CN102771311A - Shiitake cultivation method - Google Patents
Shiitake cultivation method Download PDFInfo
- Publication number
- CN102771311A CN102771311A CN2012102696935A CN201210269693A CN102771311A CN 102771311 A CN102771311 A CN 102771311A CN 2012102696935 A CN2012102696935 A CN 2012102696935A CN 201210269693 A CN201210269693 A CN 201210269693A CN 102771311 A CN102771311 A CN 102771311A
- Authority
- CN
- China
- Prior art keywords
- mushroom
- cultivated
- juggle
- shiitake
- bacteria strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to the field of shiitake cultivation and particularly relates to a shiitake cultivation method, which comprises the following steps of: selecting broadleaf branches with diameter being 8-12mm, cutting the broadleaf branches into wood sections with length being 2-3cm, conducting sterilization treatment and putting the branches in sterile culture bags; inoculating shiitake stock culture on the treated and cooled wood sections, culturing at 20-25DEG C till the wood sections are overgrown with mycelia to obtain particle strains; and putting the obtained particle strains in culture bags, statically placing the culture bags at shady and cool places for 55-65 days and finally picking shiitake. The shiitake cultivation method has the advantages of simplicity in operation and high shiitake yield.
Description
Technical field
The invention belongs to agricultural planting, particularly the cultivating method of mushroom.
Background technology
Mushroom has another name called mushroom, belongs to basidiomycetes, Agaricales, Pleurotaceae, Lentinus.Mushroom is made up of mycelium and fruit body two parts.Mycelia is formed by spore germination, and white, fine hair shape have tabula and branch, and cell wall is thin, and very thin mycelia mutually combines, and constantly growth and breeding assembles mycelium.Mycelial growth is grown certain phase, sits the surface in matrix and forms fruit body---mushroom.The integral body of mushroom is formed by mycelia.During separate tissue, any part that cuts mushroom can grow new mycelia.The fruit body of mushroom is made up of cap, lamella, stem etc.Cap is circular, common 3～6 centimetres of diameter; Big individuality can reach more than 10 centimetres; The lid edge is just involute, and the back is flat to be sent out; Lid table brown or pitchy often have light scale.Bacterial context is plump, and the middle part can reach about 1 centimetre, and is soft and flexible, white.Give birth in the stem or wilfully, circular columns or flat slightly, white, meat real, grows 3～10 centimetres, 0.5～1 centimetre of diameter.Lamella white, dense and soft is gone out by the radiation of stem place, is blade-like, is the place that produces full son; Spore is white, smooth, oval.A mushroom can distribute tens spores.
Be to begin from spore germination the complete history of life of mushroom, finishes to forming full son again.In this process, sexual reproduction and asexual life are planted organically and are combined, and accomplish their history of life jointly.After the spore maturation of mushroom, the temperature that obtains suiting, wet condition will be sprouted and generate mycelia.Mycelia by full sub-germination and growth is called uninucleate hyphae.Uninucleate hyphae is grown in any case, also can not grow fruit body.After having only uninucleate hyphae when different sexes to combine to form nucleated mycelium, portion spreads breeding and forms mycelium in the substrate.Mycelium has accumulated sufficient nutriment, and has reached physiological ripening through certain growth and development stage, under optimum conditions, could form fruit body primordium, and constantly growth increases to the mushroom flower bud, fruit body was bred down with generation.The history of life of mushroom, divide two stages, one is the mycelium stage, one is the fruit body stage.Mushroom is the rotten bacterium of a kind of water.Do not have chlorophyll in the body, can not carry out photosynthesis, but rely on decomposition and absorption timber or other intramatrical nutrition to make a living.Contain the complete nutrients matter (carbon source, nitrogenous source, mineral matter and vitamin etc.) that mushroom growth is grown in the timber.Mushroom has and decomposes lignin, cellulosic ability in the timber, can be glucose, amino acid etc. with its decomposition and inversion, the nutriment that directly absorbs and utilize as hyphal cell.
The reproductive condition of mushroom interacts, interrelates.To the fruit body forming process, temperature is that high earlier back is low from mycelial growth, and humidity is to do afterwards earlier to wet, and light is that dark earlier back is bright.These conditions both connected each other, and mutual restriction must be considered comprehensively again, in order to avoid attend to one thing and lose sight of another, just can get a desired effect.
Summary of the invention
One of the object of the invention is to provide the method for mushroom cultivation, and this method stability is good, and yield is high.
For realizing above-mentioned technical purpose, technical scheme of the present invention is:
The method that mushroom is cultivated has by following steps and forms:
The preliminary treatment of A material
Choosing diameter is the broad-leaved branch of 8-12 millimeter, and the juggle that is cut into 2-3 centimetre is put into the aseptic culture bag after sterilization treatment;
The preparation of B granular bacteria strain
Mother's kind of mushroom is received on the juggle of processing of step A and cooling, under 20-25 ℃ of condition, cultivated to mycelia and cover with juggle, get granular bacteria strain;
The C enlarged culture
Step B gained granular bacteria strain is put into culture bag, and left standstill 55-65 days at shady and cool place, wins mushroom.
