CN102676382A - Cell gas-liquid interface contact type cigarette whole smoke exposure device - Google Patents
Cell gas-liquid interface contact type cigarette whole smoke exposure device Download PDFInfo
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- CN102676382A CN102676382A CN201210171016XA CN201210171016A CN102676382A CN 102676382 A CN102676382 A CN 102676382A CN 201210171016X A CN201210171016X A CN 201210171016XA CN 201210171016 A CN201210171016 A CN 201210171016A CN 102676382 A CN102676382 A CN 102676382A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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Abstract
The invention relates to a cell gas-liquid interface contact type cigarette whole smoke exposure device, which belongs to the technical field of gas contamination culturing. The device mainly comprises a smoke diluting and transporting system and a cell culturing system, wherein the smoke diluting and transporting system consists of a smoke inlet (1), an air inlet (2) and a mixed smoke and air outlet (4) which are arranged on one side of a bottle body, a smoke and air mixing cavity (3) arranged in the bottle, and a mixed smoke and air delivery pipe mouth (5) arranged under the smoke and air mixing cavity (3); and the cell culturing system consists of a Transwell capsule bracket (6) connected with the mixed smoke and air delivery pipe mouth (5), a Transwell capsule (7) which has the diameter of 24mm and is connected with the Transwell capsule bracket (6), and a cell culture medium containing bottle (8) arranged under the Transwell capsule (7). The cell gas-liquid interface contact type cigarette whole smoke exposure device disclosed by the invention has the advantages of simple structure, convenience in operation and low cost, the device can be connected with a linear type smoking machine, and the cell contamination of gas-liquid interface contact type cigarette whole smoke exposure is realized.
Description
Technical field:
The present invention relates to the full fume exposure device of a kind of cell liquid-gas interface contact cigarette, belong to gas exposure culture technique field.
Background technology:
What be used for the cigarette smoke safety evaluation at present mainly is that three external toxicology detect (Ames test (Salmonella reversion test), vitro cell micronucleus test and toluylene red cell toxicity test); In these detected, the capture of flue gas sample and preparation had material impact to detected result.Classical sample captures and the method for preparation is to adopt smoking machine smoking cigarette, cambridge filter to capture the flue gas TPM; Use the flue gas TPM on DMSO 99.8MIN. (DMSO) the extraction cambridge filter again, detect at last to as if the cambridge filter of DMSO 99.8MIN. extraction on the flue gas TPM.What but the sample that this method captures was paid close attention to is the flue gas TPM, and the gaseous component of flue gas, the interaction between granule phase substance, gas phase thing and volatile matter is not taken into account.Therefore, full fume exposure method has appearred.This exposure chamber makes cigarette smoke be in " original " state (native) relatively, the relative situation of human body cell contact flue gas during anthropomorphic dummy's smoking, thereby more can truly reflect bacterium or the situation of cellular exposure in full smoke components.
Commercial full fume exposure device has the Cultex [1] of Japan and the Vitrocell [2] of Germany now; These two kinds of equipment are basic identical; Form by smoking machine, flue gas dilution distribution system, substratum supply system, substratum temperature maintenance system and cell/five parts of bacterial exposure device; This type device has adopted the transwell cell to come culturing cell, and cell is on the liquid-vapo(u)r interface and exposes, and cigarette smoke directly contacts with cell.This device can be realized full flue gas analysis of cigarette and environmental gas analysis, but this type of exposing device costs an arm and a leg complicated operation.
British American Tobacco has designed and produced full fume exposure device [3] voluntarily; Their exposing device has comprised flue gas recirculation system and cell/microbial culture system; The transwell cell has been adopted in the cultivation of pair cell, and cell is on the liquid-vapo(u)r interface and exposes, and cigarette smoke directly contacts with cell.This exposing device can reach full fume exposure experimental requirements, but this full fume exposure device only limits to the patent equipment of British American Tobacco, and not commercialization does not see that at present other research institutions use this equipment to carry out full fume exposure experimental study.
Imperial Tobacco Group has designed and has made the device [4] that directly 96 porocyte culture plates is carried out full fume exposure in addition, and cell attachment is grown in the culture hole bottom of 96 porocyte culture plates, and flue gas leads in each culture hole through the mode of similar flue gas injection.Flue gas is realized full fume exposure through nutrient solution and cells contacting.U.S. Battelle company has made full fume exposure device [4] with the Tissue Culture Flask repacking; Cell attachment is incubated in the Tissue Culture Flask; Flue gas feeds in the culturing bottle; Thereby the certain angle of vibration makes to flow about the nutrient solution in the culturing bottle and makes cell directly contact exposure with flue gas about vibrator, reaches the purpose that full flue gas directly exposes.In this exposure chamber, flue gas is not direct fully and cells contacting, and it is residual to have the part nutrient solution, thereby flue gas has part in nutrient solution, and this process for exposing is between the exposure chamber of the device of Cultex and Imperial Tobacco Group's exploitation.Domestic full fume exposure device " is used for the device ZL 200720092154.3 that the external toxicology main flume of cigarette exposes ", and improved " the full fume exposure contamination device of cigarette CN 201560188 U " and U.S. Battelle company are similar with carrying out on this basis.What " a kind of device ZL201120063251.6 that is used for the full fume exposure of cigarette " realized also is the gas-to-liquid contact of cigarette smoke and cell/bacterial suspension, the not direct fully and cells contacting of flue gas.
As stated; Present full fume exposure device can be realized that cell is on the liquid-vapo(u)r interface and expose; The direct exposing cell of flue gas has the Cultex of Japan, the Vitrocell of Germany and the full fume exposure device that British American Tobacco designs voluntarily and makes; But commercial have only the Cultex of Japan and the Vitrocell of Germany, but their apparatus structure complicated operation costs an arm and a leg; And British American Tobacco designs voluntarily and the full fume exposure device made is its oneself a patent equipment, and not commercialization does not see that at present other research institutions use this equipment to carry out full fume exposure experimental study yet.
Through the document retrieval, do not see the public reported identical with the present invention.
Reference:
[1]Cultex Laboratories.?http://www.cultex.com/.
[2]?Vitrocell?Systems?GmbH.?http://www.vitrocell.com/.
[3]Gaca?M,?Phillips?J,?Thorne?d,?et?al.?A?new?system?for?measuring?the?toxicity?of?mainstream?cigarette?smoke?[C].?2005?Coresta?Congress,?2005.
[4]?Coresta?in?vitro?toxicology?task?force.?In?vitro?exposure?of?cells?to?smoke?at?the?air?liquid?interface?[EB/OL].?http:///www.CORESTA.org.
Summary of the invention:
It is a kind of simple in structure that the object of the invention is to provide; The easy to operate full fume exposure device of cell liquid-gas interface contact cigarette; This device exposes cell on liquid-vapo(u)r interface, the direct exposing cell of flue gas truly reflects the situation after cell directly contacts full smoke components.
The full fume exposure device of cell liquid-gas interface contact cigarette of the present invention mainly comprises two portions: flue gas dilution delivery system and cell culture system; The flue gas dilution delivery system by place gas approach (1) on bottle one side, air (2) and mix back flue gas air-out (4), place a flue gas air mixed chamber (3) in the bottle, place the mixing of below, flue gas air mixed chamber (3) after flue gas air delivery pipe mouth (5) constitute; Cell culture system by with the Transwell capsule (7) of the diameter 24mm that mixes Transwell capsule support (6) that back flue gas air delivery pipe mouth (5) is connected, is connected with Transwell capsule support (6), place the cell culture medium below the Transwell capsule (7) to hold bottle (a 8) formation.Device forms with the Glass tubing blowing with current experiments by traditional technology, and the Transwell capsule of diameter 24mm is available from U.S. CORNING company.
The present invention has simple in structure, and is easy to operate, with low cost, can link to each other with the linear pattern smoking machine, realizes the advantage of the cell contamination of the full fume exposure of liquid-vapo(u)r interface contact cigarette.
Description of drawings:
Accompanying drawing is a structural representation of the present invention.
Embodiment:
The full fume exposure device of cell liquid-gas interface contact cigarette of the present invention mainly comprises two portions: flue gas dilution delivery system and cell culture system; The flue gas dilution delivery system by place gas approach 1 on bottle one side, air 2 and mix back flue gas air-out 4, place a flue gas air mixed chamber 3 in the bottle, place the mixing of 3 belows, flue gas air mixed chamber after flue gas air delivery pipe mouth 5 constitute; Cell culture system by with the Transwell capsule 7 of the diameter 24mm that mixes Transwell capsule support 6 that back flue gas air delivery pipe mouth 5 is connected, is connected with Transwell capsule support 6, place the cell culture medium of Transwell capsule 7 belows to hold bottles 8 to constitute.Device forms with the Glass tubing blowing with current experiments by traditional technology, and the Transwell capsule of diameter 24mm is available from U.S. CORNING company, and other parts that are attached thereto are that market is bought.
Full fume exposure device of this liquid-vapo(u)r interface contact cigarette and orthoscopic smoking machine be connected use, specific as follows:
Flue gas dilution delivery system and the clean oven dry of cell culture system back is subsequent use in the high-pressure sterilizing pot sterilization 1.;
2. place cell culture medium to hold bottle 8 cell culture medium of preheating, afterwards with whole exposing device as in 37 ℃ of water-baths;
3. the diameter that will cultivate cell when cell is contaminated is that the Transwell capsule 7 of 24mm keeps flat on the Transwell capsule support, guarantees that Transwell capsule bottom just contacts with cell culture medium;
4. straight line smoking machine flue gas incoming road sebific duct closely is connected on the smoke inlet 1;
5. filter one end closely is connected to exhanst gas outlet 4 places, again the filter the other end closely is connected with the smoking machine loop;
6. mixing air is connected to mixing air inlet 2 through sebific duct, flow velocity is by the minimum target flow velocity that transfers to;
7. set the automatic smoking machine program, the collection of beginning flue gas;
8. experiment finishes, and with the backward detaching device of erection sequence, deposits to carry out subsequent disposal reaction system is appropriate.
Practical application shows that this device reaches design requirements fully.
Claims (1)
1. full fume exposure device of cell liquid-gas interface contact cigarette; Mainly comprise flue gas dilution delivery system and cell culture system two portions, it is characterized in that: the flue gas dilution delivery system by place gas approach (1) on bottle one side, air (2) and mix back flue gas air-out (4), place a flue gas air mixed chamber (3) in the bottle, place the mixing of below, flue gas air mixed chamber (3) after flue gas air delivery pipe mouth (5) constitute; Cell culture system by with the Transwell capsule (7) of the diameter 24mm that mixes Transwell capsule support (6) that back flue gas air delivery pipe mouth (5) is connected, is connected with Transwell capsule support (6), place the cell culture medium below the Transwell capsule (7) to hold bottle (a 8) formation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210171016XA CN102676382A (en) | 2012-05-29 | 2012-05-29 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210171016XA CN102676382A (en) | 2012-05-29 | 2012-05-29 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
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| Publication Number | Publication Date |
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| CN102676382A true CN102676382A (en) | 2012-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210171016XA Pending CN102676382A (en) | 2012-05-29 | 2012-05-29 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
| CN106399076A (en) * | 2016-09-14 | 2017-02-15 | 南京大学 | Micro-fluidic gel gas-liquid interface flue gas exposing device |
| CN112525790A (en) * | 2020-10-26 | 2021-03-19 | 河南中烟工业有限责任公司 | Cigarette filter ventilation hole detecting system |
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| CN101580869A (en) * | 2009-07-01 | 2009-11-18 | 中国烟草总公司郑州烟草研究院 | Cigarette mainstream smoke genotoxicity measurement method |
| CN102140489A (en) * | 2011-01-24 | 2011-08-03 | 中国烟草总公司郑州烟草研究院 | Method for testing cytotoxicity in full smoke contamination of cigarette |
| CN102166374A (en) * | 2010-11-03 | 2011-08-31 | 山东省眼科研究所 | Method for preparing amniotic compound corneal limbus stem cell membrane |
| CN202054824U (en) * | 2011-03-11 | 2011-11-30 | 云南烟草科学研究院 | Device for whole cigarette smoke exposure |
| CN202671541U (en) * | 2012-05-29 | 2013-01-16 | 云南烟草科学研究院 | Cellular gas and liquid interface contact-type cigarette total-smoke exposure device |
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2012
- 2012-05-29 CN CN201210171016XA patent/CN102676382A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101580869A (en) * | 2009-07-01 | 2009-11-18 | 中国烟草总公司郑州烟草研究院 | Cigarette mainstream smoke genotoxicity measurement method |
| CN102166374A (en) * | 2010-11-03 | 2011-08-31 | 山东省眼科研究所 | Method for preparing amniotic compound corneal limbus stem cell membrane |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
| CN106399076A (en) * | 2016-09-14 | 2017-02-15 | 南京大学 | Micro-fluidic gel gas-liquid interface flue gas exposing device |
| CN112525790A (en) * | 2020-10-26 | 2021-03-19 | 河南中烟工业有限责任公司 | Cigarette filter ventilation hole detecting system |
| CN112525790B (en) * | 2020-10-26 | 2023-12-15 | 河南中烟工业有限责任公司 | Cigarette filter tip ventilation hole detecting system |
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Application publication date: 20120919 |