CN102792973A - Compound bactericide and preparation method thereof - Google Patents
Compound bactericide and preparation method thereof Download PDFInfo
- Publication number
- CN102792973A CN102792973A CN2012102637015A CN201210263701A CN102792973A CN 102792973 A CN102792973 A CN 102792973A CN 2012102637015 A CN2012102637015 A CN 2012102637015A CN 201210263701 A CN201210263701 A CN 201210263701A CN 102792973 A CN102792973 A CN 102792973A
- Authority
- CN
- China
- Prior art keywords
- root
- paecilomyces lilacinus
- weight portion
- compound
- chinese stellera
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a compound bactericide prepared from trichoderma koningii, paecilomyces lilacinus, Chinese stellera root and marigold, and a preparation method thereof. The compound biopesticide bactericide uses trichoderma koningii, paecilomyces lilacinus, Chinese stellera root and marigold as main components, and is prepared through the following steps: producing trichoderma koningii and paecilomyces lilacinus compound fungicide by a dual-temperature solid fermentation method; and mixing the trichoderma koningii and paecilomyces lilacinus compound fungicide with crushed Chinese stellera root and marigold complete stool in proportion. The compound biopesticide bactericide has the advantages of simple preparation process, low preparation cost, wide sterilization range, good comprehensive prevention and control effect and pathogen drug resistance prevention, and can be widely applied in the fields of agriculture, forestry, livestock farming and the like.
Description
Technical field
The present invention relates to biopesticide, be specially a kind of composite bactericide of processing by koning trichoderma, Paecilomyces lilacinus and Chinese Stellera Root, Aztec marigold and preparation method thereof.
Background technology
Trichoderma (
Trichoderma spp.) belong to Fungi Imperfecti Hyphomycetes (
Hyphomycetes) Moniliales (
Moniliales) Moniliaceae (
Moniliaceae), extensively being present in the environment such as soil, the mould antagonism property chemical substance that in metabolic process, can produce of wood is come the poisonous plant disease fungus.Many trichoderma strains produce volatility or nonvolatile antibiotic class material, and trichodermin (trichodermin), gliotoxin (gliotoxin), viridin (viridin), antibacterial peptide (peptideantibiotic) etc. are arranged.Since the kind of antibiotics, the difference of the chemical property and the mode of action, and pathogen often is difficult to develop pesticide resistance.Paecilomyces lilacinus [Paecilornyces lilacinus (Thom.) Samson] is a ubiquitous saprophytic bacteria in soil and the plant; Ovum to root-knot nematode and cyst roundworm has very strong parasitic ability; Be cause principal element that root-knot nematode and cyst roundworm fail naturally it, the mould microbial inoculum can be used as the biological control of various plants nematode.Chinese Stellera Root (
Stellera chamaej asme L.) be Thymelaeceae stellera plant, the another name root of langdu, gelsemium elegan etc., its root is the matrix of the traditional Chinese medicine root of langdu.The in-vitro antibacterial test shows that the root of langdu all has very strong vitro inhibition effect to multiple bacillus, coccus and fungi.As botanical pesticide, have been found that the root of langdu has multiple insecticidal activity.The feverfew Aztec marigold (
Tagetes patula), Dicotyledoneae, all there is plantation in many in the world countries as ornamental plants.In recent years, this extract is causing concern widely aspect the bioscience research.Both at home and abroad nematicide, the insect active to the chemical composition of Aztec marigold such as thiophene-based, essential oil, flavones etc. has the research report.
In order to overcome many defectives that these single type bactericide exist; People can carry out reasonably combination collocation to some for compatible biopesticide usually; Process various composite bactericides, various effective germicide mass-energy are strengthened each other, replenish each other; Thereby reach bactericidal range, the enhancing sterilizing ability of widening biopesticide; Compare with the bactericide of single type, therefore characteristics such as production cost is low, bactericidal effect good, using dosage is little, pathogen is difficult for developing immunity to drugs that it has have vast potential for future development.
Summary of the invention
The objective of the invention is to be to provide composite bactericide and the production method thereof that a kind of production cost is low, bactericidal range is wide, integrated control effect is good.
Concrete technical scheme of the present invention is, a kind of composite bactericide and production method thereof, and primary raw material does, koning trichoderma, Paecilomyces lilacinus and Chinese Stellera Root, Aztec marigold, auxiliary material is emulsifier, anti-caking agent, anti-drift agent.
