CN102792825B - Preservation method for rhizoma bletillae seeds - Google Patents
Preservation method for rhizoma bletillae seeds Download PDFInfo
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- CN102792825B CN102792825B CN201210296768.9A CN201210296768A CN102792825B CN 102792825 B CN102792825 B CN 102792825B CN 201210296768 A CN201210296768 A CN 201210296768A CN 102792825 B CN102792825 B CN 102792825B
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Abstract
The invention discloses a preservation method for rhizoma bletillae seeds, comprising the following steps of: collecting uncracked rhizoma bletillae capsules, immersing the collected rhizoma bletillae capsules for 30s on a super clean workbench by using 75wt% of alcohol, and soaking up the alcohol on the surfaces of the rhizoma bletillae capsules by using sterilization filter paper; then, cleaning the rhizoma bletillae capsules 3-5 times by using sterile water, and soaking up the liquid on the surfaces of the rhizoma bletillae capsules by using the sterilization filter paper every time after the cleaning; placing the cleaned rhizoma bletillae capsules on the super clean workbench, baking on the outer flame of naked flame of an alcohol lamp at the temperature of 400-500 DEG C for 3-6s; and placing the baked rhizoma bletillae capsules in a 4-DEG C refrigerator for preservation. According to the preservation method, the collected rhizoma bletillae seeds are preserved at low temperature after being treated, and the rhizoma bletillae seeds which are preserved for over one year still have a seed germination rate close to that of fresh matured seeds collected in the same year after being seeded. An effective approach is provided for the preservation and the research of rhizoma bletillae seed resources.
Description
Technical field
The present invention relates to field of agricultural science and technology, especially a kind of store method of bletilla seed.
Background technology
Bletilla
bletilla striatabe the orchid family perennial root herbelet, be used as medicine with dry tuber, bletilla stem tuber is containing bletilla colloid (mucilaginous substance), starch, volatile oil, glucose etc.Bletilla bitter, sweet, puckery; Cold nature; Return lung, liver, stomach warp; There is astringing to arrest bleeding, the function of detumescence and promoting granulation.Be mainly used in treatment hemoptysis, haematemesis, traumatism and bleeding, sore swollen toxin, chapped skin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage etc.Except above clinical medicinal except, gum bletilla belongs to tubers natural macromolecule amylose, can replace west yellow work rubber powder and gummi arabicum pulveratum as emulsifier, suspending agent for food and chemical field.Due to high economic worth; market demand increases year by year; going to wreck property of wild resource is excavated; wild resource has been tending towards in imminent danger; the plant being put into the Precious, Rare, Endangered of country is protected; but also record in international trade pact (CITES) annex two, be subject to the International Protection (Xu Zhongzhi etc., 2011).Bletilla Natural seed setting rate is higher; seed nature germination rate is extremely low; seed is utilized to carry out aseptic seeding; tissue-culturing rapid propagation nursery carries out protection and developed application effective way to bletilla; along with bletilla wild resource is rare; bletilla capsule also becomes very rare, need strengthen effectively preserving bletilla seed resource.But, lack now a kind of method effectively can preserving bletilla seed for a long time.
Summary of the invention
The object of the invention is: the store method that a kind of bletilla seed is provided, the preservation bletilla seed that it can be permanently effective, and can ensure that the germination rate sowed again after it is preserved is consistent with fresh mature seed, to overcome the deficiencies in the prior art.
The present invention is achieved in that the store method of bletilla seed, gathers uncracked bletilla capsule, is after the alcohol-pickled 30s of 75%, blots the alcohol on bletilla capsule surface with sterilizing filter paper by the bletilla capsule collected on superclean bench by mass percent; With sterile water, bletilla capsule is washed 3 ~ 5 times again, all blot with the liquid of aseptic filter paper by bletilla capsule surface after each washing; Be placed on superclean bench by the bletilla capsule through washing, toast with alcolhol burner naked light flame envelope, the temperature of baking is 400 ~ 500 DEG C, and baking time is 3 ~ 6 seconds, plays oven dry and sterilization functions; Bletilla capsule through overbaking is positioned in the refrigerator of 4 DEG C and preserves.
By the bletilla capsule through overbaking first with on the bale of cotton, refill tying in M.G. pure sulphite paper, make it play moistureproof effect, be positioned in the refrigerator of 4 DEG C and preserve.
Uncracked bletilla capsule is gathered during 8 ~ October.Now seed is in the maturing stage, if acquisition time is excessively late, then due to bletilla capsule cracking, seed is scattered and affects the quality of collection.
Owing to have employed technique scheme, compared with prior art, the present invention processes rear implementation low temperature to collection bletilla capsule and stores, the bletilla seed storing more than 1 year after planting still has the sowing germination rate be close with the fresh mature seed of gathering then, and the present invention is that the preservation of bletilla seed resource and research provide an effective approach.The present invention is simple, with low cost, and result of use is good.
Embodiment
Embodiments of the invention: the store method of bletilla seed, at modern Chinese herbal medicine material research institute of Guizhou Academy of Agricultural Sciences Guizhou Province, Guiyang traditional Chinese medicine Germplasm Resources bletilla experimental field, 26 ° 30 ' 9.3 " N, 106 ° 39 ' 43.3 " E, height above sea level 1122m; (it is all rare or endangered species that orchid comprises bletilla to gather 10 uncracked bletilla capsules on September 12nd, 2010, fruit amount is limited, can only pluck once), technical scheme of the present invention is adopted to process in agricultural biotechnologies key lab of Guizhou Academy of Agricultural Sciences Guizhou Province group training room, concrete processing procedure is, be after the alcohol-pickled 30s of 75% by mass percent on superclean bench by the bletilla capsule collected, blot the alcohol on bletilla capsule surface with sterilizing filter paper; With sterile water, 5 times are washed to bletilla capsule again, all blot with the liquid of aseptic filter paper by bletilla capsule surface after each washing; Be placed on superclean bench by the bletilla capsule through washing, toast with alcolhol burner naked light flame envelope, the temperature of baking is 400 ~ 500 DEG C, and baking time is 3 ~ 6 seconds, plays oven dry and sterilization functions; By bletilla capsule through overbaking first with on the bale of cotton, refill tying in M.G. pure sulphite paper, be positioned in the refrigerator of 4 DEG C and preserve.
At the bletilla capsule that taking-up on September 20th, 2,011 one is above-mentioned, at agricultural biotechnologies key lab of Guizhou Academy of Agricultural Sciences Guizhou Province group training room aseptic seeding, carry out tissue-culturing rapid propagation and cultivate bletilla seedling.Within 1 day in advance, from fresh capsule 1 of experimental field gathering then, the same period carries out aseptic seeding, carries out comparative trial.Bletilla seed germination medium: 1/2MS+NAA0.5mg/L+ agar powder 0.7%+ white sugar 3%, pH5.8-6.0, uses tissue culture bottle packing, every bottle of 40ml, autoclave sterilization 23min, cools for subsequent use after high-temperature boiling.On superclean bench, first with 75% cotton ball soaked in alcohol sassafras examination sterilization bletilla capsule surface, then capsule is dipped in 95% alcohol ignition sterilizing 4 times, seed coat is scratched with sterile scalpel, by tiny seed broadcasting on the medium prepared, cultivate under being positioned over (25 ± 2) DEG C, illumination 12h/d, light intensity 1500-2000Lx condition of culture.After 10 days, seed starts to sprout, and within 30 days, sprouts afterwards and emerges comparatively neatly, observe its sprouting situation, find that the bletilla seed preserved carries out aseptic seeding, show consistent with the fresh mature aseptically sowing seeds germination rate of gathering then.
Claims (3)
1. a store method for bletilla seed, is characterized in that: gather uncracked bletilla capsule, is after the alcohol-pickled 30s of 75%, blots the alcohol on bletilla capsule surface with sterilizing filter paper by the bletilla capsule collected on superclean bench by mass percent; With sterile water, bletilla capsule is washed 3 ~ 5 times again, all blot with the liquid of aseptic filter paper by bletilla capsule surface after each washing; Be placed on superclean bench by the bletilla capsule through washing, toast with alcolhol burner naked light flame envelope, the temperature of baking is 400 ~ 500 DEG C, and baking time is 3 ~ 6 seconds; Bletilla capsule through overbaking is positioned in the refrigerator of 4 DEG C and preserves.
2. the store method of bletilla seed according to claim 1, is characterized in that: by the bletilla capsule through overbaking first with on the bale of cotton, refill tying in M.G. pure sulphite paper, be positioned in the refrigerator of 4 DEG C and preserve.
3. the store method of bletilla seed according to claim 1, is characterized in that: during 8 ~ October, gather uncracked bletilla capsule.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210296768.9A CN102792825B (en) | 2012-08-21 | 2012-08-21 | Preservation method for rhizoma bletillae seeds |
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| CN201210296768.9A CN102792825B (en) | 2012-08-21 | 2012-08-21 | Preservation method for rhizoma bletillae seeds |
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| CN102792825A CN102792825A (en) | 2012-11-28 |
| CN102792825B true CN102792825B (en) | 2015-04-01 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012523B (en) * | 2014-05-28 | 2016-03-09 | 中国林业科学研究院亚热带林业研究所 | A kind of encapsulation-vitrification cryopreservation method of bletilla seed |
| CN103999643B (en) * | 2014-06-04 | 2016-06-08 | 湄潭县高岩玄参中药材专业合作社 | The store method of Rheum officinale seed |
| CN104718841A (en) * | 2015-02-26 | 2015-06-24 | 李泽诚 | Treatment method of rhizoma bletillae seeds |
| CN105027886B (en) * | 2015-06-25 | 2017-08-08 | 洪江市金土地生态农业有限责任公司 | A kind of bletilla seed sowing breeding method |
| CN105941392B (en) * | 2016-05-03 | 2018-10-09 | 湖南农业大学 | A kind of bletilla striata capsule store method suitable for tissue-culturing rapid propagation |
| CN106941890A (en) * | 2017-03-23 | 2017-07-14 | 杨军 | A kind of storage method of willow seed |
| CN107549823B (en) * | 2017-09-08 | 2019-12-24 | 四川桑果白兰地农业开发有限公司 | Method for processing mulberry |
| CN107926936A (en) * | 2017-11-30 | 2018-04-20 | 张家界本草科技有限公司 | A kind of bletilla seed cryopreservation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940158A (en) * | 2009-07-09 | 2011-01-12 | 正安县绿野科技产业有限责任公司 | Method for rapidly propagating plantlets by utilizing bletilla striata seeds |
| CN102047783A (en) * | 2009-11-11 | 2011-05-11 | 正安县绿野科技产业有限责任公司 | Storage technique for bletilla seed tubers |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102144541B (en) * | 2010-12-31 | 2012-07-18 | 东莞市睿绅生物技术有限公司 | Three-step method for producing ''dragon head phoenix tail'' high-quality officinal dendrobium stem material |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940158A (en) * | 2009-07-09 | 2011-01-12 | 正安县绿野科技产业有限责任公司 | Method for rapidly propagating plantlets by utilizing bletilla striata seeds |
| CN102047783A (en) * | 2009-11-11 | 2011-05-11 | 正安县绿野科技产业有限责任公司 | Storage technique for bletilla seed tubers |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 贮藏温度对白芨种子寿命的影响;赵仁全等;《贵州农业科学》;20070831;第35卷(第04期);第104-105页 * |
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