CN102757905A - Kitchen waste HBS-Hab(pichia pastoris) and application thereof - Google Patents
Kitchen waste HBS-Hab(pichia pastoris) and application thereof Download PDFInfo
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- CN102757905A CN102757905A CN2012101100864A CN201210110086A CN102757905A CN 102757905 A CN102757905 A CN 102757905A CN 2012101100864 A CN2012101100864 A CN 2012101100864A CN 201210110086 A CN201210110086 A CN 201210110086A CN 102757905 A CN102757905 A CN 102757905A
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Abstract
The invention relates to kitchen waste HBS-Hab(pichia pastoris), of which the preservation number is CGMCC No.5960. The HBS-Hab(pichia pastoris) has a high-temperature fermentation capability, can fast decompose lactic acid, amino acid, organism, protein and other matrixes at the temperature of 60 DEG C, has a higher breading capability than other yeasts, and can coexist with lactobacillus so as to be significant to promote the appreciation of the lactobacillus; furthermore, the HBS-Hab(pichia pastoris) is mainly in charge of the high-temperature fermentation of the kitchen waste to accelerate the decomposition rate and promote the appreciation of effective microorganisms. The HBS-Hab(pichia pastoris) belongs to non-toxic strains, and has an obvious aerobic treatment effect on the kitchen waste and a lower treatment cost, thereby having a great practical and population value.
Description
Technical field
The invention belongs to field of microbial biotechnology, particularly a kind of yeast and application thereof.
Background technology
The changing food waste staple comprises rice and flours food residues, vegetables, vegetable and animals oils, meat bone etc., on chemical constitution, starch, Mierocrystalline cellulose, protein, lipid and inorganic salt is arranged.Principal feature is that organic content is abundant, moisture content is high, perishable, and its proterties and smell all can make a very bad impression to environmental health, and grow pathogenic micro-organism easily, objectionable impurities such as mycotoxin.
Owing to the cooking culture and the custom of having a dinner party, produce the changing food waste of flood tide every day.The annual changing food waste that produces of Chinese city is not less than 6,000 ten thousand tons.Nutritious changing food waste is valuable renewable resources.But owing to do not draw attention as yet, method of disposal is improper, and it has become influences the potentially dangerous of food safety and ecological security source.Changing food waste has the dual nature of refuse and resource, can be described as typical " having misplaced local resource ".And unprocessed directly raising livestock and poultry can bring harm to HUMAN HEALTH through the accumulation of livestock and poultry vivotoxin, objectionable impurities again, thereby cause the cross infection between the people and animals.Contain toxic substances such as flavacin, benzene in " sewer oil " that also has present illegal retailer to sell, get back to people's dining table through underground approach, human consumption causes the generation of chronic disease even carcinogenic, and people healthy caused great harm.
On September 22nd, 2011, Standing Committee of Beijing Municipal People's Congress's review " Beijing's managing household garbage regulations (draft) ".Be to cut off sewer oil and flow into the channel of dining table, draft requires that certain scale is qualified should build rubbish on-the-spot disposal facility with regard to the meal.The water cut of changing food waste is high, is prone to corruption, and stink is big, and easy row becomes secondary pollution, the inconvenience of also bringing for its transportation.Therefore, how rapid Continuous is carried out the original position harmless treatment of changing food waste effectively, becomes the current social problem demanding prompt solution.In recent years, in changing food waste aerobic treatment process, because changing food waste has the corruption of being prone to, becomes sour, cause the environment of treatment media very easily to change, make the microbic activity resolving power descend, to produce malodorous smell and treatment effect undesirable thereupon.
Existing to changing food waste processing microbial inoculum, adopt conducts such as genus bacillus, pseudomonas to clear up bacterial strain mostly, because these bacterial classification resistance to acids are not strong; The ability that suppresses the harmful microorganism growth; Cause the treatment time short, handle filler and add the easy step-down of pH in the process, cause the microorganism growth environmental change at changing food waste; Resolving power descends, and treatment effect is undesirable.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of changing food waste to subdue the type yeast.
An also purpose of the present invention is to provide a kind of changing food waste to subdue the application of type yeast in decomposing changing food waste.
In order to realize the object of the invention, the present invention also provides a kind of changing food waste to subdue type yeast HBS-Hab (Pichia pastoris), and its preserving number is CGMCC No.5960.
