CN102771496B - Plant growth regulator composition containing polyprenols and preparation method thereof - Google Patents
Plant growth regulator composition containing polyprenols and preparation method thereof Download PDFInfo
- Publication number
- CN102771496B CN102771496B CN201210265860.9A CN201210265860A CN102771496B CN 102771496 B CN102771496 B CN 102771496B CN 201210265860 A CN201210265860 A CN 201210265860A CN 102771496 B CN102771496 B CN 102771496B
- Authority
- CN
- China
- Prior art keywords
- polypentenol
- lipoid
- plant growth
- preparation
- urea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000000203 mixture Substances 0.000 title claims abstract description 18
- 229920001731 Polyprenol Polymers 0.000 title abstract description 20
- 229930186185 Polyprenol Natural products 0.000 title abstract description 20
- 150000003096 polyprenols Chemical class 0.000 title abstract description 20
- 238000002791 soaking Methods 0.000 claims abstract 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 30
- 239000004202 carbamide Substances 0.000 claims description 30
- 244000194101 Ginkgo biloba Species 0.000 claims description 22
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 22
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 241000196324 Embryophyta Species 0.000 claims description 19
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 239000000741 silica gel Substances 0.000 claims description 16
- 229910002027 silica gel Inorganic materials 0.000 claims description 16
- 239000000284 extract Substances 0.000 claims description 14
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 12
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 235000011201 Ginkgo Nutrition 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004952 Polyamide Substances 0.000 claims description 6
- 230000001476 alcoholic effect Effects 0.000 claims description 6
- 229960000892 attapulgite Drugs 0.000 claims description 6
- 239000013078 crystal Substances 0.000 claims description 6
- 239000000945 filler Substances 0.000 claims description 6
- 229910052625 palygorskite Inorganic materials 0.000 claims description 6
- 229920002647 polyamide Polymers 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 239000006184 cosolvent Substances 0.000 claims description 5
- 239000003995 emulsifying agent Substances 0.000 claims description 5
- 239000003381 stabilizer Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 4
- 238000010828 elution Methods 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 4
- 240000000249 Morus alba Species 0.000 claims description 3
- 235000008708 Morus alba Nutrition 0.000 claims description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 3
- 235000011613 Pinus brutia Nutrition 0.000 claims description 3
- 241000018646 Pinus brutia Species 0.000 claims description 3
- ZNUAKACHFYTNFX-UHFFFAOYSA-N Wallichenol Natural products CC12CCC(O)C(C)(C)C1CCC1(C)C2CCC2C3C(C(=C)CO)CCC3(C)CCC21C ZNUAKACHFYTNFX-UHFFFAOYSA-N 0.000 claims description 3
- FVWJYYTZTCVBKE-ROUWMTJPSA-N betulin Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(CO)CC[C@@H](C(=C)C)[C@@H]5[C@H]4CC[C@@H]3[C@]21C FVWJYYTZTCVBKE-ROUWMTJPSA-N 0.000 claims description 3
- MVIRREHRVZLANQ-UHFFFAOYSA-N betulin Natural products CC(=O)OC1CCC2(C)C(CCC3(C)C2CC=C4C5C(CCC5(CO)CCC34C)C(=C)C)C1(C)C MVIRREHRVZLANQ-UHFFFAOYSA-N 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 3
- 230000008025 crystallization Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 238000004945 emulsification Methods 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 239000002674 ointment Substances 0.000 claims 3
- 239000012141 concentrate Substances 0.000 claims 2
- CMXPERZAMAQXSF-UHFFFAOYSA-M sodium;1,4-bis(2-ethylhexoxy)-1,4-dioxobutane-2-sulfonate;1,8-dihydroxyanthracene-9,10-dione Chemical compound [Na+].O=C1C2=CC=CC(O)=C2C(=O)C2=C1C=CC=C2O.CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC CMXPERZAMAQXSF-UHFFFAOYSA-M 0.000 claims 2
- 239000002904 solvent Substances 0.000 claims 2
- 239000006004 Quartz sand Substances 0.000 claims 1
- 238000000874 microwave-assisted extraction Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 9
- 230000003203 everyday effect Effects 0.000 abstract description 6
- 230000012010 growth Effects 0.000 abstract description 6
- 230000007226 seed germination Effects 0.000 abstract description 6
- 230000008635 plant growth Effects 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 241001464837 Viridiplantae Species 0.000 abstract description 2
- 230000002262 irrigation Effects 0.000 abstract 1
- 238000003973 irrigation Methods 0.000 abstract 1
- 230000007246 mechanism Effects 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 description 14
- 241000209140 Triticum Species 0.000 description 11
- 235000021307 Triticum Nutrition 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 230000035784 germination Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 238000011010 flushing procedure Methods 0.000 description 8
