CN102696375B - Inoculation method for dipping or brushing roots by using polysaccharide-cistanche seed mixed solution - Google Patents
Inoculation method for dipping or brushing roots by using polysaccharide-cistanche seed mixed solution Download PDFInfo
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- CN102696375B CN102696375B CN 201210191003 CN201210191003A CN102696375B CN 102696375 B CN102696375 B CN 102696375B CN 201210191003 CN201210191003 CN 201210191003 CN 201210191003 A CN201210191003 A CN 201210191003A CN 102696375 B CN102696375 B CN 102696375B
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Abstract
The invention relates to an inoculation method for dipping or brushing roots by using a polysaccharide-cistanche seed mixed solution, belonging to the field of inoculation of cistanche onto haloxylon and tamarisk for desertification control. After the inoculation method is adopted, the cistanche inoculating success rate is improved. A polysaccharide thick solution is mixed with cistanche seeds; and the cistanche inoculating rate is improved by dipping the roots with the polysaccharide-cistanche seed mixed solution when the haloxylon or the tamarisk is planted and brushing the root system of the survived haloxylon or tamarisk with the polysaccharide-cistanche seed mixed solution.
Description
Technical field
The present invention relates to the method for control of desert sacsaoul and red building inoculating cistanche salsa, belong to cistanche salsa artificial inoculation technique field.
Background technology
Saline cistanche is the dry herb of orobanchaceae plant cistanche, calls desert cistanche, rues greatly, examines and do high want (Mongolian), and main parasitic is at the root of eremophytes sacsaoul and red building, and the succulent stem plumpness is so be called saline cistanche.It has kidney-replenishing, benefiting essence-blood, moisturizes effects such as leading to intestines, is to use the highest kidney-nourishing tcm drug of frequency.
Saline cistanche is perennial root holoparasite herbaceous plant, and desert cistanche itself can not carry out photosynthesis, relies on sucker to absorb nutrient and survive at host's root, colonizes in the Cistanche tubulosa that cries on the red building, colonizes in the title Desert Herba Cistanches on the sacsaoul.
Saline cistanche does not have autotrophy independence root system, and under field conditions (factors), cistanche deserticola seed is suave with the wind could be sprouted with the stimulation that needs to accept the host plant root exudates behind the rainwater infiltration desert.It is that sufficient nutrient and moisture will be arranged that cistanche deserticola seed grows one, the 2nd, and the newborn capillary tip of a root of host's sacsaoul extends to umbilical region in the saline cistanche just at the right time, discharges certain chemokinesis element, stimulates kind of an embryo splitting, and parasitic relation takes place, and develops into saline cistanche very soon.The parasitic success rate of saline cistanche is extremely low under field conditions (factors).For a long time and since excessively excavate with the natural propagation ability a little less than, cause wild saline cistanche resource to be in condition in imminent danger, be listed in Chinese Second Class Key Protected Plant.Simultaneously, be put into " international animals and plants trade pact in imminent danger " country and prohibite and excavate wild saline cistanche, encourage to solve resource problem by artificial planting.
Existing the most frequently used artificial infection method: the saline cistanche inoculation method mainly contains brokenly pico farad, the method for being completely cured, nutrition revulsion and inoculating paper inoculation method.
(1) broken pico farad is selected the above hair root of 0.4cm in the inoculation cave, scratching seed 10-20 grain that phloem will handle attaches at bast and scratches the place, the ABD root-growing agent or the 1000ppm acetylsalicylic acid solution-treated hair root that spray 50ppm concentration then reach sandy soil on every side, be backfilled to about near coal-mine 10cm after then 1 kilogram of rotten fertilizer being mixed with sandy soil, every hole irrigation water 10-15 kilogram, carry out mark after treating to infiltrate fully, earthing is treaded.
(2) method of being completely cured finds 1cm left and right sides hair root in the inoculation cave, undercut is disconnected, handle with the ABD root-growing agent aqueous solution of 100ppm concentration and to be completely cured and fibrous root, sandy soil on every side, the seed 40-60 grain of handling evenly is sprinkling upon the place of being completely cured, be backfilled to about the near coal-mine 10cm of distance after then the 1 kilogram of fertilizer that becomes thoroughly decomposed being mixed with sandy soil, every hole irrigation water 10-15 kilogram is carried out mark after Dai Wan full Seepage goes into, and earthing is treaded.
