but receives a lot of technical limitations, carries out suitability for industrialized production and civil nature use widely seldom on a large scale, and simultaneously, this technical field also is difficult to commercialization and uses.Before 10 years, the year recoverable amount of occurring in nature fruiting bodies of cordyceps militaris has 10,000 tons approximately, 5 kilotons but present recoverable amount is but only had an appointment; And; As stated, fruiting bodies of cordyceps militaris is difficult to a large amount of manual work production again owing to technical reason; Therefore, people have produced significant contradiction to the shortage of craving for the fruiting bodies of cordyceps militaris recoverable amount of Chinese caterpillar fungus health effect and therapeutic efficiency.
Summary of the invention
The objective of the invention is to; Overcome the defective of above-mentioned prior art and a kind of suitability for industrialized production of can carrying out on a large scale is provided, and can produce the method that liquid deep layer fermenting prepares the Chinese caterpillar fungus health article of passing through of the higher fruiting bodies of cordyceps militaris of effectiveness with lower cost.
For realizing above-mentioned purpose, the present invention takes following design:
Of the present inventionly a kind ofly prepare the method for Chinese caterpillar fungus health article, comprise the steps: through liquid deep layer fermenting
A, cordyceps is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation;
C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains cordyceps filament extract;
D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain the Chinese caterpillar fungus health article.
Preferably, in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
More preferably, the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
More preferably, in said step D, the mutual mixed pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
Another object of the present invention is to provide a kind of Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation, said Chinese caterpillar fungus health article are to be prepared from following method:
A, cordyceps is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation;
C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains cordyceps filament extract;
D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain the Chinese caterpillar fungus health article.
Preferably, in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
More preferably, the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
More preferably, in said step D, the mutual mixed pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
Advantage of the present invention is: the present invention adopts that the cordyceps in bacterial classification original producton location separates, purifying and cultivation; Adopt industrialization liquid deep layer fermenting technology, make Cordyceps militaris spawn to carry out a large amount of breedings in the liquid medium within, and; All contain in the metabolite of gained and the mycelium and the identical active ingredient of former cordyceps effectiveness; In addition, the present invention also makes the fructification of cordyceps to produce and to cultivate in industrialized condition, and technology is simple, cost is controlled; Thereby can reduce production costs significantly, make ordinary consumer also can use famous and precious health product.
The specific embodiment
Embodiment 1:
Following prepare the preferred embodiment of the method for Chinese caterpillar fungus health article for the present invention is a kind of through liquid deep layer fermenting, preparation method of the present invention comprises following key step:
A, at first; Cordyceps is inserted in the nutrient solution; Nutrient solution of the present invention can be the various nutrient solutions that are suitable for the cordyceps Liquid Culture of the prior art, and under 18 ℃ temperature, on shaking table, shakes cultivation 72 hours with 300 times/minute concussion frequency, obtains strain liquid; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 8% fructus hordei germinatus, weight portion 6% soybean, and inoculum concentration is 5%, and ventilation was cultivated 48 hours under 27 ℃ temperature; Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation; C, again the cordyceps filament that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.8:5, extraction temperature is 95 ℃, extraction time is 2 hours, thereby obtains cordyceps filament extract; Further, Cordyceps militaris zymotic fluid and wet thallus that the present invention mainly utilizes industrialization liquid deep layer fermenting technology to obtain prepare and produce Chinese caterpillar fungus health article or health drink; D, last after the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other, concentrates 4 times with the mixed liquor of gained, and makes its refractive power pol reach 10%, thereby obtain the Chinese caterpillar fungus health article.Therefore,, popular straight drink product be can produce, health products and beverage comprised through preparation method of the present invention.And preparation method of the present invention can directly apply to suitability for industrialized production, and cost is low, and the products obtained therefrom trophic function is high, helps consumer and public daily health carings.
More specifically, preparation method of the present invention can directly carry out industrial and large-scale and produces continuously.After the batch process, can produce the cordyceps zymotic fluid more than 10 tons every day, and produce 20 tons～final products more than 50 tons every day through preparation method of the present invention.
On the other hand, the method for concentration that in preparation method's of the present invention step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained Chinese caterpillar fungus health article, the content of its functional components and fruiting bodies of cordyceps militaris are close or be higher than fruiting bodies of cordyceps militaris, and its effectiveness is with directly to take Cordyceps militaris suitable.
More preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) are preferably and reach relevant parameters separately simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed natural pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
The content of functional component of the inventive method products therefrom and quality are all highly stable, and are higher than modern pharmacology and research and analyse the numerical value scope of determination to natural Cordyceps militaris component content.Preparation method's of the present invention industrialization control ability is strong, and its cost is far below natural material.
Embodiment 2:
Preparation method's of the present invention another kind of preferred embodiment will be provided as follows.The preparation method of present embodiment comprises: A, at first, select food, medicinal bacterium Cordyceps militaris for use, and cordyceps is inserted in the nutrient solution, and under 28 ℃ temperature, on shaking table, shake and cultivated 96 hours with 200 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 12% fructus hordei germinatus, weight portion 3% soybean; Inoculum concentration is 10%; And ventilation was cultivated 72 hours under 23 ℃ temperature, through biofermentation, obtained the cordyceps filament and the cordyceps zymotic fluid of fermentation; C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 1:2, extraction temperature is 90 ℃, extraction time is 3 hours, thereby obtains cordyceps filament extract; D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 7 times, and makes its refractive power pol reach 12%, thereby obtain the Chinese caterpillar fungus health article.
