CN208959375U - A kind of chromatography column apparatus for plasma protein separation - Google Patents
A kind of chromatography column apparatus for plasma protein separation Download PDFInfo
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- CN208959375U CN208959375U CN201821372892.8U CN201821372892U CN208959375U CN 208959375 U CN208959375 U CN 208959375U CN 201821372892 U CN201821372892 U CN 201821372892U CN 208959375 U CN208959375 U CN 208959375U
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- plasma protein
- screen assemblies
- protein separation
- chromatography column
- column apparatus
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Abstract
The utility model discloses a kind of chromatography column apparatus for plasma protein separation, including chromatographing cylinder, wherein, screen assemblies are provided in the chromatographic column body, the screen assemblies are annular in shape, and the centre of the screen assemblies is provided with sealing bolt, and stainless steel pallet is provided below in the screen assemblies, the screen assemblies include upper side frame, non-woven fabrics filter mesh layer and deflector, and the excircle of the upper side frame turns down surround non-woven fabrics filter mesh layer and deflector outward.Chromatography column apparatus provided by the utility model for plasma protein separation, is not only able to greatly improve the utilization rate of expensive raw materials, avoids gel particle expansion blockage mesh, and be easily installed replacement screen assemblies, substantially reduce use cost.
Description
Technical field
The utility model relates to a kind of mixture gel decker more particularly to a kind of layers for plasma protein separation
Analyse column device.
Background technique
Chromatographic column is the main body in gel chromatography technology, generally uses glass tube or lucite tube.According to sample mixture
Middle each component is different with distribution coefficient in mobile phase in stationary phase, thus a kind of chromatographic column for plasma protein separation of separation
Device.The diameter of chromatographic column does not influence separating degree, and amount of samples is big, can increase the diameter of column, is typically prepared with gel column,
Diameter is greater than 20 centimetres, but sample should be uniformly distributed on gel column bed surface in sample-adding.
With sieve plate isolation gel particle, (gel is for adsorbing spy to the existing chromatography column apparatus for plasma protein separation
Determine plasma protein, then elution is collected) in pillar, in order to avoid leak down.Being fixedly mounted on chromatographic column originally, by more mesh
Partition made of stainless steel mesh multiple-layer stacked, being used multiple times (can be used many times, until gap is plugged and leads to flow velocity
It is abandoned after slow enough).Because gel particle acid-base balance and washing elution plasma protein during, leakage between stainless steel sieve or
Gap is blocked after gel particle expansion between gap, final plasma flow rate slows down, and influences efficiency.
Dead volume under chromatographic column filter plate should be small as far as possible, if the dead volume under support filter plate is big, by separation component
Between a possibility that re-mixing with regard to big, as a result, influencing elution peak shape, there is trailing phenomenon, reduce separating effect.In essence
Really when separation, 1/1000 of dead volume no more than total bed volume.Therefore, always for the chromatography column apparatus of plasma protein separation
It is the primary study object of this field, and is persistently improving.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of chromatography column apparatus for plasma protein separation, no
But the utilization rate that can greatly improve expensive raw materials avoids gel particle expansion blockage mesh, and is easily installed replacement sieve
Screen component substantially reduces use cost.
The utility model is to solve above-mentioned technical problem and the technical solution adopted is that provide a kind of for plasma protein point
From chromatography column apparatus, including chromatography cylinder, wherein screen assemblies are provided in the chromatographic column body, the screen assemblies are in
Annular shape, the centre of the screen assemblies are provided with sealing bolt, and stainless steel pallet, the sieve is provided below in the screen assemblies
Screen component includes upper side frame, non-woven fabrics filter mesh layer and deflector, and the excircle of the upper side frame turns down surround non-woven fabrics outward
Filter mesh layer and deflector.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein the non-woven fabrics filter mesh layer is terylene silk, institute
The aperture for stating terylene silk is 200 mesh.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein pass through card between the upper side frame and deflector
Mouth fastening terylene silk；It is equipped with sealing ring item between the upper side frame and terylene silk, leads between the upper side frame and deflector
Screw locking is crossed to fix and be sealed at contact gap using plastics welding.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein multiple through-holes are distributed on the deflector, and
Via densities at deflector center and excircle are greater than the via densities positioned at deflector intermediate region.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein the chromatography cylinder is lucite cylinder, described
Lucite cylinder is set on carrier base, and agitating shaft is additionally provided in the lucite cylinder, and one end of the agitating shaft is equipped with stirring
Impeller, the other end are connected with the motor on carrier base, between the agitator arm is located at the top of screen assemblies and keeps certain
Gap.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein the carrier base includes top cover and pedestal, described
Top cover and pedestal be it is discoid, pass through several column branch around lucite cylinder circle distribution between the top cover and pedestal
Bar is connected.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein the material of the sealing bolt is polyether-ether-ketone tree
Rouge.
The above-mentioned chromatography column apparatus for plasma protein separation, wherein the screen assemblies are plastic mesh component.
The utility model comparison prior art has following the utility model has the advantages that provided by the utility model for plasma protein point
From chromatography column apparatus, gel particle and blood plasma are sufficiently mixed by agitating device, to improve gel particle adsorbed target blood plasma
Albumen greatly improves the utilization rate of expensive raw materials；Stainless steel mesh is replaced using non-woven fabrics filter mesh layer, utilizes nonwoven after tensioning
The characteristic of cloth effectively avoids gel particle expansion from blocking blocking mesh.In addition, non-woven fabrics filter mesh layer cooperation upper side frame and deflector
Flexible sieve component is formed, installation replacement is not only easy to, substantially reduces and cost is replaced, and product can be mitigated significantly
Overall weight, and integral strength with higher and leakproofness.
Detailed description of the invention
Fig. 1 is the chromatography column apparatus structural schematic diagram that the utility model is used for plasma protein separation；
Fig. 2 is the utility model for the non-woven fabrics filter mesh layer structural representation in the chromatography column apparatus of plasma protein separation
Figure.
In figure:
1 chromatography 2 screen assemblies of cylinder, 3 sealing bolt
4 carrier base, 5 agitating shaft, 6 agitator arm
7 motor, 8 column strut, 21 non-woven fabrics filter mesh layer
22 upper side frame, 23 deflector, 24 through-hole
Specific embodiment
The utility model will be further described with reference to the accompanying drawings and examples.
Fig. 1 is the chromatography column apparatus structural schematic diagram that the utility model is used for plasma protein separation；Fig. 2 is the utility model
For the non-woven fabrics strainer schematic diagram of a layer structure in the chromatography column apparatus of plasma protein separation.
Referring to Figure 1 and Fig. 2, the chromatography column apparatus provided by the utility model for plasma protein separation, including chromatography
Cylinder 1 is provided with screen assemblies 2 in the chromatography cylinder 1, and the screen assemblies 2 are annular in shape, in the screen assemblies 2
Between be provided with sealing bolt 3, the screen assemblies 2 are provided below stainless steel pallet, the screen assemblies 2 include upper side frame 22,
Non-woven fabrics filter mesh layer 21 and deflector 23, the excircle of the upper side frame 22 turns down outward to be surrounded non-woven fabrics filter mesh layer 21 and leads
Flowing plate 23.
Chromatography column apparatus provided by the utility model for plasma protein separation, the non-woven fabrics filter mesh layer 21 are terylene
Silk, the aperture of the terylene silk are 200 mesh.Terylene thin,tough silk is fastened by bayonet between the upper side frame 22 and deflector 23
Cloth, so that terylene silk is in tensioning state；To effectively avoid gel particle swollen using the elastic deformation of non-woven fabrics after tensioning
Swollen blocking mesh.Since elasticity of plastics is preferable, it is convenient for so that non-woven fabrics filter mesh layer 21 is in tensioning state, therefore preferably plastics material
The upper side frame 22 and deflector 23 of matter.Certainly, wood materials or metal also can be selected in upper side frame 22 and deflector 23, as long as cooperation
21 use of non-woven fabrics filter mesh layer.Sealing ring item, 22 He of upper side frame are equipped between the upper side frame 22 and terylene silk
It is fixed between deflector 23 by screw locking and is sealed at contact gap using plastics welding；So as to subtract significantly
The overall weight of light product, and integral strength with higher and leakproofness.Multiple through-holes 24 are distributed on the deflector 23,
And it is located at the via densities at 23 center of deflector and excircle and is greater than the via densities for being located at 23 intermediate region of deflector；It is described
The material of sealing bolt 3 is polyether-ether-ketone resin (PEEK).
Chromatography column apparatus provided by the utility model for plasma protein separation, wherein the chromatography cylinder 1 is organic
Glass infuser, the lucite cylinder are set on carrier base 4, and agitating shaft 5, the agitating shaft 5 are additionally provided in the lucite cylinder
One end be equipped with agitator arm 6, the other end is connected with the motor 7 on carrier base 4, smashes gel by stirring, greatly improves high
The utilization rate of your raw material.The agitator arm 6 is located at the top of screen assemblies 2 and keeps certain gap, avoids encountering sieve
Screen component.The carrier base 4 includes top cover and pedestal, the top cover and pedestal be it is discoid, motor 7 is set on top cover；Institute
It states and is connected between top cover and pedestal by several column struts 8 around lucite cylinder circle distribution, thus can be well
It plays a supporting role, and will not influence the separation situation of observation lucite cylinder plasma protein.
