CN102731204B - Preparation method of foliar fertilizer promoting alfalfa seedling growth in acidic soil - Google Patents
Preparation method of foliar fertilizer promoting alfalfa seedling growth in acidic soil Download PDFInfo
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- CN102731204B CN102731204B CN201210196963.4A CN201210196963A CN102731204B CN 102731204 B CN102731204 B CN 102731204B CN 201210196963 A CN201210196963 A CN 201210196963A CN 102731204 B CN102731204 B CN 102731204B
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- acid
- alfalfa
- nutrient solution
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Abstract
The invention discloses a preparation method of a foliar fertilizer promoting alfalfa seedling growth in acidic soil. The method comprises: first preparing a root growth solution, a mineral nutrient solution and an organic nutrient solution, then respectively taking 1-2ml of the root growth solution, 1-2ml of the mineral nutrient solution, and 3-5ml of the organic nutrient solution, mixing them and adding water to a constant volume to 1L, thus forming the foliar fertilizer. The foliar fertilizer prepared by the invention can effectively promote the growth of alfalfa seedling roots in strongly acidic soil, protect root activity and cell structures, relieve alfalfa seedling poisoning, and promote alfalfa growth. In a soil environment with pH of 4.5, the foliar fertilizer of the invention is utilized to treat alfalfa having an average plant height of 5cm. The foliar fertilizer is sprayed once every 10 days and 2 times in total. 1-2L/m<2> of the fertilizer is sprayed every time. After 30 days of treatment, the alfalfa root biomass is increased by 1.5-1.8 times compared with a control one, and the aboveground biomass is increased by 1.0-1.4 times compared with the control one.
Description
Technical field
What the present invention relates to is a kind of leaf leaven of agricultural technology field, specifically a kind of foliage fertilizer preparation method who improves acid soil Alfalfa.
Background technology
Red Soils in Southern China district soil generally presents strongly-acid (pH < 5.0), the g and D that suppresses Alfalfa Root System, tip of a root passivation, root hair is short and thick, dietetic alimentation function reduces, and then inhibition Growth of Alfalfa, cause that alfalfa plants is short, poor growth, output be very low, seriously restricted the Alfalfa Growing in Southern Red Soil soil district.
At present, the relevant research report that promotes alfalfa to grow at acid soil is fewer, there is no effective technical measures.Through the literature search of prior art being found to ，Guo man of virtue and ability army and yellow found the state (2006), in " Acta Prataculture " the 15th 1 phase of volume (84-89 page), reported that in acid soil, adding lime regulates soil pH value, has obviously promoted the growth of alfalfa; Wang Xinhai etc. (2005) 《 Yunnan Prov Agriculture University journal " reported to mend in the survey articles of the 20th 5 phases of volume (705-709 page) and executed the growth that boron fertilizer can improve roots of alfalfa in acid soil.But use lime improvement soil cost very high, the feasibility that big area is used is low; And use separately boron fertilizer to promoting the amount of increase in production of acid soil clover very limited, therefore, these two kinds of methods are not all widely used in actual production, not yet find a kind of technical measures that can effectively promote acid soil Growth of Alfalfa at present.
In retrieval, also find, Chinese Patent Application No. is: 200910135796.0, denomination of invention is: a kind of efficient green foliage fertilizer and method for making thereof, this invention is a kind of efficient green foliage fertilizer: " it forms (in ton) by following proportioning: sodium nitrophenolate 8-10kg, α-naphthaleneacidsodium 2-4kg, ammonium nitrate 70-90kg, Tripotassium Citrate 70-90kg, trace element (in metal) 9-12kg, choline dichloride 40-60kg, kaolin surplus.First sodium nitrophenolate, α-naphthaleneacidsodium and Tripotassium Citrate is even in stirrer for mixing, then add other raw material evenly to obtain product of the present invention in stirrer for mixing.It has onset rapidly, the distinguishing feature of increase yield significantly, safety nuisance free." but this invention cannot be for promoting the growth of acid soil alfalfa seedling.