Through koning trichoderma and Paecilomyces lilacinus are seeded in the medium top fermentation; Produce koning trichoderma and Paecilomyces lilacinus composite bacteria agent capable; Through crushed after being dried, again with pulverize after Chinese Stellera Root root, Aztec marigold complete stool and auxiliary material mix by proportioning, the proportioning of said each component is:
Koning trichoderma and Paecilomyces lilacinus solid culture compound 60-80 weight portion.
Chinese Stellera Root root 10-20 weight portion
Aztec marigold complete stool 5-15 weight portion
Emulsifier 1-3 weight portion
Anti-caking agent 1-2 weight portion
Anti-drift agent 0.5-1.0 weight portion.
Emulsifier is that alkylaryl APEO, APEO, lignosulfonates, alkylnaphthalene sulfonate are any; Anti-caking agent is that alkylsulfonate, alkylnaphthalene sulfonate, naphthalene sulfonate are any; Anti-drift agent is that glycerine, ethylene glycol, atoleine are any.
The invention provides a kind of efficient, low toxicity, low-residual, with the harmonious new bio source pesticide of environment, its to the bactericidal range of widening biopesticide, strengthen sterilizing ability, reduce the amount of application of chemical pesticide, reduce residue of pesticide and all have far reaching significance.Compare with pesticides prepared by chemistry, the present invention has following characteristics and advantage:
(1) raw material sources are extensive, production cost is low, production technology is simple.
(2) bactericidal range is extensive, can well kill and inhibitory action causing that pathogens such as root rot, damping off, damping off, bacterial wilt, fusarium wilt, verticillium wilt, root-knot nematode have.
(3) control efficiency is remarkable, medication after three days control efficiency can reach more than 90%.
(4) dosage is few, and the drug effect phase is long, and integrated control effect is good, and pathogen is difficult to develop immunity to drugs.
Embodiment
Embodiment 1
1 test place: greenhouse, Taiyuan, Shanxi suburb canopy tomato
2 test periods: in October, 2011
3 controlling objects: root-knot nematode
4 schemes:
Two temperature solid state fermentation production technology of the present invention is:
1, medium is formed
Slant culture: potato 20%, glucose 2%, agar 2%.
Shake-flask culture base: potato 20%, glucose 2%, yeast extract 1%.
Solid culture medium: straw powder 50%, (NH4)
2SO
4About 8-9%, wheat bran is about 30%, corn flour 10%, glucose 1%, Tween800.4%, the ratio 4.8-4.9:1 of water and medium, medium PH nature (about 6.5).
2, medium sterilization: put into autoclave after medium mixes according to proportioning and sterilize, 121 ℃ of sterilizations of temperature were sterilized 40-60 minute in 20 minutes or 100 ℃.
3, koning trichoderma slant culture and shake-flask culture: slant culture: in the test tube of slant medium, insert koning trichoderma, 30 ℃ leave standstill cultivation 3-5d; Shake-flask culture: the amount of shaking in the bottle with 10% at the 1000ml of shake-flask culture base 200ml inserts the koning trichoderma spore suspension, under 25 ℃, 120-150r/min condition, cultivates 2d.
4, Paecilomyces lilacinus slant culture and shake-flask culture: slant culture: in the test tube of slant medium, insert Paecilomyces lilacinus, 25 ℃ leave standstill cultivation 2-4d; Shake-flask culture: the amount of shaking in the bottle with 8% at the 1000ml of shake-flask culture base 200ml inserts the Paecilomyces lilacinus spore suspension, under 25 ℃, 100-110r/min condition, cultivates 24h.
5, koning trichoderma inoculation fermentation: with 10% amount handled from the inoculation of shake-flask culture base, again with medium at the thin layer that is tiled on the shallow tray fermentation frame about 2 centimetres, 30～32 ℃ of preceding 30h constant temperature, back 42h are kept 27 ℃; Natural supply oxygen, ambient humidity are controlled at about more than 60%.
6, Paecilomyces lilacinus inoculation fermentation: koning trichoderma fermentation 72h, stable growth after date inoculation Paecilomyces lilacinus is inoculated from the shake-flask culture base with the amount of 20% processing; Keeping temperature between solid fermentation is 27 ℃, and it is 70%-80% that fermentation 1-3d keeps the humidity in the sweathouse, from 4d; Needn't humidification, keep the humidity 10%-40% under the home, when fermentation 3-4d, give on the solid fermentation medium and spray 20% aseptic glucose solution once; Fermentation 7d; Spore is ripe, and solid culture medium forms the bacterium piece, and then solid fermentation finishes.