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 239000013641 positive control Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000000926 separation method Methods 0.000 description 5
- 238000012549 training Methods 0.000 description 5
- 241001423497 Himalayacalamus falconeri Species 0.000 description 4
- 244000178231 Rosmarinus officinalis Species 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 4
- 235000007516 Chrysanthemum Nutrition 0.000 description 3
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 3
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 3
- 244000088415 Raphanus sativus Species 0.000 description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 208000006454 hepatitis Diseases 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- YHQXBTXEYZIYOV-UHFFFAOYSA-N 3-methylbut-1-ene Chemical group CC(C)C=C YHQXBTXEYZIYOV-UHFFFAOYSA-N 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 240000002024 Gossypium herbaceum Species 0.000 description 2
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- OSASVXMJTNOKOY-UHFFFAOYSA-N chlorobutanol Chemical compound CC(C)(O)C(Cl)(Cl)Cl OSASVXMJTNOKOY-UHFFFAOYSA-N 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000007127 saponification reaction Methods 0.000 description 2
- 238000010898 silica gel chromatography Methods 0.000 description 2
- -1 terpene alcohols Chemical class 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 1
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 description 1
- TXKJNHBRVLCYFX-UHFFFAOYSA-N (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2,6,10,14,18,22,26,30,34,38,42-undecaen-1-ol Natural products CC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCO TXKJNHBRVLCYFX-UHFFFAOYSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 208000035126 Facies Diseases 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- TXKJNHBRVLCYFX-RDQGWRCRSA-N all-trans-undecaprenol Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CO TXKJNHBRVLCYFX-RDQGWRCRSA-N 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960004926 chlorobutanol Drugs 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 229920002672 di-trans,poly-cis-Undecaprenol Polymers 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 150000004141 diterpene derivatives Chemical class 0.000 description 1
- 150000002031 dolichols Chemical group 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- ZKQFHRVKCYFVCN-UHFFFAOYSA-N ethoxyethane;hexane Chemical compound CCOCC.CCCCCC ZKQFHRVKCYFVCN-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
- KDCIHNCMPUBDKT-UHFFFAOYSA-N hexane;propan-2-one Chemical compound CC(C)=O.CCCCCC KDCIHNCMPUBDKT-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- NIQQIJXGUZVEBB-UHFFFAOYSA-N methanol;propan-2-one Chemical compound OC.CC(C)=O NIQQIJXGUZVEBB-UHFFFAOYSA-N 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920001550 polyprenyl Polymers 0.000 description 1
- 125000001185 polyprenyl group Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- OVYTZAASVAZITK-UHFFFAOYSA-M sodium;ethanol;hydroxide Chemical compound [OH-].[Na+].CCO OVYTZAASVAZITK-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a plant growth regulator composition containing polyprenols and a preparation method thereof and belongs to the field of development and application of plant growth regulatory substance. The preparation method comprises the step of using polyprenols with 0.1-100mg/L of effective concentration to perform soaking, sprinkling and other treatment on irrigation and plants, plant tissues and seeds for1-3 times every day, wherein the treatment lasts for 1-40 days, and seed germination, seedling growth and striking of the plant tissue roots can be promoted. Due to the fact that the polyprenols is chemical substance from natural plants and is free of toxicity to relevant metabolic mechanisms in human body and the plants and free of harm to the environment, the fact that polyprenols is used for developing natural green plant growth regulators has important significance and has good business prospect.
Description
Technical field
The present invention relates to a kind of content has plant growth regulator composition of polypentenol and preparation method thereof, belongs to the development and application field of plant growth regulating substance, is exclusively used in the fields such as agricultural chemicals exploitation, farmland chemical regulation and provenance agricultural.
Technical background
Polypentenol (polyprenols) is extensively present in the plants such as green confierophyte and ginkgo, be to take C5 isopentene group as the single lipids of structure, structure, can divide: the n-OH of 3-(cis) n-OH of 2-(cis) n-OH of betulinol type ω-(trans), luxuriant and rich with fragrance card alcohol type ω-(trans) and alltrans Salanesol type ω-(trans).
Polypentenol and ester class thereof are nonpolar ester type compounds, its leaching process is: the separation of extraction-hydrolysis-extraction-column chromatography or molecular clock etc., main organic solvent has: chloroform, n-hexane, benzinum, toluene, benzene, pentane etc., can also use mixed solvent, as: n-hexane-ether, acetone-chloroform, methanol-acetone, n-hexane-acetone.The employing benzinums such as Yang little Ming carry out lixiviate to ginkgo leaf, obtain liposoluble substance, further after hydrolysis, adopt n-hexane to extract, by saturated sodium-chloride water solution washing organic facies, obtain polypentenol and oil-soluble impurities, with thin layer chromatography, carry out separation, and then adopt column chromatography purification.Li Chunbin etc. dissolve ginkgo leaf ligroin extraction with acetone, carry out purification by silica gel column chromatography after filtration, with benzinum and benzinum: ether (25: 1) linear gradient elution, the polyprenyl acetates purity obtaining reaches more than 90%.The employing benzinums such as Sun Lei extract with the solid-liquid ratio of 1: 7, and 10 hours, the extract obtained column chromatography for separation of carrying out, obtained polypentenol.The employing benzinums such as Wang Chengzhang carry out lixiviate to ginkgo leaf, with EtOH-NaOH solution, it is refluxed, then with saponification agent by preparing unsaponifiable matter at 1: 5.Deng Yongzhi etc. carry out carbon dioxide supercritical extraction to ginkgo leaf, and then adopt benzinum and ether to dissolve in the ratio of 19: 1, with silica gel column chromatography, carry out separation afterwards, with the centrifugal removal of impurities of acetone solution eluate with obtain the polypentenol of purifying.
The process of growing of plant is subject to the regulation and control of plant endogenous hormones conventionally, is also subject to the restriction of various unsuitable environmental conditions simultaneously, often causes the decline of growth rate and output, and the metabolic function downward at each tissue of plant and position is one of main inducing wherein.Plant growth regulator is the chemicals of natural source, for example, also referred to as plant hormone (growth hormone, gibberellin, cytokinins, ethene, brassinosteroid or abscisic acid), fat oligosaccharides, peptide, derivative of fatty acid and oligosaccharins, or they can be the synthetic compounds (the derivative biology of natural generation auxin, ethrel) making.Plant growth regulator is the trace level chemical substance that coordinate plant growth is grown, and growth and development of plants needs the supply of liquid manure nutrition, is also subject to adjusting and the control of plant growth substance simultaneously.At low dense lower energy, produce the physiological effect of obviously showing, make plant that strong Physiology and biochemistry occur and react with form.Plant, at rooting of cuttings, promotes bolting to bloom, solid, prevent from coming off, and vegetables fruit thinning, induction and breaking dormancy, promote ripe and strengthen the aspects such as resistance, applied widely.From eighties of last century nineties, the research and development of natural source plant growth regulator become one of focus of domestic research.Utilize natural plants as the raw material that extracts plant growth regulator, realize agriculture sustainable development significant.
At present, both at home and abroad the research of natural polypentenol is mainly concentrated on to anti-hepatitis, the biologically active such as antitumor.Research shows that polyprenols is natural products, nontoxic to human body, without mutagenesis, without teratogenesis with without carcinogenesis, at aspects such as hepatitis virus resisting and AIDS virus and NACT leukemia, there is obvious biologically active, to immunologic function diseases such as hypertension, high fat of blood, diabetes, gout, lupus erythematosus, chronic hepatitis, suppressing tumour and auxiliaryization, radiotherapy all has obvious effect.Polypentenol has good inhibition to mouse transplantability hepatoma Heps, sarcoma s180 and Emhorn knurl EC.But polypentenol should be made in to plant growth regulating almost not to be had.Korea S power is held dragon and from cotton plant leaf, is extracted separation and obtain undecaprenol and ten diterpene alcohol, study it and be applied to plant seed as plant growth regulator, result shows, its concentration has good stratification effect [Chinese patent CN1529550A] to seeds such as corn, cucumber when 100ppm and 50ppm.And polypentenol in this patent is the birch terpene alcohols polypentenol by 14~24 (take 17~19 as main) individual isopentene group cell formation, in its chemical constitution and cotton plant leaf, extract obtain different.Polypentenol is the compound in natural plants source, has safety, easily biological-degradable, the feature such as environmentally friendly, therefore utilizes polypentenol exploitation natural green plant growth regulator to have important practical significance.
Summary of the invention
For achieving the above object, having invented a kind of content has plant growth regulator composition of polypentenol and preparation method thereof, it is characterized in that being comprised of following steps:
(1) preparation of polypentenol: by the extraction of plant leaf powder process, saponification, washing, after being dried;
(2) preparation of urea alcoholic solution: urea is put into absolute methanol, bathe and to be heated to urea and all to dissolve filling nitrogen Water Under, wherein the w/v of urea and absolute methanol is 1: 10~5: 10, and heating reflux temperature is 60~75 ℃;
(3) carry out urea clathrate reaction: in urea alcoholic solution, add polypentenol crude extract and filler, polypentenol crude extract: filler: the ratio 0.5~1: 1~2: 10~40 of urea alcoholic solution, stirring and refluxing at 60-75 ℃ of temperature, be cooled to after room temperature, at-20~10 ℃ of deepfreezes to urea inclusion crystallization, then suction filtration, collects respectively crystal and filtrate.
(4) crystal step (3) being obtained fills post, at the uniform velocity injects chromatographic column carry out wash-out with mobile phase, collects elution fraction, merge, the polypentenol after being refined after concentrating, analyzes through HPLC, the purity of sample is more than 50%, and the rate of recovery is more than 80%.
(5) polypentenol step (4) being obtained, by percentage, be: polypentenol 1%~20%, cosolvent 1~50%, emulsifier 1~20%, stabilizing agent 1~10%, water surplus, at room temperature ultrasonic filter emulsification 10~30min, preserves at low temperature below 4 ℃, before using, by deionized water, is diluted to desired concn, stir, make the plant growth regulator composition that content has polypentenol.
2, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that plant leaf in step (1) is a kind of in ginkgo leaf, pine needle, mulberry leaf, the structure of its polypentenol is betulinol type ω-(trans)
2-(cis)
n-OH, wherein the scope of n is 10~20.
3, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that the filler in step (3) is one or more in silica gel, active carbon, polyamide, attapulgite, aluminium oxide, diatomite.
4, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that mobile phase in step (4) is a kind of in benzinum, n-hexane, ether.
5, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that the cosolvent in step (5) selects one or more in methyl alcohol, ethanol, acetone, emulsifier selects one or more in Tween 80, OP-10, OP-21, X-100, and stabilizing agent is selected from one or more in n-butanol, propyl alcohol, glycerine.
6, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, and it is characterized in that being mixed with polypentenol after solution is 0.1~100mg/L as effective final concentration of plant growth regulator.
7, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that, plant growth regulator to plant, plant tissue or seed irrigate, spray wine, immersion or
process, or directly add the medium that comprises above-mentioned vegetable material, process continuously every day 1~3 time 1~40 day.
8, a kind of content has plant growth regulator composition of polypentenol and preparation method thereof according to claim 1, it is characterized in that, described plant or seed are a kind of in wheat, ternip, Yellow Emperor, a legendary ruler chrysanthemum, cucumber, fairy bamboo and rosemary, Xue MingRosma rinus officinalis.
A kind of content provided by the present invention has plant growth regulator composition of polypentenol and preparation method thereof, also has the following advantages:
1) pollution-free, environmental protection polypentenol derives from ginkgo leaf, pine needle, mulberry leaves etc., easily biological-degradable, dolichol structure contained in it and human body and mammal internal organs approaches, and can in human body, be metabolized to dolichol phosphate ester, as the biosynthetic carrier of glycoprotein.Therefore under the various practice conditions such as farmland and greenhouse, use low concentration polypentenol can not cause adverse effect to ecotope.
2) applied range this method is exclusively used in nuisanceless production, the field such as group training etc. that comprises agricultural chemicals exploitation, farmland chemical regulation, provenance agricultural, can regulating plant seed germination, take root, the growth of crop and controlling through saving the effects such as growth.
Embodiment
Following examples are more of the present invention giving an example, and should not be seen as limitation of the invention.