Chromatography column apparatus provided by the utility model for plasma protein separation, is replaced not using non-woven fabrics filter mesh layer 21
Become rusty steel sieve, is not only able to avoid gel particle expansion blockage mesh, and be easily installed replacement screen assemblies, substantially reducing makes
Use cost.When non-woven fabrics filter mesh layer is when soda acid situation lower pulling force and toughness decline, does not then use, replace nonwoven cloth material.
Stainless steel processing cost is necessarily higher than non-woven fabrics.Under square one, production cost is reduced.
Chromatography column apparatus provided by the utility model for plasma protein separation, is tested using spun silk, by repeatedly sour
Alkali process and loading test observe spun silk profile variation shape after load-carrying.After repetitive process operating operation test, for the first time to
Three times, shape is unchanged after observation spun silk is pressurized, and shape has shrinkage variation after the 4th spun silk compression, after the 5th spun silk compression
Shape has obvious shrinkage to change.It can be seen that the 4th spun silk, after counterweight carries pressure, spun silk has been not suitable for using, and therefore, sieve plate
Selection is feasible program using abandoning after three batch processes.
Although the utility model is disclosed as above with preferred embodiment, so it is not intended to limit the utility model, any
Those skilled in the art, without departing from the spirit and scope of the utility model, when can make a little modification and it is perfect, therefore this
The protection scope of utility model, which is worked as, to be subjected to the definition of the claims.
Claims (8)
1. a kind of chromatography column apparatus for plasma protein separation, including chromatography cylinder, which is characterized in that in the chromatographic column body
Screen assemblies are provided with, the screen assemblies are annular in shape, and the centre of the screen assemblies is provided with sealing bolt, the screening group
Stainless steel pallet is provided below in part, and the screen assemblies include upper side frame, non-woven fabrics filter mesh layer and deflector, the upper side frame
Excircle turn down surround non-woven fabrics filter mesh layer and deflector outward.
2. the chromatography column apparatus for plasma protein separation as described in claim 1, which is characterized in that the non-woven fabrics strainer
Layer is terylene silk, and the aperture of the terylene silk is 200 mesh.
3. the chromatography column apparatus for plasma protein separation as claimed in claim 2, which is characterized in that the upper side frame and lead
Terylene silk is fastened by bayonet between flowing plate；Sealing ring item, the upper side frame are equipped between the upper side frame and terylene silk
It is fixed between deflector by screw locking and is sealed at contact gap using plastics welding.
4. the chromatography column apparatus for plasma protein separation as described in claim 1, which is characterized in that divide on the deflector
Multiple through-holes are furnished with, and is located at the via densities at deflector center and excircle and is greater than the through-hole for being located at deflector intermediate region
Density.
5. the chromatography column apparatus for plasma protein separation as described in claim 1, which is characterized in that the chromatography cylinder is
Lucite cylinder, the lucite cylinder are set on carrier base, and agitating shaft, the agitating shaft are additionally provided in the lucite cylinder
One end be equipped with agitator arm, the other end is connected with the motor on carrier base, and the agitator arm is located at the top of screen assemblies
And keep certain gap.
6. the chromatography column apparatus for plasma protein separation as claimed in claim 5, which is characterized in that the carrier base includes
Top cover and pedestal, the top cover and pedestal be it is discoid, between the top cover and pedestal by it is several surround lucite cylinder
The column strut of circle distribution is connected.
7. the chromatography column apparatus for plasma protein separation as described in claim 1, which is characterized in that the material of the sealing bolt
Matter is polyether-ether-ketone resin.
8. the chromatography column apparatus for plasma protein separation as described in claim 1, which is characterized in that the screen assemblies are
Plastic mesh component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821372892.8U CN208959375U (en) | 2018-08-24 | 2018-08-24 | A kind of chromatography column apparatus for plasma protein separation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821372892.8U CN208959375U (en) | 2018-08-24 | 2018-08-24 | A kind of chromatography column apparatus for plasma protein separation |
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| Publication Number | Publication Date |
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| CN208959375U true CN208959375U (en) | 2019-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201821372892.8U Active CN208959375U (en) | 2018-08-24 | 2018-08-24 | A kind of chromatography column apparatus for plasma protein separation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112574979A (en) * | 2020-12-14 | 2021-03-30 | 北京华创精科生物技术有限公司 | Gel treatment equipment and method for plasma |
-
2018
- 2018-08-24 CN CN201821372892.8U patent/CN208959375U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112574979A (en) * | 2020-12-14 | 2021-03-30 | 北京华创精科生物技术有限公司 | Gel treatment equipment and method for plasma |
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