Further, the juggle in the steps A uses frequency to be 40-60kHz ultrasonic Treatment 15-30 minute before the sterilization.
Further, in the steps A, the method for sterilization is: juggle was placed under the 100-120 ℃ of condition 12-30 hour.
Further, the cultivation time among the step B is 25-30 days.
Further, among the step C, step B gained granular bacteria strain is put into culture bag, replenish a moisture content every day, and the addition of moisture content is no more than culture bag content gross weight, and 9-15 ℃ leaves standstill, and divides and wins mushroom 4-6 time.
Further, among the step C, in the moisture content that replenishes, contain vitamin and glucose.
Beneficial effect of the present invention is: this method is simple to operate, and the mushroom yield is high.More beneficial effects are please with reference to embodiment.
Embodiment
Lentinus alternating temperature fecundity mushroom.The mycelial growth temperature scope is wider, is 5 ℃～32 ℃, and thermophilic is 25 ℃～27 ℃, and fruit body has been sent out temperature between 5 ℃～22 ℃, is optimum with about 15 ℃.Alternating temperature can promote the fruit body differentiation.Temperature is too high, and mushroom growth is fast, but the thin handle of meat is long of poor quality, and it is slow to grow during low temperature, and cap is plump.Quality is closeer.
Mushroom mycelium growing period humidity is low during than fruiting; The composts or fertilisers of cultivating water content of suitable mycelial growth is about 60%～65%, and relative air humidity is about 70%, and relative air humidity will keep 85%～90% for suitable during the fruiting; Certain humidity is poor, helps mushroom growth and grows.
Mushroom also is the aerobe silk, though to carbonic acid gas not as sensitivity such as glossy ganoderma, if air is not smooth, the carbonic acid gas productive set is too much in the environment, will suppress the formation of mycelial growth and fruit body, even cause assorted bacterium evil life.So draughty place should be selected in the mushroom field, to guarantee that mushroom grows normally.
Mushroom is the heliophilic mushroom.Though mushroom mycelium also can be grown under dark condition; But fruit body then can not take place, and only under appropriate illumination, fruit body could successfully be grown, and the spore that sheds.But strong direct light all is disadvantageous to mycelial growth and fruiting.
The mushroom mycelium growth requires the environment of meta-acid.Mycelia all can grow between PH3～7, and is the most suitable up and down with pH4.5.During mushroom culture, the unsuitable basicity in place is excessive.When spraying water, mushroom water logging water will be noted water quality; Prevent damage by disease and insect, preferably without basic agent.
The reproductive condition of mushroom interacts, interrelates.To the fruit body forming process, temperature is that high earlier back is low from mycelial growth, and humidity is to do afterwards earlier to wet, and light is that dark earlier back is bright.These conditions both connected each other, and mutual restriction must be considered comprehensively again, in order to avoid attend to one thing and lose sight of another, just can get a desired effect.
Further set forth the present invention below in conjunction with embodiment.Should be understood that these embodiment only are used to explain the present invention, and unrestricted scope of the present invention.
One preliminary treatment
Choosing diameter is the broad-leaved branch of 8-12 millimeter, and the juggle that is cut into 2-3 centimetre is put into the aseptic culture bag after sterilization treatment; Mushroom has and decomposes lignin, cellulosic ability in the timber, can be glucose, amino acid etc. with its decomposition and inversion, the nutriment that directly absorbs and utilize as hyphal cell.Use frequency to be 40kHz ultrasonic Treatment 20 minutes before the sterilization, be more conducive to hyphal cell and effectively absorb and utilize lignin in the timber, cellulosic ability.
The preparation of two granular bacteria strains
Mother's kind of mushroom is received on the juggle of preliminary treatment and cooling, under 20-25 ℃ of condition, cultivated to mycelia and cover with juggle, get granular bacteria strain.
Mother's kind of mushroom is received in the culture that wood chip is a matrix, granular bacteria strain is in the follow-up cultivation of mushroom, and is more manageable; And during secondary inoculation, the lacking of the time ratio wood chip matrix that solid particle bacterial classification inoculation operation is spent.
Three enlarged culture
The gained granular bacteria strain is put into culture bag; The composts or fertilisers of cultivating that mushroom needs (78 parts of wood chips composed of the following components by weight, 20 parts in wheat bran (fine rice bran), 1 part in gypsum, 1 part of sugar, other adds 0.3 part in urea, the quality that contains of composts or fertilisers of cultivating is 55%～60%) is arranged in the culture bag; Left standstill 60 days at shady and cool place; Replenish a moisture content every day, and the addition of moisture content is no more than culture bag content gross weight, wins first mushroom.Everyday several at interval, win second batch of mushroom, pluck 6 batches altogether.In the moisture content that replenishes, contain the water soluble vitamin compound of 100mg/L and the glucose of 200g/L.3 jin of mushrooms of per 4 jin heavy composts or fertilisers of cultivating results.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although with reference to preferred embodiment the present invention is specified, those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention; And not breaking away from the aim and the scope of technical scheme of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (6)
1. the mushroom method of cultivating is characterized in that having by following steps and form:
The preliminary treatment of A material
Choosing diameter is the broad-leaved branch of 8-12 millimeter, and the juggle that is cut into 2-3 centimetre is put into the aseptic culture bag after sterilization treatment;
The preparation of B granular bacteria strain
Mother's kind of mushroom is received on the juggle of processing of step A and cooling, under 20-25 ℃ of condition, cultivated to mycelia and cover with juggle, get granular bacteria strain;
The C enlarged culture
Step B gained granular bacteria strain is put into culture bag, and left standstill 55-65 days at shady and cool place, wins mushroom.