Said preserving number is that the yeast of CGMCC No.5960 has the thermophilic fermentation ability; Can be under 60 ℃ temperature matrix such as decomposing lactic acid, amino acid, organism, protein fast, it is stronger to compare other yeast prolificacies, this bacterium can coexist with milk-acid bacteria; To promoting the milk-acid bacteria increment to have very important meaning; This bacterium mainly is responsible for the thermophilic fermentation of changing food waste, accelerates rate of decomposition, promotes the increment of effective microbe.Said bacterial classification is spherical bacterium, and bacterium colony is an oyster white on wort agar, tarnish, and there is thin breach at the edge.In wort, cultivate, the nutrient solution surface have white and the wrinkle coarse bacterium uncut jade, bacterial sediment is arranged at the end.
A kind of changing food waste of the present invention is subdued type milk-acid bacteria HBS-RS (Pediococcus acidilactici), and its preserving number is CGMCC No.5959.
Said preserving number is that the milk-acid bacteria of CGMCC No.5959 can decomposition glucose and protein; It is transformed into lactic acid more than 50%; Therefore breeding under sour environment suits; And have a very strong capacity antacid, and can, the pH value survive in being 3 environment, and compare other milk-acid bacterias and have more acid resistance.
Above-mentioned milk-acid bacteria is a spherical shape, and two planes alternately divide formation tetrad shape at the right angle, and general cell is given birth in pairs, and single survivor is rare, does not become catenation.Gram-positive is not moved, and produces acid, amphimicrobian.Bacterium colony is little on the MRS substratum, is white in color.Grower along agar puncture line is thread.Catalase is negative, does not produce cytopigment.
On the other hand; The present invention also provides a kind of changing food waste to subdue the type complex microbial inoculum, and it comprises following components in weight percentage: milk-acid bacteria (Pediococcus acidilactici) 0.5%-2%, yeast (Pichia pastoris) 0.5%-1.5%; Rice bran 40%-50%; Wheat bran 40-50%, beans cypress 1%-10%, protein powder 1%-10%; The effective content of said milk-acid bacteria is 1.5 * 10
6-3.0 * 10
6Cfu/g, said zymic effective content is 1.0 * 10
6-3.5 * 10
6Cfu/g.
Also on the one hand, the invention provides a kind of changing food waste and subdue the application of type yeast in decomposing changing food waste.
Beneficial effect of the present invention:
Yeast provided by the invention has the thermophilic fermentation ability; Can be under 60 ℃ temperature matrix such as decomposing lactic acid, amino acid, organism, protein fast, it is stronger to compare other yeast prolificacies, this bacterium can coexist with milk-acid bacteria; To promoting the milk-acid bacteria increment to have very important meaning; This bacterium mainly is responsible for the thermophilic fermentation of changing food waste, accelerates rate of decomposition, promotes the increment of effective microbe.Saccharomycodes of the present invention is in non-toxic strain, and is obvious to changing food waste aerobic treatment effect, reduces processing cost, so very big practical and popularizing value is arranged.
Description of drawings
Fig. 1 handles 60 days rubbish weight change figure for composite fungus agent of the present invention;
Fig. 2 handles 30 days rubbish weight change figure for yeast of the present invention.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.Under the situation that does not deviate from the present invention's spirit and essence, modification or replacement to the inventive method, step or condition are done all belong to protection scope of the present invention.
If do not specialize the conventional means that used technique means is well known to those skilled in the art among the embodiment.
Said milk-acid bacteria and yeast all separate acquisition from swill, the invention discloses a kind of milk-acid bacteria (Pediococcus acidilactici), and its preservation name is called HBS-RS, preserving number: CGMCC No.5959.The invention also discloses a kind of pichia pastoris (Pichia pastoris), its preservation name is called HBS-Hab, preserving number: CGMCC No.5960.Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date: on April 9th, 2012.
Embodiment 2 changing food wastes are subdued the type complex microbial inoculum
1) slant culture:, cultivated 2 days under 37 ℃ of conditions with being inoculated in respectively on the solid medium under yeast among the embodiment 1 and the milk-acid bacteria original strain aseptic condition;
2) first order seed is cultivated: be inoculated in liquid nutrient medium under the bacterial classification aseptic condition with the step 1) cultivation, under 42 ℃ of conditions, the 150r/min shaking table was cultivated 1 day, yeast and milk-acid bacteria suspension liquid light density OD when finishing to cultivate
600Value all reaches 4.0;
3) secondary seed is cultivated: be 10% inoculum size by the volume ratio of liquid nutrient medium; First order seed is inoculated into respectively in the fermentor tank of 1000L, the TV of nutrient solution is 500L in the fermentor tank, under 42 ℃ of conditions of yeast and milk-acid bacteria; Stirring velocity is 150r/min; Air flow is 1: 1, cultivates 1 day, makes secondary seed;
Wherein, Step 1), 2) yeast and the used substratum of milk-acid bacteria are proteolytic enzyme peptone (protease peptone) 1g in, beef extract (beef extract) 1g, glucose (glucose) 2g; Sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The prescription of the substratum that step 3) is used is by mass percentage: Semen Maydis powder 2.5%, soyflour 1%, peptone 1%, yeast powder 2.5%, K
2HPO
40.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.4.
The fermentation culture process comprises: in initial 12 hours, ventilation at interval remains on the aerobic conditions fermentation, air flow 1: 1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, stirred 42 ℃ of temperature 2 minutes at 2 hours mixing chamber intervals; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation is stirred at interval, 4 hours mixing chamber intervals, stirs 42 ℃ of temperature 4 minutes.
500 liters are expanded adding solid fermentation substratum 7500kg in numerous culture bacteria liquid; Said solid fermentation substratum is milk-acid bacteria Pediococcus acidilactici1.5%; Yeast Pichia pastoris 1%, rice bran (46%), wheat bran (45.5%), beans cypress (5%), protein powder (1%) stir, and use the film capping; Every at a distance from stirring in 24 hours, cultivated 72 hours.
Embodiment 3
Expand numerous bacterial classification according to liquid fermentation process in the instance 2.
Wherein, Step 1), 2) yeast and the used substratum of milk-acid bacteria are proteolytic enzyme peptone (protease peptone) 1.5g in, beef extract (beef extract) 0.5g, glucose (glucose) 1g; Sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The prescription of the substratum that step 3) is used is by mass percentage: Semen Maydis powder 1%, soyflour 1%, peptone 1%, yeast powder 1.5%, K
2HPO
40.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.4.
The fermentation culture process comprises: in initial 12 hours, ventilation at interval remains on the aerobic conditions fermentation, air flow 1: 1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, stirred 41 ℃ of temperature 2 minutes at 2 hours mixing chamber intervals; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation is stirred at interval, 4 hours mixing chamber intervals, stirs 41 ℃ of temperature 4 minutes.
Expand numerous culture bacteria liquid with 500 liters and join (7500kg-8000kg) in the solid fermentation substratum.Said solid fermentation substratum is milk-acid bacteria Pediococcus acidilactici1.5%, yeast Pichia pastoris1%, rice bran (40%), wheat bran (40%), beans cypress (7.5%), protein powder (10%); Stir; Use the film capping, every at a distance from stirring in 24 hours, cultivated 48 hours.
Embodiment 4
Expand numerous bacterial classification according to liquid fermentation process in the instance 2.
Wherein, Step 1), 2) yeast and the used substratum of milk-acid bacteria are proteolytic enzyme peptone (protease peptone) 1g in, beef extract (beef extract) 1g, glucose (glucose) 2g; Sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The prescription of the substratum that step 3) is used is by mass percentage: Semen Maydis powder 2.5%, soyflour 2.5%, peptone 2%, yeast powder 2.5%, K
2HPO
41.5%, MgSO
40.005%, tween 80 1%, sodium-acetate 0.5%, surplus are water, pH6.6.
The fermentation culture process comprises: in initial 12 hours, ventilation at interval remains on the aerobic conditions fermentation, air flow 1: 1, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, stirred 40 ℃ of temperature 2 minutes at 2 hours mixing chamber intervals; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation is stirred at interval, 4 hours mixing chamber intervals, stirs 40 ℃ of temperature 4 minutes.
Expand numerous culture bacteria liquid with 500 liters and join 7800kg in the solid fermentation substratum.Said solid fermentation substratum is milk-acid bacteria Pediococcus acidilactici1.5%; Yeast Pichia pastoris1%, rice bran (48%), wheat bran (47.5%), beans cypress (1%), protein powder (1%) stir, and use the film capping; Every at a distance from stirring in 24 hours, cultivated 66 hours.
Expand numerous bacterial classification according to liquid fermentation process in the instance 2.
