KR102048071B1 - foam control agent for prevention of rice damages by diseases and insects - Google Patents

Abstract

The present invention relates to a foam control agent for preventing rice pests, more specifically, to a foam control agent for preventing rice pests, which is easy to fertilize by spraying on rice paddy fields and foaming in water, and has an excellent pest control effect such as bacterial blight, rice blast, a leafhopper, and the like. The foam control agent for preventing rice pests of the present invention comprises: 50 to 100 parts by weight of citric acid; 2 to 10 parts by weight of nim oil; and 0.1 to 2 parts by weight of Bacillus subtilis fungi with respect to 100 parts by weight of sodium bicarbonate.

Description

Foam control agent for prevention of rice damages by diseases and insects}

The present invention relates to a foam control agent for preventing rice pests, and more particularly, it is easy to fertilize by spraying on paddy fields and foaming in water, and the foam control agent for preventing rice pests having excellent pest control effects such as rice leaf blight, rice blast bottle, rice plant, etc. It is about.

Most crops are pesticides caused by germs or pests, and the yield is reduced or the quality is poor.

In other words, all useful plants including crops, vegetables, fruit trees, flowers, trees, etc., are killed and decayed by germs, resulting in reduced yields or cooling and absorption by poor quality pests and insects. Due to the loss, special attention is paid to pests.

The main pests of rice are rice leaf blight and rice blast. And the main pest of rice is the rice hopper. Many methods have been attempted to prevent and treat these pests to maintain crop yields and to obtain high quality crops. Prevention, treatment and quarantine of viruses against crops are important challenges in agriculture.

Various pesticides have been developed to prevent pests. However, most of the formulation of the developed product is a liquid phase, resulting in increased logistics costs and shorter shelf life is a problem. In addition, when using a liquid or powdered product as a hydrating agent, facility horticultural crops are treated with irrigation using a pipe in the soil, resulting in clogging of the irrigation holes, which is disadvantageous in that it is inconvenient when used for cultivating the open field. In addition, the worker is often exposed to dust, steam, odor generated when opening the pesticide container, and mist containing the active ingredient of the pesticide during spraying operation, often causes serious sequelae.

In addition, as a method of spraying pesticides such as powder and granules, in this case, in addition to the above-mentioned problems, a large amount of dust or the like may be generated by the wind or the like during spraying of pesticides, and the worker may be exposed as it is, thus causing a fatal effect on health.

In particular, the methods of spraying pesticides are sprayed by hand or sprayed by hand over a large area. In the case of manual and mechanical sprayers, the average weight of sprayers including pesticides is about 20 to 40 kg. It is heavy about 3 ~ 5kg per bag and requires excessive labor, and it takes a long time to complete the processing, which is a great burden for agricultural workers.

As a method for reducing the labor force in the Republic of Korea Patent No. 10-0768273 discloses a method for producing and using a viable foaming pesticide composition.

The foamable pesticide composition can be easily completed by spraying the pesticide active ingredient in a large area by the foaming power of carbon dioxide generated as a result of a chemical reaction with water, if simply thrown directly from the rice paddy without spraying by hand directly into the paddy field There is an advantage.

However, the foamable pesticide composition has a problem in that a synthetic chemical is used as the active ingredient of the pesticide, and a chemical surfactant is used for the purpose of increasing the diffusibility of the active ingredient of the pesticide.

Republic of Korea Patent No. 10-0768273: Preparation and use method of viable foamable pesticide composition

The present invention was created to improve the above problems, it is possible to improve the diffusibility by spraying on paddy field to form an oil film on the water surface when foaming in water and excellent pest control effect such as rice leaf blight, rice blast, rice plant And it is an object to provide a foam control agent for preventing rice pests easy to fertilize.

Foam control agent for preventing rice pests of the present invention for achieving the above object 50 to 100 parts by weight of citric acid, 2 to 10 parts by weight of nim oil, 0.1 to 2 Bacillus subtilis fungus with respect to 100 parts by weight of sodium hydrogencarbonate It contains parts by weight.

And it further contains 1 to 5 parts by weight of gondal extract.

In addition, the control agent controls rice blight or rice blast.

In addition, the control agent controls the rice plant.

As described above, the present invention is formulated in the form of a tablet and can be sprayed in a manner of throwing into the paddy field, so that storage safety is high, easy to use, labor can be saved, and fertilization is very easy.

In addition, the present invention is excellent in diffusibility because the nyum oil, which is an active ingredient for controlling pests by foaming in water, is diffused in a manner of forming an oil film on the surface of the water, and has excellent pest control effects such as rice leaf blight, rice blast, and rice hopper.

In addition, the present invention is harmless to crops and the human body by using a substance derived from nature such as Nim oil as the active ingredient of pest control.

Hereinafter, a foam control agent for preventing rice pests according to a preferred embodiment of the present invention will be described in detail.

The present invention contains, for example, sodium bicarbonate, organic acid, nim oil, Bacillus subtilis bacteria.

Sodium bicarbonate, along with citric acid, foams in water to produce carbon dioxide. In addition to sodium hydrogen carbonate, any one of calcium carbonate, potassium carbonate and potassium hydrogen carbonate can be used.

Citric acid (citric acid), tartaric acid (tartaric acid), oxalic acid (oxalic acid), maleic acid (maleic acid), succinic acid (succinic acid), adipic acid (adipic acid) may be used as the organic acid.

Preferably citric acid is used as the organic acid. Citric acid has excellent antioxidant activity and acts as a preservative and stabilizer.

Neem oil is an oil extracted from the seeds of the Azadirachta indica . Azadirachtin, a component of Nim oil, is known to have an insecticidal effect.

In the present invention, since the neem oil is used as an active ingredient of the preservative, the neem oil floats to the surface when foamed in water and is diffused by forming a thin oil film on the water surface. Therefore, the diffusibility of nim oil can be improved. As such, an oil film of neem oil is formed on the surface of the water, which is effective for controlling pests such as pests such as rice planthopper.

Bacillus subtilis fungus may be obtained in powder form by lyophilizing Bacillus subtilis culture.

The Bacillus microorganism is the second most widely researched and commercialized bacterium after Pseudomonas, which produces a variety of biologically active secondary metabolites, and is characterized by forming endospores that are stable to heat and resistant to harsh environments. . Bacillus subtilis ( Bacillus subtilis ) is used as the strain.

Bacillus subtilis spawn is inoculated in LB (Luria Bertani) medium and spawned at 30-35 ° C. for 24 to 48 hours, followed by inoculation into the main culture medium. The culture medium may be prepared by mixing glucose, yeast extract, peptone, potassium phosphate, sodium chloride, sodium carbonate and magnesium sulfate in sterile distilled water. For example, 6 g of glucose per liter of medium, 5 g of yeast extract, 10 g of peptone, 2.5 g of potassium phosphate, 1.5 g of sodium chloride, 0.5 g of sodium carbonate, and 1 g of magnesium sulfate.

Bacillus subtilus is incubated for 1 to 3 days at 25 to 35 ℃ using the above-described culture medium. Through the method of incubating for 1 to 3 days at 25 to 35 ℃ in the main culture medium can greatly increase the content of iturin (iturin) and sulfactin (surfactin) in the culture. Iturin and sulfactin are produced as a metabolite of Bacillus subtilis and have excellent antibacterial properties.

The culture solution obtained by culturing in the main culture medium may be frozen at −40 ° C. using a vacuum freeze dryer, and then heated to 30 ° C. to dry.

Sodium bicarbonate, citric acid, nim oil, and Bacillus subtilis fungi are mixed evenly. For example, 50 to 100 parts by weight of citric acid, 2 to 10 parts by weight of nim oil, and 0.1 to 2 parts by weight of Bacillus subtilis fungus may be mixed with 100 parts by weight of sodium bicarbonate.

Sodium bicarbonate, citric acid, nim oil, and Bacillus subtilis fungi are mixed evenly and then pressurized using a tableting machine to form a shape and size to produce a foaming control agent.

Foam control agents can be formed into tablets such as pellets, spheres, and discs. For example, a foam control agent may be made into a disc of 5 to 50 g in weight.

On the other hand, the present invention may further contain a gondal extract. For example, the present invention mixes 50 to 100 parts by weight of citric acid, 2 to 10 parts by weight of nim oil, 0.1 to 2 parts by weight of Bacillus subtilis fungus, and 1 to 5 parts by weight of gondal extract based on 100 parts by weight of sodium hydrogen carbonate. Then, by using a tableting machine molded into a predetermined shape and size to prepare a foam control agent.

Gondalbi extract increases the antioxidant and antimicrobial activity of the control agent.

Gondalbi (Ligularia stenocephala ) is a perennial herb of the dicotyledon plant Lantern Asteraceae, distributed in Korea, Japan, Taiwan, and China, and grows in deep mountain marshes. Gondalbi mainly consumes young leaves as herbs, and herbal medicines use outposts or roots as medicine for bowel, soothing and gynecological diseases. Gondalbi is used in various ways such as Ssam, Namul, Pickles and Kimchi as well as Bear. The reported substances isolated from gondalbi are 1β, 6α-dihydroxy-5 (15) -eudesmene, phthalic acid di-Bu ester, N-phenyl-2-naphthylamine, neophytadiene, vanillin, triterpenoid derivatives, 4-hydroxyacetophenone, chlorogenic acid, caffeic acid.

Gondalbi extract can be extracted in a variety of ways by adding an extraction solvent. For example, hot water extraction, cold or hot needle extraction may be performed by adding an extraction solvent to the gondal ratio. In this case, the extraction solvent may be added to the gondal ratio by adding 2 to 20 times by weight, followed by extraction at 10 to 150 ° C. for 1 to 24 hours.

Here, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, Butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. And the mixture may be a mixture of water and lower alcohol, a mixture of water and polyhydric alcohol, a mixture of lower alcohol and polyhydric alcohol, or a mixture of water and lower alcohol and polyhydric alcohol.

And it is a matter of course that the extract can be obtained in powder form by extracting the extract using the extraction solvent by filtration, concentrated under reduced pressure or freeze-drying, spray drying.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited by the following examples.

(Example 1)

75 parts by weight of citric acid, 6 parts by weight of nim oil, and 0.5 parts by weight of Bacillus subtilis fungus were mixed with 100 parts by weight of sodium bicarbonate, and then molded into a tablet using a tableting machine to prepare a foamed control agent. The weight of the foam control agent was about 30 g.

Bacillus subtilis bacteria were prepared as follows.

Bacillus subtilis ( Bacillus subtilis ) was inoculated in 3L LB medium and spawned for 36 hours at 32 ℃, 180rpm conditions. After spawn culture, 30 in main culture medium (glucose 6g / l, yeast extract 5g / l, peptone 10g / l, potassium phosphate 2.5g / l, sodium chloride 1.5g / l, sodium carbonate 0.5g / l, magnesium sulfate 1g / l) The culture solution was obtained by culturing at 2 ° C. for 2 days and then vacuum-freezing using a vacuum freezer to obtain a powdered bacterium.

(Example 2)

To prepare a foam control agent in the same manner as in Example 1, 75 parts by weight of citric acid, 6 parts by weight of nim oil, 0.5 parts by weight of Bacillus subtilis bacteria, 1 to 5 parts by weight of gondal extract The mixture was used.

Ethanol was added to the gondal ratio as a solvent by weight 10 times, extracted at 90 ℃ for 8 hours, concentrated under reduced pressure and lyophilized to use as a powder.

< On rice fever  For investigation

Pyricularia causing schizophrenia Oryzae strains were inoculated in PDA medium and incubated for one week in a 25 ° C. incubator.

Pyricularia a week after spraying 10 per 100 pyeong of the foam control agent of Example 1 to the rice planted water in rice plants grown for 3 months in a greenhouse Oryzae spores were prepared at 1 × 10 ⁵ spores / ml and sprayed to ensure sufficient wetness in rice. In order to prevent the leaves from drying immediately after spraying, the inoculated rice was covered with vinyl for 2 days to increase the incidence.

Two weeks after the pathogen inoculation, the lesion area rate was measured in the plaques that appeared on the leaves of rice. The mean diseased area ratio of stems was the total disease area ratio per diseased abandonment and the number of irradiated birds. The control value was expressed as (1- (lesion area ratio of treatment / no area of treatment)) * 100.

The area ratio and the control value of the treated area sprayed with the foamed control agent and the untreated tool sprayed with the foamed control agent are shown in Table 1 below.

division Lesion area rate (%) Control price (%) No treatment 69.4% ± 2.74 - Treatment 30.2% ± 2.31 56.48

Referring to the results of Table 1, the treated area was found to reduce the lesion area by 56% compared to the untreated. Therefore, it was confirmed that the foam control agent of the present invention has a control effect against rice blast.

<Rice Investigation into control of leaf blight >

In-flight experiments were conducted to determine the effectiveness of the control against the white leaf blight caused by rice cultivation. Xanthomonas oryzae causes white leaf blight in rice pv . Oryzae K3 race was incubated in NB medium, culture conditions were incubated for more than 48 hours in a 28 ℃ shaking incubator (200rpm).

Xanthomonas a week after spraying 10 foamy control agents of Example 1 to 100 pyeong of rice planted rice plant grown in a greenhouse for 3 months oryzae pv . Oryzae K3 race was inoculated at OD (600 nm) = 1.0 with sterile water.

Effervescent control agents were treated once, and one week after treatment, the inoculated cultured pathogens were inoculated with 15 leaves per week through scissor inoculation and the incidence and incidence of disease for two weeks. The disease occurrence was started by cutting with scissors, and the ratio of the total area to the diseased area that progressed to the lower section was statistically analyzed. The popular rice varieties were used. The control value is expressed as (1- (translational distance of the treatment / no-translational distance of the treatment)) * 100.

The translational distances and control values of the treated group sprayed with the foamed control agent and the untreated sprayed group without the foamed control agent are shown in Table 2 below.

division Bottle progression (cm) Control Price (%) No treatment 18.21cm ± 1.015 - Treatment 9.32 cm ± 0.23 48.82

Referring to the results of Table 2, the treatment-free path was 18.21 cm in the treatment group, the treatment path was 9.32 cm in the treatment group, the treatment group was less translational distance than the control group, the control value was 48.82%. Therefore, it was confirmed that the foam control agent of the present invention has a control effect against rice leaf blight.

< Investigation into control of rice plant >

In order to conduct experiments to control rice planting, rice seedlings and pots were planted in cages (60cm * 60cm), and the number and density of the inside of the cage were checked before treatment. And after spraying the foam control agent of Example 1 to the water inside the pot, the insecticidal rate and control value after 1 day, 3 days, 5 days were investigated and the results are shown in Table 3 below.

division Pre-treatment Insecticide rate (%) 1 day 3 days 5 days No treatment 33.3 - - - Treatment 32.3 48.7 57.3 67.1 Density before treatment Control price (%) 1 day 3 days 5 days No treatment 52.3 - - - Treatment 55.3 44.5 46.3 48.3

Referring to the results of Table 3, the treated group treated with the foamed control agent exhibited a 48.7% insecticide rate per day and 67.1% after 5 days compared to the untreated group treated with the foamed control agent. When the control price was confirmed, it was 44.5% after 1 day and 48.3% after 5 days. This confirmed that the foam control agent of the present invention has a control effect against rice bran.

Through the above experimental results, we could see the control effect on the rice and white leaf blight that occur in rice, and the effect of controlling rice planthopper through nim oil contained in the foam control agent was also confirmed.

On the other hand, in order to compare the control effect of Example 1 and Example 2 was investigated the control value for rice fever and rice leaf blight in the same manner as the above experimental method.

As a result, the treatment group treated with the foaming agent of Example 2 was found to improve about 12% for rice fever and about 16% for rice blight compared to the treatment group treated with the foaming agent of Example 1. .

As described above, the present invention has been described with reference to one embodiment, which is merely exemplary, and those skilled in the art will understand that various modifications and equivalent embodiments are possible therefrom. Therefore, the true scope of protection of the present invention should be defined only by the appended claims.

Claims (4)

50 to 100 parts by weight of citric acid, 2 to 10 parts by weight of nim oil, 0.1 to 2 parts by weight of Bacillus subtilis fungus, and 1 to 5 parts by weight of gondal extract, based on 100 parts by weight of sodium bicarbonate,
Janto Pseudomonas duck chair (Xanthomonas oryzae pv. Oryzae) Rice claim huinip blight or pyrido Kula Ria duck Here the foamable controlling rice pests, characterized in that for preventing having antifungal activity against rice blast by (Pyricularia oryzae) by. delete delete [Claim 2] The foam control agent for preventing rice pests according to claim 1, wherein the control agent further has a control activity against rice plant bulbs.