CN102782153B - Aad-12大豆事件416的检测 - Google Patents

Aad-12大豆事件416的检测 Download PDF

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Publication number
CN102782153B
CN102782153B CN201080062204.8A CN201080062204A CN102782153B CN 102782153 B CN102782153 B CN 102782153B CN 201080062204 A CN201080062204 A CN 201080062204A CN 102782153 B CN102782153 B CN 102782153B
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China
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event
probe
primer
soybean
dna
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Expired - Fee Related
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CN201080062204.8A
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English (en)
Chinese (zh)
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CN102782153A (zh
Inventor
S·诺瓦克
Y·C·崔
T·W·格里尼
N·周
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Corteva Agriscience LLC
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Dow AgroSciences LLC
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Priority claimed from PCT/US2010/057967 external-priority patent/WO2011066360A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/114—Reactions characterised by their quantitative nature the purpose being quantitative analysis involving a quantitation step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201080062204.8A 2009-11-24 2010-11-24 Aad-12大豆事件416的检测 Expired - Fee Related CN102782153B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US26395009P 2009-11-24 2009-11-24
US61/263,950 2009-11-24
US32736910P 2010-04-23 2010-04-23
US61/327,369 2010-04-23
PCT/US2010/057967 WO2011066360A1 (en) 2009-11-24 2010-11-24 Detection of aad-12 soybean event 416

Publications (2)

Publication Number Publication Date
CN102782153A CN102782153A (zh) 2012-11-14
CN102782153B true CN102782153B (zh) 2015-08-12

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CN201080062204.8A Expired - Fee Related CN102782153B (zh) 2009-11-24 2010-11-24 Aad-12大豆事件416的检测

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US (1) US8685677B2 (enExample)
EP (1) EP2504456B1 (enExample)
JP (1) JP5907885B2 (enExample)
KR (1) KR20120107974A (enExample)
CN (1) CN102782153B (enExample)
AR (1) AR079150A1 (enExample)
AU (1) AU2010324818B2 (enExample)
CA (1) CA2781622A1 (enExample)
CL (1) CL2012001353A1 (enExample)
CO (1) CO6561770A2 (enExample)
IL (1) IL219932A0 (enExample)
IN (1) IN2012DN04901A (enExample)
MX (1) MX2012006074A (enExample)
RU (1) RU2573898C2 (enExample)
UY (1) UY33058A (enExample)
ZA (1) ZA201203713B (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104928256A (zh) 2009-11-24 2015-09-23 陶氏益农公司 Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定
CA2962242A1 (en) 2014-09-29 2016-04-07 Agrigenetics, Inc. Low lignin non-transgenic alfalfa varieties and methods for producing the same
CA3045784A1 (en) 2016-10-11 2018-05-17 Dow Agrosciences Llc Modulation of transgene expression in plants
CN116144671B (zh) * 2022-09-23 2025-07-11 隆平生物技术(海南)有限公司 转基因玉米事件lp026-3及其检测方法
CN116676417B (zh) * 2023-07-24 2023-09-29 捷康生物科技(海南)有限公司 转基因大豆事件jk1001-3及其检测方法
CN116694813B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-1及其检测方法
CN116694812B (zh) * 2023-07-25 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp086-2及其检测方法
CN116694815B (zh) * 2023-08-01 2023-10-03 隆平生物技术(海南)有限公司 转基因大豆事件lp012-2及其检测方法
CN116694814B (zh) * 2023-08-01 2023-10-10 隆平生物技术(海南)有限公司 转基因大豆事件lp012-1及其检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1370841A (zh) * 2001-02-26 2002-09-25 安利忻 基因改造农作物及其加工产品的定量检测方法和试剂盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060127889A1 (en) * 2000-07-25 2006-06-15 Dotson Stanton B Method for assessing transgene expression and copy number
US7955800B2 (en) * 2002-06-25 2011-06-07 Advpharma Inc. Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients
RU2270254C2 (ru) * 2004-04-30 2006-02-20 Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
EP2484202B1 (en) * 2005-10-28 2017-06-07 Dow AgroSciences LLC Novel herbicide resistance genes
TWI412591B (zh) * 2007-04-20 2013-10-21 Sigma Tau Rare Diseases S A 穩定的重組腺苷脫胺酶
CN104928256A (zh) * 2009-11-24 2015-09-23 陶氏益农公司 Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1370841A (zh) * 2001-02-26 2002-09-25 安利忻 基因改造农作物及其加工产品的定量检测方法和试剂盒

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Publication number Publication date
AU2010324818B2 (en) 2015-09-17
MX2012006074A (es) 2012-06-19
KR20120107974A (ko) 2012-10-04
AU2010324818A1 (en) 2012-06-14
EP2504456B1 (en) 2017-12-20
EP2504456A1 (en) 2012-10-03
CO6561770A2 (es) 2012-11-15
JP5907885B2 (ja) 2016-04-26
IL219932A0 (en) 2012-07-31
AR079150A1 (es) 2011-12-28
CN102782153A (zh) 2012-11-14
RU2573898C2 (ru) 2016-01-27
UY33058A (es) 2011-06-30
ZA201203713B (en) 2013-01-30
IN2012DN04901A (enExample) 2015-09-25
JP2013512661A (ja) 2013-04-18
EP2504456A4 (en) 2014-04-09
CL2012001353A1 (es) 2012-10-05
RU2012126088A (ru) 2013-12-27
US20130029329A1 (en) 2013-01-31
US8685677B2 (en) 2014-04-01
CA2781622A1 (en) 2011-06-03

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