2. the method that mushroom according to claim 1 is cultivated is characterized in that, the juggle in the steps A uses frequency to be 40-60kHz ultrasonic Treatment 15-30 minute before the sterilization.
3. the method that mushroom according to claim 1 is cultivated is characterized in that in the steps A, the method for sterilization is: juggle was placed under the 100-120 ℃ of condition 12-30 hour.
4. the method that mushroom according to claim 1 is cultivated is characterized in that the cultivation time among the step B is 25-30 days.
5. the method that mushroom according to claim 1 is cultivated is characterized in that, among the step C; Step B gained granular bacteria strain is put into culture bag, and replenish a moisture content every day, and the addition of moisture content is no more than culture bag content gross weight; 9-15 ℃ leaves standstill, and divides and wins mushroom 4-6 time.
6. the method that mushroom according to claim 5 is cultivated is characterized in that, among the step C, in the moisture content that replenishes, contains vitamin and glucose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210269693.5A CN102771311B (en) | 2012-07-31 | 2012-07-31 | Shiitake cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210269693.5A CN102771311B (en) | 2012-07-31 | 2012-07-31 | Shiitake cultivation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102771311A true CN102771311A (en) | 2012-11-14 |
| CN102771311B CN102771311B (en) | 2016-09-14 |
Family
ID=47116629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210269693.5A Active CN102771311B (en) | 2012-07-31 | 2012-07-31 | Shiitake cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102771311B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230519A (en) * | 2014-08-03 | 2014-12-24 | 青岛锐志农业技术服务有限公司 | Method for preparing culture medium for edible mushroom |
| CN105110855A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Lentinula edodes culture medium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113676A (en) * | 1994-06-22 | 1995-12-27 | 朱吉权 | Producing technology for lentinus edodes with surface pattern |
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
-
2012
- 2012-07-31 CN CN201210269693.5A patent/CN102771311B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113676A (en) * | 1994-06-22 | 1995-12-27 | 朱吉权 | Producing technology for lentinus edodes with surface pattern |
| CN102047814A (en) * | 2010-10-25 | 2011-05-11 | 青岛农业大学 | Micro ventilation lime wood antrodia camphorate cultivation method |
Non-Patent Citations (2)
| Title |
|---|
| 彭洪光: "利用树枝栽培香菇的研究", 《西南师范大学学报》 * |
| 王华卿: "香菇枝条菌种的制作", 《山西科技报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230519A (en) * | 2014-08-03 | 2014-12-24 | 青岛锐志农业技术服务有限公司 | Method for preparing culture medium for edible mushroom |
| CN105110855A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Lentinula edodes culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102771311B (en) | 2016-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103918475B (en) | The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom | |
| CN102630481B (en) | Cultivation method for oospore oudemansiella mucida | |
| CN105532257A (en) | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method | |
| CN105474995A (en) | Cultivation and domestication method of wild collybia albuminosa | |
| CN106613349A (en) | Tremella aurantialba substitute cultivation method | |
| CN106818207B (en) | A kind of bag cultivation growing straight method of needle mushroom | |
| CN103907478A (en) | Method and medium for cultivating oyster mushrooms in potted landscape manner | |
| KR100823541B1 (en) | Mushroom cultivation method | |
| CN107079711B (en) | Cultivation method of morchella | |
| CN102613004A (en) | Method using pleurotus nebrodensis waste to cultivate flammulina velutipes | |
| CN109769592B (en) | Lucid ganoderma cultivation method | |
| CN101496486B (en) | Method for cultivating mushroom | |
| CN102487727A (en) | Method for producing pleurotus eryngii quel strains | |
| CN108450230A (en) | A kind of cultural method of oyster mushroom | |
| CN111328627A (en) | Direct regeneration culture method for tremella aurantialba sporocarp | |
| CN104303833A (en) | Method for cultivating Taiwan pleurotus geesteranus in north of Anhui province | |
| CN103283481A (en) | Infecting method of pinus massoniana seedling by cantharellus cibarius | |
| CN104982178A (en) | Method for interplanting panus giganteus in sugarcane field | |
| CN117016287A (en) | Efficient fruiting method for tremella aurantialba grafting | |
| CN102771311B (en) | Shiitake cultivation method | |
| KR20100061386A (en) | Fungal bed cultivation method of hon-shimeji mushroom | |
| CN103396218A (en) | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost | |
| CN102771312A (en) | Shiitake inoculation method | |
| KR20110005618U (en) | The mushroom seed germiculture vinyl bag for mushroom culture | |
| CN106635826A (en) | Preparation method of edible fungi cultivation strain based on recycling of disposable bamboo chopsticks |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |