CN102771779A - Ganoderma sinensis health product prepared by submerged fermentation and preparation method thereof - Google Patents
Ganoderma sinensis health product prepared by submerged fermentation and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a Ganoderma sinensis health product prepared by submerged fermentation and a preparation method thereof. The preparation method includes the steps of A, inoculating Ganoderma sinensis to culture solution, and performing shake culture on a shaker to obtain fungus solution; B, inoculating the fungus solution obtained in the step A to liquid medium containing barely malt and soybean; and performing aerobic culture to obtain fermented Ganoderma sinensis mycelia and Ganoderma sinensis fermentation broth; C, subjecting the Ganoderma sinensis mycelia obtained in the step B to cell tissue breakage, and extracting with water to obtain Ganoderma sinensis mycelia extract; and D, mixing the Ganoderma sinensis fermentation broth obtained in the step B and the Ganoderma sinensis mycelia extract obtained in the step C, and concentrating obtained liquor to obtain the Ganoderma sinensis health product. By the industrial submerged fermentation technology, the Ganoderma sinensis culture can be propagated industrially and massively in the liquid medium. Therefore, the process is simple, and the cost is low.
Description
Technical field
The present invention relates to a kind of health products and preparation method thereof, particularly relate to a kind of purple sesame health products that pass through the liquid deep layer fermenting preparation and preparation method thereof.
Background technology
Purple sesame (formal name used at school: Ganoderma lucidum; Japonicum; Lobatum; Sinense; Sp.) also claim " Chinese glossy ganoderma ", it is sweet to distinguish the flavor of, and property is flat.Mainly contain ergosterol, organic acid, Glucosamine, polysaccharide, resin, sweet mellow wine, polysaccharide polyol, aliphatic acid, contain alkaloid, lactone, coumarin, water soluble protein and multiple enzyme again.The range of application of purple sesame is very extensive, and no matter the heart, lung, the dirty weakness of liver,spleen,kidney all can be taken.Purple sesame is cured the disease to plant and relates to each systems such as breathing, circulation, digestion, nerve, endocrine and immunity; Contain inside and outside, woman, youngster, each section's disease of face.Its basic reason just is that purple sesame strengthens the body resistance to consolidate the constitution, raise immunity, the huge effect that improves Abwehrkraft des Koepers.It is different from general medicine and certain disease is played therapeutic action; Also being different from general nutritional health food only replenishes and strengthens the deficiency of nutrient in a certain respect; But bidirectional modulation function of human body balance is on the whole transferred the internal body vigor, regulates human body metabolism function; Improve the autoimmunity ability, impel whole internal organ or organ function normalization.
At present, correlative study both domestic and external mainly concentrates on extraction and pharmacology, the drug effect of purple sesame function factor.But the artificial cultivation field in purple sesame fructification but receives a lot of technical limitations, carries out suitability for industrialized production and civil nature use widely seldom on a large scale, and simultaneously, this technical field also is difficult to commercialization and uses.Before 10 years, the year recoverable amount of the purple sesame fructification of occurring in nature has 10,000 tons approximately, 5 kilotons but present recoverable amount is but only had an appointment; And; As stated, purple sesame fructification is difficult to a large amount of manual work production again owing to technical reason; Therefore, people have produced significant contradiction to the shortage of craving for purple sesame fructification recoverable amount of purple sesame health-care efficacy and therapeutic efficiency.
Summary of the invention
The objective of the invention is to; Overcome the defective of above-mentioned prior art and a kind of suitability for industrialized production of can carrying out on a large scale is provided, and can produce the method that liquid deep layer fermenting prepares purple sesame health products of passing through of the higher purple sesame fructification of effectiveness with lower cost.
For realizing above-mentioned purpose, the present invention takes following design:
Of the present inventionly a kind ofly prepare the method for purple sesame health products, comprise the steps: through liquid deep layer fermenting
A, purple sesame bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation;
C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains purple camphorata mycelium extract;
D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain purple sesame health products.
Preferably, in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
More preferably, the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
More preferably, in said step D, the mutual mixed pH value of purple sesame fermented liquid and purple camphorata mycelium extract is 6～7, and the refractive power pol is 2～6%.
Another object of the present invention is to provide a kind of purple sesame health products through the liquid deep layer fermenting preparation, said purple sesame health products are to be prepared from following method:
A, purple sesame bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation;
C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains purple camphorata mycelium extract;
D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain purple sesame health products.