7, drying: after the fermentation ends, medium is carried out vacuum cooling drying.
8, pulverize: the medium powder of drying is broken into the powder below 300 orders, the packing storage.
Complex biological pesticide sterilizing agent compounding method of the present invention: Chinese Stellera Root root, Aztec marigold complete stool and medium are ground into the fine powder below 300 orders respectively; Add in the mixer, add emulsifier, anti-caking agent successively, with the rotating speed mixing of 150-200r/min 40-60 minute.
The ratio of described each raw material of composite bactericide of present embodiment is:
Koning trichoderma and Paecilomyces lilacinus solid culture compound 70 weight portions.
Chinese Stellera Root root 15 weight portions
Aztec marigold complete stool 10 weight portions
Alkylaryl APEO 0.5 weight portion
Lignosulfonates 1.5 weight portions
Alkylnaphthalene sulfonate, 1.5 weight portions
Atoleine, 0.8 weight portion
5. experimental scheme:
It is fertile to choose soil property in the suburb, Taiyuan, and the uniform tomato booth of soil fertility carries out tomato in October, 2011 and plants.After composite bactericide and fine earth ratio 1:10 mix, before tomato is transplanted, the every tomato root system placed around 20-30 of section gram medicament; After perhaps microbial inoculum and fine earth 1:100 mixed, full garden was spread fertilizer over the fields the back and is transplanted tomato.With two kinds of methods the tomato field is handled respectively.
This tests every sub-district is 2 ridges, and 3 repetitions are set.Handle and the contrast randomized arrangement.Treat to pluck measurement behind the fruit maturation, and it is right to survey product according to the sub-district area, selects for use the line of selling on the market extremely red according to medicament.
6 test effects:
Table 1 root drop-test effect
| ? | Product | The contrast medicament | The clear water contrast | Increase percentage (%) |
| Flowering time | November 8 | November 16 | November 20 | ? |
| Eelworm harm rate (%) | 17.5 | 32.6 | 60.2 | 42.70 |
| Per mu yield (kg) | 10371.8 | 8331.6 | 6732.2 | 54.06 |
Table 2 is mixed soil and is broadcasted sowing test effect
| ? | Product | The contrast medicament | The clear water contrast | Increase percentage (%) |
| Flowering time | November 9 | November 15 | November 21 | ? |
| Eelworm harm rate (%) | 16.3 | 42.7 | 65.3 | 49.00 |
| Per mu yield (kg) | 10426.8 | 7942.5 | 6426.3 | 62.25 |
Under 2 kinds of occupation modes, this product all has the better prevention effect to root-knot nematode.After wherein root was thrown in, the diseased plant number was than clear water contrast low 42.70%; Mixing soil broadcasts sowing than clear water contrast low 49%.Simultaneously, find in investigation that can effectively shift to an earlier date the tomato florescence after using this product, root is thrown in and mixed soil and broadcast sowing equal can shifting to an earlier date about about 12d of tomato florescence.Produce discovery through final the survey, this product can effectively improve tomato yield, and root is thrown in and mixed soil and broadcast sowing the tomato production promoting 54.6% and 62.25% that can make eelworm harm respectively, can effectively reduce the plantation risk at plantation family.
Embodiment 2:
1 test place: greenhouse, Taiyuan, Shanxi suburb canopy cucumber
2 test periods: in October, 2011
3 controlling objects: fusarium wilt, damping off, damping off
4 schemes: the medium fermentation process is with embodiment 1,
Koning trichoderma and Paecilomyces lilacinus solid culture compound 60 weight portions.
Chinese Stellera Root root 20 weight portions
Aztec marigold complete stool 5 weight portions
Emulsifier 3 weight portions
Anti-caking agent 1 weight portion
Anti-drift agent 1.0 weight portions.
Emulsifier is an APEO; Anti-caking agent is an alkylsulfonate; Anti-drift agent is a glycerine.
5 experimental scheme:
It is fertile to choose soil property in the suburb, Taiyuan, and the uniform booth of soil fertility carries out cucumber in October, 2011 and plants.After composite bactericide and fine earth ratio 1:10 mix, before cucumber is transplanted, every cucumber root placed around 20-30 gram medicament; After perhaps microbial inoculum and fine earth 1:100 mixed, full garden was spread fertilizer over the fields the back and is transplanted cucumber.With two kinds of methods the cucumber field is handled respectively.