Wherein, Step 1), 2) yeast and the used substratum of milk-acid bacteria are proteolytic enzyme peptone (protease peptone) 1g in, beef extract (beef extract) 1g, glucose (glucose) 2g; Sodium acetate (sodium acetate) 3g, and Trisodium Citrate (ammoni acitrate) 2g.
The prescription of the substratum that step 3) is used is by mass percentage: Semen Maydis powder 1.0%, soyflour 2%, peptone 2.5%, yeast powder 2%, K
2HPO
41.5%, MgSO
40.1%, tween 80 1.5%, sodium-acetate 1.5%, surplus are water, pH6.8.
The fermentation culture process comprises: in initial 12 hours, ventilation at interval remains on the aerobic conditions fermentation, air flow 1: 1.2, and regulation and control fermentation dissolved oxygen 10%, mixing speed 180r/min, stirred 42 ℃ of temperature 2 minutes at 2 hours mixing chamber intervals; Slightly soluble oxygen and anaerobism cultivation stage: after 12 hours, keep fermented liquid upper strata slightly soluble oxygen condition, static cultivation is stirred at interval, 4 hours mixing chamber intervals, stirs 42 ℃ of temperature 4 minutes.
Expand numerous culture bacteria liquid with 500 liters and join 8000kg in the solid fermentation substratum.Said solid fermentation substratum is milk-acid bacteria Pediococcus acidilactici1.5%; Yeast Pichia pastoris1%, rice bran (43%), wheat bran (43%), beans cypress (6%), protein powder (5.5%) stir, and use the film capping; Every at a distance from stirring in 24 hours, cultivated 72 hours.
Application examples
(1) this time be 30 days experimental period; Cultivate flow process according to the solid fungicide of instance 2-5 and be made into product; To 4 kinds of product number consecutivelies is JH1, JH2, JH3, JH4; Every number of viable of adding up each microbial inoculum at a distance from 5 days, following diagram data show that the JH1 microbial inoculums of instance 2 correspondences are best to the absorption protection effect of mikrobe, and number of viable remained on 6.5 * 10 in 30 days
6Cfu/g, survival rate reaches 85%, and the JH3 microbial inoculum effect of instance 3 correspondences is the poorest, and number of viable dropped to 4.2 * 10 in 30 days
5Cfu/g, decrease in survival rate to 6.5%.The ordering that draws four kinds of cultural methods thus is JH1＞JH2＞JH4＞JH3, and best training method is an instance 2.
4 kinds of composite fungus agents of table 1 are handled 30 days number of viable
(2) this time be 14 days experimental period, in the aerobic changing food waste handler of 1kg treatment capacity, adds the changing food waste of 540g at least, the highest adding of Dan Tian 1746g continuously every day.In the middle of added rubbish such as chicken bone, Fishbone, dish class, rice, meat.Its method is: said bacterial classification is through liquid fermenting propagation, and field planting processes solid fungicide JH1 in solid filler, and this microbial inoculum is added in the changing food waste handler at the addition with 1%.From experiment effect, carrier microbial inoculum quality does not increase, and the non-stimulated smell of decomposition course produces, and it is more than 96% that whole changing food waste is cleared up rate.Add the front and back contrast from changing food waste, the changing food waste base conditioning is complete after 4 hours, does not have obviously residual.Material decomposition such as bone are slower, and decompose at least by the above time of two weeks for needs.In this section treating processes, water ratio tends towards stability, and temperature is in 50 deg.c in the handler carrier, and microorganism growth is good, no anaerobically fermenting phenomenon.
Table 2 is handled the rubbish total amount monitoring result after 14 days
| Experimental period (my god) | Gross weight (g) before handling | Add rubbish total amount (g) | Handle back gross weight (g) |
| 14 | ?7000 | ?8572 | ?7337 |
(3) this time be 28 days experimental period, and experimental installation is the aerobic changing food waste handler of day output 50kg.Its method is: said bacterial classification is through liquid fermenting propagation, and field planting processes solid fungicide JH1 in solid filler, and this microbial inoculum is added in the changing food waste handler at the addition with 1%.It is 450kg that filler adds total amount; 28 days processing utensil rubbishes total amounts are 1390kg; Last surplus total amount is 380kg, and changing food waste is in complete treated state, because a part self filler is also by the microbial consumption utilization; And, cause the surplus total amount to be lower than and add the filler total amount with the release of heat energy form.