(3) nutrition revulsion is mixed and stirred the inoculation dish that clay is manufactured diameter 10cm, thick 2-3cm with nutrition inducing substances such as the 1000ppm acetylsalicylic acid aqueous solution, the seed 40-60 grain of handling evenly is sprinkling upon on the inoculation dish, on cover sandy soil and be placed on the intensive place of sacsaoul Root Distribution in the inoculation hole, ABD root-growing agent, ABT (3) number root-inducing powder aqueous solution sprinkling hair root with 50ppm concentration reach sandy soil on every side, be backfilled to about the near coal-mine 10cm of distance after then the 1 kilogram of fertilizer that becomes thoroughly decomposed being mixed with sandy soil, every hole irrigation water 10-15 kilogram, carry out mark after Dai Wan full Seepage goes into, earthing is treaded.
(4) inoculating paper inoculation method specific practice is: add water with clay (a kind of viscosity is very high, and water-permeable and air permeable is good, the mud that particle is little) and be stirred to certain viscosity, add an amount of seed and mix thoroughly.Evenly brush is on an about paper tape of 10 * 25 centimetres with mud with brush, and seed just evenly is coated with and has sticked on the paper tape like this.During inoculation paper tape is vertically planted in the other 50-70 centimetre of degree of depth that does not wait of host's root.Every hole irrigation water 10-15 kilogram is carried out mark after Dai Wan full Seepage goes into then, and earthing is treaded.Though because existing method can successfully be inoculated, success ratio of inoculation is not high.And time-consuming, cost is higher.Be difficult to form scale desert economy, influenced the plant trees enthusiasm of sand control of the masses.
The saline cistanche inoculation method that the present invention proposes, patent searching document and relevant document are not seen identical similar record.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing polysaccharide-cistanche deserticola seed mixed liquor artificial infection saline cistanche.The present invention has overcome the low and time-consuming shortcoming of success ratio of inoculation in the existing saline cistanche inoculation technique.A kind of mixed solution method of utilizing polysaccharide preparation and cistanche deserticola seed is provided.Utilize during to sacsaoul and red building plantation the mixed solution of polysaccharide preparation and cistanche deserticola seed to dip in method for root when being provided at sand control and planting trees.Provide sacsaoul and red building to surviving to utilize polysaccharide preparation and the mixed solution of cistanche deserticola seed to brush the inoculation method of root.
Concrete technical scheme of the present invention is as follows, uses polysaccharide cistanche deserticola seed mixed liquor to dip in the inoculation method of root or brush root, carries out according to following step:
(1) is mixed with sticky solution (1%) with polysaccharide (galactomannans), to and handle through seed selection and (the I and II cistanche deserticola seed of thousand kernel weight more than 0.13 gram be placed under the burning sun, be exposed to the sun more than ten hours, adopt then: A. soaked 60 minutes with the 500ppm naa; B. soaked 60 minutes with 500ppmABT; C. soaked 30 minutes with 100ppm ABD root-inducing powder.) cistanche deserticola seed sneak in the solution and stir;
(2) when sacsaoul or the plantation of red building seedling, dip in above-mentioned polysaccharide seed mixed liquor, cultivation method plantation routinely with root.
Perhaps step (2) is according to following processing: to the sacsaoul more than 3 years or the red building that has survived, cheat 2～3 in the plantation of digging dark 40～60cm, wide 30～40cm apart from selected sacsaoul or the big tree 50～80cm of red building plant place, find sacsaoul or red building root-room, open root system sandy soil on every side with hand plane, select the healthy and strong root of living as inoculation host root, dip in step (1) polysaccharide seed mixed liquor at rhizosphere with brush and smear, guarantee to be no less than 100 cistanche deserticola seeds and stick on the root system; Cover husky backfill after having smeared; Should not fill up during the earthing backfill, should leave the hole of 5～10cm, so that pouring and storing rainwater are carried out mark, so that observation and identification.
(3) beneficial effect
The inventive method has been compared following advantage with current methods:
1, the nothing of utilizing the moisture capacity of polysaccharide obviously to improve sacsaoul and the red building survival rate of irrigating plantation reaches more than 85%.Tradition plantation survival rate only about 20%.
2, sand control does not have to irrigate and utilizes polysaccharide-cistanche deserticola seed mixed liquor to dip in root plantation, inoculating cistanche salsa in afforestation when planting sacsaoul or red building.
3, utilize the nutritive effect promotion sacsaoul of polysaccharide and the well developed root system of red building, the success ratio of inoculation that obviously improves cistanche deserticola seed reaches more than 70%.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1 sacsaoul dips in root polysaccharide-cistanche deserticola seed aaerosol solution does not have the plantation of irrigation
The sticky solution of preparation polysaccharide (galactomannans), (1%) are placed after 20 minutes the cistanche deserticola seed stirring are spilled in the solution.Seed density is done a lab scale in earlier stage, and the seed suspension density can guarantee can enclose 30 cistanche deserticola seeds after every strain seedling dips in root in the solution.Dig dark 30 centimetres of plantation hole, sacsaoul seedling root is dipped in polysaccharide-cistanche deserticola seed suspension, guarantee to have on the root cistanche deserticola seed to adhere to.And guaranteeing to adhere to seed number is no less than 30 and plants into earthing then.Do not have the plantation of irrigation sacsaoul survival rate and reach more than 85%, inoculation survival rate of saline cistanche is more than 70%.
Embodiment 2 red buildings are dipped in root polysaccharide-cistanche deserticola seed aaerosol solution plantation
The preparation sticky solution of polysaccharide (galactomannans) (1%) is placed after 20 minutes the cistanche deserticola seed stirring is spilled in the solution.Seed density is done a lab scale in earlier stage, and the seed suspension density can guarantee can enclose 30 cistanche deserticola seeds after every strain seedling dips in root in the solution.Dig dark 30 centimetres of plantation hole, red building seedling root is dipped in polysaccharide-cistanche deserticola seed suspension, guarantee to have on the root Herba Cistanches seed to adhere to.And guaranteeing to adhere to seed number is no less than 30 and plants into earthing then.Do not have the plantation of irrigation red building survival rate and reach more than 85%, inoculation survival rate of saline cistanche is more than 70%.
Embodiment 3 survives the sacsaoul inoculating cistanche salsa
The preparation sticky solution of polysaccharide (galactomannans) (1%) is placed after 20 minutes the cistanche deserticola seed stirring is spilled in the solution.(seed density is done a lab scale in earlier stage, and the seed suspension density can be enclosed 300 cistanche deserticola seeds after guaranteeing to dip in brush in the solution.)。
Choose 3 years good sacsaouls of living upgrowth situation, press close to root and dig pit dark 50 centimetres, carefully clear up root system sand on every side with hand, dip in polysaccharide-cistanche deserticola seed aaerosol solution with brush and brush at root, guarantee that cistanche deserticola seed can adhere on the root system.Inoculation survival rate of saline cistanche is more than 70%.
Embodiment 4 survives the red building inoculating cistanche salsa
The preparation sticky solution of polysaccharide (galactomannans) (1%) is placed after 20 minutes the cistanche deserticola seed stirring is spilled in the solution.(seed density is done a lab scale in earlier stage, and the seed suspension density can be enclosed 300 cistanche deserticola seeds after guaranteeing to dip in brush in the solution.)。
Choose 3 years good red buildings of living upgrowth situation, press close to root and dig pit dark 50 centimetres, carefully clear up root system sand on every side with hand, dip in polysaccharide-cistanche deserticola seed aaerosol solution with brush and brush at root, guarantee that cistanche deserticola seed can adhere on the root system.Inoculation survival rate of saline cistanche is more than 70%.
Claims (3)
1. use polysaccharide cistanche deserticola seed mixed liquor to dip in the inoculation method of root or brush root, it is characterized in that carrying out according to following step:
(1) being mixed with mass concentration with polysaccharide is 1% sticky solution, will sneak in the solution and stirs through seed selection and the cistanche deserticola seed of handling;
(2) when sacsaoul or the plantation of red building seedling, dip in above-mentioned polysaccharide seed mixed liquor, cultivation method plantation routinely with root;
Step (2) is according to following processing: to the sacsaoul more than 3 years or the red building that has survived, cheat 2～3 in the plantation of digging dark 40～60cm, wide 30～40cm apart from selected sacsaoul or the big tree 50～80cm of red building plant place, find sacsaoul or red building root-room, open root system sandy soil on every side with hand plane, select the healthy and strong root of living as inoculation host root, dip in step (1) polysaccharide seed mixed liquor at rhizosphere with brush and smear, guarantee to be no less than 100 cistanche deserticola seeds and stick on the root system; Cover husky backfill after having smeared; Should not fill up during the earthing backfill, should leave the hole of 5～10cm, so that pouring and storing rainwater are carried out mark, so that observation and identification.
2. use polysaccharide cistanche deserticola seed mixed liquor according to claim 1 dips in the inoculation method of root or brush root, it is characterized in that the polysaccharide in the step (1) wherein is galactomannans.
3. use polysaccharide cistanche deserticola seed mixed liquor according to claim 1 dips in the inoculation method of root or brush root, the cistanche deserticola seed that it is characterized in that choosing seeds and handled refers to the I and II cistanche deserticola seed of thousand kernel weight more than 0.13 gram placed under the burning sun, is exposed to the sun more than ten hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210191003 CN102696375B (en) | 2012-06-12 | 2012-06-12 | Inoculation method for dipping or brushing roots by using polysaccharide-cistanche seed mixed solution |
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| CN 201210191003 CN102696375B (en) | 2012-06-12 | 2012-06-12 | Inoculation method for dipping or brushing roots by using polysaccharide-cistanche seed mixed solution |
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| CN102696375B true CN102696375B (en) | 2013-07-03 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472186B (en) * | 2014-12-10 | 2017-06-13 | 新疆林科院造林治沙研究所 | Artificial growth saline cistanche liquid inoculation method |
| CN108094064A (en) * | 2017-12-22 | 2018-06-01 | 西北师范大学 | In the method for moving dunes plantation sacsaoul inoculating cistanche salsa |
| CN112042480B (en) * | 2020-08-19 | 2022-12-02 | 云南农业大学 | Method for artificially planting Balanophora japonica |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044748A (en) * | 1990-02-06 | 1990-08-22 | 辽宁省商业科学技术研究所 | With the shitosan is the seed treatment agent manufacturing using method of raw material |
| CN101027967A (en) * | 2007-04-19 | 2007-09-05 | 和田帝辰沙生药物开发有限公司 | Inoculation of cistanche deserticola on tamarix ramosissima |
| CN102308712A (en) * | 2011-03-03 | 2012-01-11 | 中国林业科学研究院 | Cistanche compound inoculation method |
-
2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044748A (en) * | 1990-02-06 | 1990-08-22 | 辽宁省商业科学技术研究所 | With the shitosan is the seed treatment agent manufacturing using method of raw material |
| CN101027967A (en) * | 2007-04-19 | 2007-09-05 | 和田帝辰沙生药物开发有限公司 | Inoculation of cistanche deserticola on tamarix ramosissima |
| CN102308712A (en) * | 2011-03-03 | 2012-01-11 | 中国林业科学研究院 | Cistanche compound inoculation method |
Non-Patent Citations (4)
| Title |
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| 《"一可佳"增效剂在哈密瓜上的施用效果》;穆龙;《新疆农垦科技》;20081125(第6期);全文 * |
| 孙永强,田永祯,盛晋华等.《干旱荒漠区肉苁蓉人工接种技术研究》.《干旱区资源与环境》.2008,第22卷(第9期),全文. * |
| 田海舟，赵忠久，周新和，王昌霖.《管花肉苁蓉速效繁育科技示范研究》.《新疆林业》.2004,(第5期),全文. * |
| 穆龙.《"一可佳"增效剂在哈密瓜上的施用效果》.《新疆农垦科技》.2008,(第6期),全文. |
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