Preferably, in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
Advantage of the present invention is: the present invention adopts that the purple sesame bacterium in bacterial classification original producton location separates, purifying and cultivation; Adopt industrialization liquid deep layer fermenting technology, make purple sesame bacterial classification to carry out a large amount of breedings in the liquid medium within, and; All contain in the metabolite of gained and the mycelium and the identical active ingredient of former purple sesame bacterium effectiveness; In addition, the present invention also makes the fructification of purple sesame bacterium to produce and to cultivate in industrialized condition, and technology is simple, cost is controlled; Thereby can reduce production costs significantly, make ordinary consumer also can use famous and precious health product.
The specific embodiment
Embodiment 1:
Following prepare the preferred embodiment of the method for purple sesame health products for the present invention is a kind of through liquid deep layer fermenting, preparation method of the present invention comprises following key step:
A, at first; Purple sesame bacterium is inserted in the nutrient solution; Nutrient solution of the present invention can be the various nutrient solutions that are suitable for purple sesame bacteria liquid cultivation of the prior art, and under 18 ℃ temperature, on shaking table, shakes cultivation 72 hours with 300 times/minute concussion frequency, obtains strain liquid; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 8% fructus hordei germinatus, weight portion 6% soybean, and inoculum concentration is 5%, and ventilation was cultivated 48 hours under 27 ℃ temperature; Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation; C, the more purple camphorata mycelium that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.8:5, extraction temperature is 95 ℃, extraction time is 2 hours, thereby obtains purple camphorata mycelium extract; Further, purple sesame zymotic fluid and wet thallus that the present invention mainly utilizes industrialization liquid deep layer fermenting technology to obtain prepare and produce purple sesame health products or health drink; D, last after the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other, concentrates 4 times with the mixed liquor of gained, and makes its refractive power pol reach 10%, thereby obtain purple sesame health products.Therefore,, popular straight drink product be can produce, health products and beverage comprised through preparation method of the present invention.And preparation method of the present invention can directly apply to suitability for industrialized production, and cost is low, and the products obtained therefrom trophic function is high, helps consumer and public daily health carings.
More specifically, preparation method of the present invention can directly carry out industrial and large-scale and produces continuously.After the batch process, can produce the purple sesame fermented liquid more than 10 tons every day, and produce 20 tons～final products more than 50 tons every day through preparation method of the present invention.
On the other hand, the method for concentration that in preparation method's of the present invention step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained purple sesame health products, the content of its functional components is close with purple sesame fructification or be higher than purple sesame fructification, and its effectiveness is with directly to take purple sesame suitable.
More preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) are preferably and reach relevant parameters separately simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed natural pH value of purple sesame fermented liquid and purple camphorata mycelium extract is 6～7, and the refractive power pol is 2～6%.
The content of functional component of the inventive method products therefrom and quality are all highly stable, and are higher than modern pharmacology and research and analyse the numerical value scope of determination to natural Cordyceps militaris component content.Preparation method's of the present invention industrialization control ability is strong, and its cost is far below natural material.
Embodiment 2:
Preparation method's of the present invention another kind of preferred embodiment will be provided as follows.The preparation method of present embodiment comprises: A, at first, select food, the purple sesame of medicinal bacterium for use, and purple sesame bacterium is inserted in the nutrient solution, and under 28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 96 hours with 200 times/minute concussion frequency; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 12% fructus hordei germinatus, weight portion 3% soybean; Inoculum concentration is 10%; And ventilation was cultivated 72 hours under 23 ℃ temperature, through biofermentation, obtained the purple camphorata mycelium and the purple sesame fermented liquid of fermentation; C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 1:2, extraction temperature is 90 ℃, extraction time is 3 hours, thereby obtains purple camphorata mycelium extract; D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 7 times, and makes its refractive power pol reach 12%, thereby obtain purple sesame health products.
Embodiment 3:
Another preferred embodiment of preparation method of the present invention will be provided as follows.The preparation method of present embodiment comprises the steps: A, purple sesame bacterium is inserted in the nutrient solution, and under 22 ℃ temperature, on shaking table, shakes cultivation 80 hours with 260 times/minute concussion frequency, obtains strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 10% fructus hordei germinatus, weight portion 5% soybean; Inoculum concentration is 7%; And ventilation was cultivated 60 hours under 25 ℃ temperature, through biofermentation, obtained the purple camphorata mycelium and the purple sesame fermented liquid of fermentation; C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.9:4, extraction temperature is 93 ℃, extraction time is 2.5 hours, thereby obtains purple camphorata mycelium extract; D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 6 times, and makes its refractive power pol reach 11%, thereby obtain purple sesame health products.