Embodiment 1: urea adduct method is prepared the method for polypentenol
Select four kinds of different types of absorption carriers such as silica gel, active carbon, polyamide, attapulgite, studied respectively the purifying research of 5 kinds of different quality proportionings such as urea and absorption carrier 10: 1 in mass ratio, 5: 1,5: 2,5: 3,1: 1.
The purification result of the different proportionings of table 1 urea silica gel
Take silica gel as shown in Table 1 as carrier, benzinum is that eluant, eluent can find out that by the experimental result of the mass ratio of different urea and silica gel polypentenol yield under pure urea condition reaches 92.08%, but its purity is only 39.83%, it is the highest that urea and silica gel mass ratio are that prepare at 5: 1 fixing loads the Polyprenols From Ginkgo Biloba L purity that purifying obtains mutually, its purity is 72.34%, along with its purity of increase of silica gel consumption diminishes again gradually, yield also declines thereupon gradually.Consider the factor of its purity and yield, the best purification condition that the silica gel of take is carrier as urea silica gel mass ratio be 5: 1, its purity is 72.34%, compared with the purity of molecular clock product C 37.9%, is greatly improved.It is irregular that pure urea loads urea crystal in crystallization process, and the inhomogeneous purification effect of dress post is poor; Along with the consumption of silica gel increases urea in Silica Surface load, product purity improves gradually, is issued to the maximum of purity 5: 1 conditions; Along with the purity of the decline polypentenol of urea ratio reduces gradually; Increase with the consumption of silica gel strengthens and causes yield to decline gradually the suction-operated of polypentenol.
The purification result of table 2 different carriers
As shown in Table 2, silica gel, active carbon, attapulgite and four kinds of different absorption carriers of polyamide have been selected, mass ratio between urea and carrier 5: 1, by contrast experiment find take silica gel as the product purity that carrier obtains higher, active carbon takes second place, and polyamide and attapulgite are that the product purity that carrier obtains improves little.The more aobvious brownish red of the product colour that polyamide, attapulgite purifying obtain, silica gel takes second place, and product colour that activated carbon purification obtains is thin out is buff.Consider and take silica gel as carrier the best.
Embodiment 2: the preparation of Polyprenols From Ginkgo Biloba L emulsion
With embodiment 1, extract the separated Polyprenols From Ginkgo Biloba L obtaining, by Polyprenols From Ginkgo Biloba L 5g, tween 2.5g, ethanol 2.5mL and water 15mL, in ultrasonic disruption instrument, carry out ultrasonic homogeneous, obtain polypentenol emulsion, and take it as mother liquor, be mixed with 5 concentration (10mg/L, 20mg/L, 50mg/L and 100mg/L).
Embodiment 3: the impact that crop seeds is sprouted
3-1: the impact on wheat seeds sprouting and growth of seedling
Selected in the same size, the wheat seed of full seed, in ultra-clean aseptic working platform, the NaClO solution with 5% soaks 20min, with distilled water flushing for several times.Under aseptic condition, take out, proceed in polypentenol sample, with distilled water, make blank, using 20mg/L IAA (heteroauxin) as positive control, each is treated to 100 seeds, establishes 3 repetitions; Seed soaks after 4h, uses distilled water flushing the surface of the seed, and seed is fitly arranged in and is placed in the auxiliary glass training shape ware that has 2 layers of gauze, puts into illumination box, under 25 ℃ of conditions, cultivates 7 days.Duration of germination is observed every day, keeps the skin wet, and a record germination grain number, height of seedling and the root of its seedling are long, survey its amylase activity and improving activity of root system.The results are shown in table 3-8.
The comparison of table 3 Polyprenols From Ginkgo Biloba L to wheat seed germinating rate
The comparison of table 4 Polyprenols From Ginkgo Biloba L to wheat seed height of seedling
The comparison that table 5 Polyprenols From Ginkgo Biloba L is long to wheat seed root
The comparison of table 6 Polyprenols From Ginkgo Biloba L to wheat seed amylase activity
The comparison of table 7 ginkgo polypentenol to wheat seed improving activity of root system
3-2: the impact on Yellow Emperor, a legendary ruler chrysanthemum seed germination
Selected in the same size, the wheat seed of full seed, in ultra-clean aseptic working platform, the NaClO solution with 5% soaks 20min, with distilled water flushing for several times.Under aseptic condition, take out, proceed in polypentenol sample, with distilled water, make blank, using 20mg/L IAA (heteroauxin) as positive control, each is treated to 100 seeds, establishes 3 repetitions; Seed soaks after 4h, uses distilled water flushing the surface of the seed, and seed is fitly arranged in and is placed in the auxiliary glass training shape ware that has 2 layers of gauze, puts into illumination box, under 25 ℃ of conditions, cultivates 10 days.Duration of germination is observed every day, keeps the skin wet, and a record germination grain number.The results are shown in Table 8.