Table 3 is handled the rubbish total amount monitoring result after 28 days
| Time (my god) | Add quantity of refuse (kg) | Add filler (kg) | Unspent amount (kg) | |
| 1 | 20 | 400 | ? | ? |
| 2 | 20 | ? | ? | ? |
| 3 | 20 | ? | ? | ? |
[0059]
| 4 | 30 | 50 | ||
| 5～26 | 50 | |||
| 27 | 100 | |||
| 28 | 100 | |||
| Total amount | 1390 | 450 | 380 | 100% |
(4) be 60 days this experimental period, and experimental installation is the aerobic changing food waste handler of day output 1kg.Its method is: make microbial inoculum according to table 4 method, microbial inoculum is being added in the changing food waste handler with 1% addition respectively, the filler gross weight is 7kg.Monitor its weight change situation every day.Can be found out that by Fig. 1 rubbish accumulative total was added 15kg in 60 days, the composite fungus agent processing power of HBS-1 is the strongest, and the rubbish semi-invariant is minimum, and weight is 6.2kg after 60 days, compares changing food waste and adds total amount, and rubbish decomposes fully.Yet, only adding the microbial inoculum of milk-acid bacteria, treatment effect is poor slightly, and weight is 8.7kg after 60 days, and processing rate is 89%.It is the poorest only to add saccharomycetic treatment group effect, and weight is 10.5kg after 60 days, and processing rate is 77%.Thus, can draw, add composite fungus agent and be better than the treatment effect that only adds single bacterium.
The matching method of table 4 different strain addition
(5) be 30 days this experimental period, and experimental installation is the aerobic changing food waste handler of day output 1kg, and an other strain Angel Yeast is buied from the supermarket.Its method is: make microbial inoculum according to table 5 method, microbial inoculum is being added in the changing food waste handler with 1% addition respectively, the filler gross weight is 7kg.Monitor its weight change situation every day.Can be found out that by Fig. 2 rubbish accumulative total was added 10kg in 30 days, added the microbial inoculum of yeast (Pichia pastoris), treatment effect is obvious relatively, and gross weight is 9.8kg after 30 days, and processing rate is 72%.The treatment group effect that adds Angel Yeast is relatively poor relatively, and weight is 12.6kg after 30 days, and processing rate is 44%.Thus, can draw, yeast treatment effect of the present invention is obvious relatively, the Angel Yeast that is superior to buying on the market.
The matching method of table 5 different strain addition
The above % unit all is weight percentage.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (3)
1. a changing food waste is subdued type yeast HBS-ab (Pichia pastoris), and its deposit number is CGMCC No.5960.
2. the application of yeast in decomposing changing food waste according to claim 1.
3. the application of yeast in the composite fungus agent of preparation decomposition changing food waste according to claim 1.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272834A (en) * | 2019-05-23 | 2019-09-24 | 浙江工业大学 | The odorless type microbial bacterial agent and its preparation method and application of kitchen garbage processing |
| CN111715679A (en) * | 2020-07-21 | 2020-09-29 | 杭州洁洁环保科技有限公司 | Circulating dehydration biochemical processor and control method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174401A (en) * | 2011-01-30 | 2011-09-07 | 宁波大学 | Microorganism composite zymocyte agent as well as preparation method and application thereof |
| CN102391950A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Food waste deodorization composite bacteria and preparation method thereof |
-
2012
- 2012-04-13 CN CN2012101100864A patent/CN102757905B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174401A (en) * | 2011-01-30 | 2011-09-07 | 宁波大学 | Microorganism composite zymocyte agent as well as preparation method and application thereof |
| CN102391950A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Food waste deodorization composite bacteria and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| PEYTON A. EGGLESTON ET AL: "Ecology and elimination of cockroaches and allergens in the home", 《JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY》 * |
| 王星等: "国内外餐厨垃圾的生物处理及资源化技术进展", 《环境卫生工程》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110272834A (en) * | 2019-05-23 | 2019-09-24 | 浙江工业大学 | The odorless type microbial bacterial agent and its preparation method and application of kitchen garbage processing |
| CN111715679A (en) * | 2020-07-21 | 2020-09-29 | 杭州洁洁环保科技有限公司 | Circulating dehydration biochemical processor and control method thereof |
| CN111715679B (en) * | 2020-07-21 | 2023-12-19 | 杭州洁洁环保科技有限公司 | Circulation dehydration biochemical treatment machine and control method thereof |
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