CN102206572A - Device for exposing cigarette mainstream smoke - Google Patents
Device for exposing cigarette mainstream smoke Download PDFInfo
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- CN102206572A CN102206572A CN201110058671XA CN201110058671A CN102206572A CN 102206572 A CN102206572 A CN 102206572A CN 201110058671X A CN201110058671X A CN 201110058671XA CN 201110058671 A CN201110058671 A CN 201110058671A CN 102206572 A CN102206572 A CN 102206572A
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- flue gas
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- cigarette mainstream
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Abstract
The invention relates to a device for exposing cigarette mainstream smoke, and belongs to the technical field of gas exposure culture. The device comprises two parts of a cigarette mainstream smoke exposure bottle (6) provided with a smoke outlet (3), and a smoke guiding tube plug (7) of which the lower half is arranged in the cigarette mainstream smoke exposure bottle (6), wherein the smoke guiding tube plug (7) consists of a smoke inlet (1), a mixed air inlet (2), a smoke and air mixing chamber (4), and a diluted smoke conveying passage (5). Compared with the prior arts, the present device has the advantages of simple structure, convenient operation, reduction of experiment difficulty, low cost, and convenience of connection with a linear type smoking machine for realizing cigarette mainstream smoke exposure of bacteria or cells.
Description
Technical field:
The present invention relates to a kind of device that is used for the full fume exposure of cigarette, belong to gas exposure culture technique field.
Background technology:
The after chemical reaction in the very short time that flue gas produces of the aerosol that cigarette smoke is made up of several thousand kinds of chemical substances, these mixtures can produce countless qualitative changes and quantitative change [1], and wherein many components all have certain toxic action for cell or human body.External toxicologic study method is evaluated the potential impact [2] of experimental subjects to human body by measuring toxicant potential hazard or the damage of pair cell or DNA in a short time.Determination object comprises additive, tobacco extract, smoke condensate, flue gas gas phase thing and full flue gas.At present the external toxicologic study of cigarette smoke mainly concentrates on the genetoxic and the cytotoxicity testing research aspect of cigarette smoke, and these testing method are subjected to vast tobacco toxicology expert's consistent the approval, effectively the toxicity of evaluating cigarette product [3].
In the genetoxic and Cytotoxic test of the evaluating cigarette main flume of classics, the capture of flue gas sample mainly is on automatic smoking machine, capture the flue gas total particulate matter with cambridge filter, then the flue gas total particulate matter on the cambridge filter is eluted with DMSO, by cultivating, test the genetoxic and the cytotoxicity of flue gas with bacterium and cell.The advantage of this classical way is that the flue gas sample can prolonged preservation, shortcoming is the situation that the gained result can not represent authentic sample, because granule phase substance in test, the interaction of gas phase thing and volatile matter be not considered [4], after also having the long-time placement of flue gas, chemical ingredients can change.Therefore, just produced full fume exposure method.In full fume exposure method, the whole flue gases of cigarette directly are passed into the exposure container that cultivation has Salmonella typhimurium or test cell, its gas-liquid is dissolved each other or is allowed flue gas flow through the purpose that reaches full fume exposure with certain flow rate from media surface.This exposure chamber can in time reflect whole smoke components, and foreign tobacco company is developing full fume exposure device voluntarily, studies cytotoxicity and genetoxic [5] with evaluation of flue gas.Commercial Japanese Cultex[6 is arranged at present] and German Vitrocell[7] two brands, but complete assembly costs an arm and a leg, complicated operation.
The important research document of external full fume exposure device is as follows:
[1]Aufderheide，M.，An?efficient?approach?to?study?the?toxicological?effects?of?complex?mixtures.Experimental?and?Toxicologic?Pathology，2008.60(2008)：p.163-180.
[2]Aufderheide，M.and?H.Gressmann， A?modified?Ames?assay?reveals?the?mutagenicity?of?native?cigarette?mainstream?smoke?and?its?gas?vapo
[3]Aufderheide，M.，J.W.Knebel，and?D.Ritter，Novel?approaches?for?studying?pulmonary?toxicity?in?vitro.Toxicology?Letters，2003.140-141：p.205-211
[4]In?vitro?exposure?of?cells?to?Smoke?at?the?Air?Liquid?Interface.CORESTA?In?vitro?Toxicology?Task?Force.
[5]CORESTA?In?vitro?Toxicology?Task?Force，In?vitro?exposure?of?cells?to?smoke?at?the?air?liquid?interface.
http://www.coresta.org/Reports/IVT_TF_Report_Smoke_Air_Liquid_ Interface.pdf.
[6]
http://www.vitrocell.com/.
[7]http://www.cultex.com/.
By literature search, do not see the open report identical with the present invention.
Summary of the invention:
The object of the invention is to overcome the deficiency of traditional cigarette smoke capture method, and the full fume exposure culture apparatus of cigarette a kind of simple in structure, easy to operate is provided.
The full fume exposure culture apparatus of cigarette of the present invention by the full fume exposure bottle of the cigarette which is provided with exhanst gas outlet (3) (6), and lower part place the flue gas of the full fume exposure bottle of cigarette (6) to import pipe close (7) two parts to form; Wherein: flue gas imports pipe close (7) and is made of smoke inlet (1), mixing air inlet (2), flue gas and air mixed chamber (4), dilution back flue gas carrying path (5).Device is formed with the Glass tubing blowing by experiment by traditional technology.
The present invention compared with prior art has simple in structurely, easy to operate, and the experiment difficulty reduces, and is with low cost, the convenient advantage that connects the orthoscopic smoking machine with the full fume exposure contamination of cigarette that obtains bacterium or cell.
Description of drawings:
Accompanying drawing is a structural representation of the present invention.
Embodiment:
The full fume exposure culture apparatus of cigarette of the present invention by the full fume exposure bottle 6 of the cigarette which is provided with exhanst gas outlet 3, and lower part place the flue gas of the full fume exposure bottle 6 of cigarette to import pipe close 7 two partss to form; Wherein: flue gas imports pipe close 7 and is made of smoke inlet 1, mixing air inlet 2, flue gas and air mixed chamber 4, dilution back flue gas carrying path 5.Device is formed with the Glass tubing blowing by experiment by traditional technology, and other parts that are attached thereto are that market is bought.
Full fume exposure device of cigarette of the present invention and orthoscopic smoking machine be connected use, specific as follows:
Full fume exposure bottle 6 and flue gas importing pipe close 7 is standby in the high-pressure sterilizing pot sterilization 1.;
2. reacting cells or inoculum 10ml are placed full fume exposure bottle 6, insert flue gas and import pipe close 7;
3. straight line smoking machine flue gas incoming road sebific duct closely is connected on the smoke inlet 1;
4. filter one end closely is connected to exhanst gas outlet 3 places of full fume exposure bottle 6, again the filter the other end closely is connected with the smoking machine loop.
5. mixing air is connected to mixing air inlet 2 by sebific duct, flow velocity is by the minimum target flow velocity that transfers to.
6. set the automatic smoking machine program, the collection of beginning flue gas.
7. experiment finishes, and with the backward detaching device of erection sequence, deposits to carry out subsequent disposal reaction system is appropriate.
Practical application shows that really, this device reaches design requirements fully.
Claims (1)
1. full fume exposure culture apparatus of cigarette, it is characterized in that the full fume exposure culture apparatus of cigarette by the full fume exposure bottle of the cigarette which is provided with exhanst gas outlet (3) (6), and lower part place the flue gas of the full fume exposure bottle of cigarette (6) to import pipe close (7) two parts to form; Wherein: flue gas imports pipe close (7) and is made of smoke inlet (1), mixing air inlet (2), flue gas and air mixed chamber (4), dilution back flue gas carrying path (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110058671XA CN102206572A (en) | 2011-03-11 | 2011-03-11 | Device for exposing cigarette mainstream smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110058671XA CN102206572A (en) | 2011-03-11 | 2011-03-11 | Device for exposing cigarette mainstream smoke |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102206572A true CN102206572A (en) | 2011-10-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110058671XA Pending CN102206572A (en) | 2011-03-11 | 2011-03-11 | Device for exposing cigarette mainstream smoke |
Country Status (1)
| Country | Link |
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| CN (1) | CN102206572A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495183A (en) * | 2011-12-16 | 2012-06-13 | 云南烟草科学研究院 | Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure |
| CN107805611A (en) * | 2017-11-14 | 2018-03-16 | 郑州轻工业学院 | A kind of full fume exposure device and method of cell |
| CN109557254A (en) * | 2018-11-14 | 2019-04-02 | 云南中烟工业有限责任公司 | A method of establishing standardization cigarette smoke mouse mouth and nose process for exposing |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999036505A1 (en) * | 1998-01-19 | 1999-07-22 | Ulrich Mohr | Culture device and method for cultivating cells or tissue components |
| CN201314878Y (en) * | 2008-11-05 | 2009-09-23 | 湖北中烟工业有限责任公司 | Device for catching gas-phase free radical in cigarette mainstream smoke |
| CN101580869A (en) * | 2009-07-01 | 2009-11-18 | 中国烟草总公司郑州烟草研究院 | Cigarette mainstream smoke genotoxicity measurement method |
| CN201560188U (en) * | 2009-09-17 | 2010-08-25 | 中国烟草总公司郑州烟草研究院 | Full smoke exposure device of cigarette |
| CN202054824U (en) * | 2011-03-11 | 2011-11-30 | 云南烟草科学研究院 | Device for whole cigarette smoke exposure |
-
2011
- 2011-03-11 CN CN201110058671XA patent/CN102206572A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999036505A1 (en) * | 1998-01-19 | 1999-07-22 | Ulrich Mohr | Culture device and method for cultivating cells or tissue components |
| CN201314878Y (en) * | 2008-11-05 | 2009-09-23 | 湖北中烟工业有限责任公司 | Device for catching gas-phase free radical in cigarette mainstream smoke |
| CN101580869A (en) * | 2009-07-01 | 2009-11-18 | 中国烟草总公司郑州烟草研究院 | Cigarette mainstream smoke genotoxicity measurement method |
| CN201560188U (en) * | 2009-09-17 | 2010-08-25 | 中国烟草总公司郑州烟草研究院 | Full smoke exposure device of cigarette |
| CN202054824U (en) * | 2011-03-11 | 2011-11-30 | 云南烟草科学研究院 | Device for whole cigarette smoke exposure |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495183A (en) * | 2011-12-16 | 2012-06-13 | 云南烟草科学研究院 | Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure |
| CN102495183B (en) * | 2011-12-16 | 2014-08-13 | 云南烟草科学研究院 | Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure |
| CN107805611A (en) * | 2017-11-14 | 2018-03-16 | 郑州轻工业学院 | A kind of full fume exposure device and method of cell |
| CN107805611B (en) * | 2017-11-14 | 2021-01-08 | 郑州轻工业学院 | Cell whole smoke exposure device and method |
| CN109557254A (en) * | 2018-11-14 | 2019-04-02 | 云南中烟工业有限责任公司 | A method of establishing standardization cigarette smoke mouse mouth and nose process for exposing |
| CN109557254B (en) * | 2018-11-14 | 2021-08-03 | 云南中烟工业有限责任公司 | Method for establishing standardized cigarette smoke mouse mouth and nose exposure method |
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Application publication date: 20111005 |