Embodiment 4:
Present embodiment provides a kind of purple sesame health products through the liquid deep layer fermenting preparation; Said purple sesame health products are to be prepared from following method: A, will select for use food, medicinal purple sesame bacterium to insert in the nutrient solution; And under 18～28 ℃ temperature (present embodiment is preferably 20 ℃), on shaking table, shake 72～96 hours (present embodiment is preferably 82 hours) of cultivation with the concussion frequency of 200～300 times/minute (present embodiment is preferably 260 times/minute), obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% (present embodiment is preferably 11%) fructus hordei germinatus, weight portion 3～6% (present embodiment is preferably 5%) soybean; Inoculum concentration is 5～10% (present embodiment is preferably 8%); And ventilation is cultivated 48～72 hours (present embodiment is preferably 56 hours under the temperature of 23～27 ℃ (present embodiment is preferably 26 ℃); Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation; C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after; Water extracts; The lixiviate ratio is 0.8～1:2～5 (present embodiment is preferably 1:3); Extraction temperature is 90～95 ℃ (present embodiment is preferably 92 ℃), and extraction time is 2.5 hours, thereby obtains purple camphorata mycelium extract; D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain purple sesame health products.
Wherein, the method for concentration that in step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained purple sesame health products, its effectiveness is with directly to take purple sesame suitable.
Preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) reach corresponding separately index simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed pH value of purple sesame fermented liquid and purple camphorata mycelium extract is 6～7, and the refractive power pol is 2～6%.
Other embodiment of a kind of purple sesame health products through liquid deep layer fermenting preparation of the present invention are identical or close with the foregoing description 1,2 and 3, repeat no more at this.To those skilled in the art, be not difficult from the foregoing description 1,2 and 3 to obtain relevant teachings, and in order to prepare or to produce purple sesame health products.
Obviously, those of ordinary skill in the art can use a kind of purple sesame health products that pass through the liquid deep layer fermenting preparation of the present invention and preparation method thereof, forms various types of health products and preparation method thereof.
The foregoing description only supplies to explain the present invention's usefulness; And be not to be limitation of the present invention; The those of ordinary skill in relevant technologies field without departing from the present invention, can also be made various variations and modification; Therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited each claim.
Claims (8)
1. prepare the method for purple sesame health products through liquid deep layer fermenting, it is characterized in that: said preparation method comprises the steps:
A, purple sesame bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation;
C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains purple camphorata mycelium extract;
D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain purple sesame health products.
2. according to claim 1ly prepare the method for purple sesame health products, it is characterized in that: in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration through liquid deep layer fermenting.
3. according to claim 2ly prepare the method for purple sesame health products through liquid deep layer fermenting, it is characterized in that: the film device operating pressure position of said reverse osmosis membrane method for concentration is set in 40kg/cm
2Below, and under room temperature, operate.
4. the method for preparing purple sesame health products through liquid deep layer fermenting according to claim 1; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
5. according to claim 4ly prepare the method for purple sesame health products through liquid deep layer fermenting, it is characterized in that: in said step D, the mutual mixed pH value of purple sesame fermented liquid and purple camphorata mycelium extract is 6～7, and the refractive power pol is 2～6%.
6. one kind is passed through the purple sesame health products that liquid deep layer fermenting prepares, and it is characterized in that: said purple sesame health products are to be prepared from following method:
A, purple sesame bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the purple camphorata mycelium and the purple sesame fermented liquid of fermentation;
C, the purple camphorata mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains purple camphorata mycelium extract;
D, the purple camphorata mycelium extract that obtains among purple sesame fermented liquid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is concentrated 4～7 times, and makes its refractive power pol reach 10～12%, thereby obtain purple sesame health products.
7. the purple sesame health products through the liquid deep layer fermenting preparation according to claim 6 is characterized in that: in said step D, utilize the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
8. the purple sesame health products through the liquid deep layer fermenting preparation according to claim 6; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4281021A (en) * | 1979-05-10 | 1981-07-28 | Noda Shokkin Kogyo Kabushiki Gaisha | Method of accelerating coloring and sweetening of citrus fruits |
| CN1076368A (en) * | 1993-02-20 | 1993-09-22 | 李汝清 | Compound glossy ganoderma nutrient solution and preparation method thereof |
| CN1507772A (en) * | 2002-12-16 | 2004-06-30 | 北京市食品研究所 | Method for preparing hickory chick by liquid deep fermentation and product thereof |
-
2012
- 2012-07-20 CN CN2012102549438A patent/CN102771779A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4281021A (en) * | 1979-05-10 | 1981-07-28 | Noda Shokkin Kogyo Kabushiki Gaisha | Method of accelerating coloring and sweetening of citrus fruits |
| CN1076368A (en) * | 1993-02-20 | 1993-09-22 | 李汝清 | Compound glossy ganoderma nutrient solution and preparation method thereof |
| CN1507772A (en) * | 2002-12-16 | 2004-06-30 | 北京市食品研究所 | Method for preparing hickory chick by liquid deep fermentation and product thereof |
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Application publication date: 20121114 |