Embodiment 3:
Another preferred embodiment of preparation method of the present invention will be provided as follows.The preparation method of present embodiment comprises the steps: A, cordyceps is inserted in the nutrient solution, and under 22 ℃ temperature, on shaking table, shakes cultivation 80 hours with 260 times/minute concussion frequency, obtains strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 10% fructus hordei germinatus, weight portion 5% soybean; Inoculum concentration is 7%; And ventilation was cultivated 60 hours under 25 ℃ temperature, through biofermentation, obtained the cordyceps filament and the cordyceps zymotic fluid of fermentation; C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.9:4, extraction temperature is 93 ℃, extraction time is 2.5 hours, thereby obtains cordyceps filament extract; D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 6 times, and makes its refractive power pol reach 11%, thereby obtain the Chinese caterpillar fungus health article.
Embodiment 4:
Present embodiment provides a kind of Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation; Said Chinese caterpillar fungus health article are to be prepared from following method: A, will select for use food, medicinal cordyceps to insert in the nutrient solution; And under 18～28 ℃ temperature (present embodiment is preferably 20 ℃), on shaking table, shake 72～96 hours (present embodiment is preferably 82 hours) of cultivation with the concussion frequency of 200～300 times/minute (present embodiment is preferably 260 times/minute), obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% (present embodiment is preferably 11%) fructus hordei germinatus, weight portion 3～6% (present embodiment is preferably 5%) soybean; Inoculum concentration is 5～10% (present embodiment is preferably 8%); And ventilation is cultivated 48～72 hours (present embodiment is preferably 56 hours under the temperature of 23～27 ℃ (present embodiment is preferably 26 ℃); Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation; C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after; Water extracts; The lixiviate ratio is 0.8～1:2～5 (present embodiment is preferably 1:3); Extraction temperature is 90～95 ℃ (present embodiment is preferably 92 ℃), and extraction time is 2.5 hours, thereby obtains cordyceps filament extract; D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain the Chinese caterpillar fungus health article.
Wherein, the method for concentration that in step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained Chinese caterpillar fungus health article, its effectiveness is with directly to take Cordyceps militaris suitable.
Preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) reach corresponding separately index simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
Other embodiment of a kind of Chinese caterpillar fungus health article through liquid deep layer fermenting preparation of the present invention are identical or close with the foregoing description 1,2 and 3, repeat no more at this.To those skilled in the art, be not difficult from the foregoing description 1,2 and 3, to obtain relevant teachings, and in order to preparation or production Chinese caterpillar fungus health article.
Obviously, those of ordinary skill in the art can use a kind of Chinese caterpillar fungus health article that pass through the liquid deep layer fermenting preparation of the present invention and preparation method thereof, forms various types of health products and preparation method thereof.
The foregoing description only supplies to explain the present invention's usefulness; And be not to be limitation of the present invention; The those of ordinary skill in relevant technologies field without departing from the present invention, can also be made various variations and modification; Therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited each claim.
Claims (10)
1. prepare the method for Chinese caterpillar fungus health article through liquid deep layer fermenting, it is characterized in that: said preparation method comprises the steps:
A, cordyceps is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation;
C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains cordyceps filament extract;
D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain the Chinese caterpillar fungus health article.
2. according to claim 1ly prepare the method for Chinese caterpillar fungus health article, it is characterized in that: in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration through liquid deep layer fermenting.
3. according to claim 2ly prepare the method for Chinese caterpillar fungus health article through liquid deep layer fermenting, it is characterized in that: the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
4. the method for preparing the Chinese caterpillar fungus health article through liquid deep layer fermenting according to claim 1; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
5. the method for preparing the Chinese caterpillar fungus health article through liquid deep layer fermenting according to claim 4; It is characterized in that: in said step D; The mutual mixed pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
6. one kind is passed through the Chinese caterpillar fungus health article that liquid deep layer fermenting prepares, and it is characterized in that: said Chinese caterpillar fungus health article are to be prepared from following method:
A, cordyceps is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the cordyceps filament and the cordyceps zymotic fluid of fermentation;
C, the cordyceps filament that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains cordyceps filament extract;
D, the cordyceps filament extract that obtains among cordyceps zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain the Chinese caterpillar fungus health article.
7. the Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation according to claim 6 is characterized in that: in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
8. the Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation according to claim 7, it is characterized in that: the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
9. the Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation according to claim 6; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
10. the Chinese caterpillar fungus health article through the liquid deep layer fermenting preparation according to claim 9, it is characterized in that: in said step D, the mutual mixed pH value of cordyceps zymotic fluid and cordyceps filament extract is 6～7, and the refractive power pol is 2～6%.
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Cited By (2)
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| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
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| CN102389145A (en) * | 2011-11-12 | 2012-03-28 | 陈宏伟 | Method for preparing composite beverage by fermenting Cordyceps militaris |
| CN102389139A (en) * | 2011-09-30 | 2012-03-28 | 河北师范大学 | Preparation method for edible fungus nutritional health-care functional drink |
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| KR20000018849A (en) * | 1998-09-05 | 2000-04-06 | 임양빈 | Health supplementation foods using cordyceps militaris and production process |
| CN1507772A (en) * | 2002-12-16 | 2004-06-30 | 北京市食品研究所 | Method for preparing hickory chick by liquid deep fermentation and product thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN103330258B (en) * | 2013-07-17 | 2014-09-10 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
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