The comparison of table 8 Polyprenols From Ginkgo Biloba L to Yellow Emperor, a legendary ruler chrysanthemum percentage of seedgermination
3-3: the impact that dialogue radish seed is sprouted
Selected in the same size, the wheat seed of full seed, in ultra-clean aseptic working platform, the NaClO solution with 5% soaks 20min, with distilled water flushing for several times.Under aseptic condition, take out, proceed in polypentenol sample, with distilled water, make blank, using 20mg/L IAA (heteroauxin) as positive control, each is treated to 100 seeds, establishes 3 repetitions; Seed soaks after 4h, uses distilled water flushing the surface of the seed, and seed is fitly arranged in and is placed in the auxiliary glass training shape ware that has 2 layers of gauze, puts into illumination box, under 25 ℃ of conditions, cultivates 5 days.Duration of germination is observed every day, keeps the skin wet, and a record germination grain number.The results are shown in Table 9.
The comparison of table 9 Polyprenols From Ginkgo Biloba L dialogue radish seed germination rate
3-4: the impact on germination of cucumber seeds
Selected in the same size, the wheat seed of full seed, in ultra-clean aseptic working platform, the NaClO solution with 5% soaks 20min, with distilled water flushing for several times.Under aseptic condition, take out, proceed in polypentenol sample, with distilled water, make blank, using 20mg/L IAA (heteroauxin) as positive control, each is treated to 100 seeds, establishes 3 repetitions; Seed soaks after 4h, uses distilled water flushing the surface of the seed, and seed is fitly arranged in and is placed in the auxiliary glass training shape ware that has 2 layers of gauze, puts into illumination box, under 25 ℃ of conditions, cultivates 5 days.Duration of germination is observed every day, keeps the skin wet, and a record germination grain number.The results are shown in Table 10.
The comparison of table 10 Polyprenols From Ginkgo Biloba L dialogue radish seed germination rate
Embodiment 4: the impact on plant cuttage root-taking
4-1: the impact on fairy bamboo cuttage root-taking
Selected fairy bamboo branch of the same size takes out under aseptic condition, proceeds in polypentenol sample, with distilled water, makes blank, usings 20mg/L IAA (heteroauxin) as positive control, and each is treated to 30, establishes 3 repetitions; Soak after 2h, be placed in test tube and cultivate, under 25 ℃ of conditions, cultivate 15 days, record the number of taking root.In the results list 11.
The impact of table 11 Polyprenols From Ginkgo Biloba L on fairy bamboo cuttage root-taking
4-2: the impact on rosemary, Xue MingRosma rinus officinalis cuttage root-taking
Selected rosemary, Xue MingRosma rinus officinalis branch of the same size takes out under aseptic condition, proceeds in polypentenol sample, with distilled water, makes blank, usings 20mg/L IAA (heteroauxin) as positive control, and each is treated to 30, establishes 3 repetitions; Soak after 2h, be placed in test tube and cultivate, under 25 ℃ of conditions, cultivate 15 days, record the number of taking root.In the results list 12.
