CN202054824U - Device for whole cigarette smoke exposure - Google Patents
Device for whole cigarette smoke exposure Download PDFInfo
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- CN202054824U CN202054824U CN2011200632516U CN201120063251U CN202054824U CN 202054824 U CN202054824 U CN 202054824U CN 2011200632516 U CN2011200632516 U CN 2011200632516U CN 201120063251 U CN201120063251 U CN 201120063251U CN 202054824 U CN202054824 U CN 202054824U
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- smoke
- cigarette
- flue gas
- exposure
- cigarette smoke
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Abstract
The utility model relates to a device for whole cigarette smoke exposure, and belongs to the technical field of exposed gas cultivation. The device comprises a cigarette smoke exposure bottle (6) and a smoke leading-in pipe stopper (7), wherein a smoke outlet (3) is arranged on the cigarette smoke exposure bottle (6); the lower half part of the smoke leading-in pipe stopper (7) is arranged in the cigarette smoke exposure bottle (6); and the smoke leading-in pipe stopper (7) is composed of a smoke inlet (1), a mixed air inlet (2), a smoke and air mixing cavity (4) and a diluted smoke conveying passageway (5). Compared with the prior art, the device for whole cigarette smoke exposure has the advantages that the structure is simple, the operation is convenient, the experimental difficulty is reduced, the cost is low, and the device can be connected to a linear smoking machine conveniently to obtain bacteria or cells.
Description
Technical field:
The utility model relates to a kind of device that is used for the full fume exposure of cigarette, belongs to gas exposure culture technique field.
Background technology:
The after chemical reaction in the very short time that flue gas produces of the aerosol that cigarette smoke is made up of several thousand kinds of chemical substances, these mixtures can produce countless qualitative changes and quantitative change [1], and wherein many components all have certain toxic action for cell or human body.External toxicologic study method is evaluated the potential impact [2] of experimental subjects to human body by measuring toxicant potential hazard or the damage of pair cell or DNA in a short time.Determination object comprises additive, tobacco extract, smoke condensate, flue gas gas phase thing and full flue gas.At present the external toxicologic study of cigarette smoke mainly concentrates on the genetoxic and the cytotoxicity testing research aspect of cigarette smoke, and these testing method are subjected to vast tobacco toxicology expert's consistent the approval, effectively the toxicity of evaluating cigarette product [3].
In the genetoxic and Cytotoxic test of the evaluating cigarette main flume of classics, the capture of flue gas sample mainly is on automatic smoking machine, capture the flue gas total particulate matter with cambridge filter, then the flue gas total particulate matter on the cambridge filter is eluted with DMSO, by cultivating, test the genetoxic and the cytotoxicity of flue gas with bacterium and cell.The advantage of this classical way is that the flue gas sample can prolonged preservation, shortcoming is the situation that the gained result can not represent authentic sample, because granule phase substance in test, the interaction of gas phase thing and volatile matter be not considered [4], after also having the long-time placement of flue gas, chemical ingredients can change.Therefore, just produced full fume exposure method.In full fume exposure method, the whole flue gases of cigarette directly are passed into the exposure container that cultivation has Salmonella typhimurium or test cell, its gas-liquid is dissolved each other or is allowed flue gas flow through the purpose that reaches full fume exposure with certain flow rate from media surface.This exposure chamber can in time reflect whole smoke components, and foreign tobacco company is developing full fume exposure device voluntarily, studies cytotoxicity and genetoxic [5] with evaluation of flue gas.Commercial Japanese Cultex[6 is arranged at present] and German Vitrocell[7] two brands, but complete assembly costs an arm and a leg, complicated operation.
The important research document of external full fume exposure device is as follows:
[1]Aufderheide，M.，An?efficient?approach?to?study?the?toxicological?effects?of?complex?mixtures.Experimental?and?Toxicologic?Pathology，2008.60(2008)：p.163-180.
[2]Aufderheide，M.and?H.Gressmann，A?modified?Ames?assay?reveals?the?mutagenicity?of?native?cigarette?mainstream?smoke?and?its?gas?vapour?phase.Experimental?and?Toxicologic?Pathology，2007.58(6)：p.383-392
[3]Aufderheide，M.，J.W.Knebel，and?D.Ritter，Novel?approaches?for?studying?pulmonary?toxicity?in?vitro.Toxicology?Letters，2003.140-141：p.205-211
[4]In?vitro?exposure?of?cells?to?Smoke?at?the?Air?Liquid?Interface.CORESTA?In?vitro?Toxicology?Task?Force.
[5]CORESTA?In?vitro?Toxicology?Task?Force，In?vitro?exposure?of?cells?to?smoke?at?the?air?liquid?interface.
http://www.coresta.org/Reports/IVT_TF_Report_Smoke_Air_Liquid_Interface.pdf.
[6]
http://www.vitrocell.com/.
[7]http://www.cultex.com/.
By literature search, do not see the open report identical with the present invention.
Summary of the invention:
The utility model purpose is to overcome the deficiency of traditional cigarette smoke capture method, and the full fume exposure culture apparatus of cigarette a kind of simple in structure, easy to operate is provided.
The full fume exposure culture apparatus of cigarette of the present utility model by the full fume exposure bottle of the cigarette which is provided with exhanst gas outlet (3) (6), and lower part place the flue gas of the full fume exposure bottle of cigarette (6) to import pipe close (7) two parts to form; Wherein: flue gas imports pipe close (7) and is made of smoke inlet (1), mixing air inlet (2), flue gas and air mixed chamber (4), dilution back flue gas carrying path (5).Device is formed with the Glass tubing blowing by experiment by traditional technology.
The utility model compared with prior art has simple in structurely, easy to operate, and the experiment difficulty reduces, and is with low cost, the convenient advantage that connects the orthoscopic smoking machine with the full fume exposure contamination of cigarette that obtains bacterium or cell.
Description of drawings:
Accompanying drawing is a structural representation of the present utility model.
Embodiment:
The full fume exposure culture apparatus of cigarette of the present utility model by the full fume exposure bottle 6 of the cigarette which is provided with exhanst gas outlet 3, and lower part place the flue gas of the full fume exposure bottle 6 of cigarette to import pipe close 7 two partss to form; Wherein: flue gas imports pipe close 7 and is made of smoke inlet 1, mixing air inlet 2, flue gas and air mixed chamber 4, dilution back flue gas carrying path 5.Device is formed with the Glass tubing blowing by experiment by traditional technology, and other parts that are attached thereto are that market is bought.
Full fume exposure device of cigarette of the present utility model and orthoscopic smoking machine be connected use, specific as follows:
Full fume exposure bottle 6 and flue gas importing pipe close 7 is standby in the high-pressure sterilizing pot sterilization 1.;
2. reacting cells or inoculum 10ml are placed full fume exposure bottle 6, insert flue gas and import pipe close 7;
3. straight line smoking machine flue gas incoming road sebific duct closely is connected on the smoke inlet 1;
4. filter one end closely is connected to exhanst gas outlet 3 places of full fume exposure bottle 6, again the filter the other end closely is connected with the smoking machine loop.
5. mixing air is connected to mixing air inlet 2 by sebific duct, flow velocity is by the minimum target flow velocity that transfers to.
6. set the automatic smoking machine program, the collection of beginning flue gas.
7. experiment finishes, and with the backward detaching device of erection sequence, deposits to carry out subsequent disposal reaction system is appropriate.
Practical application shows that really, this device reaches design requirements fully.
Claims (1)
1. full fume exposure culture apparatus of cigarette, it is characterized in that the full fume exposure culture apparatus of cigarette by the full fume exposure bottle of the cigarette which is provided with exhanst gas outlet (3) (6), and lower part place the flue gas of the full fume exposure bottle of cigarette (6) to import pipe close (7) two parts to form; Wherein: flue gas imports pipe close (7) and is made of smoke inlet (1), mixing air inlet (2), flue gas and air mixed chamber (4), dilution back flue gas carrying path (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200632516U CN202054824U (en) | 2011-03-11 | 2011-03-11 | Device for whole cigarette smoke exposure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200632516U CN202054824U (en) | 2011-03-11 | 2011-03-11 | Device for whole cigarette smoke exposure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202054824U true CN202054824U (en) | 2011-11-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011200632516U Expired - Lifetime CN202054824U (en) | 2011-03-11 | 2011-03-11 | Device for whole cigarette smoke exposure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202054824U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206572A (en) * | 2011-03-11 | 2011-10-05 | 云南烟草科学研究院 | Device for exposing cigarette mainstream smoke |
| CN102517204A (en) * | 2011-12-21 | 2012-06-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Cell gas poisoning apparatus |
| CN102586385A (en) * | 2012-01-20 | 2012-07-18 | 云南烟草科学研究院 | Gas-liquid contact type cigarette bacterial reverse mutation assay method under full smoke gas exposure |
| CN102676382A (en) * | 2012-05-29 | 2012-09-19 | 云南烟草科学研究院 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
| CN104713752A (en) * | 2015-03-31 | 2015-06-17 | 云南中烟工业有限责任公司 | Smoke collecting device and smoke collecting method for evaluating biological effects of electronic cigarette smoke |
-
2011
- 2011-03-11 CN CN2011200632516U patent/CN202054824U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206572A (en) * | 2011-03-11 | 2011-10-05 | 云南烟草科学研究院 | Device for exposing cigarette mainstream smoke |
| CN102517204A (en) * | 2011-12-21 | 2012-06-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Cell gas poisoning apparatus |
| CN102586385A (en) * | 2012-01-20 | 2012-07-18 | 云南烟草科学研究院 | Gas-liquid contact type cigarette bacterial reverse mutation assay method under full smoke gas exposure |
| CN102676382A (en) * | 2012-05-29 | 2012-09-19 | 云南烟草科学研究院 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
| CN104713752A (en) * | 2015-03-31 | 2015-06-17 | 云南中烟工业有限责任公司 | Smoke collecting device and smoke collecting method for evaluating biological effects of electronic cigarette smoke |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111130 |