CN102792825A - Preservation method for rhizoma bletillae seeds - Google Patents
Preservation method for rhizoma bletillae seeds Download PDFInfo
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- CN102792825A CN102792825A CN2012102967689A CN201210296768A CN102792825A CN 102792825 A CN102792825 A CN 102792825A CN 2012102967689 A CN2012102967689 A CN 2012102967689A CN 201210296768 A CN201210296768 A CN 201210296768A CN 102792825 A CN102792825 A CN 102792825A
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Abstract
The invention discloses a preservation method for rhizoma bletillae seeds, comprising the following steps of: collecting uncracked rhizoma bletillae capsules, immersing the collected rhizoma bletillae capsules for 30s on a super clean workbench by using 75wt% of alcohol, and soaking up the alcohol on the surfaces of the rhizoma bletillae capsules by using sterilization filter paper; then, cleaning the rhizoma bletillae capsules 3-5 times by using sterile water, and soaking up the liquid on the surfaces of the rhizoma bletillae capsules by using the sterilization filter paper every time after the cleaning; placing the cleaned rhizoma bletillae capsules on the super clean workbench, baking on the outer flame of naked flame of an alcohol lamp at the temperature of 400-500 DEG C for 3-6s; and placing the baked rhizoma bletillae capsules in a 4-DEG C refrigerator for preservation. According to the preservation method, the collected rhizoma bletillae seeds are preserved at low temperature after being treated, and the rhizoma bletillae seeds which are preserved for over one year still have a seed germination rate close to that of fresh matured seeds collected in the same year after being seeded. An effective approach is provided for the preservation and the research of rhizoma bletillae seed resources.
Description
Technical field
The present invention relates to field of agricultural science and technology, especially a kind of store method of bletilla seed.
Background technology
Bletilla
Bletilla striataBe the perennial perennial root herbelet of the orchid family, be used as medicine that the bletilla stem tuber contains bletilla colloid (mucilaginous substance), starch, volatile oil, glucose etc. with dry tuber.Bletilla bitter, sweet, puckery; Cold nature; Return lung, liver, stomach warp; Has astringing to arrest bleeding, the function of detumescence and promoting granulation.Be mainly used in treatment hemoptysis, haematemesis, traumatism and bleeding, sore swollen toxin, chapped skin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage etc.Except that above clinical medicinal, bletilla glue belongs to the tubers natural macromolecule amylose, can replace west yellow work rubber powder and gummi arabicum pulveratum to be used for food and chemical field as emulsifier, suspending agent.Because high economic worth; Market demand increases year by year, and going to wreck property of wild resource is excavated, and it is in imminent danger that wild resource has been tending towards; The plant that is put into the rare and endangered of country is protected; But also record in international trade pact (CITES) appendix two, receive The International Protection (Xu Zhongzhi etc., 2011).Bletilla nature ripening rate is higher; Seed nature germination rate is extremely low; Utilize seed to carry out aseptic seeding, it is that effective way is protected and developed to bletilla that tissue-culturing rapid propagation is grown seedlings, along with the bletilla wild resource is rare; It is very rare that the bletilla capsule also becomes, and needs to strengthen the bletilla seed resource is effectively preserved.But, lack a kind of method that can effectively preserve the bletilla seed for a long time now.
Summary of the invention
The objective of the invention is: a kind of store method of bletilla seed is provided, the preservation bletilla seed that it can be permanently effective, and can guarantee that the germination rate of sowing again after it is preserved is consistent with fresh mature seed, to overcome the deficiency of prior art.
The present invention is achieved in that the store method of bletilla seed, gathers uncracked bletilla capsule, and the bletilla capsule that collects after the use mass percent is 75% alcohol-pickled 30s on the superclean bench, is blotted the alcohol on bletilla capsule surface with sterilization filter paper; With sterile water the bletilla capsule is washed 3 ~ 5 times again, all blot after each washing with the liquid of aseptic filter paper with bletilla capsule surface; The bletilla capsule that will pass through washing places on the superclean bench, toasts with alcolhol burner naked light flame envelope, and the temperature of baking is 400～500 ℃, and stoving time is 3～6 seconds, plays oven dry and sterilization functions; To be positioned over through the bletilla capsule of overbaking in 4 ℃ the refrigerator and preserve.
Will be through the bletilla capsule of overbaking earlier with the bale of cotton on, refill into tying in the M.G. pure sulphite paper, make it play dampproof effect, be positioned in 4 ℃ the refrigerator and preserve.
During 8～October, gather uncracked bletilla capsule.This moment, seed was in the maturing stage, if acquisition time is late excessively, then because the bletilla capsule ftractures, seed is scattered and influences the quality of collection.
Owing to adopted technique scheme; Compared with prior art; The present invention handles the storage of back implementation low temperature to gathering the bletilla capsule; The bletilla seed that stores more than 1 year still has the sowing germination rate that is close with the fresh mature seed of gathering then after planting, the present invention provides a valid approach for the preservation and the research of bletilla seed resource.The present invention is simple, and is with low cost, and result of use is good.
Embodiment
Embodiments of the invention: the store method of bletilla seed, in Guizhou Province, academy of agricultural sciences, Guizhou Province, Guiyang modern Chinese herbal medicine material research institute traditional Chinese medicine germ plasm resource garden bletilla experimental field, 26 ° of 30 ' 9.3 " N, 106 ° 39 ' 43.3 " E, height above sea level 1122m; (orchid comprises that bletilla all is rare or endangered species to gather 10 uncracked bletilla capsules on September 12nd, 2010; Solid limited amount; Can only pluck once), Guizhou Province's agricultural biotechnologies key lab group training chamber adopts technical scheme of the present invention to handle in the academy of agricultural sciences, Guizhou Province, and concrete processing procedure is; The bletilla capsule that collects after the use mass percent is 75% alcohol-pickled 30s on the superclean bench, is blotted the alcohol on bletilla capsule surface with sterilization filter paper; With sterile water the bletilla capsule is washed 5 times again, all blot after each washing with the liquid of aseptic filter paper with bletilla capsule surface; The bletilla capsule that will pass through washing places on the superclean bench, toasts with alcolhol burner naked light flame envelope, and the temperature of baking is 400～500 ℃, and stoving time is 3～6 seconds, plays oven dry and sterilization functions; Will through the bletilla capsule of overbaking earlier with the bale of cotton on, refill into tying in the M.G. pure sulphite paper, be positioned in 4 ℃ the refrigerator and preserve.
Take out an above-mentioned bletilla capsule on September 20th, 2011, Guizhou Province's agricultural biotechnologies key lab group training chamber aseptic seeding carries out tissue-culturing rapid propagation and cultivates the bletilla seedling in the academy of agricultural sciences, Guizhou Province.Carried out aseptic seeding the same period, carries out comparative trial from experimental field gathering 1 of fresh capsule then in 1 day in advance.Bletilla seed germination medium: 1/2MS+NAA0.5mg/L+ agar powder 0.7%+ white sugar 3%, pH5.8-6.0 uses the tissue culture bottle packing behind the high-temperature boiling, every bottle of 40ml, autoclave sterilization 23min cools off subsequent use.On superclean bench; Earlier with 75% cotton ball soaked in alcohol sassafras examination sterilization bletilla capsule surface; Then capsule is dipped in 95% alcohol ignition sterilization 4 times; Draw with aseptic scalpel and to break in the seed coat, tiny seed broadcasting on prepared culture medium, is positioned over (25 ± 2) ℃, illumination 12h/d, light intensity 1500-2000Lx condition of culture is cultivated down.After 10 days, seed begins to sprout, and sprouts after 30 days to emerge comparatively neatly, observes its sprouting situation, finds that the bletilla seed of preserving carries out aseptic seeding, and is consistent with the fresh mature seed aseptic seeding germination rate performance of gathering then.
Claims (3)
1. the store method of a bletilla seed is characterized in that: gather uncracked bletilla capsule, the bletilla capsule that collects after the use mass percent is 75% alcohol-pickled 30s on the superclean bench, is blotted the alcohol on bletilla capsule surface with sterilization filter paper; With sterile water the bletilla capsule is washed 3 ~ 5 times again, all blot after each washing with the liquid of aseptic filter paper with bletilla capsule surface; The bletilla capsule that will pass through washing places on the superclean bench, toasts with alcolhol burner naked light flame envelope, and the temperature of baking is 400～500 ℃, and stoving time is 3～6 seconds; To be positioned over through the bletilla capsule of overbaking in 4 ℃ the refrigerator and preserve.
2. the store method of bletilla seed according to claim 1 is characterized in that: will be through the bletilla capsule of overbaking earlier with the bale of cotton on, refill into tying in the M.G. pure sulphite paper, be positioned in 4 ℃ the refrigerator and preserve.
3. the store method of bletilla seed according to claim 1 is characterized in that: during 8～October, gather uncracked bletilla capsule.
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| CN201210296768.9A CN102792825B (en) | 2012-08-21 | 2012-08-21 | Preservation method for rhizoma bletillae seeds |
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| CN201210296768.9A CN102792825B (en) | 2012-08-21 | 2012-08-21 | Preservation method for rhizoma bletillae seeds |
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| CN102792825A true CN102792825A (en) | 2012-11-28 |
| CN102792825B CN102792825B (en) | 2015-04-01 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103999643A (en) * | 2014-06-04 | 2014-08-27 | 湄潭县高岩玄参中药材专业合作社 | Preservation method of medicinal rhubarb seeds |
| CN104012523A (en) * | 2014-05-28 | 2014-09-03 | 中国林业科学研究院亚热带林业研究所 | Embedding vitrification ultralow-temperature storage method for bletilla striata seeds |
| CN104718841A (en) * | 2015-02-26 | 2015-06-24 | 李泽诚 | Treatment method of rhizoma bletillae seeds |
| CN105027886A (en) * | 2015-06-25 | 2015-11-11 | 洪江市金土地生态农业有限责任公司 | Rhizoma bletillae seed direct seeding reproduction method |
| CN105941392A (en) * | 2016-05-03 | 2016-09-21 | 湖南农业大学 | Bletilla striata capsule preserving method suitable for tissue culture and rapid propagation |
| CN106941890A (en) * | 2017-03-23 | 2017-07-14 | 杨军 | A kind of storage method of willow seed |
| CN107549823A (en) * | 2017-09-08 | 2018-01-09 | 四川桑果白兰地农业开发有限公司 | A kind of method for sorosis processing |
| CN107926936A (en) * | 2017-11-30 | 2018-04-20 | 张家界本草科技有限公司 | A kind of bletilla seed cryopreservation method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012523A (en) * | 2014-05-28 | 2014-09-03 | 中国林业科学研究院亚热带林业研究所 | Embedding vitrification ultralow-temperature storage method for bletilla striata seeds |
| CN103999643A (en) * | 2014-06-04 | 2014-08-27 | 湄潭县高岩玄参中药材专业合作社 | Preservation method of medicinal rhubarb seeds |
| CN103999643B (en) * | 2014-06-04 | 2016-06-08 | 湄潭县高岩玄参中药材专业合作社 | The store method of Rheum officinale seed |
| CN104718841A (en) * | 2015-02-26 | 2015-06-24 | 李泽诚 | Treatment method of rhizoma bletillae seeds |
| CN105027886A (en) * | 2015-06-25 | 2015-11-11 | 洪江市金土地生态农业有限责任公司 | Rhizoma bletillae seed direct seeding reproduction method |
| CN105941392A (en) * | 2016-05-03 | 2016-09-21 | 湖南农业大学 | Bletilla striata capsule preserving method suitable for tissue culture and rapid propagation |
| CN105941392B (en) * | 2016-05-03 | 2018-10-09 | 湖南农业大学 | A kind of bletilla striata capsule store method suitable for tissue-culturing rapid propagation |
| CN106941890A (en) * | 2017-03-23 | 2017-07-14 | 杨军 | A kind of storage method of willow seed |
| CN107549823A (en) * | 2017-09-08 | 2018-01-09 | 四川桑果白兰地农业开发有限公司 | A kind of method for sorosis processing |
| CN107926936A (en) * | 2017-11-30 | 2018-04-20 | 张家界本草科技有限公司 | A kind of bletilla seed cryopreservation method |
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