The impact of table 12 Polyprenols From Ginkgo Biloba L on rosemary, Xue MingRosma rinus officinalis cuttage root-taking
Claims (5)
1. a preparation method who contains the plant growth regulator of polypentenol lipoid, is characterized in that being comprised of following steps:
(1) polypentenol lipoid extracts: will be rich in the fresh plant leaf of polypentenol lipoid, room temperature is dried in the shade, be crushed to the following powder of 100 order, add any solvent in anhydrous propanone or C1~C3 alcohol, leaf and solvent are 1: 20 in mass ratio, soaking at room temperature 10~72 hours, or extract 10~60 minutes through Microwave Extraction or ultrasonic at 10 ℃~80 ℃, repeat to extract twice, merge extract, vacuum reclaims organic solvent, and concentrate is polypentenol class fat ointment;
(2) polypentenol lipoid extract: it is 1: 6~30 anhydrous propanones that polypentenol class fat ointment adds mass ratio, and room temperature is dissolved as solution; 40~200 order diatomite mix with quartz sand 1: 1 in mass ratio~10, obtain mixed aid, mixed aid is 0.5~20 with polypentenol class fat ointment mass ratio: 100 mix, at-30 ℃~0 ℃, stir 2~30 hours, 3000~20000 revs/min of centrifuges filter, clear liquid reclaims organic solvent, and concentrate is polypentenol lipoid extract;
(3) preparation of urea alcoholic solution: urea is put into absolute methanol, bathe and to be heated to urea and all to dissolve filling nitrogen Water Under, wherein the w/v of urea and absolute methanol is 1: 1~10, and heating reflux temperature is 40~90 ℃;
(4) polypentenol lipoid extract urea inclusion preparation: add polypentenol lipoid extract and filler in urea alcoholic solution, polypentenol lipoid extract: filler: the mass ratio 0.1~1: 1~3: 10~40 of urea alcoholic solution, stirring and refluxing at 60-75 ℃ of temperature, be cooled to after room temperature, at-20~10 ℃ of deepfreezes to urea inclusion crystallization, then suction filtration, collects respectively crystal and filtrate, and crystal is polypentenol lipoid extract urea inclusion;
(5) polypentenol lipoid purifying: by polypentenol lipoid urea clathrate crystal dress post, with non-polar solven, carry out wash-out, elution volume is column volume 1-4 times, TLC detects, merge polypentenol lipoid elution fraction, after concentrating, obtain the polypentenol lipoid of purifying, through HPLC, detect, polypentenol lipoid purity is more than 50%, and the rate of recovery is more than 75%;
(6) polypentenol lipoid step (5) being obtained, mix by mass percentage with cosolvent, emulsifier and stabilizing agent and water, polypentenol lipoid 1%~20% in 100 parts of quality, cosolvent 1~50%, emulsifier 1~20%, stabilizing agent 1~10%, water surplus, at room temperature ultrasonic emulsification 10~30min, stirs, at low temperature below 4 ℃, preserve, make the plant growth regulator composition that contains polypentenol lipoid.
2. a kind of preparation method of the plant growth regulator that contains polypentenol lipoid according to claim 1, it is characterized in that plant leaf in step (1) is a kind of in ginkgo leaf, pine needle, mulberry leaf, the structure of its polypentenol lipoid is betulinol type ω-(trans)
2-(cis)
n-OH, wherein the scope of n is 10~20.
3. a kind of preparation method of the plant growth regulator that contains polypentenol lipoid according to claim 1, is characterized in that the filler in step (4) is one or more in silica gel, active carbon, polyamide, attapulgite, aluminium oxide, diatomite.
4. a kind of preparation method of the plant growth regulator that contains polypentenol lipoid according to claim 1, is characterized in that non-polar solven in step (5) is any in benzinum, n-hexane, ether.
5. a kind of preparation method of the plant growth regulator that contains polypentenol lipoid according to claim 1, it is characterized in that the cosolvent in step (6) is selected from one or more in methyl alcohol, ethanol, acetone, emulsifier is selected from one or more in Tween 80, OP-10, OP-21, X-100, and stabilizing agent is selected from one or more in n-butanol, propyl alcohol, glycerine.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210265860.9A CN102771496B (en) | 2012-07-26 | 2012-07-26 | Plant growth regulator composition containing polyprenols and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210265860.9A CN102771496B (en) | 2012-07-26 | 2012-07-26 | Plant growth regulator composition containing polyprenols and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102771496A CN102771496A (en) | 2012-11-14 |
CN102771496B true CN102771496B (en) | 2014-04-09 |
Family
ID=47116813
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210265860.9A Expired - Fee Related CN102771496B (en) | 2012-07-26 | 2012-07-26 | Plant growth regulator composition containing polyprenols and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102771496B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103145528B (en) * | 2013-01-24 | 2015-02-18 | 中国林业科学研究院林产化学工业研究所 | Method of preparing high-purity polyprenol lipid by means of both high-speed countercurrent chromatography and high performance liquid chromatography |
CN106857695A (en) * | 2016-12-31 | 2017-06-20 | 新昌县迪斯曼科技有限公司 | Plant growth regulator composition and preparation method thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1392167A (en) * | 2001-06-18 | 2003-01-22 | 中国林业科学研究院林产化学工业研究所 | Preparing process and use of ginkgo leaf polypentenol and ginkgo leaf extract |
CN1529550A (en) * | 2001-03-14 | 2004-09-15 | Plant growth regulator for increasing crop gield comprising polyprenol and extraction method thereof | |
CN101967083A (en) * | 2010-09-20 | 2011-02-09 | 中国科学院山西煤炭化学研究所 | Method for separating and refining polyprenol in ginkgo biloba extract |
-
2012
- 2012-07-26 CN CN201210265860.9A patent/CN102771496B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1529550A (en) * | 2001-03-14 | 2004-09-15 | Plant growth regulator for increasing crop gield comprising polyprenol and extraction method thereof | |
CN1392167A (en) * | 2001-06-18 | 2003-01-22 | 中国林业科学研究院林产化学工业研究所 | Preparing process and use of ginkgo leaf polypentenol and ginkgo leaf extract |
CN101967083A (en) * | 2010-09-20 | 2011-02-09 | 中国科学院山西煤炭化学研究所 | Method for separating and refining polyprenol in ginkgo biloba extract |
Non-Patent Citations (4)
Title |
---|
周春华等.银杏叶聚戊烯醇研究进展.《中成药》.2009,第31卷(第9期),1416-1419. |
李春斌等.银杏叶聚戊烯醇分离分析方法的研究进展.《大连民族学院学报》.2007,(第1期),第41-43页. |
银杏叶聚戊烯醇分离分析方法的研究进展;李春斌等;《大连民族学院学报》;20070115(第1期);第41-43页 * |
银杏叶聚戊烯醇研究进展;周春华等;《中成药》;20090930;第31卷(第9期);第1416-1419页 * |
Also Published As
Publication number | Publication date |
---|---|
CN102771496A (en) | 2012-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106386185A (en) | High-yield early-fruiting cultivation method for mulberry | |
CN104472486B (en) | Pear tree multifunctional growth regulator composition and using method | |
CN105359796A (en) | Middle maturing cantaloupe plantation method in northern part of Anhui province | |
CN106034928A (en) | Se-rich Averrhoa carambola planting method | |
CN105085093A (en) | Inorganic-organic compound fertilizer dedicated for camellia oleifera and preparation method thereof | |
CN105613015A (en) | Planting method of selenium-rich peony | |
CN107027611A (en) | It is a kind of to improve the roxburgh anoectochilus terminal bud tissue-cultured seedling speed of growth and the method for active component content | |
CN102771496B (en) | Plant growth regulator composition containing polyprenols and preparation method thereof | |
CN108496668A (en) | A kind of method for culturing seedlings of clausenae Lansium | |
CN111109310A (en) | Multi-effect pesticide composition and application thereof | |
CN105819996A (en) | Phytohormone-containing slow-release fertilizer for peanuts and preparation method thereof | |
CN103141251B (en) | Application of the brassinolide on capsicum | |
CN108076943A (en) | A kind of implantation methods of green fruit of selenium-rich | |
CN107182666A (en) | A kind of cultural method for improving thin-shelled walnut disease resistance | |
CN104381075A (en) | Rapid seeding and strong seedling cultivation method for apricots | |
CN114403147B (en) | Application of 1-methylpyrrolidine-2-carboxylic acid in promoting plant growth | |
CN105850466A (en) | Soybean planting method capable of increasing yield through plant hormone | |
AU2021105219A4 (en) | A fertilization method for gold sophora japonica l yield improvement | |
CN114126409A (en) | Application of chlorophyll chlorin as plant growth regulator | |
CN113892484B (en) | Application of alpha-pinene in promoting growth and inducing resistance of panax notoginseng | |
Imandi et al. | Studies on the effect of plant growth regulators on vegetative growth, flowering, yield and shelf life of the marigold Cv. Siracole | |
KR20040103146A (en) | Cultivating process of Gombo-Baechu | |
CN107637463A (en) | A kind of implantation methods of selenium-rich chrysanthemum | |
CN107114415A (en) | Matching used conditioning agent and growth accelerator when Chinese yew is interplanted in vineyard | |
CN110117205B (en) | Germanium-rich nutrient solution for increasing germanium content of rapeseeds and preparation method and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 |
|
CF01 | Termination of patent right due to non-payment of annual fee |