Summary of the invention
The present invention is directed to the deficiency and the problem that in background technology, exist, a kind of preparation method who improves the foliage fertilizer of acid soil Alfalfa is provided.Foliage fertilizer prepared by the inventive method is in south China soil severe acidification (pH < 5.0), the khoai that alfalfa growing is subject to obviously suppressing is used, can effectively promote the g and D of alfalfa seedlings root, improve the absorptive function of root system to nutrition, promote the growth of acid soil alfalfa.
The present invention is achieved by the following technical solutions:
Foliage fertilizer of the present invention, by root growth liquid, mineral nutrition, organic nutrient solution for cultivating and clear water mix and form, in every liter of this foliage fertilizer, contain root growth liquid 1-2ml, mineral nutrition 1-2ml, organic nutrient solution for cultivating 3-5ml, clear water is surplus, wherein: described root growth liquid is comprised of indole-3-acetic acid (IAA); Described mineral nutrition is comprised of zinc (Zn), calcium (Ca), boron (B); Described organic nutrient solution for cultivating is comprised of succsinic acid, citric acid, isoflavones;
Above-mentioned foliage fertilizer is prepared by following steps:
1) preparation of root growth liquid:
(1) take and draw indole-3-acetic acid (IAA), be dissolved in ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter (L) with clear water, forms described root growth liquid.
2, the preparation of mineral nutrition:
(1) take respectively the Zn containing zinc (Zn)
3(PO4)
2; The Ca of calcic (Ca)
3(PO4)
2, boric acid;
(2) by the Zn in described (1)
3(PO4)
2, Ca
3(PO4)
2be dissolved in completely in clear water,
(3) boric acid is added to the Zn in described (2)
3(PO4)
2and Ca
3(PO4)
2in mixed solution, with clear water, be settled to 1 liter (L), the mineral nutrition described in mineral.
3, the preparation of organic nutrient solution for cultivating:
(1) get respectively succsinic acid, citric acid, isoflavones;
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating.
4, foliage fertilizer preparation:
Get respectively root growth liquid, mineral nutrition and organic nutrient solution for cultivating, by its mixing and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa.
Further, described step 1), is specially:
(1) sterling that takes 0.7g is drawn indole-3-acetic acid (IAA) (or non-sterling IAA of suitable content), is dissolved in a small amount of 95% ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter (L) with clear water, forms described root growth liquid, contains IAA:4mmol/L in every liter of this root growth liquid.
Further, described step 2), be specially:
(1) take respectively the ZnSO containing 65g zinc (Zn)
4169g; MgSO containing 96g magnesium (Mg)
4512g; MnCl containing 55g manganese (Mn)
2126g;
(2) by the ZnSO in described (1)
4, MgSO
4and MnCl
2be dissolved in completely in 1L clear water, form described mineral nutrition, in every liter of this mineral nutrition, contain respectively Zn:1mol/L, Mg:4mol/L and Mn:1mol/L.
Further, described step 3), is specially:
(1) get respectively the pure succsinic acid of 2.4g (or non-sterling succsinic acid of suitable content), the pure citric acid of 3.8g (or non-sterling citric acid of suitable content) and the pure isoflavones of 4.4g (or non-sterling isoflavones of suitable content);
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating, in every liter of this organic nutrient solution for cultivating, contain respectively succsinic acid: 20mmol/L, citric acid: 20mmol/L and isoflavones: 10 μ mol/L.
Further, described step 4), is specially:
Get respectively 1-2ml root growth liquid, 1-2ml mineral nutrition, 3-5ml organic nutrient solution for cultivating, mix and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa, wherein, in this foliage fertilizer of every 1L, contain IAA:4-8 μ mol/L, Zn:1-2mmol/L, Ca:4-8mmol/L, B:1-2mmol/L, succsinic acid: 60-100 μ mol/L, citric acid: 60-100 μ mol/L, isoflavones: 30-50 μ mol/L.
Adopt the foliage fertilizer of the raising acid soil Alfalfa that aforesaid method of the present invention obtains, there is the acid soil of promotion clover root growth, strengthen the acidproof effect of coercing of clover.Under the edatope of pH4.5, utilize this foliage fertilizer to process the alfalfa that average plant height is 5cm, every 10 days, spray 1 time, spray time-division between the lights time, spray altogether 2 times, spray 1-2L/m at every turn
2, to process 30 days, Alfalfa Root System biomass increases 1.5-1.8 doubly than contrast (only spraying clear water), and ground biomass increases 1.0-1.4 doubly than contrast.
Embodiment
Below embodiments of the invention are elaborated: following examples be take technical solution of the present invention and implemented as prerequisite, provided detailed embodiment and process, but protection scope of the present invention are not limited to following embodiment.
Embodiment mono-:
1, the preparation of root growth liquid:
(1) sterling that takes 0.7g is drawn indole-3-acetic acid (IAA), is dissolved in a small amount of 95% ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter (L) with clear water, forms described root growth liquid, contains IAA:4mmol/L in every liter of this root growth liquid.
2, the preparation of mineral nutrition:
(1) take respectively the ZnSO containing 65g zinc (Zn)
4169g; MgSO containing 96g magnesium (Mg)
4512g; MnCl containing 55g manganese (Mn)
2126g;
(2) by the ZnSO in described (1)
4, MgSO
4and MnCl
2be dissolved in completely in 1L clear water, form described mineral nutrition, in every liter of this mineral nutrition, contain respectively Zn:1mol/L, Mg:4mol/L and Mn:1mol/L.
3, the preparation of organic nutrient solution for cultivating:
(1) get respectively the pure succsinic acid of 2.4g (or non-sterling succsinic acid of suitable content), the pure citric acid of 3.8g (or non-sterling citric acid of suitable content) and the pure isoflavones of 4.4g (or non-sterling isoflavones of suitable content);
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating, in every liter of this organic nutrient solution for cultivating, contain respectively succsinic acid: 20mmol/L, citric acid: 20mmol/L and isoflavones: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil Alfalfa:
Get respectively 1ml root growth liquid, 1ml mineral nutrition, 3ml organic nutrient solution for cultivating, mix and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa, wherein, in every 1 liter of this foliage fertilizer, contain IAA:4 μ mol/L, Zn:1mmol/L, Ca:4mmol/L, B:1mmol/L, succsinic acid 60 μ mol/L, citric acid: 60 μ mol/L and isoflavones: 30 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this growth regulator are set respectively, process 30 days Growth of Alfalfa situations in Table 1.
Described control treatment adopts the alfalfa that the average plant height of fresh water spraying is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2.
This described foliage fertilizer is processed the foliage fertilizer of the raising acid soil Alfalfa that adopts the embodiment of the present invention two preparations, sprays the alfalfa that average plant height is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2.
The Growth of Alfalfa situation of table 1 embodiment mono-
Embodiment bis-:
1, the preparation of root growth liquid:
(1) sterling that takes 0.7g is drawn indole-3-acetic acid (IAA), is dissolved in a small amount of 95% ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter (L) with clear water, forms described root growth liquid, contains IAA:4mmol/L in every liter of this root growth liquid.
2, the preparation of mineral nutrition:
(1) take respectively the ZnSO4169g containing 65g zinc (Zn); MgSO containing 96g magnesium (Mg)
4512g; MnCl containing 55g manganese (Mn)
2126g;
(2) by the ZnSO in described (1)
4, MgSO
4and MnCl
2be dissolved in completely in 1L clear water, form described mineral nutrition, in every liter of this mineral nutrition, contain respectively Zn:1mol/L, Mg:4mol/L and Mn:1mol/L.
3, the preparation of organic nutrient solution for cultivating:
(1) get respectively the pure succsinic acid of 2.4g (or non-sterling succsinic acid of suitable content), the pure citric acid of 3.8g (or non-sterling citric acid of suitable content) and the pure isoflavones of 4.4g (or non-sterling isoflavones of suitable content);
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating, in every liter of this organic nutrient solution for cultivating, contain respectively succsinic acid: 20mmol/L, citric acid: 20mmol/L and isoflavones: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil Alfalfa:
Get respectively 1.5ml root growth liquid, 1.5ml mineral nutrition, 4ml organic nutrient solution for cultivating, mix and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa, wherein, in every 1 liter of this foliage fertilizer, contain IAA:6 μ mol/L, Zn:1.5mmol/L, Ca:6mmol/L, B:1.5mmol/L, succsinic acid 80 μ mol/L, citric acid: 80 μ mol/L and isoflavones: 40 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this foliage fertilizer are set respectively, process the Growth of Alfalfa situation of 30 days in Table 2.
Described control treatment adopts the alfalfa that the average plant height of fresh water spraying is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2.
This described foliage fertilizer is processed the foliage fertilizer of the raising acid soil Alfalfa that adopts the embodiment of the present invention one preparation, sprays the alfalfa that average plant height is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2.
The Growth of Alfalfa situation of table 2 embodiment bis-
Embodiment tri-:
1, the preparation of root growth liquid:
(1) sterling that takes 0.7g is drawn indole-3-acetic acid (IAA), is dissolved in a small amount of 95% ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter (L) with clear water, forms described root growth liquid, contains IAA:4mmol/L in every liter of this root growth liquid.
2, the preparation of mineral nutrition:
(1) take respectively the ZnSO containing 65g zinc (Zn)
4169g; MgSO containing 96g magnesium (Mg)
4512g; MnCl containing 55g manganese (Mn)
2126g;
(2) by the ZnSO in described (1)
4, MgSO
4and MnCl
2be dissolved in completely in 1L clear water, form described mineral nutrition, in every liter of this mineral nutrition, contain respectively Zn:1mol/L, Mg:4mol/L and Mn:1mol/L.
3, the preparation of organic nutrient solution for cultivating:
(1) get respectively the pure succsinic acid of 2.4g (or non-sterling succsinic acid of suitable content), the pure citric acid of 3.8g (or non-sterling citric acid of suitable content) and the pure isoflavones of 4.4g (or non-sterling isoflavones of suitable content);
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating, in every liter of this organic nutrient solution for cultivating, contain respectively succsinic acid: 20mmol/L, citric acid: 20mmol/L and isoflavones: 10 μ mol/L.
4, improve the foliage fertilizer preparation of acid soil Alfalfa:
Get respectively 2ml root growth liquid, 2ml mineral nutrition, 5ml organic nutrient solution for cultivating, mix and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa, wherein, in every 1 liter of this growth regulator, contain IAA:8 μ mol/L, Zn:2mmol/L, Ca:8mmol/L, B:2mmol/L, succsinic acid: 100 μ mol/L, citric acid: 100 μ mol/L and isoflavones: 50 μ mol/L.
Under the edatope of pH4.5, contrast and two processing of this foliage fertilizer are set respectively, process 30 days Growth of Alfalfa situations in Table 3.
Described control treatment adopts the alfalfa that the average plant height of fresh water spraying is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2;
This described foliage fertilizer is processed the foliage fertilizer of the raising acid soil Alfalfa that adopts the embodiment of the present invention three preparations, sprays the alfalfa that average plant height is 5cm, every 10 days, sprays 1 time, sprays altogether 2 times, sprays 1L/m at every turn
2.
The Growth of Alfalfa situation of table 3 embodiment tri-
Although content of the present invention has been done detailed introduction by above-described embodiment, will be appreciated that above-mentioned description should not be considered to limitation of the present invention.Those skilled in the art, read after foregoing, for multiple modification of the present invention with to substitute will be all apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (2)
1. a foliage fertilizer preparation method who promotes acid soil Alfalfa, it is characterized in that, described foliage fertilizer is by root growth liquid, mineral nutrition, organic nutrient solution for cultivating and clear water mix and form, and contain root growth liquid 1-2ml, mineral nutrition 1-2ml in every liter of this foliage fertilizer, organic nutrient solution for cultivating 3-5ml, clear water is surplus; Wherein: described root growth liquid is comprised of indole-3-acetic acid IAA; Described mineral nutrition is comprised of zinc Zn, calcium Ca, boron; Described organic nutrient solution for cultivating is comprised of succsinic acid, citric acid, isoflavones; In this foliage fertilizer of every 1L, contain IAA:4-8 μ mol/L, Zn:1-2mmol/L, Ca:4-8mmol/L, B:1-2mmol/L, succsinic acid: 60-100 μ mol/L, citric acid: 60-100 μ mol/L, isoflavones: 30-50 μ mol/L;
This foliage fertilizer is prepared by following steps:
1) preparation of root growth liquid:
(1) take and draw indole-3-acetic acid (IAA), be dissolved in ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter with clear water, forms described root growth liquid;
2) preparation of mineral nutrition:
(1) take respectively the Zn containing zinc Zn
3(PO
4)
2, calcic Ca Ca
3(PO
4)
2, boric acid;
(2) by the Zn in described (1)
3(PO
4)
2, Ca
3(PO
4)
2be dissolved in clear water completely;
(3) boric acid is added to the Zn in described (2)
3(PO
4)
2and Ca
3(PO
4)
2in mixed solution, with clear water, be settled to 1 liter, the mineral nutrition described in mineral;
3) preparation of organic nutrient solution for cultivating:
(1) get respectively succsinic acid, citric acid, isoflavones;
(2) succsinic acid in described (1), citric acid and isoflavones are mixed, with clear water, be settled to 1L, form described organic nutrient solution for cultivating;
4) foliage fertilizer preparation:
Get respectively root growth liquid, mineral nutrition and organic nutrient solution for cultivating, by its mixing and be settled to 1L with clear water, form a kind of foliage fertilizer that improves acid soil Alfalfa.
2. the foliage fertilizer preparation method of promotion acid soil Alfalfa according to claim 1, is characterized in that, described step 1), is specially:
(1) sterling that takes 0.7g is drawn indole-3-acetic acid IAA or the suitable non-sterling IAA of content, is dissolved in a small amount of 95% ethanol until dissolve completely;
(2) the IAA solution of being prepared by described (1), is settled to 1 liter with clear water, forms described root growth liquid, contains IAA:4mmol/L in every liter of this root growth liquid.
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| CN104150972B (en) * | 2014-05-23 | 2016-08-24 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of micro-fertilizer compound improving Tibet Three rivers area Alfalfa seed yield |
| CN104193549B (en) * | 2014-08-11 | 2016-07-06 | 上海交通大学 | A kind of bio-additive promoting alfalfa green manure to increase crop yield |
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| CN101941872A (en) * | 2010-08-19 | 2011-01-12 | 北京农学院 | Pesticidal foliage fertilizer mixed with plant growth factors and preparation method thereof |
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| CN101423443A (en) * | 2008-11-21 | 2009-05-06 | 深圳市朗钛生物科技有限公司 | Water soluble effervescence composition and method of making the same |
| CN101941872A (en) * | 2010-08-19 | 2011-01-12 | 北京农学院 | Pesticidal foliage fertilizer mixed with plant growth factors and preparation method thereof |
| CN102172129A (en) * | 2010-12-31 | 2011-09-07 | 苏州硒谷科技有限公司 | High-selenium alfalfa and cultivation method and application thereof |
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Effective date of registration: 20170105 Address after: 223001 science and Technology Industrial Park, Jiangsu, Huaian science and technology road, No. 18 Patentee after: North Jiangsu Institute of Shanghai Jiao Tong University Address before: 200240 Dongchuan Road, Shanghai, No. 800, No. Patentee before: Shanghai Jiaotong Univ. |