This tests every sub-district is 2 ridges, and 3 repetitions are set.Handle and the contrast randomized arrangement.Treat to pluck measurement behind the fruit maturation, and survey product according to the sub-district area, the contrast medicament is selected the carbendazim of selling on the market for use.
6 test effects:
Table 3 root drop-test effect
| ? | Product | The contrast medicament | The clear water contrast | Preventive effect (%) |
| The fusarium wilt number | 0.8 | 4.2 | 9.3 | 91.40 |
| Damping off morbidity number | 1.3 | 6.5 | 10.6 | 87.74 |
| Damping off morbidity number | 0.6 | 5.3 | 8.2 | 92.68 |
Table 4 is mixed soil and is broadcasted sowing test effect
| ? | Product | The contrast medicament | The clear water contrast | Preventive effect (%) |
| The fusarium wilt number | 0.9 | 5.5 | 10.2 | 91.18 |
| Damping off morbidity number | 1.2 | 6.2 | 9.5 | 87.37 |
| Damping off morbidity number | 0.8 | 5.2 | 10.7 | 92.52 |
Find that through test this product all has preventive effect preferably to cucumber fusarium axysporum, damping off and damping off.Find that in seedling stage investigation 2 kinds of methods for using all reach more than 90% for the preventive effect of fusarium wilt and damping off, also can be more than 87% for the preventive effect of damping off.
Embodiment 3:
1 test place: cassava garden, Taiyuan, Shanxi suburb
2 test periods: in October, 2011
3 controlling objects: to the influence of cassava growth and piece root output
4 prescriptions: the medium fermentation process is with embodiment 1,
Koning trichoderma and Paecilomyces lilacinus solid culture compound 80 weight portions.
Chinese Stellera Root root 10 weight portions
Aztec marigold complete stool 15 weight portions
Emulsifier 1 weight portion
Anti-caking agent 2 weight portions
Anti-drift agent 0.5 weight portion.
Emulsifier is an APEO; Anti-caking agent is a naphthalene sulfonate; Anti-drift agent is a propane diols.
5 experimental scheme
It is fertile to choose soil property in the suburb, Taiyuan, and the uniform cassava of soil fertility garden is carried out tomato in October, 2011 and planted.After composite bactericide and fine earth ratio 1:10 mix, before cassava is transplanted, every cassava root system placed around 20-30 gram medicament; After perhaps microbial inoculum and fine earth 1:100 mixed, full garden was spread fertilizer over the fields the back and is transplanted cassava.With two kinds of methods cassava is handled respectively.7d and 14d time investigation emergence rate after plantation.Measure stem stalk rugosity when treating fruit maturation, and survey product according to plucking area, the contrast medicament is selected the plant protection of market sale for use.
6 test effects:
Table 5 root drop-test effect
| ? | Product | The contrast medicament | The clear water contrast | Increase percentage (%) |
| 7d emergence rate (%) | 90.23 | 85.42 | 78.64 | 11.59 |
| 14d emergence rate (%) | 97.22 | 88.34 | 83.37 | 13.85 |
| Stem stalk rugosity (cm) | 3.2 | 2.8 | 2.5 | 28.00 |
| Per mu yield (kg) | 654.8 | 612.7 | 582.4 | 12.43 |
Table 6 is mixed soil and is broadcasted sowing test effect
| ? | Product | The contrast medicament | The clear water contrast | Increase percentage (%) |
| 7d emergence rate (%) | 91.02 | 86.32 | 76.24 | 14.78 |
| 14d emergence rate (%) | 96.54 | 89.41 | 85.37 | 11.17 |
| Stem stalk rugosity (cm) | 3.2 | 2.6 | 2.4 | 33.33 |
| Per mu yield (kg) | 663.7 | 607.6 | 568.1 | 16.83 |
After using medicine fertilizer, to the outstanding effect that significantly promotes of cassava emergence rate, 7d and 14d emergence rate are all more than 90%, apparently higher than contrast and medicament contrast; The plant cane of each treatment region reaches maximum during maturing stage of cassava, is 3.2cm, and the sturdy growth of cassava cane is had certain promotion effect, shows that this product can effectively promote cassava plant growth of later stage.Medicine is fertile handles comparison respectively according to raising the output 12.43%, 16.83%.
Claims (3)
1. composite bactericide and production method thereof, primary raw material do, koning trichoderma, Paecilomyces lilacinus and Chinese Stellera Root, Aztec marigold, and auxiliary material is emulsifier, anti-caking agent, anti-drift agent,
Through koning trichoderma and Paecilomyces lilacinus are seeded in the medium top fermentation; Produce koning trichoderma and Paecilomyces lilacinus composite bacteria agent capable; Through crushed after being dried, again with pulverize after Chinese Stellera Root root, Aztec marigold complete stool and auxiliary material mix by proportioning, the proportioning of said each component is:
Koning trichoderma and Paecilomyces lilacinus solid culture compound 60-80 weight portion
Chinese Stellera Root root 10-20 weight portion
Aztec marigold complete stool 5-15 weight portion
Emulsifier 1-3 weight portion
Anti-caking agent 1-2 weight portion
Anti-drift agent 0.5-1.0 weight portion.
2. composite bactericide according to claim 1 and production method thereof is characterized in that: emulsifier is that alkylaryl APEO, APEO, lignosulfonates, alkylnaphthalene sulfonate are any; Anti-caking agent is that alkylsulfonate, alkylnaphthalene sulfonate, naphthalene sulfonate are any; Anti-drift agent is that glycerine, ethylene glycol, atoleine are any.
3. composite bactericide according to claim 1 and production method thereof is characterized in that:
Koning trichoderma and Paecilomyces lilacinus solid culture compound 70 weight portions
Chinese Stellera Root root 15 weight portions
Aztec marigold complete stool 10 weight portions
Alkylaryl APEO 0.5 weight portion
Lignosulfonates 1.5 weight portions
Alkylnaphthalene sulfonate, 1.5 weight portions
Atoleine, 0.8 weight portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210263701.5A CN102792973B (en) | 2012-07-28 | 2012-07-28 | Compound bactericide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210263701.5A CN102792973B (en) | 2012-07-28 | 2012-07-28 | Compound bactericide and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102792973A true CN102792973A (en) | 2012-11-28 |
| CN102792973B CN102792973B (en) | 2014-04-09 |
Family
ID=47192479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210263701.5A Expired - Fee Related CN102792973B (en) | 2012-07-28 | 2012-07-28 | Compound bactericide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102792973B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609616A (en) * | 2013-11-11 | 2014-03-05 | 李文斌 | Botanic fungicide and preparation method thereof |
| CN105594767A (en) * | 2016-03-07 | 2016-05-25 | 鞍山禾瑞生物科技有限公司 | Liquid state composite biological multivariable control paddy herbicide and preparation method thereof |
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| CN105831175A (en) * | 2016-04-29 | 2016-08-10 | 鞍山禾瑞生物科技有限公司 | Liquid compound biological multi-control fungicide and preparation method and application thereof |
| CN106212122A (en) * | 2016-07-26 | 2016-12-14 | 宾阳县华信种植专业合作社 | A kind of method preventing and treating Fructus Gardeniae grandiflorae daughter root maize ear rot |
| CN112940946A (en) * | 2021-01-27 | 2021-06-11 | 贵州大学 | Method for preparing paecilomyces lilacinus from marigold straws to prevent and control tobacco root-knot nematodes |
| TWI793074B (en) * | 2016-04-28 | 2023-02-21 | 日商組合化學工業股份有限公司 | Pesticide formulation composition using trichoderma, method of production thereof, and application method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439270A (en) * | 2002-02-20 | 2003-09-03 | 汪洪 | Preparing method for lilacinin against nematoda eggs |
| CN101019557A (en) * | 2007-01-26 | 2007-08-22 | 北京天地绿园农业科学研究院 | Recipe and prepn of botanical composite pesticide |
| CN101220335A (en) * | 2007-12-28 | 2008-07-16 | 中国烟草总公司郑州烟草研究院 | Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof |
| CN102428966A (en) * | 2011-09-29 | 2012-05-02 | 山东靠山生物科技有限公司 | Composite bio-formulation for preventing crop diseases and application thereof |
-
2012
- 2012-07-28 CN CN201210263701.5A patent/CN102792973B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1439270A (en) * | 2002-02-20 | 2003-09-03 | 汪洪 | Preparing method for lilacinin against nematoda eggs |
| CN101019557A (en) * | 2007-01-26 | 2007-08-22 | 北京天地绿园农业科学研究院 | Recipe and prepn of botanical composite pesticide |
| CN101220335A (en) * | 2007-12-28 | 2008-07-16 | 中国烟草总公司郑州烟草研究院 | Bacterial strain for preventing and controlling tobacco black shank and bacterium agent thereof |
| CN102428966A (en) * | 2011-09-29 | 2012-05-02 | 山东靠山生物科技有限公司 | Composite bio-formulation for preventing crop diseases and application thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609616A (en) * | 2013-11-11 | 2014-03-05 | 李文斌 | Botanic fungicide and preparation method thereof |
| CN105594767A (en) * | 2016-03-07 | 2016-05-25 | 鞍山禾瑞生物科技有限公司 | Liquid state composite biological multivariable control paddy herbicide and preparation method thereof |
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| TWI793074B (en) * | 2016-04-28 | 2023-02-21 | 日商組合化學工業股份有限公司 | Pesticide formulation composition using trichoderma, method of production thereof, and application method |
| CN105831175A (en) * | 2016-04-29 | 2016-08-10 | 鞍山禾瑞生物科技有限公司 | Liquid compound biological multi-control fungicide and preparation method and application thereof |
| CN105831175B (en) * | 2016-04-29 | 2018-03-20 | 鞍山禾瑞生物科技有限公司 | A kind of liquid compound bio controls bactericide and preparation method and application more |
| CN106212122A (en) * | 2016-07-26 | 2016-12-14 | 宾阳县华信种植专业合作社 | A kind of method preventing and treating Fructus Gardeniae grandiflorae daughter root maize ear rot |
| CN112940946A (en) * | 2021-01-27 | 2021-06-11 | 贵州大学 | Method for preparing paecilomyces lilacinus from marigold straws to prevent and control tobacco root-knot nematodes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102792973B (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102792973B (en) | Compound bactericide and preparation method thereof | |
| CN103931671A (en) | Plant seed soaking and dressing agent | |
| CN103503924B (en) | Preparation method and applications of fusarium oxysporum inoculant | |
| TW201218956A (en) | Compositions containing anthraquinone derivatives as growth promoters and antifungal agents | |
| CN101342374A (en) | Apparatus of ozone for preventing and controlling plant diseases and pests, and disinfection method thereof | |
| CN102838389B (en) | Fertilizer-and-drug double-effect bio-organic fertilizer for preventing and treating crop soil-borne diseases and manufacturing method of bio-organic fertilizer | |
| CN104904761A (en) | Biopesticide and usage method thereof | |
| CN101695578A (en) | Nuisanceless soil disinfection method | |
| CN101606536B (en) | Wood vinegar soil disinfectant and preparation method thereof | |
| CN101491244A (en) | Application technique of compound of dimethyl disulfide and chloreopicrin as soil fumigant | |
| US20190327961A1 (en) | Stable solid composition based on an aromatic compound and uses thereof | |
| CN105794801A (en) | Antifungal application of essential oil composition to mildew fungi | |
| CN104824040A (en) | Biological insecticidal rooting dual protective agent and preparation method and application method thereof | |
| KR20120068674A (en) | Streptomyces griseus big105 for controlling plant diseases and uses thereof | |
| KR102068090B1 (en) | Commposition for accelerating growth of plants using bacillus amyloliquefaciens and use thereof | |
| CN104430644A (en) | Biopesticide without pesticide residue | |
| CN105941485A (en) | Efficient garden herbicide | |
| CN104351210B (en) | Preparation method of cottonseed bactericidal hybridization solution containing sodium dichloro cyanurate and hymexazol | |
| CN107136073A (en) | One kind contains Physcion and methoxy acrylic bactericide composition | |
| CN106070357A (en) | A kind of novel wheat field compound disinfectant and application thereof | |
| CN107114620B (en) | Predatory mite culture base material and using method thereof | |
| CN110200026A (en) | A kind of plant and microorganism composite insecticide and preparation method thereof | |
| KR101815877B1 (en) | Mixed solution for foliar fertilization | |
| KR102048071B1 (en) | foam control agent for prevention of rice damages by diseases and insects | |
| CN107156136A (en) | The fluorine bacterium azoles of alkene containing benzo and the composition pesticide of benzamides bactericide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170317 Address after: Room A-2859, room 2, building 3, No. 30, Xing Xing street, Shijingshan District, Beijing Patentee after: Beijing China Environmental Protection Technology Co.,Ltd. Address before: 034000 Shanxi city of Xinzhou province Dingxiang County Yang Fang Chi Village Industrial Park Patentee before: Cui Ruizhen Patentee before: Gao Xiuhong Patentee before: Dong Hailong |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 Termination date: 20210728 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |