CN102786577A - Simple device for preparing plasma protein products through batch adsorption, and method thereof - Google Patents
Simple device for preparing plasma protein products through batch adsorption, and method thereof Download PDFInfo
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Abstract
The invention relates to the fields of the medical and biological technologies, and discloses a simple device for preparing plasma protein products through batch adsorption, and a method thereof. The device comprises an upper end sealing cover (1), a reaction bottle (2), a lower end sealing cover (3), a filter cloth sheet (4) and an openmouthed cover (5). The method is characterized in that two ends of the reaction bottle (2) are sealed through the upper end sealing cover (1), the filter cloth sheet (4) and the lower end sealing cover (3); the addition of a material in the reaction bottle (2) is realized through opening the lower end sealing cover (3); a liquid component in the reaction bottle (2) is filtered by the filter cloth sheet (4) through replacing the upper end sealing cover (5) by the openmouthed cover (5) and through pressurizing the middle part of the reaction bottle (2); and the whole batch adsorption preparation process comprising medium balancing, plasma protein adsorption, other protein washing and target plasma protein eluting is carried out in the reaction bottle (2). The device and the method simplify equipment and operations required by the preparation of the plasma proteins through the batch adsorption, and can simply realize the efficient separation of various plasma proteins. Same-batch experiments enable the screening of various conditions of the batch-adsorption preparation technology to be completed by utilizing a plurality of the devices.
Description
Technical field
The present invention relates to the medical biotechnology field, is the easy device and the method for a kind of batch of formula absorption preparation plasma protein products.
Background technology
The formula of criticizing absorption is plasma proteins industry appellation sanctified by usage; Be one of method for preparing plasma protein products, detailed process is meant: blood plasma or its compositions derived therefrom are rich in multiple plasma proteins, and blood plasma or its compositions derived therefrom are with the medium thorough mixing that has special groups under certain condition; And the purpose plasma proteins is combined with medium; Filter out medium afterwards, carry out again, thereby isolate the process of purpose plasma proteins like washing, wash-out subsequent disposal.This preparation method typically used both domestic and external is exactly the preparation that is used for Prothrombin Complex Concent-.
Along with existing dielectric behavior understand in depth and specificity combines the exploitation of novel medium; The formula absorption of criticizing is applied in the preparation of multiple plasma proteins with the comparatively simple and direct advantage of its treating processes; But with regard to laboratory study, batch formula absorption preparation plasma proteins does not have supporting device and corresponding method thereof so far; This situation has limited batch application of formula absorption method in the plasma protein products separation and Extraction, has also hindered the research that relevant plasma protein products is criticized formula absorption method preparation technology in addition.
Summary of the invention
In view of the foregoing; The object of the present invention is to provide the easy device and the method for a kind of batch of formula absorption preparation plasma protein products; Through these apparatus and method can carry out simply plasma proteinss such as Prothrombin Complex Concent-, PROTEIN C, albumen Z, Protein S, blood coagulation factor VIIIs, effective extraction separation, can accomplish relatively reaching screening simultaneously with batch kinds of processes condition.
Technical scheme:
The present invention is the easy device and the method for a kind of batch of formula absorption preparation plasma protein products.
Said device comprises: upper end closed cover, reaction flask, lower end closed cover, filter cloth sheet and uncovered lid five parts.But said reaction flask is two ends not only can airtight but also opening, the resilient cylindrical plastic of middle portion or rubber material container; Said upper end closed cover and lower end closed cover be with uncovered lid comparatively speaking, be meant that the circular surface of lid is airtight fully; Said uncovered lid is meant that there is circular hole the circular surface centre of lid; Said uncovered cap surface Circularhole diameter is not less than 3/4 of circular lid diameter; Airtight and the opening of said reaction flask through closed cover sealing and unlatching or be replaced by uncovered lid and realize; Said reaction flask open upper end disposes upper end closed cover and uncovered lid; Said reaction flask lower ending opening configuration lower end closed cover; Between said reaction flask open upper end place and upper end closed cover and uncovered lid the filter cloth sheet is arranged; Said filter cloth sheet filter opening is directly looked media particle size and is decided, should be less than 1/3 of media particle size.
Corresponding said apparatus, said method concrete operations are following:
(1) balanced medium
The filter cloth sheet is positioned over the reaction flask open upper end, and upper end closed cover sealed upper end opening is inverted reaction flask.Take by weighing adsorption medium, the amount of taking by weighing is decided according to plasma volume, pours in the reaction flask from lower ending opening, and then adds an amount of balance liquid, carries out balanced medium.Balance finishes, and lower end closed cover sealing lower ending opening is just being put reaction flask, and the upper end closed cover is replaced by uncovered lid, outside filter cloth sheet middle portion is exposed to, is inverted reaction flask again, and to the pressurization of reaction flask middle part, balance liquid leaches through the filter cloth sheet.
(2) plasma proteins absorption
Just putting reaction flask, the uncovered lid in open upper end place is replaced by the upper end sealing cover, is inverted reaction flask, opens the lower end sealing cover, adds blood plasma, the sealing lower ending opening, and this moment, reaction flask was in the two ends air-tight state.The fixation reaction bottle is regulated shaking table temperature and rotating speed in the temperature control shaking table, carries out plasma proteins absorption.Absorption finishes, and is just putting reaction flask, and the upper end closed cover is replaced by uncovered lid, is inverted reaction flask again, to the pressurization of reaction flask middle part, leaches blood plasma.
(3) medium washing
Just putting reaction flask, changing uncovered lid is the upper end closed cover, is inverted reaction flask, opens lower ending opening, adds washings, the closed lower end opening.The fixation reaction bottle is in the temperature control shaking table, and attemperation and rotating speed wash foreign protein.Finish, leach washings by aforesaid operations.
(4) plasma proteins wash-out
Just putting reaction flask, changing uncovered lid is the upper end closed cover, is inverted reaction flask, opens lower ending opening, adds elutriant, the closed lower end opening.The fixation reaction bottle is in the temperature control shaking table, attemperation and rotating speed, wash-out target protein.Finish, leach elutriant, be purpose plasma proteins enriched material by aforesaid operations.
Beneficial effect:
The invention has the beneficial effects as follows; A kind of batch of easy device and method of formula absorption preparation plasma protein products is provided; Through experiment; Can accomplish separation simply and effectively to Prothrombin Complex Concent-, PROTEIN C, albumen Z, Protein S, blood coagulation factor VIII etc.; Wherein prothrombin, VII, IX, X yield can reach respectively in the Prothrombin Complex Concent-: 92.26%, 70.86%, 97.05%, 82.40%, and can reach respectively than living simultaneously: 0.95IU/mg, 0.63IU/mg, 1.05IU/mg, 0.76IU/mg are higher than the " requirement of plasma thromboplastin component ratio work >=0.3IU/mg in Chinese pharmacopoeia (2010 editions) Prothrombin Complex Concent-; The PROTEIN C yield and be respectively 92.20% than living, 1.04IU/mg; The Protein S yield and be respectively 81.00% than living, 1.06IU/mg; Albumen Z yield and ratio are lived and are respectively 88.18%, 4.37 μ g/mg (pressing albumen Z cubage); The blood coagulation factor VIII yield and be respectively 61.30% than living, 1.02IU/mg.In addition, utilize a plurality of these devices, can realize batch same batch of comparison of formula absorption kinds of processes condition, provide powerful support for for batch screening of formula absorption preparation plasma proteins processing condition provides.
Description of drawings:
Fig. 1 is the device appearance synoptic diagram.
Fig. 2 is the device decomposing schematic representation.
Fig. 3 is a filter cloth sheet vertical view.
The uncovered lid vertical view of Fig. 4.
Among the figure: 1. upper end closed cover; 2. reaction flask; 3. lower end closed cover; 4. filter cloth sheet; 5. uncovered lid.
Embodiment
In conjunction with accompanying drawing and embodiment the present invention is elaborated, but embodiment of the present invention is not limited thereto.
Embodiment 1: the Prothrombin Complex Concent-preparation experiment
(1) balanced medium
Place filter cloth sheet 4 in reaction flask 2 open upper end places,, be inverted reaction flask 2 with closed cover 1 sealed upper end opening.Take by weighing 0.15g DEAE-Sephedex A50 medium, measure the 80ml balance liquid, the lower ending opening place is poured in the reaction flask 2 successively, and said balance liquid is the sodium citrate soln of pH6.8, contains the NaCl of 0.05mol/L.After mixing, 4～8 ℃ of conditions are carried out DEAE-Sephedex A50 balanced medium.Behind the 24h, closed cover 3 sealing lower ending openings are just being put reaction flask 2, and upper end closed cover 1 is replaced by uncovered lid 5, is inverted reaction flask 2, the pressurization of reaction flask 2 middle parts, and balance liquid leaches through filter cloth sheet 4; Just putting reaction flask 2, uncovered lid 5 is replaced by upper end closed cover 1, is inverted reaction flask 2, opens lower ending opening, adds the 80ml balance liquid again to DEAE-Sephedex A50 balanced medium, leaches by aforesaid operations behind the 24h.
(2) absorption of prothrombin, VII, IX, X
Get 100ml healthy subjects pooled plasma, lower ending opening is poured reaction flask 2 into, closed cover 3 sealing lower ending openings, and fixation reaction bottle 2 is in the temperature control shaking table, and attemperation is 4 ℃, and rotating speed is 200rpm, absorption 40min.Absorption finishes, and changing upper end closed cover 1 is uncovered lid 5, leaches and mixes slurry.
(3) foreign protein washing
Get the 50ml washings respectively, said washings is the sodium citrate soln of pH7.0, contains the NaCl of 0.1mol/L, by the adsorption process operation, and washing DEAE-Sephedex A50 medium 2 times, each 20min leaches washings.
(4) wash-out of prothrombin, VII, IX, X
Get the 25ml elutriant respectively, said elutriant is the sodium citrate soln of pH7.2, contains the NaCl of 2.4mol/L, by the adsorption process operation, and wash-out 2 times, each 15min.
Collect elutriant, obtain the Prothrombin Complex Concent-enriched material, the yield of its prothrombin, VII, IX, X with see table 1 than living.
The yield of four kinds of main thrombin of table 1 Prothrombin Complex Concent-enriched material and ratio are lived
| Type | Yield (%) | Than live (IU/mg) |
| Prothrombin | 92.26 | 0.95 |
| Proconvertin | 70.86 | 0.63 |
| Plasma thromboplastin component | 97.05 | 1.05 |
| Factor X | 82.40 | 0.76 |
Embodiment 2: PROTEIN C separation and purification experiment
(1) balanced medium
Take by weighing 0.3g DEAE-Sephedex A50 medium, get the 300ml balance liquid respectively, said balance liquid is the pH7.0 sodium citrate soln, contains the NaCl of 0.05mol/L.Press 2 balances of (1) operation completion DEAE-Sephedex A50 medium among the embodiment 1.
(2) absorption of PROTEIN C
Get 100ml healthy subjects pooled plasma; The shaking table temperature is 4 ℃, and rotating speed is 250rpm, 50min.Press the absorption of (2) operation completion PROTEIN C among the embodiment 1.
(3) foreign protein washing
Get the 150ml washings respectively, said washings is the pH7.4 sodium citrate soln, contains the NaCl of 0.1mol/L.Press 2 washings of (3) operation completion foreign protein among the embodiment 1, each 30min.
(4) wash-out of PROTEIN C
Get the 50ml elutriant respectively, said elutriant is the sodium citrate soln of pH7.4, contains 1.5mol/L NaCl.Press 2 wash-outs of (4) operation completion PROTEIN C among the embodiment 1, each 20min.
Collect elutriant, obtain the PROTEIN C enriched material, its PROTEIN C yield and ratio are lived and are seen table 2.
The yield of PROTEIN C and ratio are lived in the table 2 PROTEIN C enriched material
| Type | Yield (%) | Than live (IU/mg) |
| PROTEIN C | 92.20 | 1.04 |
Embodiment 3: Protein S separation and purification experiment
(1) balanced medium
Take by weighing 0.2g DEAE-Sephedex A50 medium, get the 120ml balance liquid respectively, said balance liquid is the pH7.0 sodium citrate soln, contains the NaCl of 0.075mol/L.Press 2 balances of (1) operation completion DEAE-Sephedex A50 medium among the embodiment 1.
(2) absorption of Protein S
Get 100ml healthy subjects pooled plasma; The shaking table temperature is 4 ℃, and rotating speed is 230rpm, 40min.Press the absorption of (2) operation completion Protein S among the embodiment 1.
(3) foreign protein washing
Get the 40ml washings respectively, said washings is the sodium citrate soln of pH7.0, contains the NaCl of 0.1mol/L.Press 2 washings of (3) operation completion foreign protein among the embodiment 1, each 30min.
(4) wash-out of Protein S
Get the 25ml elutriant respectively, said elutriant is the sodium citrate soln of pH7.0, contains the NaCl of 1.6mol/L.Press 2 wash-outs of (4) operation completion Protein S among the embodiment 1, each 15min.
Collect elutriant, obtain the Protein S enriched material, its Protein S yield and ratio are lived and are seen table 3.
The yield of Protein S and ratio are lived in the table 3 Protein S enriched material
| Type | Yield (%) | Than live (IU/mg) |
| Protein S | 81.00 | 1.06 |
Embodiment 4: albumen Z separation and purification experiment
(1) balanced medium
Take by weighing 0.25g DEAE-Sephedex A50 medium, get the 150ml balance liquid respectively, said balance liquid is the pH7.0 sodium citrate soln, contains the NaCl of 0.075mol/L.Press 2 balances of (1) operation completion DEAE-Sephedex A50 medium among the embodiment 1.
(2) absorption of albumen Z
Get 100ml healthy subjects pooled plasma; The shaking table temperature is 4 ℃, and rotating speed is 220rpm, 40min.Press the absorption of (2) operation completion albumen Z among the embodiment 1.
(3) foreign protein washing
Get the 100ml washings respectively, said washings is the sodium citrate soln of pH7.0, contains the NaCl of 0.1mol/L.Press 2 washings of (3) operation completion foreign protein among the embodiment 1, each 25min.
(4) wash-out of albumen Z
Get the 30ml elutriant respectively, said elutriant is the sodium citrate soln of pH7.0, contains the NaCl of 1.6mol/L.Press 2 wash-outs of (4) operation completion albumen Z among the embodiment 1, each 15min.
Collect elutriant, obtain albumen Z enriched material, its albumen Z yield and ratio are lived and are seen table 4.
The yield of albumen Z and ratio are lived in the table 4 albumen Z enriched material
| Type | Yield (%) | Than live (μ g/mg) |
| Albumen Z | 88.18 | 4.37 |
(1) the synthetic CGA-15 balanced medium of self-control
Take by weighing the synthetic CGA-15 medium of 10g self-control, get the 200ml balance liquid respectively, said balance liquid is the sodium citrate soln of pH6.5.Room temperature condition is pressed 3 balances of (1) operation completion CGA-15 medium among the embodiment 1.
(2) absorption of blood coagulation factor VIII
Get 80ml healthy subjects pooled plasma; The shaking table temperature is 20 ℃, and rotating speed is 220rpm, 30min.Press the absorption of (2) operation completion blood coagulation factor VIII among the embodiment 1.
(3) foreign protein washing
Get the 100ml balance liquid respectively, press 2 washings of (3) operation completion foreign protein among the embodiment 1, each 15min.
(4) wash-out of blood coagulation factor VIII
Get the 40ml elutriant respectively, said elutriant is pH7.0, the solution of 0.6mol/L concentration NaCl.Press 2 wash-outs of (4) operation completion blood coagulation factor VIII among the embodiment 1, each 20min.
Collect elutriant, obtain the blood coagulation factor VIII enriched material, its blood coagulation factor VIII yield and ratio are lived and are seen table 5.
The yield of table 5 blood coagulation factor VIII enriched material blood coagulation factor VIII and ratio are lived
| Type | Yield (%) | Than live (IU/mg) |
| Blood coagulation factor VIII | 61.30 | 1.02 |
The different elution requirement screening experiments of embodiment 6 Protein S
(1) balanced medium
Get 9 covering devices, take by weighing 9 parts of 0.2g DEAE-Sephedex A50 media respectively, get 9 portions of 120ml balance liquids respectively, said balance liquid is the pH6.8 sodium citrate soln, contains the NaCl of 0.05mol/L.Press among the embodiment 1 (1) operation and accomplish 2 balances of DEAE-Sephedex A50 medium in 9 covering devices.
(2) absorption of Protein S
Get 9 parts of 120ml healthy subjects pooled plasmas; The shaking table temperature is 4 ℃, and rotating speed is 220rpm, 50min.9 covering devices all are fixed in same shaking table, press the absorption of (2) operation completion Protein S among the embodiment 1.
(3) foreign protein washing
Get 9 parts of 40ml washingss respectively, said washings is the sodium citrate soln of pH7.2, contains the NaCl of 0.1mol/L.Accomplish 2 washings of foreign protein, each 20min respectively by (3) operation among the embodiment 1.
(4) wash-out of Protein S
Get 9 parts of 30ml elutriants respectively, said elutriant composition is seen table 6.Operate 2 wash-outs accomplishing Protein S respectively by (4) among the embodiment 1, each 15min.
Collect elutriant respectively, obtain the Protein S enriched material, its Protein S yield and ratio are lived and are seen table 7.
Table 69 kind of Protein S elutriant
The yield of Protein S and ratio are lived in the table 7 Protein S enriched material
| The elution requirement type | Yield (%) | Than live (IU/mg) |
| One | 80.55 | 0.63 |
| Two | 63.01 | 0.57 |
| Three | 64.33 | 0.63 |
| Four | 58.61 | 0.45 |
| Five | 44.53 | 0.43 |
| Six | 42.16 | 0.41 |
| Seven | 87.26 | 0.84 |
| Eight | 67.21 | 0.59 |
| Nine | 53.58 | 0.61 |
Claims (4)
1. the easy device of one kind batch of formula absorption preparation plasma protein products is characterized in that: comprise upper end closed cover, reaction flask, lower end closed cover, filter cloth sheet and uncovered lid five parts.
2. according to the easy device of the said a kind of easy batch of formula absorption preparation plasma protein products of claim 1, it is characterized in that: but reaction flask be two ends not only can airtight but also opening, the resilient cylindrical plastic of middle portion or rubber material container; The airtight sealing through closed cover of reaction flask realizes; The opening of reaction flask is opened through closed cover or closed cover is replaced by uncovered lid realization; Uncovered lid is the middle round-meshed bottle cap of the circular surface of lid; The filter cloth sheet is middle 1～3 metafiltration cloth, and the edge is with rigid plastics ring or rubber ring fixed disk shape structure; The filter cloth sheet is embedded in the middle of reaction flask open upper end and upper end closed cover or the uncovered lid.
3. a batch formula that designs according to claim 1 is adsorbed the simple and easy method for preparing plasma protein products, is primarily characterized in that: through the sealed at both ends of lower end closed cover, upper end closed cover and filter cloth sheet realization response bottle; Be inverted reaction flask and open the lower end closed cover, the interpolation of realization response vial material; The upper end closed cover is replaced by uncovered lid and to the pressurization of reaction flask middle part, liquid component leaches through the filter cloth sheet in the reaction flask; Balanced medium, plasma proteins absorption, foreign protein washing, whole batch of formula absorption of purpose plasma proteins wash-out preparation process are all carried out in reaction flask; Reaction flask can be accomplished plasma proteins absorption, medium washing and plasma proteins elution process in shaking table.
4. according to easy device and the method said plasma proteins of claim 1, it is characterized in that: comprise people and other animals with 3 said a kind of batch of formula absorption preparation plasma protein products.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965303A (en) * | 2020-07-25 | 2020-11-20 | 山东泰邦生物制品有限公司 | Device for batch type adsorption separation test and using method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3560066B2 (en) * | 1993-12-17 | 2004-09-02 | 三菱ウェルファーマ株式会社 | Method for fractionating plasma protein |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN101444757A (en) * | 2007-08-14 | 2009-06-03 | 米利波尔有限公司 | Porous sorptive medium, sorption device containing the medium and chromatography system |
-
2012
- 2012-07-20 CN CN201210252349.5A patent/CN102786577B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3560066B2 (en) * | 1993-12-17 | 2004-09-02 | 三菱ウェルファーマ株式会社 | Method for fractionating plasma protein |
| CN2911683Y (en) * | 2006-07-11 | 2007-06-13 | 北京丰杰华信科学仪器有限公司 | Gel chromatographic column |
| CN101444757A (en) * | 2007-08-14 | 2009-06-03 | 米利波尔有限公司 | Porous sorptive medium, sorption device containing the medium and chromatography system |
Non-Patent Citations (6)
| Title |
|---|
| 《血浆蛋白分离纯化的研究进展》 20040731 金晓霞等 血浆蛋白分离纯化的进展-- 亲和技术的重要作用 第74-79页 1-2 第24卷, 第7期 * |
| BOSCHETTI E: "液相层析法在人血浆蛋白纯化中的应用", 《国外医学输血及血液学分册》, vol. 12, no. 2, 30 April 1989 (1989-04-30), pages 93 * |
| BURNOUF T: "用层析法分离人血浆蛋白", 《用层析法分离人血浆蛋白》, vol. 12, no. 2, 30 April 1989 (1989-04-30), pages 94 * |
| J. HEYST等: "Contributions to the Optimal Use of Human Blood.11. The Large-Scale Preparation of Prothrombin Complex.A Comparison between Two Methods Using the Anion Exchangers DEAE-Cellulose DE 52 and DEAE-Sephadex A-50", 《VOX SANG》, 31 August 1973 (1973-08-31) * |
| 邱家山等: "人凝血酶原复合物分离纯化工艺的研究进展", 《生物技术通报》, no. 2, 30 April 2006 (2006-04-30), pages 18 - 1 * |
| 金晓霞等: "血浆蛋白分离纯化的进展—— 亲和技术的重要作用", 《血浆蛋白分离纯化的研究进展》, vol. 24, no. 7, 31 July 2004 (2004-07-31), pages 74 - 79 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965303A (en) * | 2020-07-25 | 2020-11-20 | 山东泰邦生物制品有限公司 | Device for batch type adsorption separation test and using method |
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